SUMMARISING RUN PARAMETERS ========================== Input filename: L77-Saccharomyces_paradoxus-GC_only.end1.36mers.fastq.gz Trimming mode: paired-end Trim Galore version: 0.4.4 Cutadapt version: 1.15 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp All sequences will be trimmed by 1 bp on their 3' end to avoid problems with invalid paired-end alignments with Bowtie 1 Output file will be GZIP compressed This is cutadapt 1.15 with Python 2.7.14+ Command line parameters: -f fastq -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC L77-Saccharomyces_paradoxus-GC_only.end1.36mers.fastq.gz Running on 1 core Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 142.67 s (22 us/read; 2.75 M reads/minute). === Summary === Total reads processed: 6,527,676 Reads with adapters: 3,599,213 (55.1%) Reads written (passing filters): 6,527,676 (100.0%) Total basepairs processed: 234,996,336 bp Quality-trimmed: 2,362,331 bp (1.0%) Total written (filtered): 216,682,713 bp (92.2%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 3599213 times. No. of allowed errors: 0-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 57.5% C: 14.2% G: 1.3% T: 27.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 1 2629146 1631919.0 0 2629146 2 92169 407979.8 0 92169 3 51685 101994.9 0 51685 4 41492 25498.7 0 41492 5 33342 6374.7 0 33342 6 31815 1593.7 0 31815 7 30273 398.4 0 30273 8 29953 99.6 0 29953 9 31057 24.9 0 30834 223 10 40036 6.2 1 34419 5617 11 28981 1.6 1 23119 5862 12 35378 0.4 1 29485 5893 13 39873 0.1 1 32999 6874 14 30340 0.1 1 24202 6138 15 40466 0.1 1 33293 7173 16 27115 0.1 1 21609 5506 17 37011 0.1 1 30221 6790 18 36365 0.1 1 30104 6261 19 35697 0.1 1 29706 5991 20 36179 0.1 1 30080 6099 21 36979 0.1 1 30782 6197 22 43582 0.1 1 37357 6225 23 36115 0.1 1 30499 5616 24 31752 0.1 1 27111 4641 25 20317 0.1 1 17332 2985 26 11775 0.1 1 9865 1910 27 9003 0.1 1 7323 1680 28 10555 0.1 1 7970 2585 29 12755 0.1 1 8593 4162 30 12674 0.1 1 7129 5545 31 8156 0.1 1 3250 4906 32 5673 0.1 1 1292 4381 33 1453 0.1 1 64 1389 34 44 0.1 1 3 41 35 5 0.1 1 0 5 36 2 0.1 1 1 1 RUN STATISTICS FOR INPUT FILE: L77-Saccharomyces_paradoxus-GC_only.end1.36mers.fastq.gz ============================================= 6527676 sequences processed in total