SUMMARISING RUN PARAMETERS ========================== Input filename: A-15173-2.end1.fastq.gz Trimming mode: paired-end Trim Galore version: 0.4.4 Cutadapt version: 1.16 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Adapter sequence: 'CTGTCTCTTATA' (Nextera Transposase sequence; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp All sequences will be trimmed by 1 bp on their 3' end to avoid problems with invalid paired-end alignments with Bowtie 1 Output file will be GZIP compressed This is cutadapt 1.16 with Python 2.7.13 Command line parameters: -f fastq -e 0.1 -q 20 -O 1 -a CTGTCTCTTATA A-15173-2.end1.fastq.gz Running on 1 core Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 894.47 s (37 us/read; 1.64 M reads/minute). === Summary === Total reads processed: 24,422,282 Reads with adapters: 9,569,245 (39.2%) Reads written (passing filters): 24,422,282 (100.0%) Total basepairs processed: 1,567,897,430 bp Quality-trimmed: 11,217 bp (0.0%) Total written (filtered): 1,551,355,876 bp (98.9%) === Adapter 1 === Sequence: CTGTCTCTTATA; Type: regular 3'; Length: 12; Trimmed: 9569245 times. No. of allowed errors: 0-9 bp: 0; 10-12 bp: 1 Bases preceding removed adapters: A: 27.6% C: 27.5% G: 22.9% T: 22.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 1 7483108 6105570.5 0 7483108 2 1426349 1526392.6 0 1426349 3 379380 381598.2 0 379380 4 83713 95399.5 0 83713 5 21256 23849.9 0 21256 6 11564 5962.5 0 11564 7 4498 1490.6 0 4498 8 3540 372.7 0 3540 9 3597 93.2 0 2814 783 10 3673 23.3 1 2601 1072 11 4163 5.8 1 2670 1493 12 3540 1.5 1 2792 748 13 3471 1.5 1 2924 547 14 4157 1.5 1 2640 1517 15 3740 1.5 1 2426 1314 16 2937 1.5 1 2303 634 17 2869 1.5 1 2278 591 18 2832 1.5 1 2636 196 19 3314 1.5 1 2911 403 20 3722 1.5 1 3289 433 21 4053 1.5 1 3798 255 22 5425 1.5 1 4243 1182 23 5633 1.5 1 5331 302 24 5589 1.5 1 5246 343 25 4608 1.5 1 4411 197 26 3537 1.5 1 3294 243 27 3486 1.5 1 3161 325 28 3799 1.5 1 3455 344 29 4464 1.5 1 4147 317 30 4950 1.5 1 4746 204 31 4814 1.5 1 4588 226 32 5083 1.5 1 4848 235 33 7156 1.5 1 6816 340 34 7648 1.5 1 7409 239 35 6749 1.5 1 6536 213 36 4932 1.5 1 4699 233 37 4023 1.5 1 3846 177 38 3870 1.5 1 3594 276 39 3701 1.5 1 3509 192 40 2360 1.5 1 2157 203 41 1304 1.5 1 1136 168 42 783 1.5 1 660 123 43 990 1.5 1 480 510 44 451 1.5 1 279 172 45 739 1.5 1 331 408 46 564 1.5 1 297 267 47 506 1.5 1 315 191 48 859 1.5 1 489 370 49 1292 1.5 1 1045 247 50 1907 1.5 1 116 1791 51 362 1.5 1 34 328 52 276 1.5 1 8 268 53 482 1.5 1 6 476 54 148 1.5 1 9 139 55 488 1.5 1 3 485 56 244 1.5 1 4 240 57 196 1.5 1 0 196 58 437 1.5 1 3 434 59 296 1.5 1 0 296 60 1164 1.5 1 0 1164 61 169 1.5 1 1 168 62 220 1.5 1 16 204 63 408 1.5 1 3 405 64 118 1.5 1 5 113 65 147 1.5 1 4 143 66 157 1.5 1 1 156 67 268 1.5 1 2 266 68 405 1.5 1 4 401 69 65 1.5 1 3 62 70 91 1.5 1 3 88 71 50 1.5 1 3 47 72 84 1.5 1 9 75 73 420 1.5 1 11 409 74 128 1.5 1 40 88 75 1672 1.5 1 1501 171 76 52 1.5 1 1 51 RUN STATISTICS FOR INPUT FILE: A-15173-2.end1.fastq.gz ============================================= 24422282 sequences processed in total