[M::bwa_idx_load_from_disk] read 0 ALT contigs [M::process] read 1982188 sequences (200000111 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12342, 714904, 565, 657) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 137, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (150.36, 89.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 605) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 240, 344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 690) [M::mem_pestat] mean and std.dev: (267.33, 126.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 863) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1818, 3660, 5920) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14124) [M::mem_pestat] mean and std.dev: (4048.74, 2623.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18226) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1026, 2934, 5563) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14637) [M::mem_pestat] mean and std.dev: (3543.85, 2840.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19174) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1982188 reads in 205.269 CPU sec, 29.197 real sec [M::process] read 1982206 sequences (200000195 bp)... [M::process] read 1982244 sequences (200000061 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12317, 715283, 604, 650) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 133, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 462) [M::mem_pestat] mean and std.dev: (148.20, 87.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 588) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 239, 342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 684) [M::mem_pestat] mean and std.dev: (265.88, 125.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 855) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1828, 3640, 6035) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14449) [M::mem_pestat] mean and std.dev: (4110.31, 2665.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18656) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1204, 3072, 5643) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14521) [M::mem_pestat] mean and std.dev: (3615.57, 2810.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18960) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1982206 reads in 200.257 CPU sec, 12.763 real sec [M::process] read 1982148 sequences (200000083 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13310, 714668, 641, 668) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 132, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 453) [M::mem_pestat] mean and std.dev: (146.26, 85.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 576) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 237, 339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 677) [M::mem_pestat] mean and std.dev: (263.61, 123.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 846) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1812, 3591, 6066) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14574) [M::mem_pestat] mean and std.dev: (4008.85, 2659.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18828) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1118, 2812, 5514) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14306) [M::mem_pestat] mean and std.dev: (3495.48, 2792.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18702) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1982244 reads in 202.513 CPU sec, 13.111 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13549, 714394, 600, 652) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 133, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 465) [M::mem_pestat] mean and std.dev: (148.46, 88.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 592) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 239, 343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 687) [M::mem_pestat] mean and std.dev: (266.36, 125.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 859) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1772, 3650, 6247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15197) [M::mem_pestat] mean and std.dev: (4077.66, 2734.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19672) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1164, 2973, 5975) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15597) [M::mem_pestat] mean and std.dev: (3642.83, 2871.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20408) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1982148 reads in 203.243 CPU sec, 12.920 real sec [M::process] read 1982418 sequences (200000011 bp)... [M::process] read 1982480 sequences (200000119 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14127, 714117, 577, 666) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 131, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 441) [M::mem_pestat] mean and std.dev: (145.54, 84.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 560) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 237, 336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668) [M::mem_pestat] mean and std.dev: (262.19, 121.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 834) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1690, 3324, 5658) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13594) [M::mem_pestat] mean and std.dev: (3826.75, 2667.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17562) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (949, 2736, 5829) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15589) [M::mem_pestat] mean and std.dev: (3494.03, 2936.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20469) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1982418 reads in 204.491 CPU sec, 13.016 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12517, 715953, 570, 717) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 133, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 456) [M::mem_pestat] mean and std.dev: (146.54, 86.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 580) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 236, 336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668) [M::mem_pestat] mean and std.dev: (262.28, 121.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 834) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1862, 3780, 6429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15563) [M::mem_pestat] mean and std.dev: (4192.54, 2731.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20130) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1405, 3079, 5691) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14263) [M::mem_pestat] mean and std.dev: (3672.73, 2739.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18549) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1982336 sequences (200000039 bp)... [M::mem_process_seqs] Processed 1982480 reads in 205.104 CPU sec, 13.048 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11537, 717085, 585, 596) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 135, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (148.61, 88.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 601) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 237, 340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 680) [M::mem_pestat] mean and std.dev: (264.11, 124.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 850) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1678, 3369, 6034) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14746) [M::mem_pestat] mean and std.dev: (3969.90, 2724.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19102) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1240, 3089, 5530) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14110) [M::mem_pestat] mean and std.dev: (3569.64, 2734.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18400) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1982390 sequences (200000131 bp)... [M::mem_process_seqs] Processed 1982336 reads in 203.114 CPU sec, 12.922 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12409, 715623, 595, 665) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 133, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 462) [M::mem_pestat] mean and std.dev: (147.07, 86.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 588) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 239, 343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 687) [M::mem_pestat] mean and std.dev: (266.28, 125.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 859) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1926, 3874, 6430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15438) [M::mem_pestat] mean and std.dev: (4269.53, 2687.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19942) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1209, 3094, 5611) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14415) [M::mem_pestat] mean and std.dev: (3576.84, 2794.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18817) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1982386 sequences (200000100 bp)... [M::mem_process_seqs] Processed 1982390 reads in 203.774 CPU sec, 12.974 real sec [M::process] read 1982442 sequences (200000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13218, 714816, 573, 672) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 131, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 456) [M::mem_pestat] mean and std.dev: (146.30, 86.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 580) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 238, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 683) [M::mem_pestat] mean and std.dev: (264.89, 124.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 854) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1853, 3566, 6304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15206) [M::mem_pestat] mean and std.dev: (4176.91, 2797.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19657) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1168, 2902, 5436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13972) [M::mem_pestat] mean and std.dev: (3484.20, 2768.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18240) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1982386 reads in 203.393 CPU sec, 12.989 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13809, 715460, 612, 686) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 130, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 440) [M::mem_pestat] mean and std.dev: (144.15, 83.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 559) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 235, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664) [M::mem_pestat] mean and std.dev: (260.56, 120.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 829) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1667, 3609, 6042) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14792) [M::mem_pestat] mean and std.dev: (4001.05, 2669.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19167) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1413, 3151, 5866) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14772) [M::mem_pestat] mean and std.dev: (3739.09, 2793.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19225) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1982434 sequences (200000092 bp)... [M::mem_process_seqs] Processed 1982442 reads in 204.834 CPU sec, 13.010 real sec [M::process] read 1982250 sequences (200000188 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14121, 715091, 578, 697) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 129, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 431) [M::mem_pestat] mean and std.dev: (141.78, 81.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 547) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 234, 333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663) [M::mem_pestat] mean and std.dev: (259.74, 120.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 828) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1829, 3601, 6036) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14450) [M::mem_pestat] mean and std.dev: (4079.99, 2694.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18657) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1014, 2970, 5852) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15528) [M::mem_pestat] mean and std.dev: (3570.50, 2847.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20366) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1982434 reads in 205.812 CPU sec, 13.140 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13862, 713255, 606, 694) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 130, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 451) [M::mem_pestat] mean and std.dev: (144.34, 85.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 574) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 236, 339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679) [M::mem_pestat] mean and std.dev: (263.57, 124.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 849) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1716, 3469, 6014) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14610) [M::mem_pestat] mean and std.dev: (4060.37, 2668.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18908) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1023, 2862, 5768) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15258) [M::mem_pestat] mean and std.dev: (3579.57, 2926.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20003) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1982300 sequences (200000031 bp)... [M::mem_process_seqs] Processed 1982250 reads in 205.088 CPU sec, 13.021 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13320, 714792, 586, 744) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 132, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 452) [M::mem_pestat] mean and std.dev: (145.50, 85.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 575) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 237, 339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679) [M::mem_pestat] mean and std.dev: (263.67, 124.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 849) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1841, 3980, 6317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15269) [M::mem_pestat] mean and std.dev: (4299.45, 2779.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19745) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1164, 2916, 5755) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14937) [M::mem_pestat] mean and std.dev: (3603.87, 2848.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19528) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1982590 sequences (200000179 bp)... [M::mem_process_seqs] Processed 1982300 reads in 206.341 CPU sec, 13.134 real sec [M::process] read 1982530 sequences (200000038 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13270, 715090, 614, 698) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 130, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 447) [M::mem_pestat] mean and std.dev: (142.86, 83.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 569) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 234, 333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663) [M::mem_pestat] mean and std.dev: (260.02, 120.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 828) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1649, 3502, 5869) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14309) [M::mem_pestat] mean and std.dev: (3966.42, 2677.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18529) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1092, 2839, 5435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14121) [M::mem_pestat] mean and std.dev: (3440.14, 2751.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18464) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1982590 reads in 208.567 CPU sec, 13.313 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11916, 715223, 609, 628) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 133, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 467) [M::mem_pestat] mean and std.dev: (147.90, 87.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 595) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 238, 340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 680) [M::mem_pestat] mean and std.dev: (264.52, 124.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 850) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1946, 3785, 6522) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15674) [M::mem_pestat] mean and std.dev: (4264.66, 2814.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20250) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1129, 3052, 5716) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14890) [M::mem_pestat] mean and std.dev: (3621.93, 2824.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19477) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1982530 reads in 208.250 CPU sec, 13.230 real sec [M::process] read 1982276 sequences (200000106 bp)... [M::process] read 1982204 sequences (200000165 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11164, 715694, 623, 721) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 136, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470) [M::mem_pestat] mean and std.dev: (150.05, 88.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 238, 339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675) [M::mem_pestat] mean and std.dev: (264.12, 123.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 843) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1720, 3518, 6127) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14941) [M::mem_pestat] mean and std.dev: (4033.55, 2723.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19348) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1304, 3209, 6223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16061) [M::mem_pestat] mean and std.dev: (3858.48, 2900.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20980) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1982276 reads in 205.158 CPU sec, 13.096 real sec [M::process] read 1982220 sequences (200000055 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11413, 716222, 594, 647) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 135, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (149.80, 89.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 613) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 238, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 681) [M::mem_pestat] mean and std.dev: (265.10, 124.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1687, 3732, 6097) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14917) [M::mem_pestat] mean and std.dev: (4094.28, 2693.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19327) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1054, 3165, 5832) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15388) [M::mem_pestat] mean and std.dev: (3715.55, 2890.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20166) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1982204 reads in 203.930 CPU sec, 12.989 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11608, 715359, 582, 673) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 135, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (150.10, 90.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 609) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 238, 340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678) [M::mem_pestat] mean and std.dev: (264.91, 123.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1869, 3763, 6195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14847) [M::mem_pestat] mean and std.dev: (4224.31, 2749.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19173) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1152, 2820, 5889) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15363) [M::mem_pestat] mean and std.dev: (3616.12, 2867.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20100) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1982220 reads in 204.570 CPU sec, 12.978 real sec [M::process] read 1982146 sequences (200000129 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12066, 716495, 590, 648) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 134, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 466) [M::mem_pestat] mean and std.dev: (148.17, 87.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::process] read 1982258 sequences (200000118 bp)... [M::mem_pestat] (25, 50, 75) percentile: (171, 239, 342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 684) [M::mem_pestat] mean and std.dev: (265.82, 124.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 855) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1928, 3933, 6253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14903) [M::mem_pestat] mean and std.dev: (4213.36, 2609.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19228) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1184, 3022, 5724) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14804) [M::mem_pestat] mean and std.dev: (3591.85, 2792.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19344) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1982146 reads in 204.113 CPU sec, 12.994 real sec [M::process] read 1982316 sequences (200000166 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11533, 716117, 574, 673) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 134, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 465) [M::mem_pestat] mean and std.dev: (147.73, 87.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 592) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 238, 339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 677) [M::mem_pestat] mean and std.dev: (264.37, 123.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 846) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1866, 3694, 6034) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14370) [M::mem_pestat] mean and std.dev: (4074.30, 2596.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18538) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1096, 2984, 5807) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15229) [M::mem_pestat] mean and std.dev: (3607.56, 2856.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19940) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1982258 reads in 203.508 CPU sec, 12.994 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10117, 717959, 612, 648) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 133, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469) [M::mem_pestat] mean and std.dev: (148.22, 88.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 597) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 238, 340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 680) [M::mem_pestat] mean and std.dev: (264.35, 124.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 850) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1762, 3597, 6023) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14545) [M::mem_pestat] mean and std.dev: (4018.35, 2688.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18806) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1019, 2981, 5488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14426) [M::mem_pestat] mean and std.dev: (3492.93, 2806.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18895) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1982310 sequences (200000049 bp)... [M::mem_process_seqs] Processed 1982316 reads in 203.650 CPU sec, 12.915 real sec [M::process] read 1982320 sequences (200000044 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9966, 718032, 533, 654) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 138, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 476) [M::mem_pestat] mean and std.dev: (149.76, 87.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 238, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 683) [M::mem_pestat] mean and std.dev: (265.12, 124.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 854) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1942, 3894, 6256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14884) [M::mem_pestat] mean and std.dev: (4163.05, 2651.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19198) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1266, 2696, 5750) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14718) [M::mem_pestat] mean and std.dev: (3534.81, 2790.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19202) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1982310 reads in 202.833 CPU sec, 12.959 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10918, 718160, 569, 679) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 135, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (149.25, 88.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 613) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 238, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 683) [M::mem_pestat] mean and std.dev: (265.02, 124.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 854) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1774, 3594, 6205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15067) [M::mem_pestat] mean and std.dev: (4070.77, 2667.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19498) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1121, 3006, 5675) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14783) [M::mem_pestat] mean and std.dev: (3634.59, 2889.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19337) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1982300 sequences (200000061 bp)... [M::mem_process_seqs] Processed 1982320 reads in 203.048 CPU sec, 12.880 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12037, 715976, 585, 658) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 133, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 453) [M::mem_pestat] mean and std.dev: (144.87, 83.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 576) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 237, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674) [M::mem_pestat] mean and std.dev: (263.50, 123.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 842) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1701, 3695, 6268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15402) [M::mem_pestat] mean and std.dev: (4134.44, 2761.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19969) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1173, 3299, 5996) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15642) [M::mem_pestat] mean and std.dev: (3752.28, 2930.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20465) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1982474 sequences (200000019 bp)... [M::mem_process_seqs] Processed 1982300 reads in 204.403 CPU sec, 13.000 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13162, 715599, 576, 666) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 129, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 433) [M::mem_pestat] mean and std.dev: (141.72, 81.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 550) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 235, 333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661) [M::mem_pestat] mean and std.dev: (260.08, 119.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 825) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1704, 3484, 5946) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14430) [M::mem_pestat] mean and std.dev: (3922.01, 2628.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18672) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1239, 3103, 5655) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14487) [M::mem_pestat] mean and std.dev: (3602.04, 2788.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18903) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1982248 sequences (200000189 bp)... [M::mem_process_seqs] Processed 1982474 reads in 206.073 CPU sec, 13.110 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12383, 714960, 618, 696) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 132, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 458) [M::mem_pestat] mean and std.dev: (145.04, 85.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 583) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 237, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674) [M::mem_pestat] mean and std.dev: (263.14, 123.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 842) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1594, 3718, 6396) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16000) [M::mem_pestat] mean and std.dev: (4109.12, 2802.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20802) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1084, 2897, 5348) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13876) [M::mem_pestat] mean and std.dev: (3422.25, 2684.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18140) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1982274 sequences (200000027 bp)... [M::mem_process_seqs] Processed 1982248 reads in 204.905 CPU sec, 13.040 real sec [M::process] read 1982458 sequences (200000030 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11968, 716173, 584, 641) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 133, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 454) [M::mem_pestat] mean and std.dev: (145.97, 85.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 577) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 237, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676) [M::mem_pestat] mean and std.dev: (263.18, 123.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 845) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1715, 3535, 5963) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14459) [M::mem_pestat] mean and std.dev: (4000.98, 2695.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18707) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1107, 2871, 5838) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15300) [M::mem_pestat] mean and std.dev: (3588.25, 2905.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20031) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1982274 reads in 205.901 CPU sec, 13.143 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11953, 716761, 559, 644) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 131, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 449) [M::mem_pestat] mean and std.dev: (143.83, 83.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 571) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 236, 336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670) [M::mem_pestat] mean and std.dev: (261.92, 121.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 837) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1801, 3443, 6085) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14653) [M::mem_pestat] mean and std.dev: (4013.10, 2737.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18937) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1239, 3133, 5508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14046) [M::mem_pestat] mean and std.dev: (3606.01, 2720.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18315) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1982454 sequences (200000066 bp)... [M::mem_process_seqs] Processed 1982458 reads in 207.012 CPU sec, 13.121 real sec [M::process] read 1982436 sequences (200000122 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11337, 716384, 581, 708) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 133, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 455) [M::mem_pestat] mean and std.dev: (144.57, 85.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 579) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 237, 339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 677) [M::mem_pestat] mean and std.dev: (263.55, 124.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 846) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (2013, 3737, 6354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15036) [M::mem_pestat] mean and std.dev: (4213.47, 2734.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19377) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1112, 2745, 5487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14237) [M::mem_pestat] mean and std.dev: (3425.59, 2777.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18612) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1982454 reads in 206.461 CPU sec, 13.213 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9848, 717681, 653, 658) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 133, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (148.47, 88.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 605) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 237, 337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671) [M::mem_pestat] mean and std.dev: (262.59, 122.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 838) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1620, 3247, 5873) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14379) [M::mem_pestat] mean and std.dev: (3896.53, 2709.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18632) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1268, 3313, 5995) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15449) [M::mem_pestat] mean and std.dev: (3786.74, 2877.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20176) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1982290 sequences (200000031 bp)... [M::mem_process_seqs] Processed 1982436 reads in 205.208 CPU sec, 13.017 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10391, 717006, 579, 674) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 136, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 479) [M::mem_pestat] mean and std.dev: (150.11, 90.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 610) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 237, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674) [M::mem_pestat] mean and std.dev: (263.11, 123.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 842) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1908, 3759, 6138) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14598) [M::mem_pestat] mean and std.dev: (4095.99, 2626.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18828) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1116, 2616, 5524) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14340) [M::mem_pestat] mean and std.dev: (3443.34, 2814.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18748) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1982242 sequences (200000075 bp)... [M::mem_process_seqs] Processed 1982290 reads in 203.869 CPU sec, 12.973 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10666, 717934, 588, 717) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 136, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473) [M::mem_pestat] mean and std.dev: (148.68, 87.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::process] read 1982322 sequences (200000027 bp)... [M::mem_pestat] (25, 50, 75) percentile: (170, 237, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674) [M::mem_pestat] mean and std.dev: (263.48, 123.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 842) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1758, 3529, 5892) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14160) [M::mem_pestat] mean and std.dev: (4017.04, 2700.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18294) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1186, 3259, 5727) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14809) [M::mem_pestat] mean and std.dev: (3677.45, 2797.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19350) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1982242 reads in 203.460 CPU sec, 12.958 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11047, 716686, 569, 710) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 134, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 467) [M::mem_pestat] mean and std.dev: (148.11, 86.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 236, 335) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667) [M::mem_pestat] mean and std.dev: (261.63, 121.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 833) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1742, 3966, 6127) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14897) [M::mem_pestat] mean and std.dev: (4223.85, 2777.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19282) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1172, 2994, 5794) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15038) [M::mem_pestat] mean and std.dev: (3648.47, 2853.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19660) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1982566 sequences (200000161 bp)... [M::mem_process_seqs] Processed 1982322 reads in 203.990 CPU sec, 12.976 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12056, 716574, 569, 624) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 133, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 448) [M::mem_pestat] mean and std.dev: (145.62, 83.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 569) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 233, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654) [M::mem_pestat] mean and std.dev: (258.03, 117.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 816) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1816, 3626, 5983) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14317) [M::mem_pestat] mean and std.dev: (4063.19, 2705.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18484) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1205, 3379, 6277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16421) [M::mem_pestat] mean and std.dev: (3858.50, 2961.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21493) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1983042 sequences (200000074 bp)... [M::mem_process_seqs] Processed 1982566 reads in 206.092 CPU sec, 13.112 real sec [M::process] read 1982372 sequences (200000152 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12206, 718217, 552, 599) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 129, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 423) [M::mem_pestat] mean and std.dev: (140.77, 78.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 536) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 228, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619) [M::mem_pestat] mean and std.dev: (249.77, 109.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1650, 3557, 5950) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14550) [M::mem_pestat] mean and std.dev: (3956.99, 2688.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18850) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1156, 2988, 5771) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15001) [M::mem_pestat] mean and std.dev: (3551.62, 2791.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19616) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1983042 reads in 210.389 CPU sec, 13.431 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9908, 717763, 573, 670) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 135, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469) [M::mem_pestat] mean and std.dev: (148.69, 88.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 597) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 237, 340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 680) [M::mem_pestat] mean and std.dev: (264.22, 124.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 850) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1909, 3630, 5679) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13219) [M::mem_pestat] mean and std.dev: (3952.97, 2511.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16989) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1124, 2965, 5405) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13967) [M::mem_pestat] mean and std.dev: (3495.56, 2776.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18248) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1982256 sequences (200000096 bp)... [M::mem_process_seqs] Processed 1982372 reads in 202.953 CPU sec, 12.862 real sec [M::process] read 1982314 sequences (200000085 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10057, 717696, 611, 694) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 136, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 479) [M::mem_pestat] mean and std.dev: (150.10, 90.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 610) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 237, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 683) [M::mem_pestat] mean and std.dev: (264.38, 125.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 854) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1931, 3694, 6269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14945) [M::mem_pestat] mean and std.dev: (4136.01, 2656.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19283) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1144, 3225, 5779) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15049) [M::mem_pestat] mean and std.dev: (3662.30, 2792.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19684) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1982256 reads in 202.662 CPU sec, 12.946 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10195, 716766, 577, 661) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 137, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 485) [M::mem_pestat] mean and std.dev: (151.24, 90.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 618) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 237, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 683) [M::mem_pestat] mean and std.dev: (264.45, 125.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 854) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1691, 3579, 5899) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14315) [M::mem_pestat] mean and std.dev: (3984.42, 2730.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18523) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1118, 3110, 5844) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15296) [M::mem_pestat] mean and std.dev: (3690.19, 2915.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20022) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1982294 sequences (200000032 bp)... [M::mem_process_seqs] Processed 1982314 reads in 203.893 CPU sec, 12.942 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10595, 716721, 572, 643) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 136, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (150.10, 90.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 613) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 237, 340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682) [M::mem_pestat] mean and std.dev: (264.11, 125.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 853) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1852, 3504, 6022) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14362) [M::mem_pestat] mean and std.dev: (4068.63, 2705.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18532) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1375, 2995, 5456) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13618) [M::mem_pestat] mean and std.dev: (3555.90, 2688.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17699) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1982194 sequences (200000106 bp)... [M::mem_process_seqs] Processed 1982294 reads in 204.070 CPU sec, 12.984 real sec [M::process] read 1982120 sequences (200000197 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10735, 716977, 628, 623) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 133, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (148.57, 89.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 604) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 237, 340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682) [M::mem_pestat] mean and std.dev: (263.96, 125.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 853) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1890, 3467, 6357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15291) [M::mem_pestat] mean and std.dev: (4130.70, 2761.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19758) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1142, 3123, 5653) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14675) [M::mem_pestat] mean and std.dev: (3584.00, 2804.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19186) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1982194 reads in 202.831 CPU sec, 12.905 real sec [M::process] read 1982240 sequences (200000168 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11205, 716397, 560, 714) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 135, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (148.80, 88.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 601) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 237, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685) [M::mem_pestat] mean and std.dev: (264.51, 125.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 857) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1570, 3323, 6140) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15280) [M::mem_pestat] mean and std.dev: (3959.85, 2790.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19850) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1147, 3151, 5481) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14149) [M::mem_pestat] mean and std.dev: (3603.18, 2808.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18483) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1982120 reads in 203.546 CPU sec, 12.949 real sec [M::process] read 1982368 sequences (200000145 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11569, 715535, 568, 640) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 133, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 468) [M::mem_pestat] mean and std.dev: (147.54, 89.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 596) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 237, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685) [M::mem_pestat] mean and std.dev: (264.49, 125.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 857) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1733, 3540, 6210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15164) [M::mem_pestat] mean and std.dev: (4038.14, 2793.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19641) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1047, 2982, 5502) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14412) [M::mem_pestat] mean and std.dev: (3505.23, 2818.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18867) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1982240 reads in 204.893 CPU sec, 13.116 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11364, 716138, 584, 684) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 134, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 463) [M::mem_pestat] mean and std.dev: (147.30, 87.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 589) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 237, 342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688) [M::mem_pestat] mean and std.dev: (264.98, 126.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 861) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1780, 3747, 6522) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16006) [M::mem_pestat] mean and std.dev: (4218.63, 2712.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20748) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1005, 2974, 5465) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14385) [M::mem_pestat] mean and std.dev: (3468.41, 2808.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18845) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1982322 sequences (200000025 bp)... [M::mem_process_seqs] Processed 1982368 reads in 206.010 CPU sec, 13.072 real sec [M::process] read 1982372 sequences (200000133 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10314, 715986, 637, 713) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 137, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (149.55, 89.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 609) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 238, 343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 689) [M::mem_pestat] mean and std.dev: (266.14, 126.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 862) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1940, 3569, 6232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14816) [M::mem_pestat] mean and std.dev: (4101.94, 2659.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19108) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1157, 3439, 5752) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14942) [M::mem_pestat] mean and std.dev: (3667.68, 2766.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19537) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1982322 reads in 210.654 CPU sec, 13.456 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9391, 717723, 538, 647) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 137, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 479) [M::mem_pestat] mean and std.dev: (149.83, 89.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 610) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 238, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 683) [M::mem_pestat] mean and std.dev: (265.27, 124.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 854) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1695, 3302, 5933) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14409) [M::mem_pestat] mean and std.dev: (3984.29, 2763.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18647) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::process] read 1982330 sequences (200000029 bp)... [M::mem_pestat] (25, 50, 75) percentile: (1151, 3039, 5367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13799) [M::mem_pestat] mean and std.dev: (3500.26, 2676.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18015) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1982372 reads in 211.258 CPU sec, 13.400 real sec [M::process] read 1982300 sequences (200000183 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10180, 717754, 561, 685) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 137, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 482) [M::mem_pestat] mean and std.dev: (150.40, 88.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 614) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 238, 340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678) [M::mem_pestat] mean and std.dev: (264.80, 123.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1800, 3598, 5913) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14139) [M::mem_pestat] mean and std.dev: (4042.28, 2646.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18252) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1098, 2778, 5313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13743) [M::mem_pestat] mean and std.dev: (3420.94, 2729.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17958) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1982330 reads in 206.845 CPU sec, 13.165 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10631, 716621, 583, 680) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 133, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469) [M::mem_pestat] mean and std.dev: (149.17, 88.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 597) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 237, 339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 677) [M::mem_pestat] mean and std.dev: (264.12, 123.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 846) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1727, 3796, 6591) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16319) [M::mem_pestat] mean and std.dev: (4233.52, 2804.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21183) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1071, 3121, 5664) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14850) [M::mem_pestat] mean and std.dev: (3555.47, 2835.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19443) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1982354 sequences (200000054 bp)... [M::mem_process_seqs] Processed 1982300 reads in 206.481 CPU sec, 13.128 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10806, 717618, 580, 667) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 135, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 463) [M::mem_pestat] mean and std.dev: (147.24, 86.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 589) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 237, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674) [M::mem_pestat] mean and std.dev: (263.28, 122.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 842) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1656, 3519, 6012) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14724) [M::mem_pestat] mean and std.dev: (4012.34, 2745.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19080) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1335, 3197, 5665) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14325) [M::mem_pestat] mean and std.dev: (3693.42, 2754.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18655) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1982522 sequences (200000134 bp)... [M::mem_process_seqs] Processed 1982354 reads in 205.853 CPU sec, 13.086 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10651, 716214, 626, 664) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 136, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473) [M::mem_pestat] mean and std.dev: (148.88, 86.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 235, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664) [M::mem_pestat] mean and std.dev: (260.75, 120.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 829) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1592, 3758, 6330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15806) [M::mem_pestat] mean and std.dev: (4112.67, 2760.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20544) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1324, 3377, 5761) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14635) [M::mem_pestat] mean and std.dev: (3764.52, 2820.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19072) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1982426 sequences (200000068 bp)... [M::mem_process_seqs] Processed 1982522 reads in 206.584 CPU sec, 13.139 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9339, 717889, 595, 676) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 135, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 483) [M::mem_pestat] mean and std.dev: (148.71, 89.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 616) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 237, 339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 677) [M::mem_pestat] mean and std.dev: (263.65, 123.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 846) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1788, 3568, 5908) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14148) [M::mem_pestat] mean and std.dev: (3984.68, 2609.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18268) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1344, 3410, 6316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16260) [M::mem_pestat] mean and std.dev: (3906.50, 2929.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21232) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1982426 reads in 204.571 CPU sec, 13.009 real sec [M::process] read 1982260 sequences (200000188 bp)... [M::process] read 1982314 sequences (200000088 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9319, 717921, 581, 637) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 137, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490) [M::mem_pestat] mean and std.dev: (149.61, 89.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 625) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 238, 342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 686) [M::mem_pestat] mean and std.dev: (265.33, 125.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 858) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1679, 3506, 5986) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14600) [M::mem_pestat] mean and std.dev: (3981.08, 2680.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18907) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1216, 3182, 5927) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15349) [M::mem_pestat] mean and std.dev: (3727.15, 2922.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20060) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1982260 reads in 203.145 CPU sec, 13.027 real sec [M::process] read 1982414 sequences (200000112 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9724, 717951, 585, 707) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 136, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 491) [M::mem_pestat] mean and std.dev: (151.35, 90.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 626) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 238, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 683) [M::mem_pestat] mean and std.dev: (264.79, 125.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 854) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1668, 3540, 5794) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14046) [M::mem_pestat] mean and std.dev: (3998.67, 2688.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18172) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1164, 3089, 6049) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15819) [M::mem_pestat] mean and std.dev: (3693.92, 2889.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20704) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1982314 reads in 204.237 CPU sec, 13.016 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10007, 717869, 565, 664) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 135, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 479) [M::mem_pestat] mean and std.dev: (150.23, 90.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 610) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 238, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 683) [M::mem_pestat] mean and std.dev: (264.78, 125.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 854) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1788, 3518, 6167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14925) [M::mem_pestat] mean and std.dev: (4100.54, 2766.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19304) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1108, 3143, 5487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14245) [M::mem_pestat] mean and std.dev: (3530.99, 2786.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18624) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1982212 sequences (200000141 bp)... [M::mem_process_seqs] Processed 1982414 reads in 204.840 CPU sec, 13.013 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10497, 716528, 612, 736) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 134, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 485) [M::mem_pestat] mean and std.dev: (148.83, 91.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 619) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 238, 342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 686) [M::mem_pestat] mean and std.dev: (265.66, 126.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 858) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1773, 3478, 6101) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14757) [M::mem_pestat] mean and std.dev: (4006.94, 2699.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19085) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1513, 3420, 5933) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14773) [M::mem_pestat] mean and std.dev: (3798.32, 2762.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19193) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1982170 sequences (200000149 bp)... [M::mem_process_seqs] Processed 1982212 reads in 205.404 CPU sec, 13.081 real sec [M::process] read 1982180 sequences (200000141 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10512, 717296, 601, 644) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 135, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (148.62, 89.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 600) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 238, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 683) [M::mem_pestat] mean and std.dev: (264.91, 125.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 854) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1679, 3545, 6255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15407) [M::mem_pestat] mean and std.dev: (4081.06, 2769.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19983) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1354, 3317, 5850) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14842) [M::mem_pestat] mean and std.dev: (3739.76, 2805.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19338) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1982170 reads in 205.577 CPU sec, 13.131 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11003, 716046, 615, 694) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 133, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (148.57, 90.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 604) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 237, 342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 686) [M::mem_pestat] mean and std.dev: (265.23, 126.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 858) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1730, 3743, 6309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15467) [M::mem_pestat] mean and std.dev: (4135.10, 2746.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20046) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (960, 2884, 5569) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14787) [M::mem_pestat] mean and std.dev: (3554.61, 2886.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19396) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1982316 sequences (200000012 bp)... [M::mem_process_seqs] Processed 1982180 reads in 205.786 CPU sec, 13.050 real sec [M::process] read 1982388 sequences (200000147 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10941, 716863, 623, 640) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 135, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (149.19, 89.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 601) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 238, 342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 686) [M::mem_pestat] mean and std.dev: (265.26, 126.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 858) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1845, 3613, 6185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14865) [M::mem_pestat] mean and std.dev: (4144.56, 2760.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19205) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (997, 2829, 5495) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14491) [M::mem_pestat] mean and std.dev: (3397.68, 2727.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18989) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1982316 reads in 207.071 CPU sec, 13.207 real sec [M::process] read 18798 sequences (1896552 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11004, 716399, 575, 661) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 133, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 485) [M::mem_pestat] mean and std.dev: (148.62, 90.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 619) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 238, 342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 686) [M::mem_pestat] mean and std.dev: (265.33, 125.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 858) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1737, 3679, 6344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15558) [M::mem_pestat] mean and std.dev: (4171.17, 2756.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20165) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1244, 3092, 5758) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14786) [M::mem_pestat] mean and std.dev: (3656.83, 2819.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19300) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1982388 reads in 206.357 CPU sec, 13.083 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (111, 6845, 4, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 130, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (150.04, 108.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 656) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 240, 345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695) [M::mem_pestat] mean and std.dev: (266.75, 126.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 870) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 18798 reads in 1.972 CPU sec, 0.131 real sec [main] Version: 0.7.17-r1188 [main] CMD: bwa mem -SP5M -t 20 /oak/stanford/groups/akundaje/marinovg/genomes/Phaeophyta/Scytosiphon_promiscuus_MALE/bwa-indexes/Scytosiphon_promiscuus_MALE.fa /oak/stanford/groups/akundaje/marinovg/papers/2024_ToL_Hi-C/2023-07-09/13-brown-algae/juicer-Scytosiphon_promiscuus_gametophyte_male-SRX23262819/splits/Scytosiphon_promiscuus_gametophyte_male-SRX23262819_R1.fastq.gz /oak/stanford/groups/akundaje/marinovg/papers/2024_ToL_Hi-C/2023-07-09/13-brown-algae/juicer-Scytosiphon_promiscuus_gametophyte_male-SRX23262819/splits/Scytosiphon_promiscuus_gametophyte_male-SRX23262819_R2.fastq.gz [main] Real time: 781.898 sec; CPU: 11904.450 sec Picked up _JAVA_OPTIONS: -Xmx16384m Picked up _JAVA_OPTIONS: -Xmx16384m Picked up _JAVA_OPTIONS: -Xmx16384m Picked up _JAVA_OPTIONS: -Xmx16384m