SUMMARISING RUN PARAMETERS ========================== Input filename: A-13189-2.end1.fastq.gz Trimming mode: paired-end Trim Galore version: 0.4.4 Cutadapt version: 1.16 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Adapter sequence: 'CTGTCTCTTATA' (Nextera Transposase sequence; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp All sequences will be trimmed by 1 bp on their 3' end to avoid problems with invalid paired-end alignments with Bowtie 1 Output file will be GZIP compressed This is cutadapt 1.16 with Python 2.7.13 Command line parameters: -f fastq -e 0.1 -q 20 -O 1 -a CTGTCTCTTATA A-13189-2.end1.fastq.gz Running on 1 core Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 904.74 s (36 us/read; 1.68 M reads/minute). === Summary === Total reads processed: 25,336,192 Reads with adapters: 9,921,202 (39.2%) Reads written (passing filters): 25,336,192 (100.0%) Total basepairs processed: 1,637,479,649 bp Quality-trimmed: 12,294 bp (0.0%) Total written (filtered): 1,620,622,234 bp (99.0%) === Adapter 1 === Sequence: CTGTCTCTTATA; Type: regular 3'; Length: 12; Trimmed: 9921202 times. No. of allowed errors: 0-9 bp: 0; 10-12 bp: 1 Bases preceding removed adapters: A: 27.2% C: 27.9% G: 23.0% T: 21.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 1 7760883 6334048.0 0 7760883 2 1490069 1583512.0 0 1490069 3 390659 395878.0 0 390659 4 87317 98969.5 0 87317 5 22071 24742.4 0 22071 6 12207 6185.6 0 12207 7 4585 1546.4 0 4585 8 3709 386.6 0 3709 9 3612 96.6 0 2883 729 10 3548 24.2 1 2532 1016 11 4202 6.0 1 2699 1503 12 3589 1.5 1 2785 804 13 3535 1.5 1 2891 644 14 3953 1.5 1 2615 1338 15 3556 1.5 1 2386 1170 16 2732 1.5 1 2183 549 17 2749 1.5 1 2165 584 18 2679 1.5 1 2484 195 19 3221 1.5 1 2819 402 20 3409 1.5 1 2977 432 21 3728 1.5 1 3472 256 22 5305 1.5 1 4178 1127 23 5187 1.5 1 4897 290 24 5229 1.5 1 4913 316 25 4196 1.5 1 3988 208 26 3362 1.5 1 3093 269 27 3374 1.5 1 2984 390 28 3497 1.5 1 3185 312 29 4116 1.5 1 3811 305 30 4410 1.5 1 4207 203 31 4459 1.5 1 4247 212 32 4587 1.5 1 4356 231 33 6702 1.5 1 6342 360 34 6985 1.5 1 6748 237 35 6255 1.5 1 6071 184 36 4769 1.5 1 4502 267 37 3564 1.5 1 3401 163 38 3951 1.5 1 3679 272 39 3691 1.5 1 3501 190 40 2711 1.5 1 2495 216 41 1439 1.5 1 1253 186 42 1002 1.5 1 845 157 43 1326 1.5 1 724 602 44 507 1.5 1 306 201 45 676 1.5 1 268 408 46 555 1.5 1 297 258 47 439 1.5 1 259 180 48 822 1.5 1 421 401 49 1074 1.5 1 853 221 50 2001 1.5 1 86 1915 51 395 1.5 1 26 369 52 270 1.5 1 12 258 53 582 1.5 1 7 575 54 108 1.5 1 8 100 55 498 1.5 1 3 495 56 231 1.5 1 6 225 57 151 1.5 1 4 147 58 549 1.5 1 0 549 59 295 1.5 1 0 295 60 1443 1.5 1 2 1441 61 194 1.5 1 5 189 62 251 1.5 1 34 217 63 573 1.5 1 6 567 64 108 1.5 1 9 99 65 145 1.5 1 6 139 66 164 1.5 1 5 159 67 260 1.5 1 5 255 68 437 1.5 1 5 432 69 71 1.5 1 2 69 70 77 1.5 1 2 75 71 41 1.5 1 1 40 72 110 1.5 1 14 96 73 433 1.5 1 9 424 74 159 1.5 1 47 112 75 1390 1.5 1 1181 209 76 63 1.5 1 4 59 RUN STATISTICS FOR INPUT FILE: A-13189-2.end1.fastq.gz ============================================= 25336192 sequences processed in total