SUMMARISING RUN PARAMETERS ========================== Input filename: A-15171-2.end1.fastq.gz Trimming mode: paired-end Trim Galore version: 0.4.4 Cutadapt version: 1.16 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Adapter sequence: 'CTGTCTCTTATA' (Nextera Transposase sequence; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp All sequences will be trimmed by 1 bp on their 3' end to avoid problems with invalid paired-end alignments with Bowtie 1 Output file will be GZIP compressed This is cutadapt 1.16 with Python 2.7.13 Command line parameters: -f fastq -e 0.1 -q 20 -O 1 -a CTGTCTCTTATA A-15171-2.end1.fastq.gz Running on 1 core Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 917.36 s (36 us/read; 1.66 M reads/minute). === Summary === Total reads processed: 25,447,185 Reads with adapters: 10,052,010 (39.5%) Reads written (passing filters): 25,447,185 (100.0%) Total basepairs processed: 1,626,786,722 bp Quality-trimmed: 13,054 bp (0.0%) Total written (filtered): 1,609,015,363 bp (98.9%) === Adapter 1 === Sequence: CTGTCTCTTATA; Type: regular 3'; Length: 12; Trimmed: 10052010 times. No. of allowed errors: 0-9 bp: 0; 10-12 bp: 1 Bases preceding removed adapters: A: 27.6% C: 27.7% G: 22.9% T: 21.8% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 1 7881781 6361796.2 0 7881781 2 1476220 1590449.1 0 1476220 3 385108 397612.3 0 385108 4 85123 99403.1 0 85123 5 21558 24850.8 0 21558 6 11952 6212.7 0 11952 7 4882 1553.2 0 4882 8 4281 388.3 0 4281 9 4144 97.1 0 3410 734 10 4069 24.3 1 3074 995 11 4786 6.1 1 3224 1562 12 4254 1.5 1 3441 813 13 4231 1.5 1 3602 629 14 4700 1.5 1 3216 1484 15 4295 1.5 1 2882 1413 16 3522 1.5 1 2781 741 17 3499 1.5 1 2855 644 18 3296 1.5 1 3101 195 19 3967 1.5 1 3506 461 20 4334 1.5 1 3836 498 21 4816 1.5 1 4559 257 22 6189 1.5 1 4981 1208 23 6388 1.5 1 6038 350 24 6229 1.5 1 5849 380 25 5340 1.5 1 5112 228 26 4187 1.5 1 3965 222 27 4293 1.5 1 3884 409 28 4478 1.5 1 4117 361 29 5416 1.5 1 5084 332 30 5849 1.5 1 5599 250 31 5690 1.5 1 5484 206 32 5980 1.5 1 5751 229 33 8201 1.5 1 7880 321 34 8599 1.5 1 8354 245 35 7836 1.5 1 7613 223 36 5916 1.5 1 5666 250 37 4861 1.5 1 4689 172 38 4823 1.5 1 4522 301 39 4599 1.5 1 4395 204 40 2901 1.5 1 2689 212 41 1568 1.5 1 1408 160 42 916 1.5 1 775 141 43 1124 1.5 1 629 495 44 456 1.5 1 303 153 45 810 1.5 1 366 444 46 626 1.5 1 357 269 47 516 1.5 1 320 196 48 949 1.5 1 534 415 49 1256 1.5 1 1009 247 50 2164 1.5 1 120 2044 51 423 1.5 1 24 399 52 270 1.5 1 10 260 53 559 1.5 1 6 553 54 123 1.5 1 4 119 55 481 1.5 1 5 476 56 235 1.5 1 1 234 57 210 1.5 1 6 204 58 411 1.5 1 0 411 59 323 1.5 1 1 322 60 1332 1.5 1 2 1330 61 184 1.5 1 2 182 62 239 1.5 1 18 221 63 441 1.5 1 1 440 64 128 1.5 1 10 118 65 146 1.5 1 6 140 66 135 1.5 1 4 131 67 247 1.5 1 4 243 68 417 1.5 1 3 414 69 87 1.5 1 3 84 70 88 1.5 1 6 82 71 45 1.5 1 5 40 72 86 1.5 1 11 75 73 439 1.5 1 10 429 74 143 1.5 1 46 97 75 1775 1.5 1 1573 202 76 65 1.5 1 1 64 RUN STATISTICS FOR INPUT FILE: A-15171-2.end1.fastq.gz ============================================= 25447185 sequences processed in total