>universalKitMINTadapter5prime | MINT-Universal cDNA synthesis kit (Cat.#SK002) | At the 5'end is added: 5'-AAGCAGTGGTATCAACGCAGAGTACGGGGG-3' : NOTE: Only upper- and lower-case A, C, G, T, and N characters should be used in Newbler trimming. Do not use X or N as masking characters.
AAGCAGTGGTATCAACGCAGAGTACGGGGG

>universalKitMINTadapter3primeAA | At the 3' end: 5'-AAGCAGTGGTATCAACGCAGAGTAC(T)30VN-3, in runAssembly N=low-quality base, so give all possibilities for N, (T)30 & V: first replace N with A
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA
>universalKitMINTadapter3primeCA
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCA
>universalKitMINTadapter3primeGA
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGA

>universalKitMINTadapter3primeAT | At the 3' end: 5'-AAGCAGTGGTATCAACGCAGAGTAC(T)30VN-3; replace 'N' with 'T' 
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAT
>universalKitMINTadapter3primeCT
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCT
>universalKitMINTadapter3primeGT
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT

>universalKitMINTadapter3primeAG | At the 3' end: 5'-AAGCAGTGGTATCAACGCAGAGTAC(T)30VN-3; replace 'N' with 'G' 
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAG
>universalKitMINTadapter3primeCG
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCG
>universalKitMINTadapter3primeGG
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG

>universalKitMINTadapter3primeAC | At the 3' end: 5'-AAGCAGTGGTATCAACGCAGAGTAC(T)30VN-3; replace 'N' with 'C' 
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAC
>universalKitMINTadapter3primeCC
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC
>universalKitMINTadapter3primeGC
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC


>newGsuIMINT_AA | 3' GsuI MINT (5'-AAGCAGTGGTATCAACGCAGAGTCTGGAG(T)22VN-3'); replace 'N' with 'A'.  (Note: the newSMART 5' adapter is same as the above 'universalKitMINTadapter5prime')
AAGCAGTGGTATCAACGCAGAGTCTGGAGTTTTTTTTTTTTTTTTTTTTTTAA
>newGsuIMINT_CA | 3' GsuI MINT (5'-AAGCAGTGGTATCAACGCAGAGTCTGGAG(T)22VN-3')
AAGCAGTGGTATCAACGCAGAGTCTGGAGTTTTTTTTTTTTTTTTTTTTTTCA
>newGsuIMINT_GA | 3' GsuI MINT (5'-AAGCAGTGGTATCAACGCAGAGTCTGGAG(T)22VN-3')
AAGCAGTGGTATCAACGCAGAGTCTGGAGTTTTTTTTTTTTTTTTTTTTTTGA

>newGsuIMINT_AT | 3' GsuI MINT (5'-AAGCAGTGGTATCAACGCAGAGTCTGGAG(T)22VN-3'); replace 'N' with 'T'
AAGCAGTGGTATCAACGCAGAGTCTGGAGTTTTTTTTTTTTTTTTTTTTTTAT
>newGsuIMINT_CT | 3' GsuI MINT (5'-AAGCAGTGGTATCAACGCAGAGTCTGGAG(T)22VN-3')
AAGCAGTGGTATCAACGCAGAGTCTGGAGTTTTTTTTTTTTTTTTTTTTTTCT
>newGsuIMINT_GT | 3' GsuI MINT (5'-AAGCAGTGGTATCAACGCAGAGTCTGGAG(T)22VN-3')
AAGCAGTGGTATCAACGCAGAGTCTGGAGTTTTTTTTTTTTTTTTTTTTTTGT

>newGsuIMINT_AG | 3' GsuI MINT (5'-AAGCAGTGGTATCAACGCAGAGTCTGGAG(T)22VN-3'); replace 'N' with 'G'
AAGCAGTGGTATCAACGCAGAGTCTGGAGTTTTTTTTTTTTTTTTTTTTTTAG
>newGsuIMINT_CG | 3' GsuI MINT (5'-AAGCAGTGGTATCAACGCAGAGTCTGGAG(T)22VN-3')
AAGCAGTGGTATCAACGCAGAGTCTGGAGTTTTTTTTTTTTTTTTTTTTTTCG
>newGsuIMINT_GG | 3' GsuI MINT (5'-AAGCAGTGGTATCAACGCAGAGTCTGGAG(T)22VN-3')
AAGCAGTGGTATCAACGCAGAGTCTGGAGTTTTTTTTTTTTTTTTTTTTTTGG

>newGsuIMINT_AC | 3' GsuI MINT (5'-AAGCAGTGGTATCAACGCAGAGTCTGGAG(T)22VN-3'); replace 'N' with 'C'
AAGCAGTGGTATCAACGCAGAGTCTGGAGTTTTTTTTTTTTTTTTTTTTTTAC
>newGsuIMINT_CC | 3' GsuI MINT (5'-AAGCAGTGGTATCAACGCAGAGTCTGGAG(T)22VN-3')
AAGCAGTGGTATCAACGCAGAGTCTGGAGTTTTTTTTTTTTTTTTTTTTTTCC
>newGsuIMINT_GC | 3' GsuI MINT (5'-AAGCAGTGGTATCAACGCAGAGTCTGGAG(T)22VN-3')
AAGCAGTGGTATCAACGCAGAGTCTGGAGTTTTTTTTTTTTTTTTTTTTTTGC


>oldSMARTadapter5prime | From Evrogen's cDNA synthesis service (Cat.#CS030): For non-directional cDNA synthesis (old pre Aug 2009). | At the 5'end is added: SMART Oligo II: 5'-AAGCAGTGGTATCAACGCAGAGTACGCGGG-3' NOTE: At the 4th position from the 3' end, the C replaces a G. 
AAGCAGTGGTATCAACGCAGAGTACGCGGG

>oldSMARTadapter3primeAA | At the 3' end:  CDS primer 5'-AAGCAGTGGTATCAACGCAGAGTA-d(T)20VN -3';  In runAssembly N=low-quality base, so give all possibilities for N, (T)30 & V: first replace N with A
AAGCAGTGGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTAA
>oldSMARTadapter3primeCA
AAGCAGTGGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTCA
>oldSMARTadapter3primeGA
AAGCAGTGGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTGA

>oldSMARTadapter3primeAT
AAGCAGTGGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTAT
>oldSMARTadapter3primeCT
AAGCAGTGGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTCT
>oldSMARTadapter3primeGT
AAGCAGTGGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTGT

>oldSMARTadapter3primeAC
AAGCAGTGGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTAC
>oldSMARTadapter3primeCC
AAGCAGTGGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTCC
>oldSMARTadapter3primeGC
AAGCAGTGGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTGC

>oldSMARTadapter3primeAG
AAGCAGTGGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTAG
>oldSMARTadapter3primeCG
AAGCAGTGGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTCG
>oldSMARTadapter3primeGG
AAGCAGTGGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTGG


>newSMARTadapter5prime | From Evrogen's cDNA synthesis service (Cat.#CS030) : For non-directional cDNA syntehis (new - August 2009 on): | At the 5'end is added: SMART Oligo II: 5'-AAGCAGTGGTATCAACGCAGAGTACGCGGG-3'
AAGCAGTGGTATCAACGCAGAGTACGCGGG

>newSMARTadapter3primeAA | SmartGsu T20 5'-AAGCAGTGGTATCAACGCAGAGTACTGGAGd(T)20VN-3';  In runAssembly N=low-quality base, so give all possibilities for N, (T)30 & V: first replace N with A
AAGCAGTGGTATCAACGCAGAGTACTGGAGTTTTTTTTTTTTTTTTTTTTAA
>newSMARTadapter3primeCA
AAGCAGTGGTATCAACGCAGAGTACTGGAGTTTTTTTTTTTTTTTTTTTTCA
>SMARTadapter3primeGA
AAGCAGTGGTATCAACGCAGAGTACTGGAGTTTTTTTTTTTTTTTTTTTTGA

>newSMARTadapter3primeAT
AAGCAGTGGTATCAACGCAGAGTACTGGAGTTTTTTTTTTTTTTTTTTTTAT
>newSMARTadapter3primeCT
AAGCAGTGGTATCAACGCAGAGTACTGGAGTTTTTTTTTTTTTTTTTTTTCT
>SMARTadapter3primeGT
AAGCAGTGGTATCAACGCAGAGTACTGGAGTTTTTTTTTTTTTTTTTTTTGT

>newSMARTadapter3primeAC
AAGCAGTGGTATCAACGCAGAGTACTGGAGTTTTTTTTTTTTTTTTTTTTAC
>newSMARTadapter3primeCC
AAGCAGTGGTATCAACGCAGAGTACTGGAGTTTTTTTTTTTTTTTTTTTTCC
>newSMARTadapter3primeGC
AAGCAGTGGTATCAACGCAGAGTACTGGAGTTTTTTTTTTTTTTTTTTTTGC

>newSMARTadapter3primeAG
AAGCAGTGGTATCAACGCAGAGTACTGGAGTTTTTTTTTTTTTTTTTTTTAG
>newSMARTadapter3primeCG
AAGCAGTGGTATCAACGCAGAGTACTGGAGTTTTTTTTTTTTTTTTTTTTCG
>newSMARTadapter3primeGG
AAGCAGTGGTATCAACGCAGAGTACTGGAGTTTTTTTTTTTTTTTTTTTTGG


>Roche_Forward_primer_and_StdKey (Primer A-Key + TCAG Standard Library Key):  5'-CGTATCGCCTCCCTCGCGCCA-{TCAG}-{MID}-{template-specific-sequence}-3'
CGTATCGCCTCCCTCGCGCCATCAG
>Roche_Reverse_primer_and_StdKey (Primer B-Key + TCAG Standard Library Key):   5'-CTATGCGCCTTGCCAGCCCGC-{TCAG}-{MID}-{template-specific-sequence}-3-3'
CTATGCGCCTTGCCAGCCCGCTCAG

>Roche_Forward_primer_and_RapidLibKey (Primer A-Key + GACT Rapid Library Key):  5'-CGTATCGCCTCCCTCGCGCCA-{GACT}-{MID}-{template-specific-sequence}-3'
CGTATCGCCTCCCTCGCGCCAGACT
>Roche_Reverse_primer_and_RapidLibKey (Primer B-Key + GACT Rapid Library Key):   5'-CTATGCGCCTTGCCAGCCCGC-{GACT}-{MID}-{template-specific-sequence}-3'
CTATGCGCCTTGCCAGCCCGCGACT


>emPCRprimerA   5'-CCATCTCATCCCTGCGTGTC-3' (Roche_Rapid_Library Primer B), added here May 2011,  From:http://seqanswers.com/forums/showthread.php?t=4819
CCATCTCATCCCTGCGTGTC
>TIadaptorA     5'-CCATCTCATCCCTGCGTGTCTCCGACTCAG-3'
CCATCTCATCCCTGCGTGTCTCCGACTCAG

>emPCRprimerB   5'-CCTATCCCCTGTGTGCCTTG-3' (Roche_Rapid_Library Primer B)
CCTATCCCCTGTGTGCCTTG
>TIadaptorB     5'-CCTATCCCCTGTGTGCCTTGGCAGTCTCAG-3'
CCTATCCCCTGTGTGCCTTGGCAGTCTCAG
