SUMMARISING RUN PARAMETERS ========================== Input filename: A-15173-1.end1.fastq.gz Trimming mode: paired-end Trim Galore version: 0.4.4 Cutadapt version: 1.16 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Adapter sequence: 'CTGTCTCTTATA' (Nextera Transposase sequence; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp All sequences will be trimmed by 1 bp on their 3' end to avoid problems with invalid paired-end alignments with Bowtie 1 Output file will be GZIP compressed This is cutadapt 1.16 with Python 2.7.13 Command line parameters: -f fastq -e 0.1 -q 20 -O 1 -a CTGTCTCTTATA A-15173-1.end1.fastq.gz Running on 1 core Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1117.08 s (36 us/read; 1.65 M reads/minute). === Summary === Total reads processed: 30,709,434 Reads with adapters: 11,974,401 (39.0%) Reads written (passing filters): 30,709,434 (100.0%) Total basepairs processed: 1,983,389,762 bp Quality-trimmed: 10,880 bp (0.0%) Total written (filtered): 1,964,211,786 bp (99.0%) === Adapter 1 === Sequence: CTGTCTCTTATA; Type: regular 3'; Length: 12; Trimmed: 11974401 times. No. of allowed errors: 0-9 bp: 0; 10-12 bp: 1 Bases preceding removed adapters: A: 27.5% C: 27.4% G: 23.0% T: 22.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 1 9386372 7677358.5 0 9386372 2 1812681 1919339.6 0 1812681 3 482391 479834.9 0 482391 4 105921 119958.7 0 105921 5 26354 29989.7 0 26354 6 14541 7497.4 0 14541 7 4766 1874.4 0 4766 8 3670 468.6 0 3670 9 3629 117.1 0 2687 942 10 3479 29.3 1 2238 1241 11 4103 7.3 1 2311 1792 12 3466 1.8 1 2541 925 13 3276 1.8 1 2509 767 14 4005 1.8 1 2253 1752 15 3396 1.8 1 1965 1431 16 2620 1.8 1 1908 712 17 2658 1.8 1 1956 702 18 2380 1.8 1 2136 244 19 2939 1.8 1 2406 533 20 3329 1.8 1 2762 567 21 3510 1.8 1 3215 295 22 5036 1.8 1 3698 1338 23 4980 1.8 1 4650 330 24 4981 1.8 1 4590 391 25 3776 1.8 1 3573 203 26 2891 1.8 1 2599 292 27 2979 1.8 1 2495 484 28 3069 1.8 1 2703 366 29 3555 1.8 1 3197 358 30 4014 1.8 1 3754 260 31 3945 1.8 1 3732 213 32 4149 1.8 1 3921 228 33 5900 1.8 1 5486 414 34 6415 1.8 1 6156 259 35 5384 1.8 1 5163 221 36 3767 1.8 1 3510 257 37 3104 1.8 1 2896 208 38 3223 1.8 1 2896 327 39 2625 1.8 1 2414 211 40 1892 1.8 1 1642 250 41 1061 1.8 1 840 221 42 687 1.8 1 505 182 43 1069 1.8 1 436 633 44 420 1.8 1 204 216 45 707 1.8 1 204 503 46 519 1.8 1 216 303 47 427 1.8 1 189 238 48 767 1.8 1 312 455 49 829 1.8 1 591 238 50 2310 1.8 1 58 2252 51 485 1.8 1 18 467 52 343 1.8 1 6 337 53 657 1.8 1 1 656 54 157 1.8 1 1 156 55 666 1.8 1 3 663 56 273 1.8 1 3 270 57 207 1.8 1 2 205 58 559 1.8 1 0 559 59 382 1.8 1 2 380 60 1506 1.8 1 3 1503 61 253 1.8 1 1 252 62 301 1.8 1 19 282 63 623 1.8 1 3 620 64 145 1.8 1 7 138 65 156 1.8 1 2 154 66 205 1.8 1 4 201 67 289 1.8 1 2 287 68 563 1.8 1 10 553 69 113 1.8 1 4 109 70 86 1.8 1 2 84 71 66 1.8 1 5 61 72 113 1.8 1 9 104 73 529 1.8 1 12 517 74 161 1.8 1 30 131 75 1519 1.8 1 1261 258 76 77 1.8 1 5 72 RUN STATISTICS FOR INPUT FILE: A-15173-1.end1.fastq.gz ============================================= 30709434 sequences processed in total