******************************************************************************** STREME - Sensitive, Thorough, Rapid, Enriched Motif Elicitation ******************************************************************************** MEME version 5.4.0 (Release date: Mon Aug 16 16:18:19 2021 -0700) For further information on how to interpret these results please access https://meme-suite.org/meme. To get a copy of the MEME Suite software please access https://meme-suite.org. ******************************************************************************** ******************************************************************************** REFERENCE ******************************************************************************** If you use this program in your research, please cite: Timothy L. Bailey, "STREME: accurate and versatile sequence motif discovery", Bioinformatics, Mar. 24, 2021. ******************************************************************************** ******************************************************************************** ALPHABET "DNA with covalent modifications" DNA-LIKE ******************************************************************************** A "Adenine" 8510A8 ~ T "Thymine" A89610 C "Cytosine" A50026 ~ G "Guanine" 313695 c "5-Carboxylcytosine" FEE090 ~ 4 "Guanine:5-Carboxylcytosine" E0F3F8 f "5-Formylcytosine" FDAE61 ~ 3 "Guanine:5-Formylcytosine" ABD9E9 h "5-Hydroxymethylcytosine" F46D43 ~ 2 "Guanine:5-Hydroxymethylcytosine" 74ADD1 m "5-Methylcytosine" D73027 ~ 1 "Guanine:5-Methylcytosine" 4575B4 ? = ACGTcfhm1234 z = Ccfhm 9 = G1234 N = ACGT V = ACG H = ACT D = AGT B = CGT y = Ccf 8 = G34 M = AC R = AG W = AT S = CG Y = CT K = GT x = hm 7 = 12 ******************************************************************************** strands: + - Background letter frequencies A 0.24 C 0.244 G 0.244 T 0.24 c 4.3e-08 f 4.3e-08 h 0.000222 m 0.0156 1 0.0156 2 0.000222 3 4.3e-08 4 4.3e-08 MOTIF 1-1CCATCTTRKWW STREME-1 letter-probability matrix: alength= 12 w= 12 nsites= 222 E= 1.5e-010 0.167896 0.000184 0.278310 0.018064 0.000000 0.000000 0.000000 0.000012 0.530297 0.005236 0.000000 0.000000 0.000181 0.978485 0.000184 0.010653 0.000000 0.000000 0.000000 0.000012 0.010484 0.000000 0.000000 0.000000 0.005417 0.973249 0.000184 0.015889 0.000000 0.000000 0.000000 0.000012 0.005248 0.000000 0.000000 0.000000 0.999426 0.000184 0.000184 0.000181 0.000000 0.000000 0.000000 0.000012 0.000012 0.000000 0.000000 0.000000 0.000181 0.000184 0.000184 0.988954 0.000000 0.000000 0.000000 0.000012 0.010484 0.000000 0.000000 0.000000 0.015889 0.952305 0.000184 0.031598 0.000000 0.000000 0.000000 0.000012 0.000012 0.000000 0.000000 0.000000 0.000181 0.000184 0.000184 0.999426 0.000000 0.000000 0.000000 0.000012 0.000012 0.000000 0.000000 0.000000 0.000181 0.000184 0.057872 0.931266 0.000000 0.000000 0.000000 0.000012 0.010484 0.000000 0.000000 0.000000 0.336210 0.000184 0.595318 0.068263 0.000000 0.000000 0.000000 0.000012 0.000012 0.000000 0.000000 0.000000 0.005417 0.031620 0.409759 0.547944 0.000000 0.000000 0.000000 0.000012 0.005248 0.000000 0.000000 0.000000 0.516520 0.005421 0.120655 0.352144 0.000000 0.000000 0.000000 0.000012 0.005248 0.000000 0.000000 0.000000 0.568900 0.010657 0.031612 0.388808 0.000000 0.000000 0.000000 0.000012 0.000012 0.000000 0.000000 0.000000 MOTIF 2-AAKAWAWAD STREME-2 letter-probability matrix: alength= 12 w= 9 nsites= 1040 E= 8.3e-010 0.882351 0.000084 0.000084 0.117471 0.000000 0.000000 0.000000 0.000005 0.000005 0.000000 0.000000 0.000000 0.992599 0.000084 0.000084 0.002462 0.000000 0.000000 0.000000 0.000005 0.004765 0.000000 0.000000 0.000000 0.063218 0.082321 0.358054 0.496396 0.000000 0.000000 0.000000 0.000005 0.000005 0.000000 0.000000 0.000000 0.987102 0.000084 0.000084 0.003199 0.000000 0.000000 0.000000 0.000005 0.009525 0.000000 0.000000 0.000000 0.607711 0.000084 0.000084 0.392110 0.000000 0.000000 0.000000 0.000005 0.000005 0.000000 0.000000 0.000000 0.714681 0.000084 0.255787 0.029437 0.000000 0.000000 0.000000 0.000005 0.000005 0.000000 0.000000 0.000000 0.549554 0.000084 0.000084 0.447887 0.000000 0.000000 0.000000 0.000005 0.002385 0.000000 0.000000 0.000000 0.872296 0.000084 0.000084 0.127525 0.000000 0.000000 0.000000 0.000005 0.000005 0.000000 0.000000 0.000000 0.425626 0.059751 0.255518 0.225772 0.000000 0.000000 0.000000 0.030948 0.002385 0.000000 0.000000 0.000000 ******************************************************************************** Stopped because maximum number of motifs (2) reached. ******************************************************************************** COMMAND: streme -p common/dna_with_covalent.fa -oc results/streme8 -objfun de -order 1 -nmotifs 2 -alph common/dna_with_covalent.alph -nmotifs 2 -hofract 0.2 -verbosity 1 ******************************************************************************** CPU: Timothys-Mac-Mini.local ******************************************************************************** FINALTIME: 24.03 seconds ********************************************************************************