# Basic configuration file for ONT Guppy basecaller software.

# Compatibility
compatible_flowcells                = FLO-MIN112
compatible_kits                     = SQK-LSK112,SQK-LSK112-XL,SQK-RAD112
compatible_kits_with_barcoding      = SQK-NBD112-24,SQK-NBD112-96,SQK-RBK112-24,SQK-RBK112-96

# Data trimming.
trim_strategy                       = dna
trim_threshold                      = 2.5
trim_min_events                     = 3

# Basecalling.
model_file                          = template_r10.4_e8.1_hac.jsn
chunk_size                          = 2000
gpu_runners_per_device              = 4
chunks_per_runner                   = 256
chunks_per_caller                   = 10000
overlap                             = 50
qscore_offset                       = -0.2891
qscore_scale                        = 1.032
builtin_scripts                     = 1
beam_width                          = 32
noisiest_section_scaling_max_size   = 8000

# Calibration strand detection
calib_reference                     = lambda_3.6kb.fasta
calib_min_sequence_length           = 3000
calib_max_sequence_length           = 3800
calib_min_coverage                  = 0.6

# Output.
records_per_fastq                   = 4000
min_qscore                          = 9.0

# Telemetry
ping_url                            = https://ping.oxfordnanoportal.com/basecall
ping_segment_duration               = 60