[M::bwa_idx_load_from_disk] read 0 ALT contigs [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2105, 388915, 1588, 2157) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (325, 1536, 4313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12289) [M::mem_pestat] mean and std.dev: (2681.11, 2805.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16277) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (97, 143, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 451) [M::mem_pestat] mean and std.dev: (160.59, 86.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 569) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (677, 2315, 5272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14462) [M::mem_pestat] mean and std.dev: (3208.60, 2890.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19057) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (262, 1162, 3697) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10567) [M::mem_pestat] mean and std.dev: (2341.20, 2659.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14002) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1932.451 CPU sec, 179.784 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2277, 389149, 1684, 2235) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (374, 1480, 4393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12431) [M::mem_pestat] mean and std.dev: (2684.90, 2787.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16450) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 148, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (166.74, 91.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 599) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (664, 2220, 5413) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14911) [M::mem_pestat] mean and std.dev: (3199.60, 2939.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19660) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (322, 1516, 4317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12307) [M::mem_pestat] mean and std.dev: (2625.90, 2798.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16302) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1604.304 CPU sec, 103.015 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2311, 389924, 1751, 2268) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (378, 1645, 4642) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13170) [M::mem_pestat] mean and std.dev: (2768.38, 2835.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17434) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (100, 149, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (167.86, 92.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 604) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (710, 2353, 5259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14357) [M::mem_pestat] mean and std.dev: (3246.47, 2916.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18906) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (305, 1348, 4241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12113) [M::mem_pestat] mean and std.dev: (2572.02, 2757.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16049) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1676.591 CPU sec, 106.266 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2279, 391135, 1783, 2410) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (341, 1563, 4348) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12362) [M::mem_pestat] mean and std.dev: (2682.30, 2802.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16369) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (100, 149, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (168.10, 92.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 604) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (697, 2329, 5333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14605) [M::mem_pestat] mean and std.dev: (3246.75, 2899.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19241) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (312, 1323, 4288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12240) [M::mem_pestat] mean and std.dev: (2611.73, 2838.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16216) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1630.260 CPU sec, 102.261 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2247, 388837, 1784, 2385) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (341, 1442, 4526) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12896) [M::mem_pestat] mean and std.dev: (2713.63, 2863.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17081) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (100, 149, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (168.71, 93.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 608) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (699, 2231, 5430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14892) [M::mem_pestat] mean and std.dev: (3227.26, 2932.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19623) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (324, 1380, 4274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12174) [M::mem_pestat] mean and std.dev: (2621.59, 2812.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16124) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1647.466 CPU sec, 103.429 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2252, 392386, 1806, 2413) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (305, 1354, 4149) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11837) [M::mem_pestat] mean and std.dev: (2544.82, 2766.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15681) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (100, 149, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (169.15, 93.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 612) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (674, 2372, 5331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14645) [M::mem_pestat] mean and std.dev: (3223.09, 2890.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19302) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (322, 1446, 4387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12517) [M::mem_pestat] mean and std.dev: (2638.49, 2821.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16582) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1609.615 CPU sec, 100.954 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2281, 393156, 1750, 2312) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (355, 1616, 4620) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13150) [M::mem_pestat] mean and std.dev: (2766.94, 2853.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17415) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (100, 149, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (168.80, 92.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 608) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (607, 2176, 5110) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14116) [M::mem_pestat] mean and std.dev: (3123.94, 2872.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18619) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (323, 1397, 4510) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12884) [M::mem_pestat] mean and std.dev: (2679.40, 2864.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17071) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1596.875 CPU sec, 100.155 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2278, 395780, 1721, 2365) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (331, 1406, 4291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12211) [M::mem_pestat] mean and std.dev: (2605.34, 2787.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16171) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (100, 148, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (166.76, 90.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 596) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (748, 2349, 5290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14374) [M::mem_pestat] mean and std.dev: (3213.86, 2882.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18916) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (279, 1346, 4491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12915) [M::mem_pestat] mean and std.dev: (2610.24, 2805.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17127) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1568.567 CPU sec, 98.722 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2244, 395225, 1748, 2393) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (334, 1474, 4612) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13168) [M::mem_pestat] mean and std.dev: (2678.18, 2817.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17446) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (100, 148, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (167.23, 91.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 600) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (677, 2312, 5102) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13952) [M::mem_pestat] mean and std.dev: (3125.17, 2823.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18377) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (280, 1235, 4287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12301) [M::mem_pestat] mean and std.dev: (2525.37, 2787.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16308) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1589.249 CPU sec, 99.685 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2260, 393789, 1704, 2399) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (335, 1455, 4463) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12719) [M::mem_pestat] mean and std.dev: (2717.89, 2847.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16847) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (100, 149, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (167.87, 92.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 604) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (683, 2348, 5409) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14861) [M::mem_pestat] mean and std.dev: (3203.23, 2861.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19587) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (301, 1420, 4295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12283) [M::mem_pestat] mean and std.dev: (2589.25, 2787.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16277) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1615.887 CPU sec, 101.345 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2339, 396377, 1823, 2392) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (322, 1428, 4459) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12733) [M::mem_pestat] mean and std.dev: (2619.53, 2772.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16870) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (100, 149, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (168.11, 92.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 604) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (652, 2284, 5175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14221) [M::mem_pestat] mean and std.dev: (3187.94, 2898.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18744) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (310, 1330, 4427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12661) [M::mem_pestat] mean and std.dev: (2642.67, 2865.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16778) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1602.681 CPU sec, 100.497 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2320, 397916, 1706, 2376) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (339, 1521, 4619) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13179) [M::mem_pestat] mean and std.dev: (2753.99, 2853.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17459) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (100, 148, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (166.57, 90.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 596) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (663, 2299, 5321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14637) [M::mem_pestat] mean and std.dev: (3230.61, 2927.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19295) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (308, 1305, 4063) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11573) [M::mem_pestat] mean and std.dev: (2525.05, 2723.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15328) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1616.248 CPU sec, 102.323 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2231, 390382, 1720, 2323) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (334, 1422, 4386) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12490) [M::mem_pestat] mean and std.dev: (2629.63, 2794.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16542) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (100, 148, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (167.76, 92.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 604) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (728, 2669, 5660) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15524) [M::mem_pestat] mean and std.dev: (3384.29, 2915.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20456) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (327, 1527, 4464) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12738) [M::mem_pestat] mean and std.dev: (2698.39, 2843.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16875) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1640.396 CPU sec, 102.998 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2260, 390273, 1758, 2439) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (334, 1492, 4045) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11467) [M::mem_pestat] mean and std.dev: (2571.97, 2743.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15178) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (100, 149, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (168.40, 93.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 608) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (680, 2372, 5524) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15212) [M::mem_pestat] mean and std.dev: (3294.85, 2938.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20056) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (305, 1305, 4226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12068) [M::mem_pestat] mean and std.dev: (2604.88, 2845.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15989) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1621.821 CPU sec, 101.740 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2266, 390532, 1740, 2325) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (354, 1521, 4359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12369) [M::mem_pestat] mean and std.dev: (2722.32, 2887.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16374) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (100, 149, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (168.44, 93.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 608) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (734, 2441, 5241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14255) [M::mem_pestat] mean and std.dev: (3208.88, 2799.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18762) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (329, 1461, 4511) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12875) [M::mem_pestat] mean and std.dev: (2682.46, 2842.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17057) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1620.294 CPU sec, 101.666 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2209, 389317, 1629, 2186) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (339, 1465, 4426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12600) [M::mem_pestat] mean and std.dev: (2673.83, 2817.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16687) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 147, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (166.02, 91.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 599) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (667, 2343, 5201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14269) [M::mem_pestat] mean and std.dev: (3175.46, 2881.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18803) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (310, 1252, 4096) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11668) [M::mem_pestat] mean and std.dev: (2548.98, 2788.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15454) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1783.324 CPU sec, 112.850 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2255, 393084, 1804, 2405) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (363, 1537, 4909) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14001) [M::mem_pestat] mean and std.dev: (2810.85, 2894.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18547) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (100, 148, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (167.74, 92.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 604) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (598, 2268, 5324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14776) [M::mem_pestat] mean and std.dev: (3175.69, 2909.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19502) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (343, 1449, 4223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11983) [M::mem_pestat] mean and std.dev: (2641.83, 2814.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15863) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1602.630 CPU sec, 100.511 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2357, 394732, 1813, 2301) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (349, 1466, 4271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12115) [M::mem_pestat] mean and std.dev: (2619.12, 2775.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16037) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 148, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (167.54, 92.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 603) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (666, 2314, 5517) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15219) [M::mem_pestat] mean and std.dev: (3266.20, 2945.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20070) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (322, 1530, 4491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12829) [M::mem_pestat] mean and std.dev: (2740.76, 2881.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16998) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1591.152 CPU sec, 99.760 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2237, 393876, 1767, 2296) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (345, 1425, 4423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12579) [M::mem_pestat] mean and std.dev: (2672.18, 2818.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16657) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (100, 148, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (167.14, 91.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 600) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (709, 2247, 5346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14620) [M::mem_pestat] mean and std.dev: (3195.74, 2874.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19257) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (304, 1397, 4350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12442) [M::mem_pestat] mean and std.dev: (2611.04, 2813.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16488) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1621.723 CPU sec, 101.724 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2371, 393055, 1746, 2273) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (300, 1342, 4429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12687) [M::mem_pestat] mean and std.dev: (2634.78, 2832.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16816) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 147, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (166.58, 91.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 599) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (657, 2227, 5383) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14835) [M::mem_pestat] mean and std.dev: (3199.83, 2930.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19561) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (294, 1345, 4460) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12792) [M::mem_pestat] mean and std.dev: (2634.87, 2822.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16958) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1684.873 CPU sec, 105.668 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2242, 392411, 1770, 2343) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (363, 1428, 4517) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12825) [M::mem_pestat] mean and std.dev: (2662.44, 2804.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16979) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 148, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (167.17, 92.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 603) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (705, 2406, 5467) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14991) [M::mem_pestat] mean and std.dev: (3277.50, 2922.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19753) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (327, 1499, 4593) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13125) [M::mem_pestat] mean and std.dev: (2712.29, 2823.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17391) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1695.548 CPU sec, 107.054 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2127, 391089, 1631, 2186) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (314, 1424, 4402) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12578) [M::mem_pestat] mean and std.dev: (2650.05, 2809.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16666) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (97, 143, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 454) [M::mem_pestat] mean and std.dev: (161.34, 87.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 573) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (682, 2301, 5436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14944) [M::mem_pestat] mean and std.dev: (3220.88, 2916.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19698) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (299, 1380, 4423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12671) [M::mem_pestat] mean and std.dev: (2630.79, 2817.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16795) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1973.691 CPU sec, 130.371 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2233, 386043, 1649, 2102) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (340, 1495, 4669) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13327) [M::mem_pestat] mean and std.dev: (2772.03, 2887.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17656) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (98, 145, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 467) [M::mem_pestat] mean and std.dev: (164.33, 90.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (632, 2364, 5084) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13988) [M::mem_pestat] mean and std.dev: (3163.08, 2845.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18440) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (269, 1261, 3998) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11456) [M::mem_pestat] mean and std.dev: (2458.40, 2747.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15185) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1901.825 CPU sec, 119.720 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2266, 385717, 1711, 2378) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (320, 1474, 4674) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13382) [M::mem_pestat] mean and std.dev: (2708.65, 2837.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17736) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 147, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (166.75, 92.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 603) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (677, 2325, 5480) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15086) [M::mem_pestat] mean and std.dev: (3252.95, 2949.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19889) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (318, 1515, 4587) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13125) [M::mem_pestat] mean and std.dev: (2686.15, 2829.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17394) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1786.700 CPU sec, 112.225 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2126, 385447, 1706, 2432) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (363, 1498, 4109) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11601) [M::mem_pestat] mean and std.dev: (2632.61, 2795.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15347) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 148, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 483) [M::mem_pestat] mean and std.dev: (168.28, 93.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 611) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (584, 2197, 5326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14810) [M::mem_pestat] mean and std.dev: (3189.27, 2932.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19552) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (314, 1311, 4308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12296) [M::mem_pestat] mean and std.dev: (2610.77, 2823.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16290) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1766.801 CPU sec, 110.750 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2283, 387403, 1800, 2373) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (344, 1640, 4547) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12953) [M::mem_pestat] mean and std.dev: (2743.52, 2815.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17156) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (100, 148, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (168.49, 93.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 608) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (599, 2336, 5151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14255) [M::mem_pestat] mean and std.dev: (3117.93, 2823.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18807) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (307, 1456, 4418) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12640) [M::mem_pestat] mean and std.dev: (2641.29, 2798.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16751) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1665.882 CPU sec, 104.546 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2282, 389211, 1772, 2361) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (334, 1531, 4615) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13177) [M::mem_pestat] mean and std.dev: (2750.17, 2852.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17458) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (100, 148, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (168.59, 93.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 608) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (749, 2466, 5439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14819) [M::mem_pestat] mean and std.dev: (3349.84, 2947.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19509) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (301, 1350, 4279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12235) [M::mem_pestat] mean and std.dev: (2635.01, 2837.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16213) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1619.175 CPU sec, 101.529 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2325, 389628, 1822, 2394) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (355, 1647, 4529) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12877) [M::mem_pestat] mean and std.dev: (2724.39, 2791.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17051) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (100, 148, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (167.88, 92.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 604) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (655, 2272, 5383) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14839) [M::mem_pestat] mean and std.dev: (3253.26, 2945.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19567) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (307, 1426, 4352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12442) [M::mem_pestat] mean and std.dev: (2614.41, 2798.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16487) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1656.448 CPU sec, 103.914 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2281, 389209, 1685, 2250) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (338, 1522, 4503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12833) [M::mem_pestat] mean and std.dev: (2717.40, 2842.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16998) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (100, 148, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (167.96, 92.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 604) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (716, 2494, 5228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14252) [M::mem_pestat] mean and std.dev: (3249.10, 2856.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18764) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (293, 1286, 4223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12083) [M::mem_pestat] mean and std.dev: (2582.18, 2817.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16013) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1695.766 CPU sec, 106.332 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2264, 389873, 1779, 2480) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (375, 1586, 4661) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13233) [M::mem_pestat] mean and std.dev: (2785.55, 2875.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17519) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (100, 149, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (168.73, 93.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 612) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (741, 2531, 5268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14322) [M::mem_pestat] mean and std.dev: (3244.00, 2835.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18849) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (278, 1319, 4400) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12644) [M::mem_pestat] mean and std.dev: (2611.99, 2840.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16766) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1660.475 CPU sec, 104.506 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2260, 393610, 1800, 2263) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (329, 1499, 4389) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12509) [M::mem_pestat] mean and std.dev: (2667.87, 2785.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16569) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (100, 148, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (166.57, 91.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 596) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (719, 2386, 5461) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14945) [M::mem_pestat] mean and std.dev: (3265.56, 2879.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19687) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (288, 1358, 4184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11976) [M::mem_pestat] mean and std.dev: (2532.77, 2735.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15872) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1648.441 CPU sec, 103.375 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2329, 395010, 1792, 2334) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (331, 1450, 4454) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12700) [M::mem_pestat] mean and std.dev: (2646.78, 2794.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16823) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (100, 148, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (166.59, 90.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 596) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (680, 2332, 5269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14447) [M::mem_pestat] mean and std.dev: (3163.97, 2837.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19036) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (300, 1359, 4230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12090) [M::mem_pestat] mean and std.dev: (2592.93, 2784.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16020) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1604.340 CPU sec, 100.628 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2236, 394006, 1822, 2373) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (340, 1536, 4332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12316) [M::mem_pestat] mean and std.dev: (2662.59, 2772.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16308) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 148, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (167.02, 91.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 603) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (717, 2373, 5407) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14787) [M::mem_pestat] mean and std.dev: (3272.79, 2927.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19477) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (372, 1571, 4641) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13179) [M::mem_pestat] mean and std.dev: (2739.76, 2831.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17448) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1615.725 CPU sec, 101.328 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2243, 390031, 1769, 2279) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (356, 1604, 4474) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12710) [M::mem_pestat] mean and std.dev: (2738.38, 2836.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16828) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 148, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480) [M::mem_pestat] mean and std.dev: (167.55, 92.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 607) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (711, 2417, 5270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14388) [M::mem_pestat] mean and std.dev: (3262.36, 2874.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18947) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (309, 1477, 4495) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12867) [M::mem_pestat] mean and std.dev: (2693.14, 2860.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17053) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1705.221 CPU sec, 106.959 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2289, 396497, 1662, 2281) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (339, 1464, 4681) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13365) [M::mem_pestat] mean and std.dev: (2733.69, 2874.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17707) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 148, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (166.96, 91.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 603) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (721, 2426, 5594) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15340) [M::mem_pestat] mean and std.dev: (3321.63, 2948.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20213) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (310, 1325, 4298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12274) [M::mem_pestat] mean and std.dev: (2640.23, 2879.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16262) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1629.337 CPU sec, 102.189 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2256, 394976, 1704, 2259) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (329, 1475, 4517) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12893) [M::mem_pestat] mean and std.dev: (2677.61, 2816.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17081) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (98, 146, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 464) [M::mem_pestat] mean and std.dev: (163.99, 89.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (759, 2680, 5435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14787) [M::mem_pestat] mean and std.dev: (3348.91, 2907.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19463) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (304, 1388, 4442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12718) [M::mem_pestat] mean and std.dev: (2666.23, 2868.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16856) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1837.607 CPU sec, 117.740 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2099, 388626, 1552, 2177) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (330, 1594, 4671) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13353) [M::mem_pestat] mean and std.dev: (2783.70, 2896.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17694) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (97, 143, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 454) [M::mem_pestat] mean and std.dev: (160.78, 86.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 573) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (644, 2349, 5321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14675) [M::mem_pestat] mean and std.dev: (3196.88, 2922.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19352) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (299, 1289, 4198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11996) [M::mem_pestat] mean and std.dev: (2574.54, 2799.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15895) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1960.814 CPU sec, 123.009 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2195, 387317, 1669, 2226) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (324, 1381, 4514) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12894) [M::mem_pestat] mean and std.dev: (2644.65, 2810.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17084) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 146, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (165.62, 91.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 595) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (651, 2307, 5282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14544) [M::mem_pestat] mean and std.dev: (3181.51, 2889.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19175) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (312, 1537, 4832) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13872) [M::mem_pestat] mean and std.dev: (2765.32, 2879.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18392) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1809.605 CPU sec, 113.475 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2260, 389200, 1732, 2330) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (305, 1355, 4104) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11702) [M::mem_pestat] mean and std.dev: (2599.27, 2807.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15501) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 147, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (165.70, 90.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 595) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (664, 2343, 5190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14242) [M::mem_pestat] mean and std.dev: (3131.00, 2811.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18768) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (272, 1264, 3978) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11390) [M::mem_pestat] mean and std.dev: (2500.35, 2768.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15096) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1771.031 CPU sec, 111.037 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2266, 389807, 1761, 2238) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (341, 1396, 4483) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12767) [M::mem_pestat] mean and std.dev: (2648.88, 2798.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16909) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (100, 148, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (167.12, 91.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 600) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (576, 2420, 5426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15126) [M::mem_pestat] mean and std.dev: (3248.00, 2962.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19976) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (279, 1264, 4453) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12801) [M::mem_pestat] mean and std.dev: (2585.64, 2851.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16975) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1699.897 CPU sec, 106.630 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2207, 391253, 1799, 2336) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (332, 1501, 4441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12659) [M::mem_pestat] mean and std.dev: (2720.17, 2854.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16768) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (100, 149, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (167.98, 92.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 604) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (727, 2379, 5387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14707) [M::mem_pestat] mean and std.dev: (3279.82, 2935.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19367) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (319, 1420, 4410) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12592) [M::mem_pestat] mean and std.dev: (2680.09, 2845.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16683) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1638.241 CPU sec, 102.816 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2257, 392758, 1739, 2340) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (371, 1645, 4639) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13175) [M::mem_pestat] mean and std.dev: (2808.21, 2865.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17443) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (100, 148, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (167.44, 91.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 600) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (693, 2384, 5580) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15354) [M::mem_pestat] mean and std.dev: (3319.24, 2989.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20241) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (287, 1297, 4207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12047) [M::mem_pestat] mean and std.dev: (2555.53, 2808.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15967) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1634.965 CPU sec, 102.532 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2398, 388604, 1783, 2408) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (360, 1585, 4600) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13080) [M::mem_pestat] mean and std.dev: (2714.41, 2774.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17320) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (100, 149, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (169.22, 93.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 612) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (683, 2492, 5515) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15179) [M::mem_pestat] mean and std.dev: (3282.73, 2915.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20011) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (306, 1442, 4686) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13446) [M::mem_pestat] mean and std.dev: (2716.51, 2886.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17826) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1647.716 CPU sec, 103.319 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2325, 391930, 1853, 2394) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (320, 1329, 4406) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12578) [M::mem_pestat] mean and std.dev: (2632.94, 2839.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16664) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (100, 149, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (168.12, 91.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 604) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (726, 2568, 5542) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15174) [M::mem_pestat] mean and std.dev: (3353.74, 2927.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19990) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (287, 1463, 4385) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12581) [M::mem_pestat] mean and std.dev: (2648.91, 2825.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16679) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1614.288 CPU sec, 101.248 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2201, 394887, 1776, 2271) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (324, 1417, 4334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12354) [M::mem_pestat] mean and std.dev: (2676.91, 2852.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16364) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (100, 148, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (167.90, 92.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 604) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (680, 2330, 5340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14660) [M::mem_pestat] mean and std.dev: (3215.23, 2905.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19320) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (297, 1151, 3896) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11094) [M::mem_pestat] mean and std.dev: (2440.56, 2720.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14693) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1586.955 CPU sec, 99.574 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2339, 395272, 1760, 2351) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (336, 1424, 4584) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13080) [M::mem_pestat] mean and std.dev: (2732.89, 2911.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17328) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (100, 148, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (167.25, 91.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 600) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (674, 2430, 5414) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14894) [M::mem_pestat] mean and std.dev: (3279.26, 2942.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19634) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (299, 1423, 4397) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12593) [M::mem_pestat] mean and std.dev: (2645.09, 2825.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16691) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1589.902 CPU sec, 99.711 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2315, 392255, 1887, 2350) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (322, 1566, 4543) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12985) [M::mem_pestat] mean and std.dev: (2699.93, 2812.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17206) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (100, 149, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (168.30, 92.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 604) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (624, 2390, 5451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15105) [M::mem_pestat] mean and std.dev: (3253.42, 2929.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19932) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (305, 1313, 4356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12458) [M::mem_pestat] mean and std.dev: (2589.45, 2819.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16509) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1632.415 CPU sec, 102.425 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2275, 395281, 1729, 2284) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (329, 1463, 4494) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12824) [M::mem_pestat] mean and std.dev: (2702.82, 2873.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16989) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (100, 148, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (167.53, 91.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 600) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (580, 2304, 5412) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15076) [M::mem_pestat] mean and std.dev: (3148.92, 2888.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19908) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (331, 1447, 4367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12439) [M::mem_pestat] mean and std.dev: (2665.46, 2812.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16475) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1591.909 CPU sec, 99.846 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2268, 397686, 1753, 2367) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (368, 1668, 4730) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13454) [M::mem_pestat] mean and std.dev: (2817.18, 2877.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17816) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (100, 148, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (167.47, 91.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 600) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (695, 2187, 5217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14261) [M::mem_pestat] mean and std.dev: (3169.26, 2896.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18783) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (326, 1355, 4384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12500) [M::mem_pestat] mean and std.dev: (2616.66, 2789.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16558) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1597.376 CPU sec, 100.321 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2057, 394839, 1566, 2102) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (338, 1503, 4583) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13073) [M::mem_pestat] mean and std.dev: (2711.24, 2844.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17318) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (98, 144, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 452) [M::mem_pestat] mean and std.dev: (161.30, 86.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 570) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (704, 2492, 5337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14603) [M::mem_pestat] mean and std.dev: (3250.29, 2842.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19236) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (314, 1542, 4494) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12854) [M::mem_pestat] mean and std.dev: (2693.50, 2843.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17034) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1853.735 CPU sec, 116.323 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2107, 388262, 1622, 2223) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (328, 1419, 4405) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12559) [M::mem_pestat] mean and std.dev: (2653.66, 2818.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16636) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (98, 144, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 458) [M::mem_pestat] mean and std.dev: (162.24, 87.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 578) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (674, 2402, 5556) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15320) [M::mem_pestat] mean and std.dev: (3321.83, 2990.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20202) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (327, 1405, 4537) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12957) [M::mem_pestat] mean and std.dev: (2668.83, 2816.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17167) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1840.144 CPU sec, 115.859 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2352, 418438, 1746, 2424) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (309, 1336, 4279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12219) [M::mem_pestat] mean and std.dev: (2547.71, 2731.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16189) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (94, 137, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 427) [M::mem_pestat] mean and std.dev: (153.27, 80.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 538) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (554, 2332, 5289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14759) [M::mem_pestat] mean and std.dev: (3172.37, 2925.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19494) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (287, 1436, 4555) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13091) [M::mem_pestat] mean and std.dev: (2684.80, 2853.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17359) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1172.109 CPU sec, 73.924 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2623, 431920, 2116, 2670) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (314, 1283, 4670) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13382) [M::mem_pestat] mean and std.dev: (2671.42, 2881.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17738) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (93, 134, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (149.37, 77.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 521) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (591, 2141, 5308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14742) [M::mem_pestat] mean and std.dev: (3144.86, 2908.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19459) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (285, 1360, 4350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12480) [M::mem_pestat] mean and std.dev: (2579.42, 2776.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16545) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 619.420 CPU sec, 39.038 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2670, 429125, 2071, 2700) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (302, 1288, 4168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11900) [M::mem_pestat] mean and std.dev: (2544.18, 2772.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15766) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (94, 136, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421) [M::mem_pestat] mean and std.dev: (151.21, 78.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (632, 2357, 5452) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15092) [M::mem_pestat] mean and std.dev: (3255.35, 2966.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19912) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (294, 1390, 4328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12396) [M::mem_pestat] mean and std.dev: (2621.50, 2834.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16430) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 636.634 CPU sec, 40.081 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2630, 430122, 2004, 2754) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (308, 1381, 4332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12380) [M::mem_pestat] mean and std.dev: (2605.84, 2828.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16404) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (94, 135, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418) [M::mem_pestat] mean and std.dev: (150.69, 78.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 526) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (617, 2170, 5091) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14039) [M::mem_pestat] mean and std.dev: (3057.10, 2819.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18513) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (308, 1365, 4486) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12842) [M::mem_pestat] mean and std.dev: (2657.22, 2832.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17020) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 623.454 CPU sec, 39.256 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2749, 430025, 2090, 2776) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (319, 1297, 3998) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11356) [M::mem_pestat] mean and std.dev: (2504.70, 2716.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15035) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (94, 136, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421) [M::mem_pestat] mean and std.dev: (151.54, 78.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (561, 1925, 4845) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13413) [M::mem_pestat] mean and std.dev: (2976.04, 2872.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17697) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (297, 1367, 4560) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13086) [M::mem_pestat] mean and std.dev: (2653.39, 2845.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17349) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 611.915 CPU sec, 38.580 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2677, 427472, 2164, 2820) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (327, 1382, 4307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12267) [M::mem_pestat] mean and std.dev: (2633.03, 2807.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16247) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (94, 136, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 424) [M::mem_pestat] mean and std.dev: (152.03, 79.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 534) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (698, 2343, 5325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14579) [M::mem_pestat] mean and std.dev: (3218.37, 2870.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19206) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (311, 1340, 4079) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11615) [M::mem_pestat] mean and std.dev: (2563.31, 2789.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15383) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 613.809 CPU sec, 38.707 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2804, 429487, 2020, 2710) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (321, 1391, 4360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12438) [M::mem_pestat] mean and std.dev: (2643.22, 2817.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16477) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (94, 136, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 424) [M::mem_pestat] mean and std.dev: (151.98, 79.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 534) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (613, 2428, 5525) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15349) [M::mem_pestat] mean and std.dev: (3330.35, 3018.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20261) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (274, 1407, 4148) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11896) [M::mem_pestat] mean and std.dev: (2547.29, 2768.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15770) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 606.056 CPU sec, 38.190 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2672, 429318, 2109, 2688) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (305, 1384, 4214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12032) [M::mem_pestat] mean and std.dev: (2560.67, 2734.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15941) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (94, 136, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 424) [M::mem_pestat] mean and std.dev: (152.06, 79.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 534) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (582, 2258, 5317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14787) [M::mem_pestat] mean and std.dev: (3185.23, 2941.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19522) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (306, 1309, 4145) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11823) [M::mem_pestat] mean and std.dev: (2637.01, 2875.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15662) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 597.311 CPU sec, 37.621 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2735, 432659, 2111, 2751) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (308, 1404, 4381) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12527) [M::mem_pestat] mean and std.dev: (2639.50, 2841.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16600) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (94, 135, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418) [M::mem_pestat] mean and std.dev: (150.92, 78.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 526) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (664, 2304, 5269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14479) [M::mem_pestat] mean and std.dev: (3213.84, 2928.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19084) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (332, 1387, 4243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12065) [M::mem_pestat] mean and std.dev: (2602.46, 2769.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15976) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 604.672 CPU sec, 38.126 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2579, 434607, 2088, 2708) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (329, 1388, 4231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12035) [M::mem_pestat] mean and std.dev: (2586.50, 2753.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15937) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (93, 135, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 417) [M::mem_pestat] mean and std.dev: (150.27, 77.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 525) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (642, 2247, 5212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14352) [M::mem_pestat] mean and std.dev: (3163.77, 2903.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18922) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (270, 1278, 4180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12000) [M::mem_pestat] mean and std.dev: (2524.35, 2773.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15910) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 588.125 CPU sec, 37.180 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2598, 430497, 2015, 2771) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (328, 1387, 4267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12145) [M::mem_pestat] mean and std.dev: (2632.23, 2863.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16084) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (94, 135, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418) [M::mem_pestat] mean and std.dev: (150.82, 78.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 526) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (551, 2085, 4851) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13451) [M::mem_pestat] mean and std.dev: (2964.68, 2787.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17751) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (296, 1350, 4271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12221) [M::mem_pestat] mean and std.dev: (2575.51, 2790.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16196) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 608.806 CPU sec, 38.453 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2687, 432886, 2047, 2845) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (324, 1501, 4452) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12708) [M::mem_pestat] mean and std.dev: (2704.22, 2860.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16836) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (94, 135, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418) [M::mem_pestat] mean and std.dev: (150.76, 77.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 526) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (616, 2195, 4994) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13750) [M::mem_pestat] mean and std.dev: (3073.26, 2832.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18128) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (306, 1485, 4409) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12615) [M::mem_pestat] mean and std.dev: (2648.60, 2786.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16718) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 582.412 CPU sec, 36.753 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2688, 433821, 2162, 2733) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (311, 1303, 4102) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11684) [M::mem_pestat] mean and std.dev: (2532.20, 2750.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15475) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (94, 136, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418) [M::mem_pestat] mean and std.dev: (151.04, 78.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 526) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (592, 2179, 5087) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14077) [M::mem_pestat] mean and std.dev: (3105.24, 2892.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18572) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (318, 1432, 4387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12525) [M::mem_pestat] mean and std.dev: (2637.39, 2786.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16594) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 597.938 CPU sec, 37.688 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2580, 436560, 1998, 2698) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (310, 1447, 4434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12682) [M::mem_pestat] mean and std.dev: (2625.81, 2796.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16806) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (94, 135, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 415) [M::mem_pestat] mean and std.dev: (150.19, 77.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 522) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (690, 2324, 5376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14748) [M::mem_pestat] mean and std.dev: (3260.49, 2937.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19434) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (278, 1325, 4057) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11615) [M::mem_pestat] mean and std.dev: (2505.85, 2740.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15394) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 600.279 CPU sec, 37.832 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2812, 429252, 2075, 2752) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (317, 1529, 4272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12182) [M::mem_pestat] mean and std.dev: (2643.58, 2790.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16137) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (94, 136, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421) [M::mem_pestat] mean and std.dev: (151.15, 78.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (712, 2358, 5539) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15193) [M::mem_pestat] mean and std.dev: (3271.41, 2945.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20020) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (312, 1475, 4524) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12948) [M::mem_pestat] mean and std.dev: (2714.83, 2889.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17160) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 613.074 CPU sec, 38.648 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2628, 428206, 2125, 2689) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (323, 1489, 4430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12644) [M::mem_pestat] mean and std.dev: (2672.91, 2836.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16751) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (94, 136, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 424) [M::mem_pestat] mean and std.dev: (151.83, 79.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 534) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (653, 2471, 5646) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15632) [M::mem_pestat] mean and std.dev: (3308.65, 2960.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20625) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (290, 1388, 4336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12428) [M::mem_pestat] mean and std.dev: (2608.73, 2815.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16474) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 610.178 CPU sec, 38.513 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2647, 427030, 2068, 2743) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (316, 1313, 4338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12382) [M::mem_pestat] mean and std.dev: (2605.96, 2829.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16404) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (94, 136, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 424) [M::mem_pestat] mean and std.dev: (151.90, 79.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 534) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (612, 2272, 5240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14496) [M::mem_pestat] mean and std.dev: (3149.71, 2853.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19124) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (307, 1375, 4213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12025) [M::mem_pestat] mean and std.dev: (2520.19, 2691.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15931) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 606.096 CPU sec, 38.375 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2681, 428121, 2079, 2753) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (309, 1378, 4371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12495) [M::mem_pestat] mean and std.dev: (2626.15, 2822.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16557) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (94, 136, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421) [M::mem_pestat] mean and std.dev: (151.18, 78.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (627, 2261, 5319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14703) [M::mem_pestat] mean and std.dev: (3187.80, 2910.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19395) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (300, 1278, 4275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12225) [M::mem_pestat] mean and std.dev: (2571.08, 2804.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16200) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 625.573 CPU sec, 39.480 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2673, 431637, 2025, 2749) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (332, 1544, 4477) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12767) [M::mem_pestat] mean and std.dev: (2697.71, 2811.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16912) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (93, 134, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (149.56, 77.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 521) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (574, 2295, 5080) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14092) [M::mem_pestat] mean and std.dev: (3157.26, 2883.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18598) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (306, 1495, 4358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12462) [M::mem_pestat] mean and std.dev: (2662.73, 2833.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16514) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 653.221 CPU sec, 41.144 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2735, 431175, 2050, 2723) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (335, 1452, 4405) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12545) [M::mem_pestat] mean and std.dev: (2676.80, 2848.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16615) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (94, 135, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418) [M::mem_pestat] mean and std.dev: (150.71, 78.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 526) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (634, 2259, 5063) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13921) [M::mem_pestat] mean and std.dev: (3089.48, 2836.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18350) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (313, 1380, 4429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12661) [M::mem_pestat] mean and std.dev: (2648.03, 2839.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16777) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 601.015 CPU sec, 37.954 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2698, 432378, 2146, 2614) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (340, 1389, 4157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11791) [M::mem_pestat] mean and std.dev: (2572.27, 2754.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15608) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (93, 135, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 420) [M::mem_pestat] mean and std.dev: (150.69, 78.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 529) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (541, 2064, 5021) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13981) [M::mem_pestat] mean and std.dev: (3038.06, 2885.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18461) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (295, 1253, 4019) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11467) [M::mem_pestat] mean and std.dev: (2488.73, 2727.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15191) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 606.827 CPU sec, 38.246 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2778, 433833, 2084, 2741) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (356, 1568, 4466) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12686) [M::mem_pestat] mean and std.dev: (2693.01, 2817.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16796) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (93, 135, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 417) [M::mem_pestat] mean and std.dev: (150.40, 78.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 525) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (601, 2211, 5224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14470) [M::mem_pestat] mean and std.dev: (3150.15, 2910.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19093) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (282, 1293, 4268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12240) [M::mem_pestat] mean and std.dev: (2589.74, 2847.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16226) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 591.905 CPU sec, 37.337 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2674, 432420, 2089, 2739) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (317, 1436, 4465) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12761) [M::mem_pestat] mean and std.dev: (2667.64, 2829.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16909) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (93, 135, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 420) [M::mem_pestat] mean and std.dev: (150.81, 78.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 529) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (670, 2417, 5296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14548) [M::mem_pestat] mean and std.dev: (3226.40, 2878.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19174) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (301, 1241, 4011) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11431) [M::mem_pestat] mean and std.dev: (2461.45, 2686.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15141) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 597.805 CPU sec, 37.669 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2654, 431268, 2024, 2711) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (321, 1368, 4244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12090) [M::mem_pestat] mean and std.dev: (2591.62, 2774.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16013) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (93, 135, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 420) [M::mem_pestat] mean and std.dev: (150.44, 78.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 529) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (627, 2301, 5192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14322) [M::mem_pestat] mean and std.dev: (3172.19, 2903.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18887) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (294, 1400, 4307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12333) [M::mem_pestat] mean and std.dev: (2586.41, 2754.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16346) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 664.327 CPU sec, 41.818 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2635, 434765, 2058, 2614) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (315, 1314, 4225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12045) [M::mem_pestat] mean and std.dev: (2607.00, 2846.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15955) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (93, 134, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411) [M::mem_pestat] mean and std.dev: (148.56, 76.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 517) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (621, 2223, 5278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14592) [M::mem_pestat] mean and std.dev: (3158.17, 2917.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19249) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (296, 1308, 4226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12086) [M::mem_pestat] mean and std.dev: (2546.74, 2750.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16016) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 688.884 CPU sec, 43.372 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2513, 437568, 1899, 2562) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (283, 1265, 4069) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11641) [M::mem_pestat] mean and std.dev: (2537.39, 2784.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15427) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (92, 132, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 404) [M::mem_pestat] mean and std.dev: (146.83, 75.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 508) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (657, 2375, 5426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14964) [M::mem_pestat] mean and std.dev: (3256.47, 2954.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19733) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (272, 1329, 4227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12137) [M::mem_pestat] mean and std.dev: (2535.02, 2756.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16092) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 731.311 CPU sec, 46.022 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2611, 430268, 1999, 2710) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (331, 1397, 4312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12274) [M::mem_pestat] mean and std.dev: (2613.00, 2790.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16255) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (93, 134, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 417) [M::mem_pestat] mean and std.dev: (149.82, 78.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 525) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (607, 2359, 5282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14632) [M::mem_pestat] mean and std.dev: (3174.55, 2876.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19307) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (273, 1226, 4094) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11736) [M::mem_pestat] mean and std.dev: (2507.82, 2790.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15557) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 675.152 CPU sec, 42.560 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2771, 426602, 1990, 2754) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (340, 1504, 4326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12298) [M::mem_pestat] mean and std.dev: (2643.77, 2788.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16284) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (93, 136, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 426) [M::mem_pestat] mean and std.dev: (151.91, 80.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 537) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (656, 2227, 5402) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14894) [M::mem_pestat] mean and std.dev: (3239.63, 2957.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19640) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (296, 1324, 4173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11927) [M::mem_pestat] mean and std.dev: (2573.29, 2793.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15804) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 642.038 CPU sec, 41.024 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2622, 427380, 2127, 2711) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (335, 1519, 4333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12329) [M::mem_pestat] mean and std.dev: (2670.65, 2813.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16327) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (93, 135, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 423) [M::mem_pestat] mean and std.dev: (151.13, 79.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 533) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (582, 2058, 4860) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13416) [M::mem_pestat] mean and std.dev: (3003.70, 2826.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17694) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (328, 1323, 4204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11956) [M::mem_pestat] mean and std.dev: (2564.68, 2744.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15832) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 652.516 CPU sec, 41.105 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2591, 427256, 2022, 2840) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (334, 1431, 4402) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12538) [M::mem_pestat] mean and std.dev: (2651.29, 2805.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16606) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (94, 136, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421) [M::mem_pestat] mean and std.dev: (151.36, 79.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (535, 2017, 4940) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13750) [M::mem_pestat] mean and std.dev: (2989.34, 2864.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18155) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (286, 1300, 4455) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12793) [M::mem_pestat] mean and std.dev: (2630.47, 2862.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16962) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 617.044 CPU sec, 38.970 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2678, 428006, 2082, 2697) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (312, 1510, 4671) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13389) [M::mem_pestat] mean and std.dev: (2738.82, 2881.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17748) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (94, 136, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 424) [M::mem_pestat] mean and std.dev: (152.01, 79.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 534) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (624, 2154, 5361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14835) [M::mem_pestat] mean and std.dev: (3150.11, 2904.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19572) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (282, 1358, 4213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12075) [M::mem_pestat] mean and std.dev: (2575.61, 2818.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16006) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 606.408 CPU sec, 38.209 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2663, 428006, 2148, 2705) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (345, 1414, 4425) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12585) [M::mem_pestat] mean and std.dev: (2671.40, 2837.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16665) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (94, 136, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 424) [M::mem_pestat] mean and std.dev: (151.82, 79.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 534) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (670, 2266, 5360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14740) [M::mem_pestat] mean and std.dev: (3226.70, 2932.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19430) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (310, 1331, 4261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12163) [M::mem_pestat] mean and std.dev: (2604.37, 2808.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16114) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 613.536 CPU sec, 38.667 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2736, 428218, 2108, 2839) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (326, 1393, 4322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12314) [M::mem_pestat] mean and std.dev: (2615.66, 2783.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16310) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (94, 136, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 424) [M::mem_pestat] mean and std.dev: (151.82, 79.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 534) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (672, 2367, 5170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14166) [M::mem_pestat] mean and std.dev: (3177.73, 2842.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18664) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (312, 1391, 4356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12444) [M::mem_pestat] mean and std.dev: (2623.00, 2815.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16488) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 601.180 CPU sec, 37.904 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2673, 428302, 2169, 2777) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (330, 1451, 4397) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12531) [M::mem_pestat] mean and std.dev: (2651.84, 2823.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16598) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (93, 135, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 423) [M::mem_pestat] mean and std.dev: (151.22, 79.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 533) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (639, 2105, 5128) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14106) [M::mem_pestat] mean and std.dev: (3095.63, 2885.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18595) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (307, 1460, 4395) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12571) [M::mem_pestat] mean and std.dev: (2655.25, 2830.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16659) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 630.248 CPU sec, 39.700 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2680, 428862, 2078, 2730) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (343, 1457, 4495) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12799) [M::mem_pestat] mean and std.dev: (2694.69, 2846.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16951) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (94, 136, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421) [M::mem_pestat] mean and std.dev: (151.28, 78.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (638, 2308, 5328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14708) [M::mem_pestat] mean and std.dev: (3225.21, 2930.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19398) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (291, 1358, 4418) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12672) [M::mem_pestat] mean and std.dev: (2640.56, 2839.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16799) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 592.748 CPU sec, 37.400 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2689, 432301, 2066, 2775) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (327, 1507, 4611) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13179) [M::mem_pestat] mean and std.dev: (2733.16, 2868.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17463) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (93, 135, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 417) [M::mem_pestat] mean and std.dev: (150.30, 77.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 525) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (698, 2410, 5284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14456) [M::mem_pestat] mean and std.dev: (3219.38, 2867.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19042) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (283, 1259, 4206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12052) [M::mem_pestat] mean and std.dev: (2538.55, 2795.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15975) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 597.742 CPU sec, 37.714 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2728, 432378, 1995, 2681) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (306, 1384, 4475) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12813) [M::mem_pestat] mean and std.dev: (2641.25, 2833.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16982) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (93, 135, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 417) [M::mem_pestat] mean and std.dev: (150.30, 77.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 525) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (645, 2301, 5214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14352) [M::mem_pestat] mean and std.dev: (3201.36, 2895.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18921) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (316, 1446, 4355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12433) [M::mem_pestat] mean and std.dev: (2590.10, 2765.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16472) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 595.327 CPU sec, 37.523 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2655, 432018, 2108, 2723) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (317, 1390, 4057) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11537) [M::mem_pestat] mean and std.dev: (2544.34, 2737.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15277) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (93, 135, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 420) [M::mem_pestat] mean and std.dev: (150.71, 78.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 529) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (630, 2211, 5105) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14055) [M::mem_pestat] mean and std.dev: (3098.90, 2852.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18530) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (292, 1349, 4165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11911) [M::mem_pestat] mean and std.dev: (2541.59, 2748.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15784) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 593.682 CPU sec, 37.425 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2753, 429656, 2045, 2758) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (330, 1377, 4403) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12549) [M::mem_pestat] mean and std.dev: (2638.79, 2830.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16622) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (93, 135, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 420) [M::mem_pestat] mean and std.dev: (150.85, 78.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 529) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (605, 2372, 5543) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15419) [M::mem_pestat] mean and std.dev: (3259.33, 2954.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20357) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (281, 1239, 4259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12215) [M::mem_pestat] mean and std.dev: (2542.26, 2817.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16193) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 622.218 CPU sec, 39.222 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2705, 433923, 2125, 2684) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (310, 1460, 4255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12145) [M::mem_pestat] mean and std.dev: (2633.23, 2795.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16090) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (93, 135, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 420) [M::mem_pestat] mean and std.dev: (150.71, 78.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 529) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (549, 2130, 5296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14790) [M::mem_pestat] mean and std.dev: (3139.04, 2929.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19537) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (306, 1423, 4456) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12756) [M::mem_pestat] mean and std.dev: (2662.17, 2821.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16906) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 600.000 CPU sec, 37.801 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2679, 433003, 2145, 2722) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (355, 1587, 4582) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13036) [M::mem_pestat] mean and std.dev: (2732.43, 2829.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17263) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (93, 136, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 423) [M::mem_pestat] mean and std.dev: (151.17, 79.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 533) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (630, 2271, 5393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14919) [M::mem_pestat] mean and std.dev: (3204.67, 2938.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19682) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (298, 1379, 4286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12262) [M::mem_pestat] mean and std.dev: (2614.25, 2794.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16250) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 651.121 CPU sec, 40.999 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2434, 443785, 1962, 2506) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (307, 1368, 4236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12094) [M::mem_pestat] mean and std.dev: (2599.95, 2816.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16023) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (91, 130, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 391) [M::mem_pestat] mean and std.dev: (143.84, 72.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 491) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (578, 2012, 5138) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14258) [M::mem_pestat] mean and std.dev: (3075.04, 2917.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18818) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (302, 1388, 4351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12449) [M::mem_pestat] mean and std.dev: (2602.67, 2772.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16498) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 758.884 CPU sec, 47.768 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2576, 433561, 1932, 2688) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (307, 1415, 4466) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12784) [M::mem_pestat] mean and std.dev: (2613.42, 2806.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16943) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (92, 133, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 410) [M::mem_pestat] mean and std.dev: (148.22, 76.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 516) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (633, 2190, 5134) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14136) [M::mem_pestat] mean and std.dev: (3069.34, 2839.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18637) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (283, 1252, 4439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12751) [M::mem_pestat] mean and std.dev: (2598.65, 2821.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16907) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 688.317 CPU sec, 43.335 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2781, 429799, 2111, 2726) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (347, 1445, 4469) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12713) [M::mem_pestat] mean and std.dev: (2680.27, 2828.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16835) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (93, 135, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 420) [M::mem_pestat] mean and std.dev: (150.42, 78.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 529) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (664, 2413, 5287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14533) [M::mem_pestat] mean and std.dev: (3216.46, 2883.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19156) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (289, 1310, 4187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11983) [M::mem_pestat] mean and std.dev: (2539.35, 2754.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15881) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 644.709 CPU sec, 40.636 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2677, 431523, 2120, 2718) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (294, 1277, 4282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12258) [M::mem_pestat] mean and std.dev: (2568.37, 2822.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16246) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (93, 135, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 417) [M::mem_pestat] mean and std.dev: (150.14, 77.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 525) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (627, 2305, 5308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14670) [M::mem_pestat] mean and std.dev: (3177.67, 2857.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19351) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (328, 1412, 4149) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11791) [M::mem_pestat] mean and std.dev: (2572.96, 2747.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15612) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 632.360 CPU sec, 39.845 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2662, 430852, 2127, 2651) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (309, 1511, 4620) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13242) [M::mem_pestat] mean and std.dev: (2749.40, 2897.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17553) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (94, 135, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418) [M::mem_pestat] mean and std.dev: (150.79, 78.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 526) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (596, 2168, 5176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14336) [M::mem_pestat] mean and std.dev: (3106.06, 2850.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18916) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (300, 1380, 4163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11889) [M::mem_pestat] mean and std.dev: (2557.83, 2727.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15752) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 614.432 CPU sec, 38.720 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2696, 429559, 2032, 2661) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (299, 1360, 4270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12212) [M::mem_pestat] mean and std.dev: (2603.85, 2839.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16183) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (94, 136, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421) [M::mem_pestat] mean and std.dev: (151.31, 78.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (686, 2111, 5051) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13781) [M::mem_pestat] mean and std.dev: (3107.09, 2890.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18146) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (301, 1300, 4330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12388) [M::mem_pestat] mean and std.dev: (2603.70, 2807.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16417) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 594.456 CPU sec, 37.446 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2682, 432046, 2032, 2728) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (325, 1491, 4321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12313) [M::mem_pestat] mean and std.dev: (2629.94, 2783.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16309) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (94, 135, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421) [M::mem_pestat] mean and std.dev: (151.22, 78.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (671, 2201, 5106) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13976) [M::mem_pestat] mean and std.dev: (3149.57, 2857.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18411) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (305, 1378, 4451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12743) [M::mem_pestat] mean and std.dev: (2606.50, 2776.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16889) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 592.735 CPU sec, 37.349 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2820, 427046, 2145, 2773) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (334, 1369, 4215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11977) [M::mem_pestat] mean and std.dev: (2596.15, 2793.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15858) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (94, 136, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 424) [M::mem_pestat] mean and std.dev: (152.02, 79.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 534) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (744, 2320, 5218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14166) [M::mem_pestat] mean and std.dev: (3208.66, 2867.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18640) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (299, 1406, 4324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12374) [M::mem_pestat] mean and std.dev: (2612.20, 2812.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16399) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 600.709 CPU sec, 37.866 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2645, 430022, 2098, 2764) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (314, 1421, 4390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12542) [M::mem_pestat] mean and std.dev: (2628.77, 2808.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16618) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (94, 136, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 424) [M::mem_pestat] mean and std.dev: (151.89, 79.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 534) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (658, 2239, 5347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14725) [M::mem_pestat] mean and std.dev: (3220.92, 2945.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19414) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (324, 1424, 4414) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12594) [M::mem_pestat] mean and std.dev: (2616.70, 2798.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16684) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 596.086 CPU sec, 37.560 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2650, 430568, 2080, 2852) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (313, 1444, 4385) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12529) [M::mem_pestat] mean and std.dev: (2655.15, 2827.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16601) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (94, 136, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421) [M::mem_pestat] mean and std.dev: (151.63, 78.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (615, 2193, 5222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14436) [M::mem_pestat] mean and std.dev: (3141.89, 2913.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19043) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (310, 1464, 4244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12112) [M::mem_pestat] mean and std.dev: (2621.71, 2781.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16046) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 589.638 CPU sec, 37.190 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2718, 432653, 2043, 2957) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (322, 1425, 4783) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13705) [M::mem_pestat] mean and std.dev: (2724.70, 2895.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18166) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (94, 136, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418) [M::mem_pestat] mean and std.dev: (150.91, 78.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 526) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (616, 2283, 5242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14494) [M::mem_pestat] mean and std.dev: (3187.45, 2937.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19120) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (280, 1294, 4131) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11833) [M::mem_pestat] mean and std.dev: (2522.41, 2771.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15684) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 582.996 CPU sec, 36.870 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2739, 433078, 2093, 2669) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (336, 1372, 4519) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12885) [M::mem_pestat] mean and std.dev: (2655.71, 2857.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17068) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (94, 135, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418) [M::mem_pestat] mean and std.dev: (150.85, 78.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 526) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (625, 2101, 5105) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14065) [M::mem_pestat] mean and std.dev: (3091.76, 2868.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18545) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (265, 1213, 4206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12088) [M::mem_pestat] mean and std.dev: (2510.45, 2826.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16029) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 585.729 CPU sec, 37.317 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2700, 429520, 2116, 2753) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (313, 1366, 4413) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12613) [M::mem_pestat] mean and std.dev: (2610.19, 2790.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16713) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (94, 136, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 424) [M::mem_pestat] mean and std.dev: (151.79, 79.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 534) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (658, 2323, 5459) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15061) [M::mem_pestat] mean and std.dev: (3237.59, 2927.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19862) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (292, 1280, 4119) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11773) [M::mem_pestat] mean and std.dev: (2541.17, 2790.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15600) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 606.518 CPU sec, 38.315 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2751, 432963, 2096, 2784) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (296, 1362, 4495) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12893) [M::mem_pestat] mean and std.dev: (2648.27, 2830.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17092) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (94, 136, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418) [M::mem_pestat] mean and std.dev: (151.06, 78.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 526) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (645, 2382, 5146) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14148) [M::mem_pestat] mean and std.dev: (3159.78, 2896.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18649) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (307, 1427, 4199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11983) [M::mem_pestat] mean and std.dev: (2614.29, 2812.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15875) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 596.018 CPU sec, 37.581 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2639, 434818, 2122, 2732) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (301, 1309, 4098) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11692) [M::mem_pestat] mean and std.dev: (2518.92, 2724.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15489) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (94, 136, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418) [M::mem_pestat] mean and std.dev: (150.94, 78.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 526) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (635, 2231, 5234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14432) [M::mem_pestat] mean and std.dev: (3122.69, 2849.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19031) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (293, 1341, 4243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12143) [M::mem_pestat] mean and std.dev: (2576.81, 2806.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16093) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 592.489 CPU sec, 37.368 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2480, 442623, 1972, 2627) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (320, 1362, 4330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12350) [M::mem_pestat] mean and std.dev: (2614.41, 2805.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16360) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (92, 132, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 401) [M::mem_pestat] mean and std.dev: (146.56, 74.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 504) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (543, 2112, 5307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14835) [M::mem_pestat] mean and std.dev: (3123.36, 2944.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19599) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (280, 1315, 4400) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12640) [M::mem_pestat] mean and std.dev: (2627.96, 2866.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16760) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 693.926 CPU sec, 43.699 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2597, 436121, 1948, 2598) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (296, 1295, 4244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12140) [M::mem_pestat] mean and std.dev: (2555.60, 2804.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16088) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (92, 132, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 404) [M::mem_pestat] mean and std.dev: (146.79, 74.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 508) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (650, 2169, 5297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14591) [M::mem_pestat] mean and std.dev: (3167.50, 2924.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19238) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (260, 1238, 4082) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11726) [M::mem_pestat] mean and std.dev: (2491.04, 2748.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15548) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 718.653 CPU sec, 45.305 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2150, 445743, 1678, 2243) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (327, 1508, 4352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12402) [M::mem_pestat] mean and std.dev: (2632.01, 2756.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16427) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (102, 149, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (169.36, 92.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (603, 2219, 5469) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15201) [M::mem_pestat] mean and std.dev: (3217.93, 2985.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20067) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (312, 1381, 4320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12336) [M::mem_pestat] mean and std.dev: (2618.33, 2835.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16344) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 843.556 CPU sec, 53.068 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (485, 505325, 453, 449) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (448, 1686, 4783) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13453) [M::mem_pestat] mean and std.dev: (2873.01, 2832.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17788) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 203, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 644) [M::mem_pestat] mean and std.dev: (232.55, 121.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 812) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (544, 2106, 5398) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15106) [M::mem_pestat] mean and std.dev: (3096.55, 2913.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19960) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (433, 1927, 5118) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14488) [M::mem_pestat] mean and std.dev: (2942.35, 2857.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19173) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1383.380 CPU sec, 86.845 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (528, 508012, 430, 514) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (440, 2020, 5366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15218) [M::mem_pestat] mean and std.dev: (3146.84, 3066.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20144) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 208, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667) [M::mem_pestat] mean and std.dev: (238.76, 125.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 842) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (632, 2279, 5887) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16397) [M::mem_pestat] mean and std.dev: (3340.31, 3050.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21652) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (329, 1721, 5149) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14789) [M::mem_pestat] mean and std.dev: (2887.39, 2948.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19609) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1162.741 CPU sec, 73.066 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (462, 508713, 390, 533) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (420, 1961, 4902) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13866) [M::mem_pestat] mean and std.dev: (3027.67, 2956.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18348) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 210, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671) [M::mem_pestat] mean and std.dev: (240.61, 126.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (693, 2346, 5541) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15237) [M::mem_pestat] mean and std.dev: (3364.69, 3031.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20085) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (379, 1656, 4791) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13615) [M::mem_pestat] mean and std.dev: (2781.04, 2777.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18027) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1253.551 CPU sec, 78.696 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (530, 509611, 431, 560) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (546, 2007, 4859) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13485) [M::mem_pestat] mean and std.dev: (3036.47, 2834.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17798) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 209, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667) [M::mem_pestat] mean and std.dev: (238.75, 125.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 842) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (471, 2428, 5269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14865) [M::mem_pestat] mean and std.dev: (3090.01, 2861.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19663) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (423, 1769, 4795) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13539) [M::mem_pestat] mean and std.dev: (2859.74, 2853.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17911) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1248.491 CPU sec, 78.349 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (499, 509119, 404, 505) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (408, 1628, 4337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12195) [M::mem_pestat] mean and std.dev: (2787.51, 2839.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16124) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 211, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 677) [M::mem_pestat] mean and std.dev: (241.55, 127.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 855) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (678, 2642, 5607) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15465) [M::mem_pestat] mean and std.dev: (3303.09, 2924.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20394) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (383, 1749, 4734) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13436) [M::mem_pestat] mean and std.dev: (2830.94, 2903.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17787) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1222.651 CPU sec, 76.766 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (508, 508131, 434, 512) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (400, 1557, 4353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12259) [M::mem_pestat] mean and std.dev: (2755.24, 2895.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16212) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 211, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 680) [M::mem_pestat] mean and std.dev: (242.39, 128.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 859) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (614, 2101, 5173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14291) [M::mem_pestat] mean and std.dev: (3201.25, 3080.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18850) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (442, 1970, 4739) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13333) [M::mem_pestat] mean and std.dev: (2954.49, 2938.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17630) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1241.290 CPU sec, 77.890 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (482, 510617, 441, 490) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (428, 2039, 5120) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14504) [M::mem_pestat] mean and std.dev: (3007.46, 2868.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19196) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 211, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674) [M::mem_pestat] mean and std.dev: (240.91, 126.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (484, 2193, 5527) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15613) [M::mem_pestat] mean and std.dev: (3203.69, 3014.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20656) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (394, 1828, 4865) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13807) [M::mem_pestat] mean and std.dev: (2882.00, 2865.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18278) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1216.094 CPU sec, 76.539 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (539, 510065, 402, 521) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (377, 1497, 4905) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13961) [M::mem_pestat] mean and std.dev: (2843.83, 2973.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18489) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 211, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672) [M::mem_pestat] mean and std.dev: (241.02, 126.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 848) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (841, 2836, 5987) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16279) [M::mem_pestat] mean and std.dev: (3495.77, 2926.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21425) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (373, 1997, 5033) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14353) [M::mem_pestat] mean and std.dev: (3013.92, 3026.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19013) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1226.006 CPU sec, 76.955 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (493, 510416, 420, 536) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (513, 1619, 4727) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13155) [M::mem_pestat] mean and std.dev: (2853.80, 2867.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17369) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 208, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661) [M::mem_pestat] mean and std.dev: (237.69, 123.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 834) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (778, 2476, 5776) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15772) [M::mem_pestat] mean and std.dev: (3321.99, 2942.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20770) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (346, 1587, 4672) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13324) [M::mem_pestat] mean and std.dev: (2786.16, 2928.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17650) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1236.900 CPU sec, 78.507 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (517, 510680, 426, 515) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (430, 1891, 5403) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15349) [M::mem_pestat] mean and std.dev: (3026.39, 2965.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20322) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 206, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651) [M::mem_pestat] mean and std.dev: (235.22, 122.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 821) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (679, 2734, 6241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 17365) [M::mem_pestat] mean and std.dev: (3536.72, 3092.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 22927) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (318, 1407, 4575) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13089) [M::mem_pestat] mean and std.dev: (2734.75, 2942.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17346) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1271.568 CPU sec, 79.829 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (547, 509929, 472, 495) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (367, 1869, 4872) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13882) [M::mem_pestat] mean and std.dev: (2940.52, 2894.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18387) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 207, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652) [M::mem_pestat] mean and std.dev: (235.93, 122.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 822) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (516, 2235, 5162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14454) [M::mem_pestat] mean and std.dev: (3044.67, 2822.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19100) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (479, 2177, 5562) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15728) [M::mem_pestat] mean and std.dev: (3141.69, 2975.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20811) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1282.190 CPU sec, 80.527 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (514, 508151, 467, 503) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (412, 1681, 4392) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12352) [M::mem_pestat] mean and std.dev: (2847.34, 2852.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16332) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 209, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668) [M::mem_pestat] mean and std.dev: (239.62, 125.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 843) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (647, 2034, 5037) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13817) [M::mem_pestat] mean and std.dev: (2991.07, 2802.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18207) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (398, 1656, 5163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14693) [M::mem_pestat] mean and std.dev: (3001.89, 3032.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19458) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1269.860 CPU sec, 79.679 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (510, 511074, 418, 509) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (450, 1893, 5156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14568) [M::mem_pestat] mean and std.dev: (3084.37, 2994.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19274) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 207, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655) [M::mem_pestat] mean and std.dev: (236.24, 122.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 826) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (486, 1856, 5080) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14268) [M::mem_pestat] mean and std.dev: (3024.50, 2870.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18862) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (387, 1745, 4776) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13554) [M::mem_pestat] mean and std.dev: (2885.81, 2855.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17943) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1282.251 CPU sec, 80.620 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (513, 510925, 406, 544) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (408, 1779, 4720) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13344) [M::mem_pestat] mean and std.dev: (2828.17, 2858.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17656) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 207, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655) [M::mem_pestat] mean and std.dev: (235.98, 122.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 826) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (640, 2509, 5821) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16183) [M::mem_pestat] mean and std.dev: (3365.10, 2964.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21364) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (391, 1655, 4791) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13591) [M::mem_pestat] mean and std.dev: (2873.34, 2942.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17991) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1289.596 CPU sec, 80.975 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (480, 512083, 435, 509) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (401, 1654, 4737) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13409) [M::mem_pestat] mean and std.dev: (2804.40, 2874.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17745) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 205, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648) [M::mem_pestat] mean and std.dev: (233.96, 121.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 817) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (677, 2491, 5915) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16391) [M::mem_pestat] mean and std.dev: (3418.99, 3053.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21629) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (358, 1544, 4072) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11500) [M::mem_pestat] mean and std.dev: (2659.28, 2788.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15214) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1311.897 CPU sec, 82.343 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (508, 510263, 409, 478) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (403, 1659, 5106) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14512) [M::mem_pestat] mean and std.dev: (2942.67, 2975.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19215) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 207, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655) [M::mem_pestat] mean and std.dev: (236.11, 122.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 826) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (560, 2349, 5673) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15899) [M::mem_pestat] mean and std.dev: (3320.22, 3015.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21012) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (434, 1950, 5226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14810) [M::mem_pestat] mean and std.dev: (3040.72, 2990.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19602) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1333.695 CPU sec, 83.684 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (539, 507614, 431, 502) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (465, 1681, 4615) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12915) [M::mem_pestat] mean and std.dev: (2792.35, 2787.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17065) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 210, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 677) [M::mem_pestat] mean and std.dev: (241.52, 127.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 855) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (781, 2347, 5420) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14698) [M::mem_pestat] mean and std.dev: (3290.43, 2922.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19337) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (365, 1603, 4575) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12995) [M::mem_pestat] mean and std.dev: (2774.79, 2846.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17205) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1257.919 CPU sec, 78.931 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (511, 507931, 420, 531) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (419, 1732, 4555) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12827) [M::mem_pestat] mean and std.dev: (2830.41, 2881.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16963) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 211, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674) [M::mem_pestat] mean and std.dev: (241.23, 127.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (592, 2193, 5287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14677) [M::mem_pestat] mean and std.dev: (3122.76, 2849.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19372) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (353, 1357, 5013) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14333) [M::mem_pestat] mean and std.dev: (2798.55, 3020.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18993) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1243.156 CPU sec, 78.019 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (477, 508522, 463, 526) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (387, 1888, 4942) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14052) [M::mem_pestat] mean and std.dev: (2872.68, 2831.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18607) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 211, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 677) [M::mem_pestat] mean and std.dev: (241.84, 127.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 855) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (682, 2493, 5211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14269) [M::mem_pestat] mean and std.dev: (3273.79, 2882.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18798) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (463, 2124, 4900) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13774) [M::mem_pestat] mean and std.dev: (3047.71, 2843.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18211) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1240.667 CPU sec, 78.018 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (530, 507418, 431, 495) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (422, 2259, 5280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14996) [M::mem_pestat] mean and std.dev: (3070.39, 2886.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19854) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 210, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674) [M::mem_pestat] mean and std.dev: (240.99, 127.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (540, 2505, 5097) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14211) [M::mem_pestat] mean and std.dev: (3119.81, 2772.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18768) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (381, 1674, 4962) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14124) [M::mem_pestat] mean and std.dev: (2873.25, 2946.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18705) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1265.221 CPU sec, 79.818 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (511, 506455, 456, 481) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (482, 1853, 4994) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14018) [M::mem_pestat] mean and std.dev: (2952.12, 2875.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18530) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 206, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660) [M::mem_pestat] mean and std.dev: (236.87, 124.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 833) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (920, 2786, 5704) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15272) [M::mem_pestat] mean and std.dev: (3459.07, 2871.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20056) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (414, 1777, 4784) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13524) [M::mem_pestat] mean and std.dev: (2873.20, 2870.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17894) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1349.072 CPU sec, 84.709 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (490, 507670, 456, 499) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (379, 1952, 5201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14845) [M::mem_pestat] mean and std.dev: (3046.80, 2986.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19667) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 208, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661) [M::mem_pestat] mean and std.dev: (237.79, 124.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 834) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (696, 2335, 5339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14625) [M::mem_pestat] mean and std.dev: (3234.89, 2880.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19268) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (402, 1983, 5117) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14547) [M::mem_pestat] mean and std.dev: (3005.95, 2979.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19262) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1309.513 CPU sec, 82.203 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (486, 508895, 396, 484) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (381, 1844, 4522) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12804) [M::mem_pestat] mean and std.dev: (2835.80, 2853.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16945) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 208, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664) [M::mem_pestat] mean and std.dev: (238.04, 125.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 838) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (543, 2425, 5327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14895) [M::mem_pestat] mean and std.dev: (3222.60, 2872.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19679) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (402, 2065, 5034) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14298) [M::mem_pestat] mean and std.dev: (3001.38, 2914.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18930) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1279.903 CPU sec, 80.367 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (462, 508196, 437, 525) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (439, 1817, 5001) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14125) [M::mem_pestat] mean and std.dev: (2915.15, 2849.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18687) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 206, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657) [M::mem_pestat] mean and std.dev: (236.49, 123.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 829) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (598, 2482, 6081) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 17047) [M::mem_pestat] mean and std.dev: (3449.04, 3095.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 22530) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (441, 1692, 4907) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13839) [M::mem_pestat] mean and std.dev: (2925.39, 2985.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18305) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1306.600 CPU sec, 82.012 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (522, 508713, 421, 487) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (418, 1874, 4847) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13705) [M::mem_pestat] mean and std.dev: (2905.88, 2807.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18134) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 207, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657) [M::mem_pestat] mean and std.dev: (236.50, 123.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 829) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (455, 2353, 5679) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16127) [M::mem_pestat] mean and std.dev: (3193.30, 2926.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21351) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (402, 1822, 5102) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14502) [M::mem_pestat] mean and std.dev: (2987.98, 2998.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19202) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1313.441 CPU sec, 82.685 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (500, 509268, 386, 485) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (358, 1565, 4487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12745) [M::mem_pestat] mean and std.dev: (2728.00, 2847.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16874) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 208, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661) [M::mem_pestat] mean and std.dev: (237.81, 124.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 834) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (640, 2423, 5395) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14905) [M::mem_pestat] mean and std.dev: (3259.93, 2987.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19660) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (417, 1850, 5191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14739) [M::mem_pestat] mean and std.dev: (2932.17, 2885.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19513) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1308.016 CPU sec, 82.071 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (489, 506546, 428, 512) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (546, 1934, 5366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15006) [M::mem_pestat] mean and std.dev: (3134.59, 2949.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19826) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 207, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663) [M::mem_pestat] mean and std.dev: (237.51, 125.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 837) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (551, 2314, 5823) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16367) [M::mem_pestat] mean and std.dev: (3292.47, 3011.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21639) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (509, 1851, 4834) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13484) [M::mem_pestat] mean and std.dev: (2954.67, 2817.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17809) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1335.344 CPU sec, 83.765 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (548, 509526, 466, 520) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (472, 2698, 6030) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 17146) [M::mem_pestat] mean and std.dev: (3459.95, 3123.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 22704) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 208, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667) [M::mem_pestat] mean and std.dev: (238.64, 125.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 842) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (642, 2122, 5731) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15909) [M::mem_pestat] mean and std.dev: (3278.33, 2988.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20998) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (453, 1854, 4944) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13926) [M::mem_pestat] mean and std.dev: (2950.44, 2906.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18417) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1376.401 CPU sec, 86.317 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (448, 504550, 373, 468) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (379, 1753, 4509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12769) [M::mem_pestat] mean and std.dev: (2745.44, 2753.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16899) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 199, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 629) [M::mem_pestat] mean and std.dev: (228.02, 118.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 793) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (499, 1910, 5624) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15874) [M::mem_pestat] mean and std.dev: (3178.18, 3066.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20999) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (239, 1087, 4052) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11678) [M::mem_pestat] mean and std.dev: (2500.27, 2887.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15491) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1455.898 CPU sec, 91.376 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (450, 505208, 474, 519) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (347, 1915, 4733) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13505) [M::mem_pestat] mean and std.dev: (2851.29, 2801.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17891) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 207, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666) [M::mem_pestat] mean and std.dev: (238.20, 125.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 841) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (658, 2514, 5349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14731) [M::mem_pestat] mean and std.dev: (3227.05, 2900.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19422) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (445, 1598, 4443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12439) [M::mem_pestat] mean and std.dev: (2747.07, 2842.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16437) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1350.212 CPU sec, 84.721 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (528, 505736, 471, 483) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (433, 1789, 5236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14842) [M::mem_pestat] mean and std.dev: (3024.29, 3052.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19645) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 209, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673) [M::mem_pestat] mean and std.dev: (240.16, 126.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 850) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (644, 2312, 5683) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15761) [M::mem_pestat] mean and std.dev: (3278.34, 2958.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20800) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (320, 1511, 4600) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13160) [M::mem_pestat] mean and std.dev: (2711.98, 2824.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17440) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1312.809 CPU sec, 82.359 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (512, 505647, 411, 523) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (514, 1734, 5195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14557) [M::mem_pestat] mean and std.dev: (2994.44, 2935.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19238) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 211, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 683) [M::mem_pestat] mean and std.dev: (242.55, 128.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 863) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (760, 2753, 5944) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16312) [M::mem_pestat] mean and std.dev: (3501.96, 2987.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21496) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (392, 1745, 4543) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12845) [M::mem_pestat] mean and std.dev: (2784.32, 2769.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16996) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1280.568 CPU sec, 80.347 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (508, 506080, 430, 524) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (464, 1579, 4837) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13583) [M::mem_pestat] mean and std.dev: (2790.06, 2798.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17956) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 212, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 684) [M::mem_pestat] mean and std.dev: (243.76, 128.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 864) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (700, 2200, 5193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14179) [M::mem_pestat] mean and std.dev: (3172.06, 2841.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18672) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (396, 1850, 4921) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13971) [M::mem_pestat] mean and std.dev: (2892.24, 2847.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18496) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1254.022 CPU sec, 78.705 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (497, 507146, 465, 549) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (384, 1773, 5370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15342) [M::mem_pestat] mean and std.dev: (3033.21, 3025.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20328) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 211, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 677) [M::mem_pestat] mean and std.dev: (242.11, 127.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 855) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (690, 2142, 6021) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16683) [M::mem_pestat] mean and std.dev: (3385.36, 3118.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 22014) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (307, 1439, 4909) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14113) [M::mem_pestat] mean and std.dev: (2721.35, 2881.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18715) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1252.530 CPU sec, 78.650 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (484, 508359, 428, 511) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (521, 1806, 5163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14447) [M::mem_pestat] mean and std.dev: (3141.61, 3055.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19089) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 211, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 677) [M::mem_pestat] mean and std.dev: (241.70, 127.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 855) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (545, 1955, 4920) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13670) [M::mem_pestat] mean and std.dev: (2928.74, 2783.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18045) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (453, 1934, 5138) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14508) [M::mem_pestat] mean and std.dev: (3014.23, 2984.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19193) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1247.520 CPU sec, 78.360 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (473, 508255, 426, 513) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (501, 1902, 4522) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12564) [M::mem_pestat] mean and std.dev: (2901.27, 2835.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16585) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 210, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674) [M::mem_pestat] mean and std.dev: (241.33, 127.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (596, 2164, 5393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14987) [M::mem_pestat] mean and std.dev: (3126.14, 2935.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19784) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (299, 1444, 4754) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13664) [M::mem_pestat] mean and std.dev: (2771.12, 2928.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18119) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1260.838 CPU sec, 79.220 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (518, 508241, 408, 542) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (418, 1808, 4749) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13411) [M::mem_pestat] mean and std.dev: (2908.66, 2805.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17742) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 210, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674) [M::mem_pestat] mean and std.dev: (241.58, 127.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (655, 2149, 4972) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13606) [M::mem_pestat] mean and std.dev: (3072.92, 2856.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17923) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (399, 1865, 4820) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13662) [M::mem_pestat] mean and std.dev: (2924.31, 2919.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18083) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1247.526 CPU sec, 78.442 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (496, 505899, 453, 515) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (534, 1969, 4930) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13722) [M::mem_pestat] mean and std.dev: (2981.86, 2872.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18118) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 210, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674) [M::mem_pestat] mean and std.dev: (241.03, 127.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (378, 1829, 4979) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14181) [M::mem_pestat] mean and std.dev: (2852.95, 2848.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18782) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (385, 1748, 5043) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14359) [M::mem_pestat] mean and std.dev: (2853.43, 2883.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19017) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1286.581 CPU sec, 80.768 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (500, 509895, 449, 519) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (483, 1783, 5260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14814) [M::mem_pestat] mean and std.dev: (3082.43, 3040.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19591) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 211, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675) [M::mem_pestat] mean and std.dev: (241.98, 127.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 852) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (673, 2319, 5304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14566) [M::mem_pestat] mean and std.dev: (3226.20, 2937.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19197) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (348, 1387, 4335) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12309) [M::mem_pestat] mean and std.dev: (2607.15, 2762.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16296) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1234.922 CPU sec, 77.586 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (514, 508134, 440, 527) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (422, 1789, 4953) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14015) [M::mem_pestat] mean and std.dev: (2935.89, 2932.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18546) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 207, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661) [M::mem_pestat] mean and std.dev: (237.80, 124.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 834) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (533, 2039, 5421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15197) [M::mem_pestat] mean and std.dev: (3214.11, 3073.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20085) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (418, 1621, 4447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12505) [M::mem_pestat] mean and std.dev: (2697.45, 2739.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16534) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1284.652 CPU sec, 80.608 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (472, 508472, 411, 490) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (479, 1770, 5055) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14207) [M::mem_pestat] mean and std.dev: (2990.02, 2872.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18783) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 206, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657) [M::mem_pestat] mean and std.dev: (236.44, 123.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 829) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (398, 2036, 4849) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13751) [M::mem_pestat] mean and std.dev: (3016.47, 2863.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18202) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (455, 1541, 4708) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13214) [M::mem_pestat] mean and std.dev: (2825.94, 2887.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17467) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1297.746 CPU sec, 81.469 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (491, 507968, 413, 544) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (400, 2190, 5031) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14293) [M::mem_pestat] mean and std.dev: (3055.03, 2962.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18924) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 207, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660) [M::mem_pestat] mean and std.dev: (237.25, 124.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 833) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (549, 2085, 4994) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13884) [M::mem_pestat] mean and std.dev: (3050.94, 2894.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18329) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (462, 1713, 4886) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13734) [M::mem_pestat] mean and std.dev: (2871.95, 2839.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18158) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1302.063 CPU sec, 81.820 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (469, 507690, 464, 490) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (450, 1618, 4660) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13080) [M::mem_pestat] mean and std.dev: (2830.05, 2842.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17290) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 206, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657) [M::mem_pestat] mean and std.dev: (236.35, 123.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 829) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (740, 2527, 5689) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15587) [M::mem_pestat] mean and std.dev: (3293.06, 2891.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20536) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (358, 1819, 5278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15118) [M::mem_pestat] mean and std.dev: (2985.12, 3056.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20038) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1286.121 CPU sec, 80.810 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (512, 506425, 397, 510) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (467, 1945, 4833) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13565) [M::mem_pestat] mean and std.dev: (2928.56, 2792.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17931) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 208, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670) [M::mem_pestat] mean and std.dev: (239.60, 126.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 846) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (560, 2070, 5330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14870) [M::mem_pestat] mean and std.dev: (3179.71, 2955.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19640) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (343, 1512, 4262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12100) [M::mem_pestat] mean and std.dev: (2601.41, 2747.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16019) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1303.101 CPU sec, 81.948 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (468, 509398, 432, 511) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (426, 1816, 4870) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13758) [M::mem_pestat] mean and std.dev: (2940.01, 2844.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18202) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 207, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658) [M::mem_pestat] mean and std.dev: (236.85, 124.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 830) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (570, 2376, 5266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14658) [M::mem_pestat] mean and std.dev: (3216.54, 2880.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19354) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (416, 1633, 4718) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13322) [M::mem_pestat] mean and std.dev: (2859.86, 2915.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17624) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1304.481 CPU sec, 82.185 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (495, 509724, 460, 424) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (470, 1993, 4818) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13514) [M::mem_pestat] mean and std.dev: (2959.72, 2836.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17862) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 206, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657) [M::mem_pestat] mean and std.dev: (236.11, 123.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 829) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (543, 2210, 4829) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13401) [M::mem_pestat] mean and std.dev: (2992.99, 2799.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17687) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (463, 1691, 4665) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13069) [M::mem_pestat] mean and std.dev: (2800.97, 2805.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17271) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1339.004 CPU sec, 84.015 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (494, 508563, 417, 488) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (401, 1709, 4989) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14165) [M::mem_pestat] mean and std.dev: (2905.60, 2936.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18753) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 205, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650) [M::mem_pestat] mean and std.dev: (234.13, 122.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 820) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (439, 1803, 4727) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13303) [M::mem_pestat] mean and std.dev: (2769.45, 2803.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17591) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (478, 1695, 4864) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13636) [M::mem_pestat] mean and std.dev: (2867.43, 2863.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18022) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1472.250 CPU sec, 92.360 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (462, 505225, 430, 479) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (458, 1843, 5162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14570) [M::mem_pestat] mean and std.dev: (2969.75, 2924.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19274) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 198, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 624) [M::mem_pestat] mean and std.dev: (227.07, 117.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 786) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (565, 2317, 5702) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15976) [M::mem_pestat] mean and std.dev: (3247.28, 3019.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21113) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (324, 1735, 5400) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15552) [M::mem_pestat] mean and std.dev: (3050.27, 3076.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20628) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1449.174 CPU sec, 91.171 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (462, 505816, 423, 478) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (475, 1761, 4820) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13510) [M::mem_pestat] mean and std.dev: (2828.40, 2883.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17855) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 205, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656) [M::mem_pestat] mean and std.dev: (235.14, 123.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 828) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (717, 2279, 4949) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13413) [M::mem_pestat] mean and std.dev: (3116.33, 2832.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17645) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (362, 1716, 4363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12365) [M::mem_pestat] mean and std.dev: (2819.08, 2876.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16366) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1353.845 CPU sec, 85.051 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (548, 506161, 463, 529) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (459, 1802, 5027) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14163) [M::mem_pestat] mean and std.dev: (2956.48, 2934.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18731) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 208, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670) [M::mem_pestat] mean and std.dev: (239.49, 126.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 846) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (442, 2227, 5076) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14344) [M::mem_pestat] mean and std.dev: (3063.36, 2881.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18978) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (400, 1829, 5146) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14638) [M::mem_pestat] mean and std.dev: (3006.63, 2932.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19384) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1302.938 CPU sec, 81.742 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (534, 508506, 435, 530) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (368, 1691, 5398) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15458) [M::mem_pestat] mean and std.dev: (3031.45, 3052.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20488) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 208, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661) [M::mem_pestat] mean and std.dev: (237.75, 124.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 834) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (784, 2259, 5185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13987) [M::mem_pestat] mean and std.dev: (3214.17, 2859.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18388) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (433, 1988, 5066) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14332) [M::mem_pestat] mean and std.dev: (3040.97, 2946.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18965) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1285.825 CPU sec, 80.689 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (526, 509510, 457, 582) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (465, 1943, 5075) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14295) [M::mem_pestat] mean and std.dev: (2890.43, 2736.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18905) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 209, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668) [M::mem_pestat] mean and std.dev: (239.76, 125.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 843) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (549, 2130, 5375) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15027) [M::mem_pestat] mean and std.dev: (3177.16, 2943.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19853) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (429, 1685, 4732) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13338) [M::mem_pestat] mean and std.dev: (2824.91, 2857.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17641) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1265.194 CPU sec, 79.535 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (495, 509333, 456, 526) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (373, 1625, 5356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15322) [M::mem_pestat] mean and std.dev: (2925.85, 2943.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20305) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 209, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668) [M::mem_pestat] mean and std.dev: (239.81, 126.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 843) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (716, 2430, 5777) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15899) [M::mem_pestat] mean and std.dev: (3455.84, 3106.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20960) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (346, 1710, 4764) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13600) [M::mem_pestat] mean and std.dev: (2813.60, 2893.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18018) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1249.850 CPU sec, 78.413 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (477, 510053, 451, 498) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (358, 1540, 4900) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13984) [M::mem_pestat] mean and std.dev: (2836.61, 2928.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18526) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 209, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665) [M::mem_pestat] mean and std.dev: (239.03, 125.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 839) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (605, 2247, 5826) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16268) [M::mem_pestat] mean and std.dev: (3268.55, 3031.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21489) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (334, 1474, 4230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12022) [M::mem_pestat] mean and std.dev: (2647.03, 2735.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15918) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1244.007 CPU sec, 78.109 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (538, 508980, 505, 499) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (503, 1983, 5250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14744) [M::mem_pestat] mean and std.dev: (3091.78, 2979.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19491) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 211, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675) [M::mem_pestat] mean and std.dev: (241.76, 127.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 852) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (560, 2413, 5552) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15536) [M::mem_pestat] mean and std.dev: (3266.26, 2909.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20528) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (361, 1600, 4361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12361) [M::mem_pestat] mean and std.dev: (2673.54, 2777.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16361) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1247.337 CPU sec, 78.311 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (507, 509908, 440, 549) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (456, 1764, 5468) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15492) [M::mem_pestat] mean and std.dev: (3063.79, 3001.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20504) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 211, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672) [M::mem_pestat] mean and std.dev: (241.46, 126.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 848) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (619, 2520, 5868) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16366) [M::mem_pestat] mean and std.dev: (3412.28, 3086.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21615) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (359, 1663, 5165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14777) [M::mem_pestat] mean and std.dev: (2992.53, 3079.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19583) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1243.726 CPU sec, 78.100 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (500, 510723, 391, 558) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (444, 1996, 5340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15132) [M::mem_pestat] mean and std.dev: (3069.49, 2899.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20028) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 210, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668) [M::mem_pestat] mean and std.dev: (240.30, 125.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 843) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (722, 2393, 5682) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15602) [M::mem_pestat] mean and std.dev: (3392.76, 3001.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20562) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (377, 1638, 4422) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12512) [M::mem_pestat] mean and std.dev: (2776.58, 2882.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16557) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1232.549 CPU sec, 77.396 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (478, 510442, 417, 509) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (410, 1282, 4662) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13166) [M::mem_pestat] mean and std.dev: (2765.55, 2899.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17418) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 208, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664) [M::mem_pestat] mean and std.dev: (238.30, 124.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 838) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (558, 2273, 5832) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16380) [M::mem_pestat] mean and std.dev: (3354.37, 3015.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21654) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (356, 1856, 5213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14927) [M::mem_pestat] mean and std.dev: (2975.92, 3001.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19784) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1251.675 CPU sec, 78.562 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (444, 510026, 431, 488) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (498, 2137, 5055) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14169) [M::mem_pestat] mean and std.dev: (3041.00, 2832.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18726) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 207, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655) [M::mem_pestat] mean and std.dev: (236.63, 123.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 826) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (486, 2177, 6014) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 17070) [M::mem_pestat] mean and std.dev: (3334.78, 3108.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 22598) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (362, 1573, 5410) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15506) [M::mem_pestat] mean and std.dev: (2992.93, 3088.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20554) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1291.099 CPU sec, 81.049 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (497, 510317, 417, 485) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (456, 1855, 5072) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14304) [M::mem_pestat] mean and std.dev: (2982.66, 2867.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18920) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 208, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655) [M::mem_pestat] mean and std.dev: (236.40, 122.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 826) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (569, 2452, 5202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14468) [M::mem_pestat] mean and std.dev: (3172.23, 2842.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19101) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (427, 1911, 4770) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13456) [M::mem_pestat] mean and std.dev: (2842.96, 2759.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17799) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1265.323 CPU sec, 79.460 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (564, 509364, 486, 522) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (447, 1927, 4677) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13137) [M::mem_pestat] mean and std.dev: (2868.57, 2813.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17367) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 209, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665) [M::mem_pestat] mean and std.dev: (239.30, 125.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 839) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (547, 2394, 5884) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16558) [M::mem_pestat] mean and std.dev: (3358.51, 3039.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21895) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (334, 1495, 4738) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13546) [M::mem_pestat] mean and std.dev: (2759.54, 2921.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17950) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1262.897 CPU sec, 79.494 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (483, 510009, 458, 568) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (395, 2132, 5093) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14489) [M::mem_pestat] mean and std.dev: (3012.73, 2888.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19187) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 208, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658) [M::mem_pestat] mean and std.dev: (236.86, 123.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 830) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (607, 2092, 5030) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13876) [M::mem_pestat] mean and std.dev: (3111.51, 2892.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18299) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (411, 1664, 4796) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13566) [M::mem_pestat] mean and std.dev: (2820.83, 2836.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17951) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1289.554 CPU sec, 80.972 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (466, 510488, 457, 479) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (454, 1997, 4916) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13840) [M::mem_pestat] mean and std.dev: (3011.03, 2893.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18302) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 208, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658) [M::mem_pestat] mean and std.dev: (236.93, 123.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 830) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (564, 1860, 4653) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12831) [M::mem_pestat] mean and std.dev: (2909.14, 2821.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16920) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (397, 1868, 5064) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14398) [M::mem_pestat] mean and std.dev: (2974.92, 2953.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19065) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1276.556 CPU sec, 80.224 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (452, 511230, 421, 508) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (355, 1697, 5148) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14734) [M::mem_pestat] mean and std.dev: (2866.29, 2910.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19527) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 206, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649) [M::mem_pestat] mean and std.dev: (235.02, 121.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 818) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (538, 2261, 4960) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13804) [M::mem_pestat] mean and std.dev: (3091.30, 2897.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18226) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (408, 1643, 4674) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13206) [M::mem_pestat] mean and std.dev: (2887.10, 2985.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17472) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1305.246 CPU sec, 81.972 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (435, 509317, 435, 474) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (428, 1594, 5246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14882) [M::mem_pestat] mean and std.dev: (2908.73, 2904.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19700) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 200, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 624) [M::mem_pestat] mean and std.dev: (227.35, 116.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 786) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (498, 2267, 5755) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16269) [M::mem_pestat] mean and std.dev: (3279.95, 3007.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21526) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (403, 1868, 4452) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12550) [M::mem_pestat] mean and std.dev: (2829.55, 2787.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16599) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1394.938 CPU sec, 87.538 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (479, 505381, 429, 466) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (389, 1479, 4714) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13364) [M::mem_pestat] mean and std.dev: (2762.95, 2861.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17689) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 203, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643) [M::mem_pestat] mean and std.dev: (231.84, 121.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 811) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (699, 2368, 5533) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15201) [M::mem_pestat] mean and std.dev: (3272.19, 2957.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20035) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (317, 1325, 4622) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13232) [M::mem_pestat] mean and std.dev: (2693.93, 2924.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17537) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1368.419 CPU sec, 85.858 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (677, 463105, 452, 678) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (367, 1603, 4829) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13753) [M::mem_pestat] mean and std.dev: (2827.60, 2886.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18215) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 201, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658) [M::mem_pestat] mean and std.dev: (232.36, 125.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 832) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (624, 2863, 5895) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16437) [M::mem_pestat] mean and std.dev: (3502.63, 3003.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21708) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (280, 1554, 4583) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13189) [M::mem_pestat] mean and std.dev: (2802.19, 2957.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17492) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1738.941 CPU sec, 109.034 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1209, 281810, 969, 1328) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (358, 1578, 4750) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13534) [M::mem_pestat] mean and std.dev: (2852.59, 2963.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17926) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (101, 148, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 467) [M::mem_pestat] mean and std.dev: (166.48, 89.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 589) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (613, 2320, 5524) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15346) [M::mem_pestat] mean and std.dev: (3248.80, 2928.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20257) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (322, 1578, 4536) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12964) [M::mem_pestat] mean and std.dev: (2727.07, 2855.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17178) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 3644.656 CPU sec, 272.017 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1342, 287501, 1097, 1446) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (340, 1593, 4890) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13990) [M::mem_pestat] mean and std.dev: (2850.20, 2940.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18540) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (103, 153, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (173.23, 95.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (602, 2224, 5478) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15230) [M::mem_pestat] mean and std.dev: (3208.67, 3006.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20106) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (321, 1528, 4546) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12996) [M::mem_pestat] mean and std.dev: (2742.73, 2887.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17221) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 3240.241 CPU sec, 203.672 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1381, 288730, 1098, 1404) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (345, 1400, 4516) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12858) [M::mem_pestat] mean and std.dev: (2704.94, 2853.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17029) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 154, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (175.23, 97.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 636) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (622, 2324, 5286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14614) [M::mem_pestat] mean and std.dev: (3163.03, 2869.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19278) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (363, 1431, 4503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12783) [M::mem_pestat] mean and std.dev: (2681.99, 2817.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16923) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 2645.405 CPU sec, 166.395 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1421, 289815, 1168, 1471) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (347, 1515, 5017) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14357) [M::mem_pestat] mean and std.dev: (2864.79, 3003.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19027) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 155, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (176.01, 97.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 640) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (698, 2607, 5370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14714) [M::mem_pestat] mean and std.dev: (3322.99, 2958.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19386) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (375, 1499, 4417) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12501) [M::mem_pestat] mean and std.dev: (2714.05, 2832.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16543) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 3078.541 CPU sec, 193.823 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1340, 289852, 1215, 1489) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (410, 1750, 4898) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13874) [M::mem_pestat] mean and std.dev: (2850.33, 2821.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18362) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 156, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 510) [M::mem_pestat] mean and std.dev: (177.21, 98.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 645) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (651, 2473, 5283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14547) [M::mem_pestat] mean and std.dev: (3230.62, 2878.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19179) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (346, 1481, 4419) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12565) [M::mem_pestat] mean and std.dev: (2666.23, 2768.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16638) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 3033.733 CPU sec, 190.030 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1433, 291844, 1154, 1461) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (389, 1592, 4400) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12422) [M::mem_pestat] mean and std.dev: (2716.11, 2767.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16433) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 156, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 504) [M::mem_pestat] mean and std.dev: (176.53, 97.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 637) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (704, 2322, 5401) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14795) [M::mem_pestat] mean and std.dev: (3277.35, 2923.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19492) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (345, 1585, 4661) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13293) [M::mem_pestat] mean and std.dev: (2777.15, 2870.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17609) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 2986.885 CPU sec, 187.170 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1451, 293227, 1155, 1527) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (383, 1689, 4694) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13316) [M::mem_pestat] mean and std.dev: (2829.21, 2869.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17627) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 155, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (176.33, 98.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 640) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (668, 2740, 5732) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15860) [M::mem_pestat] mean and std.dev: (3433.07, 3010.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20924) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (376, 1530, 4631) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13141) [M::mem_pestat] mean and std.dev: (2747.65, 2837.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17396) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 2941.318 CPU sec, 184.271 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1447, 292392, 1176, 1486) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (404, 1750, 4789) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13559) [M::mem_pestat] mean and std.dev: (2886.10, 2906.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17944) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 156, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 510) [M::mem_pestat] mean and std.dev: (177.72, 99.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 645) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (847, 2594, 5590) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15076) [M::mem_pestat] mean and std.dev: (3408.91, 2922.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19819) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (349, 1457, 4353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12361) [M::mem_pestat] mean and std.dev: (2680.45, 2854.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16365) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 2929.972 CPU sec, 183.484 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1499, 293028, 1201, 1485) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (391, 1686, 4533) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12817) [M::mem_pestat] mean and std.dev: (2834.08, 2887.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16959) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 156, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 510) [M::mem_pestat] mean and std.dev: (177.60, 99.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 645) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (712, 2526, 5670) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15586) [M::mem_pestat] mean and std.dev: (3374.36, 2928.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20544) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (421, 1858, 4915) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13903) [M::mem_pestat] mean and std.dev: (2954.21, 2915.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18397) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 2954.409 CPU sec, 185.058 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1512, 290979, 1203, 1577) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (422, 1699, 4753) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13415) [M::mem_pestat] mean and std.dev: (2841.36, 2847.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17746) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 157, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 516) [M::mem_pestat] mean and std.dev: (178.85, 100.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 653) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (758, 2708, 5584) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15236) [M::mem_pestat] mean and std.dev: (3410.66, 2908.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20062) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (329, 1308, 4374) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12464) [M::mem_pestat] mean and std.dev: (2606.82, 2802.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16509) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 2941.982 CPU sec, 184.261 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1439, 293731, 1185, 1545) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (402, 1630, 4948) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14040) [M::mem_pestat] mean and std.dev: (2855.41, 2886.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18586) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 156, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 507) [M::mem_pestat] mean and std.dev: (177.01, 98.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 641) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (621, 2514, 5669) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15765) [M::mem_pestat] mean and std.dev: (3330.38, 2974.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20813) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (370, 1651, 4846) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13798) [M::mem_pestat] mean and std.dev: (2801.72, 2822.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18274) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 2918.592 CPU sec, 182.822 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1521, 294238, 1182, 1507) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (369, 1629, 4684) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13314) [M::mem_pestat] mean and std.dev: (2783.02, 2853.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17629) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 156, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 513) [M::mem_pestat] mean and std.dev: (178.03, 99.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 649) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (737, 2397, 5231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14219) [M::mem_pestat] mean and std.dev: (3179.16, 2843.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18713) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (323, 1710, 4636) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13262) [M::mem_pestat] mean and std.dev: (2724.95, 2754.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17575) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 2877.399 CPU sec, 180.243 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1494, 293648, 1223, 1509) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (352, 1584, 4549) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12943) [M::mem_pestat] mean and std.dev: (2730.93, 2833.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17140) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 156, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 510) [M::mem_pestat] mean and std.dev: (177.40, 98.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 645) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (674, 2332, 5401) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14855) [M::mem_pestat] mean and std.dev: (3312.69, 2980.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19582) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (346, 1541, 4774) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13630) [M::mem_pestat] mean and std.dev: (2756.24, 2826.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18058) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 2886.184 CPU sec, 180.759 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1472, 294968, 1182, 1540) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (421, 1880, 4957) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14029) [M::mem_pestat] mean and std.dev: (2916.52, 2899.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18565) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 156, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 507) [M::mem_pestat] mean and std.dev: (176.87, 98.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 641) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (723, 2536, 5435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14859) [M::mem_pestat] mean and std.dev: (3305.71, 2890.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19571) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (347, 1523, 4678) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13340) [M::mem_pestat] mean and std.dev: (2751.46, 2868.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17671) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 2924.488 CPU sec, 183.167 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1449, 297156, 1149, 1475) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (427, 1646, 4820) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13606) [M::mem_pestat] mean and std.dev: (2851.98, 2876.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17999) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 154, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 497) [M::mem_pestat] mean and std.dev: (174.49, 95.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 628) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (608, 2308, 5450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15134) [M::mem_pestat] mean and std.dev: (3208.91, 2949.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19976) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (349, 1643, 4745) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13537) [M::mem_pestat] mean and std.dev: (2787.57, 2849.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17933) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 2926.775 CPU sec, 183.267 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1430, 298418, 1146, 1500) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (334, 1704, 4837) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13843) [M::mem_pestat] mean and std.dev: (2828.71, 2921.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18346) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 154, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 497) [M::mem_pestat] mean and std.dev: (174.22, 95.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 628) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (659, 2415, 5337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14693) [M::mem_pestat] mean and std.dev: (3247.70, 2913.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19371) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (337, 1545, 4225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12001) [M::mem_pestat] mean and std.dev: (2628.90, 2761.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15889) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 2937.258 CPU sec, 183.972 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1405, 298063, 1141, 1478) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (389, 1628, 4906) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13940) [M::mem_pestat] mean and std.dev: (2855.43, 2885.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18457) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 154, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 497) [M::mem_pestat] mean and std.dev: (174.46, 95.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 628) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (731, 2368, 5584) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15290) [M::mem_pestat] mean and std.dev: (3316.69, 2932.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20143) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (324, 1510, 4395) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12537) [M::mem_pestat] mean and std.dev: (2679.82, 2809.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16608) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 2926.154 CPU sec, 183.328 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1453, 297333, 1164, 1393) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (374, 1724, 4719) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13409) [M::mem_pestat] mean and std.dev: (2830.05, 2871.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17754) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 155, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 497) [M::mem_pestat] mean and std.dev: (174.74, 95.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 628) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (617, 2298, 5332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14762) [M::mem_pestat] mean and std.dev: (3190.72, 2925.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19477) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (367, 1476, 4499) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12763) [M::mem_pestat] mean and std.dev: (2651.90, 2794.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16895) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 2972.047 CPU sec, 186.153 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1347, 291116, 1197, 1505) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (379, 1685, 4714) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13384) [M::mem_pestat] mean and std.dev: (2858.88, 2892.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17719) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 155, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (177.49, 99.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 648) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (623, 2155, 5433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15053) [M::mem_pestat] mean and std.dev: (3122.88, 2918.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19863) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (343, 1501, 4629) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13201) [M::mem_pestat] mean and std.dev: (2655.46, 2776.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17487) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 2975.842 CPU sec, 186.498 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1455, 298463, 1149, 1517) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (371, 1656, 4858) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13832) [M::mem_pestat] mean and std.dev: (2883.91, 2940.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18319) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 155, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (175.25, 96.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 632) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (660, 2373, 5313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14619) [M::mem_pestat] mean and std.dev: (3244.54, 2921.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19272) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (337, 1535, 4583) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13075) [M::mem_pestat] mean and std.dev: (2766.45, 2902.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17321) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 2951.173 CPU sec, 184.869 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1449, 298160, 1074, 1485) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (413, 1596, 4542) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12800) [M::mem_pestat] mean and std.dev: (2786.50, 2835.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16929) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 155, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (175.52, 96.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 636) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (676, 2451, 5846) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16186) [M::mem_pestat] mean and std.dev: (3375.92, 3011.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21356) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (351, 1508, 4407) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12519) [M::mem_pestat] mean and std.dev: (2666.30, 2769.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16575) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 2965.944 CPU sec, 186.044 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1472, 298256, 1147, 1424) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (366, 1423, 4403) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12477) [M::mem_pestat] mean and std.dev: (2664.58, 2804.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16514) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 155, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (175.51, 96.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 636) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (580, 2253, 5348) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14884) [M::mem_pestat] mean and std.dev: (3135.87, 2857.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19652) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (399, 1761, 5182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14748) [M::mem_pestat] mean and std.dev: (2919.09, 2949.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19531) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 2991.931 CPU sec, 187.375 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1400, 300048, 1064, 1410) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (394, 1622, 4749) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13459) [M::mem_pestat] mean and std.dev: (2774.01, 2810.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17814) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 154, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 494) [M::mem_pestat] mean and std.dev: (173.64, 94.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 624) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (568, 2125, 5533) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15463) [M::mem_pestat] mean and std.dev: (3174.81, 2990.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20428) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (400, 1730, 4836) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13708) [M::mem_pestat] mean and std.dev: (2882.20, 2895.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18144) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 3015.352 CPU sec, 188.979 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1427, 299784, 1142, 1380) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (405, 1857, 4826) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13668) [M::mem_pestat] mean and std.dev: (2937.65, 2924.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18089) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 154, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 497) [M::mem_pestat] mean and std.dev: (173.99, 95.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 628) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (648, 2252, 5312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14640) [M::mem_pestat] mean and std.dev: (3140.04, 2865.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19304) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (419, 1810, 5039) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14279) [M::mem_pestat] mean and std.dev: (2985.14, 2947.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18899) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 3051.855 CPU sec, 191.134 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1439, 298265, 1153, 1515) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (407, 1685, 4876) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13814) [M::mem_pestat] mean and std.dev: (2866.33, 2898.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18283) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (103, 154, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (174.43, 96.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 631) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (588, 2148, 5051) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13977) [M::mem_pestat] mean and std.dev: (3131.32, 2944.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18440) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (343, 1409, 4572) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13030) [M::mem_pestat] mean and std.dev: (2685.34, 2867.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17259) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 3153.058 CPU sec, 202.490 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1443, 290625, 1205, 1387) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (407, 1865, 5038) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14300) [M::mem_pestat] mean and std.dev: (2982.96, 2883.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18931) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 156, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 513) [M::mem_pestat] mean and std.dev: (178.01, 99.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 649) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (564, 2311, 5352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14928) [M::mem_pestat] mean and std.dev: (3172.63, 2902.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19716) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (378, 1680, 4662) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13230) [M::mem_pestat] mean and std.dev: (2826.31, 2886.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17514) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 2977.339 CPU sec, 186.462 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1436, 290500, 1130, 1513) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (442, 1858, 4647) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13057) [M::mem_pestat] mean and std.dev: (2870.99, 2842.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17262) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 155, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (177.13, 99.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 648) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (747, 2624, 5669) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15513) [M::mem_pestat] mean and std.dev: (3441.01, 3003.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20435) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (329, 1564, 4794) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13724) [M::mem_pestat] mean and std.dev: (2761.45, 2882.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18189) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 3027.982 CPU sec, 189.773 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1464, 292498, 1217, 1497) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (360, 1582, 4852) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13836) [M::mem_pestat] mean and std.dev: (2837.46, 2936.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18328) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 156, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 507) [M::mem_pestat] mean and std.dev: (176.88, 98.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 641) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (552, 2327, 5114) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14238) [M::mem_pestat] mean and std.dev: (3162.12, 2893.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18800) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (360, 1637, 4689) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13347) [M::mem_pestat] mean and std.dev: (2796.42, 2893.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17676) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 3007.303 CPU sec, 188.368 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1400, 291956, 1150, 1540) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (355, 1679, 4887) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13951) [M::mem_pestat] mean and std.dev: (2843.42, 2898.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18483) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 156, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 516) [M::mem_pestat] mean and std.dev: (178.62, 100.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 653) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (679, 2354, 5557) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15313) [M::mem_pestat] mean and std.dev: (3301.23, 2933.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20191) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (356, 1637, 4929) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14075) [M::mem_pestat] mean and std.dev: (2854.72, 2928.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18648) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 2915.749 CPU sec, 182.647 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1513, 291529, 1192, 1438) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (397, 1600, 5017) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14257) [M::mem_pestat] mean and std.dev: (2866.16, 2882.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18877) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 156, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 513) [M::mem_pestat] mean and std.dev: (178.13, 99.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 649) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (742, 2616, 5686) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15574) [M::mem_pestat] mean and std.dev: (3390.31, 2940.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20518) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (326, 1605, 4584) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13100) [M::mem_pestat] mean and std.dev: (2736.53, 2855.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17358) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 2980.345 CPU sec, 186.730 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1424, 292139, 1250, 1495) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (399, 1861, 5317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15153) [M::mem_pestat] mean and std.dev: (3016.82, 2944.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20071) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 156, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 513) [M::mem_pestat] mean and std.dev: (178.34, 100.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 649) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (688, 2468, 5539) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15241) [M::mem_pestat] mean and std.dev: (3327.99, 2921.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20092) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (346, 1500, 4722) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13474) [M::mem_pestat] mean and std.dev: (2774.22, 2884.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17850) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 2905.285 CPU sec, 182.055 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1262, 283537, 1113, 1468) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (375, 1392, 4442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12576) [M::mem_pestat] mean and std.dev: (2662.46, 2820.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16643) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (103, 152, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (172.63, 95.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 623) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (540, 2186, 5457) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15291) [M::mem_pestat] mean and std.dev: (3174.61, 3023.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20208) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (373, 1495, 4553) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12913) [M::mem_pestat] mean and std.dev: (2759.56, 2893.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17093) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 3421.740 CPU sec, 214.848 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1400, 291712, 1258, 1417) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (410, 1717, 4636) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13088) [M::mem_pestat] mean and std.dev: (2797.18, 2837.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17314) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 154, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (175.38, 97.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 636) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (607, 2218, 5292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14662) [M::mem_pestat] mean and std.dev: (3154.27, 2914.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19347) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (317, 1528, 4386) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12524) [M::mem_pestat] mean and std.dev: (2703.60, 2806.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16593) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 3087.365 CPU sec, 193.329 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1479, 295434, 1083, 1491) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (422, 1798, 4848) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13700) [M::mem_pestat] mean and std.dev: (2876.52, 2860.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18126) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 155, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (175.48, 97.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 636) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (660, 2403, 5493) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15159) [M::mem_pestat] mean and std.dev: (3292.91, 2937.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19992) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (374, 1597, 4881) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13895) [M::mem_pestat] mean and std.dev: (2798.87, 2875.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18402) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 2977.162 CPU sec, 186.474 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1506, 296169, 1211, 1459) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (361, 1589, 4939) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14095) [M::mem_pestat] mean and std.dev: (2847.22, 2945.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18673) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 154, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (175.39, 97.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 636) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (531, 2226, 5248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14682) [M::mem_pestat] mean and std.dev: (3164.48, 2958.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19399) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (355, 1628, 4738) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13504) [M::mem_pestat] mean and std.dev: (2822.11, 2897.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17887) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 2978.732 CPU sec, 186.554 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1425, 295402, 1204, 1490) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (427, 1743, 4798) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13540) [M::mem_pestat] mean and std.dev: (2903.04, 2899.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17911) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 155, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (175.82, 97.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 640) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (670, 2282, 5457) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15031) [M::mem_pestat] mean and std.dev: (3246.70, 2962.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19818) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (311, 1380, 4644) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13310) [M::mem_pestat] mean and std.dev: (2663.71, 2806.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17643) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 2948.915 CPU sec, 184.736 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1394, 295488, 1164, 1384) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (390, 1736, 5070) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14430) [M::mem_pestat] mean and std.dev: (2915.08, 2909.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19110) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 155, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (175.60, 97.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 636) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (694, 2567, 5732) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15808) [M::mem_pestat] mean and std.dev: (3379.93, 2979.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20846) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (344, 1550, 4944) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14144) [M::mem_pestat] mean and std.dev: (2842.04, 2936.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18744) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 3037.984 CPU sec, 190.296 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1509, 296063, 1159, 1472) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (358, 1451, 4596) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13072) [M::mem_pestat] mean and std.dev: (2730.70, 2880.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17310) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 154, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (174.53, 96.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 632) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (569, 2276, 5208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14486) [M::mem_pestat] mean and std.dev: (3140.63, 2858.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19125) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (368, 1692, 4969) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14171) [M::mem_pestat] mean and std.dev: (2900.16, 2929.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18772) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 2961.525 CPU sec, 185.531 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1467, 295928, 1123, 1458) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (341, 1561, 4500) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12818) [M::mem_pestat] mean and std.dev: (2687.88, 2753.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16977) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 154, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (174.70, 96.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 632) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (545, 2376, 5201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14513) [M::mem_pestat] mean and std.dev: (3183.67, 2929.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19169) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (350, 1607, 4778) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13634) [M::mem_pestat] mean and std.dev: (2819.30, 2885.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18062) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 3051.833 CPU sec, 191.426 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1468, 294923, 1124, 1479) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (425, 1700, 4960) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14030) [M::mem_pestat] mean and std.dev: (2896.04, 2910.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18565) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 155, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (175.85, 98.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 640) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (744, 2719, 5898) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16206) [M::mem_pestat] mean and std.dev: (3479.74, 2986.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21360) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (365, 1608, 4804) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13682) [M::mem_pestat] mean and std.dev: (2796.08, 2885.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18121) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 3022.750 CPU sec, 189.361 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1411, 290388, 1121, 1429) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (317, 1313, 4549) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13013) [M::mem_pestat] mean and std.dev: (2687.49, 2900.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17245) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 153, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 497) [M::mem_pestat] mean and std.dev: (174.08, 96.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 628) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (575, 2521, 5467) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15251) [M::mem_pestat] mean and std.dev: (3289.72, 2963.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20143) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (393, 1647, 4694) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13296) [M::mem_pestat] mean and std.dev: (2758.30, 2787.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17597) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 3234.781 CPU sec, 202.588 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1459, 290199, 1146, 1399) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (374, 1583, 4859) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13829) [M::mem_pestat] mean and std.dev: (2814.23, 2897.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18314) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (103, 154, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502) [M::mem_pestat] mean and std.dev: (174.71, 97.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 635) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (652, 2392, 5451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15049) [M::mem_pestat] mean and std.dev: (3256.90, 2929.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19848) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (383, 1772, 4969) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14141) [M::mem_pestat] mean and std.dev: (2880.53, 2868.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18727) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 3180.356 CPU sec, 199.182 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1521, 295855, 1126, 1392) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (341, 1447, 4959) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14195) [M::mem_pestat] mean and std.dev: (2784.79, 2929.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18813) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 155, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (176.16, 98.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 640) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (682, 2283, 5425) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14911) [M::mem_pestat] mean and std.dev: (3197.83, 2910.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19654) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (376, 1667, 4965) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14143) [M::mem_pestat] mean and std.dev: (2884.17, 2924.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18732) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 3110.170 CPU sec, 196.525 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1448, 291432, 1126, 1381) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (377, 1695, 4767) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13547) [M::mem_pestat] mean and std.dev: (2819.78, 2872.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17937) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (103, 153, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (173.29, 95.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (641, 2156, 5362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14804) [M::mem_pestat] mean and std.dev: (3246.08, 2996.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19525) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (387, 1628, 4517) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12777) [M::mem_pestat] mean and std.dev: (2732.81, 2782.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16907) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 3449.957 CPU sec, 228.179 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1230, 281893, 1008, 1234) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (343, 1427, 4667) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13315) [M::mem_pestat] mean and std.dev: (2763.35, 2915.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17639) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (100, 147, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 463) [M::mem_pestat] mean and std.dev: (165.29, 88.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 584) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (755, 2623, 5837) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16001) [M::mem_pestat] mean and std.dev: (3403.45, 2947.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21083) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (320, 1602, 4652) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13316) [M::mem_pestat] mean and std.dev: (2745.69, 2899.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17648) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 3642.941 CPU sec, 228.524 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1308, 282439, 1027, 1367) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (375, 1785, 4944) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14082) [M::mem_pestat] mean and std.dev: (2909.54, 2900.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18651) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (103, 152, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (172.64, 95.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 623) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (719, 2544, 5418) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14816) [M::mem_pestat] mean and std.dev: (3297.36, 2906.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19515) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (352, 1498, 4754) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13558) [M::mem_pestat] mean and std.dev: (2786.88, 2873.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17960) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 3469.240 CPU sec, 217.238 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1341, 283545, 1107, 1455) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (407, 1762, 4937) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13997) [M::mem_pestat] mean and std.dev: (2907.18, 2923.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18527) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (103, 153, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (174.23, 97.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 631) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (637, 2218, 4987) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13687) [M::mem_pestat] mean and std.dev: (3101.18, 2833.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18037) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (356, 1506, 4772) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13604) [M::mem_pestat] mean and std.dev: (2800.16, 2942.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18020) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 3305.810 CPU sec, 207.002 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1475, 284869, 1130, 1313) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (453, 1732, 4685) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13149) [M::mem_pestat] mean and std.dev: (2857.03, 2843.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17381) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 154, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (175.94, 98.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 640) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (578, 2246, 5402) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15050) [M::mem_pestat] mean and std.dev: (3183.97, 2929.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19874) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (328, 1557, 4941) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14167) [M::mem_pestat] mean and std.dev: (2829.39, 2951.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18780) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 3626.009 CPU sec, 227.081 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1408, 286071, 1080, 1380) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (405, 1778, 5147) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14631) [M::mem_pestat] mean and std.dev: (2952.79, 2938.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19373) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 155, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (176.77, 98.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 644) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (836, 2492, 5842) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15854) [M::mem_pestat] mean and std.dev: (3456.47, 3010.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20860) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (366, 1526, 4839) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13785) [M::mem_pestat] mean and std.dev: (2802.44, 2877.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18258) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 3382.072 CPU sec, 212.101 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1388, 286626, 1159, 1403) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (412, 1668, 4817) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13627) [M::mem_pestat] mean and std.dev: (2863.00, 2882.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18032) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 156, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 518) [M::mem_pestat] mean and std.dev: (179.05, 101.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 656) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (701, 2390, 5682) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15644) [M::mem_pestat] mean and std.dev: (3354.31, 2942.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20625) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (362, 1676, 4940) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14096) [M::mem_pestat] mean and std.dev: (2899.31, 2953.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18674) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 3239.282 CPU sec, 202.849 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1383, 284516, 1172, 1385) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (415, 1822, 4501) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12673) [M::mem_pestat] mean and std.dev: (2804.51, 2792.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16759) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 155, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (176.74, 99.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 644) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (765, 2550, 5561) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15153) [M::mem_pestat] mean and std.dev: (3409.23, 2965.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19949) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (358, 1734, 4917) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14035) [M::mem_pestat] mean and std.dev: (2898.76, 2920.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18594) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 3430.566 CPU sec, 214.792 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1409, 287431, 1183, 1475) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (357, 1601, 4689) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13353) [M::mem_pestat] mean and std.dev: (2833.86, 2935.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17685) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 157, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 519) [M::mem_pestat] mean and std.dev: (179.50, 101.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 657) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (629, 2173, 5495) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15227) [M::mem_pestat] mean and std.dev: (3189.86, 2944.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20093) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (379, 1694, 5007) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14263) [M::mem_pestat] mean and std.dev: (2900.60, 2924.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18891) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 3096.377 CPU sec, 193.899 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1485, 289221, 1128, 1488) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (385, 1753, 4763) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13519) [M::mem_pestat] mean and std.dev: (2872.09, 2907.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17897) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 156, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (177.84, 100.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 648) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (694, 2552, 5987) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16573) [M::mem_pestat] mean and std.dev: (3449.30, 3009.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21866) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (346, 1548, 4654) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13270) [M::mem_pestat] mean and std.dev: (2753.90, 2851.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17578) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 3087.965 CPU sec, 193.430 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1458, 290334, 1160, 1464) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (381, 1619, 4823) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13707) [M::mem_pestat] mean and std.dev: (2872.82, 2940.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18149) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 156, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 513) [M::mem_pestat] mean and std.dev: (178.24, 100.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 649) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (635, 2344, 5340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14750) [M::mem_pestat] mean and std.dev: (3234.87, 2935.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19455) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (364, 1525, 4678) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13306) [M::mem_pestat] mean and std.dev: (2747.95, 2831.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17620) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 3074.223 CPU sec, 192.561 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1429, 291201, 1085, 1427) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (366, 1538, 4396) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12456) [M::mem_pestat] mean and std.dev: (2738.68, 2853.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16486) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 156, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 510) [M::mem_pestat] mean and std.dev: (177.56, 99.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 645) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (719, 2540, 5710) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15692) [M::mem_pestat] mean and std.dev: (3381.28, 2979.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20683) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (332, 1393, 4367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12437) [M::mem_pestat] mean and std.dev: (2663.68, 2820.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16472) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 3094.299 CPU sec, 193.781 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1440, 291141, 1214, 1515) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (462, 1878, 4795) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13461) [M::mem_pestat] mean and std.dev: (2931.86, 2882.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17794) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 156, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 513) [M::mem_pestat] mean and std.dev: (177.82, 99.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 649) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (600, 2498, 5544) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15432) [M::mem_pestat] mean and std.dev: (3339.11, 2991.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20376) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (334, 1508, 4266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12130) [M::mem_pestat] mean and std.dev: (2653.01, 2821.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16062) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 3058.979 CPU sec, 191.596 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1358, 292008, 1175, 1405) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (385, 1544, 4407) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12451) [M::mem_pestat] mean and std.dev: (2726.77, 2868.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16473) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 155, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 510) [M::mem_pestat] mean and std.dev: (177.40, 99.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 645) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (686, 2393, 5396) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14816) [M::mem_pestat] mean and std.dev: (3281.62, 2967.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19526) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (359, 1650, 4623) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13151) [M::mem_pestat] mean and std.dev: (2747.80, 2823.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17415) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 3090.686 CPU sec, 193.800 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1396, 291614, 1224, 1462) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (415, 1598, 4590) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12940) [M::mem_pestat] mean and std.dev: (2795.13, 2876.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17115) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 156, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 510) [M::mem_pestat] mean and std.dev: (177.78, 99.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 645) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (684, 2306, 5271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14445) [M::mem_pestat] mean and std.dev: (3198.77, 2911.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19032) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (341, 1513, 5021) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14381) [M::mem_pestat] mean and std.dev: (2842.63, 2978.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19061) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 3080.505 CPU sec, 192.963 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1359, 292193, 1234, 1489) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (393, 1845, 4528) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12798) [M::mem_pestat] mean and std.dev: (2843.58, 2869.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16933) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 156, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 510) [M::mem_pestat] mean and std.dev: (177.43, 99.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 645) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (643, 2389, 5549) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15361) [M::mem_pestat] mean and std.dev: (3285.00, 2956.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20267) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (334, 1545, 4460) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12712) [M::mem_pestat] mean and std.dev: (2730.61, 2863.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16838) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 3066.280 CPU sec, 192.144 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1392, 284987, 1143, 1441) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (379, 1572, 4643) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13171) [M::mem_pestat] mean and std.dev: (2824.66, 2908.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17435) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 155, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (176.79, 99.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 644) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (827, 2641, 6015) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16391) [M::mem_pestat] mean and std.dev: (3516.48, 3059.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21579) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (353, 1612, 4609) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13121) [M::mem_pestat] mean and std.dev: (2784.23, 2887.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17377) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 3292.855 CPU sec, 206.185 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1554, 293874, 1224, 1476) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (405, 1683, 4993) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14169) [M::mem_pestat] mean and std.dev: (2883.62, 2897.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18757) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 157, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 516) [M::mem_pestat] mean and std.dev: (178.84, 100.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 653) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (659, 2437, 5480) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15122) [M::mem_pestat] mean and std.dev: (3275.15, 2923.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19943) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (381, 1617, 4840) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13758) [M::mem_pestat] mean and std.dev: (2858.22, 2930.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18217) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 3018.261 CPU sec, 189.143 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1454, 292510, 1258, 1540) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (395, 1409, 4296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12098) [M::mem_pestat] mean and std.dev: (2683.37, 2824.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15999) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 156, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 507) [M::mem_pestat] mean and std.dev: (176.94, 98.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 641) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (856, 2360, 5607) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15109) [M::mem_pestat] mean and std.dev: (3360.41, 2957.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19860) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (418, 1599, 4953) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14023) [M::mem_pestat] mean and std.dev: (2886.83, 2960.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18558) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 3058.072 CPU sec, 191.520 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1432, 295717, 1103, 1435) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (340, 1470, 4440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12640) [M::mem_pestat] mean and std.dev: (2690.07, 2867.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16740) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 153, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 497) [M::mem_pestat] mean and std.dev: (174.12, 96.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 628) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (722, 2480, 5686) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15614) [M::mem_pestat] mean and std.dev: (3373.63, 2950.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20578) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (372, 1572, 4454) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12618) [M::mem_pestat] mean and std.dev: (2751.44, 2867.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16700) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 3040.686 CPU sec, 190.455 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1476, 296387, 1159, 1499) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (396, 1587, 4542) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12834) [M::mem_pestat] mean and std.dev: (2772.57, 2836.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16980) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 154, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 497) [M::mem_pestat] mean and std.dev: (174.28, 96.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 628) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (723, 2768, 5626) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15432) [M::mem_pestat] mean and std.dev: (3374.94, 2884.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20335) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (399, 1636, 4678) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13236) [M::mem_pestat] mean and std.dev: (2789.55, 2798.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17515) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 3078.818 CPU sec, 192.840 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1423, 295259, 1197, 1447) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (330, 1544, 4788) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13704) [M::mem_pestat] mean and std.dev: (2814.28, 2916.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18162) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 154, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 497) [M::mem_pestat] mean and std.dev: (174.38, 96.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 628) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (752, 2641, 6000) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16496) [M::mem_pestat] mean and std.dev: (3508.64, 3041.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21744) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (343, 1456, 4682) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13360) [M::mem_pestat] mean and std.dev: (2689.55, 2835.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17699) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 3055.610 CPU sec, 191.480 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1424, 295913, 1118, 1470) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (382, 1722, 4903) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13945) [M::mem_pestat] mean and std.dev: (2878.11, 2934.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18466) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 154, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (174.90, 96.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 632) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (635, 2137, 5144) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14162) [M::mem_pestat] mean and std.dev: (3150.60, 2915.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18671) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (315, 1432, 4646) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13308) [M::mem_pestat] mean and std.dev: (2726.87, 2856.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17639) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 3066.221 CPU sec, 192.017 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1477, 296152, 1105, 1402) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (390, 1590, 4499) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12717) [M::mem_pestat] mean and std.dev: (2759.02, 2820.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16826) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 154, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (174.81, 96.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 632) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (593, 2348, 5486) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15272) [M::mem_pestat] mean and std.dev: (3277.76, 3007.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20165) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (326, 1601, 4482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12794) [M::mem_pestat] mean and std.dev: (2702.98, 2817.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16950) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 3049.262 CPU sec, 191.001 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1496, 294894, 1134, 1464) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (399, 1859, 4904) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13914) [M::mem_pestat] mean and std.dev: (2951.98, 2907.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18419) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 154, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (174.92, 96.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 632) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (679, 2477, 5191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14215) [M::mem_pestat] mean and std.dev: (3206.61, 2830.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18727) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (337, 1589, 4792) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13702) [M::mem_pestat] mean and std.dev: (2796.01, 2899.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18157) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 3083.186 CPU sec, 193.432 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1423, 285886, 1112, 1440) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (420, 1799, 5073) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14379) [M::mem_pestat] mean and std.dev: (2947.95, 2901.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19032) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 155, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (177.09, 99.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 644) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (781, 2537, 5784) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15790) [M::mem_pestat] mean and std.dev: (3422.73, 2991.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20793) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (368, 1749, 4769) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13571) [M::mem_pestat] mean and std.dev: (2903.40, 2931.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17972) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 3237.439 CPU sec, 202.741 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1468, 295403, 1191, 1501) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (329, 1749, 5071) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14555) [M::mem_pestat] mean and std.dev: (2947.84, 3009.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19297) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 155, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (176.12, 98.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 640) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (757, 2602, 5673) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15505) [M::mem_pestat] mean and std.dev: (3391.07, 2957.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20421) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (375, 1605, 4631) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13143) [M::mem_pestat] mean and std.dev: (2829.90, 2898.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17399) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 3093.325 CPU sec, 193.769 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1495, 297421, 1167, 1407) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (354, 1708, 4685) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13347) [M::mem_pestat] mean and std.dev: (2807.58, 2853.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17678) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 154, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 497) [M::mem_pestat] mean and std.dev: (173.99, 96.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 628) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (620, 2246, 5610) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15590) [M::mem_pestat] mean and std.dev: (3283.14, 3005.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20580) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (353, 1588, 4398) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12488) [M::mem_pestat] mean and std.dev: (2698.13, 2814.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16533) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 3129.537 CPU sec, 196.015 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1493, 296929, 1157, 1462) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (334, 1460, 4260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12112) [M::mem_pestat] mean and std.dev: (2690.25, 2866.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16038) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 154, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (175.20, 97.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 636) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (707, 2713, 5889) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16253) [M::mem_pestat] mean and std.dev: (3412.86, 2936.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21435) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (338, 1474, 4724) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13496) [M::mem_pestat] mean and std.dev: (2778.60, 2915.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17882) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 3157.617 CPU sec, 197.768 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1454, 296241, 1161, 1480) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (443, 1780, 4944) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13946) [M::mem_pestat] mean and std.dev: (2884.88, 2873.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18447) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 155, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (175.30, 97.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 636) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (671, 2583, 5515) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15203) [M::mem_pestat] mean and std.dev: (3336.87, 2957.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20047) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (413, 1833, 4754) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13436) [M::mem_pestat] mean and std.dev: (2902.91, 2864.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17777) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 3377.647 CPU sec, 213.050 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1372, 287660, 1046, 1339) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (362, 1473, 4502) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12782) [M::mem_pestat] mean and std.dev: (2692.20, 2854.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16922) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (101, 148, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470) [M::mem_pestat] mean and std.dev: (166.87, 89.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (597, 2430, 5287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14667) [M::mem_pestat] mean and std.dev: (3229.07, 2949.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19357) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (409, 1759, 4869) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13789) [M::mem_pestat] mean and std.dev: (2860.75, 2860.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18249) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 3777.011 CPU sec, 236.666 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1231, 282763, 1000, 1313) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (340, 1596, 4685) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13375) [M::mem_pestat] mean and std.dev: (2816.66, 2917.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17720) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (101, 148, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470) [M::mem_pestat] mean and std.dev: (166.88, 89.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (601, 2247, 5424) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15070) [M::mem_pestat] mean and std.dev: (3174.27, 2988.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19893) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (294, 1331, 4888) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14076) [M::mem_pestat] mean and std.dev: (2745.70, 2984.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18670) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 3724.366 CPU sec, 233.244 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1345, 283567, 1025, 1375) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (349, 1647, 4638) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13216) [M::mem_pestat] mean and std.dev: (2754.40, 2837.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17505) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (102, 151, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 483) [M::mem_pestat] mean and std.dev: (169.64, 92.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 610) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (481, 2040, 5367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15139) [M::mem_pestat] mean and std.dev: (3136.36, 2997.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20025) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (341, 1501, 4429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12605) [M::mem_pestat] mean and std.dev: (2702.14, 2851.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16693) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 4333.122 CPU sec, 271.256 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1425, 284900, 1143, 1368) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (388, 1729, 5241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14947) [M::mem_pestat] mean and std.dev: (3016.89, 3053.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19800) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (103, 152, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (172.36, 95.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 623) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (631, 2421, 5607) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15559) [M::mem_pestat] mean and std.dev: (3294.81, 2943.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20535) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (350, 1480, 4878) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13934) [M::mem_pestat] mean and std.dev: (2766.91, 2873.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18462) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 4268.902 CPU sec, 267.265 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1435, 286141, 1132, 1456) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (360, 1596, 4630) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13170) [M::mem_pestat] mean and std.dev: (2769.37, 2878.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17440) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 155, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (176.73, 99.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 644) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (592, 2397, 5590) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15586) [M::mem_pestat] mean and std.dev: (3287.46, 2967.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20584) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (295, 1304, 4149) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11857) [M::mem_pestat] mean and std.dev: (2501.75, 2743.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15711) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 4055.043 CPU sec, 253.880 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1410, 287736, 1130, 1465) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (364, 1878, 4861) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13855) [M::mem_pestat] mean and std.dev: (2909.53, 2918.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18352) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 154, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (174.68, 96.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 632) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (635, 2463, 5360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14810) [M::mem_pestat] mean and std.dev: (3249.33, 2976.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19535) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (347, 1542, 4724) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13478) [M::mem_pestat] mean and std.dev: (2770.83, 2869.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17855) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 4059.187 CPU sec, 254.111 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1468, 289464, 1222, 1488) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (395, 1587, 4724) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13382) [M::mem_pestat] mean and std.dev: (2827.12, 2922.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17711) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 154, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (174.78, 96.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 632) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (649, 2492, 5842) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16228) [M::mem_pestat] mean and std.dev: (3433.40, 3079.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21421) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (355, 1573, 4631) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13183) [M::mem_pestat] mean and std.dev: (2796.41, 2892.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17459) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 4019.743 CPU sec, 251.637 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1476, 289929, 1119, 1357) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (343, 1489, 4774) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13636) [M::mem_pestat] mean and std.dev: (2838.09, 2972.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18067) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 155, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (176.03, 98.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 640) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (627, 2256, 5343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14775) [M::mem_pestat] mean and std.dev: (3194.92, 2922.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19491) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (386, 1758, 4854) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13790) [M::mem_pestat] mean and std.dev: (2846.32, 2838.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18258) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 3917.504 CPU sec, 245.252 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1461, 290424, 1193, 1423) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (383, 1573, 5067) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14435) [M::mem_pestat] mean and std.dev: (2837.40, 2892.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19119) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 155, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 504) [M::mem_pestat] mean and std.dev: (176.10, 97.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 637) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (702, 2194, 5412) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14832) [M::mem_pestat] mean and std.dev: (3199.81, 2912.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19542) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (314, 1425, 4273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12191) [M::mem_pestat] mean and std.dev: (2648.95, 2835.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16150) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 3810.788 CPU sec, 238.572 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1379, 292715, 1189, 1464) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (394, 1522, 4424) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12484) [M::mem_pestat] mean and std.dev: (2661.61, 2751.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16514) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 155, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (175.29, 97.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 636) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (698, 2548, 5691) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15677) [M::mem_pestat] mean and std.dev: (3368.87, 2978.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20670) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (321, 1490, 4924) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14130) [M::mem_pestat] mean and std.dev: (2814.24, 2940.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18733) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 3810.063 CPU sec, 238.529 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1413, 293116, 1187, 1396) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (369, 1723, 4707) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13383) [M::mem_pestat] mean and std.dev: (2825.99, 2847.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17721) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 155, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 507) [M::mem_pestat] mean and std.dev: (176.76, 98.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 641) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (604, 2593, 5662) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15778) [M::mem_pestat] mean and std.dev: (3365.32, 3011.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20836) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (373, 1738, 4992) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14230) [M::mem_pestat] mean and std.dev: (2900.52, 2947.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18849) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 3735.053 CPU sec, 233.839 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1515, 293557, 1136, 1505) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (414, 1644, 4717) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13323) [M::mem_pestat] mean and std.dev: (2798.17, 2870.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17626) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 155, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 504) [M::mem_pestat] mean and std.dev: (175.89, 97.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 637) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (556, 2205, 5551) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15541) [M::mem_pestat] mean and std.dev: (3183.09, 2949.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20536) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (363, 1524, 4809) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13701) [M::mem_pestat] mean and std.dev: (2821.03, 2932.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18147) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 3758.682 CPU sec, 235.338 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1446, 295150, 1175, 1437) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (357, 1457, 4743) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13515) [M::mem_pestat] mean and std.dev: (2737.62, 2868.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17901) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 155, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 501) [M::mem_pestat] mean and std.dev: (175.54, 97.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 633) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (614, 2125, 5482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15218) [M::mem_pestat] mean and std.dev: (3204.81, 2964.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20086) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (329, 1370, 4192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11918) [M::mem_pestat] mean and std.dev: (2575.33, 2765.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15781) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 3735.409 CPU sec, 233.877 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1557, 288848, 1149, 1430) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (395, 1685, 4951) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14063) [M::mem_pestat] mean and std.dev: (2845.43, 2881.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18619) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 156, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 513) [M::mem_pestat] mean and std.dev: (178.07, 100.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 649) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (633, 2244, 5236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14442) [M::mem_pestat] mean and std.dev: (3159.97, 2918.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19045) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (347, 1627, 4703) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13415) [M::mem_pestat] mean and std.dev: (2794.71, 2879.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17771) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 3759.505 CPU sec, 235.372 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1441, 292587, 1085, 1468) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (406, 1872, 4896) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13876) [M::mem_pestat] mean and std.dev: (2907.69, 2881.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18366) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 156, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 510) [M::mem_pestat] mean and std.dev: (177.83, 99.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 645) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (725, 2477, 5436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14858) [M::mem_pestat] mean and std.dev: (3301.62, 2946.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19569) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (375, 1668, 4989) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14217) [M::mem_pestat] mean and std.dev: (2889.64, 2938.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18831) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 3726.357 CPU sec, 233.304 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1491, 294381, 1203, 1464) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (386, 1549, 4547) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12869) [M::mem_pestat] mean and std.dev: (2743.97, 2826.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17030) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 156, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 507) [M::mem_pestat] mean and std.dev: (176.89, 98.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 641) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (696, 2463, 5068) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13812) [M::mem_pestat] mean and std.dev: (3196.58, 2826.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18184) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (382, 1522, 4946) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14074) [M::mem_pestat] mean and std.dev: (2850.01, 2970.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18638) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 3716.042 CPU sec, 232.659 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1451, 293539, 1274, 1452) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (391, 1466, 4174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11740) [M::mem_pestat] mean and std.dev: (2662.72, 2833.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15523) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 156, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 507) [M::mem_pestat] mean and std.dev: (176.98, 98.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 641) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (735, 2564, 5775) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15855) [M::mem_pestat] mean and std.dev: (3389.83, 2999.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20895) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (361, 1537, 4698) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13372) [M::mem_pestat] mean and std.dev: (2764.92, 2836.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17709) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 3941.901 CPU sec, 246.788 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1441, 294784, 1177, 1518) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (357, 1317, 4495) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12771) [M::mem_pestat] mean and std.dev: (2645.20, 2850.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16909) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 156, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 504) [M::mem_pestat] mean and std.dev: (176.27, 97.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 637) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (621, 2518, 5553) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15417) [M::mem_pestat] mean and std.dev: (3298.61, 2942.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20349) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (318, 1438, 4918) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14118) [M::mem_pestat] mean and std.dev: (2787.80, 2960.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18718) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 3940.032 CPU sec, 246.663 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1483, 296532, 1201, 1469) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (397, 1844, 4584) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12958) [M::mem_pestat] mean and std.dev: (2823.45, 2772.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17145) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 155, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (175.58, 97.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 636) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (599, 2149, 5710) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15932) [M::mem_pestat] mean and std.dev: (3225.92, 3014.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21043) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (345, 1467, 4303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12219) [M::mem_pestat] mean and std.dev: (2652.56, 2827.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16177) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 3990.080 CPU sec, 249.827 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1399, 296956, 1182, 1533) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (376, 1733, 4732) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13444) [M::mem_pestat] mean and std.dev: (2822.33, 2826.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17800) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 154, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (175.20, 97.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 636) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (708, 2543, 5340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14604) [M::mem_pestat] mean and std.dev: (3294.32, 2906.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19236) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (354, 1593, 4847) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13833) [M::mem_pestat] mean and std.dev: (2777.29, 2864.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18326) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 3992.007 CPU sec, 249.946 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1399, 297205, 1228, 1470) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (373, 1595, 4600) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13054) [M::mem_pestat] mean and std.dev: (2758.65, 2853.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17281) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 155, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (174.96, 96.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 632) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (617, 2473, 5623) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15635) [M::mem_pestat] mean and std.dev: (3315.75, 2961.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20641) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (331, 1573, 4645) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13273) [M::mem_pestat] mean and std.dev: (2773.60, 2874.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17587) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 3981.060 CPU sec, 249.250 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1465, 297073, 1180, 1539) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (375, 1443, 4387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12411) [M::mem_pestat] mean and std.dev: (2676.62, 2826.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16423) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 154, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (174.90, 96.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 632) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (589, 2196, 5314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14764) [M::mem_pestat] mean and std.dev: (3171.95, 2933.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19489) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (318, 1420, 4597) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13155) [M::mem_pestat] mean and std.dev: (2678.71, 2845.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17434) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 3965.136 CPU sec, 248.236 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1412, 293222, 1164, 1528) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (384, 1685, 4378) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12366) [M::mem_pestat] mean and std.dev: (2818.94, 2906.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16360) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 156, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 507) [M::mem_pestat] mean and std.dev: (176.93, 98.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 641) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (652, 2524, 5764) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15988) [M::mem_pestat] mean and std.dev: (3388.43, 2967.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21100) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (381, 1685, 5003) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14247) [M::mem_pestat] mean and std.dev: (2878.62, 2896.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18869) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 3964.758 CPU sec, 248.213 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1464, 293377, 1109, 1501) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (370, 1567, 4667) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13261) [M::mem_pestat] mean and std.dev: (2799.78, 2858.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17558) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 155, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (176.58, 98.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 644) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (739, 2436, 5266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14320) [M::mem_pestat] mean and std.dev: (3265.01, 2881.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18847) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (419, 1796, 4691) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13235) [M::mem_pestat] mean and std.dev: (2892.91, 2913.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17507) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 3983.984 CPU sec, 249.419 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1427, 297303, 1180, 1418) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (368, 1630, 4521) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12827) [M::mem_pestat] mean and std.dev: (2780.58, 2849.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16980) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 155, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (175.13, 96.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 636) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (705, 2497, 5371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14703) [M::mem_pestat] mean and std.dev: (3261.16, 2854.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19369) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (340, 1388, 4615) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13165) [M::mem_pestat] mean and std.dev: (2711.06, 2885.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17440) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 4044.305 CPU sec, 253.202 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1447, 297119, 1156, 1456) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (339, 1599, 4843) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13851) [M::mem_pestat] mean and std.dev: (2826.36, 2936.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18355) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 155, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (176.00, 97.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 640) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (665, 2539, 5517) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15221) [M::mem_pestat] mean and std.dev: (3350.84, 2982.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20073) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (377, 1622, 4703) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13355) [M::mem_pestat] mean and std.dev: (2780.61, 2832.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17681) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 3931.090 CPU sec, 246.154 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1488, 299307, 1140, 1439) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (397, 1617, 4933) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14005) [M::mem_pestat] mean and std.dev: (2823.68, 2873.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18541) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 154, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 497) [M::mem_pestat] mean and std.dev: (174.39, 95.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 628) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (659, 2388, 5388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14846) [M::mem_pestat] mean and std.dev: (3250.64, 2882.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19575) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (346, 1616, 4538) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12922) [M::mem_pestat] mean and std.dev: (2798.13, 2888.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17114) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 3932.681 CPU sec, 246.237 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1483, 298618, 1116, 1451) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (410, 1779, 4889) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13847) [M::mem_pestat] mean and std.dev: (2893.26, 2888.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18326) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 154, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 497) [M::mem_pestat] mean and std.dev: (173.95, 95.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 628) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (757, 2563, 5540) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15106) [M::mem_pestat] mean and std.dev: (3347.39, 2919.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19889) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (353, 1446, 4448) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12638) [M::mem_pestat] mean and std.dev: (2695.97, 2822.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16733) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 4155.592 CPU sec, 260.190 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1297, 290726, 1053, 1321) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (349, 1630, 4931) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14095) [M::mem_pestat] mean and std.dev: (2806.95, 2856.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18677) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (101, 149, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470) [M::mem_pestat] mean and std.dev: (167.34, 89.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (603, 2194, 5304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14706) [M::mem_pestat] mean and std.dev: (3191.45, 2916.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19407) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (312, 1367, 4462) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12762) [M::mem_pestat] mean and std.dev: (2684.94, 2896.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16912) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 4644.596 CPU sec, 290.781 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1243, 284516, 1015, 1265) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (320, 1459, 4660) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13340) [M::mem_pestat] mean and std.dev: (2691.13, 2804.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17680) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (101, 148, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 467) [M::mem_pestat] mean and std.dev: (166.21, 88.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 589) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (593, 2215, 5586) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15572) [M::mem_pestat] mean and std.dev: (3187.74, 2951.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20565) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (341, 1491, 4543) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12947) [M::mem_pestat] mean and std.dev: (2709.20, 2831.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17149) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 4854.930 CPU sec, 303.940 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1343, 283618, 1049, 1343) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (325, 1477, 4839) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13867) [M::mem_pestat] mean and std.dev: (2778.71, 2884.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18381) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (102, 151, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 483) [M::mem_pestat] mean and std.dev: (170.24, 92.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 610) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (689, 2257, 5521) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15185) [M::mem_pestat] mean and std.dev: (3233.62, 2923.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20017) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (340, 1564, 4762) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13606) [M::mem_pestat] mean and std.dev: (2814.38, 2954.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18028) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 4971.288 CPU sec, 311.190 real sec [M::process] read 771010 sequences (115651500 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1457, 285264, 1128, 1363) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (365, 1494, 4682) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13316) [M::mem_pestat] mean and std.dev: (2749.21, 2871.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17633) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (103, 153, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (174.01, 96.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 631) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (653, 2432, 5574) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15416) [M::mem_pestat] mean and std.dev: (3262.28, 2942.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20337) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (313, 1320, 4407) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12595) [M::mem_pestat] mean and std.dev: (2589.40, 2815.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16689) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 4488.637 CPU sec, 280.939 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (845, 166614, 688, 817) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (503, 1864, 5231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14687) [M::mem_pestat] mean and std.dev: (3018.20, 2922.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19415) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 157, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 519) [M::mem_pestat] mean and std.dev: (179.53, 101.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 657) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (653, 2391, 5419) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14951) [M::mem_pestat] mean and std.dev: (3218.94, 2893.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19717) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (372, 1623, 4903) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13965) [M::mem_pestat] mean and std.dev: (2863.11, 2928.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18496) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 771010 reads in 2504.831 CPU sec, 156.791 real sec [main] Version: 0.7.17-r1188 [main] CMD: bwa mem -SP5M -t 20 /oak/stanford/groups/akundaje/marinovg/ENCODE4/datasets-gDNA/L2786-bdellovibrio_bacteriovorus-ICPB_3264_gDNA_cloudy/SPAdes-3.15.4-2023-04-19-B_b_gDNA_check.pass-V2/bwa-indexes/scaffolds.fa /oak/stanford/groups/akundaje/marinovg/ENCODE4/DNAzoo/2023-09-26-Novogene-NovaSeq/juicer-L2871+L2872+L2875+L2876_LB/splits/L2871+L2872+L2875+L2876_R1.fastq.gz /oak/stanford/groups/akundaje/marinovg/ENCODE4/DNAzoo/2023-09-26-Novogene-NovaSeq/juicer-L2871+L2872+L2875+L2876_LB/splits/L2871+L2872+L2875+L2876_R2.fastq.gz [main] Real time: 35717.059 sec; CPU: 567303.785 sec Picked up _JAVA_OPTIONS: -Xmx16384m Iterated 1000 times, returning estimate Picked up _JAVA_OPTIONS: -Xmx16384m Picked up _JAVA_OPTIONS: -Xmx16384m Picked up _JAVA_OPTIONS: -Xmx16384m Could not read hic file: Index: 0, Size: 0