[M::bwa_idx_load_from_disk] read 0 ALT contigs [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3336, 93171, 3309, 3352) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (677, 1710, 4171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11159) [M::mem_pestat] mean and std.dev: (2724.45, 2574.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14653) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 377, 483) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 845) [M::mem_pestat] mean and std.dev: (392.88, 134.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1026) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (663, 1761, 4220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11334) [M::mem_pestat] mean and std.dev: (2738.09, 2604.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14891) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (653, 1658, 4171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11207) [M::mem_pestat] mean and std.dev: (2678.66, 2550.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14725) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 370.599 CPU sec, 52.125 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3417, 93559, 3316, 3311) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (664, 1611, 4054) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10834) [M::mem_pestat] mean and std.dev: (2674.65, 2572.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14224) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (300, 376, 483) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 849) [M::mem_pestat] mean and std.dev: (391.89, 134.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1032) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (632, 1697, 4201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11339) [M::mem_pestat] mean and std.dev: (2741.57, 2635.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14908) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (687, 1674, 4030) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10716) [M::mem_pestat] mean and std.dev: (2668.19, 2547.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14059) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 262.324 CPU sec, 16.844 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3541, 92628, 3378, 3394) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (650, 1620, 3894) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10382) [M::mem_pestat] mean and std.dev: (2640.42, 2553.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13626) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (301, 378, 484) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 850) [M::mem_pestat] mean and std.dev: (393.09, 135.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1033) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (623, 1654, 4248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11498) [M::mem_pestat] mean and std.dev: (2749.34, 2694.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15123) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (677, 1753, 4222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11312) [M::mem_pestat] mean and std.dev: (2739.24, 2584.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14857) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 260.668 CPU sec, 16.618 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3399, 93147, 3235, 3458) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (663, 1761, 4321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11637) [M::mem_pestat] mean and std.dev: (2761.96, 2623.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15295) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (301, 378, 485) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 853) [M::mem_pestat] mean and std.dev: (394.05, 136.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1037) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (628, 1641, 4167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11245) [M::mem_pestat] mean and std.dev: (2742.70, 2663.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14784) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (623, 1588, 4065) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10949) [M::mem_pestat] mean and std.dev: (2649.85, 2573.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14391) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 259.108 CPU sec, 16.670 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3448, 92769, 3221, 3501) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (623, 1615, 4030) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10844) [M::mem_pestat] mean and std.dev: (2672.82, 2603.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14251) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (301, 378, 485) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 853) [M::mem_pestat] mean and std.dev: (393.34, 135.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1037) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (626, 1769, 4454) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12110) [M::mem_pestat] mean and std.dev: (2805.54, 2661.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15938) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (650, 1641, 4260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11480) [M::mem_pestat] mean and std.dev: (2772.71, 2675.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15090) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 260.411 CPU sec, 16.688 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3351, 92505, 3427, 3471) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (679, 1749, 4059) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10819) [M::mem_pestat] mean and std.dev: (2753.88, 2640.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14199) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 378, 485) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 851) [M::mem_pestat] mean and std.dev: (395.05, 135.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1034) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (649, 1749, 4342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11728) [M::mem_pestat] mean and std.dev: (2799.87, 2700.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15421) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (652, 1635, 4127) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11077) [M::mem_pestat] mean and std.dev: (2721.72, 2608.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14552) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 262.974 CPU sec, 16.908 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3497, 93028, 3232, 3542) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (643, 1600, 4219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11371) [M::mem_pestat] mean and std.dev: (2686.44, 2599.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14947) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (301, 378, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 862) [M::mem_pestat] mean and std.dev: (394.87, 136.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1049) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (648, 1641, 3976) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10632) [M::mem_pestat] mean and std.dev: (2647.66, 2565.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13960) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (635, 1578, 4193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11309) [M::mem_pestat] mean and std.dev: (2707.31, 2623.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14867) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 260.763 CPU sec, 16.602 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3453, 93337, 3373, 3465) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (670, 1690, 4357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11731) [M::mem_pestat] mean and std.dev: (2772.51, 2629.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15418) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (301, 378, 486) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 856) [M::mem_pestat] mean and std.dev: (394.23, 136.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1041) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (556, 1539, 4120) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11248) [M::mem_pestat] mean and std.dev: (2658.64, 2648.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14812) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (682, 1771, 4343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11665) [M::mem_pestat] mean and std.dev: (2802.50, 2664.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15326) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 261.919 CPU sec, 16.753 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3343, 92946, 3196, 3447) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (653, 1604, 3965) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10589) [M::mem_pestat] mean and std.dev: (2670.20, 2623.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13901) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 380, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860) [M::mem_pestat] mean and std.dev: (396.08, 137.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1046) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (610, 1688, 4147) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11221) [M::mem_pestat] mean and std.dev: (2670.00, 2572.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14758) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (667, 1709, 4477) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12097) [M::mem_pestat] mean and std.dev: (2813.06, 2692.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15907) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 260.925 CPU sec, 16.832 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3366, 92198, 3414, 3499) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (637, 1591, 4128) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11110) [M::mem_pestat] mean and std.dev: (2629.11, 2525.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14601) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 379, 487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 857) [M::mem_pestat] mean and std.dev: (395.36, 136.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1042) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (595, 1593, 4097) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11101) [M::mem_pestat] mean and std.dev: (2695.30, 2651.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14603) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (619, 1578, 4082) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11008) [M::mem_pestat] mean and std.dev: (2648.87, 2570.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14471) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 258.890 CPU sec, 16.437 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3592, 92978, 3308, 3417) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (670, 1697, 4202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11266) [M::mem_pestat] mean and std.dev: (2717.30, 2594.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14798) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 379, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 858) [M::mem_pestat] mean and std.dev: (396.23, 137.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1043) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (625, 1728, 4299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11647) [M::mem_pestat] mean and std.dev: (2790.54, 2676.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15321) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (655, 1599, 3950) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10540) [M::mem_pestat] mean and std.dev: (2652.37, 2585.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13835) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 256.864 CPU sec, 16.375 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3503, 92528, 3316, 3379) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (634, 1676, 4356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11800) [M::mem_pestat] mean and std.dev: (2769.45, 2669.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15522) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 379, 486) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 854) [M::mem_pestat] mean and std.dev: (395.01, 135.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1038) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (649, 1672, 4134) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11104) [M::mem_pestat] mean and std.dev: (2684.97, 2585.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14589) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (610, 1593, 4149) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11227) [M::mem_pestat] mean and std.dev: (2702.11, 2633.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14766) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 260.749 CPU sec, 16.630 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3424, 92436, 3372, 3486) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (681, 1688, 4279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11475) [M::mem_pestat] mean and std.dev: (2740.91, 2601.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15073) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 381, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861) [M::mem_pestat] mean and std.dev: (397.06, 138.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1047) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (622, 1642, 4209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11383) [M::mem_pestat] mean and std.dev: (2724.11, 2651.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14970) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (664, 1622, 4190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11242) [M::mem_pestat] mean and std.dev: (2705.92, 2590.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14768) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 258.921 CPU sec, 16.469 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3613, 92042, 3380, 3506) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (661, 1669, 4306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11596) [M::mem_pestat] mean and std.dev: (2729.32, 2641.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15241) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 380, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 863) [M::mem_pestat] mean and std.dev: (396.67, 137.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1050) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (600, 1660, 4371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11913) [M::mem_pestat] mean and std.dev: (2746.28, 2650.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15684) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (655, 1663, 4211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11323) [M::mem_pestat] mean and std.dev: (2739.79, 2632.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14879) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 259.215 CPU sec, 16.579 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3469, 92805, 3326, 3324) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (675, 1721, 4093) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10929) [M::mem_pestat] mean and std.dev: (2726.10, 2577.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14347) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 380, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 858) [M::mem_pestat] mean and std.dev: (396.37, 136.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1043) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (618, 1640, 4094) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11046) [M::mem_pestat] mean and std.dev: (2663.20, 2579.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14522) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (678, 1731, 4315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11589) [M::mem_pestat] mean and std.dev: (2764.03, 2601.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15226) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 259.653 CPU sec, 16.514 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3443, 92229, 3222, 3295) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (642, 1673, 4099) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11013) [M::mem_pestat] mean and std.dev: (2707.20, 2585.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14470) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 380, 487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 857) [M::mem_pestat] mean and std.dev: (396.21, 137.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1042) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (647, 1757, 4146) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11144) [M::mem_pestat] mean and std.dev: (2759.42, 2673.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14643) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (636, 1618, 4028) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10812) [M::mem_pestat] mean and std.dev: (2649.14, 2567.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14204) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 261.085 CPU sec, 16.635 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3446, 92246, 3239, 3584) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (634, 1588, 4076) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10960) [M::mem_pestat] mean and std.dev: (2641.00, 2571.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14402) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 381, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864) [M::mem_pestat] mean and std.dev: (397.59, 138.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1051) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (644, 1787, 4219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11369) [M::mem_pestat] mean and std.dev: (2750.68, 2633.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14944) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (641, 1697, 4108) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11042) [M::mem_pestat] mean and std.dev: (2690.15, 2565.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14509) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 260.777 CPU sec, 16.659 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3442, 92641, 3256, 3564) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (643, 1652, 4259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11491) [M::mem_pestat] mean and std.dev: (2721.84, 2621.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15107) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 379, 487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 857) [M::mem_pestat] mean and std.dev: (395.87, 137.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1042) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (598, 1625, 4155) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11269) [M::mem_pestat] mean and std.dev: (2691.12, 2618.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14826) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (649, 1627, 4115) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11047) [M::mem_pestat] mean and std.dev: (2697.98, 2617.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14513) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 260.991 CPU sec, 16.739 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3662, 91843, 3361, 3674) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (653, 1654, 4128) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11078) [M::mem_pestat] mean and std.dev: (2731.90, 2620.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14553) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 380, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861) [M::mem_pestat] mean and std.dev: (396.56, 136.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1047) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (608, 1732, 4215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11429) [M::mem_pestat] mean and std.dev: (2721.44, 2638.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15036) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (659, 1656, 4156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11150) [M::mem_pestat] mean and std.dev: (2707.06, 2596.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14647) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 259.864 CPU sec, 16.463 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3516, 92833, 3262, 3504) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (620, 1568, 4158) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11234) [M::mem_pestat] mean and std.dev: (2690.42, 2644.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14772) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 380, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 863) [M::mem_pestat] mean and std.dev: (396.54, 137.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1050) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (604, 1616, 4159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11269) [M::mem_pestat] mean and std.dev: (2664.85, 2604.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14824) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (635, 1623, 3929) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10517) [M::mem_pestat] mean and std.dev: (2651.72, 2608.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13811) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 261.099 CPU sec, 16.704 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3415, 92382, 3250, 3520) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (681, 1700, 4051) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10791) [M::mem_pestat] mean and std.dev: (2715.65, 2598.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14161) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 382, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864) [M::mem_pestat] mean and std.dev: (398.03, 138.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1051) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (616, 1763, 4247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11509) [M::mem_pestat] mean and std.dev: (2773.48, 2676.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15140) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (644, 1639, 4150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11162) [M::mem_pestat] mean and std.dev: (2637.37, 2541.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14668) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 259.387 CPU sec, 16.467 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3491, 92577, 3258, 3351) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (690, 1693, 4238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11334) [M::mem_pestat] mean and std.dev: (2787.58, 2687.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14882) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (304, 380, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 862) [M::mem_pestat] mean and std.dev: (397.56, 137.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1048) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (614, 1664, 4059) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10949) [M::mem_pestat] mean and std.dev: (2669.80, 2605.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14394) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (635, 1605, 4095) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11015) [M::mem_pestat] mean and std.dev: (2644.25, 2569.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14475) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 259.923 CPU sec, 16.732 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3368, 92528, 3382, 3502) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (683, 1692, 4320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11594) [M::mem_pestat] mean and std.dev: (2741.20, 2585.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15231) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 380, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860) [M::mem_pestat] mean and std.dev: (396.03, 137.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1046) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (612, 1659, 4236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11484) [M::mem_pestat] mean and std.dev: (2674.80, 2578.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15108) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (642, 1620, 4060) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10896) [M::mem_pestat] mean and std.dev: (2663.99, 2562.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14314) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 261.064 CPU sec, 16.650 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3576, 91762, 3349, 3342) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (673, 1729, 4286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11512) [M::mem_pestat] mean and std.dev: (2770.06, 2639.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15125) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 381, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 867) [M::mem_pestat] mean and std.dev: (397.62, 138.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1055) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (633, 1655, 4084) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10986) [M::mem_pestat] mean and std.dev: (2670.90, 2610.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14437) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (663, 1655, 4036) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10782) [M::mem_pestat] mean and std.dev: (2696.32, 2614.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14155) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 260.993 CPU sec, 16.616 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3500, 92443, 3337, 3381) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (690, 1791, 4246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11358) [M::mem_pestat] mean and std.dev: (2770.75, 2598.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14914) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 381, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860) [M::mem_pestat] mean and std.dev: (397.30, 137.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1046) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (665, 1767, 4341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11693) [M::mem_pestat] mean and std.dev: (2798.58, 2661.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15369) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (623, 1617, 4092) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11030) [M::mem_pestat] mean and std.dev: (2671.65, 2579.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14499) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 261.350 CPU sec, 16.652 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3458, 92480, 3227, 3383) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (642, 1640, 4322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11682) [M::mem_pestat] mean and std.dev: (2718.92, 2605.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15362) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 380, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861) [M::mem_pestat] mean and std.dev: (396.34, 136.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1047) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (594, 1571, 4277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11643) [M::mem_pestat] mean and std.dev: (2693.66, 2642.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15326) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (662, 1741, 4443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12005) [M::mem_pestat] mean and std.dev: (2796.29, 2673.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15786) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 259.651 CPU sec, 16.572 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3364, 92113, 3247, 3336) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (652, 1662, 3927) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10477) [M::mem_pestat] mean and std.dev: (2635.71, 2512.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13752) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 380, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861) [M::mem_pestat] mean and std.dev: (397.46, 137.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1047) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (648, 1699, 4213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11343) [M::mem_pestat] mean and std.dev: (2754.49, 2647.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14908) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (652, 1622, 4066) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10894) [M::mem_pestat] mean and std.dev: (2701.77, 2624.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14308) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 258.563 CPU sec, 16.496 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3431, 92764, 3338, 3413) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (633, 1667, 4181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11277) [M::mem_pestat] mean and std.dev: (2743.79, 2663.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14825) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 381, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 858) [M::mem_pestat] mean and std.dev: (396.81, 136.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1043) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (632, 1667, 4299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11633) [M::mem_pestat] mean and std.dev: (2718.71, 2603.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15300) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (652, 1756, 4336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11704) [M::mem_pestat] mean and std.dev: (2837.79, 2691.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15388) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 259.426 CPU sec, 16.609 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3334, 92457, 3215, 3459) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (638, 1641, 4039) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10841) [M::mem_pestat] mean and std.dev: (2681.32, 2603.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14242) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 379, 486) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 854) [M::mem_pestat] mean and std.dev: (395.43, 136.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1038) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (611, 1630, 4248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11522) [M::mem_pestat] mean and std.dev: (2706.21, 2652.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15159) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (649, 1679, 4199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11299) [M::mem_pestat] mean and std.dev: (2734.45, 2639.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14849) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 261.817 CPU sec, 16.714 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3447, 91829, 3193, 3373) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (659, 1637, 3997) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10673) [M::mem_pestat] mean and std.dev: (2668.53, 2561.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14011) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 379, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860) [M::mem_pestat] mean and std.dev: (395.63, 137.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1046) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (643, 1769, 4150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11164) [M::mem_pestat] mean and std.dev: (2719.74, 2596.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14671) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (674, 1680, 4084) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10904) [M::mem_pestat] mean and std.dev: (2711.34, 2571.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14314) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 259.153 CPU sec, 16.451 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3451, 92178, 3273, 3560) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (676, 1706, 4075) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10873) [M::mem_pestat] mean and std.dev: (2725.43, 2600.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14272) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 379, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860) [M::mem_pestat] mean and std.dev: (395.64, 137.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1046) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (591, 1738, 4315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11763) [M::mem_pestat] mean and std.dev: (2770.66, 2699.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15487) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (646, 1622, 3947) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10549) [M::mem_pestat] mean and std.dev: (2676.18, 2578.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13850) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 258.536 CPU sec, 16.488 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3359, 92542, 3327, 3329) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (688, 1715, 4185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11179) [M::mem_pestat] mean and std.dev: (2757.28, 2615.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14676) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 380, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864) [M::mem_pestat] mean and std.dev: (396.70, 137.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1051) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (626, 1651, 4067) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10949) [M::mem_pestat] mean and std.dev: (2669.30, 2613.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14390) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (649, 1605, 3968) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10606) [M::mem_pestat] mean and std.dev: (2663.94, 2577.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13925) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 259.663 CPU sec, 16.489 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3431, 92856, 3377, 3524) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (644, 1677, 4001) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10715) [M::mem_pestat] mean and std.dev: (2654.47, 2541.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14072) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 378, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860) [M::mem_pestat] mean and std.dev: (395.39, 137.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1046) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (595, 1639, 4190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11380) [M::mem_pestat] mean and std.dev: (2708.26, 2667.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14975) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (679, 1833, 4534) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12244) [M::mem_pestat] mean and std.dev: (2862.55, 2663.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16099) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 258.569 CPU sec, 16.475 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3425, 92369, 3420, 3395) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (658, 1615, 3883) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10333) [M::mem_pestat] mean and std.dev: (2640.59, 2546.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13558) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] (25, 50, 75) percentile: (302, 379, 487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 857) [M::mem_pestat] mean and std.dev: (395.67, 136.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1042) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (628, 1682, 4318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11698) [M::mem_pestat] mean and std.dev: (2767.70, 2683.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15388) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (628, 1694, 4088) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11008) [M::mem_pestat] mean and std.dev: (2670.52, 2562.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14468) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 260.355 CPU sec, 16.553 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3523, 92093, 3281, 3368) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (624, 1665, 4065) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10947) [M::mem_pestat] mean and std.dev: (2685.45, 2625.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14388) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 380, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861) [M::mem_pestat] mean and std.dev: (396.47, 137.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1047) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (633, 1745, 4366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11832) [M::mem_pestat] mean and std.dev: (2776.89, 2672.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15565) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (645, 1651, 4259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11487) [M::mem_pestat] mean and std.dev: (2762.01, 2667.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15101) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 260.417 CPU sec, 16.547 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3369, 92460, 3363, 3432) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (669, 1760, 3960) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10542) [M::mem_pestat] mean and std.dev: (2714.29, 2616.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13833) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 379, 487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 857) [M::mem_pestat] mean and std.dev: (395.83, 136.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1042) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (623, 1744, 4278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11588) [M::mem_pestat] mean and std.dev: (2759.01, 2646.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15243) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (707, 1699, 4072) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10802) [M::mem_pestat] mean and std.dev: (2732.96, 2589.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14167) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 260.136 CPU sec, 16.597 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3347, 92889, 3349, 3487) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (615, 1688, 3942) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10596) [M::mem_pestat] mean and std.dev: (2659.78, 2577.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13923) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 379, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860) [M::mem_pestat] mean and std.dev: (396.07, 137.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1046) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (658, 1715, 4204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11296) [M::mem_pestat] mean and std.dev: (2724.57, 2573.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14842) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (628, 1572, 3871) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10357) [M::mem_pestat] mean and std.dev: (2599.97, 2528.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13600) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 257.669 CPU sec, 16.383 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3440, 92712, 3331, 3370) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (639, 1539, 3994) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10704) [M::mem_pestat] mean and std.dev: (2676.66, 2644.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14059) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 379, 486) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 854) [M::mem_pestat] mean and std.dev: (395.51, 136.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1038) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (600, 1635, 4334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11802) [M::mem_pestat] mean and std.dev: (2712.97, 2663.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15536) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (614, 1645, 4146) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11210) [M::mem_pestat] mean and std.dev: (2651.84, 2557.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14742) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 260.432 CPU sec, 16.625 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3492, 92739, 3312, 3527) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (626, 1669, 4074) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10970) [M::mem_pestat] mean and std.dev: (2702.32, 2627.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14418) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 380, 486) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 854) [M::mem_pestat] mean and std.dev: (395.54, 136.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1038) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (631, 1724, 4165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11233) [M::mem_pestat] mean and std.dev: (2714.45, 2628.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14767) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (627, 1661, 4084) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10998) [M::mem_pestat] mean and std.dev: (2716.04, 2625.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14455) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 257.904 CPU sec, 16.451 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3386, 92145, 3229, 3468) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (645, 1640, 3987) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10671) [M::mem_pestat] mean and std.dev: (2674.24, 2594.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14013) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 378, 485) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 851) [M::mem_pestat] mean and std.dev: (394.41, 135.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1034) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (637, 1769, 4464) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12118) [M::mem_pestat] mean and std.dev: (2796.07, 2647.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15945) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (656, 1652, 4217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11339) [M::mem_pestat] mean and std.dev: (2743.92, 2647.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14900) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 261.410 CPU sec, 16.611 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3331, 92228, 3323, 3432) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (663, 1637, 4236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11382) [M::mem_pestat] mean and std.dev: (2759.33, 2658.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14955) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (301, 378, 487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 859) [M::mem_pestat] mean and std.dev: (394.73, 136.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1045) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (582, 1597, 4177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11367) [M::mem_pestat] mean and std.dev: (2683.97, 2656.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14962) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (641, 1619, 4168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11222) [M::mem_pestat] mean and std.dev: (2689.79, 2584.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14749) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 258.469 CPU sec, 16.575 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3414, 93408, 3198, 3314) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (634, 1628, 3881) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10375) [M::mem_pestat] mean and std.dev: (2616.36, 2548.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13622) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 380, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860) [M::mem_pestat] mean and std.dev: (395.65, 137.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1046) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (619, 1682, 4152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11218) [M::mem_pestat] mean and std.dev: (2710.05, 2630.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14751) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (657, 1644, 4150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11136) [M::mem_pestat] mean and std.dev: (2697.38, 2581.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14629) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 258.809 CPU sec, 16.418 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3361, 92571, 3298, 3394) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (662, 1708, 4129) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11063) [M::mem_pestat] mean and std.dev: (2687.97, 2550.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14530) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 379, 485) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 851) [M::mem_pestat] mean and std.dev: (394.32, 135.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1034) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (660, 1778, 4358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11754) [M::mem_pestat] mean and std.dev: (2802.62, 2654.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15452) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (660, 1682, 4083) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10929) [M::mem_pestat] mean and std.dev: (2707.88, 2610.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14352) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 260.191 CPU sec, 16.597 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3478, 92713, 3160, 3331) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (634, 1657, 4151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11185) [M::mem_pestat] mean and std.dev: (2680.41, 2577.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14702) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 379, 487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 857) [M::mem_pestat] mean and std.dev: (395.82, 136.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1042) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (643, 1582, 3985) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10669) [M::mem_pestat] mean and std.dev: (2626.01, 2540.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14011) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (640, 1588, 4056) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10888) [M::mem_pestat] mean and std.dev: (2671.83, 2601.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14304) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 260.739 CPU sec, 16.662 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3507, 92448, 3192, 3557) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (671, 1617, 4194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11240) [M::mem_pestat] mean and std.dev: (2711.45, 2619.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14763) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (301, 379, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 862) [M::mem_pestat] mean and std.dev: (395.50, 137.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1049) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (637, 1723, 4164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11218) [M::mem_pestat] mean and std.dev: (2737.02, 2638.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14745) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (638, 1594, 3985) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10679) [M::mem_pestat] mean and std.dev: (2650.17, 2556.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14026) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 259.265 CPU sec, 16.520 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3473, 93027, 3277, 3456) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (654, 1662, 4358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11766) [M::mem_pestat] mean and std.dev: (2782.06, 2682.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15470) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 379, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 863) [M::mem_pestat] mean and std.dev: (396.46, 137.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1050) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (638, 1649, 4051) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10877) [M::mem_pestat] mean and std.dev: (2689.34, 2585.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14290) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (657, 1718, 4118) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11040) [M::mem_pestat] mean and std.dev: (2738.41, 2631.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14501) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 260.512 CPU sec, 16.592 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3459, 92790, 3317, 3440) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (644, 1677, 4131) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11105) [M::mem_pestat] mean and std.dev: (2686.03, 2569.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14592) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (301, 379, 486) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 856) [M::mem_pestat] mean and std.dev: (395.08, 136.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1041) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (593, 1641, 4450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12164) [M::mem_pestat] mean and std.dev: (2793.95, 2754.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16021) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (634, 1607, 4057) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10903) [M::mem_pestat] mean and std.dev: (2638.71, 2550.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14326) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 261.950 CPU sec, 16.686 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3416, 91881, 3287, 3538) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (641, 1601, 4349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11765) [M::mem_pestat] mean and std.dev: (2743.30, 2659.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15473) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 379, 487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 857) [M::mem_pestat] mean and std.dev: (395.33, 136.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1042) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (609, 1611, 3968) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10686) [M::mem_pestat] mean and std.dev: (2614.94, 2563.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14045) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (693, 1734, 4098) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10908) [M::mem_pestat] mean and std.dev: (2724.53, 2562.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14313) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 261.422 CPU sec, 16.671 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3359, 92319, 3283, 3412) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (660, 1632, 4031) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10773) [M::mem_pestat] mean and std.dev: (2669.95, 2561.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14144) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (301, 378, 485) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 853) [M::mem_pestat] mean and std.dev: (394.22, 136.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1037) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (613, 1694, 4206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11392) [M::mem_pestat] mean and std.dev: (2734.69, 2647.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14985) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (655, 1631, 3778) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10024) [M::mem_pestat] mean and std.dev: (2619.10, 2550.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13147) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 258.761 CPU sec, 16.463 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3473, 92999, 3291, 3356) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (695, 1697, 4031) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10703) [M::mem_pestat] mean and std.dev: (2699.72, 2551.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14039) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (301, 378, 486) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 856) [M::mem_pestat] mean and std.dev: (395.01, 136.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1041) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (661, 1738, 4253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11437) [M::mem_pestat] mean and std.dev: (2745.01, 2618.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15029) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (665, 1629, 4035) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10775) [M::mem_pestat] mean and std.dev: (2662.84, 2563.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14145) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 260.606 CPU sec, 16.581 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3392, 92480, 3314, 3412) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (642, 1654, 4057) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10887) [M::mem_pestat] mean and std.dev: (2676.04, 2566.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14302) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (301, 378, 486) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 856) [M::mem_pestat] mean and std.dev: (394.56, 137.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1041) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (631, 1694, 4183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11287) [M::mem_pestat] mean and std.dev: (2726.59, 2639.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14839) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (635, 1613, 4116) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11078) [M::mem_pestat] mean and std.dev: (2685.09, 2612.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14559) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 259.547 CPU sec, 16.548 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3323, 92492, 3395, 3368) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (685, 1652, 4209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11257) [M::mem_pestat] mean and std.dev: (2727.72, 2627.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14781) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (301, 378, 486) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 856) [M::mem_pestat] mean and std.dev: (394.72, 136.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1041) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (618, 1642, 4200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11364) [M::mem_pestat] mean and std.dev: (2713.65, 2638.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14946) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (641, 1532, 3871) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10331) [M::mem_pestat] mean and std.dev: (2595.66, 2545.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13561) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 260.894 CPU sec, 16.602 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3408, 92642, 3342, 3489) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (620, 1605, 4058) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10934) [M::mem_pestat] mean and std.dev: (2662.58, 2589.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14372) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 379, 487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 857) [M::mem_pestat] mean and std.dev: (395.72, 136.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1042) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (644, 1691, 4170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11222) [M::mem_pestat] mean and std.dev: (2720.94, 2612.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14748) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (673, 1757, 4300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11554) [M::mem_pestat] mean and std.dev: (2765.56, 2598.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15181) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 258.397 CPU sec, 16.544 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3425, 92753, 3281, 3272) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (640, 1729, 4424) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11992) [M::mem_pestat] mean and std.dev: (2786.75, 2642.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15776) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 379, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860) [M::mem_pestat] mean and std.dev: (395.51, 137.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1046) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (622, 1686, 4333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11755) [M::mem_pestat] mean and std.dev: (2725.18, 2624.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15466) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (631, 1647, 4028) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10822) [M::mem_pestat] mean and std.dev: (2643.79, 2535.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14219) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 260.072 CPU sec, 16.962 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3422, 92955, 3138, 3392) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (668, 1772, 4183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11213) [M::mem_pestat] mean and std.dev: (2768.39, 2622.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14728) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (301, 378, 486) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 856) [M::mem_pestat] mean and std.dev: (394.81, 136.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1041) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (612, 1651, 4260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11556) [M::mem_pestat] mean and std.dev: (2704.53, 2626.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15204) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (639, 1642, 4153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11181) [M::mem_pestat] mean and std.dev: (2707.81, 2620.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14695) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 259.897 CPU sec, 16.788 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3276, 92762, 3395, 3356) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (622, 1564, 4244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11488) [M::mem_pestat] mean and std.dev: (2725.15, 2686.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15110) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 378, 487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 857) [M::mem_pestat] mean and std.dev: (395.19, 136.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1042) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (658, 1744, 4288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11548) [M::mem_pestat] mean and std.dev: (2781.95, 2677.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15178) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (642, 1581, 4027) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10797) [M::mem_pestat] mean and std.dev: (2635.19, 2551.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14182) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 262.493 CPU sec, 16.664 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3387, 92571, 3190, 3443) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (657, 1635, 3876) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10314) [M::mem_pestat] mean and std.dev: (2632.65, 2548.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13533) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (301, 379, 486) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 856) [M::mem_pestat] mean and std.dev: (394.67, 136.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1041) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (617, 1706, 4221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11429) [M::mem_pestat] mean and std.dev: (2754.46, 2663.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15033) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (605, 1599, 4095) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11075) [M::mem_pestat] mean and std.dev: (2650.53, 2603.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14565) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 264.835 CPU sec, 16.879 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3410, 92933, 3372, 3519) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (657, 1610, 4066) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10884) [M::mem_pestat] mean and std.dev: (2688.97, 2623.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14293) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (301, 378, 487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 859) [M::mem_pestat] mean and std.dev: (394.77, 137.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1045) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (622, 1693, 4290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11626) [M::mem_pestat] mean and std.dev: (2736.88, 2649.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15294) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (668, 1706, 4145) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11099) [M::mem_pestat] mean and std.dev: (2733.67, 2609.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14576) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 262.091 CPU sec, 16.969 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3483, 92749, 3159, 3382) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (684, 1771, 4293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11511) [M::mem_pestat] mean and std.dev: (2789.43, 2630.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15120) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (301, 378, 485) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 853) [M::mem_pestat] mean and std.dev: (394.24, 135.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1037) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (646, 1745, 4517) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12259) [M::mem_pestat] mean and std.dev: (2814.66, 2672.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16130) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (626, 1561, 3892) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10424) [M::mem_pestat] mean and std.dev: (2595.99, 2530.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13690) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 262.155 CPU sec, 16.658 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3519, 92966, 3284, 3384) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (634, 1578, 3854) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10294) [M::mem_pestat] mean and std.dev: (2624.30, 2594.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13514) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (301, 378, 485) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 853) [M::mem_pestat] mean and std.dev: (394.33, 135.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1037) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (651, 1634, 4320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11658) [M::mem_pestat] mean and std.dev: (2761.69, 2674.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15327) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (632, 1629, 4158) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11210) [M::mem_pestat] mean and std.dev: (2696.77, 2635.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14736) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 262.005 CPU sec, 16.740 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3452, 92998, 3363, 3574) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (597, 1561, 4080) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11046) [M::mem_pestat] mean and std.dev: (2632.81, 2593.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14529) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (300, 377, 485) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 855) [M::mem_pestat] mean and std.dev: (393.65, 136.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1040) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (596, 1598, 4174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11330) [M::mem_pestat] mean and std.dev: (2699.18, 2658.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14908) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (694, 1717, 4130) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11002) [M::mem_pestat] mean and std.dev: (2759.37, 2608.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14438) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 261.476 CPU sec, 16.687 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3458, 93905, 3312, 3454) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (612, 1625, 3852) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10332) [M::mem_pestat] mean and std.dev: (2607.66, 2531.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13572) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (301, 378, 486) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 856) [M::mem_pestat] mean and std.dev: (394.71, 136.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1041) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (593, 1696, 4193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11393) [M::mem_pestat] mean and std.dev: (2714.80, 2663.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14993) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (633, 1582, 4162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11220) [M::mem_pestat] mean and std.dev: (2682.63, 2631.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14749) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 259.624 CPU sec, 16.714 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3434, 92877, 3315, 3504) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (657, 1720, 4215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11331) [M::mem_pestat] mean and std.dev: (2746.32, 2613.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14889) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (300, 378, 484) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 852) [M::mem_pestat] mean and std.dev: (393.31, 135.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1036) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (640, 1608, 3949) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10567) [M::mem_pestat] mean and std.dev: (2661.50, 2613.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13876) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (618, 1662, 4163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11253) [M::mem_pestat] mean and std.dev: (2712.67, 2624.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14798) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 263.158 CPU sec, 16.682 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3386, 92668, 3266, 3420) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (642, 1606, 4032) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10812) [M::mem_pestat] mean and std.dev: (2667.81, 2600.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14202) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 379, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860) [M::mem_pestat] mean and std.dev: (395.46, 136.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1046) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (612, 1648, 4145) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11211) [M::mem_pestat] mean and std.dev: (2714.41, 2657.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14744) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (656, 1613, 4035) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10793) [M::mem_pestat] mean and std.dev: (2666.23, 2589.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14172) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 258.595 CPU sec, 16.512 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3460, 92711, 3385, 3412) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (668, 1678, 4050) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10814) [M::mem_pestat] mean and std.dev: (2699.09, 2591.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14196) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 379, 487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 857) [M::mem_pestat] mean and std.dev: (394.93, 136.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1042) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (658, 1755, 4455) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12049) [M::mem_pestat] mean and std.dev: (2816.27, 2673.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15846) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (638, 1617, 4051) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10877) [M::mem_pestat] mean and std.dev: (2629.81, 2529.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14290) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 259.586 CPU sec, 16.456 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3389, 92561, 3295, 3582) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (659, 1681, 4165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11177) [M::mem_pestat] mean and std.dev: (2698.53, 2590.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14683) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (301, 379, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 862) [M::mem_pestat] mean and std.dev: (395.60, 137.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1049) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (648, 1762, 4340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11724) [M::mem_pestat] mean and std.dev: (2762.08, 2620.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15416) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (657, 1596, 4035) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10791) [M::mem_pestat] mean and std.dev: (2644.98, 2558.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14169) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 261.659 CPU sec, 16.643 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3399, 92457, 3169, 3537) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (676, 1697, 4300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11548) [M::mem_pestat] mean and std.dev: (2769.14, 2607.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15172) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 379, 487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 857) [M::mem_pestat] mean and std.dev: (395.66, 137.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1042) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (619, 1673, 3987) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10723) [M::mem_pestat] mean and std.dev: (2624.80, 2549.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14091) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (666, 1732, 4241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11391) [M::mem_pestat] mean and std.dev: (2798.26, 2648.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14966) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 260.953 CPU sec, 16.639 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3367, 93002, 3410, 3462) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (639, 1784, 4168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11226) [M::mem_pestat] mean and std.dev: (2734.75, 2595.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14755) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 379, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 863) [M::mem_pestat] mean and std.dev: (396.28, 138.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1050) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (590, 1605, 3875) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10445) [M::mem_pestat] mean and std.dev: (2616.48, 2573.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13730) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (634, 1653, 4084) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10984) [M::mem_pestat] mean and std.dev: (2667.20, 2556.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14434) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 262.793 CPU sec, 16.715 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3312, 92048, 3327, 3413) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (677, 1712, 4321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11609) [M::mem_pestat] mean and std.dev: (2784.75, 2651.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15253) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 380, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 863) [M::mem_pestat] mean and std.dev: (396.79, 138.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1050) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (587, 1603, 4279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11663) [M::mem_pestat] mean and std.dev: (2670.67, 2615.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15355) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (622, 1635, 4017) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10807) [M::mem_pestat] mean and std.dev: (2678.13, 2581.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14202) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 261.017 CPU sec, 16.642 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3530, 92380, 3234, 3402) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (661, 1690, 4297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11569) [M::mem_pestat] mean and std.dev: (2784.62, 2677.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15205) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 381, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864) [M::mem_pestat] mean and std.dev: (397.52, 137.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1051) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (649, 1696, 4238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11416) [M::mem_pestat] mean and std.dev: (2740.18, 2615.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15005) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (612, 1642, 4000) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10776) [M::mem_pestat] mean and std.dev: (2657.00, 2583.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14164) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 259.572 CPU sec, 16.657 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3441, 93116, 3253, 3394) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (635, 1608, 4161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11213) [M::mem_pestat] mean and std.dev: (2721.81, 2659.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14739) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 380, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 858) [M::mem_pestat] mean and std.dev: (396.56, 137.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1043) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (635, 1693, 4226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11408) [M::mem_pestat] mean and std.dev: (2762.10, 2644.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14999) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (673, 1733, 4075) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10879) [M::mem_pestat] mean and std.dev: (2737.52, 2616.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14281) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 259.434 CPU sec, 16.533 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3386, 92789, 3217, 3532) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (657, 1631, 4059) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10863) [M::mem_pestat] mean and std.dev: (2686.92, 2600.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14265) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 380, 486) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 854) [M::mem_pestat] mean and std.dev: (395.35, 136.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1038) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (552, 1488, 4026) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10974) [M::mem_pestat] mean and std.dev: (2582.66, 2618.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14448) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (632, 1671, 4048) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10880) [M::mem_pestat] mean and std.dev: (2723.39, 2624.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14296) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 261.503 CPU sec, 16.661 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3450, 92235, 3273, 3454) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (668, 1611, 4123) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11033) [M::mem_pestat] mean and std.dev: (2714.56, 2609.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14488) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 381, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 867) [M::mem_pestat] mean and std.dev: (398.33, 138.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1055) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (616, 1708, 4248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11512) [M::mem_pestat] mean and std.dev: (2702.45, 2575.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15144) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (657, 1613, 3903) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10395) [M::mem_pestat] mean and std.dev: (2599.74, 2507.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13641) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 258.757 CPU sec, 16.520 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3364, 92529, 3333, 3533) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (689, 1753, 4192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11198) [M::mem_pestat] mean and std.dev: (2762.81, 2590.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14701) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 380, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861) [M::mem_pestat] mean and std.dev: (397.05, 137.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1047) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (611, 1680, 4247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11519) [M::mem_pestat] mean and std.dev: (2746.22, 2695.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15155) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (680, 1615, 4011) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10673) [M::mem_pestat] mean and std.dev: (2674.28, 2580.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14004) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 258.530 CPU sec, 16.432 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3509, 92342, 3320, 3442) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (674, 1834, 4485) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12107) [M::mem_pestat] mean and std.dev: (2848.22, 2675.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15918) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 381, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 867) [M::mem_pestat] mean and std.dev: (397.87, 138.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1055) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (598, 1616, 4123) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11173) [M::mem_pestat] mean and std.dev: (2651.87, 2580.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14698) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (655, 1675, 4088) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10954) [M::mem_pestat] mean and std.dev: (2723.22, 2610.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14387) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 260.080 CPU sec, 16.562 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3483, 91790, 3382, 3542) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (640, 1546, 4068) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10924) [M::mem_pestat] mean and std.dev: (2631.91, 2548.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14352) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 380, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861) [M::mem_pestat] mean and std.dev: (397.16, 137.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1047) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (662, 1772, 4301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11579) [M::mem_pestat] mean and std.dev: (2745.57, 2606.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15218) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (646, 1580, 3936) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10516) [M::mem_pestat] mean and std.dev: (2620.19, 2528.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13806) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 260.241 CPU sec, 16.567 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3511, 91568, 3267, 3442) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (668, 1689, 4226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11342) [M::mem_pestat] mean and std.dev: (2750.34, 2621.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14900) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 381, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 867) [M::mem_pestat] mean and std.dev: (398.00, 138.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1055) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (611, 1701, 4171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11291) [M::mem_pestat] mean and std.dev: (2722.37, 2619.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14851) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (632, 1533, 4081) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10979) [M::mem_pestat] mean and std.dev: (2647.91, 2601.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14428) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 260.171 CPU sec, 16.647 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3406, 91892, 3327, 3339) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (640, 1618, 3974) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10642) [M::mem_pestat] mean and std.dev: (2623.15, 2542.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13976) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 380, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861) [M::mem_pestat] mean and std.dev: (396.70, 137.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1047) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (590, 1663, 4243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11549) [M::mem_pestat] mean and std.dev: (2687.07, 2606.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15202) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (670, 1698, 4145) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11095) [M::mem_pestat] mean and std.dev: (2745.26, 2623.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14570) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 260.479 CPU sec, 16.603 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3455, 91858, 3448, 3517) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (648, 1621, 4017) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10755) [M::mem_pestat] mean and std.dev: (2649.89, 2578.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14124) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 380, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 867) [M::mem_pestat] mean and std.dev: (398.29, 139.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1055) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (607, 1703, 4089) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11053) [M::mem_pestat] mean and std.dev: (2691.74, 2610.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14535) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (682, 1753, 4338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11650) [M::mem_pestat] mean and std.dev: (2812.42, 2680.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15306) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 259.980 CPU sec, 16.476 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3524, 91964, 3259, 3427) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (677, 1703, 4211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11279) [M::mem_pestat] mean and std.dev: (2740.60, 2613.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14813) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 381, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 867) [M::mem_pestat] mean and std.dev: (398.05, 138.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1055) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (605, 1749, 4406) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12008) [M::mem_pestat] mean and std.dev: (2754.97, 2653.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15809) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (630, 1635, 4028) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10824) [M::mem_pestat] mean and std.dev: (2662.18, 2588.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14222) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 259.024 CPU sec, 16.479 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3511, 92078, 3275, 3522) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (598, 1570, 4118) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11158) [M::mem_pestat] mean and std.dev: (2637.99, 2601.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14678) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 381, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861) [M::mem_pestat] mean and std.dev: (396.96, 136.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1047) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (598, 1729, 4462) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12190) [M::mem_pestat] mean and std.dev: (2822.05, 2735.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16054) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (591, 1501, 3891) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10491) [M::mem_pestat] mean and std.dev: (2599.97, 2586.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13791) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 261.958 CPU sec, 17.092 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3351, 92104, 3314, 3435) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (631, 1608, 3923) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10507) [M::mem_pestat] mean and std.dev: (2606.40, 2521.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13799) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 380, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864) [M::mem_pestat] mean and std.dev: (397.61, 138.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1051) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (619, 1548, 3932) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10558) [M::mem_pestat] mean and std.dev: (2580.82, 2536.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13871) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (652, 1637, 3998) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10690) [M::mem_pestat] mean and std.dev: (2710.54, 2637.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14036) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 261.564 CPU sec, 17.057 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3346, 92022, 3299, 3451) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (633, 1631, 3963) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10623) [M::mem_pestat] mean and std.dev: (2669.29, 2619.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13953) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 380, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864) [M::mem_pestat] mean and std.dev: (397.63, 137.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1051) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (617, 1611, 4043) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10895) [M::mem_pestat] mean and std.dev: (2654.26, 2602.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14321) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (637, 1608, 4049) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10873) [M::mem_pestat] mean and std.dev: (2636.93, 2561.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14285) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 259.723 CPU sec, 16.537 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3387, 92222, 3241, 3427) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (613, 1623, 4200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11374) [M::mem_pestat] mean and std.dev: (2682.71, 2599.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14961) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 382, 492) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 872) [M::mem_pestat] mean and std.dev: (398.47, 139.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1062) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (627, 1585, 4170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11256) [M::mem_pestat] mean and std.dev: (2688.17, 2633.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14799) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (663, 1636, 4100) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10974) [M::mem_pestat] mean and std.dev: (2715.26, 2641.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14411) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 263.091 CPU sec, 16.949 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3450, 92427, 3303, 3223) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (671, 1656, 4080) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10898) [M::mem_pestat] mean and std.dev: (2698.90, 2598.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14307) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (304, 380, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 865) [M::mem_pestat] mean and std.dev: (398.65, 139.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1052) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (631, 1781, 4423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12007) [M::mem_pestat] mean and std.dev: (2775.60, 2640.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15799) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (641, 1788, 4297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11609) [M::mem_pestat] mean and std.dev: (2805.65, 2635.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15265) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 259.123 CPU sec, 16.436 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3493, 92251, 3361, 3389) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (620, 1555, 3774) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10082) [M::mem_pestat] mean and std.dev: (2564.90, 2536.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13236) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (304, 382, 492) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 868) [M::mem_pestat] mean and std.dev: (399.08, 138.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1056) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (616, 1704, 4247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11509) [M::mem_pestat] mean and std.dev: (2743.35, 2669.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15140) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (654, 1655, 3903) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10401) [M::mem_pestat] mean and std.dev: (2621.85, 2516.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13650) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 259.188 CPU sec, 16.514 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3530, 92149, 3119, 3407) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (637, 1676, 4264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11518) [M::mem_pestat] mean and std.dev: (2736.58, 2627.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15145) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (304, 381, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 862) [M::mem_pestat] mean and std.dev: (397.67, 137.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1048) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (616, 1694, 4111) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11101) [M::mem_pestat] mean and std.dev: (2704.93, 2622.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14596) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (650, 1639, 3919) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10457) [M::mem_pestat] mean and std.dev: (2618.47, 2538.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13726) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 258.899 CPU sec, 16.588 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3461, 91941, 3367, 3416) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (655, 1675, 4268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11494) [M::mem_pestat] mean and std.dev: (2763.85, 2662.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15107) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 381, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864) [M::mem_pestat] mean and std.dev: (397.70, 137.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1051) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (650, 1773, 4147) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11141) [M::mem_pestat] mean and std.dev: (2697.88, 2550.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14638) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (667, 1635, 4112) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11002) [M::mem_pestat] mean and std.dev: (2684.39, 2584.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14447) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 259.810 CPU sec, 16.475 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3422, 92084, 3353, 3499) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (626, 1680, 4068) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10952) [M::mem_pestat] mean and std.dev: (2674.38, 2565.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14394) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (304, 381, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 862) [M::mem_pestat] mean and std.dev: (397.80, 137.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1048) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (610, 1596, 4245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11515) [M::mem_pestat] mean and std.dev: (2714.73, 2648.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15150) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (654, 1624, 4099) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10989) [M::mem_pestat] mean and std.dev: (2694.17, 2609.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14434) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 259.943 CPU sec, 16.616 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3240, 92984, 3342, 3377) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (657, 1676, 4044) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10818) [M::mem_pestat] mean and std.dev: (2704.32, 2596.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14205) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (304, 381, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 862) [M::mem_pestat] mean and std.dev: (398.04, 138.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1048) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (634, 1618, 4142) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11158) [M::mem_pestat] mean and std.dev: (2664.50, 2600.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14666) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (649, 1662, 4223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11371) [M::mem_pestat] mean and std.dev: (2716.97, 2605.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14945) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 259.962 CPU sec, 16.475 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3391, 91833, 3206, 3515) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (650, 1683, 4349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11747) [M::mem_pestat] mean and std.dev: (2746.47, 2610.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15446) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 380, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864) [M::mem_pestat] mean and std.dev: (397.07, 137.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1051) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (575, 1645, 4165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11345) [M::mem_pestat] mean and std.dev: (2679.35, 2607.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14935) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (642, 1691, 4030) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10806) [M::mem_pestat] mean and std.dev: (2656.51, 2549.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14194) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 260.538 CPU sec, 16.576 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3482, 92362, 3258, 3354) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (670, 1598, 4326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11638) [M::mem_pestat] mean and std.dev: (2724.60, 2605.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15294) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (304, 381, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 865) [M::mem_pestat] mean and std.dev: (398.11, 137.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1052) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (608, 1678, 4131) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11177) [M::mem_pestat] mean and std.dev: (2684.14, 2643.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14700) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (619, 1683, 4117) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11113) [M::mem_pestat] mean and std.dev: (2690.58, 2611.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14611) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 261.509 CPU sec, 16.633 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3387, 91825, 3259, 3497) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (626, 1529, 4100) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11048) [M::mem_pestat] mean and std.dev: (2669.43, 2609.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14522) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 381, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 867) [M::mem_pestat] mean and std.dev: (398.21, 139.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1055) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (605, 1623, 4107) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11111) [M::mem_pestat] mean and std.dev: (2683.62, 2650.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14613) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (652, 1605, 3999) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10693) [M::mem_pestat] mean and std.dev: (2647.85, 2564.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14040) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 259.892 CPU sec, 16.614 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3418, 92617, 3314, 3459) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (662, 1635, 4287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11537) [M::mem_pestat] mean and std.dev: (2731.90, 2612.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15162) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 381, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861) [M::mem_pestat] mean and std.dev: (397.29, 137.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1047) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (641, 1677, 4106) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11036) [M::mem_pestat] mean and std.dev: (2719.77, 2625.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14501) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (637, 1549, 3920) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10486) [M::mem_pestat] mean and std.dev: (2604.24, 2542.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13769) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 261.467 CPU sec, 16.561 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3523, 91952, 3308, 3349) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (658, 1674, 4126) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11062) [M::mem_pestat] mean and std.dev: (2722.03, 2603.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14530) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 379, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861) [M::mem_pestat] mean and std.dev: (396.43, 136.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1047) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (571, 1630, 4006) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10876) [M::mem_pestat] mean and std.dev: (2618.42, 2578.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14311) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (640, 1630, 3990) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10690) [M::mem_pestat] mean and std.dev: (2679.78, 2591.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14040) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 260.877 CPU sec, 16.606 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3478, 92021, 3305, 3503) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (670, 1714, 4229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11347) [M::mem_pestat] mean and std.dev: (2722.53, 2584.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14906) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 381, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864) [M::mem_pestat] mean and std.dev: (397.40, 137.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1051) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (608, 1633, 4147) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11225) [M::mem_pestat] mean and std.dev: (2678.19, 2624.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14764) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (673, 1612, 4013) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10693) [M::mem_pestat] mean and std.dev: (2690.99, 2617.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14033) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 262.507 CPU sec, 16.759 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3424, 92226, 3233, 3379) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (644, 1540, 3852) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10268) [M::mem_pestat] mean and std.dev: (2608.58, 2543.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13476) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 379, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 858) [M::mem_pestat] mean and std.dev: (396.69, 137.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1043) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] (25, 50, 75) percentile: (594, 1541, 4085) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11067) [M::mem_pestat] mean and std.dev: (2667.91, 2648.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14558) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (647, 1676, 4126) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11084) [M::mem_pestat] mean and std.dev: (2725.39, 2636.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14563) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 259.011 CPU sec, 16.540 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3432, 92423, 3416, 3330) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (627, 1659, 4128) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11130) [M::mem_pestat] mean and std.dev: (2693.95, 2608.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14631) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 380, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861) [M::mem_pestat] mean and std.dev: (396.85, 137.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1047) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (635, 1625, 4048) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10874) [M::mem_pestat] mean and std.dev: (2674.75, 2625.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14287) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (671, 1624, 4022) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10724) [M::mem_pestat] mean and std.dev: (2693.38, 2590.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14075) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 262.064 CPU sec, 16.763 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3345, 92476, 3332, 3350) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (659, 1609, 4124) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11054) [M::mem_pestat] mean and std.dev: (2714.70, 2635.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14519) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 379, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860) [M::mem_pestat] mean and std.dev: (396.03, 137.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1046) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (624, 1750, 4142) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11178) [M::mem_pestat] mean and std.dev: (2717.51, 2623.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14696) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (697, 1727, 4311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11539) [M::mem_pestat] mean and std.dev: (2791.55, 2608.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15153) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 260.371 CPU sec, 16.508 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3337, 92633, 3232, 3490) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (679, 1669, 4332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11638) [M::mem_pestat] mean and std.dev: (2778.99, 2634.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15291) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 379, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 866) [M::mem_pestat] mean and std.dev: (396.69, 138.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1054) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (607, 1618, 4195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11371) [M::mem_pestat] mean and std.dev: (2687.98, 2634.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14959) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (607, 1618, 4236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11494) [M::mem_pestat] mean and std.dev: (2718.53, 2652.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15123) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 262.945 CPU sec, 16.734 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3591, 92019, 3367, 3382) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (689, 1718, 4170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11132) [M::mem_pestat] mean and std.dev: (2721.47, 2579.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14613) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 380, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861) [M::mem_pestat] mean and std.dev: (396.97, 137.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1047) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (581, 1570, 4087) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11099) [M::mem_pestat] mean and std.dev: (2628.59, 2605.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14605) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (635, 1563, 3953) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10589) [M::mem_pestat] mean and std.dev: (2661.68, 2610.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13907) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 261.139 CPU sec, 16.645 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3485, 91528, 3308, 3435) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (623, 1561, 4116) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11102) [M::mem_pestat] mean and std.dev: (2652.51, 2594.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14595) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (304, 380, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 856) [M::mem_pestat] mean and std.dev: (396.93, 137.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1040) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (638, 1685, 4176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11252) [M::mem_pestat] mean and std.dev: (2722.97, 2655.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14790) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (661, 1646, 4279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11515) [M::mem_pestat] mean and std.dev: (2756.19, 2661.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15133) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 261.878 CPU sec, 16.652 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3348, 91771, 3261, 3458) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (629, 1639, 4211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11375) [M::mem_pestat] mean and std.dev: (2759.24, 2674.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14957) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 380, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 863) [M::mem_pestat] mean and std.dev: (396.75, 137.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1050) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (623, 1739, 4409) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11981) [M::mem_pestat] mean and std.dev: (2757.67, 2623.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15767) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (633, 1663, 4147) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11175) [M::mem_pestat] mean and std.dev: (2716.27, 2621.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14689) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 262.922 CPU sec, 16.812 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3339, 92107, 3324, 3558) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (666, 1705, 4119) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11025) [M::mem_pestat] mean and std.dev: (2711.67, 2595.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14478) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 380, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 863) [M::mem_pestat] mean and std.dev: (396.45, 137.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1050) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (650, 1587, 3905) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10415) [M::mem_pestat] mean and std.dev: (2663.58, 2628.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13670) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (645, 1625, 4154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11172) [M::mem_pestat] mean and std.dev: (2720.84, 2628.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14681) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 258.636 CPU sec, 16.407 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3370, 92181, 3256, 3514) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (658, 1642, 4075) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10909) [M::mem_pestat] mean and std.dev: (2691.82, 2590.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14326) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 379, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 866) [M::mem_pestat] mean and std.dev: (396.79, 137.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1054) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (615, 1602, 4064) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10962) [M::mem_pestat] mean and std.dev: (2666.32, 2619.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14411) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (652, 1663, 4147) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11137) [M::mem_pestat] mean and std.dev: (2696.12, 2547.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14632) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 264.368 CPU sec, 16.814 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3384, 92664, 3361, 3513) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (640, 1680, 4205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11335) [M::mem_pestat] mean and std.dev: (2746.09, 2620.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14900) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 379, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 863) [M::mem_pestat] mean and std.dev: (397.01, 138.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1050) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (658, 1714, 4298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11578) [M::mem_pestat] mean and std.dev: (2774.39, 2652.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15218) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (667, 1714, 4328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11650) [M::mem_pestat] mean and std.dev: (2806.80, 2677.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15311) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 261.603 CPU sec, 16.659 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3417, 92228, 3280, 3399) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (620, 1684, 4182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11306) [M::mem_pestat] mean and std.dev: (2717.42, 2634.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14868) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 381, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864) [M::mem_pestat] mean and std.dev: (397.70, 138.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1051) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (648, 1701, 4250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11454) [M::mem_pestat] mean and std.dev: (2746.82, 2628.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15056) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (612, 1563, 4051) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10929) [M::mem_pestat] mean and std.dev: (2631.82, 2584.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14368) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 262.802 CPU sec, 16.727 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3362, 92129, 3127, 3436) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (640, 1696, 4033) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10819) [M::mem_pestat] mean and std.dev: (2673.55, 2581.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14212) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 380, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 863) [M::mem_pestat] mean and std.dev: (396.66, 138.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1050) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (610, 1711, 4217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11431) [M::mem_pestat] mean and std.dev: (2755.63, 2660.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15038) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (659, 1686, 4272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11498) [M::mem_pestat] mean and std.dev: (2745.06, 2606.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15111) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 262.257 CPU sec, 16.712 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3447, 92116, 3357, 3488) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (674, 1583, 3853) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10211) [M::mem_pestat] mean and std.dev: (2636.20, 2564.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13390) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 380, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 866) [M::mem_pestat] mean and std.dev: (396.87, 138.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1054) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (631, 1631, 4008) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10762) [M::mem_pestat] mean and std.dev: (2663.82, 2606.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14139) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (674, 1586, 4024) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10724) [M::mem_pestat] mean and std.dev: (2646.56, 2551.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14074) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 263.638 CPU sec, 16.874 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3417, 91821, 3370, 3559) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (645, 1593, 3976) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10638) [M::mem_pestat] mean and std.dev: (2633.99, 2545.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13969) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 379, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860) [M::mem_pestat] mean and std.dev: (396.48, 137.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1046) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (591, 1541, 4106) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11136) [M::mem_pestat] mean and std.dev: (2637.65, 2627.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14651) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (688, 1720, 4290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11494) [M::mem_pestat] mean and std.dev: (2775.02, 2606.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15096) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 265.075 CPU sec, 16.893 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3443, 91696, 3411, 3383) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (618, 1647, 4097) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11055) [M::mem_pestat] mean and std.dev: (2686.06, 2598.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14534) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 380, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861) [M::mem_pestat] mean and std.dev: (396.86, 138.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1047) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (654, 1689, 4206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11310) [M::mem_pestat] mean and std.dev: (2711.61, 2590.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14862) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (680, 1736, 4238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11354) [M::mem_pestat] mean and std.dev: (2754.64, 2615.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14912) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 263.811 CPU sec, 16.870 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3427, 92030, 3325, 3475) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (676, 1696, 4120) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11008) [M::mem_pestat] mean and std.dev: (2688.40, 2558.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14452) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 379, 487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 857) [M::mem_pestat] mean and std.dev: (395.31, 137.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1042) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (625, 1634, 4100) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11050) [M::mem_pestat] mean and std.dev: (2681.00, 2618.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14525) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (593, 1572, 3878) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10448) [M::mem_pestat] mean and std.dev: (2597.23, 2560.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13733) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 262.159 CPU sec, 16.760 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3515, 92521, 3246, 3560) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (711, 1711, 4104) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10890) [M::mem_pestat] mean and std.dev: (2720.44, 2564.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14283) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 379, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 863) [M::mem_pestat] mean and std.dev: (395.95, 137.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1050) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (610, 1635, 4231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11473) [M::mem_pestat] mean and std.dev: (2737.45, 2682.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15094) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (627, 1587, 4013) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10785) [M::mem_pestat] mean and std.dev: (2631.76, 2553.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14171) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 263.590 CPU sec, 16.779 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3344, 92268, 3256, 3492) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (637, 1662, 4164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11218) [M::mem_pestat] mean and std.dev: (2707.66, 2597.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14745) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 380, 487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 857) [M::mem_pestat] mean and std.dev: (395.88, 137.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1042) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (651, 1623, 4256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11466) [M::mem_pestat] mean and std.dev: (2741.53, 2652.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15071) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (657, 1665, 4371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11799) [M::mem_pestat] mean and std.dev: (2809.75, 2704.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15513) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 263.628 CPU sec, 16.699 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3338, 92127, 3305, 3438) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (612, 1571, 4131) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11169) [M::mem_pestat] mean and std.dev: (2677.88, 2614.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14688) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 379, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860) [M::mem_pestat] mean and std.dev: (396.64, 137.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1046) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (620, 1698, 4259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11537) [M::mem_pestat] mean and std.dev: (2745.19, 2631.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15176) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (635, 1746, 4252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11486) [M::mem_pestat] mean and std.dev: (2777.26, 2677.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15103) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 262.628 CPU sec, 16.710 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3432, 92557, 3332, 3461) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (609, 1633, 4187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11343) [M::mem_pestat] mean and std.dev: (2733.51, 2670.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14921) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 379, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860) [M::mem_pestat] mean and std.dev: (396.26, 138.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1046) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (622, 1747, 4291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11629) [M::mem_pestat] mean and std.dev: (2760.09, 2645.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15298) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (659, 1666, 3909) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10409) [M::mem_pestat] mean and std.dev: (2635.75, 2544.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13659) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 263.442 CPU sec, 16.842 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3549, 92223, 3189, 3486) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (617, 1588, 4078) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11000) [M::mem_pestat] mean and std.dev: (2650.48, 2598.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14461) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (301, 378, 487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 859) [M::mem_pestat] mean and std.dev: (395.13, 137.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1045) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (641, 1818, 4416) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11966) [M::mem_pestat] mean and std.dev: (2824.93, 2692.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15741) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (661, 1671, 3968) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10582) [M::mem_pestat] mean and std.dev: (2653.11, 2558.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13889) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 261.381 CPU sec, 16.547 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3541, 92233, 3453, 3523) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (606, 1608, 4119) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11145) [M::mem_pestat] mean and std.dev: (2674.21, 2620.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14658) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 379, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 863) [M::mem_pestat] mean and std.dev: (396.23, 137.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1050) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (628, 1641, 4065) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10939) [M::mem_pestat] mean and std.dev: (2648.61, 2576.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14376) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (647, 1638, 4133) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11105) [M::mem_pestat] mean and std.dev: (2697.15, 2599.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14591) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 261.913 CPU sec, 16.674 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3420, 91975, 3255, 3538) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (656, 1592, 3920) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10448) [M::mem_pestat] mean and std.dev: (2632.05, 2547.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13712) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (301, 378, 485) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 853) [M::mem_pestat] mean and std.dev: (394.49, 136.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1037) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (622, 1732, 4282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11602) [M::mem_pestat] mean and std.dev: (2736.62, 2630.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15262) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (619, 1559, 4124) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11134) [M::mem_pestat] mean and std.dev: (2649.40, 2593.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14639) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 264.660 CPU sec, 16.847 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3362, 92173, 3342, 3350) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (672, 1673, 4356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11724) [M::mem_pestat] mean and std.dev: (2775.44, 2657.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15408) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 379, 485) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 851) [M::mem_pestat] mean and std.dev: (394.87, 136.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1034) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (610, 1678, 4048) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10924) [M::mem_pestat] mean and std.dev: (2678.46, 2638.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14362) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (661, 1550, 3831) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10171) [M::mem_pestat] mean and std.dev: (2587.88, 2517.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13341) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 265.965 CPU sec, 16.930 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3487, 92575, 3327, 3418) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (616, 1529, 3996) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10756) [M::mem_pestat] mean and std.dev: (2609.63, 2567.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14136) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (301, 379, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 862) [M::mem_pestat] mean and std.dev: (396.14, 137.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1049) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (641, 1725, 4306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11636) [M::mem_pestat] mean and std.dev: (2725.49, 2625.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15301) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (655, 1728, 4367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11791) [M::mem_pestat] mean and std.dev: (2841.63, 2719.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15503) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 268.682 CPU sec, 17.126 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3407, 92778, 3430, 3374) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (618, 1521, 3709) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9891) [M::mem_pestat] mean and std.dev: (2571.99, 2559.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12982) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (301, 377, 484) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 850) [M::mem_pestat] mean and std.dev: (393.99, 136.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1033) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (616, 1646, 4196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11356) [M::mem_pestat] mean and std.dev: (2703.34, 2626.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14936) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (697, 1732, 4168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11110) [M::mem_pestat] mean and std.dev: (2761.80, 2603.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14581) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 263.566 CPU sec, 16.855 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3641, 93000, 3205, 3352) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (636, 1619, 3877) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10359) [M::mem_pestat] mean and std.dev: (2636.76, 2558.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13600) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (300, 378, 486) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 858) [M::mem_pestat] mean and std.dev: (394.27, 136.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1044) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (624, 1774, 4276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11580) [M::mem_pestat] mean and std.dev: (2775.48, 2660.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15232) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (626, 1623, 4158) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11222) [M::mem_pestat] mean and std.dev: (2671.41, 2571.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14754) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 262.843 CPU sec, 16.669 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3477, 93268, 3427, 3430) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (668, 1694, 4129) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11051) [M::mem_pestat] mean and std.dev: (2681.61, 2580.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14512) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (300, 378, 484) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 852) [M::mem_pestat] mean and std.dev: (393.10, 135.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1036) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (658, 1652, 4069) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10891) [M::mem_pestat] mean and std.dev: (2657.63, 2573.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14302) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (622, 1653, 4081) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10999) [M::mem_pestat] mean and std.dev: (2694.10, 2623.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14458) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 262.844 CPU sec, 16.834 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3425, 92964, 3287, 3481) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (682, 1550, 3937) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10447) [M::mem_pestat] mean and std.dev: (2660.30, 2595.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13702) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (301, 379, 486) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 856) [M::mem_pestat] mean and std.dev: (395.34, 137.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1041) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (603, 1600, 4055) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10959) [M::mem_pestat] mean and std.dev: (2635.40, 2581.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14411) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (631, 1651, 4102) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11044) [M::mem_pestat] mean and std.dev: (2715.94, 2600.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14515) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 264.268 CPU sec, 16.780 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3475, 92979, 3316, 3545) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (668, 1771, 4476) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12092) [M::mem_pestat] mean and std.dev: (2869.22, 2716.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15900) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (301, 378, 487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 859) [M::mem_pestat] mean and std.dev: (395.26, 137.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1045) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (631, 1728, 4309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11665) [M::mem_pestat] mean and std.dev: (2754.55, 2651.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15343) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (656, 1828, 4421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11951) [M::mem_pestat] mean and std.dev: (2838.70, 2676.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15716) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 263.016 CPU sec, 16.859 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3539, 92993, 3241, 3455) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (669, 1746, 4095) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10947) [M::mem_pestat] mean and std.dev: (2722.84, 2563.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14373) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 379, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860) [M::mem_pestat] mean and std.dev: (395.85, 138.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1046) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (639, 1684, 4177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11253) [M::mem_pestat] mean and std.dev: (2755.47, 2691.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14791) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (610, 1595, 3845) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10315) [M::mem_pestat] mean and std.dev: (2615.93, 2560.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13550) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 263.358 CPU sec, 16.744 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3400, 92306, 3279, 3314) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (681, 1747, 4165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11133) [M::mem_pestat] mean and std.dev: (2754.48, 2623.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14617) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 379, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860) [M::mem_pestat] mean and std.dev: (395.77, 137.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1046) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (604, 1716, 4113) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11131) [M::mem_pestat] mean and std.dev: (2709.57, 2632.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14640) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (616, 1546, 4083) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11017) [M::mem_pestat] mean and std.dev: (2638.63, 2608.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14484) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 260.594 CPU sec, 16.776 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3369, 93145, 3403, 3440) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (674, 1710, 4199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11249) [M::mem_pestat] mean and std.dev: (2728.29, 2570.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14774) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 380, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864) [M::mem_pestat] mean and std.dev: (397.46, 137.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1051) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (603, 1657, 3988) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10758) [M::mem_pestat] mean and std.dev: (2653.04, 2589.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14143) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (653, 1626, 4127) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11075) [M::mem_pestat] mean and std.dev: (2675.93, 2583.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14549) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 262.907 CPU sec, 16.684 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3379, 92421, 3330, 3419) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (646, 1576, 4086) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10966) [M::mem_pestat] mean and std.dev: (2703.74, 2633.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14406) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 380, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864) [M::mem_pestat] mean and std.dev: (397.28, 137.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1051) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (615, 1722, 4015) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10815) [M::mem_pestat] mean and std.dev: (2655.88, 2538.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14215) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (630, 1633, 3996) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10728) [M::mem_pestat] mean and std.dev: (2676.07, 2587.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14094) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 262.922 CPU sec, 16.734 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3537, 92824, 3450, 3329) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (646, 1628, 4130) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11098) [M::mem_pestat] mean and std.dev: (2713.42, 2617.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14582) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (301, 379, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 865) [M::mem_pestat] mean and std.dev: (396.50, 138.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1053) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (639, 1760, 4274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11544) [M::mem_pestat] mean and std.dev: (2744.68, 2620.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15179) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (618, 1621, 4218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11418) [M::mem_pestat] mean and std.dev: (2711.29, 2632.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15018) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 261.710 CPU sec, 16.648 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3382, 92180, 3359, 3565) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (688, 1706, 4188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11188) [M::mem_pestat] mean and std.dev: (2749.38, 2620.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14688) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (301, 379, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 862) [M::mem_pestat] mean and std.dev: (395.73, 137.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1049) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (655, 1756, 4375) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11815) [M::mem_pestat] mean and std.dev: (2772.70, 2637.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15535) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (656, 1697, 4182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11234) [M::mem_pestat] mean and std.dev: (2734.98, 2615.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14760) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 260.823 CPU sec, 16.803 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3473, 91848, 3324, 3399) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (608, 1471, 3846) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10322) [M::mem_pestat] mean and std.dev: (2588.13, 2579.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13560) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 381, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 866) [M::mem_pestat] mean and std.dev: (397.56, 138.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1054) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (625, 1705, 4295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11635) [M::mem_pestat] mean and std.dev: (2752.81, 2640.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15305) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (654, 1589, 4110) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11022) [M::mem_pestat] mean and std.dev: (2684.17, 2605.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14478) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 261.015 CPU sec, 16.588 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3447, 91554, 3416, 3402) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (675, 1677, 4122) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11016) [M::mem_pestat] mean and std.dev: (2756.76, 2634.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14463) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 381, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864) [M::mem_pestat] mean and std.dev: (397.45, 137.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1051) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (664, 1740, 4217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11323) [M::mem_pestat] mean and std.dev: (2744.24, 2602.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14876) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (683, 1759, 4089) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10901) [M::mem_pestat] mean and std.dev: (2742.14, 2603.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14307) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 260.132 CPU sec, 16.650 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3561, 92236, 3207, 3390) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (649, 1682, 4031) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10795) [M::mem_pestat] mean and std.dev: (2690.59, 2570.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14177) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 380, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 866) [M::mem_pestat] mean and std.dev: (397.63, 138.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1054) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (657, 1720, 4015) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10731) [M::mem_pestat] mean and std.dev: (2705.30, 2612.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14089) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (656, 1557, 4076) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10916) [M::mem_pestat] mean and std.dev: (2646.88, 2563.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14336) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 260.395 CPU sec, 16.523 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3347, 93026, 3290, 3467) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (633, 1691, 4185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11289) [M::mem_pestat] mean and std.dev: (2733.62, 2619.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14841) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 380, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 866) [M::mem_pestat] mean and std.dev: (397.72, 138.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1054) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (617, 1658, 4259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11543) [M::mem_pestat] mean and std.dev: (2726.52, 2658.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15185) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (621, 1518, 4053) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10917) [M::mem_pestat] mean and std.dev: (2628.34, 2560.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14349) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 258.823 CPU sec, 16.504 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3477, 92467, 3341, 3506) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (636, 1633, 4145) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11163) [M::mem_pestat] mean and std.dev: (2672.69, 2562.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14672) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 381, 492) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 870) [M::mem_pestat] mean and std.dev: (398.49, 139.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1059) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (581, 1662, 4044) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10970) [M::mem_pestat] mean and std.dev: (2636.67, 2593.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14433) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (687, 1776, 4379) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11763) [M::mem_pestat] mean and std.dev: (2830.49, 2679.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15455) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 262.873 CPU sec, 16.869 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3506, 92250, 3358, 3438) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (638, 1698, 4285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11579) [M::mem_pestat] mean and std.dev: (2755.28, 2638.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15226) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 380, 492) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 870) [M::mem_pestat] mean and std.dev: (397.77, 139.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1059) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (648, 1813, 4469) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12111) [M::mem_pestat] mean and std.dev: (2843.98, 2690.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15932) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (620, 1583, 4052) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10916) [M::mem_pestat] mean and std.dev: (2705.40, 2641.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14348) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 260.208 CPU sec, 16.508 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3407, 91872, 3263, 3397) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (663, 1655, 4023) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10743) [M::mem_pestat] mean and std.dev: (2686.56, 2586.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14103) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 379, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 858) [M::mem_pestat] mean and std.dev: (396.73, 137.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1043) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (599, 1674, 4077) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11033) [M::mem_pestat] mean and std.dev: (2708.25, 2632.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14511) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (634, 1565, 4007) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10753) [M::mem_pestat] mean and std.dev: (2636.60, 2571.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14126) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 261.429 CPU sec, 16.652 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3483, 91633, 3359, 3419) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (658, 1605, 4292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11560) [M::mem_pestat] mean and std.dev: (2776.36, 2694.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15194) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 381, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 867) [M::mem_pestat] mean and std.dev: (398.60, 139.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1055) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (621, 1778, 4249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11505) [M::mem_pestat] mean and std.dev: (2759.69, 2637.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15133) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (618, 1583, 3940) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10584) [M::mem_pestat] mean and std.dev: (2636.57, 2589.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13906) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 258.435 CPU sec, 16.658 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3505, 92043, 3180, 3409) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (682, 1789, 4212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11272) [M::mem_pestat] mean and std.dev: (2755.21, 2597.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14802) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 381, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861) [M::mem_pestat] mean and std.dev: (397.31, 137.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1047) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (644, 1638, 4085) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10967) [M::mem_pestat] mean and std.dev: (2655.31, 2564.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14408) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (596, 1537, 4164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11300) [M::mem_pestat] mean and std.dev: (2669.62, 2617.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14868) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 259.558 CPU sec, 16.473 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3435, 92235, 3366, 3483) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (646, 1631, 4225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11383) [M::mem_pestat] mean and std.dev: (2750.18, 2662.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14962) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (304, 382, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 865) [M::mem_pestat] mean and std.dev: (398.79, 138.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1052) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (666, 1855, 4543) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12297) [M::mem_pestat] mean and std.dev: (2868.84, 2715.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16174) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (645, 1660, 4078) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10944) [M::mem_pestat] mean and std.dev: (2696.37, 2615.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14377) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 261.972 CPU sec, 16.674 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3414, 91754, 3388, 3474) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (618, 1664, 4096) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11052) [M::mem_pestat] mean and std.dev: (2688.57, 2591.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14530) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 381, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 867) [M::mem_pestat] mean and std.dev: (398.56, 139.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1055) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (626, 1789, 4321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11711) [M::mem_pestat] mean and std.dev: (2765.14, 2635.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15406) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (629, 1608, 4144) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11174) [M::mem_pestat] mean and std.dev: (2676.23, 2589.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14689) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 263.131 CPU sec, 16.904 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3494, 92795, 3365, 3529) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (671, 1620, 4014) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10700) [M::mem_pestat] mean and std.dev: (2665.90, 2560.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14043) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (304, 381, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 865) [M::mem_pestat] mean and std.dev: (398.18, 137.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1052) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (659, 1667, 4258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11456) [M::mem_pestat] mean and std.dev: (2695.83, 2588.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15055) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (676, 1615, 3876) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10276) [M::mem_pestat] mean and std.dev: (2621.67, 2505.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13476) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 262.318 CPU sec, 16.640 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3336, 92356, 3445, 3402) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (666, 1729, 4147) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11109) [M::mem_pestat] mean and std.dev: (2746.99, 2620.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14590) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (304, 382, 492) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 868) [M::mem_pestat] mean and std.dev: (399.08, 138.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1056) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (633, 1723, 4098) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11028) [M::mem_pestat] mean and std.dev: (2707.62, 2613.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14493) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (655, 1735, 4151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11143) [M::mem_pestat] mean and std.dev: (2728.90, 2594.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14639) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 262.153 CPU sec, 16.923 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3412, 92243, 3198, 3382) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (655, 1625, 4387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11851) [M::mem_pestat] mean and std.dev: (2798.39, 2712.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15583) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 380, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 866) [M::mem_pestat] mean and std.dev: (397.25, 138.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1054) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (619, 1735, 4256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11530) [M::mem_pestat] mean and std.dev: (2756.95, 2647.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15167) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (652, 1614, 4182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11242) [M::mem_pestat] mean and std.dev: (2725.45, 2637.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14772) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 264.211 CPU sec, 16.747 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3416, 91750, 3326, 3458) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (680, 1631, 4232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11336) [M::mem_pestat] mean and std.dev: (2711.93, 2582.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14888) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 380, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 867) [M::mem_pestat] mean and std.dev: (398.41, 139.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1055) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (633, 1698, 4259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11511) [M::mem_pestat] mean and std.dev: (2743.56, 2671.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15137) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (662, 1710, 4265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11471) [M::mem_pestat] mean and std.dev: (2746.16, 2612.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15074) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 262.787 CPU sec, 16.809 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3403, 92199, 3306, 3314) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (620, 1562, 4202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11366) [M::mem_pestat] mean and std.dev: (2700.07, 2618.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14948) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 382, 492) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 870) [M::mem_pestat] mean and std.dev: (398.76, 139.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1059) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (654, 1722, 4298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11586) [M::mem_pestat] mean and std.dev: (2749.23, 2656.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15230) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (635, 1658, 4058) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10904) [M::mem_pestat] mean and std.dev: (2686.27, 2577.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14327) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 262.296 CPU sec, 16.667 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3459, 92430, 3278, 3476) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (637, 1646, 4143) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11155) [M::mem_pestat] mean and std.dev: (2709.45, 2631.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14661) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 380, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 863) [M::mem_pestat] mean and std.dev: (397.34, 138.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1050) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (607, 1649, 3944) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10618) [M::mem_pestat] mean and std.dev: (2645.15, 2604.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13955) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (636, 1651, 4110) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11058) [M::mem_pestat] mean and std.dev: (2718.17, 2635.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14532) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 261.439 CPU sec, 16.730 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3438, 91963, 3341, 3461) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (667, 1636, 4150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11116) [M::mem_pestat] mean and std.dev: (2692.25, 2598.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14599) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 380, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864) [M::mem_pestat] mean and std.dev: (397.80, 138.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1051) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (576, 1574, 4051) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11001) [M::mem_pestat] mean and std.dev: (2639.19, 2592.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14476) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (599, 1608, 3969) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10709) [M::mem_pestat] mean and std.dev: (2633.69, 2564.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14079) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 262.219 CPU sec, 16.669 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3381, 92460, 3250, 3493) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (680, 1748, 4140) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11060) [M::mem_pestat] mean and std.dev: (2740.82, 2561.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14520) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 381, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864) [M::mem_pestat] mean and std.dev: (397.57, 138.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1051) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (620, 1626, 4162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11246) [M::mem_pestat] mean and std.dev: (2689.38, 2617.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14788) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (630, 1564, 3922) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10506) [M::mem_pestat] mean and std.dev: (2618.95, 2585.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13798) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 259.569 CPU sec, 16.602 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3439, 92197, 3183, 3388) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (666, 1724, 4276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11496) [M::mem_pestat] mean and std.dev: (2774.51, 2627.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15106) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 381, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864) [M::mem_pestat] mean and std.dev: (398.03, 138.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1051) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (630, 1660, 4267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11541) [M::mem_pestat] mean and std.dev: (2735.21, 2664.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15178) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (597, 1587, 4055) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10971) [M::mem_pestat] mean and std.dev: (2661.46, 2604.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14429) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 262.805 CPU sec, 16.650 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3442, 91720, 3267, 3400) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (684, 1780, 4307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11553) [M::mem_pestat] mean and std.dev: (2796.99, 2619.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15176) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 382, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 867) [M::mem_pestat] mean and std.dev: (398.31, 138.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1055) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (600, 1734, 4312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11736) [M::mem_pestat] mean and std.dev: (2739.08, 2659.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15448) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (595, 1544, 4073) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11029) [M::mem_pestat] mean and std.dev: (2634.97, 2612.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14507) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 263.738 CPU sec, 16.818 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3311, 92147, 3345, 3504) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (653, 1665, 4030) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10784) [M::mem_pestat] mean and std.dev: (2698.09, 2588.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14161) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 380, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 863) [M::mem_pestat] mean and std.dev: (397.54, 138.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1050) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (631, 1708, 4318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11692) [M::mem_pestat] mean and std.dev: (2774.21, 2664.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15379) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (675, 1707, 4012) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10686) [M::mem_pestat] mean and std.dev: (2682.37, 2521.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14023) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 263.355 CPU sec, 16.850 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3492, 91941, 3379, 3571) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (641, 1709, 4200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11318) [M::mem_pestat] mean and std.dev: (2734.30, 2608.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14877) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 380, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 863) [M::mem_pestat] mean and std.dev: (396.84, 138.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1050) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (638, 1713, 4394) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11906) [M::mem_pestat] mean and std.dev: (2799.88, 2691.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15662) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (634, 1679, 4054) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10894) [M::mem_pestat] mean and std.dev: (2685.63, 2596.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14314) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 260.809 CPU sec, 16.524 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3323, 91995, 3338, 3427) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (671, 1622, 4048) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10802) [M::mem_pestat] mean and std.dev: (2684.33, 2569.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14179) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 381, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864) [M::mem_pestat] mean and std.dev: (397.80, 137.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1051) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (623, 1699, 4189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11321) [M::mem_pestat] mean and std.dev: (2731.61, 2645.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14887) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (641, 1633, 4186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11276) [M::mem_pestat] mean and std.dev: (2739.27, 2674.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14821) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 263.677 CPU sec, 16.857 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3469, 92285, 3176, 3386) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (611, 1539, 3927) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10559) [M::mem_pestat] mean and std.dev: (2615.88, 2584.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13875) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 381, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 858) [M::mem_pestat] mean and std.dev: (397.26, 137.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1043) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (618, 1613, 3937) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10575) [M::mem_pestat] mean and std.dev: (2609.03, 2561.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13894) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (659, 1635, 4272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11498) [M::mem_pestat] mean and std.dev: (2754.96, 2664.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15111) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 262.739 CPU sec, 16.663 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3474, 91973, 3366, 3407) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (680, 1704, 4198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11234) [M::mem_pestat] mean and std.dev: (2723.06, 2561.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14752) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 380, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 866) [M::mem_pestat] mean and std.dev: (396.80, 138.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1054) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (618, 1696, 4095) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11049) [M::mem_pestat] mean and std.dev: (2685.17, 2600.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14526) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (611, 1544, 4010) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10808) [M::mem_pestat] mean and std.dev: (2636.62, 2601.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14207) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 261.020 CPU sec, 16.609 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3468, 91709, 3298, 3360) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (672, 1583, 4076) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10884) [M::mem_pestat] mean and std.dev: (2680.44, 2597.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14288) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (304, 381, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 862) [M::mem_pestat] mean and std.dev: (397.80, 137.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1048) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (596, 1689, 4209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11435) [M::mem_pestat] mean and std.dev: (2689.15, 2617.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15048) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (658, 1636, 4370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11794) [M::mem_pestat] mean and std.dev: (2764.93, 2663.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15506) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 262.714 CPU sec, 17.493 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3569, 91616, 3285, 3415) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (675, 1664, 4003) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10659) [M::mem_pestat] mean and std.dev: (2672.56, 2553.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13987) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 379, 487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 857) [M::mem_pestat] mean and std.dev: (395.71, 137.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1042) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (614, 1672, 4198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11366) [M::mem_pestat] mean and std.dev: (2714.88, 2621.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14950) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (629, 1584, 4104) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11054) [M::mem_pestat] mean and std.dev: (2654.00, 2574.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14529) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 264.594 CPU sec, 16.799 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3442, 92428, 3276, 3522) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (618, 1636, 3988) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10728) [M::mem_pestat] mean and std.dev: (2633.43, 2538.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14098) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 381, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 867) [M::mem_pestat] mean and std.dev: (398.13, 138.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1055) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (630, 1701, 4168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11244) [M::mem_pestat] mean and std.dev: (2758.12, 2662.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14782) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (655, 1723, 4161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11173) [M::mem_pestat] mean and std.dev: (2700.57, 2562.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14679) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 261.944 CPU sec, 16.728 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3463, 92003, 3360, 3401) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (641, 1643, 3973) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10637) [M::mem_pestat] mean and std.dev: (2638.97, 2547.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13969) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 380, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 866) [M::mem_pestat] mean and std.dev: (396.91, 137.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1054) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (625, 1714, 4263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11539) [M::mem_pestat] mean and std.dev: (2747.39, 2628.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15177) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (672, 1766, 4095) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10941) [M::mem_pestat] mean and std.dev: (2746.46, 2612.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14364) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 261.857 CPU sec, 16.600 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3428, 92113, 3320, 3382) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (644, 1620, 3937) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10523) [M::mem_pestat] mean and std.dev: (2666.98, 2607.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13816) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 381, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861) [M::mem_pestat] mean and std.dev: (397.67, 138.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1047) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (595, 1580, 4137) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11221) [M::mem_pestat] mean and std.dev: (2667.82, 2618.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14763) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (601, 1610, 3910) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10528) [M::mem_pestat] mean and std.dev: (2626.06, 2558.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13837) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 262.860 CPU sec, 17.060 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3406, 92159, 3271, 3414) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (645, 1672, 4331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11703) [M::mem_pestat] mean and std.dev: (2781.82, 2674.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15389) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 380, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861) [M::mem_pestat] mean and std.dev: (397.16, 137.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1047) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (597, 1650, 4259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11583) [M::mem_pestat] mean and std.dev: (2721.24, 2644.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15245) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (617, 1602, 4024) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10838) [M::mem_pestat] mean and std.dev: (2635.03, 2569.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14245) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 262.787 CPU sec, 16.636 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3415, 92045, 3124, 3592) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (646, 1677, 4106) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11026) [M::mem_pestat] mean and std.dev: (2718.15, 2612.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14486) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 380, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860) [M::mem_pestat] mean and std.dev: (396.08, 136.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1046) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (637, 1714, 4269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11533) [M::mem_pestat] mean and std.dev: (2739.02, 2656.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15165) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (638, 1567, 4240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11444) [M::mem_pestat] mean and std.dev: (2710.38, 2635.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15046) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 262.791 CPU sec, 16.928 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3461, 92546, 3217, 3310) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (645, 1632, 4052) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10866) [M::mem_pestat] mean and std.dev: (2687.85, 2602.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14273) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 379, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 863) [M::mem_pestat] mean and std.dev: (396.58, 139.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1050) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (634, 1771, 4394) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11914) [M::mem_pestat] mean and std.dev: (2811.92, 2697.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15674) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (685, 1675, 4012) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10666) [M::mem_pestat] mean and std.dev: (2673.93, 2570.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13993) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 264.149 CPU sec, 16.774 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3378, 92768, 3262, 3436) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (621, 1577, 3921) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10521) [M::mem_pestat] mean and std.dev: (2611.27, 2552.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13821) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (301, 378, 487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 859) [M::mem_pestat] mean and std.dev: (395.20, 137.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1045) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (647, 1711, 4366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11804) [M::mem_pestat] mean and std.dev: (2781.58, 2651.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15523) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (642, 1595, 4033) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10815) [M::mem_pestat] mean and std.dev: (2669.96, 2577.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14206) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 264.303 CPU sec, 16.802 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3469, 92064, 3272, 3421) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (696, 1677, 4156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11076) [M::mem_pestat] mean and std.dev: (2719.70, 2589.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14536) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (301, 380, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 865) [M::mem_pestat] mean and std.dev: (397.08, 139.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1053) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (590, 1539, 3867) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10421) [M::mem_pestat] mean and std.dev: (2590.74, 2574.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13698) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (650, 1675, 4030) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10790) [M::mem_pestat] mean and std.dev: (2679.30, 2592.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14170) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 265.329 CPU sec, 17.111 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3372, 92645, 3249, 3437) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (650, 1561, 3874) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10322) [M::mem_pestat] mean and std.dev: (2635.10, 2580.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13546) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 380, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860) [M::mem_pestat] mean and std.dev: (396.16, 137.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1046) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (607, 1676, 4142) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11212) [M::mem_pestat] mean and std.dev: (2687.02, 2606.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14747) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (639, 1707, 4219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11379) [M::mem_pestat] mean and std.dev: (2746.74, 2659.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14959) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 263.626 CPU sec, 16.774 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3506, 92592, 3291, 3339) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (625, 1529, 4122) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11116) [M::mem_pestat] mean and std.dev: (2636.74, 2575.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14613) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 381, 492) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 872) [M::mem_pestat] mean and std.dev: (398.37, 139.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1062) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (631, 1638, 4196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11326) [M::mem_pestat] mean and std.dev: (2660.69, 2567.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14891) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (647, 1583, 3990) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10676) [M::mem_pestat] mean and std.dev: (2647.87, 2565.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14019) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 267.067 CPU sec, 17.156 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3466, 92226, 3224, 3470) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (636, 1618, 4118) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11082) [M::mem_pestat] mean and std.dev: (2680.57, 2599.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14564) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 380, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864) [M::mem_pestat] mean and std.dev: (397.48, 138.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1051) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (606, 1690, 4331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11781) [M::mem_pestat] mean and std.dev: (2773.31, 2690.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15506) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (656, 1679, 4062) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10874) [M::mem_pestat] mean and std.dev: (2697.69, 2586.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14280) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 263.234 CPU sec, 16.808 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3461, 92407, 3212, 3312) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (671, 1722, 3920) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10418) [M::mem_pestat] mean and std.dev: (2656.01, 2516.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13667) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 379, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860) [M::mem_pestat] mean and std.dev: (396.62, 137.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1046) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (682, 1743, 4373) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11755) [M::mem_pestat] mean and std.dev: (2805.61, 2673.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15446) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (666, 1634, 4066) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10866) [M::mem_pestat] mean and std.dev: (2680.31, 2574.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14266) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 265.400 CPU sec, 16.909 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3520, 92211, 3341, 3400) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (658, 1645, 4045) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10819) [M::mem_pestat] mean and std.dev: (2719.79, 2629.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14206) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 379, 487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 857) [M::mem_pestat] mean and std.dev: (395.17, 136.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1042) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (661, 1661, 4177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11209) [M::mem_pestat] mean and std.dev: (2729.59, 2635.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14725) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (665, 1595, 3984) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10622) [M::mem_pestat] mean and std.dev: (2657.99, 2583.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13941) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 266.163 CPU sec, 17.089 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3438, 92361, 3296, 3418) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (646, 1683, 4149) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11155) [M::mem_pestat] mean and std.dev: (2746.34, 2647.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14658) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 380, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864) [M::mem_pestat] mean and std.dev: (396.70, 138.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1051) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (609, 1679, 4161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11265) [M::mem_pestat] mean and std.dev: (2685.03, 2610.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14817) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (639, 1717, 4511) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12255) [M::mem_pestat] mean and std.dev: (2850.89, 2722.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16127) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 264.875 CPU sec, 16.847 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3458, 92195, 3274, 3382) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (625, 1647, 4053) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10909) [M::mem_pestat] mean and std.dev: (2717.81, 2643.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14337) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (301, 379, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 865) [M::mem_pestat] mean and std.dev: (396.14, 137.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1053) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (604, 1606, 3872) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10408) [M::mem_pestat] mean and std.dev: (2582.27, 2543.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13676) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (664, 1780, 4366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11770) [M::mem_pestat] mean and std.dev: (2825.53, 2677.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15472) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 266.080 CPU sec, 16.972 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3379, 92506, 3376, 3490) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (641, 1615, 4004) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10730) [M::mem_pestat] mean and std.dev: (2654.04, 2549.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14093) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (301, 378, 487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 859) [M::mem_pestat] mean and std.dev: (394.95, 137.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1045) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (615, 1613, 4163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11259) [M::mem_pestat] mean and std.dev: (2661.74, 2603.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14807) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (667, 1693, 4327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11647) [M::mem_pestat] mean and std.dev: (2740.32, 2609.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15307) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 265.302 CPU sec, 16.953 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3471, 91995, 3156, 3423) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (611, 1548, 4102) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11084) [M::mem_pestat] mean and std.dev: (2675.49, 2640.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14575) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (301, 379, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 862) [M::mem_pestat] mean and std.dev: (396.00, 137.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1049) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (613, 1557, 3950) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10624) [M::mem_pestat] mean and std.dev: (2613.44, 2598.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13961) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (664, 1684, 4135) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11077) [M::mem_pestat] mean and std.dev: (2755.57, 2650.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14548) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 263.264 CPU sec, 16.683 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3401, 92288, 3292, 3424) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (656, 1619, 3954) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10550) [M::mem_pestat] mean and std.dev: (2648.36, 2558.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13848) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 379, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 863) [M::mem_pestat] mean and std.dev: (395.57, 137.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1050) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (635, 1703, 4124) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11102) [M::mem_pestat] mean and std.dev: (2710.07, 2629.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14591) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (640, 1664, 4123) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11089) [M::mem_pestat] mean and std.dev: (2697.95, 2608.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14572) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 266.040 CPU sec, 16.941 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3467, 92897, 3279, 3405) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (723, 1777, 4374) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11676) [M::mem_pestat] mean and std.dev: (2822.28, 2640.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15327) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (300, 378, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864) [M::mem_pestat] mean and std.dev: (395.25, 137.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1052) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (629, 1673, 4283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11591) [M::mem_pestat] mean and std.dev: (2747.21, 2666.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15245) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (665, 1677, 4201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11273) [M::mem_pestat] mean and std.dev: (2693.59, 2557.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14809) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 266.877 CPU sec, 17.047 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3488, 92123, 3359, 3380) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (652, 1757, 4208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11320) [M::mem_pestat] mean and std.dev: (2764.28, 2628.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14876) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 379, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860) [M::mem_pestat] mean and std.dev: (395.74, 137.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1046) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (601, 1661, 4170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11308) [M::mem_pestat] mean and std.dev: (2660.70, 2547.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14877) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (648, 1622, 4106) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11022) [M::mem_pestat] mean and std.dev: (2704.06, 2639.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14480) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 265.500 CPU sec, 17.072 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3428, 92483, 3292, 3568) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (692, 1698, 4213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11255) [M::mem_pestat] mean and std.dev: (2773.84, 2625.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14776) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (301, 379, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 862) [M::mem_pestat] mean and std.dev: (395.91, 138.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1049) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (631, 1695, 4094) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11020) [M::mem_pestat] mean and std.dev: (2711.66, 2606.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14483) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (658, 1715, 4168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11188) [M::mem_pestat] mean and std.dev: (2739.70, 2608.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14698) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 266.718 CPU sec, 17.018 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3437, 92572, 3286, 3497) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (642, 1633, 4009) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10743) [M::mem_pestat] mean and std.dev: (2649.49, 2558.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14110) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (301, 377, 486) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 856) [M::mem_pestat] mean and std.dev: (394.28, 137.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1041) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (660, 1742, 4214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11322) [M::mem_pestat] mean and std.dev: (2771.90, 2650.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14876) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (676, 1752, 4339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11665) [M::mem_pestat] mean and std.dev: (2810.09, 2659.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15328) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 266.856 CPU sec, 16.941 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3488, 92002, 3329, 3495) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (686, 1707, 4078) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10862) [M::mem_pestat] mean and std.dev: (2711.00, 2582.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14254) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (300, 377, 486) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 858) [M::mem_pestat] mean and std.dev: (394.66, 137.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1044) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (643, 1673, 4145) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11149) [M::mem_pestat] mean and std.dev: (2692.10, 2613.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14651) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (657, 1613, 4046) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10824) [M::mem_pestat] mean and std.dev: (2654.36, 2540.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14213) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 266.618 CPU sec, 17.128 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3523, 93211, 3222, 3435) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (660, 1636, 4130) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11070) [M::mem_pestat] mean and std.dev: (2700.94, 2592.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14540) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (301, 378, 485) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 853) [M::mem_pestat] mean and std.dev: (394.23, 136.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1037) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (670, 1842, 4512) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12196) [M::mem_pestat] mean and std.dev: (2828.18, 2642.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16038) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (653, 1717, 4251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11447) [M::mem_pestat] mean and std.dev: (2737.37, 2602.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15045) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 260.844 CPU sec, 16.594 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3392, 93580, 3191, 3341) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (634, 1599, 4077) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10963) [M::mem_pestat] mean and std.dev: (2693.21, 2636.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14406) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 379, 487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 857) [M::mem_pestat] mean and std.dev: (395.50, 137.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1042) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (573, 1701, 4052) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11010) [M::mem_pestat] mean and std.dev: (2678.30, 2593.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14489) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (652, 1728, 4182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11242) [M::mem_pestat] mean and std.dev: (2713.99, 2553.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14772) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 261.210 CPU sec, 16.828 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3578, 93194, 3225, 3627) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (641, 1723, 3981) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10661) [M::mem_pestat] mean and std.dev: (2691.80, 2568.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14001) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (301, 379, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 862) [M::mem_pestat] mean and std.dev: (395.89, 137.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1049) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (617, 1691, 4367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11867) [M::mem_pestat] mean and std.dev: (2768.26, 2670.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15617) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (611, 1675, 4225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11453) [M::mem_pestat] mean and std.dev: (2725.14, 2632.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15067) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 263.095 CPU sec, 16.722 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3506, 92626, 3310, 3558) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (664, 1690, 4030) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10762) [M::mem_pestat] mean and std.dev: (2710.38, 2579.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14128) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 379, 487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 857) [M::mem_pestat] mean and std.dev: (395.29, 137.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1042) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (645, 1770, 4358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11784) [M::mem_pestat] mean and std.dev: (2778.87, 2641.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15497) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (616, 1598, 3994) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10750) [M::mem_pestat] mean and std.dev: (2642.79, 2598.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14128) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 263.447 CPU sec, 16.949 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3268, 93089, 3276, 3470) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (653, 1596, 3937) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10505) [M::mem_pestat] mean and std.dev: (2654.97, 2575.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13789) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 380, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 863) [M::mem_pestat] mean and std.dev: (396.63, 138.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1050) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (619, 1661, 4316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11710) [M::mem_pestat] mean and std.dev: (2792.22, 2711.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15407) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (668, 1672, 4236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11372) [M::mem_pestat] mean and std.dev: (2732.96, 2589.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14940) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 261.554 CPU sec, 16.583 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3585, 93089, 3282, 3399) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (640, 1586, 4084) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10972) [M::mem_pestat] mean and std.dev: (2647.55, 2566.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14416) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (301, 380, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 862) [M::mem_pestat] mean and std.dev: (396.29, 137.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1049) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (640, 1700, 4113) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11059) [M::mem_pestat] mean and std.dev: (2716.59, 2605.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14532) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (624, 1617, 4227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11433) [M::mem_pestat] mean and std.dev: (2705.71, 2611.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15036) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 262.726 CPU sec, 16.700 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3364, 92881, 3311, 3514) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (632, 1586, 3963) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10625) [M::mem_pestat] mean and std.dev: (2656.86, 2604.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13956) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 380, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860) [M::mem_pestat] mean and std.dev: (396.28, 137.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1046) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (612, 1617, 4034) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10878) [M::mem_pestat] mean and std.dev: (2642.85, 2581.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14300) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (677, 1690, 4110) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10976) [M::mem_pestat] mean and std.dev: (2706.80, 2587.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14409) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 260.927 CPU sec, 16.598 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3503, 92288, 3449, 3639) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (636, 1662, 4414) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11970) [M::mem_pestat] mean and std.dev: (2784.56, 2679.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15748) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 379, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 863) [M::mem_pestat] mean and std.dev: (396.63, 138.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1050) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (626, 1654, 4257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11519) [M::mem_pestat] mean and std.dev: (2714.50, 2637.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15150) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (678, 1731, 4153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11103) [M::mem_pestat] mean and std.dev: (2735.19, 2591.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14578) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 262.476 CPU sec, 16.719 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3441, 92366, 3416, 3570) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (634, 1705, 4109) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11059) [M::mem_pestat] mean and std.dev: (2713.99, 2580.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14534) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 381, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 866) [M::mem_pestat] mean and std.dev: (396.70, 138.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1054) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (623, 1758, 4260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11534) [M::mem_pestat] mean and std.dev: (2780.42, 2672.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15171) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (635, 1673, 4373) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11849) [M::mem_pestat] mean and std.dev: (2772.69, 2687.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15587) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 261.013 CPU sec, 16.630 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3460, 92612, 3282, 3475) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (604, 1518, 3898) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10486) [M::mem_pestat] mean and std.dev: (2610.13, 2623.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13780) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 380, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 866) [M::mem_pestat] mean and std.dev: (397.36, 138.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1054) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (618, 1693, 4290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11634) [M::mem_pestat] mean and std.dev: (2733.89, 2659.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15306) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (677, 1677, 4085) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10901) [M::mem_pestat] mean and std.dev: (2679.19, 2520.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14309) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 261.445 CPU sec, 16.635 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3363, 92195, 3347, 3567) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (641, 1659, 4126) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11096) [M::mem_pestat] mean and std.dev: (2749.36, 2667.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14581) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 380, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 866) [M::mem_pestat] mean and std.dev: (397.65, 138.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1054) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (618, 1650, 4288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11628) [M::mem_pestat] mean and std.dev: (2740.06, 2653.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15298) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (657, 1661, 4035) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10791) [M::mem_pestat] mean and std.dev: (2685.98, 2566.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14169) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 261.735 CPU sec, 16.706 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3407, 92457, 3312, 3536) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (642, 1687, 4164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11208) [M::mem_pestat] mean and std.dev: (2724.70, 2611.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14730) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 380, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 863) [M::mem_pestat] mean and std.dev: (396.76, 137.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1050) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (676, 1783, 4209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11275) [M::mem_pestat] mean and std.dev: (2743.31, 2597.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14808) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (653, 1523, 4073) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10913) [M::mem_pestat] mean and std.dev: (2687.81, 2665.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14333) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 260.516 CPU sec, 16.568 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3517, 92230, 3260, 3459) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (626, 1533, 4022) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10814) [M::mem_pestat] mean and std.dev: (2647.54, 2602.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14210) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (304, 382, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 862) [M::mem_pestat] mean and std.dev: (397.78, 137.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1048) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (643, 1784, 4418) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11968) [M::mem_pestat] mean and std.dev: (2772.20, 2645.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15743) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (617, 1575, 4135) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11171) [M::mem_pestat] mean and std.dev: (2715.32, 2665.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14689) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 261.865 CPU sec, 16.727 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3414, 91793, 3239, 3451) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (602, 1681, 4060) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10976) [M::mem_pestat] mean and std.dev: (2679.71, 2585.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14434) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 381, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864) [M::mem_pestat] mean and std.dev: (397.92, 138.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1051) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (638, 1774, 4365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11819) [M::mem_pestat] mean and std.dev: (2770.25, 2649.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15546) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (624, 1598, 4149) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11199) [M::mem_pestat] mean and std.dev: (2683.06, 2588.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14724) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 261.325 CPU sec, 16.830 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3375, 91965, 3318, 3400) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (653, 1679, 4235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11399) [M::mem_pestat] mean and std.dev: (2746.02, 2643.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14981) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 381, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864) [M::mem_pestat] mean and std.dev: (397.39, 138.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1051) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (615, 1718, 4167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11271) [M::mem_pestat] mean and std.dev: (2748.56, 2657.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14823) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (629, 1625, 3997) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10733) [M::mem_pestat] mean and std.dev: (2655.65, 2588.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14101) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 260.878 CPU sec, 16.538 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3536, 92139, 3345, 3499) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (634, 1616, 4143) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11161) [M::mem_pestat] mean and std.dev: (2720.18, 2645.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14670) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (304, 380, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 862) [M::mem_pestat] mean and std.dev: (397.59, 138.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1048) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (603, 1656, 4105) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11109) [M::mem_pestat] mean and std.dev: (2675.63, 2619.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14611) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (660, 1694, 4268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11484) [M::mem_pestat] mean and std.dev: (2718.16, 2571.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15092) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 261.265 CPU sec, 16.801 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3421, 91657, 3236, 3412) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (647, 1798, 4493) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12185) [M::mem_pestat] mean and std.dev: (2849.16, 2681.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16031) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 381, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 867) [M::mem_pestat] mean and std.dev: (397.88, 138.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1055) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (602, 1596, 4139) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11213) [M::mem_pestat] mean and std.dev: (2656.14, 2628.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14750) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (677, 1626, 4170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11156) [M::mem_pestat] mean and std.dev: (2732.14, 2652.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14649) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 260.314 CPU sec, 16.501 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3437, 92373, 3255, 3460) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (652, 1791, 4270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11506) [M::mem_pestat] mean and std.dev: (2772.77, 2594.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15124) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (304, 382, 493) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 871) [M::mem_pestat] mean and std.dev: (400.02, 139.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1060) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (606, 1497, 3843) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10317) [M::mem_pestat] mean and std.dev: (2570.33, 2551.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13554) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (648, 1572, 3996) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10692) [M::mem_pestat] mean and std.dev: (2662.51, 2599.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14040) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 264.182 CPU sec, 16.788 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3545, 91753, 3522, 3563) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (645, 1632, 4255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11475) [M::mem_pestat] mean and std.dev: (2714.02, 2613.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15085) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 380, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861) [M::mem_pestat] mean and std.dev: (397.25, 138.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1047) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (619, 1596, 4263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11551) [M::mem_pestat] mean and std.dev: (2723.88, 2673.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15195) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (621, 1546, 3706) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9876) [M::mem_pestat] mean and std.dev: (2537.00, 2510.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12961) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 260.658 CPU sec, 16.711 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3389, 92244, 3299, 3467) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (653, 1762, 4142) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11120) [M::mem_pestat] mean and std.dev: (2730.74, 2593.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14609) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 380, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864) [M::mem_pestat] mean and std.dev: (397.27, 137.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1051) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (592, 1602, 4245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11551) [M::mem_pestat] mean and std.dev: (2705.57, 2650.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15204) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (648, 1633, 4116) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11052) [M::mem_pestat] mean and std.dev: (2673.78, 2566.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14520) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 261.236 CPU sec, 16.596 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3523, 92322, 3406, 3433) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (653, 1672, 4045) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10829) [M::mem_pestat] mean and std.dev: (2709.51, 2609.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14221) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 381, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861) [M::mem_pestat] mean and std.dev: (397.36, 137.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1047) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (629, 1689, 4215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11387) [M::mem_pestat] mean and std.dev: (2741.25, 2667.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14973) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (654, 1698, 4195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11277) [M::mem_pestat] mean and std.dev: (2752.75, 2638.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14818) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 262.827 CPU sec, 16.843 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3503, 92043, 3363, 3347) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (649, 1718, 4020) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10762) [M::mem_pestat] mean and std.dev: (2712.77, 2602.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14133) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 380, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864) [M::mem_pestat] mean and std.dev: (396.89, 137.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1051) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (634, 1645, 4096) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11020) [M::mem_pestat] mean and std.dev: (2685.02, 2586.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14482) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (654, 1667, 4086) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10950) [M::mem_pestat] mean and std.dev: (2702.52, 2582.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14382) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 260.964 CPU sec, 16.601 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3454, 92672, 3300, 3403) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (644, 1634, 4138) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11126) [M::mem_pestat] mean and std.dev: (2681.49, 2582.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14620) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 382, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 867) [M::mem_pestat] mean and std.dev: (398.84, 139.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1055) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (613, 1595, 4206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11392) [M::mem_pestat] mean and std.dev: (2714.96, 2673.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14985) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (639, 1578, 4054) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10884) [M::mem_pestat] mean and std.dev: (2648.80, 2581.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14299) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 260.027 CPU sec, 16.631 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3317, 91914, 3322, 3409) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (661, 1680, 4466) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12076) [M::mem_pestat] mean and std.dev: (2826.78, 2690.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15881) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 382, 492) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 870) [M::mem_pestat] mean and std.dev: (399.07, 138.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1059) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (605, 1741, 4276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11618) [M::mem_pestat] mean and std.dev: (2749.98, 2646.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15289) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (625, 1641, 4061) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10933) [M::mem_pestat] mean and std.dev: (2685.10, 2619.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14369) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 261.334 CPU sec, 16.642 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3293, 91696, 3402, 3491) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (610, 1574, 4187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11341) [M::mem_pestat] mean and std.dev: (2679.50, 2603.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14918) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (304, 382, 492) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 868) [M::mem_pestat] mean and std.dev: (399.05, 138.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1056) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (622, 1620, 4314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11698) [M::mem_pestat] mean and std.dev: (2719.84, 2644.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15390) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (651, 1625, 4190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11268) [M::mem_pestat] mean and std.dev: (2677.51, 2565.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14807) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 263.177 CPU sec, 16.963 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3352, 91906, 3266, 3435) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (662, 1610, 4033) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10775) [M::mem_pestat] mean and std.dev: (2662.04, 2532.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14146) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 380, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864) [M::mem_pestat] mean and std.dev: (397.08, 137.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1051) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (620, 1664, 4192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11336) [M::mem_pestat] mean and std.dev: (2704.02, 2638.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14908) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (616, 1663, 4120) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11128) [M::mem_pestat] mean and std.dev: (2703.82, 2643.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14632) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 262.656 CPU sec, 16.818 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3397, 92608, 3225, 3454) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (650, 1726, 4369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11807) [M::mem_pestat] mean and std.dev: (2784.95, 2655.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15526) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 379, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 863) [M::mem_pestat] mean and std.dev: (396.60, 137.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1050) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (639, 1732, 4258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11496) [M::mem_pestat] mean and std.dev: (2755.18, 2621.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15115) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (653, 1685, 4181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11237) [M::mem_pestat] mean and std.dev: (2716.31, 2602.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14765) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 267.908 CPU sec, 17.208 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3339, 92403, 3311, 3424) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (610, 1548, 4172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11296) [M::mem_pestat] mean and std.dev: (2661.00, 2604.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14858) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 380, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864) [M::mem_pestat] mean and std.dev: (397.16, 138.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1051) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (637, 1696, 4173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11245) [M::mem_pestat] mean and std.dev: (2721.68, 2623.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14781) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (664, 1807, 4259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11449) [M::mem_pestat] mean and std.dev: (2762.14, 2585.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15044) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 262.206 CPU sec, 16.666 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3547, 91625, 3262, 3374) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (656, 1696, 4094) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10970) [M::mem_pestat] mean and std.dev: (2689.78, 2558.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14408) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 381, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 869) [M::mem_pestat] mean and std.dev: (397.97, 139.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1058) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (628, 1662, 4033) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10843) [M::mem_pestat] mean and std.dev: (2668.29, 2584.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14248) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (631, 1592, 4013) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10777) [M::mem_pestat] mean and std.dev: (2620.23, 2528.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14159) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 262.673 CPU sec, 16.681 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3480, 92438, 3303, 3470) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (680, 1688, 4123) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11009) [M::mem_pestat] mean and std.dev: (2726.29, 2605.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14452) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 381, 492) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 870) [M::mem_pestat] mean and std.dev: (398.68, 139.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1059) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (624, 1625, 4108) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11076) [M::mem_pestat] mean and std.dev: (2676.33, 2600.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14560) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (650, 1653, 4024) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10772) [M::mem_pestat] mean and std.dev: (2654.65, 2537.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14146) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 260.603 CPU sec, 16.707 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3532, 92146, 3232, 3476) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (657, 1659, 4190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11256) [M::mem_pestat] mean and std.dev: (2705.26, 2605.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14789) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 381, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861) [M::mem_pestat] mean and std.dev: (397.38, 137.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1047) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (626, 1736, 3968) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10652) [M::mem_pestat] mean and std.dev: (2675.77, 2562.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13994) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (626, 1539, 4244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11480) [M::mem_pestat] mean and std.dev: (2695.21, 2652.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15098) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 263.644 CPU sec, 16.746 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3475, 91835, 3221, 3514) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (622, 1584, 4032) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10852) [M::mem_pestat] mean and std.dev: (2650.28, 2568.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14262) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 381, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864) [M::mem_pestat] mean and std.dev: (397.77, 138.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1051) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (644, 1753, 4309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11639) [M::mem_pestat] mean and std.dev: (2769.23, 2650.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15304) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (674, 1697, 4181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11195) [M::mem_pestat] mean and std.dev: (2708.68, 2561.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14702) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 263.372 CPU sec, 16.880 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3284, 92491, 3208, 3435) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (650, 1649, 4121) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11063) [M::mem_pestat] mean and std.dev: (2706.84, 2605.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14534) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 381, 492) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 870) [M::mem_pestat] mean and std.dev: (398.10, 138.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1059) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (669, 1818, 4516) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12210) [M::mem_pestat] mean and std.dev: (2887.09, 2709.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16057) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (656, 1655, 4053) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10847) [M::mem_pestat] mean and std.dev: (2681.13, 2581.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14244) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 263.657 CPU sec, 16.723 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3417, 92311, 3265, 3340) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (618, 1625, 4015) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10809) [M::mem_pestat] mean and std.dev: (2685.95, 2617.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14206) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 381, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 858) [M::mem_pestat] mean and std.dev: (397.04, 137.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1043) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (655, 1809, 4245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11425) [M::mem_pestat] mean and std.dev: (2786.69, 2652.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15015) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (641, 1700, 4264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11510) [M::mem_pestat] mean and std.dev: (2774.15, 2664.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15133) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 262.155 CPU sec, 16.670 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3497, 92127, 3218, 3527) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (649, 1699, 4156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11170) [M::mem_pestat] mean and std.dev: (2727.95, 2622.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14677) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 381, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 866) [M::mem_pestat] mean and std.dev: (398.04, 138.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1054) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (598, 1536, 3884) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10456) [M::mem_pestat] mean and std.dev: (2601.93, 2603.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13742) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (650, 1717, 4360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11780) [M::mem_pestat] mean and std.dev: (2782.73, 2662.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15490) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 263.947 CPU sec, 16.968 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3314, 92205, 3242, 3471) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (645, 1593, 4085) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10965) [M::mem_pestat] mean and std.dev: (2668.46, 2608.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14405) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (301, 379, 486) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 856) [M::mem_pestat] mean and std.dev: (394.87, 136.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1041) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (574, 1630, 4102) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11158) [M::mem_pestat] mean and std.dev: (2636.00, 2596.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14686) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (646, 1578, 4046) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10846) [M::mem_pestat] mean and std.dev: (2651.97, 2573.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14246) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 262.710 CPU sec, 16.658 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3493, 92253, 3383, 3436) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (674, 1672, 4237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11363) [M::mem_pestat] mean and std.dev: (2766.70, 2653.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14926) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 380, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864) [M::mem_pestat] mean and std.dev: (397.43, 138.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1051) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (650, 1717, 4316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11648) [M::mem_pestat] mean and std.dev: (2753.79, 2641.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15314) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (599, 1574, 4060) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10982) [M::mem_pestat] mean and std.dev: (2625.33, 2581.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14443) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 265.342 CPU sec, 17.013 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3515, 91981, 3248, 3522) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (659, 1628, 4101) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10985) [M::mem_pestat] mean and std.dev: (2725.82, 2640.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14427) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 381, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 867) [M::mem_pestat] mean and std.dev: (398.05, 138.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1055) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (590, 1637, 4089) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11087) [M::mem_pestat] mean and std.dev: (2660.12, 2603.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14586) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (640, 1640, 3948) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10564) [M::mem_pestat] mean and std.dev: (2636.28, 2537.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13872) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 259.562 CPU sec, 16.509 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3547, 93022, 3406, 3502) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (639, 1605, 4109) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11049) [M::mem_pestat] mean and std.dev: (2704.17, 2636.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14519) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 379, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860) [M::mem_pestat] mean and std.dev: (395.71, 137.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1046) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (612, 1635, 4015) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10821) [M::mem_pestat] mean and std.dev: (2657.92, 2608.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14224) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (625, 1590, 4198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11344) [M::mem_pestat] mean and std.dev: (2726.12, 2676.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14917) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 262.438 CPU sec, 16.691 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3360, 92514, 3285, 3433) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (696, 1709, 4155) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11073) [M::mem_pestat] mean and std.dev: (2731.71, 2592.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14532) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 380, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 866) [M::mem_pestat] mean and std.dev: (397.35, 139.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1054) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (618, 1596, 3928) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10548) [M::mem_pestat] mean and std.dev: (2616.84, 2571.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13858) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (677, 1784, 4293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11525) [M::mem_pestat] mean and std.dev: (2775.39, 2597.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15141) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 263.049 CPU sec, 16.709 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3484, 92069, 3277, 3360) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (675, 1737, 4365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11745) [M::mem_pestat] mean and std.dev: (2834.13, 2710.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15435) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 380, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 863) [M::mem_pestat] mean and std.dev: (396.74, 137.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1050) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (643, 1769, 4151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11167) [M::mem_pestat] mean and std.dev: (2729.74, 2575.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14675) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (657, 1744, 4084) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10938) [M::mem_pestat] mean and std.dev: (2698.91, 2557.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14365) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 261.240 CPU sec, 16.997 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3428, 92027, 3516, 3492) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (643, 1633, 4100) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11014) [M::mem_pestat] mean and std.dev: (2673.05, 2583.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14471) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 381, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 866) [M::mem_pestat] mean and std.dev: (397.58, 138.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1054) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (604, 1638, 4096) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11080) [M::mem_pestat] mean and std.dev: (2691.88, 2640.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14572) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (654, 1801, 4348) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11736) [M::mem_pestat] mean and std.dev: (2792.95, 2640.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15430) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 264.005 CPU sec, 16.792 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3315, 92399, 3313, 3437) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (652, 1780, 4322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11662) [M::mem_pestat] mean and std.dev: (2811.89, 2650.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15332) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (301, 379, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 862) [M::mem_pestat] mean and std.dev: (396.22, 138.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1049) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (613, 1668, 4084) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11026) [M::mem_pestat] mean and std.dev: (2681.42, 2608.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14497) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (640, 1648, 4054) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10882) [M::mem_pestat] mean and std.dev: (2660.94, 2559.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14296) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 264.075 CPU sec, 16.885 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3289, 91960, 3316, 3444) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (670, 1767, 4169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11167) [M::mem_pestat] mean and std.dev: (2733.45, 2548.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14666) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 379, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860) [M::mem_pestat] mean and std.dev: (396.08, 137.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1046) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (611, 1578, 4051) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10931) [M::mem_pestat] mean and std.dev: (2631.35, 2587.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14371) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (662, 1608, 4130) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11066) [M::mem_pestat] mean and std.dev: (2698.40, 2593.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14534) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 262.483 CPU sec, 16.687 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3441, 91855, 3272, 3445) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (624, 1613, 4078) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10986) [M::mem_pestat] mean and std.dev: (2661.64, 2587.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14440) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 380, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 863) [M::mem_pestat] mean and std.dev: (396.94, 138.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1050) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (623, 1701, 4289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11621) [M::mem_pestat] mean and std.dev: (2748.69, 2640.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15287) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (680, 1678, 3991) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10613) [M::mem_pestat] mean and std.dev: (2688.30, 2585.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13924) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 263.913 CPU sec, 16.965 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3547, 91951, 3184, 3276) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (643, 1666, 4163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11203) [M::mem_pestat] mean and std.dev: (2743.57, 2649.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14723) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 380, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860) [M::mem_pestat] mean and std.dev: (396.41, 137.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1046) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (632, 1801, 4375) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11861) [M::mem_pestat] mean and std.dev: (2782.86, 2635.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15604) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (658, 1718, 4107) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11005) [M::mem_pestat] mean and std.dev: (2727.04, 2598.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14454) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 264.684 CPU sec, 16.807 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3415, 92059, 3414, 3411) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (637, 1661, 4030) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10816) [M::mem_pestat] mean and std.dev: (2671.17, 2537.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14209) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 379, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 866) [M::mem_pestat] mean and std.dev: (397.48, 139.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1054) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (601, 1640, 4171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11311) [M::mem_pestat] mean and std.dev: (2707.69, 2642.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14881) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (676, 1808, 4281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11491) [M::mem_pestat] mean and std.dev: (2790.79, 2594.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15096) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 266.232 CPU sec, 17.013 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3366, 92177, 3278, 3516) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (671, 1586, 4042) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10784) [M::mem_pestat] mean and std.dev: (2675.71, 2614.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14155) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (301, 378, 487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 859) [M::mem_pestat] mean and std.dev: (395.04, 137.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1045) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (624, 1706, 4125) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11127) [M::mem_pestat] mean and std.dev: (2711.77, 2635.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14628) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (658, 1668, 4051) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10837) [M::mem_pestat] mean and std.dev: (2683.70, 2582.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14230) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 263.906 CPU sec, 16.797 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3435, 92450, 3216, 3431) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (630, 1581, 4030) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10830) [M::mem_pestat] mean and std.dev: (2684.20, 2610.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14230) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 379, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861) [M::mem_pestat] mean and std.dev: (396.53, 137.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1047) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (622, 1669, 3997) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10747) [M::mem_pestat] mean and std.dev: (2649.66, 2563.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14122) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (642, 1599, 4191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11289) [M::mem_pestat] mean and std.dev: (2702.63, 2618.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14838) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 263.109 CPU sec, 16.754 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3501, 92510, 3267, 3396) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (639, 1644, 4030) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10812) [M::mem_pestat] mean and std.dev: (2664.80, 2571.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14203) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 379, 487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 857) [M::mem_pestat] mean and std.dev: (396.01, 137.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1042) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (635, 1666, 4372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11846) [M::mem_pestat] mean and std.dev: (2794.78, 2718.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15583) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (624, 1552, 3881) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10395) [M::mem_pestat] mean and std.dev: (2624.29, 2586.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13652) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 265.429 CPU sec, 17.091 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3553, 92800, 3364, 3528) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (616, 1612, 4105) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11083) [M::mem_pestat] mean and std.dev: (2708.36, 2645.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14572) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (301, 379, 487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 859) [M::mem_pestat] mean and std.dev: (395.78, 137.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1045) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (635, 1745, 4231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11423) [M::mem_pestat] mean and std.dev: (2735.92, 2627.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15019) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (637, 1614, 4126) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11104) [M::mem_pestat] mean and std.dev: (2657.33, 2574.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14593) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 265.656 CPU sec, 16.842 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3369, 92042, 3238, 3470) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (651, 1657, 4143) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11127) [M::mem_pestat] mean and std.dev: (2721.53, 2619.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14619) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 379, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860) [M::mem_pestat] mean and std.dev: (395.92, 137.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1046) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (572, 1632, 4131) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11249) [M::mem_pestat] mean and std.dev: (2649.81, 2597.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14808) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (630, 1736, 4136) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11148) [M::mem_pestat] mean and std.dev: (2727.52, 2628.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14654) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 267.910 CPU sec, 17.211 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3507, 92615, 3320, 3419) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (652, 1565, 4015) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10741) [M::mem_pestat] mean and std.dev: (2649.05, 2592.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14104) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 380, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860) [M::mem_pestat] mean and std.dev: (396.78, 138.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1046) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (628, 1735, 4135) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11149) [M::mem_pestat] mean and std.dev: (2716.02, 2623.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14656) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (605, 1557, 3899) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10487) [M::mem_pestat] mean and std.dev: (2592.79, 2534.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13781) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 266.333 CPU sec, 16.893 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3424, 92547, 3289, 3388) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (637, 1627, 4066) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10924) [M::mem_pestat] mean and std.dev: (2668.86, 2601.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14353) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (301, 378, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 862) [M::mem_pestat] mean and std.dev: (395.06, 137.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1049) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (673, 1812, 4276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11482) [M::mem_pestat] mean and std.dev: (2797.61, 2680.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15085) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (657, 1631, 4055) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10851) [M::mem_pestat] mean and std.dev: (2668.19, 2566.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14249) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 262.713 CPU sec, 16.697 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3384, 92220, 3339, 3353) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (618, 1591, 4352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11820) [M::mem_pestat] mean and std.dev: (2736.02, 2648.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15554) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (301, 378, 487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 859) [M::mem_pestat] mean and std.dev: (395.37, 137.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1045) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (641, 1701, 4277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11549) [M::mem_pestat] mean and std.dev: (2753.36, 2649.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15185) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (638, 1541, 4021) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10787) [M::mem_pestat] mean and std.dev: (2644.49, 2577.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14170) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 265.388 CPU sec, 16.881 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3490, 91548, 3264, 3273) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (668, 1630, 4116) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11012) [M::mem_pestat] mean and std.dev: (2705.40, 2599.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14460) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 379, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 863) [M::mem_pestat] mean and std.dev: (396.43, 138.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1050) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (597, 1618, 4192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11382) [M::mem_pestat] mean and std.dev: (2705.97, 2663.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14977) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (631, 1584, 4190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11308) [M::mem_pestat] mean and std.dev: (2661.96, 2565.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14867) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 269.797 CPU sec, 17.145 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3345, 92661, 3313, 3536) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (644, 1651, 4093) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10991) [M::mem_pestat] mean and std.dev: (2685.04, 2616.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14440) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 379, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 863) [M::mem_pestat] mean and std.dev: (396.40, 138.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1050) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (629, 1747, 4147) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11183) [M::mem_pestat] mean and std.dev: (2707.76, 2581.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14701) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (623, 1603, 3959) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10631) [M::mem_pestat] mean and std.dev: (2639.81, 2567.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13967) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 273.162 CPU sec, 17.347 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3464, 92115, 3288, 3471) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (677, 1683, 4133) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11045) [M::mem_pestat] mean and std.dev: (2711.93, 2604.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14501) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 379, 487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 857) [M::mem_pestat] mean and std.dev: (395.72, 137.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1042) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (593, 1656, 4207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11435) [M::mem_pestat] mean and std.dev: (2700.74, 2641.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15049) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (644, 1586, 3932) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10508) [M::mem_pestat] mean and std.dev: (2623.95, 2535.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13796) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 273.668 CPU sec, 17.980 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3387, 92395, 3322, 3423) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (649, 1594, 4035) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10807) [M::mem_pestat] mean and std.dev: (2689.45, 2627.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14193) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (300, 378, 487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861) [M::mem_pestat] mean and std.dev: (394.72, 137.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1048) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (596, 1605, 4200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11408) [M::mem_pestat] mean and std.dev: (2693.18, 2641.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15012) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (664, 1666, 3991) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10645) [M::mem_pestat] mean and std.dev: (2675.92, 2546.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13972) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 270.255 CPU sec, 17.310 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3411, 92108, 3274, 3356) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (673, 1658, 4137) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11065) [M::mem_pestat] mean and std.dev: (2694.24, 2559.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14529) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (301, 378, 487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 859) [M::mem_pestat] mean and std.dev: (394.85, 137.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1045) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (623, 1695, 4038) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10868) [M::mem_pestat] mean and std.dev: (2686.27, 2604.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14283) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (635, 1631, 3976) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10658) [M::mem_pestat] mean and std.dev: (2669.31, 2612.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13999) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 270.967 CPU sec, 17.287 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3463, 92943, 3348, 3413) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (637, 1638, 4330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11716) [M::mem_pestat] mean and std.dev: (2738.32, 2658.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15409) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (299, 377, 485) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 857) [M::mem_pestat] mean and std.dev: (393.07, 136.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1043) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (651, 1701, 4060) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10878) [M::mem_pestat] mean and std.dev: (2728.11, 2653.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14287) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (671, 1671, 4151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11111) [M::mem_pestat] mean and std.dev: (2734.70, 2632.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14591) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 266.355 CPU sec, 17.386 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3406, 93073, 3329, 3353) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (664, 1631, 3995) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10657) [M::mem_pestat] mean and std.dev: (2664.41, 2580.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13988) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (300, 379, 485) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 855) [M::mem_pestat] mean and std.dev: (394.07, 136.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1040) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (645, 1635, 4152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11166) [M::mem_pestat] mean and std.dev: (2711.15, 2654.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14673) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (625, 1569, 4067) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10951) [M::mem_pestat] mean and std.dev: (2616.51, 2530.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14393) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 261.637 CPU sec, 16.577 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3459, 92926, 3218, 3384) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (637, 1631, 4099) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11023) [M::mem_pestat] mean and std.dev: (2678.08, 2584.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14485) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (301, 378, 485) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 853) [M::mem_pestat] mean and std.dev: (393.61, 135.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1037) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (590, 1683, 4070) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11030) [M::mem_pestat] mean and std.dev: (2684.29, 2635.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14510) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (665, 1683, 4270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11480) [M::mem_pestat] mean and std.dev: (2757.34, 2643.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15085) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 264.805 CPU sec, 17.027 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3383, 92770, 3266, 3401) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (697, 1672, 4250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11356) [M::mem_pestat] mean and std.dev: (2760.23, 2618.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14909) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 379, 486) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 854) [M::mem_pestat] mean and std.dev: (395.11, 136.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1038) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (629, 1691, 4128) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11126) [M::mem_pestat] mean and std.dev: (2690.59, 2600.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14625) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (692, 1719, 4289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11483) [M::mem_pestat] mean and std.dev: (2783.42, 2636.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15080) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 259.511 CPU sec, 16.452 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3629, 93253, 3373, 3376) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (648, 1678, 3973) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10623) [M::mem_pestat] mean and std.dev: (2666.04, 2587.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13948) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 379, 487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 857) [M::mem_pestat] mean and std.dev: (395.87, 136.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1042) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (647, 1830, 4417) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11957) [M::mem_pestat] mean and std.dev: (2825.05, 2679.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15727) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (682, 1643, 4041) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10759) [M::mem_pestat] mean and std.dev: (2673.24, 2578.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14118) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 261.333 CPU sec, 16.691 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3428, 92848, 3445, 3503) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (617, 1513, 4151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11219) [M::mem_pestat] mean and std.dev: (2685.70, 2652.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14753) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 379, 486) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 852) [M::mem_pestat] mean and std.dev: (395.02, 136.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1035) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (587, 1625, 4257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11597) [M::mem_pestat] mean and std.dev: (2735.81, 2682.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15267) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (612, 1617, 3933) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10575) [M::mem_pestat] mean and std.dev: (2651.65, 2579.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13896) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 262.483 CPU sec, 16.694 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3445, 93015, 3367, 3553) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (642, 1709, 4178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11250) [M::mem_pestat] mean and std.dev: (2718.31, 2609.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14786) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 380, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 863) [M::mem_pestat] mean and std.dev: (396.45, 137.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1050) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (599, 1615, 4292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11678) [M::mem_pestat] mean and std.dev: (2715.86, 2678.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15371) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (640, 1693, 4003) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10729) [M::mem_pestat] mean and std.dev: (2690.08, 2599.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14092) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 264.240 CPU sec, 16.830 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3453, 92371, 3236, 3463) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (664, 1655, 3976) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10600) [M::mem_pestat] mean and std.dev: (2678.55, 2616.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13912) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 379, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 858) [M::mem_pestat] mean and std.dev: (395.99, 137.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1043) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (650, 1716, 4022) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10766) [M::mem_pestat] mean and std.dev: (2653.90, 2543.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14138) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (633, 1682, 4237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11445) [M::mem_pestat] mean and std.dev: (2741.22, 2613.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15049) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 262.595 CPU sec, 16.725 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3390, 91892, 3146, 3276) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (639, 1572, 3983) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10671) [M::mem_pestat] mean and std.dev: (2628.98, 2564.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14015) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 379, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860) [M::mem_pestat] mean and std.dev: (396.22, 137.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1046) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (654, 1732, 4344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11724) [M::mem_pestat] mean and std.dev: (2814.02, 2717.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15414) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (667, 1737, 4247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11407) [M::mem_pestat] mean and std.dev: (2723.19, 2548.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14987) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 263.402 CPU sec, 16.909 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3471, 92893, 3256, 3463) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (641, 1602, 4076) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10946) [M::mem_pestat] mean and std.dev: (2657.10, 2581.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14381) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 380, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864) [M::mem_pestat] mean and std.dev: (396.93, 137.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1051) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (614, 1678, 4459) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12149) [M::mem_pestat] mean and std.dev: (2815.32, 2733.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15994) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (639, 1560, 3953) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10581) [M::mem_pestat] mean and std.dev: (2622.84, 2580.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13895) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 262.533 CPU sec, 16.786 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3435, 92230, 3277, 3355) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (644, 1703, 4340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11732) [M::mem_pestat] mean and std.dev: (2751.57, 2597.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15428) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 380, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 858) [M::mem_pestat] mean and std.dev: (396.53, 136.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1043) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (603, 1630, 4176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11322) [M::mem_pestat] mean and std.dev: (2709.58, 2633.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14895) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (644, 1611, 3941) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10535) [M::mem_pestat] mean and std.dev: (2656.89, 2600.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13832) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 261.049 CPU sec, 16.714 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3585, 92585, 3387, 3499) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (659, 1631, 4031) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10775) [M::mem_pestat] mean and std.dev: (2679.23, 2568.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14147) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 381, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861) [M::mem_pestat] mean and std.dev: (397.22, 138.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1047) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (631, 1840, 4327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11719) [M::mem_pestat] mean and std.dev: (2813.63, 2655.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15415) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (678, 1691, 4054) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10806) [M::mem_pestat] mean and std.dev: (2757.72, 2658.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14182) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 261.473 CPU sec, 16.591 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3481, 91639, 3309, 3565) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (672, 1659, 4173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11175) [M::mem_pestat] mean and std.dev: (2746.83, 2651.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14676) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 380, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 863) [M::mem_pestat] mean and std.dev: (396.69, 137.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1050) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (627, 1731, 4379) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11883) [M::mem_pestat] mean and std.dev: (2754.56, 2655.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15635) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (643, 1722, 4079) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10951) [M::mem_pestat] mean and std.dev: (2704.93, 2589.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14387) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 261.836 CPU sec, 16.871 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3450, 92535, 3213, 3463) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (642, 1679, 4076) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10944) [M::mem_pestat] mean and std.dev: (2667.16, 2550.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14378) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 381, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864) [M::mem_pestat] mean and std.dev: (398.03, 138.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1051) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (590, 1691, 4020) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10880) [M::mem_pestat] mean and std.dev: (2677.74, 2606.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14310) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (603, 1596, 4137) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11205) [M::mem_pestat] mean and std.dev: (2684.14, 2638.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14739) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 260.283 CPU sec, 16.543 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3478, 91885, 3284, 3428) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (628, 1605, 4032) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10840) [M::mem_pestat] mean and std.dev: (2676.20, 2628.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14244) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 381, 492) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 870) [M::mem_pestat] mean and std.dev: (398.54, 139.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1059) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (628, 1757, 4447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12085) [M::mem_pestat] mean and std.dev: (2825.09, 2707.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15904) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (663, 1743, 4347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11715) [M::mem_pestat] mean and std.dev: (2816.59, 2685.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15399) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 263.988 CPU sec, 16.936 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3474, 92731, 3352, 3308) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (695, 1886, 4241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11333) [M::mem_pestat] mean and std.dev: (2820.91, 2648.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14879) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 379, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864) [M::mem_pestat] mean and std.dev: (396.53, 137.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1051) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (619, 1690, 4290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11632) [M::mem_pestat] mean and std.dev: (2742.71, 2644.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15303) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (623, 1576, 4057) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10925) [M::mem_pestat] mean and std.dev: (2684.17, 2626.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14359) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 260.472 CPU sec, 16.533 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3466, 91900, 3327, 3421) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (646, 1680, 4266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11506) [M::mem_pestat] mean and std.dev: (2746.81, 2624.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15126) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 380, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861) [M::mem_pestat] mean and std.dev: (397.14, 137.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1047) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (615, 1655, 4087) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11031) [M::mem_pestat] mean and std.dev: (2678.91, 2606.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14503) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (667, 1577, 3941) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10489) [M::mem_pestat] mean and std.dev: (2645.50, 2544.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13763) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 262.347 CPU sec, 16.700 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3404, 92012, 3315, 3316) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (674, 1701, 4135) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11057) [M::mem_pestat] mean and std.dev: (2731.25, 2603.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14518) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 380, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864) [M::mem_pestat] mean and std.dev: (397.48, 138.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1051) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (586, 1556, 4125) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11203) [M::mem_pestat] mean and std.dev: (2668.25, 2677.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14742) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (606, 1568, 4149) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11235) [M::mem_pestat] mean and std.dev: (2680.18, 2629.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14778) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 261.071 CPU sec, 16.684 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3366, 92121, 3235, 3418) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (610, 1433, 3765) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10075) [M::mem_pestat] mean and std.dev: (2509.82, 2536.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13230) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 380, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 867) [M::mem_pestat] mean and std.dev: (398.03, 138.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1055) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (627, 1642, 4070) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10956) [M::mem_pestat] mean and std.dev: (2614.97, 2551.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14399) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (648, 1536, 3884) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10356) [M::mem_pestat] mean and std.dev: (2578.99, 2518.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13592) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 262.720 CPU sec, 16.652 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3473, 91840, 3297, 3531) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (658, 1683, 4180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11224) [M::mem_pestat] mean and std.dev: (2750.38, 2626.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14746) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (305, 382, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 863) [M::mem_pestat] mean and std.dev: (398.33, 137.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1049) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (635, 1647, 4079) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10967) [M::mem_pestat] mean and std.dev: (2690.32, 2630.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14411) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (650, 1674, 4216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11348) [M::mem_pestat] mean and std.dev: (2717.62, 2607.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14914) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 264.800 CPU sec, 16.962 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3444, 93004, 3386, 3416) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (668, 1646, 4242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11390) [M::mem_pestat] mean and std.dev: (2708.17, 2610.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14964) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 381, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864) [M::mem_pestat] mean and std.dev: (397.59, 138.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1051) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (649, 1670, 4095) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10987) [M::mem_pestat] mean and std.dev: (2657.74, 2556.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14433) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (658, 1686, 4168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11188) [M::mem_pestat] mean and std.dev: (2693.59, 2562.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14698) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 261.558 CPU sec, 16.690 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3351, 92340, 3422, 3519) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (679, 1659, 3924) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10414) [M::mem_pestat] mean and std.dev: (2638.28, 2541.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13659) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (304, 381, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 862) [M::mem_pestat] mean and std.dev: (398.43, 137.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1048) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (663, 1750, 4304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11586) [M::mem_pestat] mean and std.dev: (2782.31, 2666.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15227) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (639, 1682, 4006) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10740) [M::mem_pestat] mean and std.dev: (2669.93, 2573.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14107) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 258.163 CPU sec, 16.410 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3564, 91816, 3259, 3442) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (636, 1660, 4068) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10932) [M::mem_pestat] mean and std.dev: (2659.48, 2567.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14364) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (304, 381, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 862) [M::mem_pestat] mean and std.dev: (398.19, 137.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1048) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (632, 1680, 4122) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11102) [M::mem_pestat] mean and std.dev: (2699.52, 2592.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14592) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (656, 1649, 4431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11981) [M::mem_pestat] mean and std.dev: (2773.19, 2672.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15756) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 259.034 CPU sec, 16.670 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3397, 91953, 3355, 3476) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (646, 1692, 4218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11362) [M::mem_pestat] mean and std.dev: (2699.88, 2578.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14934) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 382, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864) [M::mem_pestat] mean and std.dev: (398.05, 138.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1051) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (604, 1580, 4187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11353) [M::mem_pestat] mean and std.dev: (2705.91, 2671.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14936) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (624, 1639, 4244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11484) [M::mem_pestat] mean and std.dev: (2755.92, 2646.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15104) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 261.814 CPU sec, 16.600 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3481, 91908, 3219, 3467) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (666, 1663, 3959) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10545) [M::mem_pestat] mean and std.dev: (2653.95, 2545.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13838) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 380, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861) [M::mem_pestat] mean and std.dev: (397.25, 137.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1047) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (579, 1633, 4195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11427) [M::mem_pestat] mean and std.dev: (2710.16, 2685.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15043) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (659, 1689, 4034) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10784) [M::mem_pestat] mean and std.dev: (2693.38, 2573.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14159) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 262.106 CPU sec, 16.748 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3379, 91720, 3283, 3447) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (655, 1643, 4139) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11107) [M::mem_pestat] mean and std.dev: (2711.66, 2645.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14591) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (304, 380, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 865) [M::mem_pestat] mean and std.dev: (397.60, 137.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1052) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (611, 1666, 4168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11282) [M::mem_pestat] mean and std.dev: (2722.04, 2679.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14839) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (651, 1641, 3939) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10515) [M::mem_pestat] mean and std.dev: (2661.87, 2564.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13803) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 261.257 CPU sec, 16.552 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3534, 92492, 3335, 3410) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (601, 1557, 4168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11302) [M::mem_pestat] mean and std.dev: (2668.70, 2618.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14869) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 379, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860) [M::mem_pestat] mean and std.dev: (395.91, 136.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1046) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (633, 1566, 4270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11544) [M::mem_pestat] mean and std.dev: (2704.14, 2648.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15181) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (657, 1756, 4397) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11877) [M::mem_pestat] mean and std.dev: (2769.40, 2617.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15617) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 267.489 CPU sec, 17.015 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3459, 92371, 3332, 3411) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (618, 1661, 4031) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10857) [M::mem_pestat] mean and std.dev: (2699.02, 2611.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14270) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 380, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861) [M::mem_pestat] mean and std.dev: (396.91, 137.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1047) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (599, 1693, 4150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11252) [M::mem_pestat] mean and std.dev: (2722.51, 2654.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14803) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (660, 1667, 4181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11223) [M::mem_pestat] mean and std.dev: (2720.83, 2601.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14744) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 260.944 CPU sec, 16.739 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3464, 91795, 3343, 3434) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (625, 1611, 4017) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10801) [M::mem_pestat] mean and std.dev: (2629.85, 2549.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14193) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 380, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 867) [M::mem_pestat] mean and std.dev: (397.71, 138.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1055) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (640, 1678, 4185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11275) [M::mem_pestat] mean and std.dev: (2728.65, 2620.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14820) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (662, 1714, 4354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11738) [M::mem_pestat] mean and std.dev: (2791.19, 2657.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15430) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 262.683 CPU sec, 17.192 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3482, 92027, 3348, 3385) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (640, 1640, 3963) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10609) [M::mem_pestat] mean and std.dev: (2644.24, 2538.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13932) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (304, 381, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 859) [M::mem_pestat] mean and std.dev: (397.34, 137.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1044) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (645, 1659, 4241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11433) [M::mem_pestat] mean and std.dev: (2741.47, 2658.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15029) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (632, 1661, 4085) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10991) [M::mem_pestat] mean and std.dev: (2689.54, 2595.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14444) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 259.564 CPU sec, 16.555 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3433, 92298, 3304, 3396) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (643, 1613, 3980) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10654) [M::mem_pestat] mean and std.dev: (2674.46, 2617.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13991) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 381, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864) [M::mem_pestat] mean and std.dev: (397.31, 138.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1051) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (623, 1700, 4227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11435) [M::mem_pestat] mean and std.dev: (2721.95, 2612.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15039) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (591, 1608, 4103) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11127) [M::mem_pestat] mean and std.dev: (2656.29, 2580.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14639) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 260.666 CPU sec, 16.602 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3356, 91731, 3353, 3390) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (643, 1686, 3928) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10498) [M::mem_pestat] mean and std.dev: (2668.08, 2583.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13783) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 379, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 858) [M::mem_pestat] mean and std.dev: (396.56, 137.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1043) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (622, 1719, 4161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11239) [M::mem_pestat] mean and std.dev: (2727.46, 2660.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14778) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (670, 1730, 4172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11176) [M::mem_pestat] mean and std.dev: (2755.34, 2604.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14678) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 263.008 CPU sec, 16.764 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3406, 92441, 3114, 3472) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (647, 1616, 4075) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10931) [M::mem_pestat] mean and std.dev: (2670.16, 2585.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14359) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 380, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 863) [M::mem_pestat] mean and std.dev: (396.84, 138.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1050) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (630, 1634, 3968) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10644) [M::mem_pestat] mean and std.dev: (2661.92, 2592.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13982) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (645, 1649, 4047) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10851) [M::mem_pestat] mean and std.dev: (2626.12, 2514.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14253) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 261.963 CPU sec, 16.688 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3538, 92086, 3295, 3529) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (660, 1578, 3882) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10326) [M::mem_pestat] mean and std.dev: (2630.33, 2557.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13548) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 380, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861) [M::mem_pestat] mean and std.dev: (397.14, 137.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1047) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (654, 1709, 4159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11169) [M::mem_pestat] mean and std.dev: (2742.63, 2642.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14674) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (663, 1637, 3926) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10452) [M::mem_pestat] mean and std.dev: (2673.49, 2564.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13715) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 260.800 CPU sec, 16.602 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3352, 92180, 3377, 3313) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (669, 1706, 4123) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11031) [M::mem_pestat] mean and std.dev: (2707.80, 2586.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14485) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (304, 380, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 862) [M::mem_pestat] mean and std.dev: (397.40, 137.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1048) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (634, 1714, 4196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11320) [M::mem_pestat] mean and std.dev: (2727.10, 2644.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14882) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (630, 1594, 3941) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10563) [M::mem_pestat] mean and std.dev: (2607.17, 2541.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13874) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 261.789 CPU sec, 16.717 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3479, 92732, 3299, 3409) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (644, 1596, 3967) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10613) [M::mem_pestat] mean and std.dev: (2660.93, 2594.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13936) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (304, 381, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 865) [M::mem_pestat] mean and std.dev: (398.25, 137.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1052) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (609, 1590, 3818) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10236) [M::mem_pestat] mean and std.dev: (2601.60, 2603.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13445) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (623, 1651, 4127) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11135) [M::mem_pestat] mean and std.dev: (2699.87, 2614.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14639) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 261.621 CPU sec, 16.577 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3414, 92436, 3283, 3510) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (631, 1724, 4162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11224) [M::mem_pestat] mean and std.dev: (2747.55, 2649.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14755) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 380, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861) [M::mem_pestat] mean and std.dev: (396.72, 137.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1047) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (632, 1627, 4165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11231) [M::mem_pestat] mean and std.dev: (2696.36, 2632.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14764) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (659, 1610, 4000) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10682) [M::mem_pestat] mean and std.dev: (2665.10, 2582.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14023) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 262.438 CPU sec, 16.822 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3302, 92883, 3280, 3376) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (661, 1745, 4205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11293) [M::mem_pestat] mean and std.dev: (2744.34, 2596.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14837) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 380, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860) [M::mem_pestat] mean and std.dev: (396.44, 137.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1046) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (633, 1632, 4288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11598) [M::mem_pestat] mean and std.dev: (2718.42, 2650.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15253) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (668, 1713, 4189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11231) [M::mem_pestat] mean and std.dev: (2753.88, 2628.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14752) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 263.024 CPU sec, 16.719 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3466, 92086, 3244, 3399) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (661, 1529, 3939) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10495) [M::mem_pestat] mean and std.dev: (2620.40, 2564.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13773) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 379, 486) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 854) [M::mem_pestat] mean and std.dev: (395.54, 136.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1038) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (570, 1609, 4281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11703) [M::mem_pestat] mean and std.dev: (2693.81, 2651.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15414) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (611, 1583, 4128) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11162) [M::mem_pestat] mean and std.dev: (2651.83, 2596.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14679) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 263.849 CPU sec, 16.947 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3382, 92320, 3299, 3376) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (631, 1596, 4051) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10891) [M::mem_pestat] mean and std.dev: (2676.72, 2611.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14311) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 380, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864) [M::mem_pestat] mean and std.dev: (397.77, 138.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1051) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (612, 1671, 4162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11262) [M::mem_pestat] mean and std.dev: (2704.56, 2616.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14812) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (653, 1621, 3972) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10610) [M::mem_pestat] mean and std.dev: (2642.10, 2565.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13929) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 264.497 CPU sec, 16.785 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3549, 92152, 3269, 3354) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (650, 1629, 4243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11429) [M::mem_pestat] mean and std.dev: (2701.96, 2595.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15022) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 380, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 866) [M::mem_pestat] mean and std.dev: (397.45, 138.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1054) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (645, 1729, 4093) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10989) [M::mem_pestat] mean and std.dev: (2702.10, 2585.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14437) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (643, 1581, 3895) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10399) [M::mem_pestat] mean and std.dev: (2622.32, 2576.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13651) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 262.928 CPU sec, 16.739 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3533, 92830, 3241, 3431) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (656, 1649, 4200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11288) [M::mem_pestat] mean and std.dev: (2699.28, 2583.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14832) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 380, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864) [M::mem_pestat] mean and std.dev: (397.07, 138.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1051) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (582, 1658, 4084) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11088) [M::mem_pestat] mean and std.dev: (2698.52, 2664.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14590) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (682, 1659, 4022) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10702) [M::mem_pestat] mean and std.dev: (2675.02, 2560.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14042) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 264.255 CPU sec, 16.802 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3424, 92240, 3303, 3509) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (641, 1561, 3993) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10697) [M::mem_pestat] mean and std.dev: (2639.42, 2572.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14049) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 380, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 866) [M::mem_pestat] mean and std.dev: (397.20, 138.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1054) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (589, 1638, 4145) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11257) [M::mem_pestat] mean and std.dev: (2681.17, 2620.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14813) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (649, 1762, 4120) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11062) [M::mem_pestat] mean and std.dev: (2707.40, 2561.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14533) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 265.386 CPU sec, 17.051 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3443, 92588, 3331, 3435) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (669, 1680, 4096) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10950) [M::mem_pestat] mean and std.dev: (2743.55, 2651.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14377) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 381, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864) [M::mem_pestat] mean and std.dev: (397.50, 138.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1051) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (665, 1657, 4153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11129) [M::mem_pestat] mean and std.dev: (2698.90, 2611.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14617) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (640, 1603, 4136) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11128) [M::mem_pestat] mean and std.dev: (2679.53, 2581.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14624) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 263.914 CPU sec, 16.773 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3341, 91451, 3314, 3503) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (640, 1667, 3975) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10645) [M::mem_pestat] mean and std.dev: (2677.97, 2589.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13980) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 380, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860) [M::mem_pestat] mean and std.dev: (396.16, 137.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1046) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (629, 1724, 4190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11312) [M::mem_pestat] mean and std.dev: (2705.54, 2587.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14873) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (672, 1745, 4277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11487) [M::mem_pestat] mean and std.dev: (2771.92, 2651.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15092) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 266.535 CPU sec, 17.138 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3341, 92937, 3220, 3421) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (656, 1649, 3956) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10556) [M::mem_pestat] mean and std.dev: (2660.36, 2591.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13856) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 381, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 866) [M::mem_pestat] mean and std.dev: (397.53, 138.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1054) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (609, 1688, 4237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11493) [M::mem_pestat] mean and std.dev: (2715.20, 2628.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15121) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (607, 1605, 4039) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10903) [M::mem_pestat] mean and std.dev: (2638.01, 2566.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14335) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 264.698 CPU sec, 16.807 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3360, 92571, 3343, 3332) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (657, 1635, 4127) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11067) [M::mem_pestat] mean and std.dev: (2680.22, 2586.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14537) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 380, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860) [M::mem_pestat] mean and std.dev: (396.30, 137.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1046) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (651, 1700, 4016) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10746) [M::mem_pestat] mean and std.dev: (2702.94, 2608.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14111) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (658, 1666, 4109) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11011) [M::mem_pestat] mean and std.dev: (2666.68, 2556.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14462) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 265.555 CPU sec, 17.129 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3410, 91825, 3309, 3526) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (673, 1664, 4156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11122) [M::mem_pestat] mean and std.dev: (2720.71, 2594.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14605) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 380, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 866) [M::mem_pestat] mean and std.dev: (397.34, 138.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1054) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (614, 1658, 4219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11429) [M::mem_pestat] mean and std.dev: (2745.57, 2689.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15034) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (633, 1642, 4227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11415) [M::mem_pestat] mean and std.dev: (2757.09, 2672.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15009) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 267.318 CPU sec, 16.956 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3461, 92325, 3225, 3312) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (638, 1703, 4144) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11156) [M::mem_pestat] mean and std.dev: (2716.37, 2601.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14662) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 380, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 863) [M::mem_pestat] mean and std.dev: (396.75, 138.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1050) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (621, 1643, 4141) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11181) [M::mem_pestat] mean and std.dev: (2723.36, 2690.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14701) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (693, 1670, 4223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11283) [M::mem_pestat] mean and std.dev: (2720.97, 2563.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14813) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 264.078 CPU sec, 16.787 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3469, 92289, 3283, 3396) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (670, 1611, 4065) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10855) [M::mem_pestat] mean and std.dev: (2660.69, 2536.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14250) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 378, 487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 857) [M::mem_pestat] mean and std.dev: (395.61, 137.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1042) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (633, 1631, 4138) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11148) [M::mem_pestat] mean and std.dev: (2684.91, 2601.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14653) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (671, 1661, 4018) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10712) [M::mem_pestat] mean and std.dev: (2688.73, 2597.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14059) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 265.951 CPU sec, 16.915 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3375, 92026, 3267, 3510) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (649, 1615, 4271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11515) [M::mem_pestat] mean and std.dev: (2725.95, 2613.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15137) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 379, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860) [M::mem_pestat] mean and std.dev: (396.11, 138.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1046) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (608, 1634, 4137) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11195) [M::mem_pestat] mean and std.dev: (2689.21, 2644.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14724) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (628, 1679, 4179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11281) [M::mem_pestat] mean and std.dev: (2721.44, 2616.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14832) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 264.054 CPU sec, 16.868 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3371, 92387, 3297, 3348) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (630, 1682, 4163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11229) [M::mem_pestat] mean and std.dev: (2692.51, 2611.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14762) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 379, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860) [M::mem_pestat] mean and std.dev: (396.38, 138.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1046) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (642, 1668, 4234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11418) [M::mem_pestat] mean and std.dev: (2740.43, 2640.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15010) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (670, 1654, 4455) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12025) [M::mem_pestat] mean and std.dev: (2796.87, 2685.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15810) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 264.186 CPU sec, 16.867 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3507, 92027, 3213, 3321) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (668, 1730, 4167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11165) [M::mem_pestat] mean and std.dev: (2726.30, 2574.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14664) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (301, 379, 487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 859) [M::mem_pestat] mean and std.dev: (395.63, 137.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1045) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (605, 1620, 4345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11825) [M::mem_pestat] mean and std.dev: (2760.21, 2710.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15565) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (651, 1700, 4089) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10965) [M::mem_pestat] mean and std.dev: (2715.02, 2609.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14403) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 263.577 CPU sec, 16.851 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3460, 92365, 3263, 3521) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (678, 1752, 4209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11271) [M::mem_pestat] mean and std.dev: (2755.43, 2602.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14802) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (301, 378, 487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 859) [M::mem_pestat] mean and std.dev: (395.77, 138.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1045) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (632, 1616, 4066) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10934) [M::mem_pestat] mean and std.dev: (2671.46, 2611.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14368) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (649, 1675, 4018) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10756) [M::mem_pestat] mean and std.dev: (2669.49, 2577.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14125) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 266.388 CPU sec, 16.933 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3301, 91957, 3273, 3496) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (656, 1629, 4165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11183) [M::mem_pestat] mean and std.dev: (2697.73, 2593.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14692) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 380, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861) [M::mem_pestat] mean and std.dev: (397.18, 138.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1047) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (622, 1584, 3915) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10501) [M::mem_pestat] mean and std.dev: (2641.78, 2582.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13794) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (653, 1663, 4030) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10784) [M::mem_pestat] mean and std.dev: (2681.65, 2559.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14161) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 265.120 CPU sec, 16.816 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3439, 91906, 3327, 3403) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (654, 1634, 4139) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11109) [M::mem_pestat] mean and std.dev: (2693.60, 2600.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14594) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 379, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860) [M::mem_pestat] mean and std.dev: (396.55, 137.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1046) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (659, 1774, 4375) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11807) [M::mem_pestat] mean and std.dev: (2816.59, 2689.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15523) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (662, 1685, 4057) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10847) [M::mem_pestat] mean and std.dev: (2709.29, 2587.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14242) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 266.402 CPU sec, 16.962 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3403, 91889, 3222, 3457) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (638, 1663, 4054) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10886) [M::mem_pestat] mean and std.dev: (2676.05, 2548.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14302) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 381, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 869) [M::mem_pestat] mean and std.dev: (398.05, 139.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1058) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (642, 1730, 4477) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12147) [M::mem_pestat] mean and std.dev: (2811.84, 2705.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15982) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (623, 1592, 3972) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10670) [M::mem_pestat] mean and std.dev: (2629.99, 2574.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14019) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 265.576 CPU sec, 16.894 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3532, 92120, 3243, 3497) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (642, 1684, 4214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11358) [M::mem_pestat] mean and std.dev: (2747.26, 2646.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14930) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 379, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 863) [M::mem_pestat] mean and std.dev: (396.50, 138.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1050) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (611, 1815, 4295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11663) [M::mem_pestat] mean and std.dev: (2753.89, 2608.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15347) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (686, 1655, 4133) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11027) [M::mem_pestat] mean and std.dev: (2725.05, 2619.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14474) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 268.732 CPU sec, 17.069 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3433, 92053, 3222, 3295) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (679, 1729, 4126) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11020) [M::mem_pestat] mean and std.dev: (2722.80, 2584.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14467) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 379, 487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 857) [M::mem_pestat] mean and std.dev: (395.62, 136.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1042) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (637, 1752, 4293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11605) [M::mem_pestat] mean and std.dev: (2766.92, 2651.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15261) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (658, 1639, 4085) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10939) [M::mem_pestat] mean and std.dev: (2674.06, 2553.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14366) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 267.753 CPU sec, 17.090 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3364, 91847, 3285, 3492) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (654, 1604, 4111) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11025) [M::mem_pestat] mean and std.dev: (2690.09, 2633.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14482) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 380, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 866) [M::mem_pestat] mean and std.dev: (397.11, 138.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1054) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (599, 1740, 4236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11510) [M::mem_pestat] mean and std.dev: (2706.06, 2596.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15147) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (613, 1587, 3923) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10543) [M::mem_pestat] mean and std.dev: (2602.43, 2549.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13853) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 267.080 CPU sec, 16.948 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3390, 94047, 3213, 3516) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (702, 1751, 4211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11229) [M::mem_pestat] mean and std.dev: (2744.97, 2588.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14738) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (300, 376, 481) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 843) [M::mem_pestat] mean and std.dev: (391.65, 134.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1024) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (633, 1676, 4344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11766) [M::mem_pestat] mean and std.dev: (2732.36, 2636.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15477) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (606, 1557, 3962) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10674) [M::mem_pestat] mean and std.dev: (2641.25, 2590.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14030) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 260.828 CPU sec, 16.689 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3392, 93592, 3315, 3327) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (606, 1607, 4228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11472) [M::mem_pestat] mean and std.dev: (2695.54, 2601.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15094) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (300, 376, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 846) [M::mem_pestat] mean and std.dev: (391.72, 134.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1028) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (653, 1689, 4167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11195) [M::mem_pestat] mean and std.dev: (2727.40, 2647.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14709) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (656, 1649, 4143) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11117) [M::mem_pestat] mean and std.dev: (2708.04, 2617.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14604) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 260.381 CPU sec, 16.503 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3374, 94377, 3349, 3526) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (609, 1538, 3895) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10467) [M::mem_pestat] mean and std.dev: (2616.17, 2567.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13753) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (300, 376, 481) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 843) [M::mem_pestat] mean and std.dev: (391.61, 134.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1024) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (599, 1646, 3996) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10790) [M::mem_pestat] mean and std.dev: (2664.24, 2618.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14187) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (655, 1652, 4119) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11047) [M::mem_pestat] mean and std.dev: (2711.24, 2598.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14511) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 258.934 CPU sec, 16.546 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3433, 93469, 3266, 3463) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (644, 1642, 3988) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10676) [M::mem_pestat] mean and std.dev: (2681.79, 2588.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14020) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (300, 376, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 846) [M::mem_pestat] mean and std.dev: (391.85, 134.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1028) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (651, 1694, 4461) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12081) [M::mem_pestat] mean and std.dev: (2804.36, 2713.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15891) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (657, 1721, 4197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11277) [M::mem_pestat] mean and std.dev: (2738.92, 2595.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14817) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 258.770 CPU sec, 16.393 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3521, 93241, 3294, 3393) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (669, 1715, 4027) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10743) [M::mem_pestat] mean and std.dev: (2739.26, 2599.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14101) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (301, 377, 485) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 853) [M::mem_pestat] mean and std.dev: (393.59, 136.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1037) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (600, 1611, 4240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11520) [M::mem_pestat] mean and std.dev: (2698.87, 2613.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15160) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (635, 1602, 4227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11411) [M::mem_pestat] mean and std.dev: (2734.68, 2681.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15003) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 264.029 CPU sec, 16.887 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3428, 93547, 3240, 3553) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (640, 1693, 4257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11491) [M::mem_pestat] mean and std.dev: (2738.90, 2630.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15108) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (301, 377, 484) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 850) [M::mem_pestat] mean and std.dev: (393.55, 135.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1033) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (598, 1565, 4195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11389) [M::mem_pestat] mean and std.dev: (2653.91, 2632.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14986) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (654, 1622, 4111) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11025) [M::mem_pestat] mean and std.dev: (2687.42, 2607.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14482) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 259.496 CPU sec, 16.474 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3517, 92512, 3342, 3525) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (667, 1618, 4030) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10756) [M::mem_pestat] mean and std.dev: (2668.89, 2566.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14119) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (301, 378, 485) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 853) [M::mem_pestat] mean and std.dev: (393.75, 136.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1037) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (636, 1730, 4131) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11121) [M::mem_pestat] mean and std.dev: (2695.90, 2585.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14616) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (694, 1564, 4091) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10885) [M::mem_pestat] mean and std.dev: (2665.59, 2586.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14282) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 262.850 CPU sec, 16.797 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3337, 92802, 3230, 3614) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (637, 1658, 4119) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11083) [M::mem_pestat] mean and std.dev: (2722.18, 2635.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14565) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (301, 378, 486) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 856) [M::mem_pestat] mean and std.dev: (394.72, 136.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1041) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (619, 1565, 3956) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10630) [M::mem_pestat] mean and std.dev: (2636.09, 2594.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13967) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (664, 1660, 4143) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11101) [M::mem_pestat] mean and std.dev: (2708.03, 2588.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14580) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 259.837 CPU sec, 16.524 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3495, 93037, 3399, 3494) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (640, 1704, 4029) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10807) [M::mem_pestat] mean and std.dev: (2676.05, 2552.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14196) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (301, 379, 486) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 856) [M::mem_pestat] mean and std.dev: (394.37, 135.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1041) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (658, 1671, 3998) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10678) [M::mem_pestat] mean and std.dev: (2667.60, 2587.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14018) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (668, 1696, 4366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11762) [M::mem_pestat] mean and std.dev: (2788.85, 2651.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15460) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 259.615 CPU sec, 16.518 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3378, 92740, 3241, 3458) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (641, 1623, 4121) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11081) [M::mem_pestat] mean and std.dev: (2716.52, 2632.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14561) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 379, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 858) [M::mem_pestat] mean and std.dev: (396.10, 136.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1043) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (633, 1678, 4369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11841) [M::mem_pestat] mean and std.dev: (2813.84, 2713.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15577) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (672, 1592, 3960) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10536) [M::mem_pestat] mean and std.dev: (2629.36, 2549.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13824) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 261.887 CPU sec, 16.679 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3424, 92846, 3374, 3388) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (659, 1664, 4107) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11003) [M::mem_pestat] mean and std.dev: (2693.71, 2583.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14451) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (301, 379, 486) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 856) [M::mem_pestat] mean and std.dev: (394.90, 136.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1041) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (608, 1633, 4236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11492) [M::mem_pestat] mean and std.dev: (2727.23, 2653.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15120) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (620, 1583, 3801) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10163) [M::mem_pestat] mean and std.dev: (2602.68, 2570.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13344) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 262.234 CPU sec, 16.772 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3513, 92928, 3283, 3376) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (615, 1636, 4070) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10980) [M::mem_pestat] mean and std.dev: (2693.20, 2643.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14435) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 379, 487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 857) [M::mem_pestat] mean and std.dev: (395.69, 136.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1042) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (601, 1616, 4082) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11044) [M::mem_pestat] mean and std.dev: (2672.20, 2592.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14525) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (725, 1815, 4107) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10871) [M::mem_pestat] mean and std.dev: (2794.03, 2630.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14253) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 259.728 CPU sec, 16.501 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3523, 92755, 3277, 3516) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (670, 1652, 4164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11152) [M::mem_pestat] mean and std.dev: (2705.45, 2589.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14646) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 380, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860) [M::mem_pestat] mean and std.dev: (396.42, 137.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1046) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (640, 1614, 4075) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10945) [M::mem_pestat] mean and std.dev: (2690.08, 2624.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14380) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (648, 1564, 3989) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10671) [M::mem_pestat] mean and std.dev: (2647.11, 2581.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14012) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 260.648 CPU sec, 16.600 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3318, 92517, 3235, 3403) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (669, 1713, 4082) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10908) [M::mem_pestat] mean and std.dev: (2723.72, 2580.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14321) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 379, 486) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 854) [M::mem_pestat] mean and std.dev: (394.92, 136.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1038) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (639, 1768, 4474) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12144) [M::mem_pestat] mean and std.dev: (2851.78, 2719.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15979) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (679, 1656, 4043) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10771) [M::mem_pestat] mean and std.dev: (2687.79, 2539.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14135) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 260.759 CPU sec, 16.824 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3495, 92497, 3421, 3467) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (636, 1689, 4212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11364) [M::mem_pestat] mean and std.dev: (2763.25, 2665.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14940) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 380, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860) [M::mem_pestat] mean and std.dev: (396.27, 137.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1046) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (631, 1598, 3959) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10615) [M::mem_pestat] mean and std.dev: (2646.51, 2610.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13943) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (643, 1643, 3998) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10708) [M::mem_pestat] mean and std.dev: (2661.32, 2566.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14063) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 260.400 CPU sec, 16.547 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3475, 93152, 3382, 3535) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (654, 1591, 4267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11493) [M::mem_pestat] mean and std.dev: (2722.88, 2668.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15106) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 380, 487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 857) [M::mem_pestat] mean and std.dev: (396.08, 137.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1042) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (612, 1608, 4236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11484) [M::mem_pestat] mean and std.dev: (2723.24, 2672.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15108) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (651, 1618, 4144) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11130) [M::mem_pestat] mean and std.dev: (2728.01, 2651.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14623) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 261.168 CPU sec, 16.871 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3405, 92795, 3382, 3440) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (650, 1630, 4179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11237) [M::mem_pestat] mean and std.dev: (2734.67, 2663.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14766) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 379, 487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 857) [M::mem_pestat] mean and std.dev: (395.05, 136.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1042) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (602, 1586, 4277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11627) [M::mem_pestat] mean and std.dev: (2685.01, 2636.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15302) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (654, 1732, 4303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11601) [M::mem_pestat] mean and std.dev: (2785.26, 2643.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15250) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 261.057 CPU sec, 16.553 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3472, 92620, 3293, 3418) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (624, 1537, 3874) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10374) [M::mem_pestat] mean and std.dev: (2596.59, 2572.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13624) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 380, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860) [M::mem_pestat] mean and std.dev: (396.57, 136.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1046) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (641, 1665, 4062) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10904) [M::mem_pestat] mean and std.dev: (2671.85, 2579.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14325) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (646, 1672, 4129) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11095) [M::mem_pestat] mean and std.dev: (2734.72, 2604.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14578) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 260.306 CPU sec, 16.633 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3463, 92436, 3167, 3505) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (653, 1727, 4209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11321) [M::mem_pestat] mean and std.dev: (2710.14, 2552.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14877) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (301, 379, 487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 859) [M::mem_pestat] mean and std.dev: (395.09, 136.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1045) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (599, 1730, 4195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11387) [M::mem_pestat] mean and std.dev: (2727.60, 2625.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14983) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (674, 1712, 4299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11549) [M::mem_pestat] mean and std.dev: (2772.88, 2626.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15174) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 261.509 CPU sec, 16.621 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3473, 92788, 3295, 3541) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (618, 1594, 4002) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10770) [M::mem_pestat] mean and std.dev: (2630.15, 2537.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14154) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 379, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 863) [M::mem_pestat] mean and std.dev: (396.26, 137.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1050) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (639, 1774, 4426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12000) [M::mem_pestat] mean and std.dev: (2815.23, 2698.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15787) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (663, 1645, 4143) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11103) [M::mem_pestat] mean and std.dev: (2727.60, 2610.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14583) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 260.258 CPU sec, 16.550 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3488, 92829, 3318, 3330) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (672, 1718, 4102) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10962) [M::mem_pestat] mean and std.dev: (2718.53, 2591.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14392) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 379, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860) [M::mem_pestat] mean and std.dev: (396.25, 137.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1046) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (628, 1686, 4486) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12202) [M::mem_pestat] mean and std.dev: (2784.98, 2684.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16060) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (654, 1628, 4350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11742) [M::mem_pestat] mean and std.dev: (2775.04, 2695.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15438) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 259.667 CPU sec, 16.556 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3304, 92622, 3169, 3475) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (642, 1606, 4089) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10983) [M::mem_pestat] mean and std.dev: (2702.37, 2638.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14430) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 379, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861) [M::mem_pestat] mean and std.dev: (396.53, 137.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1047) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (612, 1666, 4268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11580) [M::mem_pestat] mean and std.dev: (2734.98, 2679.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15236) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (665, 1670, 4205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11285) [M::mem_pestat] mean and std.dev: (2733.47, 2621.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14825) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 263.063 CPU sec, 16.745 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3464, 92576, 3395, 3384) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (644, 1657, 3991) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10685) [M::mem_pestat] mean and std.dev: (2645.92, 2524.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14032) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 380, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864) [M::mem_pestat] mean and std.dev: (397.55, 137.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1051) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (642, 1698, 4296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11604) [M::mem_pestat] mean and std.dev: (2776.65, 2662.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15258) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (637, 1678, 4119) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11083) [M::mem_pestat] mean and std.dev: (2719.09, 2604.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14565) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 262.837 CPU sec, 16.735 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3449, 92553, 3254, 3418) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (644, 1622, 4175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11237) [M::mem_pestat] mean and std.dev: (2684.53, 2619.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14768) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 380, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861) [M::mem_pestat] mean and std.dev: (396.80, 137.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1047) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (637, 1778, 4143) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11155) [M::mem_pestat] mean and std.dev: (2732.62, 2595.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14661) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (651, 1727, 4133) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11097) [M::mem_pestat] mean and std.dev: (2740.81, 2626.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14579) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 260.843 CPU sec, 16.607 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3420, 92195, 3164, 3380) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (634, 1606, 3925) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10507) [M::mem_pestat] mean and std.dev: (2693.87, 2639.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13798) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 379, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860) [M::mem_pestat] mean and std.dev: (396.11, 136.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1046) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (585, 1654, 4181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11373) [M::mem_pestat] mean and std.dev: (2704.74, 2642.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14969) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (614, 1516, 3959) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10649) [M::mem_pestat] mean and std.dev: (2572.58, 2542.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13994) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 262.478 CPU sec, 16.728 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3463, 92894, 3249, 3424) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (648, 1568, 4154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11166) [M::mem_pestat] mean and std.dev: (2695.06, 2632.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14672) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 379, 487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 855) [M::mem_pestat] mean and std.dev: (395.93, 137.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1039) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (664, 1691, 4403) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11881) [M::mem_pestat] mean and std.dev: (2780.84, 2687.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15620) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (654, 1634, 3825) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10167) [M::mem_pestat] mean and std.dev: (2612.42, 2535.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13338) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 261.376 CPU sec, 16.656 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3381, 92456, 3192, 3488) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (621, 1503, 3868) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10362) [M::mem_pestat] mean and std.dev: (2597.50, 2577.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13609) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 379, 485) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 851) [M::mem_pestat] mean and std.dev: (394.85, 136.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1034) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (626, 1673, 4104) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11060) [M::mem_pestat] mean and std.dev: (2702.82, 2628.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14538) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (626, 1569, 4025) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10823) [M::mem_pestat] mean and std.dev: (2682.73, 2648.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14222) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 262.629 CPU sec, 16.914 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3547, 92374, 3235, 3325) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (639, 1672, 3864) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10314) [M::mem_pestat] mean and std.dev: (2622.39, 2552.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13539) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 380, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 858) [M::mem_pestat] mean and std.dev: (396.17, 137.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1043) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (604, 1715, 4439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12109) [M::mem_pestat] mean and std.dev: (2832.64, 2758.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15944) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (655, 1703, 4384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11842) [M::mem_pestat] mean and std.dev: (2792.15, 2630.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15571) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 258.870 CPU sec, 16.412 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3409, 93195, 3390, 3372) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (668, 1704, 4132) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11060) [M::mem_pestat] mean and std.dev: (2725.81, 2597.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14524) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 379, 486) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 854) [M::mem_pestat] mean and std.dev: (395.25, 135.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1038) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (660, 1729, 4294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11562) [M::mem_pestat] mean and std.dev: (2769.97, 2657.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15196) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (642, 1689, 3957) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10587) [M::mem_pestat] mean and std.dev: (2686.16, 2600.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13902) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 261.552 CPU sec, 16.673 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3395, 92316, 3235, 3484) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (651, 1704, 4176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11226) [M::mem_pestat] mean and std.dev: (2725.86, 2574.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14751) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 379, 487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 857) [M::mem_pestat] mean and std.dev: (395.79, 137.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1042) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (611, 1692, 4107) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11099) [M::mem_pestat] mean and std.dev: (2700.87, 2601.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14595) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (628, 1645, 3980) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10684) [M::mem_pestat] mean and std.dev: (2644.03, 2534.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14036) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 261.231 CPU sec, 16.834 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3420, 93067, 3343, 3487) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (656, 1741, 4030) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10778) [M::mem_pestat] mean and std.dev: (2700.55, 2560.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14152) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 379, 487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 857) [M::mem_pestat] mean and std.dev: (395.23, 137.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1042) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (578, 1517, 3922) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10610) [M::mem_pestat] mean and std.dev: (2582.38, 2577.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13954) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (624, 1645, 4145) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11187) [M::mem_pestat] mean and std.dev: (2695.44, 2577.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14708) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 261.172 CPU sec, 16.545 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3382, 93037, 3310, 3557) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (623, 1672, 4276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11582) [M::mem_pestat] mean and std.dev: (2697.90, 2580.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15235) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 380, 487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 857) [M::mem_pestat] mean and std.dev: (395.67, 136.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1042) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (629, 1668, 4198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11336) [M::mem_pestat] mean and std.dev: (2724.20, 2645.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14905) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (628, 1565, 3983) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10693) [M::mem_pestat] mean and std.dev: (2613.72, 2550.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14048) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 260.976 CPU sec, 16.610 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3469, 91921, 3340, 3480) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (617, 1488, 3822) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10232) [M::mem_pestat] mean and std.dev: (2589.95, 2582.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13437) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 379, 487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 857) [M::mem_pestat] mean and std.dev: (395.55, 136.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1042) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (626, 1729, 4572) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12464) [M::mem_pestat] mean and std.dev: (2827.52, 2737.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16410) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (662, 1621, 4121) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11039) [M::mem_pestat] mean and std.dev: (2726.44, 2654.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14498) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 260.133 CPU sec, 16.582 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3548, 92589, 3256, 3448) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (693, 1774, 4310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11544) [M::mem_pestat] mean and std.dev: (2834.29, 2652.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15161) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 379, 486) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 854) [M::mem_pestat] mean and std.dev: (395.19, 137.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1038) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (649, 1657, 4341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11725) [M::mem_pestat] mean and std.dev: (2730.52, 2642.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15417) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (648, 1668, 3990) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10674) [M::mem_pestat] mean and std.dev: (2677.77, 2597.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14016) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 261.992 CPU sec, 16.643 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3438, 93067, 3205, 3520) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (656, 1660, 4183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11237) [M::mem_pestat] mean and std.dev: (2771.51, 2683.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14764) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 380, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860) [M::mem_pestat] mean and std.dev: (396.83, 137.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1046) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (645, 1697, 4295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11595) [M::mem_pestat] mean and std.dev: (2733.76, 2632.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15245) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (658, 1537, 3925) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10459) [M::mem_pestat] mean and std.dev: (2615.71, 2570.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13726) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 261.809 CPU sec, 16.847 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3552, 92003, 3411, 3284) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (650, 1677, 4235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11405) [M::mem_pestat] mean and std.dev: (2751.67, 2662.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14990) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 379, 486) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 854) [M::mem_pestat] mean and std.dev: (395.08, 136.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1038) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (613, 1618, 4075) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10999) [M::mem_pestat] mean and std.dev: (2670.22, 2618.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14461) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (668, 1682, 4376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11792) [M::mem_pestat] mean and std.dev: (2810.32, 2710.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15500) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 262.570 CPU sec, 16.667 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3494, 92597, 3231, 3382) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (666, 1609, 4052) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10824) [M::mem_pestat] mean and std.dev: (2673.35, 2586.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14210) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 378, 487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 857) [M::mem_pestat] mean and std.dev: (395.70, 136.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1042) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (632, 1741, 4238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11450) [M::mem_pestat] mean and std.dev: (2752.59, 2664.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15056) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (670, 1612, 4208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11284) [M::mem_pestat] mean and std.dev: (2718.89, 2609.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14822) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 264.046 CPU sec, 16.820 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3413, 93058, 3143, 3411) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (672, 1706, 4030) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10746) [M::mem_pestat] mean and std.dev: (2675.88, 2549.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14104) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 379, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860) [M::mem_pestat] mean and std.dev: (396.22, 137.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1046) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (600, 1613, 4243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11529) [M::mem_pestat] mean and std.dev: (2698.17, 2648.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15172) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (623, 1685, 4368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11858) [M::mem_pestat] mean and std.dev: (2779.73, 2672.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15603) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 261.329 CPU sec, 16.648 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3463, 92933, 3269, 3445) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (650, 1607, 4126) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11078) [M::mem_pestat] mean and std.dev: (2673.91, 2579.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14554) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (301, 379, 487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 859) [M::mem_pestat] mean and std.dev: (395.80, 137.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1045) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (672, 1814, 4285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11511) [M::mem_pestat] mean and std.dev: (2758.87, 2585.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15124) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (658, 1670, 4090) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10954) [M::mem_pestat] mean and std.dev: (2689.52, 2581.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14386) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 263.157 CPU sec, 16.740 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3567, 92387, 3312, 3403) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (641, 1614, 4005) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10733) [M::mem_pestat] mean and std.dev: (2652.56, 2571.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14097) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 379, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 863) [M::mem_pestat] mean and std.dev: (395.93, 137.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1050) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (601, 1605, 4345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11833) [M::mem_pestat] mean and std.dev: (2714.42, 2658.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15577) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (669, 1624, 4258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11436) [M::mem_pestat] mean and std.dev: (2723.23, 2619.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15025) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 264.103 CPU sec, 17.066 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3498, 92709, 3252, 3434) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (622, 1605, 3901) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10459) [M::mem_pestat] mean and std.dev: (2638.53, 2573.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13738) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 380, 487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 855) [M::mem_pestat] mean and std.dev: (396.28, 136.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1039) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (617, 1626, 4300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11666) [M::mem_pestat] mean and std.dev: (2739.64, 2661.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15349) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (666, 1692, 4279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11505) [M::mem_pestat] mean and std.dev: (2769.80, 2649.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15118) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 263.546 CPU sec, 16.813 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3522, 92800, 3287, 3521) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (645, 1584, 4040) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10830) [M::mem_pestat] mean and std.dev: (2673.23, 2601.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14225) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 380, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 863) [M::mem_pestat] mean and std.dev: (396.99, 138.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1050) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (650, 1707, 4328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11684) [M::mem_pestat] mean and std.dev: (2746.29, 2627.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15362) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (629, 1603, 3975) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10667) [M::mem_pestat] mean and std.dev: (2632.44, 2547.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14013) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 264.009 CPU sec, 16.764 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3506, 92901, 3194, 3401) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (624, 1617, 4160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11232) [M::mem_pestat] mean and std.dev: (2690.41, 2615.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14768) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 380, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860) [M::mem_pestat] mean and std.dev: (395.97, 137.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1046) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (675, 1859, 4299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11547) [M::mem_pestat] mean and std.dev: (2813.76, 2621.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15171) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (659, 1686, 4017) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10733) [M::mem_pestat] mean and std.dev: (2684.59, 2565.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14091) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 264.728 CPU sec, 16.849 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3451, 92956, 3418, 3405) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (630, 1595, 4181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11283) [M::mem_pestat] mean and std.dev: (2689.52, 2601.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14834) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 378, 486) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 854) [M::mem_pestat] mean and std.dev: (394.88, 136.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1038) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (655, 1744, 4273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11509) [M::mem_pestat] mean and std.dev: (2744.79, 2600.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15127) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (651, 1632, 4012) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10734) [M::mem_pestat] mean and std.dev: (2677.07, 2575.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14095) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 262.768 CPU sec, 16.709 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3481, 92711, 3279, 3396) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (634, 1588, 4005) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10747) [M::mem_pestat] mean and std.dev: (2646.53, 2578.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14118) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 379, 487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 857) [M::mem_pestat] mean and std.dev: (395.78, 137.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1042) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (619, 1724, 4387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11923) [M::mem_pestat] mean and std.dev: (2763.43, 2656.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15691) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (622, 1552, 3844) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10288) [M::mem_pestat] mean and std.dev: (2597.85, 2552.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13510) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 263.683 CPU sec, 16.947 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3359, 92749, 3307, 3414) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (659, 1688, 4033) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10781) [M::mem_pestat] mean and std.dev: (2655.92, 2517.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14155) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (301, 379, 487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 859) [M::mem_pestat] mean and std.dev: (394.96, 137.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1045) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (613, 1723, 4367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11875) [M::mem_pestat] mean and std.dev: (2756.35, 2660.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15629) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (630, 1668, 4311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11673) [M::mem_pestat] mean and std.dev: (2752.72, 2636.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15354) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 267.144 CPU sec, 16.982 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3430, 92301, 3247, 3364) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (649, 1678, 4102) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11008) [M::mem_pestat] mean and std.dev: (2703.92, 2601.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14461) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 379, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 863) [M::mem_pestat] mean and std.dev: (396.97, 138.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1050) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (629, 1656, 4386) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11900) [M::mem_pestat] mean and std.dev: (2772.40, 2691.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15657) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (681, 1720, 3981) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10581) [M::mem_pestat] mean and std.dev: (2661.35, 2503.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13881) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 263.003 CPU sec, 16.909 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3414, 92245, 3266, 3439) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (671, 1687, 3940) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10478) [M::mem_pestat] mean and std.dev: (2673.85, 2547.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13747) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 379, 487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 857) [M::mem_pestat] mean and std.dev: (395.77, 136.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1042) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (595, 1620, 4032) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10906) [M::mem_pestat] mean and std.dev: (2704.93, 2698.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14343) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (627, 1636, 4097) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11037) [M::mem_pestat] mean and std.dev: (2715.84, 2620.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14507) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 264.928 CPU sec, 16.890 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3381, 92178, 3354, 3310) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (666, 1661, 4207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11289) [M::mem_pestat] mean and std.dev: (2744.98, 2628.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14830) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 380, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 858) [M::mem_pestat] mean and std.dev: (396.72, 137.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1043) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (650, 1685, 4215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11345) [M::mem_pestat] mean and std.dev: (2737.47, 2635.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14910) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (649, 1624, 4182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11248) [M::mem_pestat] mean and std.dev: (2720.22, 2627.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14781) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 265.739 CPU sec, 16.961 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3589, 92342, 3305, 3436) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (669, 1752, 4267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11463) [M::mem_pestat] mean and std.dev: (2769.83, 2619.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15061) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 379, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860) [M::mem_pestat] mean and std.dev: (396.07, 137.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1046) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (646, 1761, 4344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11740) [M::mem_pestat] mean and std.dev: (2784.60, 2683.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15438) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (633, 1634, 4011) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10767) [M::mem_pestat] mean and std.dev: (2658.00, 2573.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14145) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 265.946 CPU sec, 16.986 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3528, 92658, 3213, 3412) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (650, 1689, 4038) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10814) [M::mem_pestat] mean and std.dev: (2678.43, 2569.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14202) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 379, 487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 857) [M::mem_pestat] mean and std.dev: (395.14, 136.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1042) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (569, 1579, 3856) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10430) [M::mem_pestat] mean and std.dev: (2534.57, 2538.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13717) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (636, 1585, 4045) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10863) [M::mem_pestat] mean and std.dev: (2664.00, 2583.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14272) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 265.636 CPU sec, 16.932 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3404, 92473, 3332, 3464) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (672, 1708, 4128) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11040) [M::mem_pestat] mean and std.dev: (2695.39, 2560.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14496) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 379, 486) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 854) [M::mem_pestat] mean and std.dev: (395.29, 136.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1038) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (646, 1694, 4146) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11146) [M::mem_pestat] mean and std.dev: (2702.11, 2599.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14646) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (607, 1681, 3923) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10555) [M::mem_pestat] mean and std.dev: (2622.88, 2538.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13871) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 263.828 CPU sec, 16.822 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3452, 92269, 3189, 3426) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (619, 1622, 4166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11260) [M::mem_pestat] mean and std.dev: (2697.63, 2628.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14807) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (301, 378, 485) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 853) [M::mem_pestat] mean and std.dev: (394.47, 136.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1037) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (611, 1663, 4101) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11081) [M::mem_pestat] mean and std.dev: (2708.19, 2637.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14571) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (636, 1586, 4201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11331) [M::mem_pestat] mean and std.dev: (2682.29, 2585.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14896) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 266.187 CPU sec, 16.892 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3407, 93146, 3245, 3467) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (612, 1605, 3900) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10476) [M::mem_pestat] mean and std.dev: (2623.02, 2562.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13764) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (301, 377, 485) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 853) [M::mem_pestat] mean and std.dev: (394.18, 136.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1037) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (600, 1560, 4026) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10878) [M::mem_pestat] mean and std.dev: (2634.05, 2613.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14304) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (660, 1647, 4173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11199) [M::mem_pestat] mean and std.dev: (2707.51, 2602.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14712) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 266.184 CPU sec, 16.931 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3347, 92563, 3273, 3377) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] (25, 50, 75) percentile: (639, 1666, 4004) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10734) [M::mem_pestat] mean and std.dev: (2672.37, 2560.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14099) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 378, 485) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 851) [M::mem_pestat] mean and std.dev: (394.41, 135.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1034) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (640, 1766, 4307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11641) [M::mem_pestat] mean and std.dev: (2752.58, 2626.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15308) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (635, 1606, 3962) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10616) [M::mem_pestat] mean and std.dev: (2631.33, 2537.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13943) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 265.982 CPU sec, 16.943 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3481, 92862, 3192, 3435) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (658, 1624, 3988) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10648) [M::mem_pestat] mean and std.dev: (2654.32, 2567.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13978) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (301, 377, 484) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 850) [M::mem_pestat] mean and std.dev: (393.59, 135.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1033) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (587, 1690, 4177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11357) [M::mem_pestat] mean and std.dev: (2706.32, 2647.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14947) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (646, 1583, 4058) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10882) [M::mem_pestat] mean and std.dev: (2628.22, 2530.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14294) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 267.786 CPU sec, 17.080 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3512, 92146, 3085, 3424) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (635, 1577, 4094) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11012) [M::mem_pestat] mean and std.dev: (2647.09, 2560.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14471) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (301, 377, 486) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 856) [M::mem_pestat] mean and std.dev: (394.75, 136.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1041) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (642, 1720, 4126) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11094) [M::mem_pestat] mean and std.dev: (2721.79, 2632.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14578) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (680, 1683, 4274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11462) [M::mem_pestat] mean and std.dev: (2748.01, 2612.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15056) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 267.546 CPU sec, 17.212 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3377, 92421, 3273, 3520) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (634, 1638, 4053) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10891) [M::mem_pestat] mean and std.dev: (2666.07, 2579.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14310) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (301, 378, 486) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 856) [M::mem_pestat] mean and std.dev: (394.66, 137.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1041) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (602, 1745, 4298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11690) [M::mem_pestat] mean and std.dev: (2725.98, 2612.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15386) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (638, 1609, 4103) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11033) [M::mem_pestat] mean and std.dev: (2661.50, 2572.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14498) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 267.794 CPU sec, 16.984 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3371, 93324, 3277, 3366) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (639, 1589, 4045) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10857) [M::mem_pestat] mean and std.dev: (2684.10, 2625.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14263) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 378, 486) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 854) [M::mem_pestat] mean and std.dev: (394.40, 135.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1038) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (612, 1664, 4167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11277) [M::mem_pestat] mean and std.dev: (2697.65, 2632.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14832) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (643, 1545, 4011) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10747) [M::mem_pestat] mean and std.dev: (2673.38, 2622.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14115) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 268.514 CPU sec, 17.203 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3448, 91778, 3143, 3487) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (658, 1630, 3969) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10591) [M::mem_pestat] mean and std.dev: (2609.59, 2487.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13902) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 380, 487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 855) [M::mem_pestat] mean and std.dev: (396.14, 136.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1039) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (567, 1627, 3997) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10857) [M::mem_pestat] mean and std.dev: (2597.49, 2542.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14287) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (637, 1671, 4029) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10813) [M::mem_pestat] mean and std.dev: (2688.78, 2609.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14205) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 266.748 CPU sec, 17.020 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3445, 92649, 3341, 3438) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (641, 1591, 3972) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10634) [M::mem_pestat] mean and std.dev: (2654.38, 2582.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13965) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 378, 485) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 851) [M::mem_pestat] mean and std.dev: (394.16, 135.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1034) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (636, 1689, 4028) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10812) [M::mem_pestat] mean and std.dev: (2688.74, 2597.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14204) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (631, 1625, 3937) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10549) [M::mem_pestat] mean and std.dev: (2706.66, 2663.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13855) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 267.771 CPU sec, 17.258 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3428, 92759, 3197, 3426) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (629, 1668, 4129) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11129) [M::mem_pestat] mean and std.dev: (2693.09, 2588.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14629) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (301, 377, 485) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 853) [M::mem_pestat] mean and std.dev: (394.14, 135.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1037) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (632, 1631, 4193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11315) [M::mem_pestat] mean and std.dev: (2699.44, 2636.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14876) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (643, 1663, 4191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11287) [M::mem_pestat] mean and std.dev: (2745.22, 2665.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14835) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 263.940 CPU sec, 16.734 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3357, 92930, 3303, 3377) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (680, 1718, 4099) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10937) [M::mem_pestat] mean and std.dev: (2724.13, 2611.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14356) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (300, 378, 485) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 855) [M::mem_pestat] mean and std.dev: (394.02, 136.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1040) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (650, 1678, 4253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11459) [M::mem_pestat] mean and std.dev: (2763.89, 2653.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15062) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (658, 1728, 4175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11209) [M::mem_pestat] mean and std.dev: (2728.03, 2579.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14726) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 264.237 CPU sec, 16.955 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3564, 92272, 3374, 3437) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (646, 1636, 3954) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10570) [M::mem_pestat] mean and std.dev: (2657.73, 2587.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13878) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 380, 487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 857) [M::mem_pestat] mean and std.dev: (395.55, 136.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1042) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (626, 1632, 4167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11249) [M::mem_pestat] mean and std.dev: (2659.21, 2542.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14790) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (633, 1560, 4156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11202) [M::mem_pestat] mean and std.dev: (2684.23, 2623.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14725) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 265.461 CPU sec, 16.869 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3585, 92054, 3262, 3434) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (607, 1559, 4035) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10891) [M::mem_pestat] mean and std.dev: (2669.33, 2633.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14319) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 379, 487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 855) [M::mem_pestat] mean and std.dev: (395.77, 135.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1039) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (635, 1726, 4065) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10925) [M::mem_pestat] mean and std.dev: (2692.79, 2609.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14355) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (645, 1557, 4017) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10761) [M::mem_pestat] mean and std.dev: (2650.84, 2595.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14133) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 265.128 CPU sec, 17.047 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3419, 92490, 3371, 3361) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (627, 1550, 3932) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10542) [M::mem_pestat] mean and std.dev: (2619.99, 2565.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13847) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 379, 487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 855) [M::mem_pestat] mean and std.dev: (395.66, 135.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1039) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (629, 1726, 4426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12020) [M::mem_pestat] mean and std.dev: (2810.92, 2668.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15817) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (666, 1754, 4306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11586) [M::mem_pestat] mean and std.dev: (2787.28, 2654.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15226) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 264.183 CPU sec, 16.716 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3482, 92428, 3328, 3536) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (621, 1589, 3950) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10608) [M::mem_pestat] mean and std.dev: (2663.89, 2634.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13937) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 378, 486) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 854) [M::mem_pestat] mean and std.dev: (394.17, 135.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1038) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (634, 1622, 3922) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10498) [M::mem_pestat] mean and std.dev: (2626.45, 2569.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13786) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (658, 1654, 4265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11479) [M::mem_pestat] mean and std.dev: (2732.85, 2611.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15086) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 264.984 CPU sec, 16.971 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3391, 91800, 3165, 3378) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (682, 1699, 4247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11377) [M::mem_pestat] mean and std.dev: (2769.49, 2639.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14942) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 379, 487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 857) [M::mem_pestat] mean and std.dev: (396.13, 136.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1042) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (615, 1731, 4155) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11235) [M::mem_pestat] mean and std.dev: (2714.93, 2617.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14775) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (649, 1569, 4057) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10873) [M::mem_pestat] mean and std.dev: (2653.57, 2579.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14281) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 266.862 CPU sec, 16.928 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3567, 92098, 3366, 3483) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (647, 1673, 4099) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11003) [M::mem_pestat] mean and std.dev: (2734.29, 2640.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14455) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (301, 378, 485) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 853) [M::mem_pestat] mean and std.dev: (394.54, 135.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1037) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (593, 1659, 4165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11309) [M::mem_pestat] mean and std.dev: (2674.71, 2609.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14881) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (674, 1674, 3896) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10340) [M::mem_pestat] mean and std.dev: (2653.99, 2535.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13562) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 266.308 CPU sec, 16.959 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3564, 91812, 3235, 3464) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (647, 1680, 3889) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10373) [M::mem_pestat] mean and std.dev: (2635.09, 2540.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13615) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (304, 381, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 859) [M::mem_pestat] mean and std.dev: (397.48, 136.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1044) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (637, 1717, 4351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11779) [M::mem_pestat] mean and std.dev: (2766.66, 2663.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15493) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (677, 1655, 4186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11204) [M::mem_pestat] mean and std.dev: (2730.68, 2607.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14713) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 265.190 CPU sec, 16.999 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3414, 92629, 3285, 3421) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (640, 1662, 4275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11545) [M::mem_pestat] mean and std.dev: (2733.21, 2634.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15180) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 380, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861) [M::mem_pestat] mean and std.dev: (397.10, 137.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1047) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (631, 1672, 4064) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10930) [M::mem_pestat] mean and std.dev: (2695.76, 2631.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14363) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (666, 1610, 3879) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10305) [M::mem_pestat] mean and std.dev: (2601.59, 2512.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13518) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 265.388 CPU sec, 16.865 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3517, 91279, 3285, 3463) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (632, 1649, 4323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11705) [M::mem_pestat] mean and std.dev: (2749.15, 2638.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15396) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 380, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861) [M::mem_pestat] mean and std.dev: (396.69, 137.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1047) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (642, 1726, 4134) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11118) [M::mem_pestat] mean and std.dev: (2692.86, 2587.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14610) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (644, 1599, 4042) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10838) [M::mem_pestat] mean and std.dev: (2654.96, 2568.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14236) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 261.857 CPU sec, 16.680 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3407, 91688, 3365, 3444) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (596, 1527, 4091) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11081) [M::mem_pestat] mean and std.dev: (2627.97, 2597.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14576) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (304, 382, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 865) [M::mem_pestat] mean and std.dev: (398.70, 137.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1052) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (627, 1721, 4241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11469) [M::mem_pestat] mean and std.dev: (2722.15, 2607.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15083) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (654, 1654, 4122) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11058) [M::mem_pestat] mean and std.dev: (2664.16, 2549.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14526) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 262.919 CPU sec, 16.725 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3480, 91504, 3243, 3475) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (678, 1674, 4468) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12048) [M::mem_pestat] mean and std.dev: (2812.21, 2696.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15838) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 382, 492) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 870) [M::mem_pestat] mean and std.dev: (398.49, 139.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1059) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (635, 1714, 4245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11465) [M::mem_pestat] mean and std.dev: (2763.61, 2667.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15075) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (626, 1580, 4151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11201) [M::mem_pestat] mean and std.dev: (2718.81, 2670.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14726) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 263.413 CPU sec, 16.774 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3325, 91568, 3248, 3460) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (668, 1661, 4061) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10847) [M::mem_pestat] mean and std.dev: (2702.46, 2595.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14240) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (304, 380, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 856) [M::mem_pestat] mean and std.dev: (396.92, 136.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1040) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (596, 1554, 4109) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11135) [M::mem_pestat] mean and std.dev: (2628.65, 2602.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14648) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (654, 1644, 4145) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11127) [M::mem_pestat] mean and std.dev: (2701.83, 2594.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14618) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 263.255 CPU sec, 16.776 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3502, 91760, 3341, 3458) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (639, 1599, 4179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11259) [M::mem_pestat] mean and std.dev: (2727.21, 2656.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14799) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (304, 381, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 862) [M::mem_pestat] mean and std.dev: (397.88, 136.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1048) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (604, 1617, 3978) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10726) [M::mem_pestat] mean and std.dev: (2601.59, 2552.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14100) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (639, 1621, 3883) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10371) [M::mem_pestat] mean and std.dev: (2631.67, 2550.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13615) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 266.694 CPU sec, 16.986 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3416, 92005, 3250, 3455) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (630, 1587, 4016) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10788) [M::mem_pestat] mean and std.dev: (2672.55, 2613.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14174) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 381, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864) [M::mem_pestat] mean and std.dev: (397.31, 137.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1051) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (611, 1604, 4095) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11063) [M::mem_pestat] mean and std.dev: (2673.38, 2629.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14547) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (678, 1672, 4124) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11016) [M::mem_pestat] mean and std.dev: (2679.18, 2564.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14462) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 267.105 CPU sec, 17.102 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3316, 92093, 3297, 3502) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (646, 1607, 3955) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10573) [M::mem_pestat] mean and std.dev: (2645.50, 2548.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13882) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 381, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861) [M::mem_pestat] mean and std.dev: (396.70, 136.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1047) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (635, 1648, 4181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11273) [M::mem_pestat] mean and std.dev: (2689.55, 2634.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14819) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (654, 1646, 4203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11301) [M::mem_pestat] mean and std.dev: (2732.74, 2613.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14850) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 265.433 CPU sec, 17.057 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3372, 91679, 3300, 3445) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (680, 1742, 4081) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10883) [M::mem_pestat] mean and std.dev: (2720.08, 2598.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14284) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 381, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864) [M::mem_pestat] mean and std.dev: (397.31, 137.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1051) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (625, 1650, 4039) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10867) [M::mem_pestat] mean and std.dev: (2672.01, 2617.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14281) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (672, 1684, 4222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11322) [M::mem_pestat] mean and std.dev: (2742.32, 2643.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14872) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 263.848 CPU sec, 16.847 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3499, 91782, 3273, 3407) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (679, 1745, 4120) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11002) [M::mem_pestat] mean and std.dev: (2721.46, 2546.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14443) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (304, 381, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 859) [M::mem_pestat] mean and std.dev: (397.77, 136.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1044) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (643, 1753, 4273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11533) [M::mem_pestat] mean and std.dev: (2770.00, 2637.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15163) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (628, 1596, 3960) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10624) [M::mem_pestat] mean and std.dev: (2639.62, 2566.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13956) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 266.518 CPU sec, 17.145 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3622, 91011, 3383, 3553) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (639, 1533, 3890) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10392) [M::mem_pestat] mean and std.dev: (2618.21, 2590.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13643) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (304, 382, 492) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 868) [M::mem_pestat] mean and std.dev: (398.82, 137.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1056) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (632, 1673, 4147) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11177) [M::mem_pestat] mean and std.dev: (2718.89, 2632.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14692) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (611, 1573, 4088) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11042) [M::mem_pestat] mean and std.dev: (2655.25, 2594.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14519) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 269.980 CPU sec, 17.162 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3489, 91277, 3316, 3428) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (665, 1598, 4027) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10751) [M::mem_pestat] mean and std.dev: (2638.60, 2543.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14113) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 381, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864) [M::mem_pestat] mean and std.dev: (397.60, 137.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1051) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (641, 1662, 3960) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10598) [M::mem_pestat] mean and std.dev: (2672.54, 2592.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13917) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (671, 1816, 4487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12119) [M::mem_pestat] mean and std.dev: (2842.84, 2671.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15935) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 268.294 CPU sec, 17.056 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3326, 91396, 3400, 3555) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (651, 1654, 4180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11238) [M::mem_pestat] mean and std.dev: (2706.89, 2573.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14767) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 382, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 867) [M::mem_pestat] mean and std.dev: (398.72, 139.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1055) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (629, 1714, 4284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11594) [M::mem_pestat] mean and std.dev: (2741.55, 2654.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15249) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (682, 1664, 4233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11335) [M::mem_pestat] mean and std.dev: (2754.90, 2643.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14886) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 270.124 CPU sec, 17.178 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3447, 91492, 3291, 3452) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (657, 1638, 4201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11289) [M::mem_pestat] mean and std.dev: (2714.56, 2628.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14833) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (304, 381, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 865) [M::mem_pestat] mean and std.dev: (398.04, 137.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1052) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (609, 1620, 4172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11298) [M::mem_pestat] mean and std.dev: (2673.78, 2613.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14861) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (657, 1655, 4115) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11031) [M::mem_pestat] mean and std.dev: (2679.26, 2575.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14489) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 269.646 CPU sec, 17.174 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3489, 91472, 3187, 3412) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (685, 1677, 4045) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10765) [M::mem_pestat] mean and std.dev: (2684.71, 2571.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14125) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (304, 381, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 862) [M::mem_pestat] mean and std.dev: (397.85, 137.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1048) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (644, 1668, 4118) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11066) [M::mem_pestat] mean and std.dev: (2715.65, 2633.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14540) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (641, 1614, 4038) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10832) [M::mem_pestat] mean and std.dev: (2654.33, 2574.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14229) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 269.561 CPU sec, 17.237 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3353, 92372, 3183, 3332) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (692, 1789, 4364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11708) [M::mem_pestat] mean and std.dev: (2833.38, 2689.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15380) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (304, 380, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 862) [M::mem_pestat] mean and std.dev: (397.25, 136.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1048) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (628, 1731, 4221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11407) [M::mem_pestat] mean and std.dev: (2736.98, 2636.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15000) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (661, 1619, 4011) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10711) [M::mem_pestat] mean and std.dev: (2648.97, 2551.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14061) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 275.295 CPU sec, 17.948 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3447, 91119, 3354, 3440) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (631, 1580, 4095) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11023) [M::mem_pestat] mean and std.dev: (2686.68, 2622.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14487) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (304, 380, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 856) [M::mem_pestat] mean and std.dev: (396.46, 135.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1040) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (604, 1694, 4358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11866) [M::mem_pestat] mean and std.dev: (2723.70, 2623.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15620) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (602, 1670, 4186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11354) [M::mem_pestat] mean and std.dev: (2714.95, 2621.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14938) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 269.659 CPU sec, 17.099 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3372, 91338, 3300, 3369) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (664, 1694, 4157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11143) [M::mem_pestat] mean and std.dev: (2716.50, 2586.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14636) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (305, 382, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 863) [M::mem_pestat] mean and std.dev: (399.03, 137.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1049) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (609, 1681, 4270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11592) [M::mem_pestat] mean and std.dev: (2716.47, 2648.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15253) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (654, 1686, 4119) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11049) [M::mem_pestat] mean and std.dev: (2724.50, 2626.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14514) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 274.593 CPU sec, 17.679 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3382, 91374, 3402, 3420) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (661, 1661, 4189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11245) [M::mem_pestat] mean and std.dev: (2706.36, 2576.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14773) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (304, 380, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 856) [M::mem_pestat] mean and std.dev: (397.15, 136.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1040) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (590, 1668, 4259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11597) [M::mem_pestat] mean and std.dev: (2684.44, 2618.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15266) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (622, 1736, 4092) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11032) [M::mem_pestat] mean and std.dev: (2709.89, 2584.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14502) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 276.620 CPU sec, 17.529 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3332, 91653, 3244, 3490) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (647, 1648, 4046) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10844) [M::mem_pestat] mean and std.dev: (2672.38, 2569.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14243) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 380, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860) [M::mem_pestat] mean and std.dev: (396.54, 137.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1046) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (616, 1679, 4326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11746) [M::mem_pestat] mean and std.dev: (2749.71, 2670.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15456) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (670, 1700, 4064) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10852) [M::mem_pestat] mean and std.dev: (2704.55, 2553.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14246) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 270.576 CPU sec, 17.281 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3412, 91769, 3345, 3489) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (637, 1511, 3865) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10321) [M::mem_pestat] mean and std.dev: (2595.86, 2568.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13549) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 381, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864) [M::mem_pestat] mean and std.dev: (397.21, 137.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1051) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (643, 1739, 4225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11389) [M::mem_pestat] mean and std.dev: (2750.35, 2636.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14971) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (601, 1585, 4062) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10984) [M::mem_pestat] mean and std.dev: (2665.72, 2623.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14445) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 271.529 CPU sec, 17.252 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3449, 92253, 3192, 3568) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (663, 1694, 3980) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10614) [M::mem_pestat] mean and std.dev: (2697.16, 2602.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13931) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (304, 381, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 862) [M::mem_pestat] mean and std.dev: (397.65, 136.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1048) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (625, 1644, 4108) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11074) [M::mem_pestat] mean and std.dev: (2664.45, 2572.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14557) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (637, 1585, 4172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11242) [M::mem_pestat] mean and std.dev: (2701.03, 2611.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14777) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 269.223 CPU sec, 17.136 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3473, 92550, 3337, 3380) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (681, 1680, 4034) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10740) [M::mem_pestat] mean and std.dev: (2713.47, 2598.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14093) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 380, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861) [M::mem_pestat] mean and std.dev: (396.58, 137.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1047) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (627, 1703, 4098) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11040) [M::mem_pestat] mean and std.dev: (2706.60, 2619.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14511) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (604, 1552, 4108) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11116) [M::mem_pestat] mean and std.dev: (2686.94, 2651.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14620) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 268.232 CPU sec, 17.067 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3398, 91870, 3233, 3478) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (649, 1692, 4270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11512) [M::mem_pestat] mean and std.dev: (2736.18, 2612.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15133) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 379, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860) [M::mem_pestat] mean and std.dev: (396.24, 137.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1046) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (621, 1697, 4186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11316) [M::mem_pestat] mean and std.dev: (2730.94, 2619.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14881) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (637, 1687, 4268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11530) [M::mem_pestat] mean and std.dev: (2745.28, 2642.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15161) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 266.688 CPU sec, 16.910 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3455, 91680, 3409, 3422) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (676, 1704, 4126) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11026) [M::mem_pestat] mean and std.dev: (2713.55, 2578.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14476) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 380, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 858) [M::mem_pestat] mean and std.dev: (396.65, 136.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1043) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (659, 1792, 4396) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11870) [M::mem_pestat] mean and std.dev: (2759.44, 2601.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15607) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (627, 1633, 4307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11667) [M::mem_pestat] mean and std.dev: (2723.80, 2612.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15347) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 269.351 CPU sec, 17.157 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3559, 91982, 3350, 3509) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (677, 1724, 4099) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10943) [M::mem_pestat] mean and std.dev: (2692.06, 2549.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14365) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 380, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864) [M::mem_pestat] mean and std.dev: (397.84, 137.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1051) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (660, 1799, 4443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12009) [M::mem_pestat] mean and std.dev: (2850.78, 2709.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15792) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (659, 1662, 4156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11150) [M::mem_pestat] mean and std.dev: (2721.39, 2595.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14647) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 269.037 CPU sec, 17.177 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3424, 91429, 3225, 3361) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (659, 1663, 4079) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10919) [M::mem_pestat] mean and std.dev: (2685.01, 2567.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14339) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 379, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860) [M::mem_pestat] mean and std.dev: (396.18, 137.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1046) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (634, 1717, 4149) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11179) [M::mem_pestat] mean and std.dev: (2714.03, 2590.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14694) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (663, 1649, 4132) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11070) [M::mem_pestat] mean and std.dev: (2711.51, 2601.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14539) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 270.158 CPU sec, 17.228 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3390, 92495, 3381, 3287) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (634, 1608, 4115) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11077) [M::mem_pestat] mean and std.dev: (2716.38, 2671.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14558) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 380, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 858) [M::mem_pestat] mean and std.dev: (396.47, 136.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1043) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (579, 1638, 4121) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11205) [M::mem_pestat] mean and std.dev: (2657.95, 2575.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14747) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (624, 1625, 4004) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10764) [M::mem_pestat] mean and std.dev: (2660.89, 2585.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14144) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 270.472 CPU sec, 17.176 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3550, 91561, 3341, 3417) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (636, 1689, 4104) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11040) [M::mem_pestat] mean and std.dev: (2701.20, 2593.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14508) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 380, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 858) [M::mem_pestat] mean and std.dev: (397.01, 137.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1043) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (642, 1748, 4371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11829) [M::mem_pestat] mean and std.dev: (2797.14, 2675.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15558) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (656, 1684, 4170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11198) [M::mem_pestat] mean and std.dev: (2756.76, 2627.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14712) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 272.426 CPU sec, 17.441 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3403, 91247, 3455, 3513) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (667, 1642, 4132) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11062) [M::mem_pestat] mean and std.dev: (2709.39, 2592.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14527) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 380, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860) [M::mem_pestat] mean and std.dev: (396.03, 136.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1046) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (637, 1675, 4122) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11092) [M::mem_pestat] mean and std.dev: (2697.49, 2617.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14577) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (675, 1685, 4069) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10857) [M::mem_pestat] mean and std.dev: (2699.39, 2589.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14251) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 273.527 CPU sec, 17.357 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3310, 91237, 3306, 3329) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (640, 1679, 4007) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10741) [M::mem_pestat] mean and std.dev: (2685.45, 2583.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14108) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 380, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 858) [M::mem_pestat] mean and std.dev: (396.21, 136.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1043) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (576, 1684, 4452) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12204) [M::mem_pestat] mean and std.dev: (2749.24, 2691.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16080) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (645, 1713, 4259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11487) [M::mem_pestat] mean and std.dev: (2744.70, 2602.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15101) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 271.592 CPU sec, 17.292 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3325, 91917, 3305, 3558) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (660, 1670, 4189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11247) [M::mem_pestat] mean and std.dev: (2724.03, 2607.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14776) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 380, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 858) [M::mem_pestat] mean and std.dev: (396.45, 136.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1043) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (622, 1738, 4486) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12214) [M::mem_pestat] mean and std.dev: (2808.11, 2717.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16078) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (665, 1671, 4082) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10916) [M::mem_pestat] mean and std.dev: (2710.26, 2582.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14333) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 267.084 CPU sec, 17.180 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3356, 91798, 3339, 3526) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (662, 1609, 4084) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10928) [M::mem_pestat] mean and std.dev: (2705.33, 2636.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14350) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 380, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861) [M::mem_pestat] mean and std.dev: (396.73, 137.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1047) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (601, 1579, 4129) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11185) [M::mem_pestat] mean and std.dev: (2693.46, 2665.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14713) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (662, 1656, 4006) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10694) [M::mem_pestat] mean and std.dev: (2657.78, 2562.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14038) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 271.531 CPU sec, 17.204 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3508, 92294, 3203, 3562) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (628, 1637, 4135) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11149) [M::mem_pestat] mean and std.dev: (2701.43, 2631.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14656) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 381, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 858) [M::mem_pestat] mean and std.dev: (396.92, 137.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1043) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (631, 1690, 4160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11218) [M::mem_pestat] mean and std.dev: (2689.78, 2579.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14747) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (637, 1673, 4120) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11086) [M::mem_pestat] mean and std.dev: (2730.26, 2651.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14569) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 270.033 CPU sec, 17.275 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3403, 92183, 3364, 3456) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (673, 1728, 4275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11479) [M::mem_pestat] mean and std.dev: (2763.27, 2609.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15081) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 379, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860) [M::mem_pestat] mean and std.dev: (396.04, 136.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1046) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (593, 1630, 4075) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11039) [M::mem_pestat] mean and std.dev: (2666.56, 2619.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14521) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (637, 1661, 4277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11557) [M::mem_pestat] mean and std.dev: (2740.67, 2622.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15197) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 268.995 CPU sec, 17.103 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3465, 91621, 3386, 3406) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (649, 1625, 4033) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10801) [M::mem_pestat] mean and std.dev: (2639.41, 2558.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14185) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 380, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860) [M::mem_pestat] mean and std.dev: (396.24, 136.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1046) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (612, 1575, 4138) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11190) [M::mem_pestat] mean and std.dev: (2637.56, 2576.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14716) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (647, 1626, 4168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11210) [M::mem_pestat] mean and std.dev: (2717.16, 2636.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14731) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 270.175 CPU sec, 17.263 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3573, 91902, 3325, 3487) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (678, 1708, 4101) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10947) [M::mem_pestat] mean and std.dev: (2716.50, 2593.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14370) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 380, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860) [M::mem_pestat] mean and std.dev: (396.58, 137.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1046) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (635, 1738, 4269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11537) [M::mem_pestat] mean and std.dev: (2720.92, 2594.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15171) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (662, 1651, 4164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11168) [M::mem_pestat] mean and std.dev: (2735.24, 2620.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14670) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 270.232 CPU sec, 17.300 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3331, 92247, 3314, 3412) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (696, 1718, 3966) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10506) [M::mem_pestat] mean and std.dev: (2695.28, 2585.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13776) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 379, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861) [M::mem_pestat] mean and std.dev: (396.72, 137.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1047) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (622, 1691, 4173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11275) [M::mem_pestat] mean and std.dev: (2703.89, 2588.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14826) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (675, 1656, 4130) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11040) [M::mem_pestat] mean and std.dev: (2703.54, 2574.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14495) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 268.342 CPU sec, 17.068 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3487, 91360, 3171, 3380) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (643, 1628, 3789) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10081) [M::mem_pestat] mean and std.dev: (2630.62, 2570.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13227) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 380, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860) [M::mem_pestat] mean and std.dev: (396.30, 137.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1046) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (610, 1749, 4402) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11986) [M::mem_pestat] mean and std.dev: (2767.75, 2629.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15778) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (654, 1638, 4174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11214) [M::mem_pestat] mean and std.dev: (2694.26, 2591.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14734) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 269.972 CPU sec, 17.378 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3492, 91598, 3179, 3427) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (636, 1637, 3949) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10575) [M::mem_pestat] mean and std.dev: (2656.42, 2550.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13888) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 380, 487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 855) [M::mem_pestat] mean and std.dev: (396.32, 136.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1039) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (618, 1685, 4218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11418) [M::mem_pestat] mean and std.dev: (2713.93, 2615.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15018) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (679, 1699, 4236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11350) [M::mem_pestat] mean and std.dev: (2772.69, 2640.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14907) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 265.527 CPU sec, 16.954 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3470, 91926, 3249, 3426) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (646, 1637, 4287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11569) [M::mem_pestat] mean and std.dev: (2744.41, 2644.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15210) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 379, 487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 857) [M::mem_pestat] mean and std.dev: (396.07, 136.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1042) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (593, 1620, 4027) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10895) [M::mem_pestat] mean and std.dev: (2659.15, 2631.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14329) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (638, 1630, 4062) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10910) [M::mem_pestat] mean and std.dev: (2677.60, 2580.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14334) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 265.837 CPU sec, 17.096 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3548, 92131, 3252, 3392) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (635, 1709, 4191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11303) [M::mem_pestat] mean and std.dev: (2728.19, 2612.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14859) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 380, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 858) [M::mem_pestat] mean and std.dev: (396.33, 137.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1043) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (644, 1690, 4276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11540) [M::mem_pestat] mean and std.dev: (2745.67, 2640.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15172) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (625, 1570, 3935) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10555) [M::mem_pestat] mean and std.dev: (2626.34, 2601.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13865) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 266.795 CPU sec, 16.982 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3439, 91891, 3265, 3411) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (630, 1602, 4107) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11061) [M::mem_pestat] mean and std.dev: (2684.85, 2625.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14538) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 379, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 858) [M::mem_pestat] mean and std.dev: (396.66, 137.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1043) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (647, 1585, 3984) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10658) [M::mem_pestat] mean and std.dev: (2601.13, 2511.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13995) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (655, 1573, 4001) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10693) [M::mem_pestat] mean and std.dev: (2636.98, 2556.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14039) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 266.968 CPU sec, 17.105 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3395, 91979, 3356, 3416) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (628, 1609, 4058) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10918) [M::mem_pestat] mean and std.dev: (2634.20, 2557.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14348) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 378, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860) [M::mem_pestat] mean and std.dev: (395.72, 137.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1046) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (606, 1666, 4255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11553) [M::mem_pestat] mean and std.dev: (2727.38, 2639.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15202) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (660, 1689, 4251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11433) [M::mem_pestat] mean and std.dev: (2712.20, 2603.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15024) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 267.390 CPU sec, 16.984 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3438, 91543, 3406, 3462) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (656, 1708, 4134) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11090) [M::mem_pestat] mean and std.dev: (2749.16, 2649.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14568) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 380, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 863) [M::mem_pestat] mean and std.dev: (396.43, 137.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1050) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (591, 1596, 4239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11535) [M::mem_pestat] mean and std.dev: (2678.35, 2627.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15183) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (627, 1655, 3932) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10542) [M::mem_pestat] mean and std.dev: (2661.11, 2583.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13847) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 266.890 CPU sec, 16.983 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3440, 91793, 3320, 3533) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (616, 1603, 4060) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10948) [M::mem_pestat] mean and std.dev: (2656.91, 2616.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14392) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 379, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860) [M::mem_pestat] mean and std.dev: (396.17, 137.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1046) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (613, 1623, 4084) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11026) [M::mem_pestat] mean and std.dev: (2645.19, 2593.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14497) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (648, 1740, 4400) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11904) [M::mem_pestat] mean and std.dev: (2777.28, 2644.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15656) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 268.460 CPU sec, 17.078 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3350, 92257, 3395, 3426) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (661, 1695, 4288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11542) [M::mem_pestat] mean and std.dev: (2754.17, 2624.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15169) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 380, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864) [M::mem_pestat] mean and std.dev: (397.15, 138.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1051) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (562, 1590, 4095) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11161) [M::mem_pestat] mean and std.dev: (2664.37, 2669.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14694) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (671, 1698, 4151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11111) [M::mem_pestat] mean and std.dev: (2736.10, 2626.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14591) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 268.088 CPU sec, 17.076 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3364, 92433, 3300, 3534) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (641, 1707, 4306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11636) [M::mem_pestat] mean and std.dev: (2771.36, 2636.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15301) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (301, 377, 485) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 853) [M::mem_pestat] mean and std.dev: (393.79, 136.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1037) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (606, 1685, 4131) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11181) [M::mem_pestat] mean and std.dev: (2670.56, 2586.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14706) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (665, 1613, 3997) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10661) [M::mem_pestat] mean and std.dev: (2676.97, 2589.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13993) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 269.374 CPU sec, 17.511 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3348, 91650, 3332, 3411) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (639, 1738, 4148) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11166) [M::mem_pestat] mean and std.dev: (2741.55, 2618.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14675) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 379, 487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 857) [M::mem_pestat] mean and std.dev: (395.93, 136.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1042) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (642, 1711, 4199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11313) [M::mem_pestat] mean and std.dev: (2734.60, 2625.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14870) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (611, 1524, 3926) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10556) [M::mem_pestat] mean and std.dev: (2632.93, 2629.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13871) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 273.674 CPU sec, 17.383 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3440, 91655, 3318, 3366) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (592, 1541, 4083) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11065) [M::mem_pestat] mean and std.dev: (2660.81, 2642.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14556) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 379, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861) [M::mem_pestat] mean and std.dev: (396.64, 137.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1047) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (608, 1593, 4113) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11123) [M::mem_pestat] mean and std.dev: (2662.97, 2615.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14628) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (636, 1619, 4154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11190) [M::mem_pestat] mean and std.dev: (2651.86, 2553.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14708) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 266.318 CPU sec, 16.932 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3291, 92273, 3275, 3438) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (659, 1695, 4195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11267) [M::mem_pestat] mean and std.dev: (2770.92, 2654.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14803) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (301, 378, 487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 859) [M::mem_pestat] mean and std.dev: (395.02, 136.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1045) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (585, 1622, 4030) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10920) [M::mem_pestat] mean and std.dev: (2632.72, 2598.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14365) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (673, 1684, 4000) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10654) [M::mem_pestat] mean and std.dev: (2690.66, 2571.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13981) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 267.778 CPU sec, 17.080 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3488, 92077, 3180, 3374) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (600, 1453, 4146) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11238) [M::mem_pestat] mean and std.dev: (2634.87, 2618.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14784) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 381, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 858) [M::mem_pestat] mean and std.dev: (396.22, 136.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1043) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (598, 1595, 4072) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11020) [M::mem_pestat] mean and std.dev: (2675.59, 2642.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14494) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (647, 1592, 3943) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10535) [M::mem_pestat] mean and std.dev: (2612.58, 2523.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13831) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 266.991 CPU sec, 16.943 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3361, 92051, 3351, 3476) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (644, 1607, 4214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11354) [M::mem_pestat] mean and std.dev: (2729.09, 2668.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14924) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (301, 379, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 865) [M::mem_pestat] mean and std.dev: (395.63, 137.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1053) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (702, 1836, 4248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11340) [M::mem_pestat] mean and std.dev: (2774.86, 2599.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14886) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (635, 1558, 4225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11405) [M::mem_pestat] mean and std.dev: (2694.78, 2643.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14995) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 265.102 CPU sec, 16.864 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3518, 92020, 3418, 3511) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (687, 1649, 4022) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10692) [M::mem_pestat] mean and std.dev: (2686.01, 2583.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14027) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 380, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860) [M::mem_pestat] mean and std.dev: (396.13, 137.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1046) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (613, 1739, 4451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12127) [M::mem_pestat] mean and std.dev: (2784.12, 2652.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15965) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (660, 1705, 4093) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10959) [M::mem_pestat] mean and std.dev: (2720.24, 2596.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14392) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 260.864 CPU sec, 16.616 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3513, 91919, 3381, 3485) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (659, 1658, 4316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11630) [M::mem_pestat] mean and std.dev: (2795.35, 2701.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15287) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 380, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861) [M::mem_pestat] mean and std.dev: (396.78, 137.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1047) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (607, 1597, 4138) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11200) [M::mem_pestat] mean and std.dev: (2683.33, 2635.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14731) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (658, 1692, 4326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11662) [M::mem_pestat] mean and std.dev: (2767.24, 2624.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15330) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 263.606 CPU sec, 16.795 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3401, 92382, 3323, 3357) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (621, 1608, 4235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11463) [M::mem_pestat] mean and std.dev: (2710.35, 2629.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15077) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 380, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 858) [M::mem_pestat] mean and std.dev: (396.76, 137.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1043) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (613, 1645, 4135) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11179) [M::mem_pestat] mean and std.dev: (2668.33, 2615.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14701) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (685, 1603, 4151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11083) [M::mem_pestat] mean and std.dev: (2753.66, 2669.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14549) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 264.281 CPU sec, 16.836 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3461, 92638, 3352, 3465) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (636, 1628, 4237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11439) [M::mem_pestat] mean and std.dev: (2714.57, 2620.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15040) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 380, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 866) [M::mem_pestat] mean and std.dev: (397.28, 138.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1054) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (625, 1583, 4329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11737) [M::mem_pestat] mean and std.dev: (2686.13, 2601.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15441) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (633, 1646, 4101) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11037) [M::mem_pestat] mean and std.dev: (2702.65, 2625.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14505) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 267.306 CPU sec, 17.084 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3516, 92088, 3211, 3397) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (643, 1629, 4027) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10795) [M::mem_pestat] mean and std.dev: (2683.53, 2609.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14179) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 381, 492) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 870) [M::mem_pestat] mean and std.dev: (397.96, 138.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1059) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (605, 1660, 4265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11585) [M::mem_pestat] mean and std.dev: (2731.99, 2690.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15245) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] (25, 50, 75) percentile: (604, 1520, 3901) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10495) [M::mem_pestat] mean and std.dev: (2573.98, 2540.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13792) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 262.891 CPU sec, 16.794 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3202, 92036, 3309, 3348) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (631, 1638, 4265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11533) [M::mem_pestat] mean and std.dev: (2726.72, 2613.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15167) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 380, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861) [M::mem_pestat] mean and std.dev: (396.91, 137.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1047) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (652, 1733, 4311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11629) [M::mem_pestat] mean and std.dev: (2771.69, 2649.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15288) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (663, 1565, 3944) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10506) [M::mem_pestat] mean and std.dev: (2613.48, 2527.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13787) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 264.861 CPU sec, 17.001 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3558, 92503, 3284, 3463) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (606, 1648, 4067) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10989) [M::mem_pestat] mean and std.dev: (2691.63, 2647.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14450) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 382, 492) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 870) [M::mem_pestat] mean and std.dev: (398.71, 138.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1059) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (624, 1629, 4258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11526) [M::mem_pestat] mean and std.dev: (2726.58, 2686.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15160) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (659, 1573, 3804) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10094) [M::mem_pestat] mean and std.dev: (2581.97, 2506.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13239) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 267.906 CPU sec, 17.048 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3375, 92008, 3310, 3406) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (609, 1631, 4081) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11025) [M::mem_pestat] mean and std.dev: (2690.51, 2623.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14497) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (304, 381, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 865) [M::mem_pestat] mean and std.dev: (398.23, 138.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1052) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (610, 1697, 4079) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11017) [M::mem_pestat] mean and std.dev: (2689.90, 2601.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14486) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (638, 1589, 3903) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10433) [M::mem_pestat] mean and std.dev: (2612.34, 2522.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13698) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 262.816 CPU sec, 16.734 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3358, 92221, 3261, 3452) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (640, 1555, 4063) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10909) [M::mem_pestat] mean and std.dev: (2651.98, 2587.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14332) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (304, 382, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 865) [M::mem_pestat] mean and std.dev: (398.50, 137.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1052) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (628, 1762, 4385) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11899) [M::mem_pestat] mean and std.dev: (2808.81, 2712.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15656) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (648, 1678, 4103) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11013) [M::mem_pestat] mean and std.dev: (2699.09, 2598.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14468) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 262.501 CPU sec, 16.732 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3386, 91915, 3207, 3335) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (673, 1704, 4102) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10960) [M::mem_pestat] mean and std.dev: (2700.02, 2558.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14389) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 381, 493) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 873) [M::mem_pestat] mean and std.dev: (398.59, 139.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1063) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (631, 1620, 3990) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10708) [M::mem_pestat] mean and std.dev: (2627.62, 2566.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14067) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (682, 1651, 4317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11587) [M::mem_pestat] mean and std.dev: (2721.73, 2582.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15222) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 264.711 CPU sec, 17.018 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3545, 91889, 3326, 3437) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (630, 1607, 3993) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10719) [M::mem_pestat] mean and std.dev: (2631.49, 2561.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14082) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (304, 382, 492) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 868) [M::mem_pestat] mean and std.dev: (398.72, 138.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1056) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (623, 1711, 3978) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10688) [M::mem_pestat] mean and std.dev: (2643.62, 2539.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14043) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (642, 1646, 4120) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11076) [M::mem_pestat] mean and std.dev: (2738.15, 2657.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14554) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 265.410 CPU sec, 16.853 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3563, 91721, 3245, 3455) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (624, 1574, 4047) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10893) [M::mem_pestat] mean and std.dev: (2638.00, 2554.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14316) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (304, 381, 492) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 868) [M::mem_pestat] mean and std.dev: (398.56, 139.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1056) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (653, 1720, 4258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11468) [M::mem_pestat] mean and std.dev: (2751.76, 2634.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15073) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (659, 1529, 4159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11159) [M::mem_pestat] mean and std.dev: (2669.99, 2606.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14659) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 264.230 CPU sec, 16.794 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3490, 91524, 3380, 3434) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (660, 1656, 4169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11187) [M::mem_pestat] mean and std.dev: (2743.51, 2654.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14696) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (304, 382, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 865) [M::mem_pestat] mean and std.dev: (398.59, 137.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1052) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (645, 1663, 4231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11403) [M::mem_pestat] mean and std.dev: (2717.23, 2631.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14989) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (642, 1698, 4108) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11040) [M::mem_pestat] mean and std.dev: (2696.03, 2573.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14506) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 265.686 CPU sec, 16.899 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3443, 91478, 3312, 3396) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (643, 1656, 4108) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11038) [M::mem_pestat] mean and std.dev: (2682.12, 2550.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14503) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 382, 492) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 870) [M::mem_pestat] mean and std.dev: (399.68, 139.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1059) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (592, 1657, 4188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11380) [M::mem_pestat] mean and std.dev: (2716.11, 2680.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14976) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (634, 1631, 4060) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10912) [M::mem_pestat] mean and std.dev: (2626.87, 2518.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14338) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 267.095 CPU sec, 16.962 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3425, 91517, 3405, 3416) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (660, 1677, 4091) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10953) [M::mem_pestat] mean and std.dev: (2702.00, 2584.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14384) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (304, 382, 492) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 868) [M::mem_pestat] mean and std.dev: (398.92, 138.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1056) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (621, 1700, 4391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11931) [M::mem_pestat] mean and std.dev: (2795.13, 2717.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15701) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (598, 1552, 3989) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10771) [M::mem_pestat] mean and std.dev: (2611.88, 2566.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14162) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 265.562 CPU sec, 17.006 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3374, 91986, 3376, 3494) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (660, 1557, 3974) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10602) [M::mem_pestat] mean and std.dev: (2605.16, 2508.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13916) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (305, 383, 493) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 869) [M::mem_pestat] mean and std.dev: (400.14, 139.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1057) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (589, 1512, 3858) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10396) [M::mem_pestat] mean and std.dev: (2575.20, 2557.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13665) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (649, 1659, 4119) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11059) [M::mem_pestat] mean and std.dev: (2716.19, 2599.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14529) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 265.057 CPU sec, 16.830 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3439, 91246, 3334, 3473) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (639, 1639, 3956) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10590) [M::mem_pestat] mean and std.dev: (2629.32, 2529.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13907) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (305, 382, 493) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 869) [M::mem_pestat] mean and std.dev: (399.45, 139.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1057) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (578, 1561, 4217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11495) [M::mem_pestat] mean and std.dev: (2645.15, 2608.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15134) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (638, 1646, 4007) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10745) [M::mem_pestat] mean and std.dev: (2663.68, 2569.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14114) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 264.398 CPU sec, 16.818 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3540, 91269, 3166, 3320) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (630, 1614, 4040) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10860) [M::mem_pestat] mean and std.dev: (2633.34, 2541.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14270) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (304, 382, 493) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 871) [M::mem_pestat] mean and std.dev: (399.44, 138.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1060) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (620, 1771, 4240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11480) [M::mem_pestat] mean and std.dev: (2748.04, 2604.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15100) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (652, 1659, 4121) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11059) [M::mem_pestat] mean and std.dev: (2738.65, 2611.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14528) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 264.124 CPU sec, 16.803 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3570, 91509, 3270, 3417) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (644, 1574, 3917) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10463) [M::mem_pestat] mean and std.dev: (2640.25, 2555.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13736) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 381, 495) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 879) [M::mem_pestat] mean and std.dev: (399.55, 140.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1071) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (602, 1644, 4059) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10973) [M::mem_pestat] mean and std.dev: (2661.52, 2613.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14430) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (636, 1588, 4098) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11022) [M::mem_pestat] mean and std.dev: (2661.45, 2579.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14484) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 266.982 CPU sec, 17.030 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3411, 91088, 3283, 3508) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (667, 1661, 4090) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10936) [M::mem_pestat] mean and std.dev: (2715.80, 2597.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14359) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 382, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 867) [M::mem_pestat] mean and std.dev: (398.20, 138.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1055) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (609, 1734, 4238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11496) [M::mem_pestat] mean and std.dev: (2730.02, 2654.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15125) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (667, 1676, 4251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11419) [M::mem_pestat] mean and std.dev: (2723.91, 2588.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15003) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 266.078 CPU sec, 16.874 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3375, 91489, 3247, 3341) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (641, 1628, 4099) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11015) [M::mem_pestat] mean and std.dev: (2693.79, 2612.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14473) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (304, 382, 493) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 871) [M::mem_pestat] mean and std.dev: (399.53, 138.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1060) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (614, 1667, 4043) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10901) [M::mem_pestat] mean and std.dev: (2657.90, 2560.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14330) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (638, 1629, 4128) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11108) [M::mem_pestat] mean and std.dev: (2673.25, 2574.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14598) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 266.574 CPU sec, 16.965 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3356, 91366, 3362, 3364) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (678, 1648, 4144) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11076) [M::mem_pestat] mean and std.dev: (2724.18, 2633.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14542) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (304, 382, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 862) [M::mem_pestat] mean and std.dev: (398.02, 137.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1048) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (582, 1640, 3852) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10392) [M::mem_pestat] mean and std.dev: (2639.59, 2609.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13662) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (633, 1569, 3971) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10647) [M::mem_pestat] mean and std.dev: (2664.12, 2595.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13985) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 267.339 CPU sec, 17.298 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3325, 91336, 3338, 3492) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (605, 1541, 4145) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11225) [M::mem_pestat] mean and std.dev: (2651.48, 2637.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14765) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (304, 383, 493) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 871) [M::mem_pestat] mean and std.dev: (399.22, 138.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1060) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (600, 1665, 4257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11571) [M::mem_pestat] mean and std.dev: (2707.94, 2626.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15228) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (610, 1588, 3949) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10627) [M::mem_pestat] mean and std.dev: (2631.09, 2570.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13966) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 266.445 CPU sec, 16.958 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3455, 91149, 3368, 3542) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (630, 1670, 4228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11424) [M::mem_pestat] mean and std.dev: (2733.01, 2612.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15022) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (305, 383, 493) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 869) [M::mem_pestat] mean and std.dev: (399.11, 138.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1057) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (656, 1710, 3979) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10625) [M::mem_pestat] mean and std.dev: (2662.42, 2568.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13948) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (653, 1746, 4166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11192) [M::mem_pestat] mean and std.dev: (2705.98, 2565.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14705) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 271.338 CPU sec, 17.278 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3493, 91421, 3312, 3423) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (677, 1698, 4076) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10874) [M::mem_pestat] mean and std.dev: (2725.83, 2587.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14273) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (305, 383, 493) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 869) [M::mem_pestat] mean and std.dev: (400.02, 138.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1057) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (636, 1677, 3970) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10638) [M::mem_pestat] mean and std.dev: (2688.12, 2635.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13972) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (637, 1639, 4221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11389) [M::mem_pestat] mean and std.dev: (2721.93, 2618.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14973) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 269.275 CPU sec, 17.278 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3391, 90463, 3351, 3569) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (613, 1686, 4307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11695) [M::mem_pestat] mean and std.dev: (2754.81, 2629.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15389) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (305, 382, 492) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 866) [M::mem_pestat] mean and std.dev: (399.84, 138.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1053) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (614, 1648, 4120) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11132) [M::mem_pestat] mean and std.dev: (2696.58, 2614.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14638) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (631, 1643, 4032) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10834) [M::mem_pestat] mean and std.dev: (2648.77, 2549.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14235) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 268.599 CPU sec, 17.041 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3486, 91213, 3326, 3498) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (640, 1622, 4126) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11098) [M::mem_pestat] mean and std.dev: (2689.69, 2616.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14584) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (304, 382, 492) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 868) [M::mem_pestat] mean and std.dev: (399.13, 138.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1056) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (627, 1608, 4447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12087) [M::mem_pestat] mean and std.dev: (2751.72, 2685.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15907) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (681, 1703, 4221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11301) [M::mem_pestat] mean and std.dev: (2763.35, 2613.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14841) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 271.124 CPU sec, 17.216 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3535, 91310, 3256, 3472) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (629, 1658, 4077) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10973) [M::mem_pestat] mean and std.dev: (2709.09, 2616.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14421) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (304, 382, 492) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 868) [M::mem_pestat] mean and std.dev: (399.03, 138.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1056) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (633, 1688, 4203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11343) [M::mem_pestat] mean and std.dev: (2755.22, 2696.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14913) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (660, 1717, 4026) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10758) [M::mem_pestat] mean and std.dev: (2717.26, 2575.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14124) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 267.561 CPU sec, 17.271 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3481, 90712, 3434, 3506) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (655, 1732, 4345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11725) [M::mem_pestat] mean and std.dev: (2739.15, 2558.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15415) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (304, 382, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 865) [M::mem_pestat] mean and std.dev: (399.05, 138.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1052) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (597, 1693, 4299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11703) [M::mem_pestat] mean and std.dev: (2761.56, 2698.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15405) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (612, 1615, 4029) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10863) [M::mem_pestat] mean and std.dev: (2662.97, 2581.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14280) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 268.008 CPU sec, 17.092 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3433, 91333, 3331, 3421) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (657, 1694, 4142) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11112) [M::mem_pestat] mean and std.dev: (2720.69, 2600.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14597) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (304, 382, 492) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 868) [M::mem_pestat] mean and std.dev: (399.08, 138.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1056) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (588, 1688, 4188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11388) [M::mem_pestat] mean and std.dev: (2678.64, 2589.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14988) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (650, 1683, 3983) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10649) [M::mem_pestat] mean and std.dev: (2658.01, 2551.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13982) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 271.515 CPU sec, 17.451 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3389, 91368, 3465, 3572) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (665, 1658, 4227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11351) [M::mem_pestat] mean and std.dev: (2734.21, 2630.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14913) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (304, 382, 492) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 868) [M::mem_pestat] mean and std.dev: (398.91, 138.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1056) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (602, 1640, 4200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11396) [M::mem_pestat] mean and std.dev: (2736.51, 2683.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14994) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (632, 1629, 4111) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11069) [M::mem_pestat] mean and std.dev: (2704.32, 2645.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14548) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 272.286 CPU sec, 17.506 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3350, 91339, 3254, 3505) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (681, 1746, 4383) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11787) [M::mem_pestat] mean and std.dev: (2786.70, 2615.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15489) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 382, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864) [M::mem_pestat] mean and std.dev: (398.53, 138.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1051) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (620, 1638, 4301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11663) [M::mem_pestat] mean and std.dev: (2761.10, 2702.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15344) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (631, 1682, 4166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11236) [M::mem_pestat] mean and std.dev: (2739.36, 2636.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14771) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 273.767 CPU sec, 17.501 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3509, 90665, 3255, 3363) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (628, 1564, 4014) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10786) [M::mem_pestat] mean and std.dev: (2643.59, 2582.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14172) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (304, 382, 492) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 868) [M::mem_pestat] mean and std.dev: (399.19, 138.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1056) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (603, 1698, 4321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11757) [M::mem_pestat] mean and std.dev: (2748.45, 2676.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15475) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (673, 1633, 3978) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10588) [M::mem_pestat] mean and std.dev: (2653.55, 2559.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13893) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 271.577 CPU sec, 17.262 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3418, 91527, 3361, 3455) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (660, 1720, 4112) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11016) [M::mem_pestat] mean and std.dev: (2699.44, 2558.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14468) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (304, 382, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 865) [M::mem_pestat] mean and std.dev: (399.02, 138.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1052) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (619, 1663, 4132) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11158) [M::mem_pestat] mean and std.dev: (2676.86, 2594.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14671) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (658, 1651, 4111) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11017) [M::mem_pestat] mean and std.dev: (2714.74, 2608.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14470) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 275.360 CPU sec, 18.100 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3527, 91737, 3276, 3406) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (646, 1597, 4154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11170) [M::mem_pestat] mean and std.dev: (2706.26, 2633.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14678) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 381, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 869) [M::mem_pestat] mean and std.dev: (398.22, 139.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1058) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (636, 1794, 4331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11721) [M::mem_pestat] mean and std.dev: (2749.64, 2612.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15416) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (691, 1720, 4273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11437) [M::mem_pestat] mean and std.dev: (2767.52, 2635.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15019) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 269.039 CPU sec, 17.135 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3446, 91994, 3277, 3462) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (678, 1701, 4182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11190) [M::mem_pestat] mean and std.dev: (2705.76, 2552.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14694) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 380, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 866) [M::mem_pestat] mean and std.dev: (397.48, 137.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1054) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (583, 1553, 4167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11335) [M::mem_pestat] mean and std.dev: (2661.27, 2635.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14919) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (656, 1707, 4048) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10832) [M::mem_pestat] mean and std.dev: (2672.46, 2523.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14224) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 268.147 CPU sec, 17.106 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3445, 91239, 3252, 3365) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (607, 1573, 3979) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10723) [M::mem_pestat] mean and std.dev: (2608.77, 2574.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14095) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (304, 381, 492) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 868) [M::mem_pestat] mean and std.dev: (398.48, 138.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1056) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (592, 1725, 4396) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12004) [M::mem_pestat] mean and std.dev: (2746.09, 2653.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15808) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (624, 1669, 4191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11325) [M::mem_pestat] mean and std.dev: (2744.69, 2652.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14892) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 267.246 CPU sec, 16.961 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3435, 91926, 3361, 3541) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (656, 1656, 3971) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10601) [M::mem_pestat] mean and std.dev: (2679.09, 2595.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13916) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 381, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 858) [M::mem_pestat] mean and std.dev: (396.79, 136.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1043) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (616, 1638, 4175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11293) [M::mem_pestat] mean and std.dev: (2697.01, 2632.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14852) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (645, 1648, 4081) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10953) [M::mem_pestat] mean and std.dev: (2688.69, 2590.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14389) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 270.131 CPU sec, 17.262 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3258, 91522, 3249, 3539) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (666, 1644, 4230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11358) [M::mem_pestat] mean and std.dev: (2727.65, 2620.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14922) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (304, 382, 493) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 871) [M::mem_pestat] mean and std.dev: (399.54, 138.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1060) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (617, 1694, 4194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11348) [M::mem_pestat] mean and std.dev: (2708.57, 2638.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14925) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (663, 1693, 3939) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10491) [M::mem_pestat] mean and std.dev: (2679.27, 2549.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13767) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 267.161 CPU sec, 17.002 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3311, 91150, 3320, 3329) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (619, 1513, 3960) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10642) [M::mem_pestat] mean and std.dev: (2645.22, 2616.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13983) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 382, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 867) [M::mem_pestat] mean and std.dev: (398.64, 138.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1055) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (647, 1800, 4332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11702) [M::mem_pestat] mean and std.dev: (2775.32, 2630.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15387) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (660, 1724, 4353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11739) [M::mem_pestat] mean and std.dev: (2789.03, 2641.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15432) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 270.345 CPU sec, 17.339 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3480, 91397, 3283, 3466) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (666, 1765, 4388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11832) [M::mem_pestat] mean and std.dev: (2836.69, 2682.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15554) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 380, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 867) [M::mem_pestat] mean and std.dev: (398.22, 138.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1055) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (617, 1673, 4264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11558) [M::mem_pestat] mean and std.dev: (2747.33, 2648.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15205) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (627, 1656, 3903) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10455) [M::mem_pestat] mean and std.dev: (2626.62, 2536.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13731) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 266.784 CPU sec, 16.919 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3276, 91249, 3382, 3531) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (649, 1666, 4109) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11029) [M::mem_pestat] mean and std.dev: (2718.67, 2621.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14489) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (304, 382, 492) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 868) [M::mem_pestat] mean and std.dev: (399.17, 139.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1056) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (643, 1686, 4179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11251) [M::mem_pestat] mean and std.dev: (2724.47, 2611.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14787) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (671, 1720, 4287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11519) [M::mem_pestat] mean and std.dev: (2786.91, 2646.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15135) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 266.910 CPU sec, 17.108 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3336, 92037, 3332, 3443) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (640, 1699, 4110) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11050) [M::mem_pestat] mean and std.dev: (2742.50, 2630.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14520) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 380, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864) [M::mem_pestat] mean and std.dev: (397.41, 138.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1051) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (602, 1653, 4096) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11084) [M::mem_pestat] mean and std.dev: (2686.37, 2628.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14578) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (636, 1735, 4181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11271) [M::mem_pestat] mean and std.dev: (2731.39, 2612.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14816) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 265.666 CPU sec, 16.854 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3456, 91605, 3267, 3391) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (659, 1730, 4384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11834) [M::mem_pestat] mean and std.dev: (2800.45, 2659.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15559) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 380, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 866) [M::mem_pestat] mean and std.dev: (397.14, 138.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1054) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (607, 1633, 4424) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12058) [M::mem_pestat] mean and std.dev: (2764.23, 2702.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15875) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (680, 1694, 4181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11183) [M::mem_pestat] mean and std.dev: (2747.56, 2616.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14684) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 269.274 CPU sec, 17.160 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3430, 91353, 3334, 3330) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (632, 1668, 3975) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10661) [M::mem_pestat] mean and std.dev: (2689.23, 2610.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14004) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 381, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 867) [M::mem_pestat] mean and std.dev: (397.93, 137.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1055) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (652, 1734, 4358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11770) [M::mem_pestat] mean and std.dev: (2805.41, 2705.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15476) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (649, 1672, 4090) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10972) [M::mem_pestat] mean and std.dev: (2715.76, 2626.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14413) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 269.475 CPU sec, 17.173 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3416, 91801, 3209, 3435) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (655, 1730, 4101) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10993) [M::mem_pestat] mean and std.dev: (2718.39, 2595.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14439) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (304, 381, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 862) [M::mem_pestat] mean and std.dev: (397.56, 137.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1048) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (591, 1625, 4052) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10974) [M::mem_pestat] mean and std.dev: (2656.87, 2631.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14435) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (653, 1672, 4060) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10874) [M::mem_pestat] mean and std.dev: (2697.94, 2596.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14281) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 267.514 CPU sec, 17.099 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3361, 91475, 3260, 3366) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (639, 1635, 4134) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11124) [M::mem_pestat] mean and std.dev: (2671.88, 2563.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14619) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 380, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864) [M::mem_pestat] mean and std.dev: (397.56, 137.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1051) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (644, 1729, 4325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11687) [M::mem_pestat] mean and std.dev: (2753.44, 2644.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15368) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (649, 1688, 4281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11545) [M::mem_pestat] mean and std.dev: (2795.84, 2709.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15177) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 268.575 CPU sec, 17.317 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3411, 91337, 3284, 3368) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (629, 1631, 4324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11714) [M::mem_pestat] mean and std.dev: (2761.53, 2672.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15409) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 380, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864) [M::mem_pestat] mean and std.dev: (397.18, 138.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1051) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (659, 1717, 4345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11717) [M::mem_pestat] mean and std.dev: (2767.97, 2656.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15403) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (650, 1621, 4019) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10757) [M::mem_pestat] mean and std.dev: (2665.87, 2569.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14126) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 267.009 CPU sec, 17.008 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3432, 91961, 3218, 3459) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (681, 1738, 4355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11703) [M::mem_pestat] mean and std.dev: (2824.35, 2688.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15377) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (304, 381, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 862) [M::mem_pestat] mean and std.dev: (398.20, 137.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1048) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (587, 1542, 3930) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10616) [M::mem_pestat] mean and std.dev: (2627.64, 2631.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13959) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (633, 1672, 4131) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11127) [M::mem_pestat] mean and std.dev: (2707.46, 2617.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14625) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 268.641 CPU sec, 17.103 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3416, 91719, 3219, 3494) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (660, 1611, 4059) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10857) [M::mem_pestat] mean and std.dev: (2674.55, 2578.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14256) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 381, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861) [M::mem_pestat] mean and std.dev: (397.32, 137.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1047) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (613, 1658, 4148) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11218) [M::mem_pestat] mean and std.dev: (2674.63, 2579.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14753) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (621, 1593, 4172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11274) [M::mem_pestat] mean and std.dev: (2699.73, 2631.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14825) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 268.120 CPU sec, 17.163 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3565, 91865, 3213, 3418) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (640, 1620, 4210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11350) [M::mem_pestat] mean and std.dev: (2657.54, 2558.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14920) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 381, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 866) [M::mem_pestat] mean and std.dev: (397.14, 138.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1054) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (630, 1740, 4386) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11898) [M::mem_pestat] mean and std.dev: (2793.98, 2684.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15654) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (672, 1726, 4316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11604) [M::mem_pestat] mean and std.dev: (2778.29, 2642.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15248) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 267.295 CPU sec, 16.948 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3519, 91538, 3209, 3417) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (643, 1637, 4084) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10966) [M::mem_pestat] mean and std.dev: (2714.30, 2616.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14407) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 379, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 866) [M::mem_pestat] mean and std.dev: (396.83, 138.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1054) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (579, 1596, 3963) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10731) [M::mem_pestat] mean and std.dev: (2640.86, 2597.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14115) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (656, 1679, 4030) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10778) [M::mem_pestat] mean and std.dev: (2695.62, 2628.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14152) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 270.358 CPU sec, 17.534 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3410, 92094, 3253, 3406) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (644, 1570, 4101) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11015) [M::mem_pestat] mean and std.dev: (2662.45, 2584.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14472) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 380, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864) [M::mem_pestat] mean and std.dev: (397.46, 138.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1051) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (595, 1626, 4126) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11188) [M::mem_pestat] mean and std.dev: (2694.70, 2654.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14719) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (663, 1669, 3977) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10605) [M::mem_pestat] mean and std.dev: (2632.30, 2540.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13919) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 269.336 CPU sec, 17.265 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3446, 91806, 3199, 3313) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (618, 1609, 3925) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10539) [M::mem_pestat] mean and std.dev: (2614.71, 2538.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13846) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (304, 380, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 856) [M::mem_pestat] mean and std.dev: (396.68, 136.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1040) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (638, 1759, 4258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11498) [M::mem_pestat] mean and std.dev: (2751.05, 2635.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15118) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (635, 1589, 4002) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10736) [M::mem_pestat] mean and std.dev: (2652.62, 2610.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14103) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 268.088 CPU sec, 17.053 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3515, 91569, 3324, 3363) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (664, 1627, 4075) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10897) [M::mem_pestat] mean and std.dev: (2683.19, 2575.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14308) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 380, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864) [M::mem_pestat] mean and std.dev: (397.36, 138.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1051) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (665, 1744, 4492) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12146) [M::mem_pestat] mean and std.dev: (2836.17, 2695.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15973) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (671, 1630, 4231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11351) [M::mem_pestat] mean and std.dev: (2712.15, 2597.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14911) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 269.807 CPU sec, 17.243 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3510, 91397, 3372, 3421) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (651, 1579, 4020) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10758) [M::mem_pestat] mean and std.dev: (2647.83, 2559.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14127) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 379, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860) [M::mem_pestat] mean and std.dev: (395.95, 136.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1046) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (661, 1728, 4165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11173) [M::mem_pestat] mean and std.dev: (2709.04, 2573.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14677) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (650, 1598, 4066) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10898) [M::mem_pestat] mean and std.dev: (2650.97, 2543.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14314) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 265.977 CPU sec, 16.947 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3447, 91640, 3302, 3379) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (649, 1698, 4062) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10888) [M::mem_pestat] mean and std.dev: (2696.97, 2582.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14301) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 380, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861) [M::mem_pestat] mean and std.dev: (397.23, 137.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1047) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (613, 1730, 4255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11539) [M::mem_pestat] mean and std.dev: (2727.70, 2607.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15181) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (661, 1697, 4111) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11011) [M::mem_pestat] mean and std.dev: (2746.49, 2650.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14461) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 269.487 CPU sec, 17.151 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3412, 91698, 3255, 3409) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (641, 1662, 3928) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10502) [M::mem_pestat] mean and std.dev: (2626.09, 2531.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13789) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (301, 378, 487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 859) [M::mem_pestat] mean and std.dev: (395.03, 137.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1045) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (617, 1614, 4394) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11948) [M::mem_pestat] mean and std.dev: (2756.96, 2710.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15725) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (660, 1672, 4210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11310) [M::mem_pestat] mean and std.dev: (2730.37, 2611.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14860) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 267.703 CPU sec, 17.217 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3436, 92705, 3375, 3498) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (664, 1704, 4095) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10957) [M::mem_pestat] mean and std.dev: (2703.13, 2584.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14388) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (301, 378, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 868) [M::mem_pestat] mean and std.dev: (396.76, 139.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1057) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (595, 1646, 3792) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10186) [M::mem_pestat] mean and std.dev: (2596.19, 2540.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13383) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (666, 1602, 4199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11265) [M::mem_pestat] mean and std.dev: (2665.10, 2550.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14798) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 262.107 CPU sec, 16.669 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3280, 92737, 3260, 3470) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (629, 1572, 4077) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10973) [M::mem_pestat] mean and std.dev: (2629.08, 2548.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14421) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (301, 379, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 865) [M::mem_pestat] mean and std.dev: (396.14, 137.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1053) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (581, 1643, 4034) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10940) [M::mem_pestat] mean and std.dev: (2642.34, 2585.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14393) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (637, 1602, 4254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11488) [M::mem_pestat] mean and std.dev: (2751.21, 2677.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15105) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 261.640 CPU sec, 16.631 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3445, 92203, 3475, 3444) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (646, 1737, 4373) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11827) [M::mem_pestat] mean and std.dev: (2822.24, 2711.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15554) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 380, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861) [M::mem_pestat] mean and std.dev: (396.77, 137.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1047) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (568, 1553, 4005) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10879) [M::mem_pestat] mean and std.dev: (2603.97, 2614.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14316) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (614, 1557, 4138) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11186) [M::mem_pestat] mean and std.dev: (2686.00, 2606.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14710) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 260.866 CPU sec, 16.789 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3367, 92144, 3212, 3393) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (659, 1655, 4004) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10694) [M::mem_pestat] mean and std.dev: (2653.96, 2521.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14039) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (301, 380, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 865) [M::mem_pestat] mean and std.dev: (396.47, 138.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1053) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (633, 1749, 4250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11484) [M::mem_pestat] mean and std.dev: (2722.57, 2596.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15101) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (643, 1620, 4057) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10885) [M::mem_pestat] mean and std.dev: (2708.83, 2649.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14299) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 264.111 CPU sec, 16.755 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3479, 91751, 3253, 3457) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (654, 1700, 4158) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11166) [M::mem_pestat] mean and std.dev: (2747.21, 2635.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14670) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 379, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860) [M::mem_pestat] mean and std.dev: (395.51, 137.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1046) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (620, 1749, 4162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11246) [M::mem_pestat] mean and std.dev: (2732.27, 2640.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14788) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (621, 1588, 4041) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10881) [M::mem_pestat] mean and std.dev: (2669.63, 2614.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14301) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 263.984 CPU sec, 16.907 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3385, 92164, 3263, 3467) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (638, 1687, 4189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11291) [M::mem_pestat] mean and std.dev: (2733.93, 2619.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14842) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 380, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 863) [M::mem_pestat] mean and std.dev: (397.32, 138.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1050) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (619, 1717, 3947) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10603) [M::mem_pestat] mean and std.dev: (2642.53, 2555.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13931) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (651, 1676, 4179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11235) [M::mem_pestat] mean and std.dev: (2729.89, 2629.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14763) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 262.617 CPU sec, 16.657 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3457, 92169, 3112, 3343) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (621, 1582, 3887) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10419) [M::mem_pestat] mean and std.dev: (2634.01, 2564.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13685) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 381, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 867) [M::mem_pestat] mean and std.dev: (398.27, 138.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1055) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (614, 1576, 4191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11345) [M::mem_pestat] mean and std.dev: (2716.98, 2686.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14922) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (656, 1797, 4347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11729) [M::mem_pestat] mean and std.dev: (2780.42, 2631.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15420) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 264.793 CPU sec, 16.919 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3508, 92013, 3330, 3385) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (604, 1540, 3979) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10729) [M::mem_pestat] mean and std.dev: (2611.61, 2573.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14104) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 380, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 867) [M::mem_pestat] mean and std.dev: (397.77, 138.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1055) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (609, 1632, 4078) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11016) [M::mem_pestat] mean and std.dev: (2660.04, 2595.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14485) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (650, 1680, 4358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11774) [M::mem_pestat] mean and std.dev: (2755.46, 2647.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15482) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 263.085 CPU sec, 16.682 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3476, 91685, 3329, 3380) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (650, 1653, 4100) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11000) [M::mem_pestat] mean and std.dev: (2719.25, 2583.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14450) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 380, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 867) [M::mem_pestat] mean and std.dev: (397.62, 138.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1055) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (600, 1579, 4063) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10989) [M::mem_pestat] mean and std.dev: (2670.90, 2661.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14452) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (664, 1776, 4114) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11014) [M::mem_pestat] mean and std.dev: (2734.67, 2596.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14464) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 264.521 CPU sec, 16.906 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3445, 92213, 3341, 3468) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (663, 1725, 4232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11370) [M::mem_pestat] mean and std.dev: (2768.09, 2648.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14939) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 380, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864) [M::mem_pestat] mean and std.dev: (397.72, 139.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1051) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (602, 1636, 4204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11408) [M::mem_pestat] mean and std.dev: (2671.46, 2604.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15010) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (666, 1752, 4281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11511) [M::mem_pestat] mean and std.dev: (2818.58, 2684.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15126) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 260.859 CPU sec, 16.537 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3402, 91199, 3374, 3507) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (643, 1660, 4127) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11095) [M::mem_pestat] mean and std.dev: (2713.46, 2618.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14579) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 381, 494) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 876) [M::mem_pestat] mean and std.dev: (399.32, 140.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1067) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (619, 1685, 4159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11239) [M::mem_pestat] mean and std.dev: (2690.59, 2602.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14779) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (638, 1557, 3959) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10601) [M::mem_pestat] mean and std.dev: (2636.32, 2598.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13922) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 260.236 CPU sec, 16.699 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3411, 91542, 3255, 3420) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (647, 1558, 3793) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10085) [M::mem_pestat] mean and std.dev: (2593.31, 2546.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13231) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (304, 382, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 865) [M::mem_pestat] mean and std.dev: (398.66, 138.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1052) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (670, 1696, 4402) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11866) [M::mem_pestat] mean and std.dev: (2784.69, 2684.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15598) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (615, 1513, 3915) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10515) [M::mem_pestat] mean and std.dev: (2617.39, 2604.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13815) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 260.773 CPU sec, 16.555 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3289, 90432, 3321, 3610) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (653, 1699, 4063) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10883) [M::mem_pestat] mean and std.dev: (2734.43, 2623.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14293) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 381, 492) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 870) [M::mem_pestat] mean and std.dev: (398.60, 140.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1059) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (645, 1824, 4293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11589) [M::mem_pestat] mean and std.dev: (2793.88, 2637.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15237) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (636, 1573, 3929) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10515) [M::mem_pestat] mean and std.dev: (2627.68, 2554.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13808) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 263.038 CPU sec, 16.765 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3484, 92086, 3342, 3453) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (615, 1648, 3879) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10407) [M::mem_pestat] mean and std.dev: (2645.55, 2589.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13671) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 381, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 867) [M::mem_pestat] mean and std.dev: (398.17, 138.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1055) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (627, 1629, 4074) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10968) [M::mem_pestat] mean and std.dev: (2661.54, 2602.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14415) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (640, 1537, 4039) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10837) [M::mem_pestat] mean and std.dev: (2679.95, 2625.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14236) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 264.202 CPU sec, 16.902 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3378, 91238, 3218, 3499) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (671, 1624, 3978) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10592) [M::mem_pestat] mean and std.dev: (2655.63, 2568.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13899) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (304, 383, 494) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 874) [M::mem_pestat] mean and std.dev: (400.27, 139.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1064) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (630, 1726, 4091) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11013) [M::mem_pestat] mean and std.dev: (2736.75, 2664.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14474) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (648, 1669, 4014) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10746) [M::mem_pestat] mean and std.dev: (2673.82, 2584.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14112) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 262.466 CPU sec, 17.108 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3433, 91613, 3341, 3413) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (651, 1582, 4058) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10872) [M::mem_pestat] mean and std.dev: (2674.72, 2606.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14279) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (304, 382, 493) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 871) [M::mem_pestat] mean and std.dev: (400.23, 140.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1060) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (617, 1696, 4202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11372) [M::mem_pestat] mean and std.dev: (2708.09, 2635.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14957) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (652, 1719, 4255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11461) [M::mem_pestat] mean and std.dev: (2742.93, 2598.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15064) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 264.458 CPU sec, 16.808 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3398, 91457, 3264, 3667) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (613, 1657, 4066) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10972) [M::mem_pestat] mean and std.dev: (2704.00, 2620.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14425) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (304, 382, 492) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 868) [M::mem_pestat] mean and std.dev: (399.46, 139.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1056) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (639, 1757, 4314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11664) [M::mem_pestat] mean and std.dev: (2782.94, 2667.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15339) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (690, 1718, 4093) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10899) [M::mem_pestat] mean and std.dev: (2732.86, 2600.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14302) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 264.593 CPU sec, 16.849 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3444, 91840, 3267, 3581) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (631, 1620, 4067) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10939) [M::mem_pestat] mean and std.dev: (2630.67, 2507.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14375) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (305, 383, 492) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 866) [M::mem_pestat] mean and std.dev: (399.87, 138.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1053) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (583, 1585, 3971) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10747) [M::mem_pestat] mean and std.dev: (2632.92, 2598.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14135) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (645, 1637, 4113) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11049) [M::mem_pestat] mean and std.dev: (2702.33, 2615.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14517) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 266.825 CPU sec, 16.969 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3370, 91384, 3407, 3601) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (643, 1576, 4005) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10729) [M::mem_pestat] mean and std.dev: (2654.09, 2580.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14091) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 383, 495) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 879) [M::mem_pestat] mean and std.dev: (400.08, 141.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1071) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (579, 1568, 4151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11295) [M::mem_pestat] mean and std.dev: (2665.83, 2666.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14867) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (651, 1639, 4129) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11085) [M::mem_pestat] mean and std.dev: (2715.72, 2575.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14563) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 263.157 CPU sec, 16.763 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3463, 91615, 3365, 3396) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (613, 1663, 4033) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10873) [M::mem_pestat] mean and std.dev: (2676.18, 2564.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14293) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (304, 382, 493) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 871) [M::mem_pestat] mean and std.dev: (399.29, 138.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1060) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (649, 1726, 4181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11245) [M::mem_pestat] mean and std.dev: (2759.99, 2677.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14777) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (604, 1560, 3905) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10507) [M::mem_pestat] mean and std.dev: (2619.67, 2589.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13808) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 265.882 CPU sec, 17.084 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3335, 91140, 3298, 3405) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (663, 1679, 4195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11259) [M::mem_pestat] mean and std.dev: (2752.50, 2638.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14791) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (304, 383, 493) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 871) [M::mem_pestat] mean and std.dev: (399.90, 139.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1060) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (598, 1697, 4200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11404) [M::mem_pestat] mean and std.dev: (2729.02, 2667.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15006) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (630, 1612, 4045) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10875) [M::mem_pestat] mean and std.dev: (2651.01, 2569.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14290) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 266.184 CPU sec, 16.962 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3503, 91387, 3307, 3369) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (641, 1591, 4077) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10949) [M::mem_pestat] mean and std.dev: (2666.86, 2575.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14385) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (304, 383, 494) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 874) [M::mem_pestat] mean and std.dev: (400.08, 140.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1064) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (649, 1696, 4214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11344) [M::mem_pestat] mean and std.dev: (2732.02, 2632.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14909) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (630, 1694, 4267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11541) [M::mem_pestat] mean and std.dev: (2724.28, 2594.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15178) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 267.204 CPU sec, 16.956 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3508, 91141, 3258, 3398) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (688, 1768, 4234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11326) [M::mem_pestat] mean and std.dev: (2785.14, 2637.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14872) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (304, 382, 492) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 868) [M::mem_pestat] mean and std.dev: (399.43, 139.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1056) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (643, 1869, 4438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12028) [M::mem_pestat] mean and std.dev: (2849.04, 2692.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15823) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (616, 1665, 4018) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10822) [M::mem_pestat] mean and std.dev: (2647.24, 2562.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14224) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 264.910 CPU sec, 16.943 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3554, 90950, 3379, 3431) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (689, 1647, 4201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11225) [M::mem_pestat] mean and std.dev: (2748.48, 2646.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14737) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (304, 382, 492) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 868) [M::mem_pestat] mean and std.dev: (399.26, 139.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1056) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (645, 1764, 4286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11568) [M::mem_pestat] mean and std.dev: (2756.91, 2600.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15209) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (626, 1616, 4059) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10925) [M::mem_pestat] mean and std.dev: (2655.61, 2593.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14358) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 263.752 CPU sec, 16.765 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3411, 91684, 3311, 3435) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (651, 1616, 4359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11775) [M::mem_pestat] mean and std.dev: (2758.21, 2657.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15483) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 383, 492) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 870) [M::mem_pestat] mean and std.dev: (398.98, 139.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1059) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (627, 1661, 4192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11322) [M::mem_pestat] mean and std.dev: (2726.78, 2650.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14887) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (627, 1634, 4075) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10971) [M::mem_pestat] mean and std.dev: (2657.01, 2558.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14419) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 265.363 CPU sec, 17.095 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3495, 91572, 3320, 3444) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (663, 1616, 3996) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10662) [M::mem_pestat] mean and std.dev: (2699.55, 2616.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13995) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (304, 382, 492) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 868) [M::mem_pestat] mean and std.dev: (399.56, 139.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1056) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (631, 1621, 4115) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11083) [M::mem_pestat] mean and std.dev: (2692.76, 2640.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14567) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (671, 1693, 3988) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10622) [M::mem_pestat] mean and std.dev: (2666.26, 2536.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13939) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 265.081 CPU sec, 16.843 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3470, 91321, 3328, 3405) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (688, 1806, 4300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11524) [M::mem_pestat] mean and std.dev: (2807.50, 2624.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15136) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (304, 382, 492) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 868) [M::mem_pestat] mean and std.dev: (399.87, 139.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1056) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (619, 1679, 4135) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11167) [M::mem_pestat] mean and std.dev: (2726.95, 2648.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14683) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (685, 1715, 4191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11203) [M::mem_pestat] mean and std.dev: (2737.01, 2606.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14709) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 264.368 CPU sec, 16.935 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3358, 91317, 3416, 3385) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (651, 1640, 3996) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10686) [M::mem_pestat] mean and std.dev: (2656.91, 2578.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14031) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 382, 493) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 873) [M::mem_pestat] mean and std.dev: (399.05, 139.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1063) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (647, 1698, 4482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12152) [M::mem_pestat] mean and std.dev: (2796.20, 2671.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15987) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (651, 1675, 4237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11409) [M::mem_pestat] mean and std.dev: (2763.87, 2627.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14995) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 266.155 CPU sec, 16.872 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3396, 91279, 3268, 3387) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (661, 1679, 4310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11608) [M::mem_pestat] mean and std.dev: (2761.14, 2622.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15257) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] (25, 50, 75) percentile: (304, 381, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 865) [M::mem_pestat] mean and std.dev: (398.55, 138.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1052) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (608, 1653, 4274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11606) [M::mem_pestat] mean and std.dev: (2725.67, 2652.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15272) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (659, 1561, 4064) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10874) [M::mem_pestat] mean and std.dev: (2653.51, 2585.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14279) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 266.854 CPU sec, 17.005 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3420, 91299, 3394, 3499) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (661, 1663, 4025) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10753) [M::mem_pestat] mean and std.dev: (2661.14, 2558.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14117) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (304, 382, 493) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 871) [M::mem_pestat] mean and std.dev: (399.80, 139.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1060) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (632, 1644, 4204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11348) [M::mem_pestat] mean and std.dev: (2684.79, 2603.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14920) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (649, 1634, 4017) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10753) [M::mem_pestat] mean and std.dev: (2674.33, 2589.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14121) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 262.815 CPU sec, 16.699 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3637, 91218, 3507, 3474) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] (25, 50, 75) percentile: (659, 1645, 4169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11189) [M::mem_pestat] mean and std.dev: (2698.10, 2598.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14699) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (304, 381, 492) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 868) [M::mem_pestat] mean and std.dev: (399.15, 138.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1056) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (628, 1766, 4406) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11962) [M::mem_pestat] mean and std.dev: (2755.91, 2628.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15740) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (634, 1600, 4015) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10777) [M::mem_pestat] mean and std.dev: (2672.16, 2584.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14158) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 268.312 CPU sec, 17.363 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3470, 91692, 3323, 3415) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (617, 1568, 3816) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10214) [M::mem_pestat] mean and std.dev: (2578.17, 2518.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13413) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (304, 381, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 862) [M::mem_pestat] mean and std.dev: (398.12, 138.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1048) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (641, 1732, 4389) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11885) [M::mem_pestat] mean and std.dev: (2773.10, 2653.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15633) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (621, 1671, 4091) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11031) [M::mem_pestat] mean and std.dev: (2691.99, 2593.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14501) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 267.229 CPU sec, 17.003 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3331, 91112, 3293, 3399) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (651, 1604, 4002) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10704) [M::mem_pestat] mean and std.dev: (2662.55, 2561.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14055) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (304, 382, 492) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 868) [M::mem_pestat] mean and std.dev: (399.05, 139.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1056) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (639, 1705, 4418) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11976) [M::mem_pestat] mean and std.dev: (2786.07, 2690.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15755) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (665, 1831, 4310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11600) [M::mem_pestat] mean and std.dev: (2787.51, 2615.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15245) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 266.026 CPU sec, 16.981 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3428, 91412, 3328, 3429) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (661, 1730, 4134) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11080) [M::mem_pestat] mean and std.dev: (2764.41, 2633.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14553) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (304, 382, 494) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 874) [M::mem_pestat] mean and std.dev: (399.71, 139.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1064) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (621, 1662, 4220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11418) [M::mem_pestat] mean and std.dev: (2716.05, 2638.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15017) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (635, 1697, 4216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11378) [M::mem_pestat] mean and std.dev: (2720.38, 2596.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14959) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 267.861 CPU sec, 17.178 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3436, 90934, 3225, 3456) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (686, 1780, 4281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11471) [M::mem_pestat] mean and std.dev: (2754.15, 2581.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15066) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 381, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 867) [M::mem_pestat] mean and std.dev: (398.33, 138.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1055) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (621, 1701, 4249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11505) [M::mem_pestat] mean and std.dev: (2736.60, 2665.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15133) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (682, 1623, 4049) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10783) [M::mem_pestat] mean and std.dev: (2676.73, 2578.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14150) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 266.781 CPU sec, 16.953 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3587, 91964, 3322, 3395) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (605, 1544, 3886) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10448) [M::mem_pestat] mean and std.dev: (2578.67, 2559.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13729) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (304, 382, 493) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 871) [M::mem_pestat] mean and std.dev: (399.12, 138.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1060) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (644, 1661, 4067) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10913) [M::mem_pestat] mean and std.dev: (2702.35, 2627.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14336) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (656, 1635, 3994) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10670) [M::mem_pestat] mean and std.dev: (2677.37, 2580.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14008) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 264.781 CPU sec, 17.030 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3416, 91320, 3299, 3385) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (661, 1664, 3976) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10606) [M::mem_pestat] mean and std.dev: (2667.16, 2557.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13921) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 382, 492) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 870) [M::mem_pestat] mean and std.dev: (399.26, 139.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1059) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (591, 1660, 4174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11340) [M::mem_pestat] mean and std.dev: (2717.76, 2684.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14923) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (647, 1616, 3917) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10457) [M::mem_pestat] mean and std.dev: (2628.20, 2561.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13727) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 268.008 CPU sec, 17.037 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3443, 91127, 3328, 3358) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (639, 1676, 4356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11790) [M::mem_pestat] mean and std.dev: (2784.87, 2680.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15507) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 382, 493) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 873) [M::mem_pestat] mean and std.dev: (399.58, 140.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1063) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (610, 1606, 4032) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10876) [M::mem_pestat] mean and std.dev: (2619.95, 2574.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14298) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (625, 1589, 3894) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10432) [M::mem_pestat] mean and std.dev: (2593.12, 2517.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13701) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 264.475 CPU sec, 16.813 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3369, 91637, 3287, 3423) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (687, 1640, 4070) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10836) [M::mem_pestat] mean and std.dev: (2684.02, 2553.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14219) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 382, 492) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 870) [M::mem_pestat] mean and std.dev: (398.68, 138.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1059) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (617, 1666, 4142) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11192) [M::mem_pestat] mean and std.dev: (2710.77, 2618.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14717) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (643, 1719, 4065) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10909) [M::mem_pestat] mean and std.dev: (2658.60, 2529.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14331) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 267.663 CPU sec, 17.329 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3365, 91325, 3369, 3486) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (665, 1683, 4377) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11801) [M::mem_pestat] mean and std.dev: (2793.19, 2651.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15513) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 381, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 867) [M::mem_pestat] mean and std.dev: (398.31, 138.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1055) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (606, 1761, 4335) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11793) [M::mem_pestat] mean and std.dev: (2790.88, 2674.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15522) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (616, 1624, 4046) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10906) [M::mem_pestat] mean and std.dev: (2665.94, 2613.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14336) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 269.217 CPU sec, 17.118 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3478, 92164, 3320, 3434) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (630, 1656, 3984) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10692) [M::mem_pestat] mean and std.dev: (2661.30, 2576.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14046) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (304, 382, 492) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 868) [M::mem_pestat] mean and std.dev: (398.77, 138.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1056) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (612, 1552, 4042) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10902) [M::mem_pestat] mean and std.dev: (2657.14, 2634.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14332) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (655, 1768, 4228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11374) [M::mem_pestat] mean and std.dev: (2765.39, 2592.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14947) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 267.554 CPU sec, 17.088 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3365, 91704, 3350, 3522) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (623, 1681, 4135) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11159) [M::mem_pestat] mean and std.dev: (2703.59, 2621.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14671) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 381, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 869) [M::mem_pestat] mean and std.dev: (398.20, 139.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1058) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (629, 1647, 4161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11225) [M::mem_pestat] mean and std.dev: (2710.67, 2610.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14757) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (603, 1612, 4117) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11145) [M::mem_pestat] mean and std.dev: (2700.85, 2644.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14659) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 266.846 CPU sec, 16.924 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3341, 91180, 3359, 3347) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (627, 1527, 3908) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10470) [M::mem_pestat] mean and std.dev: (2638.48, 2609.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13751) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 381, 492) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 870) [M::mem_pestat] mean and std.dev: (398.55, 139.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1059) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (659, 1869, 4461) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12065) [M::mem_pestat] mean and std.dev: (2832.64, 2651.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15867) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (627, 1578, 3851) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10299) [M::mem_pestat] mean and std.dev: (2596.73, 2553.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13523) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 266.844 CPU sec, 17.176 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3449, 91277, 3219, 3466) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (618, 1591, 4093) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11043) [M::mem_pestat] mean and std.dev: (2657.27, 2596.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14518) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 380, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 869) [M::mem_pestat] mean and std.dev: (397.93, 139.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1058) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (623, 1768, 4233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11453) [M::mem_pestat] mean and std.dev: (2752.64, 2623.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15063) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (675, 1718, 4300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11550) [M::mem_pestat] mean and std.dev: (2760.33, 2610.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15175) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 266.101 CPU sec, 16.912 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3403, 91752, 3162, 3363) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (667, 1640, 4255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11431) [M::mem_pestat] mean and std.dev: (2760.90, 2652.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15019) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 380, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864) [M::mem_pestat] mean and std.dev: (397.56, 138.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1051) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (617, 1689, 4427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12047) [M::mem_pestat] mean and std.dev: (2773.32, 2668.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15857) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (668, 1753, 4119) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11021) [M::mem_pestat] mean and std.dev: (2759.03, 2631.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14472) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 266.140 CPU sec, 17.027 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3374, 91522, 3241, 3394) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (614, 1660, 4208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11396) [M::mem_pestat] mean and std.dev: (2728.88, 2638.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14990) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 381, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864) [M::mem_pestat] mean and std.dev: (397.71, 138.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1051) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (636, 1717, 4131) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11121) [M::mem_pestat] mean and std.dev: (2735.80, 2658.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14616) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (667, 1730, 4084) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10918) [M::mem_pestat] mean and std.dev: (2701.42, 2557.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14335) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 267.247 CPU sec, 16.956 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3387, 91577, 3455, 3296) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (680, 1671, 4108) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10964) [M::mem_pestat] mean and std.dev: (2706.21, 2596.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14392) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 381, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864) [M::mem_pestat] mean and std.dev: (397.05, 137.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1051) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (612, 1601, 3999) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10773) [M::mem_pestat] mean and std.dev: (2639.64, 2579.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14160) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (625, 1578, 3895) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10435) [M::mem_pestat] mean and std.dev: (2597.36, 2526.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13705) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 268.182 CPU sec, 17.145 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3432, 91696, 3255, 3482) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (652, 1621, 4034) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10798) [M::mem_pestat] mean and std.dev: (2646.54, 2541.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14180) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 380, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 867) [M::mem_pestat] mean and std.dev: (397.73, 138.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1055) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (621, 1629, 4046) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10896) [M::mem_pestat] mean and std.dev: (2671.99, 2590.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14321) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (672, 1645, 4219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11313) [M::mem_pestat] mean and std.dev: (2755.17, 2636.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14860) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 266.085 CPU sec, 16.906 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3363, 91350, 3224, 3388) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (639, 1719, 4421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11985) [M::mem_pestat] mean and std.dev: (2806.64, 2675.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15767) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 380, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 867) [M::mem_pestat] mean and std.dev: (397.77, 138.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1055) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (617, 1606, 4169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11273) [M::mem_pestat] mean and std.dev: (2718.85, 2663.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14825) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (628, 1620, 4128) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11128) [M::mem_pestat] mean and std.dev: (2676.85, 2588.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14628) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 268.400 CPU sec, 17.333 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3390, 91875, 3216, 3596) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (663, 1606, 4103) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10983) [M::mem_pestat] mean and std.dev: (2708.01, 2635.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14423) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 381, 492) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 870) [M::mem_pestat] mean and std.dev: (398.50, 139.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1059) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (597, 1681, 4085) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11061) [M::mem_pestat] mean and std.dev: (2668.46, 2590.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14549) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (661, 1651, 4111) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11011) [M::mem_pestat] mean and std.dev: (2711.20, 2620.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14461) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 267.402 CPU sec, 17.002 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3355, 92049, 3210, 3425) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (680, 1800, 4333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11639) [M::mem_pestat] mean and std.dev: (2787.13, 2604.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15292) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 379, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 863) [M::mem_pestat] mean and std.dev: (396.80, 138.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1050) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (612, 1666, 4070) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10986) [M::mem_pestat] mean and std.dev: (2661.16, 2570.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14444) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (712, 1826, 4328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11560) [M::mem_pestat] mean and std.dev: (2794.84, 2611.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15176) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 268.189 CPU sec, 17.065 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3421, 91042, 3335, 3393) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (660, 1701, 4220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11340) [M::mem_pestat] mean and std.dev: (2739.80, 2591.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14900) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 380, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 866) [M::mem_pestat] mean and std.dev: (397.64, 139.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1054) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (615, 1757, 4420) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12030) [M::mem_pestat] mean and std.dev: (2821.98, 2710.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15835) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (657, 1653, 4327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11667) [M::mem_pestat] mean and std.dev: (2757.21, 2665.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15337) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 269.648 CPU sec, 17.244 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3424, 91997, 3328, 3434) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (692, 1707, 4260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11396) [M::mem_pestat] mean and std.dev: (2751.12, 2598.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14964) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 380, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860) [M::mem_pestat] mean and std.dev: (397.04, 138.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1046) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (628, 1726, 4140) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11164) [M::mem_pestat] mean and std.dev: (2706.62, 2601.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14676) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (645, 1615, 4112) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11046) [M::mem_pestat] mean and std.dev: (2711.49, 2628.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14513) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 267.457 CPU sec, 16.944 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3332, 91583, 3479, 3350) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (653, 1629, 4175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11219) [M::mem_pestat] mean and std.dev: (2695.90, 2600.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14741) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 381, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861) [M::mem_pestat] mean and std.dev: (396.88, 138.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1047) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (621, 1620, 4294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11640) [M::mem_pestat] mean and std.dev: (2749.09, 2684.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15313) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (657, 1658, 4207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11307) [M::mem_pestat] mean and std.dev: (2712.60, 2582.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14857) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 270.877 CPU sec, 17.301 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3495, 91741, 3311, 3441) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (657, 1650, 4251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11439) [M::mem_pestat] mean and std.dev: (2756.84, 2666.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15033) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 380, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864) [M::mem_pestat] mean and std.dev: (397.06, 138.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1051) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (611, 1613, 3861) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10361) [M::mem_pestat] mean and std.dev: (2631.35, 2591.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13611) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (692, 1731, 4057) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10787) [M::mem_pestat] mean and std.dev: (2746.57, 2622.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14152) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 271.477 CPU sec, 17.286 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3410, 91472, 3369, 3304) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (648, 1709, 4113) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11043) [M::mem_pestat] mean and std.dev: (2710.71, 2600.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14508) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 380, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 863) [M::mem_pestat] mean and std.dev: (396.68, 138.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1050) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (597, 1639, 4098) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11100) [M::mem_pestat] mean and std.dev: (2663.16, 2607.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14601) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (657, 1674, 3946) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10524) [M::mem_pestat] mean and std.dev: (2645.85, 2536.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13813) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 271.196 CPU sec, 17.760 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3415, 91376, 3298, 3531) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (639, 1676, 4154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11184) [M::mem_pestat] mean and std.dev: (2741.13, 2640.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14699) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 380, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864) [M::mem_pestat] mean and std.dev: (398.09, 138.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1051) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (644, 1703, 4179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11249) [M::mem_pestat] mean and std.dev: (2694.50, 2564.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14784) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (630, 1528, 3991) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10713) [M::mem_pestat] mean and std.dev: (2638.66, 2608.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14074) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 270.368 CPU sec, 17.349 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3327, 91719, 3278, 3401) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (626, 1598, 4115) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11093) [M::mem_pestat] mean and std.dev: (2674.97, 2602.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14582) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 381, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 867) [M::mem_pestat] mean and std.dev: (398.09, 139.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1055) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (593, 1677, 4229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11501) [M::mem_pestat] mean and std.dev: (2705.96, 2636.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15137) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (673, 1715, 4287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11515) [M::mem_pestat] mean and std.dev: (2787.94, 2644.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15129) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 267.744 CPU sec, 17.038 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3362, 91450, 3249, 3409) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (679, 1719, 4188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11206) [M::mem_pestat] mean and std.dev: (2759.81, 2631.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14715) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 380, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860) [M::mem_pestat] mean and std.dev: (396.81, 137.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1046) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (608, 1712, 3989) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10751) [M::mem_pestat] mean and std.dev: (2669.33, 2581.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14132) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (649, 1761, 4124) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11074) [M::mem_pestat] mean and std.dev: (2772.87, 2627.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14549) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 267.685 CPU sec, 17.255 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3614, 91860, 3286, 3391) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (662, 1675, 4186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11234) [M::mem_pestat] mean and std.dev: (2719.18, 2610.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14758) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 380, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 869) [M::mem_pestat] mean and std.dev: (397.45, 138.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1058) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (596, 1636, 4171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11321) [M::mem_pestat] mean and std.dev: (2719.02, 2688.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14896) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (675, 1634, 4193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11229) [M::mem_pestat] mean and std.dev: (2721.22, 2614.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14747) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 271.497 CPU sec, 17.254 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3396, 92086, 3365, 3448) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (648, 1690, 4227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11385) [M::mem_pestat] mean and std.dev: (2758.72, 2651.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14964) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 380, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864) [M::mem_pestat] mean and std.dev: (397.67, 138.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1051) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (606, 1651, 4072) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11004) [M::mem_pestat] mean and std.dev: (2698.16, 2622.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14470) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (644, 1632, 3968) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10616) [M::mem_pestat] mean and std.dev: (2645.38, 2541.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13940) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 266.267 CPU sec, 17.129 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3444, 92680, 3297, 3399) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (650, 1623, 3982) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10646) [M::mem_pestat] mean and std.dev: (2669.17, 2589.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13978) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 380, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 866) [M::mem_pestat] mean and std.dev: (396.99, 138.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1054) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (609, 1664, 4184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11334) [M::mem_pestat] mean and std.dev: (2678.11, 2610.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14909) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (677, 1713, 4235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11351) [M::mem_pestat] mean and std.dev: (2759.23, 2640.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14909) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 263.281 CPU sec, 16.703 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3381, 92797, 3292, 3574) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (624, 1608, 3881) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10395) [M::mem_pestat] mean and std.dev: (2640.59, 2565.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13652) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 379, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860) [M::mem_pestat] mean and std.dev: (395.72, 137.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1046) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (572, 1563, 4150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11306) [M::mem_pestat] mean and std.dev: (2651.48, 2623.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14884) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (611, 1529, 4110) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11108) [M::mem_pestat] mean and std.dev: (2636.42, 2598.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14607) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 263.871 CPU sec, 16.870 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3531, 92119, 3306, 3531) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (633, 1547, 3935) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10539) [M::mem_pestat] mean and std.dev: (2627.64, 2594.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13841) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 379, 487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 855) [M::mem_pestat] mean and std.dev: (395.12, 136.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1039) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (630, 1611, 4236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11448) [M::mem_pestat] mean and std.dev: (2688.90, 2595.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15054) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (618, 1641, 4284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11616) [M::mem_pestat] mean and std.dev: (2735.16, 2649.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15282) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 261.831 CPU sec, 16.732 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3514, 92500, 3305, 3388) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (640, 1550, 3832) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10216) [M::mem_pestat] mean and std.dev: (2623.22, 2580.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13408) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 379, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 866) [M::mem_pestat] mean and std.dev: (396.97, 138.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1054) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (602, 1608, 3923) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10565) [M::mem_pestat] mean and std.dev: (2598.82, 2535.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13886) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (612, 1714, 4115) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11121) [M::mem_pestat] mean and std.dev: (2692.61, 2609.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14624) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 261.140 CPU sec, 16.639 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3521, 92470, 3348, 3467) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (637, 1619, 4102) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11032) [M::mem_pestat] mean and std.dev: (2710.66, 2623.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14497) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 381, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 867) [M::mem_pestat] mean and std.dev: (398.32, 138.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1055) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (610, 1677, 4271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11593) [M::mem_pestat] mean and std.dev: (2796.77, 2735.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15254) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (641, 1597, 4110) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11048) [M::mem_pestat] mean and std.dev: (2684.77, 2607.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14517) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 262.678 CPU sec, 16.772 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3518, 91818, 3354, 3465) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (641, 1549, 4097) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11009) [M::mem_pestat] mean and std.dev: (2630.44, 2585.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14465) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 381, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 863) [M::mem_pestat] mean and std.dev: (396.93, 137.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1050) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (578, 1621, 3783) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10193) [M::mem_pestat] mean and std.dev: (2599.92, 2584.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13398) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (619, 1583, 4157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11233) [M::mem_pestat] mean and std.dev: (2698.84, 2661.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14771) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 264.136 CPU sec, 16.819 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3387, 91896, 3245, 3532) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (640, 1637, 4086) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10978) [M::mem_pestat] mean and std.dev: (2695.92, 2611.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14424) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (304, 380, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 862) [M::mem_pestat] mean and std.dev: (397.74, 138.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1048) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (610, 1588, 3875) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10405) [M::mem_pestat] mean and std.dev: (2596.99, 2553.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13670) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (659, 1676, 4132) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11078) [M::mem_pestat] mean and std.dev: (2729.30, 2622.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14551) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 260.971 CPU sec, 16.700 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3461, 91518, 3457, 3397) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (640, 1603, 4000) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10720) [M::mem_pestat] mean and std.dev: (2627.21, 2537.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14080) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 381, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 867) [M::mem_pestat] mean and std.dev: (398.51, 138.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1055) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (629, 1646, 4313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11681) [M::mem_pestat] mean and std.dev: (2769.02, 2683.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15365) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (675, 1694, 4200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11250) [M::mem_pestat] mean and std.dev: (2729.88, 2578.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14775) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 264.579 CPU sec, 16.809 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3393, 91893, 3357, 3481) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (637, 1666, 4094) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11008) [M::mem_pestat] mean and std.dev: (2706.57, 2619.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14465) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (304, 382, 493) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 871) [M::mem_pestat] mean and std.dev: (399.02, 139.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1060) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (625, 1736, 4197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11341) [M::mem_pestat] mean and std.dev: (2739.31, 2617.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14913) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (700, 1709, 4225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11275) [M::mem_pestat] mean and std.dev: (2758.75, 2604.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14800) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 262.331 CPU sec, 16.776 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3426, 91636, 3366, 3455) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (653, 1742, 4281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11537) [M::mem_pestat] mean and std.dev: (2758.68, 2628.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15165) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (304, 382, 493) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 871) [M::mem_pestat] mean and std.dev: (399.42, 139.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1060) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (662, 1786, 4462) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12062) [M::mem_pestat] mean and std.dev: (2849.10, 2714.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15862) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (646, 1595, 4136) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11116) [M::mem_pestat] mean and std.dev: (2667.58, 2563.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14606) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 259.242 CPU sec, 16.395 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3394, 91199, 3460, 3415) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (642, 1594, 4124) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11088) [M::mem_pestat] mean and std.dev: (2711.26, 2622.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14570) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 381, 492) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 870) [M::mem_pestat] mean and std.dev: (398.65, 138.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1059) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (568, 1633, 4230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11554) [M::mem_pestat] mean and std.dev: (2681.04, 2627.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15216) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (712, 1704, 4198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11170) [M::mem_pestat] mean and std.dev: (2769.60, 2637.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14656) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 263.749 CPU sec, 16.788 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3467, 91369, 3316, 3560) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (647, 1623, 4179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11243) [M::mem_pestat] mean and std.dev: (2737.70, 2648.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14775) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (304, 382, 493) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 871) [M::mem_pestat] mean and std.dev: (399.75, 139.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1060) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (650, 1693, 4129) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11087) [M::mem_pestat] mean and std.dev: (2727.30, 2621.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14566) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (624, 1618, 4136) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11160) [M::mem_pestat] mean and std.dev: (2699.88, 2603.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14672) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 262.489 CPU sec, 16.744 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3345, 91954, 3250, 3428) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (649, 1595, 3948) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10546) [M::mem_pestat] mean and std.dev: (2650.83, 2572.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13845) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (304, 383, 493) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 871) [M::mem_pestat] mean and std.dev: (399.36, 139.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1060) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (620, 1629, 4044) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10892) [M::mem_pestat] mean and std.dev: (2667.67, 2569.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14316) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (660, 1726, 4057) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10851) [M::mem_pestat] mean and std.dev: (2694.02, 2531.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14248) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 261.932 CPU sec, 16.681 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3418, 91803, 3280, 3315) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (672, 1738, 4035) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10761) [M::mem_pestat] mean and std.dev: (2713.06, 2603.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14124) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (304, 382, 493) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 871) [M::mem_pestat] mean and std.dev: (399.96, 139.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1060) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (625, 1576, 4209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11377) [M::mem_pestat] mean and std.dev: (2683.33, 2650.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14961) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (662, 1621, 3985) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10631) [M::mem_pestat] mean and std.dev: (2685.74, 2610.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13954) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 261.219 CPU sec, 16.604 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3503, 92060, 3230, 3528) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (674, 1616, 4032) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10748) [M::mem_pestat] mean and std.dev: (2686.67, 2579.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14106) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 382, 492) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 870) [M::mem_pestat] mean and std.dev: (398.79, 138.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1059) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (632, 1750, 4424) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12008) [M::mem_pestat] mean and std.dev: (2825.07, 2705.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15800) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (632, 1674, 3989) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10703) [M::mem_pestat] mean and std.dev: (2682.24, 2603.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14060) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 261.426 CPU sec, 16.778 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3383, 91574, 3311, 3418) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (658, 1664, 4247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11425) [M::mem_pestat] mean and std.dev: (2736.72, 2628.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15014) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (304, 382, 493) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 871) [M::mem_pestat] mean and std.dev: (399.40, 139.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1060) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (617, 1691, 4250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11516) [M::mem_pestat] mean and std.dev: (2697.83, 2608.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15149) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (641, 1626, 4046) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10856) [M::mem_pestat] mean and std.dev: (2708.04, 2645.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14261) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 263.748 CPU sec, 16.765 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3376, 91558, 3283, 3398) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (645, 1645, 4113) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11049) [M::mem_pestat] mean and std.dev: (2718.57, 2652.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14517) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 381, 492) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 870) [M::mem_pestat] mean and std.dev: (398.88, 139.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1059) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (600, 1606, 4076) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11028) [M::mem_pestat] mean and std.dev: (2652.46, 2583.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14504) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (628, 1611, 3948) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10588) [M::mem_pestat] mean and std.dev: (2633.23, 2570.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13908) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 262.320 CPU sec, 16.887 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3448, 91185, 3394, 3452) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (702, 1726, 4338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11610) [M::mem_pestat] mean and std.dev: (2781.96, 2616.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15246) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (304, 382, 492) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 868) [M::mem_pestat] mean and std.dev: (399.25, 139.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1056) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (667, 1809, 4302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11572) [M::mem_pestat] mean and std.dev: (2789.02, 2607.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15207) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (671, 1668, 4143) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11087) [M::mem_pestat] mean and std.dev: (2724.37, 2625.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14559) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 262.375 CPU sec, 16.646 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3572, 91619, 3376, 3528) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (674, 1721, 4218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11306) [M::mem_pestat] mean and std.dev: (2738.83, 2609.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14850) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 381, 492) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 870) [M::mem_pestat] mean and std.dev: (398.13, 139.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1059) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (651, 1720, 4174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11220) [M::mem_pestat] mean and std.dev: (2708.94, 2618.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14743) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (669, 1727, 4233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11361) [M::mem_pestat] mean and std.dev: (2770.91, 2630.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14925) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 262.865 CPU sec, 16.725 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3370, 91270, 3362, 3541) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (612, 1526, 3992) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10752) [M::mem_pestat] mean and std.dev: (2588.97, 2545.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14132) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (304, 382, 492) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 868) [M::mem_pestat] mean and std.dev: (398.82, 138.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1056) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (595, 1748, 4390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11980) [M::mem_pestat] mean and std.dev: (2768.44, 2684.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15775) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (648, 1664, 4180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11244) [M::mem_pestat] mean and std.dev: (2693.89, 2589.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14776) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 263.417 CPU sec, 16.743 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3465, 91019, 3385, 3406) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (662, 1717, 4309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11603) [M::mem_pestat] mean and std.dev: (2779.15, 2643.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15250) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (304, 383, 494) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 874) [M::mem_pestat] mean and std.dev: (400.16, 139.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1064) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (629, 1641, 4165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11237) [M::mem_pestat] mean and std.dev: (2700.86, 2616.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14773) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (659, 1634, 4121) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11045) [M::mem_pestat] mean and std.dev: (2721.34, 2641.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14507) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 262.209 CPU sec, 16.794 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3440, 91798, 3393, 3375) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (677, 1691, 4112) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10982) [M::mem_pestat] mean and std.dev: (2736.16, 2621.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14417) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (304, 383, 493) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 871) [M::mem_pestat] mean and std.dev: (399.64, 138.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1060) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (640, 1716, 4321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11683) [M::mem_pestat] mean and std.dev: (2761.45, 2645.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15364) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (627, 1714, 4211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11379) [M::mem_pestat] mean and std.dev: (2740.58, 2634.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14963) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 263.511 CPU sec, 16.742 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3386, 91663, 3376, 3419) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (667, 1640, 4218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11320) [M::mem_pestat] mean and std.dev: (2727.33, 2616.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14871) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (305, 382, 492) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 866) [M::mem_pestat] mean and std.dev: (399.05, 138.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1053) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (648, 1789, 4274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11526) [M::mem_pestat] mean and std.dev: (2770.24, 2646.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15152) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (669, 1728, 4262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11448) [M::mem_pestat] mean and std.dev: (2781.99, 2654.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15041) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 262.159 CPU sec, 16.885 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3291, 91629, 3307, 3376) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (680, 1718, 4159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11117) [M::mem_pestat] mean and std.dev: (2720.37, 2578.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14596) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (304, 383, 495) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 877) [M::mem_pestat] mean and std.dev: (401.22, 140.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1068) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (643, 1748, 4189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11281) [M::mem_pestat] mean and std.dev: (2750.50, 2651.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14827) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (640, 1671, 4219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11377) [M::mem_pestat] mean and std.dev: (2741.74, 2629.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14956) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 262.902 CPU sec, 16.676 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3548, 91220, 3322, 3515) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (630, 1626, 4252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11496) [M::mem_pestat] mean and std.dev: (2707.50, 2619.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15118) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (304, 381, 492) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 868) [M::mem_pestat] mean and std.dev: (398.97, 138.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1056) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (645, 1620, 4081) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10953) [M::mem_pestat] mean and std.dev: (2688.40, 2619.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14389) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (644, 1695, 4328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11696) [M::mem_pestat] mean and std.dev: (2771.82, 2631.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15380) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 266.320 CPU sec, 17.151 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3407, 92149, 3257, 3303) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (635, 1575, 3800) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10130) [M::mem_pestat] mean and std.dev: (2615.16, 2572.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13295) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (304, 381, 493) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 871) [M::mem_pestat] mean and std.dev: (398.84, 139.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1060) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (620, 1642, 4167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11261) [M::mem_pestat] mean and std.dev: (2692.06, 2627.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14808) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (679, 1688, 4206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11260) [M::mem_pestat] mean and std.dev: (2734.25, 2580.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14787) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 263.713 CPU sec, 16.720 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3469, 92225, 3268, 3489) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (668, 1775, 4380) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11804) [M::mem_pestat] mean and std.dev: (2815.18, 2670.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15516) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 381, 492) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 870) [M::mem_pestat] mean and std.dev: (398.09, 138.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1059) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (608, 1654, 4140) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11204) [M::mem_pestat] mean and std.dev: (2669.85, 2600.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14736) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (672, 1712, 4326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11634) [M::mem_pestat] mean and std.dev: (2785.60, 2663.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15288) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 265.563 CPU sec, 17.083 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3405, 91317, 3518, 3464) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (632, 1614, 3778) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10070) [M::mem_pestat] mean and std.dev: (2595.33, 2508.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13216) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (304, 383, 495) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 877) [M::mem_pestat] mean and std.dev: (400.29, 140.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1068) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (608, 1607, 4085) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11039) [M::mem_pestat] mean and std.dev: (2664.15, 2600.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14516) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (666, 1761, 4189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11235) [M::mem_pestat] mean and std.dev: (2779.64, 2613.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14758) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 263.889 CPU sec, 16.721 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3315, 91153, 3380, 3528) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (661, 1784, 4284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11530) [M::mem_pestat] mean and std.dev: (2784.09, 2618.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15153) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (305, 384, 495) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 875) [M::mem_pestat] mean and std.dev: (400.92, 140.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1065) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (578, 1648, 4274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11666) [M::mem_pestat] mean and std.dev: (2740.92, 2697.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15362) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (686, 1736, 4165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11123) [M::mem_pestat] mean and std.dev: (2735.22, 2590.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14602) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 265.345 CPU sec, 17.129 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3503, 92135, 3256, 3383) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (661, 1631, 4191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11251) [M::mem_pestat] mean and std.dev: (2691.39, 2591.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14781) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (304, 382, 494) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 874) [M::mem_pestat] mean and std.dev: (400.41, 140.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1064) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (667, 1795, 4402) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11872) [M::mem_pestat] mean and std.dev: (2828.26, 2668.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15607) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (684, 1700, 4111) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10965) [M::mem_pestat] mean and std.dev: (2739.34, 2592.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14392) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 265.326 CPU sec, 17.116 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3405, 90792, 3325, 3413) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (674, 1783, 4210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11282) [M::mem_pestat] mean and std.dev: (2782.85, 2628.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14818) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (304, 383, 492) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 868) [M::mem_pestat] mean and std.dev: (399.72, 138.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1056) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (673, 1752, 4325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11629) [M::mem_pestat] mean and std.dev: (2780.11, 2631.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15281) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (642, 1590, 4297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11607) [M::mem_pestat] mean and std.dev: (2692.18, 2578.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15262) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 264.010 CPU sec, 16.724 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3370, 91472, 3238, 3549) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (647, 1636, 4133) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11105) [M::mem_pestat] mean and std.dev: (2698.11, 2581.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14591) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 382, 494) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 876) [M::mem_pestat] mean and std.dev: (399.70, 140.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1067) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (592, 1678, 4125) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11191) [M::mem_pestat] mean and std.dev: (2681.19, 2612.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14724) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (670, 1656, 4189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11227) [M::mem_pestat] mean and std.dev: (2751.61, 2635.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14746) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 264.122 CPU sec, 16.900 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3322, 91779, 3335, 3445) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (676, 1663, 4114) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10990) [M::mem_pestat] mean and std.dev: (2721.79, 2596.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14428) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (304, 382, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 862) [M::mem_pestat] mean and std.dev: (398.59, 138.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1048) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (610, 1732, 4423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12049) [M::mem_pestat] mean and std.dev: (2779.00, 2675.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15862) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (660, 1695, 4209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11307) [M::mem_pestat] mean and std.dev: (2754.41, 2665.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14856) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 262.811 CPU sec, 16.725 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3462, 91180, 3278, 3476) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (633, 1656, 4055) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10899) [M::mem_pestat] mean and std.dev: (2649.88, 2538.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14321) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] (25, 50, 75) percentile: (304, 382, 494) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 874) [M::mem_pestat] mean and std.dev: (400.43, 139.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1064) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (576, 1520, 3976) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10776) [M::mem_pestat] mean and std.dev: (2586.28, 2602.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14176) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (660, 1666, 4349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11727) [M::mem_pestat] mean and std.dev: (2786.31, 2674.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15416) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 261.142 CPU sec, 16.739 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3551, 91411, 3318, 3511) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (647, 1653, 4175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11231) [M::mem_pestat] mean and std.dev: (2700.96, 2584.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14759) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (304, 382, 494) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 874) [M::mem_pestat] mean and std.dev: (399.94, 140.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1064) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (677, 1816, 4421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11909) [M::mem_pestat] mean and std.dev: (2839.33, 2672.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15653) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (644, 1591, 4018) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10766) [M::mem_pestat] mean and std.dev: (2663.79, 2580.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14140) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 263.328 CPU sec, 16.795 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3436, 91886, 3338, 3391) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (635, 1735, 4226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11408) [M::mem_pestat] mean and std.dev: (2741.14, 2613.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14999) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (304, 382, 493) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 871) [M::mem_pestat] mean and std.dev: (399.88, 139.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1060) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (640, 1775, 4205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11335) [M::mem_pestat] mean and std.dev: (2757.39, 2641.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14900) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (640, 1662, 4111) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11053) [M::mem_pestat] mean and std.dev: (2700.50, 2603.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14524) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 265.608 CPU sec, 17.363 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3493, 91561, 3309, 3301) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (668, 1625, 4139) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11081) [M::mem_pestat] mean and std.dev: (2638.67, 2514.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14552) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 380, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864) [M::mem_pestat] mean and std.dev: (397.55, 138.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1051) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (640, 1684, 3958) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10594) [M::mem_pestat] mean and std.dev: (2660.49, 2582.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13912) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (685, 1688, 4014) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10672) [M::mem_pestat] mean and std.dev: (2692.67, 2554.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14001) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 264.686 CPU sec, 16.887 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3389, 91426, 3188, 3495) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (628, 1620, 4138) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11158) [M::mem_pestat] mean and std.dev: (2693.46, 2616.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14668) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (304, 381, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 865) [M::mem_pestat] mean and std.dev: (397.71, 138.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1052) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (597, 1619, 3958) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10680) [M::mem_pestat] mean and std.dev: (2634.90, 2580.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14041) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (645, 1693, 4253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11469) [M::mem_pestat] mean and std.dev: (2759.18, 2661.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15077) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 264.128 CPU sec, 16.777 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3321, 91596, 3259, 3299) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (684, 1770, 4184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11184) [M::mem_pestat] mean and std.dev: (2745.56, 2574.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14684) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (304, 383, 493) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 871) [M::mem_pestat] mean and std.dev: (399.45, 139.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1060) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (640, 1629, 3946) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10558) [M::mem_pestat] mean and std.dev: (2637.28, 2565.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13864) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (657, 1629, 3902) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10392) [M::mem_pestat] mean and std.dev: (2645.79, 2549.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13637) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 265.167 CPU sec, 16.873 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3396, 91673, 3344, 3365) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (647, 1613, 4076) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10934) [M::mem_pestat] mean and std.dev: (2684.01, 2617.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14363) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (305, 383, 494) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 872) [M::mem_pestat] mean and std.dev: (400.00, 139.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1061) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (609, 1545, 3942) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10608) [M::mem_pestat] mean and std.dev: (2620.56, 2616.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13941) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (601, 1534, 3868) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10402) [M::mem_pestat] mean and std.dev: (2587.15, 2575.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13669) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 266.136 CPU sec, 17.018 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3367, 91720, 3198, 3395) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (662, 1748, 4129) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11063) [M::mem_pestat] mean and std.dev: (2760.42, 2627.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14530) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 381, 493) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 873) [M::mem_pestat] mean and std.dev: (398.88, 139.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1063) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (645, 1682, 4171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11223) [M::mem_pestat] mean and std.dev: (2725.45, 2623.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14749) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (639, 1594, 3950) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10572) [M::mem_pestat] mean and std.dev: (2635.99, 2586.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13883) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 265.594 CPU sec, 16.961 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3381, 91354, 3338, 3471) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (672, 1640, 4069) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10863) [M::mem_pestat] mean and std.dev: (2661.77, 2531.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14260) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 381, 492) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 870) [M::mem_pestat] mean and std.dev: (398.84, 139.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1059) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (644, 1826, 4302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11618) [M::mem_pestat] mean and std.dev: (2796.60, 2645.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15276) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (676, 1696, 4071) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10861) [M::mem_pestat] mean and std.dev: (2699.63, 2536.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14256) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 266.978 CPU sec, 17.148 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3397, 91971, 3304, 3413) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (634, 1680, 4273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11551) [M::mem_pestat] mean and std.dev: (2758.83, 2663.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15190) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 380, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864) [M::mem_pestat] mean and std.dev: (397.15, 138.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1051) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (623, 1657, 4158) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11228) [M::mem_pestat] mean and std.dev: (2676.35, 2576.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14763) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (624, 1677, 4108) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11076) [M::mem_pestat] mean and std.dev: (2711.93, 2602.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14560) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 266.523 CPU sec, 17.023 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3465, 91831, 3259, 3471) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (667, 1743, 4126) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11044) [M::mem_pestat] mean and std.dev: (2739.00, 2580.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14503) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 380, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864) [M::mem_pestat] mean and std.dev: (397.56, 138.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1051) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (584, 1628, 4267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11633) [M::mem_pestat] mean and std.dev: (2689.24, 2631.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15316) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (679, 1723, 4194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11224) [M::mem_pestat] mean and std.dev: (2762.43, 2620.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14739) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 266.522 CPU sec, 16.996 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3364, 91298, 3232, 3357) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (659, 1705, 4145) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11117) [M::mem_pestat] mean and std.dev: (2747.48, 2621.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14603) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 382, 492) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 870) [M::mem_pestat] mean and std.dev: (398.79, 139.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1059) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (621, 1640, 4226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11436) [M::mem_pestat] mean and std.dev: (2700.30, 2629.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15041) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (661, 1692, 4314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11620) [M::mem_pestat] mean and std.dev: (2745.29, 2623.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15273) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 266.403 CPU sec, 17.227 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3437, 91642, 3329, 3390) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (653, 1638, 4182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11240) [M::mem_pestat] mean and std.dev: (2699.95, 2617.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14769) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 381, 492) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 870) [M::mem_pestat] mean and std.dev: (398.17, 139.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1059) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (649, 1730, 4210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11332) [M::mem_pestat] mean and std.dev: (2695.72, 2565.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14893) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (639, 1568, 3968) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10626) [M::mem_pestat] mean and std.dev: (2623.69, 2555.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13955) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 263.909 CPU sec, 16.857 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3358, 91284, 3267, 3388) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (681, 1761, 4224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11310) [M::mem_pestat] mean and std.dev: (2773.78, 2618.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14853) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 380, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 869) [M::mem_pestat] mean and std.dev: (397.43, 139.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1058) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (636, 1716, 4267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11529) [M::mem_pestat] mean and std.dev: (2726.52, 2626.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15160) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (639, 1665, 4119) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11079) [M::mem_pestat] mean and std.dev: (2755.37, 2675.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14559) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 265.673 CPU sec, 16.923 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3418, 91257, 3333, 3452) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (691, 1731, 4186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11176) [M::mem_pestat] mean and std.dev: (2744.40, 2604.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14671) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 380, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864) [M::mem_pestat] mean and std.dev: (396.98, 138.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1051) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (606, 1640, 4125) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11163) [M::mem_pestat] mean and std.dev: (2669.51, 2595.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14682) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (609, 1644, 4252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11538) [M::mem_pestat] mean and std.dev: (2733.46, 2646.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15181) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 261.783 CPU sec, 16.753 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3405, 91091, 3314, 3456) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (662, 1606, 4149) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11123) [M::mem_pestat] mean and std.dev: (2716.35, 2621.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14610) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 380, 492) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 870) [M::mem_pestat] mean and std.dev: (398.25, 139.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1059) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (617, 1728, 4287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11627) [M::mem_pestat] mean and std.dev: (2731.74, 2609.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15297) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (641, 1602, 3933) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10517) [M::mem_pestat] mean and std.dev: (2624.69, 2541.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13809) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 267.051 CPU sec, 16.986 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3464, 91335, 3165, 3443) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (656, 1709, 4244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11420) [M::mem_pestat] mean and std.dev: (2787.34, 2660.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15008) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 380, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 869) [M::mem_pestat] mean and std.dev: (397.95, 139.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1058) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (651, 1721, 4204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11310) [M::mem_pestat] mean and std.dev: (2754.66, 2629.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14863) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (703, 1714, 4226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11272) [M::mem_pestat] mean and std.dev: (2764.41, 2606.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14795) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 268.623 CPU sec, 17.095 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3324, 91501, 3267, 3475) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (647, 1665, 4078) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10940) [M::mem_pestat] mean and std.dev: (2684.07, 2599.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14371) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 381, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 863) [M::mem_pestat] mean and std.dev: (397.03, 138.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1050) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (658, 1706, 4200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11284) [M::mem_pestat] mean and std.dev: (2744.96, 2633.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14826) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (666, 1613, 4036) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10776) [M::mem_pestat] mean and std.dev: (2666.73, 2552.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14146) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 267.603 CPU sec, 17.030 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3205, 91767, 3251, 3382) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (651, 1745, 4242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11424) [M::mem_pestat] mean and std.dev: (2764.23, 2630.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15015) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (301, 380, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 865) [M::mem_pestat] mean and std.dev: (396.60, 138.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1053) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (616, 1572, 3984) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10720) [M::mem_pestat] mean and std.dev: (2614.55, 2550.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14088) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (669, 1756, 4062) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10848) [M::mem_pestat] mean and std.dev: (2713.80, 2573.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14241) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 267.914 CPU sec, 17.035 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3427, 91364, 3417, 3392) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (626, 1593, 3994) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10730) [M::mem_pestat] mean and std.dev: (2637.86, 2573.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14098) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 381, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 867) [M::mem_pestat] mean and std.dev: (398.58, 139.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1055) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (588, 1610, 3978) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10758) [M::mem_pestat] mean and std.dev: (2606.72, 2585.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14148) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (617, 1580, 4167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11267) [M::mem_pestat] mean and std.dev: (2650.90, 2581.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14817) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 267.336 CPU sec, 17.001 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3460, 91154, 3320, 3456) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (634, 1673, 4189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11299) [M::mem_pestat] mean and std.dev: (2688.11, 2578.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14854) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 380, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 866) [M::mem_pestat] mean and std.dev: (397.10, 139.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1054) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (603, 1638, 4033) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10893) [M::mem_pestat] mean and std.dev: (2661.25, 2621.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14323) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (624, 1597, 4003) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10761) [M::mem_pestat] mean and std.dev: (2643.81, 2580.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14140) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 264.633 CPU sec, 16.841 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3403, 91687, 3373, 3381) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (661, 1700, 4151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11131) [M::mem_pestat] mean and std.dev: (2708.44, 2584.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14621) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (301, 379, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 865) [M::mem_pestat] mean and std.dev: (396.80, 138.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1053) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (641, 1674, 4153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11177) [M::mem_pestat] mean and std.dev: (2686.58, 2596.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14689) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (641, 1677, 4250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11468) [M::mem_pestat] mean and std.dev: (2728.89, 2623.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15077) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 265.504 CPU sec, 16.946 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3384, 91098, 3389, 3497) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (662, 1630, 4269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11483) [M::mem_pestat] mean and std.dev: (2697.29, 2588.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15090) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (301, 379, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 865) [M::mem_pestat] mean and std.dev: (396.44, 139.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1053) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (602, 1637, 4069) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11003) [M::mem_pestat] mean and std.dev: (2655.56, 2578.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14470) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (654, 1581, 3996) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10680) [M::mem_pestat] mean and std.dev: (2644.52, 2565.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14022) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 266.853 CPU sec, 17.313 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3244, 91219, 3306, 3522) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (639, 1649, 4020) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10782) [M::mem_pestat] mean and std.dev: (2713.39, 2634.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14163) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 381, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 867) [M::mem_pestat] mean and std.dev: (398.27, 138.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1055) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (610, 1666, 4198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11374) [M::mem_pestat] mean and std.dev: (2717.00, 2626.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14962) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (623, 1601, 4090) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11024) [M::mem_pestat] mean and std.dev: (2654.54, 2581.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14491) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 266.634 CPU sec, 16.987 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3287, 91730, 3319, 3351) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (674, 1667, 3938) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10466) [M::mem_pestat] mean and std.dev: (2610.09, 2474.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13730) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 380, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 866) [M::mem_pestat] mean and std.dev: (397.70, 139.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1054) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (609, 1691, 4223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11451) [M::mem_pestat] mean and std.dev: (2736.27, 2659.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15065) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (692, 1690, 4113) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10955) [M::mem_pestat] mean and std.dev: (2731.00, 2608.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14376) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 265.358 CPU sec, 16.906 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3374, 92003, 3250, 3387) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (673, 1773, 4236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11362) [M::mem_pestat] mean and std.dev: (2732.50, 2547.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14925) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 380, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 869) [M::mem_pestat] mean and std.dev: (397.86, 139.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1058) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (616, 1688, 4251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11521) [M::mem_pestat] mean and std.dev: (2705.93, 2587.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15156) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (632, 1643, 4104) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11048) [M::mem_pestat] mean and std.dev: (2693.01, 2598.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14520) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 265.338 CPU sec, 16.931 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3504, 91593, 3284, 3397) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (653, 1646, 3996) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10682) [M::mem_pestat] mean and std.dev: (2644.43, 2518.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14025) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 380, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 866) [M::mem_pestat] mean and std.dev: (397.49, 139.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1054) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (621, 1714, 4279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11595) [M::mem_pestat] mean and std.dev: (2746.63, 2662.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15253) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (649, 1643, 4153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11161) [M::mem_pestat] mean and std.dev: (2720.35, 2620.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14665) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 268.319 CPU sec, 17.054 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3405, 92391, 3434, 3405) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (630, 1766, 4335) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11745) [M::mem_pestat] mean and std.dev: (2772.50, 2655.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15450) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 380, 487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 857) [M::mem_pestat] mean and std.dev: (396.50, 137.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1042) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (622, 1650, 4111) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11089) [M::mem_pestat] mean and std.dev: (2669.70, 2597.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14578) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (644, 1640, 4108) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11036) [M::mem_pestat] mean and std.dev: (2680.33, 2591.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14500) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 262.693 CPU sec, 16.743 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3463, 92311, 3502, 3509) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (656, 1685, 4193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11267) [M::mem_pestat] mean and std.dev: (2736.78, 2624.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14804) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 378, 487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 857) [M::mem_pestat] mean and std.dev: (395.39, 136.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1042) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (659, 1647, 4150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11132) [M::mem_pestat] mean and std.dev: (2722.64, 2631.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14623) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (612, 1633, 4114) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11118) [M::mem_pestat] mean and std.dev: (2687.77, 2597.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14620) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 264.013 CPU sec, 16.963 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3420, 92466, 3317, 3363) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (670, 1679, 4260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11440) [M::mem_pestat] mean and std.dev: (2764.09, 2650.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15030) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 380, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861) [M::mem_pestat] mean and std.dev: (396.72, 137.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1047) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (591, 1714, 4160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11298) [M::mem_pestat] mean and std.dev: (2724.95, 2667.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14867) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (660, 1625, 3924) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10452) [M::mem_pestat] mean and std.dev: (2638.80, 2539.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13716) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 263.256 CPU sec, 17.067 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3425, 91985, 3180, 3378) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (639, 1608, 4249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11469) [M::mem_pestat] mean and std.dev: (2706.17, 2597.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15079) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (301, 378, 487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 859) [M::mem_pestat] mean and std.dev: (395.18, 137.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1045) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (665, 1771, 4147) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11111) [M::mem_pestat] mean and std.dev: (2717.46, 2582.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14593) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (601, 1508, 3989) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10765) [M::mem_pestat] mean and std.dev: (2617.31, 2575.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14153) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 264.596 CPU sec, 16.831 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3492, 91889, 3210, 3359) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (633, 1653, 4209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11361) [M::mem_pestat] mean and std.dev: (2762.81, 2677.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14937) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 379, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860) [M::mem_pestat] mean and std.dev: (396.68, 137.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1046) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (650, 1729, 3981) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10643) [M::mem_pestat] mean and std.dev: (2688.35, 2578.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13974) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (593, 1572, 4105) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11129) [M::mem_pestat] mean and std.dev: (2676.11, 2629.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14641) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 265.233 CPU sec, 16.885 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3449, 92401, 3443, 3526) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (597, 1560, 3814) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10248) [M::mem_pestat] mean and std.dev: (2615.19, 2584.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13465) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (301, 379, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 868) [M::mem_pestat] mean and std.dev: (397.12, 138.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1057) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (615, 1756, 4276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11598) [M::mem_pestat] mean and std.dev: (2780.02, 2666.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15259) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (688, 1651, 3831) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10117) [M::mem_pestat] mean and std.dev: (2574.77, 2429.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13260) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 262.626 CPU sec, 16.757 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3354, 92158, 3232, 3367) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (635, 1542, 4074) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10952) [M::mem_pestat] mean and std.dev: (2631.58, 2561.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14391) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 380, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 866) [M::mem_pestat] mean and std.dev: (397.33, 138.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1054) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (657, 1756, 4135) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11091) [M::mem_pestat] mean and std.dev: (2717.24, 2571.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14569) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (641, 1606, 3883) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10367) [M::mem_pestat] mean and std.dev: (2649.63, 2575.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13609) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 263.576 CPU sec, 16.717 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3521, 92309, 3340, 3475) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (631, 1696, 4219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11395) [M::mem_pestat] mean and std.dev: (2706.89, 2597.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14983) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (304, 380, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 865) [M::mem_pestat] mean and std.dev: (398.02, 138.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1052) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (582, 1681, 4173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11355) [M::mem_pestat] mean and std.dev: (2698.46, 2611.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14946) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (637, 1651, 4026) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10804) [M::mem_pestat] mean and std.dev: (2671.22, 2604.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14193) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 263.826 CPU sec, 16.935 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3420, 91733, 3443, 3570) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (624, 1630, 4145) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11187) [M::mem_pestat] mean and std.dev: (2695.58, 2613.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14708) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 381, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 867) [M::mem_pestat] mean and std.dev: (398.18, 139.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1055) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (662, 1839, 4460) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12056) [M::mem_pestat] mean and std.dev: (2829.52, 2638.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15854) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (655, 1745, 4171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11203) [M::mem_pestat] mean and std.dev: (2729.19, 2593.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14719) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 263.433 CPU sec, 16.842 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3544, 91944, 3280, 3455) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (658, 1713, 4095) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10969) [M::mem_pestat] mean and std.dev: (2727.39, 2580.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14406) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 381, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 867) [M::mem_pestat] mean and std.dev: (398.32, 138.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1055) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (624, 1718, 4306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11670) [M::mem_pestat] mean and std.dev: (2727.65, 2625.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15352) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (624, 1594, 3823) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10221) [M::mem_pestat] mean and std.dev: (2634.80, 2577.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13420) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 263.916 CPU sec, 16.985 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3406, 91631, 3221, 3566) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (672, 1644, 3999) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10653) [M::mem_pestat] mean and std.dev: (2642.89, 2527.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13980) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 381, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864) [M::mem_pestat] mean and std.dev: (398.02, 138.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1051) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (594, 1643, 4112) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11148) [M::mem_pestat] mean and std.dev: (2675.79, 2627.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14666) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (620, 1611, 4103) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11069) [M::mem_pestat] mean and std.dev: (2690.37, 2644.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14552) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 263.207 CPU sec, 16.705 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3428, 91474, 3304, 3452) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (657, 1733, 4440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12006) [M::mem_pestat] mean and std.dev: (2784.86, 2632.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15789) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 381, 492) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 870) [M::mem_pestat] mean and std.dev: (399.09, 139.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1059) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (654, 1751, 4256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11460) [M::mem_pestat] mean and std.dev: (2768.79, 2653.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15062) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (633, 1675, 4049) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10881) [M::mem_pestat] mean and std.dev: (2683.41, 2574.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14297) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 264.753 CPU sec, 16.820 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3458, 91688, 3221, 3653) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (641, 1641, 4057) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10889) [M::mem_pestat] mean and std.dev: (2665.94, 2571.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14305) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 380, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864) [M::mem_pestat] mean and std.dev: (397.68, 138.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1051) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (622, 1638, 4264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11548) [M::mem_pestat] mean and std.dev: (2723.79, 2645.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15190) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (662, 1682, 4142) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11102) [M::mem_pestat] mean and std.dev: (2710.38, 2585.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14582) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 263.919 CPU sec, 16.921 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3573, 91647, 3421, 3363) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (670, 1740, 4344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11692) [M::mem_pestat] mean and std.dev: (2810.73, 2672.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15366) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 381, 492) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 870) [M::mem_pestat] mean and std.dev: (398.52, 139.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1059) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (584, 1564, 3884) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10484) [M::mem_pestat] mean and std.dev: (2589.05, 2556.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13784) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (645, 1569, 3940) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10530) [M::mem_pestat] mean and std.dev: (2634.81, 2582.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13825) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 264.981 CPU sec, 16.990 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3498, 92085, 3447, 3398) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (653, 1776, 4157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11165) [M::mem_pestat] mean and std.dev: (2751.40, 2593.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14669) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (305, 383, 494) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 872) [M::mem_pestat] mean and std.dev: (401.07, 139.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1061) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (621, 1578, 3991) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10731) [M::mem_pestat] mean and std.dev: (2623.06, 2576.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14101) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (610, 1642, 4007) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10801) [M::mem_pestat] mean and std.dev: (2621.38, 2537.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14198) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 264.716 CPU sec, 16.924 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3483, 92149, 3345, 3549) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (704, 1812, 4331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11585) [M::mem_pestat] mean and std.dev: (2819.04, 2647.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15212) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (304, 383, 493) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 871) [M::mem_pestat] mean and std.dev: (399.39, 138.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1060) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (603, 1643, 4152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11250) [M::mem_pestat] mean and std.dev: (2669.57, 2574.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14799) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (591, 1579, 3973) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10737) [M::mem_pestat] mean and std.dev: (2636.35, 2594.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14119) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 263.962 CPU sec, 16.855 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3450, 91893, 3250, 3419) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (629, 1591, 4022) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10808) [M::mem_pestat] mean and std.dev: (2638.66, 2548.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14201) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (304, 381, 492) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 868) [M::mem_pestat] mean and std.dev: (398.84, 139.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1056) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (628, 1654, 4489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12211) [M::mem_pestat] mean and std.dev: (2779.08, 2644.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16072) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (645, 1620, 3991) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10683) [M::mem_pestat] mean and std.dev: (2682.15, 2599.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14029) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 264.873 CPU sec, 16.873 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3575, 91664, 3346, 3365) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (668, 1603, 4101) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10967) [M::mem_pestat] mean and std.dev: (2662.11, 2560.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14400) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (304, 382, 492) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 868) [M::mem_pestat] mean and std.dev: (399.14, 138.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1056) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (640, 1672, 4509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12247) [M::mem_pestat] mean and std.dev: (2807.23, 2716.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16116) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (634, 1693, 4077) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10963) [M::mem_pestat] mean and std.dev: (2726.78, 2664.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14406) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 264.413 CPU sec, 16.890 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3453, 91379, 3353, 3562) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (704, 1670, 4107) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10913) [M::mem_pestat] mean and std.dev: (2711.48, 2577.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14316) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (304, 382, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 862) [M::mem_pestat] mean and std.dev: (398.05, 137.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1048) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (657, 1720, 4335) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11691) [M::mem_pestat] mean and std.dev: (2794.55, 2682.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15369) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (615, 1611, 3996) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10758) [M::mem_pestat] mean and std.dev: (2640.63, 2576.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14139) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 266.068 CPU sec, 16.935 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3394, 91859, 3214, 3382) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (678, 1693, 4154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11106) [M::mem_pestat] mean and std.dev: (2733.09, 2608.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14582) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (305, 382, 492) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 866) [M::mem_pestat] mean and std.dev: (399.03, 138.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1053) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (614, 1650, 4065) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10967) [M::mem_pestat] mean and std.dev: (2648.36, 2586.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14418) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (608, 1552, 4095) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11069) [M::mem_pestat] mean and std.dev: (2678.07, 2634.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14556) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 265.346 CPU sec, 16.898 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3291, 91773, 3336, 3320) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (649, 1660, 4108) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11026) [M::mem_pestat] mean and std.dev: (2706.93, 2607.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14485) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 382, 492) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 870) [M::mem_pestat] mean and std.dev: (398.62, 138.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1059) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (594, 1618, 4045) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10947) [M::mem_pestat] mean and std.dev: (2695.53, 2657.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14398) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (651, 1665, 3987) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10659) [M::mem_pestat] mean and std.dev: (2668.21, 2587.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13995) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 263.762 CPU sec, 16.779 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3366, 91490, 3258, 3406) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (645, 1664, 3937) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10521) [M::mem_pestat] mean and std.dev: (2698.27, 2609.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13813) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (305, 382, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 863) [M::mem_pestat] mean and std.dev: (398.92, 137.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1049) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (625, 1724, 4211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11383) [M::mem_pestat] mean and std.dev: (2732.13, 2644.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14969) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (595, 1528, 3968) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10714) [M::mem_pestat] mean and std.dev: (2616.33, 2601.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14087) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 269.042 CPU sec, 17.089 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3547, 91815, 3217, 3466) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (634, 1610, 4240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11452) [M::mem_pestat] mean and std.dev: (2729.75, 2640.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15058) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (304, 382, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 865) [M::mem_pestat] mean and std.dev: (398.51, 138.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1052) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (609, 1701, 4299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11679) [M::mem_pestat] mean and std.dev: (2718.59, 2629.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15369) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (658, 1666, 4186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11242) [M::mem_pestat] mean and std.dev: (2750.30, 2633.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14770) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 268.342 CPU sec, 17.136 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3441, 91652, 3261, 3414) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (657, 1721, 4066) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10884) [M::mem_pestat] mean and std.dev: (2689.48, 2574.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14293) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (304, 382, 494) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 874) [M::mem_pestat] mean and std.dev: (399.67, 139.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1064) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (614, 1708, 4165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11267) [M::mem_pestat] mean and std.dev: (2713.70, 2618.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14818) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (651, 1583, 4182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11244) [M::mem_pestat] mean and std.dev: (2686.34, 2606.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14775) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 266.554 CPU sec, 17.324 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3296, 91265, 3333, 3493) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (649, 1662, 4036) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10810) [M::mem_pestat] mean and std.dev: (2665.78, 2538.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14197) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (304, 382, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 865) [M::mem_pestat] mean and std.dev: (398.97, 138.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1052) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (666, 1776, 4190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11238) [M::mem_pestat] mean and std.dev: (2766.87, 2640.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14762) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (637, 1574, 3947) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10567) [M::mem_pestat] mean and std.dev: (2643.42, 2572.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13877) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 267.609 CPU sec, 17.080 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3513, 92092, 3282, 3440) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (671, 1719, 4345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11693) [M::mem_pestat] mean and std.dev: (2826.93, 2699.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15367) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (304, 381, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 859) [M::mem_pestat] mean and std.dev: (397.57, 136.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1044) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (631, 1727, 4261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11521) [M::mem_pestat] mean and std.dev: (2747.41, 2647.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15151) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (673, 1720, 4090) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10924) [M::mem_pestat] mean and std.dev: (2757.28, 2662.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14341) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 266.113 CPU sec, 16.936 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3477, 93046, 3337, 3448) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (659, 1661, 4135) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11087) [M::mem_pestat] mean and std.dev: (2692.27, 2577.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14563) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 378, 483) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 845) [M::mem_pestat] mean and std.dev: (394.08, 133.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1026) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (589, 1619, 4225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11497) [M::mem_pestat] mean and std.dev: (2710.36, 2678.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15133) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (645, 1679, 4175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11235) [M::mem_pestat] mean and std.dev: (2704.36, 2600.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14765) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 270.884 CPU sec, 17.237 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3402, 92158, 3288, 3427) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (646, 1618, 4142) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11134) [M::mem_pestat] mean and std.dev: (2694.73, 2624.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14630) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (304, 380, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 859) [M::mem_pestat] mean and std.dev: (397.33, 136.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1044) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (615, 1654, 4176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11298) [M::mem_pestat] mean and std.dev: (2697.06, 2625.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14859) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (666, 1643, 4203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11277) [M::mem_pestat] mean and std.dev: (2754.58, 2651.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14814) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 269.666 CPU sec, 17.282 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3470, 91319, 3185, 3487) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (665, 1668, 4213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11309) [M::mem_pestat] mean and std.dev: (2698.08, 2562.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14857) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (304, 381, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 865) [M::mem_pestat] mean and std.dev: (398.54, 138.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1052) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (648, 1728, 4300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11604) [M::mem_pestat] mean and std.dev: (2759.62, 2624.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15256) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (629, 1645, 4103) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11051) [M::mem_pestat] mean and std.dev: (2654.25, 2551.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14525) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 267.635 CPU sec, 17.034 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3521, 92083, 3257, 3290) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (653, 1637, 4288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11558) [M::mem_pestat] mean and std.dev: (2749.19, 2667.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15193) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (304, 383, 494) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 874) [M::mem_pestat] mean and std.dev: (400.67, 140.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1064) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (653, 1655, 4314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11636) [M::mem_pestat] mean and std.dev: (2750.69, 2659.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15297) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (621, 1589, 4013) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10797) [M::mem_pestat] mean and std.dev: (2654.12, 2582.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14189) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 265.931 CPU sec, 16.917 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3524, 91319, 3362, 3453) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (686, 1736, 4204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11240) [M::mem_pestat] mean and std.dev: (2764.34, 2610.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14758) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (305, 382, 492) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 866) [M::mem_pestat] mean and std.dev: (399.43, 139.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1053) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (634, 1732, 4230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11422) [M::mem_pestat] mean and std.dev: (2735.57, 2644.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15018) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (627, 1750, 4232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11442) [M::mem_pestat] mean and std.dev: (2745.00, 2612.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15047) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 267.760 CPU sec, 17.012 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3465, 90701, 3335, 3488) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (638, 1599, 4057) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10895) [M::mem_pestat] mean and std.dev: (2667.17, 2576.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14314) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 382, 493) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 873) [M::mem_pestat] mean and std.dev: (399.65, 140.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1063) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (623, 1813, 4151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11207) [M::mem_pestat] mean and std.dev: (2758.98, 2639.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14735) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (617, 1623, 4193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11345) [M::mem_pestat] mean and std.dev: (2734.33, 2637.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14921) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 266.045 CPU sec, 16.942 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3387, 91107, 3302, 3434) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (650, 1695, 3917) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10451) [M::mem_pestat] mean and std.dev: (2652.86, 2561.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13718) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (304, 382, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 865) [M::mem_pestat] mean and std.dev: (398.96, 138.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1052) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (578, 1566, 3951) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10697) [M::mem_pestat] mean and std.dev: (2612.27, 2582.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14070) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (666, 1662, 4128) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11052) [M::mem_pestat] mean and std.dev: (2699.21, 2610.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14514) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 267.964 CPU sec, 17.114 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3441, 91916, 3288, 3462) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (689, 1788, 4261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11405) [M::mem_pestat] mean and std.dev: (2782.33, 2595.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14977) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (304, 382, 494) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 874) [M::mem_pestat] mean and std.dev: (400.07, 140.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1064) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (607, 1621, 4071) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10999) [M::mem_pestat] mean and std.dev: (2644.44, 2601.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14463) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (651, 1620, 3961) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10581) [M::mem_pestat] mean and std.dev: (2668.47, 2620.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13891) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 268.915 CPU sec, 17.099 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3412, 91928, 3059, 3303) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (671, 1698, 4321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11621) [M::mem_pestat] mean and std.dev: (2783.90, 2703.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15271) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (304, 381, 492) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 868) [M::mem_pestat] mean and std.dev: (398.55, 138.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1056) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (597, 1636, 4147) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11247) [M::mem_pestat] mean and std.dev: (2679.33, 2626.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14797) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (652, 1683, 4184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11248) [M::mem_pestat] mean and std.dev: (2732.33, 2615.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14780) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 266.499 CPU sec, 16.907 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3420, 92047, 3359, 3425) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (609, 1599, 4050) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10932) [M::mem_pestat] mean and std.dev: (2665.40, 2600.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14373) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 381, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 867) [M::mem_pestat] mean and std.dev: (398.43, 138.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1055) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (644, 1776, 4340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11732) [M::mem_pestat] mean and std.dev: (2776.48, 2654.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15428) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (648, 1570, 4058) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10878) [M::mem_pestat] mean and std.dev: (2641.83, 2583.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14288) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 264.288 CPU sec, 16.813 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3460, 91726, 3249, 3393) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (617, 1536, 4025) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10841) [M::mem_pestat] mean and std.dev: (2644.86, 2615.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14249) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (304, 381, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 865) [M::mem_pestat] mean and std.dev: (398.40, 138.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1052) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (602, 1583, 4234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11498) [M::mem_pestat] mean and std.dev: (2693.70, 2650.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15130) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (651, 1764, 4310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11628) [M::mem_pestat] mean and std.dev: (2750.35, 2595.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15287) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 266.739 CPU sec, 16.959 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3489, 91535, 3430, 3471) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (657, 1710, 4221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11349) [M::mem_pestat] mean and std.dev: (2721.37, 2591.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14913) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (304, 383, 493) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 871) [M::mem_pestat] mean and std.dev: (399.75, 139.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1060) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (625, 1673, 4060) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10930) [M::mem_pestat] mean and std.dev: (2665.52, 2604.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14365) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (675, 1693, 4208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11274) [M::mem_pestat] mean and std.dev: (2756.42, 2632.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14807) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 267.279 CPU sec, 17.011 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3377, 92010, 3301, 3267) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (633, 1588, 4070) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10944) [M::mem_pestat] mean and std.dev: (2637.06, 2568.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14381) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 381, 492) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 870) [M::mem_pestat] mean and std.dev: (398.59, 139.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1059) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (629, 1802, 4480) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12182) [M::mem_pestat] mean and std.dev: (2826.69, 2680.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16033) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (633, 1703, 4040) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10854) [M::mem_pestat] mean and std.dev: (2689.71, 2589.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14261) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 265.844 CPU sec, 17.103 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3445, 91986, 3355, 3485) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (664, 1666, 4493) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12151) [M::mem_pestat] mean and std.dev: (2840.55, 2692.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15980) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (304, 382, 492) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 868) [M::mem_pestat] mean and std.dev: (399.45, 138.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1056) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (628, 1699, 4303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11653) [M::mem_pestat] mean and std.dev: (2783.47, 2679.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15328) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (665, 1610, 3940) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10490) [M::mem_pestat] mean and std.dev: (2652.18, 2559.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13765) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 267.196 CPU sec, 16.993 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3682, 91480, 3336, 3374) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (628, 1553, 3933) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10543) [M::mem_pestat] mean and std.dev: (2620.22, 2587.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13848) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 381, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 867) [M::mem_pestat] mean and std.dev: (398.43, 138.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1055) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (662, 1745, 4432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11972) [M::mem_pestat] mean and std.dev: (2805.77, 2674.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15742) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (696, 1636, 4010) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10638) [M::mem_pestat] mean and std.dev: (2681.92, 2567.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13952) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 268.443 CPU sec, 17.076 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3406, 91657, 3227, 3164) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (673, 1721, 4263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11443) [M::mem_pestat] mean and std.dev: (2767.22, 2627.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15033) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (304, 381, 492) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 868) [M::mem_pestat] mean and std.dev: (399.05, 139.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1056) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (620, 1672, 4365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11855) [M::mem_pestat] mean and std.dev: (2778.61, 2704.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15600) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (673, 1667, 4306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11572) [M::mem_pestat] mean and std.dev: (2765.13, 2625.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15205) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 269.376 CPU sec, 17.135 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3479, 91571, 3224, 3391) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (669, 1644, 4133) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11061) [M::mem_pestat] mean and std.dev: (2706.98, 2628.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14525) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (304, 382, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 865) [M::mem_pestat] mean and std.dev: (398.55, 138.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1052) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (606, 1658, 4153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11247) [M::mem_pestat] mean and std.dev: (2701.61, 2621.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14794) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (614, 1633, 4071) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10985) [M::mem_pestat] mean and std.dev: (2664.27, 2594.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14442) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 269.178 CPU sec, 17.132 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3466, 91069, 3319, 3469) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (653, 1663, 4107) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11015) [M::mem_pestat] mean and std.dev: (2705.29, 2610.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14469) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (304, 381, 492) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 868) [M::mem_pestat] mean and std.dev: (398.39, 138.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1056) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (632, 1687, 4322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11702) [M::mem_pestat] mean and std.dev: (2776.19, 2710.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15392) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (622, 1561, 4050) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10906) [M::mem_pestat] mean and std.dev: (2657.40, 2605.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14334) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 264.306 CPU sec, 16.828 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3492, 91705, 3231, 3470) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (625, 1648, 4173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11269) [M::mem_pestat] mean and std.dev: (2710.45, 2634.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14817) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 381, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864) [M::mem_pestat] mean and std.dev: (397.91, 137.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1051) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (645, 1722, 4263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11499) [M::mem_pestat] mean and std.dev: (2757.26, 2646.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15117) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (667, 1635, 4208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11290) [M::mem_pestat] mean and std.dev: (2704.44, 2611.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14831) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 265.624 CPU sec, 16.880 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3391, 91971, 3312, 3361) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (669, 1605, 4045) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10797) [M::mem_pestat] mean and std.dev: (2668.44, 2574.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14173) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 381, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864) [M::mem_pestat] mean and std.dev: (397.66, 138.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1051) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (582, 1603, 4077) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11067) [M::mem_pestat] mean and std.dev: (2623.68, 2582.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14562) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (646, 1600, 4058) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10882) [M::mem_pestat] mean and std.dev: (2649.71, 2548.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14294) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 266.456 CPU sec, 16.939 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3527, 91768, 3381, 3582) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (662, 1692, 4104) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10988) [M::mem_pestat] mean and std.dev: (2685.77, 2558.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14430) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 381, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 869) [M::mem_pestat] mean and std.dev: (397.89, 139.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1058) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (590, 1643, 4247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11561) [M::mem_pestat] mean and std.dev: (2712.19, 2649.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15218) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (611, 1651, 4126) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11156) [M::mem_pestat] mean and std.dev: (2676.05, 2576.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14671) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 268.475 CPU sec, 17.230 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3350, 91600, 3200, 3408) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (662, 1609, 4042) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10802) [M::mem_pestat] mean and std.dev: (2677.01, 2580.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14182) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 380, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 863) [M::mem_pestat] mean and std.dev: (397.06, 138.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1050) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (600, 1708, 4098) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11094) [M::mem_pestat] mean and std.dev: (2705.07, 2631.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14592) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (631, 1718, 4237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11449) [M::mem_pestat] mean and std.dev: (2752.90, 2634.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15055) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 267.504 CPU sec, 17.043 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3351, 92309, 3306, 3545) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (623, 1561, 3956) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10622) [M::mem_pestat] mean and std.dev: (2654.22, 2587.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13955) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 381, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 867) [M::mem_pestat] mean and std.dev: (398.11, 138.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1055) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (608, 1659, 4376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11912) [M::mem_pestat] mean and std.dev: (2754.99, 2690.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15680) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (642, 1706, 4166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11214) [M::mem_pestat] mean and std.dev: (2713.10, 2572.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14738) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 266.905 CPU sec, 17.421 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3210, 91448, 3211, 3370) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (646, 1653, 4126) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11086) [M::mem_pestat] mean and std.dev: (2741.88, 2652.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14566) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 381, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 867) [M::mem_pestat] mean and std.dev: (398.41, 138.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1055) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (644, 1755, 4460) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12092) [M::mem_pestat] mean and std.dev: (2791.97, 2634.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15908) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (677, 1751, 4035) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10751) [M::mem_pestat] mean and std.dev: (2695.33, 2559.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14109) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 267.223 CPU sec, 17.092 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3442, 91871, 3301, 3396) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (622, 1654, 4192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11332) [M::mem_pestat] mean and std.dev: (2724.29, 2637.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14902) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 381, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864) [M::mem_pestat] mean and std.dev: (397.51, 137.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1051) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (613, 1599, 4174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11296) [M::mem_pestat] mean and std.dev: (2695.79, 2625.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14857) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (656, 1726, 4013) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10727) [M::mem_pestat] mean and std.dev: (2731.36, 2611.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14084) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 267.178 CPU sec, 16.997 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3477, 92153, 3289, 3512) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (636, 1618, 3958) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10602) [M::mem_pestat] mean and std.dev: (2659.19, 2584.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13924) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 380, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 867) [M::mem_pestat] mean and std.dev: (398.19, 138.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1055) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (595, 1635, 4081) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11053) [M::mem_pestat] mean and std.dev: (2690.19, 2670.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14539) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (656, 1682, 4077) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10919) [M::mem_pestat] mean and std.dev: (2698.37, 2560.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14340) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 266.023 CPU sec, 16.932 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3517, 92192, 3239, 3335) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (612, 1543, 3949) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10623) [M::mem_pestat] mean and std.dev: (2602.65, 2590.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13960) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 380, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861) [M::mem_pestat] mean and std.dev: (397.15, 138.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1047) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (584, 1593, 3880) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10472) [M::mem_pestat] mean and std.dev: (2567.33, 2515.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13768) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (689, 1647, 4108) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10946) [M::mem_pestat] mean and std.dev: (2732.07, 2615.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14365) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 266.480 CPU sec, 16.941 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3444, 91826, 3383, 3530) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (677, 1718, 4302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11552) [M::mem_pestat] mean and std.dev: (2787.69, 2670.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15177) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 380, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 867) [M::mem_pestat] mean and std.dev: (397.81, 138.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1055) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (625, 1695, 4230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11440) [M::mem_pestat] mean and std.dev: (2715.54, 2625.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15045) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (618, 1565, 3923) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10533) [M::mem_pestat] mean and std.dev: (2610.21, 2535.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13838) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 266.213 CPU sec, 16.923 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3388, 91641, 3253, 3451) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (627, 1537, 4126) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11124) [M::mem_pestat] mean and std.dev: (2679.76, 2618.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14623) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 380, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 866) [M::mem_pestat] mean and std.dev: (397.19, 138.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1054) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (600, 1725, 4132) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11196) [M::mem_pestat] mean and std.dev: (2696.60, 2605.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14728) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (632, 1657, 4120) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11096) [M::mem_pestat] mean and std.dev: (2706.82, 2615.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14584) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 267.217 CPU sec, 17.208 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3346, 91922, 3225, 3371) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (641, 1642, 4075) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10943) [M::mem_pestat] mean and std.dev: (2688.49, 2609.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14377) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 379, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861) [M::mem_pestat] mean and std.dev: (396.97, 138.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1047) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (625, 1632, 3938) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10564) [M::mem_pestat] mean and std.dev: (2616.10, 2522.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13877) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (640, 1624, 4040) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10840) [M::mem_pestat] mean and std.dev: (2713.05, 2625.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14240) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 267.266 CPU sec, 17.092 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3373, 91573, 3403, 3345) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (636, 1634, 4002) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10734) [M::mem_pestat] mean and std.dev: (2640.17, 2555.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14100) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 381, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 867) [M::mem_pestat] mean and std.dev: (398.07, 138.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1055) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (648, 1687, 4423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11973) [M::mem_pestat] mean and std.dev: (2774.98, 2672.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15748) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (649, 1620, 4003) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10711) [M::mem_pestat] mean and std.dev: (2700.52, 2624.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14065) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 266.685 CPU sec, 16.969 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3550, 91288, 3369, 3574) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (668, 1538, 3962) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10550) [M::mem_pestat] mean and std.dev: (2630.08, 2566.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13844) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 380, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 866) [M::mem_pestat] mean and std.dev: (397.26, 138.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1054) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (661, 1761, 4253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11437) [M::mem_pestat] mean and std.dev: (2757.04, 2632.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15029) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (651, 1562, 4079) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10935) [M::mem_pestat] mean and std.dev: (2662.74, 2590.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14363) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 267.450 CPU sec, 17.040 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3533, 91527, 3234, 3376) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (643, 1622, 4061) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10897) [M::mem_pestat] mean and std.dev: (2691.37, 2609.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14315) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 380, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 866) [M::mem_pestat] mean and std.dev: (397.53, 138.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1054) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (618, 1694, 4208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11388) [M::mem_pestat] mean and std.dev: (2683.51, 2605.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14978) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (629, 1576, 3915) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10487) [M::mem_pestat] mean and std.dev: (2659.84, 2597.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13773) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 268.093 CPU sec, 17.151 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3444, 91162, 3179, 3362) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (619, 1598, 4215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11407) [M::mem_pestat] mean and std.dev: (2692.20, 2619.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15003) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 380, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 866) [M::mem_pestat] mean and std.dev: (397.38, 139.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1054) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (629, 1711, 4327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11723) [M::mem_pestat] mean and std.dev: (2738.65, 2623.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15421) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (658, 1718, 4232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11380) [M::mem_pestat] mean and std.dev: (2763.23, 2661.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14954) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 271.089 CPU sec, 17.408 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3398, 91381, 3317, 3447) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (692, 1710, 4115) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10961) [M::mem_pestat] mean and std.dev: (2745.64, 2590.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14384) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 379, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860) [M::mem_pestat] mean and std.dev: (396.33, 137.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1046) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (638, 1755, 4350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11774) [M::mem_pestat] mean and std.dev: (2798.02, 2696.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15486) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (663, 1675, 4030) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10764) [M::mem_pestat] mean and std.dev: (2696.84, 2602.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14131) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 269.655 CPU sec, 17.263 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3428, 92937, 3264, 3379) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (683, 1754, 4468) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12038) [M::mem_pestat] mean and std.dev: (2851.43, 2710.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15823) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (301, 378, 486) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 856) [M::mem_pestat] mean and std.dev: (394.29, 135.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1041) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (638, 1677, 4316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11672) [M::mem_pestat] mean and std.dev: (2755.83, 2654.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15350) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (666, 1662, 4113) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11007) [M::mem_pestat] mean and std.dev: (2748.26, 2638.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14454) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 262.041 CPU sec, 16.606 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3537, 93385, 3204, 3609) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (638, 1684, 4247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11465) [M::mem_pestat] mean and std.dev: (2719.50, 2608.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15074) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (301, 377, 484) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 850) [M::mem_pestat] mean and std.dev: (392.77, 135.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1033) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (624, 1639, 4136) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11160) [M::mem_pestat] mean and std.dev: (2695.16, 2635.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14672) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (643, 1660, 4329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11701) [M::mem_pestat] mean and std.dev: (2769.86, 2643.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15387) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 263.699 CPU sec, 16.764 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3402, 92775, 3222, 3415) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (654, 1628, 4112) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11028) [M::mem_pestat] mean and std.dev: (2701.64, 2609.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14486) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (300, 378, 483) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 849) [M::mem_pestat] mean and std.dev: (392.73, 135.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1032) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (610, 1684, 4082) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11026) [M::mem_pestat] mean and std.dev: (2667.54, 2573.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14498) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (663, 1680, 4030) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10764) [M::mem_pestat] mean and std.dev: (2672.52, 2558.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14131) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 263.984 CPU sec, 16.872 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3315, 93293, 3287, 3280) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (639, 1686, 4173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11241) [M::mem_pestat] mean and std.dev: (2720.99, 2610.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14775) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 378, 486) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 854) [M::mem_pestat] mean and std.dev: (394.40, 135.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1038) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (654, 1714, 4015) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10737) [M::mem_pestat] mean and std.dev: (2739.30, 2668.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14098) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (654, 1732, 4271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11505) [M::mem_pestat] mean and std.dev: (2746.18, 2619.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15122) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 262.995 CPU sec, 16.659 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3499, 93001, 3434, 3499) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (637, 1652, 3949) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10573) [M::mem_pestat] mean and std.dev: (2655.85, 2548.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13885) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 378, 487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 857) [M::mem_pestat] mean and std.dev: (394.91, 136.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1042) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (583, 1591, 4037) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10945) [M::mem_pestat] mean and std.dev: (2629.82, 2571.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14399) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (653, 1716, 4132) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11090) [M::mem_pestat] mean and std.dev: (2748.31, 2647.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14569) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 263.229 CPU sec, 16.745 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3437, 91932, 3319, 3487) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (636, 1580, 3847) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10269) [M::mem_pestat] mean and std.dev: (2603.74, 2531.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13480) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (301, 378, 485) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 853) [M::mem_pestat] mean and std.dev: (394.38, 136.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1037) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (596, 1569, 4048) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10952) [M::mem_pestat] mean and std.dev: (2686.54, 2678.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14404) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (685, 1656, 4037) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10741) [M::mem_pestat] mean and std.dev: (2662.16, 2534.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14093) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 264.761 CPU sec, 16.974 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3485, 91995, 3190, 3481) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (622, 1663, 3868) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10360) [M::mem_pestat] mean and std.dev: (2652.24, 2568.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13606) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 378, 487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 857) [M::mem_pestat] mean and std.dev: (394.70, 136.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1042) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (599, 1705, 4247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11543) [M::mem_pestat] mean and std.dev: (2721.97, 2656.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15191) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (622, 1537, 3997) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10747) [M::mem_pestat] mean and std.dev: (2618.53, 2581.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14122) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 263.607 CPU sec, 16.844 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3430, 93720, 3231, 3391) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (646, 1635, 3999) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10705) [M::mem_pestat] mean and std.dev: (2665.21, 2565.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14058) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 379, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860) [M::mem_pestat] mean and std.dev: (396.11, 137.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1046) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (647, 1726, 4357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11777) [M::mem_pestat] mean and std.dev: (2806.76, 2697.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15487) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (672, 1740, 4199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11253) [M::mem_pestat] mean and std.dev: (2743.72, 2573.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14780) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 262.119 CPU sec, 16.883 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3493, 92518, 3320, 3429) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (649, 1629, 4042) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10828) [M::mem_pestat] mean and std.dev: (2703.27, 2599.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14221) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 379, 487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 857) [M::mem_pestat] mean and std.dev: (395.33, 136.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1042) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (624, 1708, 4314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11694) [M::mem_pestat] mean and std.dev: (2757.60, 2655.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15384) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (641, 1580, 4178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11252) [M::mem_pestat] mean and std.dev: (2713.33, 2628.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14789) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 268.074 CPU sec, 17.028 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3550, 92230, 3189, 3391) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (630, 1571, 4041) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10863) [M::mem_pestat] mean and std.dev: (2688.20, 2640.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14274) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 380, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 858) [M::mem_pestat] mean and std.dev: (396.25, 136.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1043) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (634, 1628, 4166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11230) [M::mem_pestat] mean and std.dev: (2717.13, 2628.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14762) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (671, 1662, 4218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11312) [M::mem_pestat] mean and std.dev: (2766.39, 2655.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14859) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 263.556 CPU sec, 16.800 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3478, 91948, 3191, 3390) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (643, 1615, 4157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11185) [M::mem_pestat] mean and std.dev: (2716.70, 2647.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14699) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 380, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861) [M::mem_pestat] mean and std.dev: (396.44, 136.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1047) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (625, 1713, 4145) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11185) [M::mem_pestat] mean and std.dev: (2709.45, 2606.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14705) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (634, 1551, 4178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11266) [M::mem_pestat] mean and std.dev: (2699.02, 2643.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14810) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 260.550 CPU sec, 16.564 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3314, 92635, 3189, 3483) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (636, 1600, 4193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11307) [M::mem_pestat] mean and std.dev: (2751.54, 2669.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14864) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 380, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861) [M::mem_pestat] mean and std.dev: (396.33, 137.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1047) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (623, 1618, 3986) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10712) [M::mem_pestat] mean and std.dev: (2672.47, 2639.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14075) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (615, 1633, 3963) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10659) [M::mem_pestat] mean and std.dev: (2660.45, 2597.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14007) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 263.944 CPU sec, 16.937 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3381, 91615, 3399, 3416) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (631, 1610, 4039) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10855) [M::mem_pestat] mean and std.dev: (2669.75, 2594.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14263) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 380, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860) [M::mem_pestat] mean and std.dev: (396.64, 136.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1046) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (600, 1747, 4373) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11919) [M::mem_pestat] mean and std.dev: (2783.61, 2683.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15692) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (613, 1521, 4035) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10879) [M::mem_pestat] mean and std.dev: (2639.04, 2578.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14301) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 263.538 CPU sec, 16.754 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3581, 91985, 3242, 3450) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (645, 1641, 4128) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11094) [M::mem_pestat] mean and std.dev: (2698.52, 2590.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14577) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 380, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861) [M::mem_pestat] mean and std.dev: (396.65, 137.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1047) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (585, 1595, 4135) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11235) [M::mem_pestat] mean and std.dev: (2667.48, 2636.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14785) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (665, 1673, 4092) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10946) [M::mem_pestat] mean and std.dev: (2706.04, 2601.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14373) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 262.068 CPU sec, 16.696 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3414, 92740, 3188, 3371) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (673, 1662, 4284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11506) [M::mem_pestat] mean and std.dev: (2746.09, 2646.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15117) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 381, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 867) [M::mem_pestat] mean and std.dev: (398.05, 138.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1055) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (595, 1582, 4171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11323) [M::mem_pestat] mean and std.dev: (2656.22, 2621.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14899) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (677, 1670, 4162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11132) [M::mem_pestat] mean and std.dev: (2729.24, 2613.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14617) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 261.359 CPU sec, 16.803 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3371, 92675, 3181, 3447) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (641, 1662, 4224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11390) [M::mem_pestat] mean and std.dev: (2721.01, 2617.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14973) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (304, 381, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 865) [M::mem_pestat] mean and std.dev: (397.21, 137.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1052) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (599, 1636, 3950) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10652) [M::mem_pestat] mean and std.dev: (2631.81, 2572.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14003) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (657, 1755, 4089) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10953) [M::mem_pestat] mean and std.dev: (2730.12, 2598.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14385) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 265.668 CPU sec, 17.157 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3501, 92410, 3346, 3439) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (645, 1664, 4361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11793) [M::mem_pestat] mean and std.dev: (2773.29, 2658.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15509) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 380, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 858) [M::mem_pestat] mean and std.dev: (396.64, 136.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1043) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (624, 1756, 4129) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11139) [M::mem_pestat] mean and std.dev: (2703.06, 2578.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14644) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (596, 1704, 4159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11285) [M::mem_pestat] mean and std.dev: (2736.44, 2631.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14848) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 263.669 CPU sec, 17.054 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3277, 92066, 3364, 3553) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (659, 1706, 4066) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10880) [M::mem_pestat] mean and std.dev: (2714.12, 2591.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14287) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (304, 381, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 862) [M::mem_pestat] mean and std.dev: (397.80, 137.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1048) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (633, 1708, 4156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11202) [M::mem_pestat] mean and std.dev: (2750.42, 2664.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14725) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (674, 1703, 4223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11321) [M::mem_pestat] mean and std.dev: (2719.10, 2577.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14870) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 263.061 CPU sec, 16.680 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3512, 92033, 3366, 3473) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (619, 1562, 4014) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10804) [M::mem_pestat] mean and std.dev: (2658.03, 2619.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14199) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 380, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861) [M::mem_pestat] mean and std.dev: (396.99, 137.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1047) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (612, 1631, 4160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11256) [M::mem_pestat] mean and std.dev: (2679.06, 2610.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14804) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (636, 1616, 4203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11337) [M::mem_pestat] mean and std.dev: (2705.32, 2617.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14904) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 263.984 CPU sec, 16.880 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3520, 92126, 3267, 3466) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (663, 1591, 4045) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10809) [M::mem_pestat] mean and std.dev: (2662.75, 2585.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14191) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 380, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864) [M::mem_pestat] mean and std.dev: (397.31, 137.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1051) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (625, 1713, 4186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11308) [M::mem_pestat] mean and std.dev: (2740.69, 2657.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14869) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (630, 1629, 4032) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10836) [M::mem_pestat] mean and std.dev: (2639.67, 2546.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14238) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 263.619 CPU sec, 16.731 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3535, 92451, 3322, 3555) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (634, 1737, 4119) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11089) [M::mem_pestat] mean and std.dev: (2721.17, 2578.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14574) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (304, 381, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 865) [M::mem_pestat] mean and std.dev: (397.66, 137.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1052) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (610, 1609, 4267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11581) [M::mem_pestat] mean and std.dev: (2694.84, 2643.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15238) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (637, 1754, 4256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11494) [M::mem_pestat] mean and std.dev: (2731.65, 2574.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15113) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 263.462 CPU sec, 16.820 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3461, 92383, 3178, 3496) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (659, 1609, 4101) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10985) [M::mem_pestat] mean and std.dev: (2701.87, 2602.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14427) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 380, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861) [M::mem_pestat] mean and std.dev: (397.03, 137.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1047) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (656, 1709, 4083) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10937) [M::mem_pestat] mean and std.dev: (2689.44, 2573.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14364) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (660, 1644, 4037) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10791) [M::mem_pestat] mean and std.dev: (2656.61, 2552.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14168) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 262.390 CPU sec, 16.801 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3403, 92327, 3267, 3332) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (625, 1610, 3956) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10618) [M::mem_pestat] mean and std.dev: (2661.56, 2606.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13949) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 381, 492) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 870) [M::mem_pestat] mean and std.dev: (398.19, 138.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1059) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (605, 1775, 4356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11858) [M::mem_pestat] mean and std.dev: (2787.41, 2666.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15609) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (650, 1661, 4053) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10859) [M::mem_pestat] mean and std.dev: (2674.08, 2571.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14262) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 264.105 CPU sec, 16.748 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3570, 91723, 3397, 3494) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (657, 1662, 4153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11145) [M::mem_pestat] mean and std.dev: (2752.85, 2667.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14641) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 380, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 858) [M::mem_pestat] mean and std.dev: (396.64, 136.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1043) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (625, 1624, 3877) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10381) [M::mem_pestat] mean and std.dev: (2632.01, 2590.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13633) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (649, 1720, 4354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11764) [M::mem_pestat] mean and std.dev: (2806.64, 2663.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15469) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 264.118 CPU sec, 16.970 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3554, 92028, 3351, 3443) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (652, 1704, 4110) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11026) [M::mem_pestat] mean and std.dev: (2700.15, 2584.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14484) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (304, 381, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 859) [M::mem_pestat] mean and std.dev: (397.76, 137.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1044) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (628, 1659, 4193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11323) [M::mem_pestat] mean and std.dev: (2728.03, 2661.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14888) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (626, 1576, 4162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11234) [M::mem_pestat] mean and std.dev: (2701.44, 2650.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14770) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 263.115 CPU sec, 16.686 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3422, 92272, 3323, 3454) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (666, 1706, 4211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11301) [M::mem_pestat] mean and std.dev: (2740.11, 2617.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14846) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 380, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861) [M::mem_pestat] mean and std.dev: (397.06, 137.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1047) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (659, 1764, 4095) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10967) [M::mem_pestat] mean and std.dev: (2763.07, 2658.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14403) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (619, 1600, 4208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11386) [M::mem_pestat] mean and std.dev: (2698.94, 2629.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14975) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 262.734 CPU sec, 16.811 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3389, 91960, 3352, 3525) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (653, 1623, 3998) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10688) [M::mem_pestat] mean and std.dev: (2671.03, 2588.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14033) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 380, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 863) [M::mem_pestat] mean and std.dev: (396.46, 137.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1050) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (609, 1611, 4115) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11127) [M::mem_pestat] mean and std.dev: (2673.46, 2625.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14633) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (620, 1674, 4219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11417) [M::mem_pestat] mean and std.dev: (2739.00, 2668.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15016) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 262.902 CPU sec, 16.842 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3473, 91726, 3432, 3425) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (646, 1539, 3945) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10543) [M::mem_pestat] mean and std.dev: (2672.11, 2628.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13842) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 381, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 867) [M::mem_pestat] mean and std.dev: (397.81, 138.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1055) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (635, 1751, 4460) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12110) [M::mem_pestat] mean and std.dev: (2803.99, 2661.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15935) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (641, 1695, 4312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11654) [M::mem_pestat] mean and std.dev: (2779.91, 2663.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15325) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 264.431 CPU sec, 16.774 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3482, 92009, 3378, 3410) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (652, 1641, 4061) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10879) [M::mem_pestat] mean and std.dev: (2696.44, 2588.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14288) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (304, 382, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 862) [M::mem_pestat] mean and std.dev: (397.93, 137.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1048) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (625, 1707, 4444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12082) [M::mem_pestat] mean and std.dev: (2792.56, 2713.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15901) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (663, 1705, 4176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11202) [M::mem_pestat] mean and std.dev: (2757.34, 2612.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14715) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 264.075 CPU sec, 16.912 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3356, 92473, 3360, 3483) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (626, 1655, 4040) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10868) [M::mem_pestat] mean and std.dev: (2668.24, 2580.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14282) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 381, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 867) [M::mem_pestat] mean and std.dev: (397.71, 137.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1055) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (622, 1727, 4227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11437) [M::mem_pestat] mean and std.dev: (2766.04, 2653.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15042) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (635, 1748, 4362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11816) [M::mem_pestat] mean and std.dev: (2752.11, 2604.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15543) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 263.329 CPU sec, 16.758 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3399, 91973, 3196, 3506) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (659, 1609, 3928) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10466) [M::mem_pestat] mean and std.dev: (2655.75, 2564.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13735) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 381, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864) [M::mem_pestat] mean and std.dev: (397.46, 137.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1051) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (646, 1751, 4354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11770) [M::mem_pestat] mean and std.dev: (2759.78, 2622.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15478) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (629, 1594, 4467) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12143) [M::mem_pestat] mean and std.dev: (2735.12, 2620.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15981) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 263.321 CPU sec, 17.038 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3454, 91730, 3316, 3331) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (650, 1672, 4009) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10727) [M::mem_pestat] mean and std.dev: (2698.63, 2577.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14086) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 381, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864) [M::mem_pestat] mean and std.dev: (397.65, 137.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1051) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (615, 1655, 4043) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10899) [M::mem_pestat] mean and std.dev: (2706.56, 2636.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14327) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (622, 1595, 4266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11554) [M::mem_pestat] mean and std.dev: (2710.80, 2639.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15198) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 263.209 CPU sec, 16.702 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3466, 91539, 3315, 3344) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (648, 1551, 3864) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10296) [M::mem_pestat] mean and std.dev: (2627.08, 2571.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13512) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 381, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 867) [M::mem_pestat] mean and std.dev: (398.05, 137.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1055) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (594, 1568, 4198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11406) [M::mem_pestat] mean and std.dev: (2690.64, 2668.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15010) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (637, 1601, 4071) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10939) [M::mem_pestat] mean and std.dev: (2664.10, 2598.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14373) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 265.342 CPU sec, 16.870 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3476, 92305, 3293, 3473) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (608, 1608, 3882) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10430) [M::mem_pestat] mean and std.dev: (2621.10, 2547.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13704) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 380, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 866) [M::mem_pestat] mean and std.dev: (396.86, 137.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1054) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (582, 1546, 4044) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10968) [M::mem_pestat] mean and std.dev: (2640.19, 2612.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14430) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (635, 1718, 4273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11549) [M::mem_pestat] mean and std.dev: (2725.15, 2580.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15187) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 264.004 CPU sec, 16.819 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3345, 92341, 3331, 3479) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (618, 1634, 4105) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11079) [M::mem_pestat] mean and std.dev: (2696.36, 2614.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14566) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 381, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 866) [M::mem_pestat] mean and std.dev: (398.14, 139.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1054) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (566, 1649, 4300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11768) [M::mem_pestat] mean and std.dev: (2694.00, 2638.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15502) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (676, 1690, 3996) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10636) [M::mem_pestat] mean and std.dev: (2691.78, 2546.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13956) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 264.510 CPU sec, 16.829 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3372, 91401, 3171, 3356) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (632, 1606, 3999) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10733) [M::mem_pestat] mean and std.dev: (2668.58, 2595.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14100) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 380, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864) [M::mem_pestat] mean and std.dev: (397.39, 138.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1051) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (640, 1696, 4072) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10936) [M::mem_pestat] mean and std.dev: (2677.57, 2606.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14368) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (625, 1603, 3803) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10159) [M::mem_pestat] mean and std.dev: (2586.69, 2517.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13337) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 263.216 CPU sec, 16.923 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3454, 91960, 3300, 3518) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (640, 1659, 4006) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10738) [M::mem_pestat] mean and std.dev: (2645.40, 2544.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14104) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 380, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861) [M::mem_pestat] mean and std.dev: (397.13, 138.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1047) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (595, 1602, 4314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11752) [M::mem_pestat] mean and std.dev: (2706.81, 2651.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15471) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (684, 1799, 4111) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10965) [M::mem_pestat] mean and std.dev: (2735.89, 2556.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14392) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 266.059 CPU sec, 16.849 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3351, 91978, 3270, 3416) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (655, 1709, 4181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11233) [M::mem_pestat] mean and std.dev: (2732.57, 2593.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14759) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 380, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 866) [M::mem_pestat] mean and std.dev: (397.26, 137.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1054) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (645, 1646, 3973) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10629) [M::mem_pestat] mean and std.dev: (2669.70, 2630.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13957) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (663, 1632, 3940) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10494) [M::mem_pestat] mean and std.dev: (2597.86, 2483.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13771) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 265.182 CPU sec, 16.994 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3402, 91795, 3322, 3565) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (678, 1687, 4190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11214) [M::mem_pestat] mean and std.dev: (2727.39, 2599.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14726) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 380, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 863) [M::mem_pestat] mean and std.dev: (397.23, 138.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1050) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (601, 1637, 4234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11500) [M::mem_pestat] mean and std.dev: (2712.54, 2650.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15133) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (616, 1580, 4028) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10852) [M::mem_pestat] mean and std.dev: (2641.33, 2591.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14264) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 266.496 CPU sec, 16.942 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3391, 92043, 3263, 3336) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (639, 1634, 4078) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10956) [M::mem_pestat] mean and std.dev: (2699.26, 2631.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14395) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 379, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860) [M::mem_pestat] mean and std.dev: (395.76, 136.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1046) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (621, 1683, 4345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11793) [M::mem_pestat] mean and std.dev: (2787.15, 2731.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15517) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (658, 1758, 4332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11680) [M::mem_pestat] mean and std.dev: (2789.18, 2651.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15354) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 268.440 CPU sec, 17.084 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3406, 92116, 3198, 3504) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (643, 1676, 3890) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10384) [M::mem_pestat] mean and std.dev: (2686.00, 2594.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13631) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 380, 486) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 852) [M::mem_pestat] mean and std.dev: (396.14, 135.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1035) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (612, 1620, 3998) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10770) [M::mem_pestat] mean and std.dev: (2661.59, 2605.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14156) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (613, 1661, 4105) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11089) [M::mem_pestat] mean and std.dev: (2685.53, 2618.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14581) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 265.798 CPU sec, 16.943 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3327, 92093, 3279, 3452) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (674, 1644, 4028) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10736) [M::mem_pestat] mean and std.dev: (2647.23, 2503.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14090) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 381, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 866) [M::mem_pestat] mean and std.dev: (397.66, 138.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1054) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (624, 1598, 4230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11442) [M::mem_pestat] mean and std.dev: (2732.27, 2670.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15048) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (627, 1611, 4039) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10863) [M::mem_pestat] mean and std.dev: (2653.42, 2591.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14275) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 268.180 CPU sec, 17.079 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3390, 91666, 3331, 3482) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (666, 1790, 4289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11535) [M::mem_pestat] mean and std.dev: (2812.82, 2670.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15158) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 380, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860) [M::mem_pestat] mean and std.dev: (396.80, 137.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1046) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (606, 1687, 4238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11502) [M::mem_pestat] mean and std.dev: (2731.28, 2655.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15134) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (655, 1663, 4306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11608) [M::mem_pestat] mean and std.dev: (2765.45, 2656.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15259) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 267.709 CPU sec, 17.084 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3474, 91956, 3218, 3441) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (667, 1675, 4167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11167) [M::mem_pestat] mean and std.dev: (2736.98, 2620.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14667) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 380, 487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 855) [M::mem_pestat] mean and std.dev: (396.48, 137.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1039) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (627, 1703, 4418) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12000) [M::mem_pestat] mean and std.dev: (2779.81, 2688.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15791) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (673, 1637, 3892) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10330) [M::mem_pestat] mean and std.dev: (2674.31, 2583.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13549) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 266.476 CPU sec, 16.990 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3482, 92009, 3257, 3273) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (634, 1640, 4090) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11002) [M::mem_pestat] mean and std.dev: (2673.10, 2577.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14458) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 380, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 863) [M::mem_pestat] mean and std.dev: (397.15, 138.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1050) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (613, 1667, 4151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11227) [M::mem_pestat] mean and std.dev: (2710.93, 2650.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14765) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (640, 1602, 4100) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11020) [M::mem_pestat] mean and std.dev: (2654.10, 2564.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14480) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 267.735 CPU sec, 17.054 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3468, 92315, 3300, 3429) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (660, 1668, 4146) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11118) [M::mem_pestat] mean and std.dev: (2707.98, 2585.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14604) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] (25, 50, 75) percentile: (302, 380, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 863) [M::mem_pestat] mean and std.dev: (397.39, 138.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1050) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (633, 1700, 4308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11658) [M::mem_pestat] mean and std.dev: (2736.41, 2631.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15333) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (658, 1657, 4219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11341) [M::mem_pestat] mean and std.dev: (2692.57, 2574.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14902) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 266.772 CPU sec, 17.086 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3305, 92110, 3158, 3338) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (663, 1651, 4055) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10839) [M::mem_pestat] mean and std.dev: (2727.74, 2633.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14231) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 380, 487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 855) [M::mem_pestat] mean and std.dev: (396.15, 136.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1039) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (601, 1663, 4238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11512) [M::mem_pestat] mean and std.dev: (2719.22, 2644.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15149) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (628, 1572, 4023) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10813) [M::mem_pestat] mean and std.dev: (2674.82, 2603.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14208) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 269.367 CPU sec, 17.306 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3444, 91733, 3283, 3508) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (650, 1630, 3972) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10616) [M::mem_pestat] mean and std.dev: (2646.47, 2566.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13938) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 380, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 863) [M::mem_pestat] mean and std.dev: (396.61, 137.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1050) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (634, 1696, 4364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11824) [M::mem_pestat] mean and std.dev: (2740.91, 2639.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15554) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (638, 1650, 4243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11453) [M::mem_pestat] mean and std.dev: (2710.30, 2608.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15058) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 268.080 CPU sec, 16.998 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3501, 92317, 3240, 3384) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (663, 1723, 4243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11403) [M::mem_pestat] mean and std.dev: (2756.78, 2651.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14983) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 379, 486) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 854) [M::mem_pestat] mean and std.dev: (395.55, 136.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1038) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (648, 1735, 4143) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11133) [M::mem_pestat] mean and std.dev: (2716.39, 2587.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14628) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (676, 1635, 4193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11227) [M::mem_pestat] mean and std.dev: (2738.56, 2658.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14744) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 265.512 CPU sec, 16.874 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3530, 92211, 3204, 3494) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (689, 1659, 4085) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10877) [M::mem_pestat] mean and std.dev: (2739.15, 2622.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14273) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (303, 378, 486) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 852) [M::mem_pestat] mean and std.dev: (395.35, 136.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1035) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (599, 1668, 4119) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11159) [M::mem_pestat] mean and std.dev: (2654.49, 2551.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14679) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (662, 1671, 4052) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10832) [M::mem_pestat] mean and std.dev: (2655.82, 2523.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14222) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 270.502 CPU sec, 17.875 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3299, 91474, 3202, 3434) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (655, 1524, 3781) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10033) [M::mem_pestat] mean and std.dev: (2552.79, 2492.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13159) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 379, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 863) [M::mem_pestat] mean and std.dev: (396.57, 138.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1050) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (594, 1606, 4191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11385) [M::mem_pestat] mean and std.dev: (2701.52, 2670.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14982) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (691, 1669, 3944) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10450) [M::mem_pestat] mean and std.dev: (2679.04, 2559.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13703) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 266.997 CPU sec, 16.920 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3426, 91631, 3363, 3533) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (669, 1709, 4090) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10932) [M::mem_pestat] mean and std.dev: (2742.15, 2628.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14353) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 379, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860) [M::mem_pestat] mean and std.dev: (396.08, 137.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1046) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (603, 1576, 4091) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11067) [M::mem_pestat] mean and std.dev: (2665.10, 2635.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14555) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (605, 1584, 4093) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11069) [M::mem_pestat] mean and std.dev: (2656.04, 2601.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14557) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 268.236 CPU sec, 17.204 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3516, 92318, 3151, 3406) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (645, 1641, 3782) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10056) [M::mem_pestat] mean and std.dev: (2611.22, 2513.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13193) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (301, 378, 486) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 856) [M::mem_pestat] mean and std.dev: (394.84, 136.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1041) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (602, 1644, 4198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11390) [M::mem_pestat] mean and std.dev: (2695.26, 2617.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14986) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (647, 1658, 4182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11252) [M::mem_pestat] mean and std.dev: (2698.29, 2606.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14787) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 269.070 CPU sec, 17.059 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3438, 92160, 3245, 3348) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (635, 1598, 3993) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10709) [M::mem_pestat] mean and std.dev: (2660.96, 2596.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14067) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 379, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860) [M::mem_pestat] mean and std.dev: (395.95, 136.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1046) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (642, 1615, 4185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11271) [M::mem_pestat] mean and std.dev: (2691.85, 2626.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14814) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (660, 1690, 4290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11550) [M::mem_pestat] mean and std.dev: (2758.09, 2638.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15180) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 268.130 CPU sec, 17.121 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3313, 92336, 3265, 3376) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (610, 1625, 4018) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10834) [M::mem_pestat] mean and std.dev: (2658.47, 2593.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14242) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (301, 378, 487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 859) [M::mem_pestat] mean and std.dev: (395.50, 137.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1045) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (593, 1570, 3899) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10511) [M::mem_pestat] mean and std.dev: (2598.50, 2570.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13817) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (658, 1592, 3953) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10543) [M::mem_pestat] mean and std.dev: (2663.38, 2588.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13838) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 266.501 CPU sec, 17.163 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3424, 91715, 3258, 3387) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (683, 1628, 4092) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10910) [M::mem_pestat] mean and std.dev: (2687.39, 2596.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14319) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 379, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860) [M::mem_pestat] mean and std.dev: (396.02, 137.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1046) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (610, 1710, 4333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11779) [M::mem_pestat] mean and std.dev: (2741.82, 2631.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15502) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (620, 1609, 4114) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11102) [M::mem_pestat] mean and std.dev: (2687.55, 2642.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14596) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 266.906 CPU sec, 16.910 real sec [M::process] read 1324504 sequences (200000104 bp)... [M::process] read 1324504 sequences (200000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3400, 92224, 3296, 3359) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (640, 1614, 3965) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10615) [M::mem_pestat] mean and std.dev: (2674.85, 2598.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13940) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 379, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860) [M::mem_pestat] mean and std.dev: (395.93, 137.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1046) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (595, 1661, 4003) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10819) [M::mem_pestat] mean and std.dev: (2667.32, 2632.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14227) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (673, 1718, 4220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11314) [M::mem_pestat] mean and std.dev: (2747.08, 2581.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14861) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 267.061 CPU sec, 17.164 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3310, 92294, 3242, 3504) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (641, 1746, 4168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11222) [M::mem_pestat] mean and std.dev: (2743.23, 2607.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14749) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 379, 487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 857) [M::mem_pestat] mean and std.dev: (395.65, 136.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1042) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (618, 1704, 4264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11556) [M::mem_pestat] mean and std.dev: (2742.55, 2636.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15202) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (630, 1560, 3982) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10686) [M::mem_pestat] mean and std.dev: (2624.20, 2570.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14038) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1324504 sequences (200000104 bp)... [M::mem_process_seqs] Processed 1324504 reads in 268.957 CPU sec, 17.052 real sec [M::process] read 968940 sequences (146309940 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3437, 91416, 3179, 3263) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (636, 1605, 4016) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10776) [M::mem_pestat] mean and std.dev: (2669.13, 2603.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14156) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 379, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 863) [M::mem_pestat] mean and std.dev: (396.64, 138.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1050) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (597, 1637, 4325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11781) [M::mem_pestat] mean and std.dev: (2727.11, 2650.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15509) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (678, 1709, 4142) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11070) [M::mem_pestat] mean and std.dev: (2747.56, 2616.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14534) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1324504 reads in 265.645 CPU sec, 16.965 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2483, 67037, 2410, 2469) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (658, 1700, 4276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11512) [M::mem_pestat] mean and std.dev: (2736.98, 2594.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15130) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (302, 379, 487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 857) [M::mem_pestat] mean and std.dev: (395.83, 137.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1042) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (606, 1598, 3859) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10365) [M::mem_pestat] mean and std.dev: (2600.42, 2564.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13618) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (671, 1819, 4221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11321) [M::mem_pestat] mean and std.dev: (2784.44, 2607.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14871) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 968940 reads in 196.325 CPU sec, 12.635 real sec [main] Version: 0.7.17-r1188 [main] CMD: bwa mem -SP5M -t 20 /oak/stanford/groups/akundaje/marinovg/papers/2024_ToL_Hi-C/2023-07-09/05-Diatom-Epithemia_sp._CRS-2021b/hifiasm.hic.p_ctg.fa /oak/stanford/groups/akundaje/marinovg/papers/2024_ToL_Hi-C/2023-07-09/05-Diatom-Epithemia_sp._CRS-2021b/juicer-hifiasm.hic.p_ctg/splits/reads_R1.fastq.gz /oak/stanford/groups/akundaje/marinovg/papers/2024_ToL_Hi-C/2023-07-09/05-Diatom-Epithemia_sp._CRS-2021b/juicer-hifiasm.hic.p_ctg/splits/reads_R2.fastq.gz [main] Real time: 12363.734 sec; CPU: 192075.770 sec Picked up _JAVA_OPTIONS: -Xmx16384m Picked up _JAVA_OPTIONS: -Xmx16384m Picked up _JAVA_OPTIONS: -Xmx16384m Picked up _JAVA_OPTIONS: -Xmx16384m