# Basic configuration file for ONT Guppy basecaller software.

# Data trimming.
trim_strategy                       = dna
trim_threshold                      = 2.5
trim_min_events                     = 3

# Basecalling.
model_file                          = template_r10.4.1_e8.2_260bps_fast.jsn
dorado_model_path                   = dna_r10.4.1_e8.2_260bps_fast@v4.1.0
chunk_size                          = 2000
num_callers                         = 1
gpu_runners_per_device              = 2
chunks_per_runner                   = 48
chunks_per_caller                   = 10000
overlap                             = 50
qscore_offset                       = -2.05
qscore_scale                        = 0.99
builtin_scripts                     = 1
beam_width                          = 32
noisiest_section_scaling_max_size   = 8000
use_quantile_scaling                = true

# IPC Settings.
ipc_threads                         = 1

# Barcoding Settings.
num_barcoding_buffers               = 8
num_reads_per_barcoding_buffer      = 1
num_mid_barcoding_buffers           = 20

# Calibration strand detection
calib_reference                     = lambda_3.6kb.fasta
calib_min_sequence_length           = 3000
calib_max_sequence_length           = 3800
calib_min_coverage                  = 0.6

# Output.
records_per_fastq                   = 4000
min_qscore                          = 8.0

# Telemetry
ping_url                            = https://ping.oxfordnanoportal.com/basecall
ping_segment_duration               = 60