# from Miten: # I downloaded the fastq file from https://osf.io/8f6n9/ # went to folder 20200302.... and download file IVT1.fastq.zst # extract and replace Us with Ts in the fastq (!) # align to genome with minimap2 -ax map-ont # Make bam using samtools # I got this BAM file from Miten: wget http://public.gi.ucsc.edu/~miten/covid-19/IVT.sorted.bam wget http://public.gi.ucsc.edu/~miten/covid-19/IVT.sorted.bam.bai # sigh... need to change chrom name for UCSC samtools view -h IVT.sorted.bam | sed -e 's/NC_045512.2/NC_045512v2/' | samtools view -Sb > IVT.ucsc.s.bam samtools index IVT.ucsc.s.bam bamCoverage -b IVT.ucsc.s.bam -o IVT.bw --binSize=1 -p 8 ln -s `pwd`/IVT.bw /gbdb/wuhCor1/bbi/IVT.bw