SUMMARISING RUN PARAMETERS ========================== Input filename: N1-H-iG-IgG_S10_L002_R2_001.fastq.gz Trimming mode: single-end Trim Galore version: 0.4.4 Cutadapt version: 1.18 Quality Phred score cutoff: 0 Quality encoding type selected: ASCII+33 Adapter sequence: 'CTGTCTCTTATA' (Nextera Transposase sequence; user defined) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length before a sequence gets removed: 20 bp Output file will be GZIP compressed This is cutadapt 1.18 with Python 2.7.13 Command line parameters: -f fastq -e 0.1 -q 0 -O 1 -a CTGTCTCTTATA N1-H-iG-IgG_S10_L002_R2_001.fastq.gz Processing reads on 1 core in single-end mode ... Finished in 214.22 s (37 us/read; 1.60 M reads/minute). === Summary === Total reads processed: 5,717,719 Reads with adapters: 3,353,792 (58.7%) Reads written (passing filters): 5,717,719 (100.0%) Total basepairs processed: 863,375,569 bp Quality-trimmed: 0 bp (0.0%) Total written (filtered): 725,730,469 bp (84.1%) === Adapter 1 === Sequence: CTGTCTCTTATA; Type: regular 3'; Length: 12; Trimmed: 3353792 times. No. of allowed errors: 0-9 bp: 0; 10-12 bp: 1 Bases preceding removed adapters: A: 11.9% C: 44.8% G: 24.5% T: 18.8% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 1 891116 1429429.8 0 891116 2 257427 357357.4 0 257427 3 89655 89339.4 0 89655 4 34765 22334.8 0 34765 5 16591 5583.7 0 16591 6 13633 1395.9 0 13633 7 12337 349.0 0 12337 8 10752 87.2 0 10752 9 9840 21.8 0 9714 126 10 13091 5.5 1 11821 1270 11 14638 1.4 1 13663 975 12 12886 0.3 1 12323 563 13 15274 0.3 1 14652 622 14 16772 0.3 1 16186 586 15 14076 0.3 1 13561 515 16 13259 0.3 1 12765 494 17 11678 0.3 1 11250 428 18 10610 0.3 1 10214 396 19 8908 0.3 1 8614 294 20 10361 0.3 1 10059 302 21 12785 0.3 1 12412 373 22 13047 0.3 1 12538 509 23 14341 0.3 1 13940 401 24 14927 0.3 1 14468 459 25 16010 0.3 1 15541 469 26 15445 0.3 1 15021 424 27 13770 0.3 1 13380 390 28 10956 0.3 1 10553 403 29 10898 0.3 1 10662 236 30 10340 0.3 1 9964 376 31 11158 0.3 1 10889 269 32 14053 0.3 1 13554 499 33 17020 0.3 1 16479 541 34 19312 0.3 1 18697 615 35 20461 0.3 1 19758 703 36 16213 0.3 1 15858 355 37 15534 0.3 1 15075 459 38 12771 0.3 1 12492 279 39 10278 0.3 1 10082 196 40 11103 0.3 1 10780 323 41 11373 0.3 1 11114 259 42 13113 0.3 1 12707 406 43 17183 0.3 1 16755 428 44 19888 0.3 1 19432 456 45 22461 0.3 1 21882 579 46 23032 0.3 1 22557 475 47 18558 0.3 1 18183 375 48 14535 0.3 1 13958 577 49 12335 0.3 1 11937 398 50 11283 0.3 1 10909 374 51 11635 0.3 1 11329 306 52 14441 0.3 1 13896 545 53 16596 0.3 1 16176 420 54 22796 0.3 1 22264 532 55 27245 0.3 1 26725 520 56 29034 0.3 1 28531 503 57 25175 0.3 1 24711 464 58 19249 0.3 1 18895 354 59 13199 0.3 1 12937 262 60 11331 0.3 1 11097 234 61 10422 0.3 1 10072 350 62 13099 0.3 1 12816 283 63 17286 0.3 1 16970 316 64 24318 0.3 1 23691 627 65 32289 0.3 1 31761 528 66 33754 0.3 1 33216 538 67 30830 0.3 1 30198 632 68 24075 0.3 1 23588 487 69 19455 0.3 1 19053 402 70 14533 0.3 1 14196 337 71 11538 0.3 1 11199 339 72 11917 0.3 1 11680 237 73 17304 0.3 1 16936 368 74 26663 0.3 1 26217 446 75 33656 0.3 1 33133 523 76 40102 0.3 1 39482 620 77 41088 0.3 1 40444 644 78 34600 0.3 1 34064 536 79 25374 0.3 1 24926 448 80 16882 0.3 1 16570 312 81 13385 0.3 1 13119 266 82 12004 0.3 1 11727 277 83 13839 0.3 1 13595 244 84 23484 0.3 1 23156 328 85 34674 0.3 1 33924 750 86 47845 0.3 1 47035 810 87 51994 0.3 1 51191 803 88 44239 0.3 1 43554 685 89 37151 0.3 1 36540 611 90 22351 0.3 1 21725 626 91 14787 0.3 1 14438 349 92 11917 0.3 1 11645 272 93 13226 0.3 1 13086 140 94 18148 0.3 1 17869 279 95 30304 0.3 1 29827 477 96 39426 0.3 1 38830 596 97 46343 0.3 1 45632 711 98 45725 0.3 1 44965 760 99 32747 0.3 1 32235 512 100 23466 0.3 1 23106 360 101 13512 0.3 1 13316 196 102 9606 0.3 1 9281 325 103 8048 0.3 1 7961 87 104 10038 0.3 1 9851 187 105 15415 0.3 1 15194 221 106 20284 0.3 1 19975 309 107 23256 0.3 1 22891 365 108 21865 0.3 1 21522 343 109 17852 0.3 1 17515 337 110 12388 0.3 1 12101 287 111 7216 0.3 1 7102 114 112 5000 0.3 1 4934 66 113 2441 0.3 1 2404 37 114 2539 0.3 1 2510 29 115 2086 0.3 1 2069 17 116 2143 0.3 1 2088 55 117 2314 0.3 1 2260 54 118 2157 0.3 1 2101 56 119 1229 0.3 1 1063 166 120 932 0.3 1 696 236 121 809 0.3 1 765 44 122 411 0.3 1 394 17 123 495 0.3 1 415 80 124 212 0.3 1 195 17 125 280 0.3 1 169 111 126 52 0.3 1 22 30 127 77 0.3 1 29 48 128 152 0.3 1 37 115 129 40 0.3 1 3 37 130 34 0.3 1 31 3 131 40 0.3 1 10 30 132 46 0.3 1 42 4 133 26 0.3 1 2 24 134 6 0.3 1 3 3 135 249 0.3 1 2 247 136 43 0.3 1 4 39 137 121 0.3 1 4 117 138 52 0.3 1 1 51 139 373 0.3 1 336 37 140 9 0.3 1 6 3 141 140 0.3 1 59 81 142 25 0.3 1 23 2 143 117 0.3 1 12 105 145 12 0.3 1 12 146 21 0.3 1 13 8 147 72 0.3 1 68 4 148 201 0.3 1 28 173 149 5 0.3 1 0 5 150 9 0.3 1 1 8 151 549 0.3 1 498 51 RUN STATISTICS FOR INPUT FILE: N1-H-iG-IgG_S10_L002_R2_001.fastq.gz ============================================= 5717719 sequences processed in total Sequences removed because they became shorter than the length cutoff of 20 bp: 2076 (0.0%)