SUMMARISING RUN PARAMETERS
==========================
Input filename: KO-13006-2.end1.fastq.gz
Trimming mode: paired-end
Trim Galore version: 0.4.4
Cutadapt version: 1.16
Quality Phred score cutoff: 20
Quality encoding type selected: ASCII+33
Adapter sequence: 'CTGTCTCTTATA' (Nextera Transposase sequence; auto-detected)
Maximum trimming error rate: 0.1 (default)
Minimum required adapter overlap (stringency): 1 bp
Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp
All sequences will be trimmed by 1 bp on their 3' end to avoid problems with invalid paired-end alignments with Bowtie 1
Output file will be GZIP compressed


This is cutadapt 1.16 with Python 2.7.13
Command line parameters: -f fastq -e 0.1 -q 20 -O 1 -a CTGTCTCTTATA KO-13006-2.end1.fastq.gz
Running on 1 core
Trimming 1 adapter with at most 10.0% errors in single-end mode ...
Finished in 764.77 s (35 us/read; 1.72 M reads/minute).

=== Summary ===

Total reads processed:              21,881,230
Reads with adapters:                 8,634,420 (39.5%)
Reads written (passing filters):    21,881,230 (100.0%)

Total basepairs processed: 1,405,499,277 bp
Quality-trimmed:                  12,671 bp (0.0%)
Total written (filtered):  1,390,359,567 bp (98.9%)

=== Adapter 1 ===

Sequence: CTGTCTCTTATA; Type: regular 3'; Length: 12; Trimmed: 8634420 times.

No. of allowed errors:
0-9 bp: 0; 10-12 bp: 1

Bases preceding removed adapters:
  A: 26.9%
  C: 27.8%
  G: 23.3%
  T: 22.0%
  none/other: 0.0%

Overview of removed sequences
length	count	expect	max.err	error counts
1	6745810	5470307.5	0	6745810
2	1280618	1367576.9	0	1280618
3	348845	341894.2	0	348845
4	76620	85473.6	0	76620
5	20033	21368.4	0	20033
6	11560	5342.1	0	11560
7	4195	1335.5	0	4195
8	3285	333.9	0	3285
9	3243	83.5	0	2546 697
10	3093	20.9	1	2240 853
11	3723	5.2	1	2468 1255
12	3198	1.3	1	2531 667
13	3139	1.3	1	2590 549
14	3496	1.3	1	2383 1113
15	3105	1.3	1	2145 960
16	2599	1.3	1	2068 531
17	2527	1.3	1	2090 437
18	2641	1.3	1	2447 194
19	2938	1.3	1	2619 319
20	3400	1.3	1	3002 398
21	3582	1.3	1	3382 200
22	4634	1.3	1	3809 825
23	4889	1.3	1	4631 258
24	4658	1.3	1	4412 246
25	3757	1.3	1	3597 160
26	3238	1.3	1	3009 229
27	3111	1.3	1	2803 308
28	3286	1.3	1	3058 228
29	4008	1.3	1	3774 234
30	4507	1.3	1	4327 180
31	4407	1.3	1	4225 182
32	4765	1.3	1	4575 190
33	6270	1.3	1	5957 313
34	6972	1.3	1	6783 189
35	5962	1.3	1	5775 187
36	4642	1.3	1	4429 213
37	4215	1.3	1	4044 171
38	4331	1.3	1	4064 267
39	4013	1.3	1	3840 173
40	2757	1.3	1	2589 168
41	1655	1.3	1	1497 158
42	959	1.3	1	819 140
43	1142	1.3	1	693 449
44	443	1.3	1	258 185
45	654	1.3	1	330 324
46	538	1.3	1	341 197
47	473	1.3	1	317 156
48	852	1.3	1	525 327
49	1140	1.3	1	965 175
50	1659	1.3	1	89 1570
51	333	1.3	1	19 314
52	224	1.3	1	11 213
53	461	1.3	1	4 457
54	116	1.3	1	10 106
55	432	1.3	1	3 429
56	235	1.3	1	7 228
57	127	1.3	1	3 124
58	371	1.3	1	1 370
59	231	1.3	1	0 231
60	1117	1.3	1	4 1113
61	143	1.3	1	3 140
62	242	1.3	1	43 199
63	423	1.3	1	2 421
64	99	1.3	1	18 81
65	123	1.3	1	6 117
66	175	1.3	1	4 171
67	199	1.3	1	6 193
68	383	1.3	1	8 375
69	60	1.3	1	7 53
70	71	1.3	1	1 70
71	47	1.3	1	2 45
72	87	1.3	1	7 80
73	409	1.3	1	22 387
74	147	1.3	1	58 89
75	2501	1.3	1	2297 204
76	77	1.3	1	2 75


RUN STATISTICS FOR INPUT FILE: KO-13006-2.end1.fastq.gz
=============================================
21881230 sequences processed in total