SUMMARISING RUN PARAMETERS ========================== Input filename: Undetermined_L001_R1_001.fastq.gz Trimming mode: paired-end Trim Galore version: 0.4.4 Cutadapt version: 1.15 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Adapter sequence: 'CTGTCTCTTATA' (Nextera Transposase sequence; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Output file will be GZIP compressed This is cutadapt 1.15 with Python 2.7.14+ Command line parameters: -f fastq -e 0.1 -q 20 -O 1 -a CTGTCTCTTATA Undetermined_L001_R1_001.fastq.gz Running on 1 core Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 262.40 s (23 us/read; 2.58 M reads/minute). === Summary === Total reads processed: 11,279,426 Reads with adapters: 1,493,643 (13.2%) Reads written (passing filters): 11,279,426 (100.0%) Total basepairs processed: 857,236,376 bp Quality-trimmed: 90,225,611 bp (10.5%) Total written (filtered): 761,994,530 bp (88.9%) === Adapter 1 === Sequence: CTGTCTCTTATA; Type: regular 3'; Length: 12; Trimmed: 1493643 times. No. of allowed errors: 0-9 bp: 0; 10-12 bp: 1 Bases preceding removed adapters: A: 28.3% C: 35.3% G: 15.3% T: 20.7% none/other: 0.3% Overview of removed sequences length count expect max.err error counts 1 1167328 2819856.5 0 1167328 2 169430 704964.1 0 169430 3 56785 176241.0 0 56785 4 12491 44060.3 0 12491 5 6964 11015.1 0 6964 6 1356 2753.8 0 1356 7 1996 688.4 0 1996 8 1908 172.1 0 1908 9 1273 43.0 0 837 436 10 3865 10.8 1 2698 1167 11 1913 2.7 1 210 1703 12 3383 0.7 1 2323 1060 13 2171 0.7 1 1400 771 14 3473 0.7 1 2509 964 15 1607 0.7 1 998 609 16 3639 0.7 1 2776 863 17 823 0.7 1 602 221 18 1205 0.7 1 1002 203 19 253 0.7 1 201 52 20 791 0.7 1 583 208 21 1275 0.7 1 958 317 22 323 0.7 1 252 71 23 1101 0.7 1 912 189 24 212 0.7 1 163 49 25 359 0.7 1 296 63 26 406 0.7 1 331 75 27 351 0.7 1 293 58 28 658 0.7 1 562 96 29 362 0.7 1 310 52 30 95 0.7 1 75 20 31 652 0.7 1 590 62 32 68 0.7 1 40 28 33 575 0.7 1 514 61 34 265 0.7 1 248 17 35 324 0.7 1 305 19 36 439 0.7 1 408 31 37 409 0.7 1 394 15 38 466 0.7 1 443 23 39 376 0.7 1 366 10 40 512 0.7 1 505 7 41 453 0.7 1 445 8 42 488 0.7 1 465 23 43 1476 0.7 1 1454 22 44 193 0.7 1 180 13 45 463 0.7 1 444 19 46 328 0.7 1 311 17 47 564 0.7 1 540 24 48 505 0.7 1 481 24 49 387 0.7 1 375 12 50 1044 0.7 1 1016 28 51 671 0.7 1 659 12 52 855 0.7 1 808 47 53 648 0.7 1 627 21 54 789 0.7 1 775 14 55 1434 0.7 1 1425 9 56 331 0.7 1 322 9 57 1539 0.7 1 1527 12 58 1087 0.7 1 1075 12 59 672 0.7 1 665 7 60 1957 0.7 1 1945 12 61 638 0.7 1 621 17 62 1386 0.7 1 1373 13 63 1961 0.7 1 1941 20 64 1126 0.7 1 1120 6 65 668 0.7 1 662 6 66 1153 0.7 1 1147 6 67 4301 0.7 1 4275 26 68 1183 0.7 1 1174 9 69 2181 0.7 1 2161 20 70 1119 0.7 1 1110 9 71 1981 0.7 1 1956 25 72 1813 0.7 1 1788 25 73 275 0.7 1 242 33 74 363 0.7 1 327 36 75 936 0.7 1 822 114 76 4793 0.7 1 535 4258 RUN STATISTICS FOR INPUT FILE: Undetermined_L001_R1_001.fastq.gz ============================================= 11279426 sequences processed in total