[M::bwa_idx_load_from_disk] read 0 ALT contigs [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (809, 423640, 727, 852) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (398, 2019, 5435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15509) [M::mem_pestat] mean and std.dev: (3163.86, 3024.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20546) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 189, 271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 543) [M::mem_pestat] mean and std.dev: (209.03, 102.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 679) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (882, 2882, 5789) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15603) [M::mem_pestat] mean and std.dev: (3517.52, 2878.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20510) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (414, 2106, 5169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14679) [M::mem_pestat] mean and std.dev: (3075.40, 2940.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19434) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1286.167 CPU sec, 129.344 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (881, 416195, 803, 930) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (584, 2162, 5391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15005) [M::mem_pestat] mean and std.dev: (3188.98, 2935.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19812) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 195, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570) [M::mem_pestat] mean and std.dev: (216.90, 108.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 714) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1084, 2799, 5726) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15010) [M::mem_pestat] mean and std.dev: (3542.69, 2885.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19652) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (477, 2063, 4676) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13074) [M::mem_pestat] mean and std.dev: (2901.81, 2774.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17273) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 999.985 CPU sec, 62.923 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (889, 416163, 748, 906) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (547, 2408, 5484) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15358) [M::mem_pestat] mean and std.dev: (3252.00, 2941.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20295) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 196, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 576) [M::mem_pestat] mean and std.dev: (218.49, 109.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 722) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1169, 3207, 6697) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 17753) [M::mem_pestat] mean and std.dev: (3934.64, 3087.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 23281) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (473, 2086, 5399) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15251) [M::mem_pestat] mean and std.dev: (3143.23, 2970.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20177) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1043.058 CPU sec, 65.823 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (918, 417453, 683, 931) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (454, 2215, 5942) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16918) [M::mem_pestat] mean and std.dev: (3310.82, 3082.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 22406) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 195, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 574) [M::mem_pestat] mean and std.dev: (217.96, 108.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 719) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (997, 3133, 6094) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16288) [M::mem_pestat] mean and std.dev: (3725.11, 2948.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21385) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (461, 2257, 5357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15149) [M::mem_pestat] mean and std.dev: (3142.45, 2991.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20045) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1018.913 CPU sec, 64.128 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (902, 415305, 818, 903) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (500, 2076, 5325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14975) [M::mem_pestat] mean and std.dev: (3136.91, 2965.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19800) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 196, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 577) [M::mem_pestat] mean and std.dev: (218.97, 109.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 723) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (760, 3048, 6200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 17080) [M::mem_pestat] mean and std.dev: (3643.96, 3073.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 22520) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (456, 1958, 5460) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15468) [M::mem_pestat] mean and std.dev: (3094.24, 3034.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20472) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1013.063 CPU sec, 63.806 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (898, 414705, 741, 902) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (561, 2308, 5001) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13881) [M::mem_pestat] mean and std.dev: (3128.30, 2874.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18321) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 196, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 577) [M::mem_pestat] mean and std.dev: (219.07, 109.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 723) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (899, 3119, 5976) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16130) [M::mem_pestat] mean and std.dev: (3668.81, 2930.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21207) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (404, 1989, 5375) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15317) [M::mem_pestat] mean and std.dev: (3083.85, 2996.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20288) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1017.869 CPU sec, 64.068 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (925, 416743, 738, 868) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (434, 2215, 5606) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15950) [M::mem_pestat] mean and std.dev: (3197.66, 3049.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21122) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 196, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 577) [M::mem_pestat] mean and std.dev: (219.12, 109.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 723) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1119, 3229, 6159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16239) [M::mem_pestat] mean and std.dev: (3752.24, 2938.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21279) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (421, 1984, 5709) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16285) [M::mem_pestat] mean and std.dev: (3173.07, 3052.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21573) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1001.454 CPU sec, 63.009 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (896, 418860, 657, 900) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (424, 2122, 5436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15460) [M::mem_pestat] mean and std.dev: (3090.51, 2969.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20472) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 195, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570) [M::mem_pestat] mean and std.dev: (217.03, 107.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 714) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (961, 2885, 6274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16900) [M::mem_pestat] mean and std.dev: (3683.67, 3013.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 22213) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (495, 1988, 5693) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16089) [M::mem_pestat] mean and std.dev: (3198.07, 3046.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21287) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1009.272 CPU sec, 63.527 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (929, 421729, 756, 923) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (559, 2432, 5291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14755) [M::mem_pestat] mean and std.dev: (3184.09, 2855.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19487) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 194, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 561) [M::mem_pestat] mean and std.dev: (214.96, 105.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 702) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (789, 2844, 6077) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16653) [M::mem_pestat] mean and std.dev: (3624.44, 3049.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21941) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (427, 2020, 5513) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15685) [M::mem_pestat] mean and std.dev: (3139.08, 3017.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20771) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1020.316 CPU sec, 64.256 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (908, 420507, 694, 863) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (580, 2264, 5700) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15940) [M::mem_pestat] mean and std.dev: (3307.72, 3043.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21060) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 194, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 564) [M::mem_pestat] mean and std.dev: (215.73, 106.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 706) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (921, 3112, 6003) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16167) [M::mem_pestat] mean and std.dev: (3710.61, 2980.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21249) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (503, 2110, 5734) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16196) [M::mem_pestat] mean and std.dev: (3224.52, 3050.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21427) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1026.944 CPU sec, 64.679 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (859, 418463, 727, 878) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (487, 2227, 5268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14830) [M::mem_pestat] mean and std.dev: (3142.36, 2927.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19611) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 195, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570) [M::mem_pestat] mean and std.dev: (217.22, 108.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 714) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (825, 3192, 6144) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16782) [M::mem_pestat] mean and std.dev: (3679.94, 3019.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 22101) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (576, 2248, 6050) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16998) [M::mem_pestat] mean and std.dev: (3330.43, 3068.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 22472) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1027.183 CPU sec, 64.680 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (863, 420974, 696, 905) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (465, 2188, 5086) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14328) [M::mem_pestat] mean and std.dev: (3124.01, 2967.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18949) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 195, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567) [M::mem_pestat] mean and std.dev: (216.45, 106.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 710) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (996, 3026, 6097) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16299) [M::mem_pestat] mean and std.dev: (3731.67, 3008.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21400) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (440, 2045, 5109) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14447) [M::mem_pestat] mean and std.dev: (3081.16, 2959.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19116) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1020.591 CPU sec, 64.292 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (845, 423282, 673, 866) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (515, 2259, 5767) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16271) [M::mem_pestat] mean and std.dev: (3331.07, 3084.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21523) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 194, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 561) [M::mem_pestat] mean and std.dev: (215.04, 105.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 702) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (770, 2593, 5934) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16262) [M::mem_pestat] mean and std.dev: (3491.29, 2992.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21426) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (468, 1870, 5080) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14304) [M::mem_pestat] mean and std.dev: (3044.56, 2952.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18916) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1038.742 CPU sec, 65.463 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (857, 421045, 763, 903) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (629, 2565, 6116) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 17090) [M::mem_pestat] mean and std.dev: (3490.06, 3065.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 22577) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 195, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567) [M::mem_pestat] mean and std.dev: (216.09, 107.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 710) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1034, 3299, 6372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 17048) [M::mem_pestat] mean and std.dev: (3852.82, 3025.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 22386) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (461, 2208, 5487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15539) [M::mem_pestat] mean and std.dev: (3212.49, 3061.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20565) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1058.915 CPU sec, 66.701 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (842, 413211, 782, 858) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (480, 2037, 5265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14835) [M::mem_pestat] mean and std.dev: (3085.55, 2990.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19620) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 196, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 579) [M::mem_pestat] mean and std.dev: (219.11, 110.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 726) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1022, 3425, 6401) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 17159) [M::mem_pestat] mean and std.dev: (3807.97, 3001.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 22538) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (432, 1798, 4868) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13740) [M::mem_pestat] mean and std.dev: (2891.21, 2892.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18176) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1040.116 CPU sec, 65.443 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (929, 414577, 800, 856) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (557, 2448, 5665) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15881) [M::mem_pestat] mean and std.dev: (3306.00, 2985.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20989) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 196, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 577) [M::mem_pestat] mean and std.dev: (219.22, 109.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 723) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (821, 2894, 5697) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15449) [M::mem_pestat] mean and std.dev: (3545.44, 2948.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20325) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (420, 2003, 5537) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15771) [M::mem_pestat] mean and std.dev: (3160.03, 3058.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20888) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1022.418 CPU sec, 64.336 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (886, 414300, 710, 907) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (394, 1808, 4736) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13420) [M::mem_pestat] mean and std.dev: (2893.67, 2884.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17762) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 196, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 577) [M::mem_pestat] mean and std.dev: (219.22, 110.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 723) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (676, 3096, 6380) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 17788) [M::mem_pestat] mean and std.dev: (3713.99, 3128.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 23492) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (428, 2162, 5317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15095) [M::mem_pestat] mean and std.dev: (3147.91, 2979.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19984) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1014.951 CPU sec, 63.891 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (862, 416086, 754, 865) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (546, 2258, 5491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15381) [M::mem_pestat] mean and std.dev: (3242.52, 3018.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20326) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 193, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 566) [M::mem_pestat] mean and std.dev: (215.36, 107.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 709) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (937, 3055, 6202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16732) [M::mem_pestat] mean and std.dev: (3728.60, 2998.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21997) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (512, 2196, 5384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15128) [M::mem_pestat] mean and std.dev: (3174.18, 2955.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20000) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1143.857 CPU sec, 72.040 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (845, 416713, 764, 850) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (515, 2046, 5134) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14372) [M::mem_pestat] mean and std.dev: (3126.09, 2984.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18991) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 194, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570) [M::mem_pestat] mean and std.dev: (216.57, 108.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 714) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (914, 2884, 5882) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15818) [M::mem_pestat] mean and std.dev: (3598.17, 3014.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20786) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (458, 2106, 5448) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15428) [M::mem_pestat] mean and std.dev: (3127.42, 2988.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20418) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1047.790 CPU sec, 65.926 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (895, 418119, 763, 851) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (509, 2146, 5514) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15524) [M::mem_pestat] mean and std.dev: (3207.37, 2975.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20529) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 194, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570) [M::mem_pestat] mean and std.dev: (216.85, 108.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 714) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (883, 2961, 6110) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16564) [M::mem_pestat] mean and std.dev: (3628.25, 2993.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21791) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (504, 2346, 5560) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15672) [M::mem_pestat] mean and std.dev: (3329.05, 3069.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20728) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1027.585 CPU sec, 64.653 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (863, 420057, 766, 898) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (461, 1971, 5290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14948) [M::mem_pestat] mean and std.dev: (3050.49, 2931.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19777) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 194, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567) [M::mem_pestat] mean and std.dev: (215.99, 107.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 710) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (913, 3051, 6197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16765) [M::mem_pestat] mean and std.dev: (3664.62, 2965.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 22049) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (516, 2329, 5688) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16032) [M::mem_pestat] mean and std.dev: (3232.14, 2978.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21204) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1040.806 CPU sec, 65.527 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (911, 418859, 738, 905) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (518, 2516, 5277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14795) [M::mem_pestat] mean and std.dev: (3181.57, 2880.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19554) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 195, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570) [M::mem_pestat] mean and std.dev: (216.68, 107.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 714) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (877, 3117, 6210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16876) [M::mem_pestat] mean and std.dev: (3696.61, 3038.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 22209) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (502, 1963, 5112) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14332) [M::mem_pestat] mean and std.dev: (3072.06, 2932.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18942) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1040.108 CPU sec, 65.447 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (899, 416774, 738, 895) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (582, 2143, 5356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14904) [M::mem_pestat] mean and std.dev: (3151.07, 2936.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19678) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 193, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 569) [M::mem_pestat] mean and std.dev: (215.85, 108.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 713) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1149, 2993, 6049) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15849) [M::mem_pestat] mean and std.dev: (3725.29, 2965.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20749) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (540, 2181, 5221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14583) [M::mem_pestat] mean and std.dev: (3129.80, 2912.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19264) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1124.039 CPU sec, 70.755 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (875, 417578, 731, 877) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (509, 2039, 5343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15011) [M::mem_pestat] mean and std.dev: (3087.58, 2880.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19845) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 195, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 573) [M::mem_pestat] mean and std.dev: (217.76, 108.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 718) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (950, 2923, 5649) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15047) [M::mem_pestat] mean and std.dev: (3533.37, 2894.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19746) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (416, 2166, 5185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14723) [M::mem_pestat] mean and std.dev: (3081.78, 2918.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19492) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1083.150 CPU sec, 68.487 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (767, 423089, 701, 801) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (635, 2342, 5534) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15332) [M::mem_pestat] mean and std.dev: (3300.74, 3019.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20231) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 188, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 542) [M::mem_pestat] mean and std.dev: (208.25, 102.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 678) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (882, 3120, 6036) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16344) [M::mem_pestat] mean and std.dev: (3693.49, 3002.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21498) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (463, 2115, 5359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15151) [M::mem_pestat] mean and std.dev: (3062.35, 2868.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20047) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1297.696 CPU sec, 81.745 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (826, 414269, 699, 850) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (481, 2235, 5673) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16057) [M::mem_pestat] mean and std.dev: (3264.55, 3040.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21249) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 193, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 572) [M::mem_pestat] mean and std.dev: (216.61, 109.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 717) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (734, 2528, 5232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14228) [M::mem_pestat] mean and std.dev: (3254.84, 2833.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18726) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (386, 2005, 4904) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13940) [M::mem_pestat] mean and std.dev: (3009.04, 2952.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18458) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1162.325 CPU sec, 73.030 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (858, 414062, 747, 915) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (501, 2356, 5553) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15657) [M::mem_pestat] mean and std.dev: (3231.95, 3009.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20709) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 195, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 582) [M::mem_pestat] mean and std.dev: (219.73, 111.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 730) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1063, 3290, 6226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16552) [M::mem_pestat] mean and std.dev: (3798.96, 2974.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21715) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (440, 2204, 5694) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16202) [M::mem_pestat] mean and std.dev: (3185.98, 3029.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21456) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1081.455 CPU sec, 68.038 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (856, 410713, 763, 917) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (480, 2151, 5065) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14235) [M::mem_pestat] mean and std.dev: (3091.76, 2868.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18820) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 196, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 579) [M::mem_pestat] mean and std.dev: (219.23, 110.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 726) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1001, 2971, 6261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16781) [M::mem_pestat] mean and std.dev: (3693.73, 3023.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 22041) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (362, 1725, 5140) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14696) [M::mem_pestat] mean and std.dev: (2921.94, 2899.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19474) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1107.857 CPU sec, 69.703 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (869, 413271, 754, 910) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (441, 2065, 5308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15042) [M::mem_pestat] mean and std.dev: (3126.80, 2970.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19909) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 196, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 580) [M::mem_pestat] mean and std.dev: (220.05, 111.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 727) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (794, 2960, 5821) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15875) [M::mem_pestat] mean and std.dev: (3511.96, 2946.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20902) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (469, 2065, 5121) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14425) [M::mem_pestat] mean and std.dev: (3089.44, 2927.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19077) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1050.240 CPU sec, 66.062 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (885, 414158, 738, 909) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (503, 2007, 5211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14627) [M::mem_pestat] mean and std.dev: (3118.37, 2949.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19335) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 196, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 577) [M::mem_pestat] mean and std.dev: (219.09, 110.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 723) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1025, 2874, 6138) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16364) [M::mem_pestat] mean and std.dev: (3667.29, 3009.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21477) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (490, 2244, 5828) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16504) [M::mem_pestat] mean and std.dev: (3328.60, 3072.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21842) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1043.300 CPU sec, 65.644 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (885, 414551, 786, 885) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (564, 2276, 5656) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15840) [M::mem_pestat] mean and std.dev: (3283.09, 2990.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20932) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 196, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 580) [M::mem_pestat] mean and std.dev: (219.74, 110.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 727) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1096, 3134, 6299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16705) [M::mem_pestat] mean and std.dev: (3800.90, 2987.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21908) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (445, 2266, 5547) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15751) [M::mem_pestat] mean and std.dev: (3197.65, 2973.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20853) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1038.723 CPU sec, 65.364 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (871, 414870, 714, 856) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (557, 2167, 5321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14849) [M::mem_pestat] mean and std.dev: (3168.18, 2983.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19613) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 196, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 577) [M::mem_pestat] mean and std.dev: (218.81, 109.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 723) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (988, 2826, 6277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16855) [M::mem_pestat] mean and std.dev: (3673.66, 3053.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 22144) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (472, 2300, 5356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15124) [M::mem_pestat] mean and std.dev: (3183.09, 2963.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20008) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1031.459 CPU sec, 64.877 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (825, 413898, 753, 927) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (576, 2180, 5198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14442) [M::mem_pestat] mean and std.dev: (3102.88, 2850.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19064) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 195, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 579) [M::mem_pestat] mean and std.dev: (218.89, 110.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 726) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (929, 2913, 5925) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15917) [M::mem_pestat] mean and std.dev: (3692.02, 3052.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20913) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (468, 2081, 5790) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16434) [M::mem_pestat] mean and std.dev: (3231.25, 3059.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21756) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1076.501 CPU sec, 67.736 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (848, 415761, 707, 860) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (470, 2046, 5084) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14312) [M::mem_pestat] mean and std.dev: (3051.37, 2911.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18926) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 195, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 576) [M::mem_pestat] mean and std.dev: (218.23, 109.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 722) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (793, 2872, 5888) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16078) [M::mem_pestat] mean and std.dev: (3577.91, 3042.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21173) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (429, 2198, 5233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14841) [M::mem_pestat] mean and std.dev: (3137.27, 2987.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19645) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1018.830 CPU sec, 64.148 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (871, 418825, 713, 887) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (406, 2281, 5416) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15436) [M::mem_pestat] mean and std.dev: (3179.03, 2983.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20446) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 194, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567) [M::mem_pestat] mean and std.dev: (216.27, 107.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 710) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (815, 2517, 5608) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15194) [M::mem_pestat] mean and std.dev: (3385.71, 2910.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19987) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (351, 1869, 4971) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14211) [M::mem_pestat] mean and std.dev: (2982.67, 2951.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18831) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1037.387 CPU sec, 65.293 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (811, 418610, 724, 824) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (575, 2100, 5086) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14108) [M::mem_pestat] mean and std.dev: (3172.12, 2978.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18619) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 194, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567) [M::mem_pestat] mean and std.dev: (216.37, 107.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 710) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (694, 2950, 5607) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15433) [M::mem_pestat] mean and std.dev: (3481.98, 2972.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20346) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (444, 1852, 4906) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13830) [M::mem_pestat] mean and std.dev: (2919.81, 2877.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18292) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1038.955 CPU sec, 65.365 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (891, 418326, 721, 861) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (478, 2120, 5400) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15244) [M::mem_pestat] mean and std.dev: (3123.59, 2972.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20166) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 194, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567) [M::mem_pestat] mean and std.dev: (215.94, 107.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 710) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (966, 3309, 6106) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16386) [M::mem_pestat] mean and std.dev: (3756.55, 2964.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21526) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (430, 1935, 5567) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15841) [M::mem_pestat] mean and std.dev: (3169.24, 3071.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20978) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1033.392 CPU sec, 65.061 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (852, 415155, 782, 897) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (648, 2526, 5344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14736) [M::mem_pestat] mean and std.dev: (3266.63, 2920.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19432) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 195, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 576) [M::mem_pestat] mean and std.dev: (218.32, 109.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 722) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (925, 2806, 5975) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16075) [M::mem_pestat] mean and std.dev: (3563.13, 2966.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21125) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (465, 2178, 5242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14796) [M::mem_pestat] mean and std.dev: (3065.87, 2848.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19573) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1069.298 CPU sec, 67.334 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (926, 419867, 720, 913) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (602, 2336, 5994) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16778) [M::mem_pestat] mean and std.dev: (3383.45, 3068.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 22170) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 194, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567) [M::mem_pestat] mean and std.dev: (215.80, 107.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 710) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (933, 2854, 6118) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16488) [M::mem_pestat] mean and std.dev: (3718.86, 3024.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21673) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (555, 2379, 5913) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16629) [M::mem_pestat] mean and std.dev: (3321.83, 3051.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21987) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1065.649 CPU sec, 67.061 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (881, 419073, 712, 876) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (580, 2202, 5473) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15259) [M::mem_pestat] mean and std.dev: (3218.28, 2942.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20152) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 194, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570) [M::mem_pestat] mean and std.dev: (216.54, 108.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 714) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1072, 3461, 6326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16834) [M::mem_pestat] mean and std.dev: (3865.40, 2993.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 22088) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (489, 2224, 5737) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16233) [M::mem_pestat] mean and std.dev: (3241.40, 3022.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21481) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1105.712 CPU sec, 69.576 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (787, 426457, 695, 865) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (551, 2185, 5254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14660) [M::mem_pestat] mean and std.dev: (3105.99, 2892.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19363) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 186, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 535) [M::mem_pestat] mean and std.dev: (205.96, 100.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 669) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (715, 2720, 5839) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16087) [M::mem_pestat] mean and std.dev: (3467.78, 2966.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21211) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (408, 2098, 5020) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14244) [M::mem_pestat] mean and std.dev: (3007.99, 2889.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18856) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1288.214 CPU sec, 80.922 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (849, 418956, 679, 856) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (516, 2177, 5428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15252) [M::mem_pestat] mean and std.dev: (3147.25, 2935.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20164) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 192, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 562) [M::mem_pestat] mean and std.dev: (213.48, 106.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 704) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (831, 2652, 5860) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15918) [M::mem_pestat] mean and std.dev: (3503.76, 2981.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20947) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (519, 2353, 5533) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15561) [M::mem_pestat] mean and std.dev: (3222.47, 2982.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20575) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1166.570 CPU sec, 73.374 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (877, 414741, 754, 936) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (523, 2430, 5707) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16075) [M::mem_pestat] mean and std.dev: (3317.91, 3004.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21259) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 195, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 573) [M::mem_pestat] mean and std.dev: (217.52, 109.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 718) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (976, 3238, 6729) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 18235) [M::mem_pestat] mean and std.dev: (3959.49, 3192.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 23988) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (400, 1996, 5315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15145) [M::mem_pestat] mean and std.dev: (3088.42, 3018.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20060) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1091.081 CPU sec, 68.631 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (819, 417574, 770, 882) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (523, 2214, 5631) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15847) [M::mem_pestat] mean and std.dev: (3240.10, 3046.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20955) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 194, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567) [M::mem_pestat] mean and std.dev: (215.94, 107.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 710) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (803, 2886, 5964) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16286) [M::mem_pestat] mean and std.dev: (3572.56, 2932.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21447) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (492, 1941, 5188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14580) [M::mem_pestat] mean and std.dev: (3040.52, 2930.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19276) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1070.660 CPU sec, 67.353 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (909, 417651, 713, 919) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (487, 2227, 5425) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15301) [M::mem_pestat] mean and std.dev: (3190.82, 2995.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20239) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 195, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 573) [M::mem_pestat] mean and std.dev: (217.55, 108.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 718) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (919, 2830, 6122) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16528) [M::mem_pestat] mean and std.dev: (3669.19, 2993.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21731) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (432, 2106, 5529) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15723) [M::mem_pestat] mean and std.dev: (3236.39, 3064.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20820) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1043.025 CPU sec, 65.627 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (874, 417536, 743, 863) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (424, 2295, 5390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15322) [M::mem_pestat] mean and std.dev: (3173.98, 2941.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20288) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 195, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570) [M::mem_pestat] mean and std.dev: (217.23, 108.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 714) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (897, 2656, 5887) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15867) [M::mem_pestat] mean and std.dev: (3523.10, 2944.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20857) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (432, 1953, 5440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15456) [M::mem_pestat] mean and std.dev: (3058.39, 2932.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20464) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1026.522 CPU sec, 64.586 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (886, 417878, 782, 912) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (459, 1878, 5230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14772) [M::mem_pestat] mean and std.dev: (3036.03, 2961.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19543) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 195, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570) [M::mem_pestat] mean and std.dev: (217.01, 107.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 714) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (840, 3144, 6309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 17247) [M::mem_pestat] mean and std.dev: (3744.13, 3038.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 22716) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (467, 1904, 5717) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16217) [M::mem_pestat] mean and std.dev: (3151.95, 3048.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21467) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1025.587 CPU sec, 64.568 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (857, 413954, 777, 899) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (464, 2263, 5620) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15932) [M::mem_pestat] mean and std.dev: (3264.25, 3065.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21088) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 197, 287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 583) [M::mem_pestat] mean and std.dev: (220.47, 111.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 731) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1002, 2824, 5731) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15189) [M::mem_pestat] mean and std.dev: (3516.23, 2902.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19918) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (532, 2539, 5717) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16087) [M::mem_pestat] mean and std.dev: (3412.73, 3028.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21272) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1027.788 CPU sec, 64.712 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (834, 417627, 680, 906) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (368, 2034, 5264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15056) [M::mem_pestat] mean and std.dev: (3033.32, 2960.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19952) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 195, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 571) [M::mem_pestat] mean and std.dev: (217.80, 108.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 715) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1018, 3128, 6251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16717) [M::mem_pestat] mean and std.dev: (3765.90, 2983.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21950) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (480, 2235, 5630) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15930) [M::mem_pestat] mean and std.dev: (3248.87, 3039.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21080) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1007.355 CPU sec, 63.412 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (880, 418827, 787, 907) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (430, 1917, 5176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14668) [M::mem_pestat] mean and std.dev: (2963.73, 2881.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19414) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 195, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570) [M::mem_pestat] mean and std.dev: (217.07, 107.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 714) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (893, 2993, 6008) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16238) [M::mem_pestat] mean and std.dev: (3647.82, 2989.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21353) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (442, 1933, 5263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14905) [M::mem_pestat] mean and std.dev: (3048.64, 2913.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19726) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1007.867 CPU sec, 63.446 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (889, 420211, 795, 873) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (516, 2314, 5706) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16086) [M::mem_pestat] mean and std.dev: (3334.91, 3065.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21276) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 194, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567) [M::mem_pestat] mean and std.dev: (216.16, 106.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 710) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (875, 2997, 6110) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16580) [M::mem_pestat] mean and std.dev: (3674.96, 3017.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21815) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (490, 2360, 5876) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16648) [M::mem_pestat] mean and std.dev: (3331.36, 3071.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 22034) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1022.411 CPU sec, 64.338 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (872, 421071, 750, 930) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (562, 2473, 5589) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15643) [M::mem_pestat] mean and std.dev: (3308.22, 3022.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20670) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 195, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567) [M::mem_pestat] mean and std.dev: (216.02, 106.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 710) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (964, 3125, 6151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16525) [M::mem_pestat] mean and std.dev: (3713.22, 2971.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21712) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (510, 2223, 5721) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16143) [M::mem_pestat] mean and std.dev: (3287.43, 3034.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21354) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1026.365 CPU sec, 64.633 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (880, 416108, 741, 940) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (545, 2232, 5335) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14915) [M::mem_pestat] mean and std.dev: (3204.51, 2957.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19705) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 196, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 577) [M::mem_pestat] mean and std.dev: (218.66, 109.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 723) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (853, 2955, 6173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16813) [M::mem_pestat] mean and std.dev: (3684.91, 3034.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 22133) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (458, 2096, 5543) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15713) [M::mem_pestat] mean and std.dev: (3197.94, 3004.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20798) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1034.983 CPU sec, 65.159 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (839, 420753, 761, 887) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (517, 2138, 5646) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15904) [M::mem_pestat] mean and std.dev: (3221.10, 3045.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21033) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 195, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567) [M::mem_pestat] mean and std.dev: (216.39, 106.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 710) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (937, 2925, 5835) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15631) [M::mem_pestat] mean and std.dev: (3569.12, 2927.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20529) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (421, 2187, 5282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15004) [M::mem_pestat] mean and std.dev: (3165.53, 2983.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19865) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1014.447 CPU sec, 63.891 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (861, 423302, 726, 873) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (526, 2322, 5329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14935) [M::mem_pestat] mean and std.dev: (3139.38, 2875.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19738) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 194, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 561) [M::mem_pestat] mean and std.dev: (214.90, 105.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 702) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (998, 3376, 6421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 17267) [M::mem_pestat] mean and std.dev: (3848.11, 3053.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 22690) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (516, 2174, 5418) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15222) [M::mem_pestat] mean and std.dev: (3194.23, 2989.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20124) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1033.352 CPU sec, 65.060 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (795, 427109, 712, 796) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (488, 2283, 5570) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15734) [M::mem_pestat] mean and std.dev: (3221.49, 3042.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20816) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 188, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 536) [M::mem_pestat] mean and std.dev: (206.98, 100.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 670) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (918, 3137, 6073) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16383) [M::mem_pestat] mean and std.dev: (3686.34, 2948.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21538) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (574, 2191, 5561) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15535) [M::mem_pestat] mean and std.dev: (3230.60, 2960.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20522) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1247.658 CPU sec, 78.438 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (794, 417994, 691, 862) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (506, 2489, 5625) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15863) [M::mem_pestat] mean and std.dev: (3318.37, 3032.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20982) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 191, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 556) [M::mem_pestat] mean and std.dev: (212.42, 105.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 696) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1056, 2985, 5931) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15681) [M::mem_pestat] mean and std.dev: (3687.10, 2971.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20556) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (426, 2390, 5554) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15810) [M::mem_pestat] mean and std.dev: (3208.41, 2998.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20938) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1154.158 CPU sec, 72.591 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (831, 402230, 631, 757) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (594, 2456, 5438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15126) [M::mem_pestat] mean and std.dev: (3279.22, 2897.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19970) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 192, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 559) [M::mem_pestat] mean and std.dev: (213.16, 105.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 700) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (799, 2684, 6016) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16450) [M::mem_pestat] mean and std.dev: (3489.67, 2939.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21667) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (453, 2163, 5217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14745) [M::mem_pestat] mean and std.dev: (3097.38, 2902.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19509) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 2056.850 CPU sec, 143.345 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (816, 394809, 656, 813) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (388, 1807, 4531) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12817) [M::mem_pestat] mean and std.dev: (2801.75, 2812.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16960) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 198, 289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 589) [M::mem_pestat] mean and std.dev: (221.54, 112.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 739) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (787, 2817, 5998) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16420) [M::mem_pestat] mean and std.dev: (3537.38, 2978.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21631) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (496, 2060, 5365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15103) [M::mem_pestat] mean and std.dev: (3141.98, 2951.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19972) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 2093.401 CPU sec, 131.718 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (813, 393987, 679, 839) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (531, 2293, 5623) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15807) [M::mem_pestat] mean and std.dev: (3260.97, 2960.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20899) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597) [M::mem_pestat] mean and std.dev: (224.64, 114.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 749) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (837, 2717, 6198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16920) [M::mem_pestat] mean and std.dev: (3584.64, 3083.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 22281) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (466, 1705, 5185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14623) [M::mem_pestat] mean and std.dev: (3016.35, 2934.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19342) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1761.061 CPU sec, 110.560 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (906, 393933, 690, 846) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (484, 2250, 5007) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14053) [M::mem_pestat] mean and std.dev: (3081.77, 2876.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18576) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 202, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 601) [M::mem_pestat] mean and std.dev: (226.25, 115.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 754) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (949, 3225, 6348) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 17146) [M::mem_pestat] mean and std.dev: (3759.98, 2983.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 22545) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (515, 1865, 5175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14495) [M::mem_pestat] mean and std.dev: (3033.56, 2874.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19155) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1924.919 CPU sec, 120.812 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (889, 395603, 725, 896) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (468, 2232, 5321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15027) [M::mem_pestat] mean and std.dev: (3145.45, 2952.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19880) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 202, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 600) [M::mem_pestat] mean and std.dev: (225.41, 114.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (918, 3073, 6106) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16482) [M::mem_pestat] mean and std.dev: (3610.72, 2971.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21670) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (522, 2405, 5456) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15324) [M::mem_pestat] mean and std.dev: (3187.79, 2927.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20258) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1915.475 CPU sec, 120.170 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (813, 396101, 674, 837) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (570, 2292, 5788) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16224) [M::mem_pestat] mean and std.dev: (3310.91, 3019.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21442) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 202, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 600) [M::mem_pestat] mean and std.dev: (225.55, 114.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1035, 2976, 6204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16542) [M::mem_pestat] mean and std.dev: (3702.67, 2941.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21711) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (566, 2307, 5522) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15434) [M::mem_pestat] mean and std.dev: (3221.27, 2949.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20390) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1874.359 CPU sec, 117.649 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (807, 395893, 690, 860) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (560, 2241, 5494) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15362) [M::mem_pestat] mean and std.dev: (3142.59, 2866.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20296) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 203, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 604) [M::mem_pestat] mean and std.dev: (227.04, 116.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 758) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (843, 2946, 6115) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16659) [M::mem_pestat] mean and std.dev: (3646.43, 3070.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21931) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (496, 2073, 5373) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15127) [M::mem_pestat] mean and std.dev: (3118.71, 2932.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20004) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1857.283 CPU sec, 116.634 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (803, 393271, 689, 849) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (558, 2442, 5440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15204) [M::mem_pestat] mean and std.dev: (3232.29, 2934.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20086) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 204, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616) [M::mem_pestat] mean and std.dev: (229.51, 118.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1064, 2969, 5721) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15035) [M::mem_pestat] mean and std.dev: (3681.62, 2907.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19692) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (418, 1632, 4942) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13990) [M::mem_pestat] mean and std.dev: (2907.13, 2923.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18514) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1861.353 CPU sec, 116.897 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (848, 396893, 710, 893) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (498, 1931, 5134) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14406) [M::mem_pestat] mean and std.dev: (3010.93, 2878.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19042) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 202, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 604) [M::mem_pestat] mean and std.dev: (226.67, 115.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 758) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1105, 3347, 6267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16591) [M::mem_pestat] mean and std.dev: (3833.47, 2992.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21753) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (473, 1804, 4980) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13994) [M::mem_pestat] mean and std.dev: (2940.40, 2846.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18501) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1823.450 CPU sec, 114.442 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (846, 396006, 650, 858) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (448, 2374, 5829) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16591) [M::mem_pestat] mean and std.dev: (3315.16, 3101.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21972) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 203, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 604) [M::mem_pestat] mean and std.dev: (227.13, 115.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 758) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (970, 3510, 6312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16996) [M::mem_pestat] mean and std.dev: (3849.92, 2986.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 22338) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (475, 2040, 5566) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15748) [M::mem_pestat] mean and std.dev: (3208.27, 3104.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20839) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1818.350 CPU sec, 114.127 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (821, 395997, 722, 883) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (531, 2343, 5571) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15651) [M::mem_pestat] mean and std.dev: (3270.62, 3020.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20691) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 203, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 601) [M::mem_pestat] mean and std.dev: (226.45, 114.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 754) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (952, 2904, 6505) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 17611) [M::mem_pestat] mean and std.dev: (3753.93, 3119.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 23164) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (454, 1917, 5241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14815) [M::mem_pestat] mean and std.dev: (3137.45, 3013.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19602) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1815.503 CPU sec, 113.978 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (823, 399764, 676, 851) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (538, 2136, 5029) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14011) [M::mem_pestat] mean and std.dev: (3080.62, 2876.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18502) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 201, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595) [M::mem_pestat] mean and std.dev: (224.40, 113.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 746) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1109, 3496, 6119) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16139) [M::mem_pestat] mean and std.dev: (3845.59, 2961.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21149) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (442, 2085, 5085) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14371) [M::mem_pestat] mean and std.dev: (3090.39, 2941.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19014) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1836.634 CPU sec, 115.245 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (819, 400682, 734, 833) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (580, 2271, 5858) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16414) [M::mem_pestat] mean and std.dev: (3308.12, 3043.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21692) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 200, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591) [M::mem_pestat] mean and std.dev: (223.16, 112.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 741) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (920, 2756, 5908) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15884) [M::mem_pestat] mean and std.dev: (3596.90, 3006.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20872) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (442, 1976, 5343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15145) [M::mem_pestat] mean and std.dev: (3121.47, 3048.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20046) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1822.153 CPU sec, 114.292 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (845, 401109, 708, 820) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (432, 1988, 4552) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12792) [M::mem_pestat] mean and std.dev: (2821.91, 2751.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16912) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591) [M::mem_pestat] mean and std.dev: (223.42, 112.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 741) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (841, 2665, 5367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14419) [M::mem_pestat] mean and std.dev: (3379.53, 2890.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18945) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (488, 2350, 5479) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15461) [M::mem_pestat] mean and std.dev: (3249.78, 2995.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20452) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1820.241 CPU sec, 114.209 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (859, 396657, 690, 820) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (598, 2519, 6105) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 17119) [M::mem_pestat] mean and std.dev: (3394.12, 3044.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 22626) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 202, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603) [M::mem_pestat] mean and std.dev: (226.24, 115.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 757) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (903, 3149, 6565) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 17889) [M::mem_pestat] mean and std.dev: (3801.95, 3066.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 23551) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (502, 1984, 5750) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16246) [M::mem_pestat] mean and std.dev: (3191.21, 3001.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21494) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1859.076 CPU sec, 116.650 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (864, 399173, 658, 892) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (427, 1981, 5213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14785) [M::mem_pestat] mean and std.dev: (3050.34, 2932.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19571) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 202, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595) [M::mem_pestat] mean and std.dev: (225.03, 113.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 746) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (844, 2917, 5813) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15751) [M::mem_pestat] mean and std.dev: (3583.42, 2976.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20720) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (459, 2125, 5115) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14427) [M::mem_pestat] mean and std.dev: (3046.67, 2881.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19083) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1826.652 CPU sec, 114.650 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (851, 400252, 689, 810) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (479, 2149, 5536) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15650) [M::mem_pestat] mean and std.dev: (3165.09, 3004.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20707) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 202, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 598) [M::mem_pestat] mean and std.dev: (225.05, 113.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 750) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (918, 2747, 5899) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15861) [M::mem_pestat] mean and std.dev: (3599.19, 3006.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20842) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (501, 2250, 5299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14895) [M::mem_pestat] mean and std.dev: (3161.07, 2894.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19693) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1849.489 CPU sec, 116.081 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (767, 402668, 716, 840) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (616, 2355, 5456) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15136) [M::mem_pestat] mean and std.dev: (3264.92, 2922.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19976) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591) [M::mem_pestat] mean and std.dev: (223.31, 112.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 741) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (896, 2834, 5762) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15494) [M::mem_pestat] mean and std.dev: (3562.34, 2985.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20360) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (527, 1980, 5038) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14060) [M::mem_pestat] mean and std.dev: (3022.81, 2869.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18571) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1887.600 CPU sec, 118.484 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (845, 403132, 678, 850) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (461, 2046, 5420) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15338) [M::mem_pestat] mean and std.dev: (3115.49, 3001.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20297) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 200, 290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588) [M::mem_pestat] mean and std.dev: (222.39, 111.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 737) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (895, 2992, 6180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16750) [M::mem_pestat] mean and std.dev: (3680.28, 3013.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 22035) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (536, 2014, 5059) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14105) [M::mem_pestat] mean and std.dev: (3088.03, 2935.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18628) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1876.949 CPU sec, 117.809 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (813, 401668, 717, 836) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (515, 1773, 5102) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14276) [M::mem_pestat] mean and std.dev: (2933.09, 2855.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18863) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 200, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591) [M::mem_pestat] mean and std.dev: (223.34, 112.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 741) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (743, 2905, 6006) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16532) [M::mem_pestat] mean and std.dev: (3580.80, 3043.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21795) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (436, 2003, 5327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15109) [M::mem_pestat] mean and std.dev: (3061.19, 2931.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20000) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1965.247 CPU sec, 124.643 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (842, 392920, 752, 894) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (492, 2356, 5356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15084) [M::mem_pestat] mean and std.dev: (3233.42, 2982.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19948) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 202, 298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 610) [M::mem_pestat] mean and std.dev: (228.04, 117.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 766) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (889, 2855, 6087) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16483) [M::mem_pestat] mean and std.dev: (3664.18, 3048.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21681) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (472, 2423, 5537) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15667) [M::mem_pestat] mean and std.dev: (3279.04, 3011.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20732) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1892.847 CPU sec, 118.803 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (881, 394460, 711, 863) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (499, 2258, 5193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14581) [M::mem_pestat] mean and std.dev: (3141.22, 2936.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19275) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 202, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 607) [M::mem_pestat] mean and std.dev: (227.26, 116.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 762) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (715, 2479, 5486) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15028) [M::mem_pestat] mean and std.dev: (3316.18, 2924.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19799) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (492, 2160, 5308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14940) [M::mem_pestat] mean and std.dev: (3178.38, 3031.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19756) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1900.130 CPU sec, 119.216 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (862, 394066, 736, 905) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (436, 1839, 5308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15052) [M::mem_pestat] mean and std.dev: (3080.72, 2978.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19924) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 204, 298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 610) [M::mem_pestat] mean and std.dev: (228.32, 117.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 766) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (909, 2925, 6329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 17169) [M::mem_pestat] mean and std.dev: (3726.82, 3105.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 22589) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (517, 2143, 5631) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15859) [M::mem_pestat] mean and std.dev: (3247.05, 3045.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20973) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1862.171 CPU sec, 116.854 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (834, 394190, 712, 859) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (619, 2241, 5707) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15883) [M::mem_pestat] mean and std.dev: (3356.35, 3033.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20971) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 203, 298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 610) [M::mem_pestat] mean and std.dev: (228.24, 117.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 766) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1002, 2834, 5997) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15987) [M::mem_pestat] mean and std.dev: (3568.14, 2917.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20982) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (498, 2136, 5301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14907) [M::mem_pestat] mean and std.dev: (3115.47, 2923.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19710) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1881.466 CPU sec, 118.113 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (869, 393770, 706, 858) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (558, 2193, 5690) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15954) [M::mem_pestat] mean and std.dev: (3300.23, 3046.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21086) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 203, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 607) [M::mem_pestat] mean and std.dev: (227.73, 116.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 762) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (982, 2940, 6136) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16444) [M::mem_pestat] mean and std.dev: (3674.39, 3004.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21598) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (433, 2113, 5230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14824) [M::mem_pestat] mean and std.dev: (3066.70, 2916.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19621) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1889.988 CPU sec, 118.631 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (798, 393388, 674, 831) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (500, 2031, 4941) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13823) [M::mem_pestat] mean and std.dev: (3075.65, 2937.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18264) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 199, 290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 590) [M::mem_pestat] mean and std.dev: (222.18, 112.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 740) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (945, 2844, 6095) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16395) [M::mem_pestat] mean and std.dev: (3656.41, 3022.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21545) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (446, 2019, 5449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15455) [M::mem_pestat] mean and std.dev: (3120.72, 3048.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20458) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 2142.110 CPU sec, 134.516 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (886, 397380, 689, 868) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (391, 1900, 5008) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14242) [M::mem_pestat] mean and std.dev: (2961.33, 2918.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18859) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 202, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603) [M::mem_pestat] mean and std.dev: (226.04, 115.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 757) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (765, 2792, 5775) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15795) [M::mem_pestat] mean and std.dev: (3490.80, 2976.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20805) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (506, 2162, 5410) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15218) [M::mem_pestat] mean and std.dev: (3212.04, 2986.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20122) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1858.769 CPU sec, 116.654 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (918, 396734, 708, 834) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (534, 2084, 5196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14520) [M::mem_pestat] mean and std.dev: (3122.91, 2956.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19182) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 202, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 601) [M::mem_pestat] mean and std.dev: (225.94, 115.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 754) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (824, 2971, 5747) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15593) [M::mem_pestat] mean and std.dev: (3532.73, 2889.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20516) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (461, 2089, 5022) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14144) [M::mem_pestat] mean and std.dev: (3044.76, 2911.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18705) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1875.230 CPU sec, 117.675 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (828, 398289, 675, 837) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (569, 2040, 5147) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14303) [M::mem_pestat] mean and std.dev: (3139.56, 2958.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18881) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 202, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 600) [M::mem_pestat] mean and std.dev: (225.58, 114.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (894, 2723, 5915) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15957) [M::mem_pestat] mean and std.dev: (3544.36, 2975.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20978) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (491, 1986, 5072) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14234) [M::mem_pestat] mean and std.dev: (3008.66, 2858.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18815) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1881.532 CPU sec, 118.072 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (831, 398900, 716, 837) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (527, 2319, 5622) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15812) [M::mem_pestat] mean and std.dev: (3250.95, 2956.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20907) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597) [M::mem_pestat] mean and std.dev: (224.73, 114.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 749) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (893, 2748, 5813) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15653) [M::mem_pestat] mean and std.dev: (3515.35, 2923.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20573) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (511, 1875, 5239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14695) [M::mem_pestat] mean and std.dev: (3047.48, 2901.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19423) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1875.546 CPU sec, 117.738 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (854, 399226, 714, 820) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (621, 2282, 5385) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14913) [M::mem_pestat] mean and std.dev: (3117.57, 2807.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19677) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597) [M::mem_pestat] mean and std.dev: (224.89, 114.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 749) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1137, 3389, 6179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16263) [M::mem_pestat] mean and std.dev: (3839.04, 2982.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21305) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (479, 1990, 5282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14888) [M::mem_pestat] mean and std.dev: (3010.33, 2892.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19691) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1880.177 CPU sec, 117.968 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (844, 398475, 661, 834) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (456, 1939, 5070) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14298) [M::mem_pestat] mean and std.dev: (3060.81, 2949.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18912) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 202, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603) [M::mem_pestat] mean and std.dev: (225.96, 115.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 757) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (986, 3165, 6484) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 17480) [M::mem_pestat] mean and std.dev: (3790.48, 3035.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 22978) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (538, 2239, 5516) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15472) [M::mem_pestat] mean and std.dev: (3213.65, 2934.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20450) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1899.756 CPU sec, 119.241 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (782, 392514, 676, 796) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (548, 2143, 5513) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15443) [M::mem_pestat] mean and std.dev: (3183.75, 2942.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20408) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 199, 292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 596) [M::mem_pestat] mean and std.dev: (223.65, 114.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 748) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (959, 2590, 5918) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15836) [M::mem_pestat] mean and std.dev: (3522.92, 2951.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20795) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (515, 2369, 5480) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15410) [M::mem_pestat] mean and std.dev: (3180.45, 2898.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20375) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 2120.396 CPU sec, 133.230 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (857, 397413, 710, 853) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (515, 2172, 5312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14906) [M::mem_pestat] mean and std.dev: (3104.38, 2862.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19703) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 203, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 601) [M::mem_pestat] mean and std.dev: (226.58, 115.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 754) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (876, 2953, 5706) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15366) [M::mem_pestat] mean and std.dev: (3576.10, 2912.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20196) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (511, 2058, 5373) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15097) [M::mem_pestat] mean and std.dev: (3178.66, 3026.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19959) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1892.430 CPU sec, 118.854 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (729, 397492, 693, 759) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (554, 2313, 5501) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15395) [M::mem_pestat] mean and std.dev: (3236.44, 3020.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20342) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 196, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 573) [M::mem_pestat] mean and std.dev: (217.56, 108.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 718) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (883, 3159, 6268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 17038) [M::mem_pestat] mean and std.dev: (3781.00, 3043.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 22423) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (453, 2085, 5408) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15318) [M::mem_pestat] mean and std.dev: (3103.30, 2925.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20273) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 2279.881 CPU sec, 151.970 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (741, 393567, 656, 773) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (526, 2211, 5523) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15517) [M::mem_pestat] mean and std.dev: (3193.61, 2938.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20514) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 196, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 578) [M::mem_pestat] mean and std.dev: (218.41, 110.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 725) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1039, 3101, 6191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16495) [M::mem_pestat] mean and std.dev: (3755.43, 2989.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21647) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (437, 1756, 5066) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14324) [M::mem_pestat] mean and std.dev: (2974.29, 2968.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18953) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 2305.580 CPU sec, 144.580 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (746, 391291, 668, 824) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (531, 1820, 5156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14406) [M::mem_pestat] mean and std.dev: (3054.42, 2953.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19031) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 199, 292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 598) [M::mem_pestat] mean and std.dev: (223.76, 114.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 751) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (963, 3002, 6128) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16458) [M::mem_pestat] mean and std.dev: (3661.21, 2927.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21623) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (515, 2375, 5156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14438) [M::mem_pestat] mean and std.dev: (3139.47, 2914.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19079) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 2152.688 CPU sec, 134.993 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (871, 391910, 679, 827) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (552, 2659, 5746) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16134) [M::mem_pestat] mean and std.dev: (3360.93, 2993.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21328) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603) [M::mem_pestat] mean and std.dev: (226.03, 116.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 757) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (987, 3044, 6168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16530) [M::mem_pestat] mean and std.dev: (3687.77, 2979.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21711) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (483, 1918, 5204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14646) [M::mem_pestat] mean and std.dev: (3069.10, 2992.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19367) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 2070.756 CPU sec, 129.879 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (810, 391241, 661, 806) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (481, 2136, 5639) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15955) [M::mem_pestat] mean and std.dev: (3232.48, 3038.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21113) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 203, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616) [M::mem_pestat] mean and std.dev: (229.53, 118.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (958, 3192, 6279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16921) [M::mem_pestat] mean and std.dev: (3779.51, 2985.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 22242) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (447, 1844, 5240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14826) [M::mem_pestat] mean and std.dev: (3134.21, 3025.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19619) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1952.960 CPU sec, 122.601 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (804, 389562, 726, 848) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (472, 1847, 5054) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14218) [M::mem_pestat] mean and std.dev: (2981.87, 2844.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18800) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 202, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609) [M::mem_pestat] mean and std.dev: (227.29, 117.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 765) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1040, 3059, 6247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16661) [M::mem_pestat] mean and std.dev: (3776.20, 3031.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21868) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (469, 2054, 4984) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14014) [M::mem_pestat] mean and std.dev: (3042.71, 2876.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18529) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 2079.158 CPU sec, 130.424 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (833, 390557, 704, 863) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (544, 2211, 5570) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15622) [M::mem_pestat] mean and std.dev: (3222.44, 2950.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20648) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 204, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619) [M::mem_pestat] mean and std.dev: (230.47, 119.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (929, 2918, 5879) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15779) [M::mem_pestat] mean and std.dev: (3612.37, 3014.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20729) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (470, 2170, 5107) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14381) [M::mem_pestat] mean and std.dev: (3115.76, 2910.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19018) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1911.527 CPU sec, 119.991 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (824, 392931, 678, 828) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (454, 1989, 5149) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14539) [M::mem_pestat] mean and std.dev: (3050.46, 2932.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19234) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 202, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609) [M::mem_pestat] mean and std.dev: (227.81, 117.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 765) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (759, 2755, 5315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14427) [M::mem_pestat] mean and std.dev: (3390.94, 2902.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18983) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (454, 2195, 5429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15379) [M::mem_pestat] mean and std.dev: (3110.81, 2961.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20354) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1925.824 CPU sec, 120.875 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (860, 393127, 710, 861) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (487, 1831, 5516) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15574) [M::mem_pestat] mean and std.dev: (3095.54, 2983.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20603) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 203, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 607) [M::mem_pestat] mean and std.dev: (227.61, 116.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 762) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (768, 2657, 5736) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15672) [M::mem_pestat] mean and std.dev: (3460.81, 2950.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20640) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (493, 2465, 5538) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15628) [M::mem_pestat] mean and std.dev: (3302.82, 2981.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20673) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1898.850 CPU sec, 119.203 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (852, 394268, 656, 861) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (591, 2453, 5293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14697) [M::mem_pestat] mean and std.dev: (3213.18, 2850.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19399) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 203, 298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 610) [M::mem_pestat] mean and std.dev: (228.19, 117.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 766) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (908, 3113, 6249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16931) [M::mem_pestat] mean and std.dev: (3723.55, 3008.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 22272) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (511, 2135, 4694) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13060) [M::mem_pestat] mean and std.dev: (2963.05, 2783.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17243) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1875.982 CPU sec, 117.754 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (874, 394353, 738, 884) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (532, 2291, 5245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14671) [M::mem_pestat] mean and std.dev: (3107.36, 2850.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19384) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 203, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 607) [M::mem_pestat] mean and std.dev: (227.92, 116.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 762) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (936, 3212, 6418) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 17382) [M::mem_pestat] mean and std.dev: (3819.38, 3071.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 22864) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (456, 2348, 5505) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15603) [M::mem_pestat] mean and std.dev: (3209.69, 2958.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20652) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1894.137 CPU sec, 118.862 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (827, 393893, 683, 834) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (546, 2346, 5623) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15777) [M::mem_pestat] mean and std.dev: (3284.17, 3042.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20854) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 203, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 607) [M::mem_pestat] mean and std.dev: (227.73, 116.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 762) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (952, 2929, 6145) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16531) [M::mem_pestat] mean and std.dev: (3720.25, 3004.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21724) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (544, 2224, 5430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15202) [M::mem_pestat] mean and std.dev: (3224.52, 2948.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20088) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1861.779 CPU sec, 116.957 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (843, 391525, 717, 797) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (555, 2248, 5744) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16122) [M::mem_pestat] mean and std.dev: (3332.69, 3048.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21311) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 202, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606) [M::mem_pestat] mean and std.dev: (226.64, 116.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1241, 3326, 5954) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15380) [M::mem_pestat] mean and std.dev: (3764.98, 2935.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20093) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (472, 2363, 5208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14680) [M::mem_pestat] mean and std.dev: (3095.56, 2904.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19416) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 2008.169 CPU sec, 125.987 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (799, 394726, 663, 833) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (526, 2493, 5669) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15955) [M::mem_pestat] mean and std.dev: (3349.52, 3031.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21098) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 203, 298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 610) [M::mem_pestat] mean and std.dev: (228.30, 117.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 766) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (926, 2975, 5993) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16127) [M::mem_pestat] mean and std.dev: (3600.64, 2994.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21194) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (484, 2435, 5391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15205) [M::mem_pestat] mean and std.dev: (3264.25, 3019.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20112) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1856.552 CPU sec, 116.510 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (832, 396240, 647, 866) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (500, 2016, 5363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15089) [M::mem_pestat] mean and std.dev: (3122.24, 2944.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19952) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 202, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 601) [M::mem_pestat] mean and std.dev: (226.35, 115.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 754) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (805, 2805, 6334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 17392) [M::mem_pestat] mean and std.dev: (3589.97, 3010.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 22921) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (470, 2396, 5159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14537) [M::mem_pestat] mean and std.dev: (3154.91, 2932.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19226) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1859.268 CPU sec, 116.726 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (785, 398691, 739, 887) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (586, 2384, 5424) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15100) [M::mem_pestat] mean and std.dev: (3300.86, 2958.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19938) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597) [M::mem_pestat] mean and std.dev: (224.80, 114.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 749) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (978, 2963, 5932) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15840) [M::mem_pestat] mean and std.dev: (3633.46, 2938.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20794) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (612, 2467, 5737) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15987) [M::mem_pestat] mean and std.dev: (3339.54, 2918.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21112) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1871.540 CPU sec, 117.570 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (838, 398265, 727, 864) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (553, 2513, 5793) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16273) [M::mem_pestat] mean and std.dev: (3351.19, 3006.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21513) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597) [M::mem_pestat] mean and std.dev: (224.93, 114.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 749) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (841, 2825, 6108) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16642) [M::mem_pestat] mean and std.dev: (3617.39, 3049.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21909) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (545, 2202, 5053) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14069) [M::mem_pestat] mean and std.dev: (3099.00, 2885.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18577) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1879.553 CPU sec, 117.997 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (795, 398158, 678, 813) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (468, 2179, 5125) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14439) [M::mem_pestat] mean and std.dev: (3117.09, 2908.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19096) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597) [M::mem_pestat] mean and std.dev: (224.78, 114.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 749) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (894, 2769, 5845) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15747) [M::mem_pestat] mean and std.dev: (3556.12, 2947.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20698) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (563, 2041, 5202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14480) [M::mem_pestat] mean and std.dev: (3107.66, 2907.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19119) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1862.735 CPU sec, 116.996 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (823, 398190, 632, 869) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (471, 2290, 5657) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16029) [M::mem_pestat] mean and std.dev: (3297.94, 3052.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21215) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597) [M::mem_pestat] mean and std.dev: (225.16, 114.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 749) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (836, 2851, 5583) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15077) [M::mem_pestat] mean and std.dev: (3472.12, 2916.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19824) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (493, 2187, 5317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14965) [M::mem_pestat] mean and std.dev: (3138.15, 2961.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19789) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1852.191 CPU sec, 116.247 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (810, 393058, 634, 844) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (583, 2299, 5089) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14101) [M::mem_pestat] mean and std.dev: (3142.57, 2874.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18607) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 202, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 604) [M::mem_pestat] mean and std.dev: (226.60, 116.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 758) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (752, 2536, 5831) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15989) [M::mem_pestat] mean and std.dev: (3452.04, 2983.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21068) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (477, 1971, 5324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15018) [M::mem_pestat] mean and std.dev: (3051.92, 2883.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19865) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1974.263 CPU sec, 123.871 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (845, 396968, 686, 881) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (484, 1942, 5190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14602) [M::mem_pestat] mean and std.dev: (3060.44, 2920.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19308) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 600) [M::mem_pestat] mean and std.dev: (225.50, 114.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1035, 3175, 6221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16593) [M::mem_pestat] mean and std.dev: (3813.46, 3019.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21779) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (530, 2190, 5320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14900) [M::mem_pestat] mean and std.dev: (3160.85, 2931.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19690) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1882.378 CPU sec, 118.232 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (800, 399336, 674, 877) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (497, 2023, 5436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15314) [M::mem_pestat] mean and std.dev: (3133.11, 2976.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20253) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594) [M::mem_pestat] mean and std.dev: (224.10, 113.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 745) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (959, 2942, 6253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16841) [M::mem_pestat] mean and std.dev: (3755.99, 3048.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 22135) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (517, 2461, 5866) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16564) [M::mem_pestat] mean and std.dev: (3363.39, 3066.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21913) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1913.951 CPU sec, 120.091 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (814, 399911, 775, 864) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (471, 2176, 5452) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15414) [M::mem_pestat] mean and std.dev: (3192.71, 2998.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20395) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 600) [M::mem_pestat] mean and std.dev: (225.33, 114.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (860, 2848, 6108) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16604) [M::mem_pestat] mean and std.dev: (3616.35, 3015.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21852) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (505, 2007, 5470) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15400) [M::mem_pestat] mean and std.dev: (3125.02, 2968.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20365) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1966.100 CPU sec, 123.597 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (787, 399975, 629, 753) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (398, 1688, 5272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15020) [M::mem_pestat] mean and std.dev: (2970.25, 3021.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19894) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 194, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 566) [M::mem_pestat] mean and std.dev: (215.05, 106.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 709) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (822, 2661, 5830) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15846) [M::mem_pestat] mean and std.dev: (3464.42, 2900.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20854) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (441, 1946, 5488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15582) [M::mem_pestat] mean and std.dev: (3151.79, 3062.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20629) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 2323.110 CPU sec, 147.041 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (798, 394944, 612, 802) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (427, 2215, 5002) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14152) [M::mem_pestat] mean and std.dev: (3046.85, 2871.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18727) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 194, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 569) [M::mem_pestat] mean and std.dev: (215.67, 108.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 713) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (707, 2501, 6075) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16811) [M::mem_pestat] mean and std.dev: (3487.91, 3018.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 22179) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (434, 2139, 5553) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15791) [M::mem_pestat] mean and std.dev: (3159.24, 3030.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20910) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 2362.650 CPU sec, 148.243 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (860, 393690, 638, 772) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (500, 2145, 5363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15089) [M::mem_pestat] mean and std.dev: (3199.49, 2995.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19952) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 197, 287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 585) [M::mem_pestat] mean and std.dev: (219.98, 111.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 734) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1030, 2765, 5950) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15790) [M::mem_pestat] mean and std.dev: (3584.32, 2913.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20710) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (481, 2064, 5254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14800) [M::mem_pestat] mean and std.dev: (3122.71, 2971.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19573) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 2174.673 CPU sec, 136.415 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (854, 391649, 696, 860) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (506, 2372, 5713) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16127) [M::mem_pestat] mean and std.dev: (3277.98, 2986.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21334) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603) [M::mem_pestat] mean and std.dev: (225.82, 116.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 757) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (866, 2995, 6205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16883) [M::mem_pestat] mean and std.dev: (3665.89, 3008.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 22222) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (492, 1832, 5361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15099) [M::mem_pestat] mean and std.dev: (3073.42, 2997.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19968) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 2030.162 CPU sec, 127.393 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (837, 394687, 675, 805) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (588, 2357, 5585) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15579) [M::mem_pestat] mean and std.dev: (3277.01, 2963.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20576) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 200, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 593) [M::mem_pestat] mean and std.dev: (223.13, 113.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 744) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (876, 2896, 5594) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15030) [M::mem_pestat] mean and std.dev: (3499.96, 2928.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19748) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (459, 2296, 5227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14763) [M::mem_pestat] mean and std.dev: (3158.92, 2961.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19531) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 2044.895 CPU sec, 128.390 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (823, 395439, 741, 878) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (450, 1944, 4907) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13821) [M::mem_pestat] mean and std.dev: (3018.65, 2927.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18278) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 200, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597) [M::mem_pestat] mean and std.dev: (224.22, 114.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 749) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (868, 2791, 5941) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16087) [M::mem_pestat] mean and std.dev: (3528.50, 3003.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21160) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (462, 1875, 5213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14715) [M::mem_pestat] mean and std.dev: (3038.56, 2969.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19466) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1984.371 CPU sec, 124.534 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (855, 395379, 707, 865) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (495, 2304, 5768) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16314) [M::mem_pestat] mean and std.dev: (3267.85, 3032.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21587) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 600) [M::mem_pestat] mean and std.dev: (225.36, 114.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (854, 2764, 5906) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16010) [M::mem_pestat] mean and std.dev: (3533.97, 2982.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21062) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (556, 2327, 5828) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16372) [M::mem_pestat] mean and std.dev: (3391.54, 3082.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21644) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1936.849 CPU sec, 121.544 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (776, 396162, 687, 851) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (491, 2047, 5159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14495) [M::mem_pestat] mean and std.dev: (3044.75, 2899.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19163) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597) [M::mem_pestat] mean and std.dev: (224.37, 114.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 749) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (872, 2708, 5711) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15389) [M::mem_pestat] mean and std.dev: (3563.19, 2968.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20228) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (474, 1897, 5163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14541) [M::mem_pestat] mean and std.dev: (3062.99, 2931.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19230) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1911.583 CPU sec, 120.004 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (887, 396124, 762, 838) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (572, 2187, 5544) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15488) [M::mem_pestat] mean and std.dev: (3196.45, 2968.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20460) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 202, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 601) [M::mem_pestat] mean and std.dev: (226.06, 115.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 754) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (811, 2794, 6099) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16675) [M::mem_pestat] mean and std.dev: (3575.86, 3033.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21963) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (464, 1837, 5381) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15215) [M::mem_pestat] mean and std.dev: (3082.84, 3011.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20132) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1890.279 CPU sec, 118.680 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (835, 397520, 693, 794) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (486, 2012, 5756) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16296) [M::mem_pestat] mean and std.dev: (3194.97, 3033.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21566) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597) [M::mem_pestat] mean and std.dev: (224.57, 113.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 749) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (924, 2796, 6058) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16326) [M::mem_pestat] mean and std.dev: (3556.46, 2969.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21460) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (448, 1887, 5496) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15592) [M::mem_pestat] mean and std.dev: (3100.01, 3009.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20640) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1895.964 CPU sec, 119.053 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (823, 393585, 667, 850) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (647, 2742, 5726) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15884) [M::mem_pestat] mean and std.dev: (3401.82, 2932.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20963) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 203, 298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 610) [M::mem_pestat] mean and std.dev: (228.35, 117.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 766) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (791, 2742, 5993) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16397) [M::mem_pestat] mean and std.dev: (3551.46, 3022.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21599) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (432, 2093, 4923) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13905) [M::mem_pestat] mean and std.dev: (2927.57, 2797.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18396) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1886.265 CPU sec, 118.357 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (867, 394788, 707, 824) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (665, 2093, 5115) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14015) [M::mem_pestat] mean and std.dev: (3058.44, 2818.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18465) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 203, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 607) [M::mem_pestat] mean and std.dev: (227.61, 116.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 762) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1000, 2939, 5921) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15763) [M::mem_pestat] mean and std.dev: (3615.65, 2990.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20684) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (481, 2486, 5200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14638) [M::mem_pestat] mean and std.dev: (3156.86, 2902.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19357) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1870.801 CPU sec, 117.361 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (822, 398224, 662, 837) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (460, 2007, 5264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14872) [M::mem_pestat] mean and std.dev: (3059.64, 2933.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19676) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 202, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 601) [M::mem_pestat] mean and std.dev: (226.03, 114.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 754) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1169, 3336, 6373) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16781) [M::mem_pestat] mean and std.dev: (3892.18, 2974.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21985) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (486, 1885, 5160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14508) [M::mem_pestat] mean and std.dev: (3049.05, 2957.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19182) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1840.302 CPU sec, 115.462 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (795, 397675, 702, 859) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (496, 1980, 4846) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13546) [M::mem_pestat] mean and std.dev: (3024.55, 2888.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17896) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 202, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 601) [M::mem_pestat] mean and std.dev: (226.04, 114.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 754) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (973, 2776, 6083) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16303) [M::mem_pestat] mean and std.dev: (3593.67, 2952.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21413) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (543, 2329, 5612) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15750) [M::mem_pestat] mean and std.dev: (3246.95, 2965.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20819) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1838.785 CPU sec, 115.342 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (797, 398853, 677, 842) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (455, 1990, 5261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14873) [M::mem_pestat] mean and std.dev: (3073.86, 2934.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19679) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 201, 292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 592) [M::mem_pestat] mean and std.dev: (224.38, 113.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 742) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (928, 3091, 6401) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 17347) [M::mem_pestat] mean and std.dev: (3741.09, 2995.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 22820) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (475, 2090, 5159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14527) [M::mem_pestat] mean and std.dev: (3043.10, 2884.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19211) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1860.573 CPU sec, 116.951 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (829, 399545, 629, 895) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (558, 2392, 5453) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15243) [M::mem_pestat] mean and std.dev: (3195.97, 2898.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20138) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591) [M::mem_pestat] mean and std.dev: (223.45, 112.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 741) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (814, 2768, 5629) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15259) [M::mem_pestat] mean and std.dev: (3431.68, 2893.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20074) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (427, 2239, 5795) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16531) [M::mem_pestat] mean and std.dev: (3245.56, 3071.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21899) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1863.457 CPU sec, 116.904 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (860, 400188, 660, 815) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (479, 2211, 5170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14552) [M::mem_pestat] mean and std.dev: (3103.84, 2934.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19243) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 201, 292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 592) [M::mem_pestat] mean and std.dev: (223.97, 112.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 742) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1021, 3274, 6371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 17071) [M::mem_pestat] mean and std.dev: (3845.36, 3051.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 22421) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (467, 1777, 4649) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13013) [M::mem_pestat] mean and std.dev: (2868.07, 2845.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17195) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1861.519 CPU sec, 116.840 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (817, 397183, 708, 833) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (571, 2095, 5582) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15604) [M::mem_pestat] mean and std.dev: (3166.63, 2966.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20615) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 202, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 601) [M::mem_pestat] mean and std.dev: (226.17, 115.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 754) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (873, 2795, 5638) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15168) [M::mem_pestat] mean and std.dev: (3387.78, 2801.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19933) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (477, 2052, 5263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14835) [M::mem_pestat] mean and std.dev: (3073.13, 2958.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19621) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1874.553 CPU sec, 117.613 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (870, 398040, 652, 856) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (498, 2281, 5653) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15963) [M::mem_pestat] mean and std.dev: (3301.97, 3010.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21118) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 202, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 601) [M::mem_pestat] mean and std.dev: (225.93, 114.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 754) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (877, 2887, 5854) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15808) [M::mem_pestat] mean and std.dev: (3603.29, 3013.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20785) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (536, 2267, 5366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15026) [M::mem_pestat] mean and std.dev: (3197.42, 2927.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19856) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1885.804 CPU sec, 118.387 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (804, 399943, 663, 859) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (477, 2107, 4945) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13881) [M::mem_pestat] mean and std.dev: (2975.29, 2813.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18349) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 201, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595) [M::mem_pestat] mean and std.dev: (224.51, 113.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 746) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (923, 3061, 6011) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16187) [M::mem_pestat] mean and std.dev: (3707.34, 3029.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21275) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (513, 2297, 5618) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15828) [M::mem_pestat] mean and std.dev: (3269.29, 3036.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20933) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1871.847 CPU sec, 117.453 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (833, 400615, 708, 877) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (585, 2365, 5609) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15657) [M::mem_pestat] mean and std.dev: (3282.24, 2997.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20681) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 202, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595) [M::mem_pestat] mean and std.dev: (224.33, 113.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 746) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (821, 2809, 5452) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14714) [M::mem_pestat] mean and std.dev: (3462.83, 2902.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19345) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (620, 2502, 5494) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15242) [M::mem_pestat] mean and std.dev: (3274.49, 2886.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20116) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1875.222 CPU sec, 117.622 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (836, 403073, 639, 811) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (533, 2360, 5552) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15590) [M::mem_pestat] mean and std.dev: (3290.37, 2999.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20609) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 202, 292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 592) [M::mem_pestat] mean and std.dev: (223.86, 112.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 742) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (788, 2815, 5877) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16055) [M::mem_pestat] mean and std.dev: (3574.52, 3088.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21144) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (578, 2015, 5276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14672) [M::mem_pestat] mean and std.dev: (3180.44, 2970.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19370) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1922.786 CPU sec, 120.602 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (727, 402324, 638, 782) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (458, 2002, 5469) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15491) [M::mem_pestat] mean and std.dev: (3176.02, 3097.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20502) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 192, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 553) [M::mem_pestat] mean and std.dev: (211.80, 103.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 692) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (806, 2905, 6283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 17237) [M::mem_pestat] mean and std.dev: (3683.67, 3072.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 22714) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (467, 1903, 4897) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13757) [M::mem_pestat] mean and std.dev: (2939.63, 2876.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18187) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 2314.496 CPU sec, 145.184 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (823, 393649, 598, 795) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (498, 2115, 5267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14805) [M::mem_pestat] mean and std.dev: (3194.53, 2984.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19574) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 195, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 575) [M::mem_pestat] mean and std.dev: (216.93, 108.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 721) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (998, 2680, 5740) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15224) [M::mem_pestat] mean and std.dev: (3499.69, 2909.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19966) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (464, 2016, 5513) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15611) [M::mem_pestat] mean and std.dev: (3157.06, 3007.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20660) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 2280.667 CPU sec, 143.042 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (865, 392642, 665, 795) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (428, 1923, 5478) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15578) [M::mem_pestat] mean and std.dev: (3088.45, 3001.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20628) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 199, 290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 590) [M::mem_pestat] mean and std.dev: (222.36, 112.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 740) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1072, 3243, 5915) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15601) [M::mem_pestat] mean and std.dev: (3707.20, 2871.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20444) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (488, 2168, 5611) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15857) [M::mem_pestat] mean and std.dev: (3212.80, 2984.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20980) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 2032.308 CPU sec, 127.490 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1053, 397604, 929, 1064) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (526, 2108, 5177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14479) [M::mem_pestat] mean and std.dev: (3076.97, 2874.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19130) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 179, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 559) [M::mem_pestat] mean and std.dev: (201.78, 108.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 705) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (897, 2904, 5867) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15807) [M::mem_pestat] mean and std.dev: (3558.02, 2955.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20777) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (455, 1824, 4890) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13760) [M::mem_pestat] mean and std.dev: (2926.68, 2912.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18195) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1528.857 CPU sec, 96.046 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1265, 394133, 1134, 1250) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (566, 2348, 5512) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15404) [M::mem_pestat] mean and std.dev: (3249.05, 2922.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20350) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 171, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 549) [M::mem_pestat] mean and std.dev: (194.84, 106.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 694) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (780, 2635, 5621) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15303) [M::mem_pestat] mean and std.dev: (3435.02, 2983.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20144) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (436, 1841, 5148) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14572) [M::mem_pestat] mean and std.dev: (3032.89, 2978.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19284) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1005.898 CPU sec, 63.333 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1338, 391393, 1084, 1354) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (424, 1805, 4783) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13501) [M::mem_pestat] mean and std.dev: (2917.48, 2889.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17860) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 173, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 562) [M::mem_pestat] mean and std.dev: (198.25, 109.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 711) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (718, 2677, 5876) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16192) [M::mem_pestat] mean and std.dev: (3491.06, 3003.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21350) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (460, 2055, 5124) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14452) [M::mem_pestat] mean and std.dev: (3065.10, 2938.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19116) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 930.789 CPU sec, 58.646 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1265, 391029, 1163, 1293) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (427, 1966, 5047) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14287) [M::mem_pestat] mean and std.dev: (3012.14, 2926.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18907) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 174, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 565) [M::mem_pestat] mean and std.dev: (199.18, 110.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 715) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (842, 2811, 5536) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14924) [M::mem_pestat] mean and std.dev: (3399.66, 2856.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19618) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (517, 2099, 5018) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14020) [M::mem_pestat] mean and std.dev: (3084.91, 2908.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18521) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 953.548 CPU sec, 60.066 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1236, 392898, 1060, 1348) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (479, 2034, 5203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14651) [M::mem_pestat] mean and std.dev: (3091.33, 2983.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19375) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 174, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 559) [M::mem_pestat] mean and std.dev: (197.95, 108.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 707) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (785, 2766, 5709) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15557) [M::mem_pestat] mean and std.dev: (3424.93, 2918.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20481) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (416, 1780, 5216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14816) [M::mem_pestat] mean and std.dev: (3003.59, 2972.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19616) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 946.400 CPU sec, 59.624 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1277, 391733, 1097, 1326) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (414, 1827, 4955) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14037) [M::mem_pestat] mean and std.dev: (2914.36, 2885.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18578) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 175, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 566) [M::mem_pestat] mean and std.dev: (200.11, 110.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 716) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (734, 2459, 5433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14831) [M::mem_pestat] mean and std.dev: (3341.95, 2924.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19530) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (428, 1634, 4870) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13754) [M::mem_pestat] mean and std.dev: (2887.58, 2925.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18196) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 927.117 CPU sec, 58.439 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1294, 389543, 1112, 1300) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (488, 2032, 4968) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13928) [M::mem_pestat] mean and std.dev: (3001.37, 2871.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18408) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 176, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 569) [M::mem_pestat] mean and std.dev: (200.96, 111.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 720) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (757, 2704, 5631) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15379) [M::mem_pestat] mean and std.dev: (3392.14, 2884.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20253) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (404, 1900, 4831) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13685) [M::mem_pestat] mean and std.dev: (2887.32, 2843.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18112) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 938.276 CPU sec, 59.439 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1250, 393045, 1110, 1265) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (475, 2077, 5463) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15439) [M::mem_pestat] mean and std.dev: (3127.53, 2946.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20427) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 175, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 566) [M::mem_pestat] mean and std.dev: (199.56, 110.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 716) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (678, 2715, 5646) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15582) [M::mem_pestat] mean and std.dev: (3397.43, 2943.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20550) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (438, 1921, 5080) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14364) [M::mem_pestat] mean and std.dev: (2995.65, 2930.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19006) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 918.426 CPU sec, 58.100 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1310, 391553, 1130, 1347) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (517, 2074, 5015) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14011) [M::mem_pestat] mean and std.dev: (3065.30, 2904.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18509) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 175, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 566) [M::mem_pestat] mean and std.dev: (200.03, 110.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 716) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (753, 2710, 6049) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16641) [M::mem_pestat] mean and std.dev: (3512.72, 2999.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21937) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (436, 1857, 4938) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13942) [M::mem_pestat] mean and std.dev: (2894.36, 2872.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18444) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 915.945 CPU sec, 57.704 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1335, 392495, 1069, 1275) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (447, 1905, 5294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14988) [M::mem_pestat] mean and std.dev: (3050.39, 2967.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19835) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 175, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 566) [M::mem_pestat] mean and std.dev: (199.46, 110.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 716) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (824, 2585, 5830) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15842) [M::mem_pestat] mean and std.dev: (3440.52, 2925.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20848) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (418, 1908, 4821) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13627) [M::mem_pestat] mean and std.dev: (2874.25, 2803.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18030) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 917.186 CPU sec, 57.820 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1224, 395620, 1085, 1237) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (515, 2012, 5488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15434) [M::mem_pestat] mean and std.dev: (3162.45, 3015.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20407) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 173, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 553) [M::mem_pestat] mean and std.dev: (196.53, 107.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 699) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (700, 2693, 6031) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16693) [M::mem_pestat] mean and std.dev: (3467.02, 3039.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 22024) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (470, 1887, 5178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14594) [M::mem_pestat] mean and std.dev: (3019.47, 2899.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19302) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 930.084 CPU sec, 58.589 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1357, 397395, 1092, 1285) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (473, 2036, 5051) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14207) [M::mem_pestat] mean and std.dev: (2999.93, 2857.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18785) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 173, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 553) [M::mem_pestat] mean and std.dev: (196.29, 107.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 699) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (733, 2775, 5466) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14932) [M::mem_pestat] mean and std.dev: (3427.76, 2958.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19665) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (465, 1978, 5031) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14163) [M::mem_pestat] mean and std.dev: (2991.02, 2847.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18729) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 913.006 CPU sec, 57.794 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1256, 395023, 996, 1381) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (455, 2054, 5405) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15305) [M::mem_pestat] mean and std.dev: (3120.68, 3003.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20255) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 174, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 557) [M::mem_pestat] mean and std.dev: (197.99, 108.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 704) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (745, 2622, 5719) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15667) [M::mem_pestat] mean and std.dev: (3393.35, 2913.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20641) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (430, 1977, 5348) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15184) [M::mem_pestat] mean and std.dev: (3098.41, 3003.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20102) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 935.802 CPU sec, 58.893 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1307, 393054, 1174, 1258) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (497, 2064, 5304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14918) [M::mem_pestat] mean and std.dev: (3087.84, 2920.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19725) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 174, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 562) [M::mem_pestat] mean and std.dev: (198.43, 109.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 711) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (896, 3048, 5980) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16148) [M::mem_pestat] mean and std.dev: (3621.35, 2947.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21232) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (458, 1888, 5367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15185) [M::mem_pestat] mean and std.dev: (3070.13, 2962.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20094) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 931.865 CPU sec, 58.700 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1314, 395609, 1121, 1350) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (429, 1918, 5101) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14445) [M::mem_pestat] mean and std.dev: (3016.83, 2939.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19117) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 174, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 559) [M::mem_pestat] mean and std.dev: (197.71, 108.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 707) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (703, 2911, 5993) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16573) [M::mem_pestat] mean and std.dev: (3577.69, 3051.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21863) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (424, 2010, 5043) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14281) [M::mem_pestat] mean and std.dev: (3006.62, 2913.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18900) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 919.290 CPU sec, 57.909 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1245, 396418, 1088, 1315) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (434, 2027, 5345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15167) [M::mem_pestat] mean and std.dev: (3075.42, 2929.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20078) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 173, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 553) [M::mem_pestat] mean and std.dev: (196.25, 106.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 699) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (808, 2725, 5734) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15586) [M::mem_pestat] mean and std.dev: (3460.95, 2938.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20512) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (477, 2085, 5271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14859) [M::mem_pestat] mean and std.dev: (3103.59, 2968.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19653) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 945.405 CPU sec, 59.568 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1271, 397940, 1117, 1308) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (417, 1753, 5050) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14316) [M::mem_pestat] mean and std.dev: (2939.23, 2945.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18949) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 172, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 550) [M::mem_pestat] mean and std.dev: (195.58, 106.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 695) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (755, 2851, 5973) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16409) [M::mem_pestat] mean and std.dev: (3525.23, 2982.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21627) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (440, 2011, 5250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14870) [M::mem_pestat] mean and std.dev: (3096.22, 2933.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19680) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 938.941 CPU sec, 59.156 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1279, 392769, 1149, 1330) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (495, 1887, 4826) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13488) [M::mem_pestat] mean and std.dev: (2971.23, 2849.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17819) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 174, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 560) [M::mem_pestat] mean and std.dev: (198.30, 109.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 708) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (760, 2656, 5660) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15460) [M::mem_pestat] mean and std.dev: (3419.90, 2977.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20360) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (403, 1835, 4921) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13957) [M::mem_pestat] mean and std.dev: (2960.14, 2947.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18475) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 963.663 CPU sec, 60.682 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1260, 389342, 1121, 1324) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (500, 2237, 5420) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15260) [M::mem_pestat] mean and std.dev: (3159.50, 2925.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20180) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 175, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 569) [M::mem_pestat] mean and std.dev: (200.08, 111.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 720) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (834, 2876, 5899) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16029) [M::mem_pestat] mean and std.dev: (3556.42, 2959.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21094) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (430, 1793, 5063) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14329) [M::mem_pestat] mean and std.dev: (3003.64, 3015.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18962) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 950.770 CPU sec, 59.903 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1253, 391002, 1112, 1370) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (441, 1675, 4870) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13728) [M::mem_pestat] mean and std.dev: (2932.84, 2911.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18157) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 175, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 569) [M::mem_pestat] mean and std.dev: (200.11, 111.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 720) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (849, 2775, 5874) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15924) [M::mem_pestat] mean and std.dev: (3542.37, 2974.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20949) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (506, 2159, 5396) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15176) [M::mem_pestat] mean and std.dev: (3172.04, 3011.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20066) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 937.506 CPU sec, 59.027 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1280, 390811, 1092, 1339) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (516, 2459, 5460) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15348) [M::mem_pestat] mean and std.dev: (3230.03, 2939.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20292) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 175, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 566) [M::mem_pestat] mean and std.dev: (199.66, 110.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 716) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (936, 2717, 5652) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15084) [M::mem_pestat] mean and std.dev: (3529.70, 2989.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19800) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (436, 1969, 5018) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14182) [M::mem_pestat] mean and std.dev: (2952.87, 2877.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18764) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 935.424 CPU sec, 58.972 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1226, 390377, 1153, 1249) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (453, 1986, 4938) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13908) [M::mem_pestat] mean and std.dev: (2951.96, 2850.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18393) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 174, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 565) [M::mem_pestat] mean and std.dev: (198.89, 110.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 715) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (728, 2564, 5785) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15899) [M::mem_pestat] mean and std.dev: (3445.40, 3003.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20956) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (413, 2037, 4960) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14054) [M::mem_pestat] mean and std.dev: (3029.41, 2924.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18601) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 983.517 CPU sec, 61.881 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1292, 392552, 1135, 1357) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (552, 2092, 5089) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14163) [M::mem_pestat] mean and std.dev: (3042.18, 2840.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18700) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 172, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 556) [M::mem_pestat] mean and std.dev: (196.81, 108.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 703) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (949, 3066, 5895) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15787) [M::mem_pestat] mean and std.dev: (3648.09, 2952.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20733) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (474, 2085, 5303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14961) [M::mem_pestat] mean and std.dev: (3075.55, 2885.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19790) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1017.062 CPU sec, 63.979 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1345, 393643, 1088, 1310) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (507, 2048, 5162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14472) [M::mem_pestat] mean and std.dev: (3088.06, 2928.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19127) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 173, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 562) [M::mem_pestat] mean and std.dev: (198.13, 109.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 711) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (897, 2882, 6028) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16290) [M::mem_pestat] mean and std.dev: (3605.74, 2978.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21421) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (429, 2011, 4907) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13863) [M::mem_pestat] mean and std.dev: (2981.95, 2898.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18341) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 935.426 CPU sec, 58.851 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1296, 393537, 1098, 1305) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (443, 2022, 5091) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14387) [M::mem_pestat] mean and std.dev: (3041.38, 2955.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19035) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 173, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 562) [M::mem_pestat] mean and std.dev: (198.21, 109.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 711) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (725, 2671, 5649) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15497) [M::mem_pestat] mean and std.dev: (3426.65, 2985.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20421) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (475, 1949, 4988) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14014) [M::mem_pestat] mean and std.dev: (3015.91, 2876.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18527) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 931.811 CPU sec, 58.709 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1298, 394534, 1052, 1319) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (507, 2167, 5240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14706) [M::mem_pestat] mean and std.dev: (3161.40, 2934.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19439) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 173, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 556) [M::mem_pestat] mean and std.dev: (197.24, 108.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 703) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (835, 3088, 5923) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16099) [M::mem_pestat] mean and std.dev: (3618.98, 2947.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21187) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (446, 2050, 5181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14651) [M::mem_pestat] mean and std.dev: (3078.21, 2923.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19386) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 940.675 CPU sec, 59.242 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1246, 395278, 1099, 1239) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (456, 1883, 5161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14571) [M::mem_pestat] mean and std.dev: (3020.32, 2882.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19276) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 173, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 556) [M::mem_pestat] mean and std.dev: (197.23, 108.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 703) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (750, 2501, 5530) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15090) [M::mem_pestat] mean and std.dev: (3359.61, 2940.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19870) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (454, 1793, 4913) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13831) [M::mem_pestat] mean and std.dev: (2928.25, 2901.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18290) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 952.008 CPU sec, 59.884 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1344, 394294, 1055, 1289) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (501, 1938, 5012) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14034) [M::mem_pestat] mean and std.dev: (3028.24, 2926.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18545) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 174, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 562) [M::mem_pestat] mean and std.dev: (198.27, 109.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 711) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (833, 2706, 5768) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15638) [M::mem_pestat] mean and std.dev: (3517.94, 2949.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20573) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (471, 1836, 4864) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13650) [M::mem_pestat] mean and std.dev: (2964.32, 2886.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18043) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 942.806 CPU sec, 59.340 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1274, 392171, 1133, 1330) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (411, 1920, 5031) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14271) [M::mem_pestat] mean and std.dev: (2938.70, 2858.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18891) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 172, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 558) [M::mem_pestat] mean and std.dev: (196.96, 109.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 706) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (731, 2454, 5441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14861) [M::mem_pestat] mean and std.dev: (3326.20, 2944.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19571) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (457, 1976, 4972) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14002) [M::mem_pestat] mean and std.dev: (3016.47, 2914.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18517) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1045.028 CPU sec, 65.791 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1354, 393374, 1067, 1312) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (509, 2159, 5273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14801) [M::mem_pestat] mean and std.dev: (3072.61, 2866.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19565) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 174, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 566) [M::mem_pestat] mean and std.dev: (199.33, 110.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 716) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (736, 2584, 5716) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15676) [M::mem_pestat] mean and std.dev: (3374.78, 2912.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20656) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (445, 1912, 5078) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14344) [M::mem_pestat] mean and std.dev: (2992.06, 2888.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18977) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 975.151 CPU sec, 61.392 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1179, 399092, 1069, 1207) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (455, 2132, 5120) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14450) [M::mem_pestat] mean and std.dev: (3066.63, 2880.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19115) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 166, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 528) [M::mem_pestat] mean and std.dev: (188.63, 102.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 667) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (698, 2559, 5897) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16295) [M::mem_pestat] mean and std.dev: (3444.57, 3026.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21494) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (453, 1840, 4911) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13827) [M::mem_pestat] mean and std.dev: (2928.69, 2823.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18285) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1207.454 CPU sec, 75.887 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1327, 391837, 1057, 1250) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (470, 1962, 5042) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14186) [M::mem_pestat] mean and std.dev: (3085.22, 2994.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18758) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 171, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 555) [M::mem_pestat] mean and std.dev: (195.98, 108.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 702) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (752, 2474, 5524) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15068) [M::mem_pestat] mean and std.dev: (3362.48, 2918.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19840) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (356, 1801, 5187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14849) [M::mem_pestat] mean and std.dev: (3002.17, 3000.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19680) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1116.233 CPU sec, 70.263 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1314, 390393, 1093, 1325) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (512, 2102, 5188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14540) [M::mem_pestat] mean and std.dev: (3109.93, 2946.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19216) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 173, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 562) [M::mem_pestat] mean and std.dev: (198.17, 110.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 711) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (729, 2755, 5967) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16443) [M::mem_pestat] mean and std.dev: (3522.62, 3009.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21681) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (470, 1823, 5252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14816) [M::mem_pestat] mean and std.dev: (3038.52, 2975.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19598) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1046.185 CPU sec, 65.869 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1233, 387828, 1132, 1301) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (530, 2199, 5252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14696) [M::mem_pestat] mean and std.dev: (3111.36, 2898.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19418) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 175, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 572) [M::mem_pestat] mean and std.dev: (201.14, 112.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 724) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (817, 2948, 5816) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15814) [M::mem_pestat] mean and std.dev: (3570.71, 3000.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20813) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (449, 2023, 5071) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14315) [M::mem_pestat] mean and std.dev: (3019.55, 2923.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18937) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1001.393 CPU sec, 63.180 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1280, 388574, 1119, 1310) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (436, 1974, 5155) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14593) [M::mem_pestat] mean and std.dev: (3045.14, 2930.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19312) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 174, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 571) [M::mem_pestat] mean and std.dev: (200.29, 112.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 723) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (841, 2660, 5823) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15787) [M::mem_pestat] mean and std.dev: (3449.86, 2940.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20769) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (477, 2124, 5286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14904) [M::mem_pestat] mean and std.dev: (3126.28, 2961.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19713) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1035.749 CPU sec, 65.121 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1245, 387520, 1194, 1345) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (558, 1993, 4991) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13857) [M::mem_pestat] mean and std.dev: (2983.80, 2790.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18290) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 176, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 575) [M::mem_pestat] mean and std.dev: (201.83, 112.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 728) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (820, 2644, 5742) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15586) [M::mem_pestat] mean and std.dev: (3436.51, 2961.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20508) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (500, 2168, 5216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14648) [M::mem_pestat] mean and std.dev: (3063.57, 2813.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19364) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 957.437 CPU sec, 60.251 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1360, 389135, 1093, 1297) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (493, 1948, 5141) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14437) [M::mem_pestat] mean and std.dev: (3033.87, 2931.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19085) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 175, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 572) [M::mem_pestat] mean and std.dev: (201.35, 112.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 724) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (770, 2547, 5793) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15839) [M::mem_pestat] mean and std.dev: (3415.21, 2960.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20862) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (372, 1668, 5103) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14565) [M::mem_pestat] mean and std.dev: (2900.06, 2928.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19296) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 947.252 CPU sec, 59.607 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1264, 389831, 1059, 1389) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (442, 1987, 5601) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15919) [M::mem_pestat] mean and std.dev: (3147.02, 3027.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21078) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 175, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 569) [M::mem_pestat] mean and std.dev: (200.30, 111.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 720) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (864, 2630, 5619) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15129) [M::mem_pestat] mean and std.dev: (3445.08, 2914.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19884) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (426, 1863, 4952) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14004) [M::mem_pestat] mean and std.dev: (2959.98, 2941.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18530) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 945.152 CPU sec, 59.523 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1308, 390840, 1065, 1258) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (478, 2081, 4868) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13648) [M::mem_pestat] mean and std.dev: (3026.63, 2878.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18038) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 175, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 569) [M::mem_pestat] mean and std.dev: (200.10, 111.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 720) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (694, 2505, 5538) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15226) [M::mem_pestat] mean and std.dev: (3352.82, 2956.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20070) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (396, 1838, 4924) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13980) [M::mem_pestat] mean and std.dev: (2935.76, 2910.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18508) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 947.262 CPU sec, 59.658 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1325, 390370, 1123, 1327) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (497, 2102, 5389) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15173) [M::mem_pestat] mean and std.dev: (3120.78, 2942.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20065) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 175, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 569) [M::mem_pestat] mean and std.dev: (200.34, 111.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 720) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (824, 2936, 5752) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15608) [M::mem_pestat] mean and std.dev: (3503.28, 2944.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20536) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (430, 1942, 5384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15292) [M::mem_pestat] mean and std.dev: (3060.93, 2972.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20246) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 940.926 CPU sec, 59.249 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1332, 389641, 1172, 1286) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (583, 2355, 5542) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15460) [M::mem_pestat] mean and std.dev: (3245.90, 2922.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20419) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 174, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 568) [M::mem_pestat] mean and std.dev: (199.67, 111.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 719) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (856, 2646, 5858) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15862) [M::mem_pestat] mean and std.dev: (3483.71, 2966.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20864) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (412, 1957, 5150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14626) [M::mem_pestat] mean and std.dev: (3013.41, 2965.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19364) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 999.802 CPU sec, 62.948 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1321, 389698, 1129, 1300) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (509, 2034, 4975) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13907) [M::mem_pestat] mean and std.dev: (3043.61, 2881.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18373) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 175, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 569) [M::mem_pestat] mean and std.dev: (200.65, 111.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 720) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (679, 2404, 5699) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15739) [M::mem_pestat] mean and std.dev: (3363.64, 2991.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20759) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (497, 2028, 5049) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14153) [M::mem_pestat] mean and std.dev: (3031.56, 2876.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18705) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 924.327 CPU sec, 58.259 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1286, 392353, 1135, 1225) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (502, 2168, 5248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14740) [M::mem_pestat] mean and std.dev: (3111.56, 2890.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19486) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 173, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 562) [M::mem_pestat] mean and std.dev: (198.07, 109.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 711) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (730, 2822, 6000) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16540) [M::mem_pestat] mean and std.dev: (3536.35, 3010.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21810) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (419, 2080, 5132) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14558) [M::mem_pestat] mean and std.dev: (3052.64, 2889.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19271) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 934.914 CPU sec, 58.845 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1270, 394341, 1089, 1315) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (489, 2060, 4796) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13410) [M::mem_pestat] mean and std.dev: (2977.73, 2854.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17717) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 173, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 556) [M::mem_pestat] mean and std.dev: (197.11, 108.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 703) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (759, 2414, 5373) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14601) [M::mem_pestat] mean and std.dev: (3270.91, 2908.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19215) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (453, 1940, 5353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15153) [M::mem_pestat] mean and std.dev: (3071.86, 2979.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20053) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 937.416 CPU sec, 59.019 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1300, 393847, 1110, 1297) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (431, 1992, 5100) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14438) [M::mem_pestat] mean and std.dev: (3016.18, 2889.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19107) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 173, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 559) [M::mem_pestat] mean and std.dev: (197.48, 109.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 707) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (714, 2723, 5555) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15237) [M::mem_pestat] mean and std.dev: (3398.94, 2930.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20078) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (409, 1981, 4974) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14104) [M::mem_pestat] mean and std.dev: (2980.29, 2903.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18669) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 930.076 CPU sec, 58.530 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1217, 394242, 1064, 1310) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (414, 1945, 5179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14709) [M::mem_pestat] mean and std.dev: (3047.26, 2955.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19474) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 173, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 559) [M::mem_pestat] mean and std.dev: (197.50, 108.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 707) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (793, 2569, 5557) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15085) [M::mem_pestat] mean and std.dev: (3379.01, 2926.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19849) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (412, 2077, 5163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14665) [M::mem_pestat] mean and std.dev: (3089.34, 2946.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19416) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 939.517 CPU sec, 59.199 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1256, 391811, 1111, 1276) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (469, 1893, 4779) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13399) [M::mem_pestat] mean and std.dev: (2940.48, 2864.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17709) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 174, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 565) [M::mem_pestat] mean and std.dev: (198.84, 110.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 715) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (775, 2753, 5529) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15037) [M::mem_pestat] mean and std.dev: (3362.74, 2822.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19791) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (433, 1885, 5079) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14371) [M::mem_pestat] mean and std.dev: (3000.05, 2946.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19017) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 964.510 CPU sec, 60.686 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1255, 392642, 1142, 1317) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (442, 1729, 4841) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13639) [M::mem_pestat] mean and std.dev: (2919.20, 2887.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18038) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 174, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 565) [M::mem_pestat] mean and std.dev: (198.92, 110.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 715) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (847, 2717, 5868) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15910) [M::mem_pestat] mean and std.dev: (3516.28, 3005.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20931) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (464, 2030, 5015) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14117) [M::mem_pestat] mean and std.dev: (3025.38, 2913.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18668) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 945.030 CPU sec, 59.540 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1345, 396051, 1019, 1296) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (521, 2215, 5200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14558) [M::mem_pestat] mean and std.dev: (3148.78, 2942.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19237) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 173, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 556) [M::mem_pestat] mean and std.dev: (196.99, 108.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 703) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (782, 2490, 5721) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15599) [M::mem_pestat] mean and std.dev: (3451.15, 2968.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20538) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (435, 1768, 5032) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14226) [M::mem_pestat] mean and std.dev: (2949.87, 2957.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18823) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 951.648 CPU sec, 59.936 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1250, 393514, 1075, 1266) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (483, 2065, 5784) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16386) [M::mem_pestat] mean and std.dev: (3232.65, 3056.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21687) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 173, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 562) [M::mem_pestat] mean and std.dev: (197.91, 109.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 711) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (765, 2895, 6095) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16755) [M::mem_pestat] mean and std.dev: (3595.55, 3031.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 22085) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (404, 1592, 5064) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14384) [M::mem_pestat] mean and std.dev: (2924.39, 2954.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19044) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1018.231 CPU sec, 64.101 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1269, 401019, 1021, 1234) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (474, 2054, 4944) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13884) [M::mem_pestat] mean and std.dev: (3042.83, 2887.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18354) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 165, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 522) [M::mem_pestat] mean and std.dev: (187.20, 100.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 659) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (838, 2583, 5658) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15298) [M::mem_pestat] mean and std.dev: (3396.10, 2863.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20118) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (373, 1855, 4804) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13666) [M::mem_pestat] mean and std.dev: (2884.95, 2859.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18097) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1222.170 CPU sec, 76.839 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1192, 395616, 1067, 1206) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (529, 2196, 5252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14698) [M::mem_pestat] mean and std.dev: (3122.65, 2886.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19421) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 169, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 539) [M::mem_pestat] mean and std.dev: (191.94, 105.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 681) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (813, 2505, 5647) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15315) [M::mem_pestat] mean and std.dev: (3330.41, 2893.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20149) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (447, 2002, 4985) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14061) [M::mem_pestat] mean and std.dev: (3056.17, 2951.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18599) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1145.104 CPU sec, 72.024 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1272, 392176, 1126, 1272) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (488, 2026, 5504) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15536) [M::mem_pestat] mean and std.dev: (3161.30, 3017.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20552) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 172, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 555) [M::mem_pestat] mean and std.dev: (196.14, 108.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 702) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (725, 2787, 5945) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16385) [M::mem_pestat] mean and std.dev: (3555.96, 3038.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21605) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (386, 1973, 5394) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15410) [M::mem_pestat] mean and std.dev: (3021.95, 2943.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20418) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1056.250 CPU sec, 66.516 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1281, 391456, 1067, 1335) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (495, 2156, 5020) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14070) [M::mem_pestat] mean and std.dev: (3077.58, 2890.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18595) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 173, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 562) [M::mem_pestat] mean and std.dev: (198.14, 110.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 711) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (735, 2658, 5780) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15870) [M::mem_pestat] mean and std.dev: (3429.95, 2968.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20915) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (417, 1945, 5172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14682) [M::mem_pestat] mean and std.dev: (2967.95, 2882.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19437) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1009.357 CPU sec, 63.559 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1276, 393588, 1091, 1362) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (427, 1637, 4952) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14002) [M::mem_pestat] mean and std.dev: (2903.90, 2952.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18527) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 173, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 559) [M::mem_pestat] mean and std.dev: (197.58, 109.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 707) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (871, 2687, 5666) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15256) [M::mem_pestat] mean and std.dev: (3452.82, 2875.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20051) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (406, 1768, 5172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14704) [M::mem_pestat] mean and std.dev: (2987.65, 2974.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19470) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 988.883 CPU sec, 62.286 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1294, 392315, 1138, 1349) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (504, 1977, 5156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14460) [M::mem_pestat] mean and std.dev: (2988.16, 2827.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19112) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 174, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 559) [M::mem_pestat] mean and std.dev: (197.85, 109.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 707) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (784, 2554, 5638) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15346) [M::mem_pestat] mean and std.dev: (3389.36, 2954.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20200) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (432, 1921, 5338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15150) [M::mem_pestat] mean and std.dev: (3068.47, 2964.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20056) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 969.231 CPU sec, 61.233 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1188, 393170, 1153, 1287) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (465, 1901, 5031) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14163) [M::mem_pestat] mean and std.dev: (3061.49, 2981.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18729) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 174, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 557) [M::mem_pestat] mean and std.dev: (197.84, 108.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 704) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (883, 2983, 6133) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16633) [M::mem_pestat] mean and std.dev: (3627.06, 2998.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21883) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (524, 2128, 5320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14912) [M::mem_pestat] mean and std.dev: (3158.31, 2956.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19708) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 943.641 CPU sec, 59.390 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1271, 392753, 1117, 1250) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (554, 2214, 5601) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15695) [M::mem_pestat] mean and std.dev: (3261.43, 3031.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20742) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 174, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 563) [M::mem_pestat] mean and std.dev: (198.96, 110.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 712) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (674, 2592, 5695) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15737) [M::mem_pestat] mean and std.dev: (3387.14, 2947.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20758) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (507, 1858, 5081) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14229) [M::mem_pestat] mean and std.dev: (3020.02, 2883.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18803) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 946.309 CPU sec, 59.515 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1245, 392888, 1098, 1291) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (448, 1850, 5149) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14551) [M::mem_pestat] mean and std.dev: (2986.32, 2901.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19252) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 174, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 562) [M::mem_pestat] mean and std.dev: (198.42, 109.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 711) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (810, 2727, 5785) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15735) [M::mem_pestat] mean and std.dev: (3542.06, 3009.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20710) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (433, 1874, 4952) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13990) [M::mem_pestat] mean and std.dev: (2977.88, 2920.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18509) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 935.362 CPU sec, 58.897 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1369, 389088, 1104, 1312) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (509, 2088, 5311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14915) [M::mem_pestat] mean and std.dev: (3143.42, 2949.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19717) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 176, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 575) [M::mem_pestat] mean and std.dev: (201.59, 112.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 728) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (685, 2436, 5369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14737) [M::mem_pestat] mean and std.dev: (3252.52, 2850.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19421) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (496, 2053, 5086) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14266) [M::mem_pestat] mean and std.dev: (3101.80, 2952.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18856) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 943.131 CPU sec, 59.409 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1235, 392967, 1127, 1307) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (455, 2114, 5139) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14507) [M::mem_pestat] mean and std.dev: (3052.37, 2943.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19191) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 174, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 563) [M::mem_pestat] mean and std.dev: (198.77, 109.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 712) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (791, 2700, 5820) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15878) [M::mem_pestat] mean and std.dev: (3488.40, 2964.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20907) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (461, 1816, 4988) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14042) [M::mem_pestat] mean and std.dev: (2993.08, 2977.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18569) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 917.049 CPU sec, 57.927 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1330, 392113, 1072, 1280) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (470, 1859, 5050) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14210) [M::mem_pestat] mean and std.dev: (2989.53, 2887.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18790) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 175, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 563) [M::mem_pestat] mean and std.dev: (198.94, 109.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 712) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (699, 2486, 5629) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15489) [M::mem_pestat] mean and std.dev: (3406.01, 3024.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20419) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (470, 1746, 5134) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14462) [M::mem_pestat] mean and std.dev: (2987.76, 2970.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19126) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 916.978 CPU sec, 57.794 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1361, 394711, 1119, 1349) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (490, 2193, 5290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14890) [M::mem_pestat] mean and std.dev: (3120.05, 2908.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19690) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 173, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 556) [M::mem_pestat] mean and std.dev: (197.23, 108.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 703) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (678, 2490, 5756) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15912) [M::mem_pestat] mean and std.dev: (3354.17, 2987.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20990) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (455, 2142, 5299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14987) [M::mem_pestat] mean and std.dev: (3102.24, 2936.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19831) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 919.853 CPU sec, 57.922 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1287, 394833, 1101, 1307) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (479, 2263, 5406) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15260) [M::mem_pestat] mean and std.dev: (3198.18, 2978.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20187) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 173, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 556) [M::mem_pestat] mean and std.dev: (197.21, 108.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 703) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (825, 2657, 5703) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15459) [M::mem_pestat] mean and std.dev: (3463.17, 2984.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20337) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (404, 1943, 4963) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14081) [M::mem_pestat] mean and std.dev: (2918.53, 2840.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18640) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 917.825 CPU sec, 57.804 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1292, 395907, 1093, 1300) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (495, 2200, 5152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14466) [M::mem_pestat] mean and std.dev: (3117.00, 2942.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19123) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 173, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 556) [M::mem_pestat] mean and std.dev: (196.97, 107.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 703) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (665, 2393, 5405) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14885) [M::mem_pestat] mean and std.dev: (3267.44, 2902.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19625) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (466, 1935, 5113) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14407) [M::mem_pestat] mean and std.dev: (3039.98, 2930.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19054) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 918.595 CPU sec, 57.875 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1304, 390292, 1169, 1326) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (493, 2049, 5106) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14332) [M::mem_pestat] mean and std.dev: (3107.90, 2934.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18945) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 175, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 566) [M::mem_pestat] mean and std.dev: (199.85, 110.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 716) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (764, 2734, 5933) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16271) [M::mem_pestat] mean and std.dev: (3499.69, 2994.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21440) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (454, 1967, 5144) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14524) [M::mem_pestat] mean and std.dev: (3023.33, 2930.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19214) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 955.999 CPU sec, 60.180 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1299, 395493, 1053, 1346) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (495, 2003, 5235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14715) [M::mem_pestat] mean and std.dev: (3060.61, 2938.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19455) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 173, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 556) [M::mem_pestat] mean and std.dev: (197.11, 108.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 703) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (767, 3016, 6087) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16727) [M::mem_pestat] mean and std.dev: (3591.26, 2981.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 22047) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (448, 1841, 5111) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14437) [M::mem_pestat] mean and std.dev: (2978.29, 2902.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19100) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 934.799 CPU sec, 58.874 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1286, 395355, 1131, 1333) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (472, 2270, 5194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14638) [M::mem_pestat] mean and std.dev: (3104.50, 2908.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19360) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 174, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 556) [M::mem_pestat] mean and std.dev: (197.22, 108.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 703) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (678, 2493, 5720) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15804) [M::mem_pestat] mean and std.dev: (3377.80, 2970.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20846) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (447, 1982, 5342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15132) [M::mem_pestat] mean and std.dev: (3030.21, 2948.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20027) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 924.269 CPU sec, 58.255 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1321, 397239, 1127, 1216) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (513, 2122, 5453) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15333) [M::mem_pestat] mean and std.dev: (3161.70, 2965.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20273) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 173, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 553) [M::mem_pestat] mean and std.dev: (196.44, 107.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 699) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (815, 2566, 5658) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15344) [M::mem_pestat] mean and std.dev: (3405.42, 2915.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20187) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (428, 2002, 5284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14996) [M::mem_pestat] mean and std.dev: (3054.40, 2900.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19852) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 949.090 CPU sec, 59.794 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1219, 402193, 1061, 1149) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (397, 1919, 5142) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14632) [M::mem_pestat] mean and std.dev: (2980.15, 2921.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19377) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 165, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 522) [M::mem_pestat] mean and std.dev: (186.95, 100.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 659) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (789, 2366, 5266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14220) [M::mem_pestat] mean and std.dev: (3219.83, 2831.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18697) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (359, 1609, 4821) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13745) [M::mem_pestat] mean and std.dev: (2832.64, 2909.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18207) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1184.114 CPU sec, 74.452 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1243, 395090, 1115, 1284) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (443, 1810, 5052) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14270) [M::mem_pestat] mean and std.dev: (2962.23, 2876.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18879) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 169, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 539) [M::mem_pestat] mean and std.dev: (191.96, 105.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 681) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (796, 2581, 5718) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15562) [M::mem_pestat] mean and std.dev: (3383.74, 2909.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20484) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (461, 2004, 4953) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13937) [M::mem_pestat] mean and std.dev: (3026.69, 2902.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18429) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1131.643 CPU sec, 71.182 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1243, 392583, 1046, 1296) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (462, 1873, 5234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14778) [M::mem_pestat] mean and std.dev: (3008.52, 2911.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19550) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 171, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 558) [M::mem_pestat] mean and std.dev: (196.41, 109.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 706) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (866, 2578, 5869) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15875) [M::mem_pestat] mean and std.dev: (3468.48, 2961.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20878) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (438, 2141, 5310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15054) [M::mem_pestat] mean and std.dev: (3099.24, 2972.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19926) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 978.625 CPU sec, 61.594 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1119, 422665, 876, 1125) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (471, 2061, 5300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14958) [M::mem_pestat] mean and std.dev: (3104.49, 2938.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19787) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 143, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 441) [M::mem_pestat] mean and std.dev: (161.35, 84.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 555) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (724, 2699, 5409) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14779) [M::mem_pestat] mean and std.dev: (3366.61, 2907.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19464) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (355, 1813, 4647) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13231) [M::mem_pestat] mean and std.dev: (2788.02, 2802.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17523) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1110.358 CPU sec, 69.838 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1145, 414867, 1023, 1196) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (424, 1974, 5461) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15535) [M::mem_pestat] mean and std.dev: (3123.38, 3004.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20572) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (102, 148, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 462) [M::mem_pestat] mean and std.dev: (167.45, 89.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 582) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (734, 2700, 5637) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15443) [M::mem_pestat] mean and std.dev: (3420.93, 2949.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20346) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (371, 1755, 5029) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14345) [M::mem_pestat] mean and std.dev: (2872.76, 2898.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19003) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 941.633 CPU sec, 59.261 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1238, 411441, 1023, 1237) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (493, 2329, 5496) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15502) [M::mem_pestat] mean and std.dev: (3241.68, 2959.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20505) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (102, 150, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (170.20, 92.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (676, 2469, 5710) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15778) [M::mem_pestat] mean and std.dev: (3379.83, 2992.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20812) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (452, 1927, 5073) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14315) [M::mem_pestat] mean and std.dev: (3012.96, 2895.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18936) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 788.130 CPU sec, 49.740 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1205, 410242, 1007, 1331) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (442, 2085, 5275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14941) [M::mem_pestat] mean and std.dev: (3120.52, 2967.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19774) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (102, 150, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480) [M::mem_pestat] mean and std.dev: (171.46, 93.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (635, 2468, 5862) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16316) [M::mem_pestat] mean and std.dev: (3378.47, 3031.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21543) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (428, 2021, 4998) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14138) [M::mem_pestat] mean and std.dev: (3006.44, 2945.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18708) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 892.388 CPU sec, 56.257 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1220, 409914, 976, 1281) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (465, 2041, 5249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14817) [M::mem_pestat] mean and std.dev: (3104.81, 2949.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19601) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (103, 151, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (171.76, 93.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 603) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (755, 2710, 5766) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15788) [M::mem_pestat] mean and std.dev: (3438.66, 2967.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20799) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (401, 1769, 4963) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14087) [M::mem_pestat] mean and std.dev: (2936.60, 2897.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18649) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 867.440 CPU sec, 54.677 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1168, 412567, 974, 1298) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (468, 1970, 5537) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15675) [M::mem_pestat] mean and std.dev: (3183.38, 3018.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20744) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (103, 150, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (170.83, 92.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 599) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (775, 2952, 6116) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16798) [M::mem_pestat] mean and std.dev: (3579.96, 3067.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 22139) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (409, 1840, 5162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14668) [M::mem_pestat] mean and std.dev: (3010.62, 2983.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19421) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 848.366 CPU sec, 53.491 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1198, 411318, 1016, 1209) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (448, 1897, 5195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14689) [M::mem_pestat] mean and std.dev: (3004.02, 2884.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19436) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (103, 150, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (171.64, 93.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 603) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (792, 2548, 5861) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15999) [M::mem_pestat] mean and std.dev: (3450.68, 2998.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21068) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (441, 1949, 5071) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14331) [M::mem_pestat] mean and std.dev: (2962.46, 2876.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18961) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 851.645 CPU sec, 53.696 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1156, 409881, 1047, 1321) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (431, 2172, 5469) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15545) [M::mem_pestat] mean and std.dev: (3158.70, 3023.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20583) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (103, 151, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (172.30, 93.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 607) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (792, 2605, 5847) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15957) [M::mem_pestat] mean and std.dev: (3477.58, 3021.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21012) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (458, 2129, 5219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14741) [M::mem_pestat] mean and std.dev: (3093.63, 2941.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19502) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 838.960 CPU sec, 52.882 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1167, 407249, 1061, 1300) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (575, 2315, 5759) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16127) [M::mem_pestat] mean and std.dev: (3345.91, 3037.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21311) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (103, 152, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (173.46, 95.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 615) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (665, 2774, 5878) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16304) [M::mem_pestat] mean and std.dev: (3515.21, 3050.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21517) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (399, 1773, 4886) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13860) [M::mem_pestat] mean and std.dev: (2936.01, 2933.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18347) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 836.850 CPU sec, 52.723 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1203, 410341, 1029, 1279) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (442, 2029, 5274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14938) [M::mem_pestat] mean and std.dev: (3047.24, 2910.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19770) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (103, 151, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (172.35, 93.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 607) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (865, 2997, 5652) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15226) [M::mem_pestat] mean and std.dev: (3542.60, 2938.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20013) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (396, 1749, 5102) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14514) [M::mem_pestat] mean and std.dev: (2963.96, 2950.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19220) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 840.711 CPU sec, 52.951 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1230, 410932, 1046, 1318) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (465, 2012, 5426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15348) [M::mem_pestat] mean and std.dev: (3081.67, 2957.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20309) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (103, 152, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (172.43, 93.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 607) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (736, 2612, 6045) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16663) [M::mem_pestat] mean and std.dev: (3506.51, 3063.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21972) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (468, 1910, 5104) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14376) [M::mem_pestat] mean and std.dev: (3012.50, 2948.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19012) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 813.452 CPU sec, 51.262 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1245, 410668, 1029, 1257) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (547, 2108, 5515) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15451) [M::mem_pestat] mean and std.dev: (3173.46, 2971.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20419) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 152, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 479) [M::mem_pestat] mean and std.dev: (172.46, 93.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 604) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (874, 3046, 6145) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16687) [M::mem_pestat] mean and std.dev: (3677.47, 3018.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21958) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (421, 1874, 5369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15265) [M::mem_pestat] mean and std.dev: (3070.31, 3022.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20213) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 831.637 CPU sec, 52.511 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1273, 410979, 1049, 1299) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (445, 1933, 5221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14773) [M::mem_pestat] mean and std.dev: (3057.53, 2982.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19549) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (103, 151, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (172.38, 93.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 607) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (889, 3404, 6088) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16486) [M::mem_pestat] mean and std.dev: (3755.46, 3016.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21685) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (361, 1625, 4738) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13492) [M::mem_pestat] mean and std.dev: (2806.56, 2865.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17869) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 830.167 CPU sec, 52.311 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1235, 414658, 1054, 1199) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (423, 1836, 4974) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14076) [M::mem_pestat] mean and std.dev: (2885.41, 2852.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18627) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (103, 150, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469) [M::mem_pestat] mean and std.dev: (169.86, 91.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 591) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (847, 3016, 6080) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16546) [M::mem_pestat] mean and std.dev: (3660.33, 3031.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21779) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (450, 1927, 5144) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14532) [M::mem_pestat] mean and std.dev: (3053.94, 2964.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19226) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 839.080 CPU sec, 52.850 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1199, 416029, 990, 1243) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (463, 2023, 5500) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15574) [M::mem_pestat] mean and std.dev: (3139.11, 3000.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20611) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (102, 150, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (169.86, 91.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (793, 2940, 5822) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15880) [M::mem_pestat] mean and std.dev: (3556.18, 2973.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20909) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (481, 1974, 5191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14611) [M::mem_pestat] mean and std.dev: (2991.68, 2914.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19321) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 820.835 CPU sec, 51.729 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1219, 414997, 995, 1250) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (452, 2045, 5225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14771) [M::mem_pestat] mean and std.dev: (3088.81, 2933.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19544) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (103, 150, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (170.21, 91.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 595) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (889, 2958, 5805) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15637) [M::mem_pestat] mean and std.dev: (3540.70, 2949.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20553) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (494, 2015, 5169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14519) [M::mem_pestat] mean and std.dev: (3062.38, 2929.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19194) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 826.474 CPU sec, 52.137 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1234, 413556, 1018, 1196) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (493, 2071, 5444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15346) [M::mem_pestat] mean and std.dev: (3153.29, 2983.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20297) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (103, 150, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (171.00, 92.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 599) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (896, 2875, 5792) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15584) [M::mem_pestat] mean and std.dev: (3565.74, 2912.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20480) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (377, 1778, 4817) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13697) [M::mem_pestat] mean and std.dev: (2859.01, 2864.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18137) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 840.214 CPU sec, 52.983 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1205, 410205, 966, 1242) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (463, 1933, 5164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14566) [M::mem_pestat] mean and std.dev: (3045.58, 2938.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19267) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (103, 151, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (172.30, 94.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 607) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (915, 2723, 5877) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15801) [M::mem_pestat] mean and std.dev: (3514.11, 2909.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20763) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (487, 2027, 5302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14932) [M::mem_pestat] mean and std.dev: (3080.01, 2960.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19747) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 844.445 CPU sec, 53.194 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1230, 413982, 982, 1228) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (497, 2172, 5386) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15164) [M::mem_pestat] mean and std.dev: (3116.15, 2937.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20053) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (103, 151, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (171.00, 92.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 599) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (804, 2399, 5735) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15597) [M::mem_pestat] mean and std.dev: (3437.29, 2994.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20528) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (429, 2020, 5280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14982) [M::mem_pestat] mean and std.dev: (3102.79, 2968.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19833) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 818.703 CPU sec, 51.614 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1218, 414159, 1084, 1179) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (462, 1797, 5169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14583) [M::mem_pestat] mean and std.dev: (2994.55, 2916.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19290) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (103, 151, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (171.05, 92.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 599) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (759, 2816, 5820) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15942) [M::mem_pestat] mean and std.dev: (3516.45, 3015.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21003) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (433, 2010, 5284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14986) [M::mem_pestat] mean and std.dev: (3081.47, 2962.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19837) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 820.518 CPU sec, 51.785 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1231, 416423, 1064, 1293) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (460, 1933, 5341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15103) [M::mem_pestat] mean and std.dev: (3104.86, 2949.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19984) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (103, 150, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469) [M::mem_pestat] mean and std.dev: (169.79, 90.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 591) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (677, 2427, 5424) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14918) [M::mem_pestat] mean and std.dev: (3313.35, 2893.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19665) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (386, 1862, 5235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14933) [M::mem_pestat] mean and std.dev: (2958.06, 2913.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19782) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 838.543 CPU sec, 52.905 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1195, 416816, 1017, 1233) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (472, 2089, 5074) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14278) [M::mem_pestat] mean and std.dev: (3074.95, 2913.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18880) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (103, 150, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469) [M::mem_pestat] mean and std.dev: (169.53, 90.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 591) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (831, 2644, 5907) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16059) [M::mem_pestat] mean and std.dev: (3484.58, 2977.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21135) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (483, 2198, 5499) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15531) [M::mem_pestat] mean and std.dev: (3208.83, 2972.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20547) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 834.778 CPU sec, 52.660 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1234, 418976, 986, 1215) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (484, 1860, 4827) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13513) [M::mem_pestat] mean and std.dev: (2944.39, 2839.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17856) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (102, 149, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 468) [M::mem_pestat] mean and std.dev: (169.19, 90.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (725, 2526, 5860) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16130) [M::mem_pestat] mean and std.dev: (3376.05, 2992.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21265) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (483, 1902, 5175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14559) [M::mem_pestat] mean and std.dev: (3067.94, 2977.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19251) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 867.822 CPU sec, 54.704 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1315, 407545, 1022, 1321) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (501, 2068, 5105) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14313) [M::mem_pestat] mean and std.dev: (3039.85, 2830.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18917) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (103, 152, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (173.33, 95.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 611) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (750, 2638, 5443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14829) [M::mem_pestat] mean and std.dev: (3370.77, 2942.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19522) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (414, 1990, 5135) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14577) [M::mem_pestat] mean and std.dev: (3066.65, 2987.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19298) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 853.795 CPU sec, 53.841 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1211, 408753, 992, 1289) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (586, 2232, 5321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14791) [M::mem_pestat] mean and std.dev: (3166.88, 2900.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19526) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (103, 151, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (172.50, 94.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 607) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (737, 2685, 5981) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16469) [M::mem_pestat] mean and std.dev: (3515.80, 3007.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21713) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (417, 1841, 5248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14910) [M::mem_pestat] mean and std.dev: (3033.19, 3016.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19741) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 861.615 CPU sec, 54.270 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1189, 410058, 993, 1249) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (512, 2019, 5165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14471) [M::mem_pestat] mean and std.dev: (3139.36, 3001.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19124) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (103, 151, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (172.56, 94.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 607) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (808, 2813, 6009) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16411) [M::mem_pestat] mean and std.dev: (3560.30, 2997.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21612) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (396, 1561, 4759) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13485) [M::mem_pestat] mean and std.dev: (2867.77, 2911.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17848) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 859.368 CPU sec, 54.208 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1253, 407030, 1043, 1280) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (467, 1808, 5001) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14069) [M::mem_pestat] mean and std.dev: (2944.23, 2845.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18603) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (103, 152, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490) [M::mem_pestat] mean and std.dev: (174.26, 96.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 619) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (851, 2929, 5839) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15815) [M::mem_pestat] mean and std.dev: (3528.69, 2912.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20803) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (442, 2074, 5135) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14521) [M::mem_pestat] mean and std.dev: (3061.04, 2896.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19214) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 835.130 CPU sec, 52.628 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1231, 409068, 1099, 1204) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (529, 2231, 5269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14749) [M::mem_pestat] mean and std.dev: (3185.26, 2960.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19489) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (103, 151, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (172.98, 94.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 611) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (863, 2541, 5489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14741) [M::mem_pestat] mean and std.dev: (3367.83, 2849.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19367) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (376, 1667, 4729) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13435) [M::mem_pestat] mean and std.dev: (2793.64, 2845.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17788) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 816.073 CPU sec, 51.846 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1256, 407277, 1071, 1297) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (440, 2139, 5235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14825) [M::mem_pestat] mean and std.dev: (3094.64, 2963.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19620) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (103, 152, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (173.29, 95.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 611) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (820, 2647, 5707) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15481) [M::mem_pestat] mean and std.dev: (3414.94, 2894.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20368) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (402, 1820, 5025) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14271) [M::mem_pestat] mean and std.dev: (2970.11, 2951.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18894) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 806.505 CPU sec, 50.845 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1147, 413962, 925, 1220) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (446, 1922, 5207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14729) [M::mem_pestat] mean and std.dev: (3030.58, 2931.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19490) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (101, 147, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 464) [M::mem_pestat] mean and std.dev: (167.58, 90.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 585) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (600, 2381, 5228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14484) [M::mem_pestat] mean and std.dev: (3199.86, 2883.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19112) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (335, 1534, 4619) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13187) [M::mem_pestat] mean and std.dev: (2747.38, 2892.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17471) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 990.931 CPU sec, 62.408 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1165, 411554, 1089, 1277) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (484, 2082, 5082) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14278) [M::mem_pestat] mean and std.dev: (3092.65, 2915.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18876) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (103, 151, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (171.45, 93.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 603) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (780, 2390, 5641) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15363) [M::mem_pestat] mean and std.dev: (3366.48, 2965.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20224) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (440, 1878, 4958) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13994) [M::mem_pestat] mean and std.dev: (2950.06, 2885.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18512) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 805.590 CPU sec, 50.787 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1203, 411460, 972, 1243) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (392, 1838, 4955) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14081) [M::mem_pestat] mean and std.dev: (2981.00, 2972.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18644) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (103, 151, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (171.22, 92.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 599) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (808, 2620, 5810) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15814) [M::mem_pestat] mean and std.dev: (3470.14, 2980.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20816) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (403, 1698, 4993) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14173) [M::mem_pestat] mean and std.dev: (2925.24, 2950.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18763) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 811.565 CPU sec, 51.175 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1245, 410801, 1027, 1309) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (477, 1950, 5028) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14130) [M::mem_pestat] mean and std.dev: (3005.40, 2876.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18681) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (103, 151, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (171.47, 93.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 603) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (755, 2736, 5822) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15956) [M::mem_pestat] mean and std.dev: (3451.04, 2958.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21023) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (400, 1842, 5116) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14548) [M::mem_pestat] mean and std.dev: (2990.78, 2978.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19264) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 793.192 CPU sec, 50.031 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1203, 412104, 1067, 1182) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (564, 1963, 5104) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14184) [M::mem_pestat] mean and std.dev: (3051.91, 2856.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18724) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (103, 151, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (171.07, 92.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 599) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (829, 2392, 5574) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15064) [M::mem_pestat] mean and std.dev: (3290.39, 2859.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19809) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (434, 1859, 4819) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13589) [M::mem_pestat] mean and std.dev: (2931.90, 2905.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17974) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 815.648 CPU sec, 51.437 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1204, 412818, 1008, 1281) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (477, 2040, 5201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14649) [M::mem_pestat] mean and std.dev: (3056.05, 2899.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19373) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (103, 151, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (171.15, 92.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 599) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (716, 2735, 5652) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15524) [M::mem_pestat] mean and std.dev: (3436.10, 2933.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20460) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (363, 2041, 5204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14886) [M::mem_pestat] mean and std.dev: (2999.41, 2923.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19727) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 798.188 CPU sec, 50.356 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1110, 414058, 994, 1231) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (423, 1849, 4891) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13827) [M::mem_pestat] mean and std.dev: (2901.26, 2848.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18295) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (103, 150, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (170.44, 92.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 595) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (748, 2620, 5664) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15496) [M::mem_pestat] mean and std.dev: (3394.06, 2960.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20412) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (402, 1707, 4527) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12777) [M::mem_pestat] mean and std.dev: (2791.33, 2852.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16902) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 810.619 CPU sec, 51.145 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1237, 412588, 996, 1232) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (438, 2031, 5217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14775) [M::mem_pestat] mean and std.dev: (3040.62, 2956.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19554) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (103, 151, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (171.82, 93.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 607) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (752, 2833, 5660) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15476) [M::mem_pestat] mean and std.dev: (3456.03, 2923.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20384) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (468, 2000, 5137) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14475) [M::mem_pestat] mean and std.dev: (3008.75, 2894.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19144) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 810.665 CPU sec, 51.107 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1162, 412662, 928, 1288) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (390, 1745, 4909) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13947) [M::mem_pestat] mean and std.dev: (2874.95, 2892.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18466) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (102, 150, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (170.27, 92.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (643, 2601, 5896) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16402) [M::mem_pestat] mean and std.dev: (3417.90, 2969.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21655) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (441, 2413, 5233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14817) [M::mem_pestat] mean and std.dev: (3184.49, 2979.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19609) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 885.461 CPU sec, 55.855 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1293, 412489, 987, 1231) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (509, 2151, 5132) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14378) [M::mem_pestat] mean and std.dev: (3057.81, 2853.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19001) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (102, 150, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (170.18, 92.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (806, 2609, 5492) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14864) [M::mem_pestat] mean and std.dev: (3425.93, 2932.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19550) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (430, 1797, 5042) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14266) [M::mem_pestat] mean and std.dev: (2953.69, 2927.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18878) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 864.022 CPU sec, 54.484 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1297, 410887, 1008, 1256) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (450, 1867, 5108) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14424) [M::mem_pestat] mean and std.dev: (3030.99, 2955.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19082) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (103, 151, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (172.56, 94.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 607) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (709, 2821, 5846) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16120) [M::mem_pestat] mean and std.dev: (3526.40, 3026.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21257) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (398, 1670, 5193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14783) [M::mem_pestat] mean and std.dev: (2941.07, 2990.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19578) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 820.844 CPU sec, 51.757 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1186, 417862, 1015, 1194) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (411, 2116, 5327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15159) [M::mem_pestat] mean and std.dev: (3084.72, 2979.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20075) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (101, 147, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 458) [M::mem_pestat] mean and std.dev: (165.91, 88.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 577) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (779, 2546, 5775) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15767) [M::mem_pestat] mean and std.dev: (3434.06, 2979.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20763) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (363, 1989, 5060) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14454) [M::mem_pestat] mean and std.dev: (3017.79, 2937.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19151) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1019.924 CPU sec, 64.195 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1174, 419038, 967, 1119) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (514, 1985, 5594) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15754) [M::mem_pestat] mean and std.dev: (3203.00, 3064.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20834) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 144, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 450) [M::mem_pestat] mean and std.dev: (163.71, 87.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 567) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (710, 2618, 5791) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15953) [M::mem_pestat] mean and std.dev: (3505.84, 3014.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21034) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (357, 1743, 5145) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14721) [M::mem_pestat] mean and std.dev: (2923.67, 2937.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19509) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1070.058 CPU sec, 67.356 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1213, 412037, 930, 1287) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (456, 1901, 5076) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14316) [M::mem_pestat] mean and std.dev: (3014.21, 2959.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18936) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (101, 148, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470) [M::mem_pestat] mean and std.dev: (168.95, 91.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (896, 3028, 5937) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16019) [M::mem_pestat] mean and std.dev: (3611.07, 2948.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21060) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (429, 2027, 5161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14625) [M::mem_pestat] mean and std.dev: (3044.94, 2933.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19357) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1005.799 CPU sec, 63.404 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1202, 410519, 976, 1135) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (435, 1922, 5249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14877) [M::mem_pestat] mean and std.dev: (2989.25, 2911.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19691) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (102, 150, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (170.86, 93.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (622, 2370, 5326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14734) [M::mem_pestat] mean and std.dev: (3201.65, 2873.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19438) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (374, 1907, 5528) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15836) [M::mem_pestat] mean and std.dev: (3088.10, 3059.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20990) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 957.386 CPU sec, 60.274 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1208, 409348, 1014, 1188) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (517, 2148, 4969) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13873) [M::mem_pestat] mean and std.dev: (3066.81, 2854.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18325) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (102, 150, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480) [M::mem_pestat] mean and std.dev: (171.59, 94.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (827, 3004, 5959) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16223) [M::mem_pestat] mean and std.dev: (3603.06, 2994.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21355) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (482, 2047, 5452) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15392) [M::mem_pestat] mean and std.dev: (3146.89, 3004.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20362) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 934.816 CPU sec, 58.972 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1186, 407188, 986, 1209) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (436, 1944, 4916) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13876) [M::mem_pestat] mean and std.dev: (3020.40, 2909.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18356) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (103, 151, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (173.85, 96.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 615) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (786, 2556, 5628) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15312) [M::mem_pestat] mean and std.dev: (3398.08, 2950.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20154) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (379, 1789, 4998) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14236) [M::mem_pestat] mean and std.dev: (2922.78, 2895.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18855) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 881.885 CPU sec, 55.590 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1252, 409168, 1008, 1178) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (479, 1978, 5392) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15218) [M::mem_pestat] mean and std.dev: (3106.73, 2946.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20131) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (102, 150, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (171.11, 93.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (665, 2510, 5524) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15242) [M::mem_pestat] mean and std.dev: (3316.32, 2907.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20101) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (444, 2085, 5365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15207) [M::mem_pestat] mean and std.dev: (3078.32, 2906.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20128) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 957.223 CPU sec, 60.335 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1235, 404860, 1072, 1257) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (448, 1897, 4941) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13927) [M::mem_pestat] mean and std.dev: (2909.17, 2809.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18420) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (103, 152, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490) [M::mem_pestat] mean and std.dev: (174.70, 96.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 619) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (774, 2509, 5870) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16062) [M::mem_pestat] mean and std.dev: (3459.01, 3009.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21158) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (344, 1879, 5055) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14477) [M::mem_pestat] mean and std.dev: (2941.36, 2917.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19188) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 863.867 CPU sec, 54.459 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1247, 407382, 1105, 1204) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (460, 2055, 5566) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15778) [M::mem_pestat] mean and std.dev: (3167.24, 2994.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20884) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (103, 152, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (173.34, 95.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 611) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (758, 2440, 5652) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15440) [M::mem_pestat] mean and std.dev: (3377.77, 2916.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20334) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (495, 2135, 5264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14802) [M::mem_pestat] mean and std.dev: (3056.61, 2924.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19571) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 862.367 CPU sec, 54.381 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1218, 407274, 1120, 1264) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (469, 1988, 5335) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15067) [M::mem_pestat] mean and std.dev: (3061.63, 2962.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19933) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (103, 152, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (173.80, 95.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 615) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (673, 2469, 5566) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15352) [M::mem_pestat] mean and std.dev: (3328.93, 2943.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20245) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (378, 1717, 5103) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14553) [M::mem_pestat] mean and std.dev: (2947.13, 2956.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19278) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 856.345 CPU sec, 54.003 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1234, 409784, 1035, 1254) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (505, 2181, 5403) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15199) [M::mem_pestat] mean and std.dev: (3094.13, 2890.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20097) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (103, 151, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (172.28, 94.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 607) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (768, 2532, 5779) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15801) [M::mem_pestat] mean and std.dev: (3432.48, 3009.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20812) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (480, 2206, 5417) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15291) [M::mem_pestat] mean and std.dev: (3164.70, 3023.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20228) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 855.009 CPU sec, 53.990 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1196, 408126, 1035, 1240) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (494, 1852, 5156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14480) [M::mem_pestat] mean and std.dev: (3034.84, 2930.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19142) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (103, 151, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (172.96, 94.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 611) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (794, 2384, 5686) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15470) [M::mem_pestat] mean and std.dev: (3376.81, 2952.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20362) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (372, 1731, 4953) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14115) [M::mem_pestat] mean and std.dev: (2865.34, 2904.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18696) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 844.179 CPU sec, 53.218 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1240, 407810, 1027, 1294) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (453, 2045, 4988) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14058) [M::mem_pestat] mean and std.dev: (2984.52, 2853.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18593) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (103, 151, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (172.83, 94.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 611) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (825, 2870, 6007) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16371) [M::mem_pestat] mean and std.dev: (3557.39, 2992.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21553) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (384, 1898, 5233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14931) [M::mem_pestat] mean and std.dev: (3013.91, 2966.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19780) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 849.372 CPU sec, 53.545 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1169, 409203, 1025, 1201) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (444, 2036, 5184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14664) [M::mem_pestat] mean and std.dev: (3070.58, 2918.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19404) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (103, 151, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (172.99, 94.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 611) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (787, 2787, 5866) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16024) [M::mem_pestat] mean and std.dev: (3493.70, 2939.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21103) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (414, 2032, 5273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14991) [M::mem_pestat] mean and std.dev: (3051.42, 2926.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19850) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 855.529 CPU sec, 53.897 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1231, 410144, 1056, 1258) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (442, 1911, 5062) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14302) [M::mem_pestat] mean and std.dev: (2961.13, 2819.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18922) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (103, 151, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (172.26, 94.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 607) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (684, 2675, 6338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 17646) [M::mem_pestat] mean and std.dev: (3564.55, 3106.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 23300) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (372, 1770, 5378) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15390) [M::mem_pestat] mean and std.dev: (3024.80, 3033.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20396) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 862.935 CPU sec, 54.415 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1252, 408515, 1020, 1248) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (426, 2027, 5070) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14358) [M::mem_pestat] mean and std.dev: (3081.13, 3005.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19002) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (103, 151, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (172.88, 95.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 611) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (822, 2648, 5918) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16110) [M::mem_pestat] mean and std.dev: (3435.32, 2924.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21206) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (390, 1793, 4694) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13302) [M::mem_pestat] mean and std.dev: (2854.62, 2845.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17606) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 881.916 CPU sec, 55.563 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1260, 408367, 1041, 1320) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (561, 2325, 5682) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15924) [M::mem_pestat] mean and std.dev: (3297.16, 3034.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21045) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (103, 151, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (173.46, 95.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 615) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (738, 2461, 5424) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14796) [M::mem_pestat] mean and std.dev: (3253.51, 2848.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19482) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (434, 1942, 5465) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15527) [M::mem_pestat] mean and std.dev: (3100.98, 3007.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20558) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 831.542 CPU sec, 52.419 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1172, 411752, 1041, 1222) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (525, 2183, 5400) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15150) [M::mem_pestat] mean and std.dev: (3108.46, 2900.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20025) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (103, 150, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (171.15, 92.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 599) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (861, 2689, 5862) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15864) [M::mem_pestat] mean and std.dev: (3528.56, 2961.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20865) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (431, 1957, 5132) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14534) [M::mem_pestat] mean and std.dev: (3074.77, 2967.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19235) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 845.818 CPU sec, 53.305 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1163, 413585, 963, 1275) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (454, 1727, 4937) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13903) [M::mem_pestat] mean and std.dev: (2919.46, 2876.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18386) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (103, 150, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (170.27, 91.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 595) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1058, 2957, 5985) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15839) [M::mem_pestat] mean and std.dev: (3631.34, 2906.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20766) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (412, 1891, 5424) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15448) [M::mem_pestat] mean and std.dev: (3063.98, 3013.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20460) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 819.158 CPU sec, 51.816 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1269, 412638, 1026, 1197) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (466, 1935, 5080) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14308) [M::mem_pestat] mean and std.dev: (3031.80, 2917.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18922) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (103, 150, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (170.37, 92.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 595) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (677, 2376, 5553) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15305) [M::mem_pestat] mean and std.dev: (3306.91, 2922.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20181) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (425, 1928, 5205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14765) [M::mem_pestat] mean and std.dev: (3000.55, 2921.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19545) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 833.091 CPU sec, 52.509 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1168, 412619, 967, 1180) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (516, 2133, 5648) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15912) [M::mem_pestat] mean and std.dev: (3216.98, 3020.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21044) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (103, 150, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (170.90, 92.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 599) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (812, 2699, 5609) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15203) [M::mem_pestat] mean and std.dev: (3415.98, 2899.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20000) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (423, 2038, 4993) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14133) [M::mem_pestat] mean and std.dev: (2990.61, 2898.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18703) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 830.922 CPU sec, 52.531 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1157, 412211, 1036, 1277) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (446, 2071, 5422) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15374) [M::mem_pestat] mean and std.dev: (3073.56, 2885.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20350) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (103, 150, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (170.70, 92.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 599) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (880, 2609, 5661) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15223) [M::mem_pestat] mean and std.dev: (3439.64, 2936.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20004) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (460, 2069, 5216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14728) [M::mem_pestat] mean and std.dev: (3115.06, 2991.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19484) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 826.129 CPU sec, 52.160 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1135, 411567, 984, 1201) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (423, 1833, 5271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14967) [M::mem_pestat] mean and std.dev: (3034.47, 2980.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19815) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (103, 151, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (171.18, 92.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 599) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (779, 2613, 5956) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16310) [M::mem_pestat] mean and std.dev: (3514.31, 3039.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21487) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (446, 1933, 4794) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13490) [M::mem_pestat] mean and std.dev: (2921.38, 2818.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17838) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 838.345 CPU sec, 52.811 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1195, 409079, 1054, 1217) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (467, 2084, 5235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14771) [M::mem_pestat] mean and std.dev: (3085.05, 2931.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19539) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (103, 151, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (171.75, 93.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 603) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (873, 2699, 6170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16764) [M::mem_pestat] mean and std.dev: (3585.85, 3026.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 22061) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (470, 2194, 5248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14804) [M::mem_pestat] mean and std.dev: (3102.32, 2883.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19582) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 888.824 CPU sec, 55.988 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1219, 412197, 977, 1282) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (496, 1998, 5064) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14200) [M::mem_pestat] mean and std.dev: (3071.01, 2947.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18768) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (103, 151, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (172.18, 93.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 607) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (613, 2269, 5500) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15274) [M::mem_pestat] mean and std.dev: (3250.06, 2919.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20161) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (442, 1866, 5253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14875) [M::mem_pestat] mean and std.dev: (3028.58, 2952.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19686) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 834.453 CPU sec, 52.542 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1139, 414657, 963, 1225) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (467, 1936, 5088) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14330) [M::mem_pestat] mean and std.dev: (3024.80, 2928.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18951) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (102, 150, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (170.35, 92.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (687, 2688, 5770) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15936) [M::mem_pestat] mean and std.dev: (3383.04, 2930.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21019) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (440, 2003, 5084) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14372) [M::mem_pestat] mean and std.dev: (3016.38, 2901.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19016) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 845.741 CPU sec, 53.336 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1196, 412454, 1062, 1300) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (399, 1887, 4987) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14163) [M::mem_pestat] mean and std.dev: (2963.72, 2926.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18751) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (103, 150, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (170.89, 92.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 599) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (814, 2761, 5941) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16195) [M::mem_pestat] mean and std.dev: (3500.60, 2959.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21322) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (358, 1547, 4952) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14140) [M::mem_pestat] mean and std.dev: (2849.09, 2948.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18734) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 847.576 CPU sec, 53.374 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1180, 413254, 993, 1261) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (502, 2240, 5296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14884) [M::mem_pestat] mean and std.dev: (3186.50, 2970.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19678) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (102, 150, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (170.91, 92.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (735, 2586, 5576) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15258) [M::mem_pestat] mean and std.dev: (3378.58, 2936.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20099) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (417, 1973, 5217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14817) [M::mem_pestat] mean and std.dev: (3045.32, 2946.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19617) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 921.960 CPU sec, 58.070 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1027, 427836, 896, 1137) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (330, 1686, 4920) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14100) [M::mem_pestat] mean and std.dev: (2868.82, 2943.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18690) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (98, 142, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 431) [M::mem_pestat] mean and std.dev: (158.94, 82.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 542) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (646, 2492, 5637) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15619) [M::mem_pestat] mean and std.dev: (3334.93, 2962.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20610) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (421, 1968, 4788) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13522) [M::mem_pestat] mean and std.dev: (2932.88, 2824.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17889) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1117.025 CPU sec, 70.491 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1088, 417735, 957, 1165) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (495, 1956, 5190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14580) [M::mem_pestat] mean and std.dev: (3002.12, 2860.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19275) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (100, 146, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 454) [M::mem_pestat] mean and std.dev: (164.63, 87.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 572) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (796, 2546, 5769) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15715) [M::mem_pestat] mean and std.dev: (3451.46, 3007.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20688) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (416, 1828, 5106) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14486) [M::mem_pestat] mean and std.dev: (3004.84, 2948.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19176) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1054.092 CPU sec, 66.308 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1157, 415649, 944, 1220) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (432, 1910, 5022) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14202) [M::mem_pestat] mean and std.dev: (3001.77, 2919.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18792) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (101, 147, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461) [M::mem_pestat] mean and std.dev: (166.76, 89.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 581) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (829, 2719, 5699) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15439) [M::mem_pestat] mean and std.dev: (3503.71, 2994.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20309) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (363, 1566, 4763) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13563) [M::mem_pestat] mean and std.dev: (2805.58, 2936.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17963) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 974.038 CPU sec, 61.313 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1202, 411661, 1015, 1324) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (480, 1990, 5058) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14214) [M::mem_pestat] mean and std.dev: (3043.00, 2925.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18792) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (102, 150, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (170.19, 92.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (802, 2765, 6093) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16675) [M::mem_pestat] mean and std.dev: (3620.12, 3076.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21966) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (430, 1919, 5285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14995) [M::mem_pestat] mean and std.dev: (3039.98, 2952.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19850) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 930.691 CPU sec, 58.695 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1216, 409897, 978, 1171) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (450, 2045, 5465) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15495) [M::mem_pestat] mean and std.dev: (3120.48, 2988.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20510) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (102, 150, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (170.90, 93.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (757, 2524, 5974) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16408) [M::mem_pestat] mean and std.dev: (3554.13, 3065.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21625) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (391, 1839, 4831) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13711) [M::mem_pestat] mean and std.dev: (2902.24, 2896.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18151) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 911.034 CPU sec, 57.400 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1209, 413442, 1078, 1186) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (468, 2048, 5155) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14529) [M::mem_pestat] mean and std.dev: (3097.77, 2934.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19216) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (102, 149, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 468) [M::mem_pestat] mean and std.dev: (168.75, 90.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (765, 2615, 5677) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15501) [M::mem_pestat] mean and std.dev: (3394.31, 2880.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20413) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (419, 1955, 4972) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14078) [M::mem_pestat] mean and std.dev: (3009.96, 2943.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18631) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 903.880 CPU sec, 56.977 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1247, 411131, 1014, 1233) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (467, 2123, 5222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14732) [M::mem_pestat] mean and std.dev: (3081.68, 2919.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19487) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (102, 150, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (170.31, 92.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (668, 2444, 5396) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14852) [M::mem_pestat] mean and std.dev: (3284.45, 2934.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19580) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (459, 1918, 5111) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14415) [M::mem_pestat] mean and std.dev: (2984.20, 2872.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19067) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 899.291 CPU sec, 56.600 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1126, 411457, 1039, 1232) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (446, 1917, 5109) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14435) [M::mem_pestat] mean and std.dev: (2991.84, 2927.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19098) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (102, 150, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (170.85, 92.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (847, 2623, 5755) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15571) [M::mem_pestat] mean and std.dev: (3470.98, 2970.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20479) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (450, 1922, 5216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14748) [M::mem_pestat] mean and std.dev: (3020.55, 2905.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19514) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 871.399 CPU sec, 54.877 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1175, 413293, 945, 1221) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (517, 2328, 5551) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15619) [M::mem_pestat] mean and std.dev: (3251.60, 3026.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20653) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (103, 150, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (170.37, 91.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 595) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (811, 2525, 5584) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15130) [M::mem_pestat] mean and std.dev: (3410.16, 2928.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19903) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (407, 1889, 5049) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14333) [M::mem_pestat] mean and std.dev: (2944.73, 2868.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18975) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 870.145 CPU sec, 54.849 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1173, 411053, 966, 1242) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (443, 1865, 5065) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14309) [M::mem_pestat] mean and std.dev: (2966.52, 2892.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18931) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (103, 150, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (171.36, 93.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 603) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (726, 2655, 6035) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16653) [M::mem_pestat] mean and std.dev: (3508.45, 3016.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21962) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (442, 1924, 5107) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14437) [M::mem_pestat] mean and std.dev: (3014.52, 2932.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19102) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 856.041 CPU sec, 54.180 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1234, 413085, 1023, 1264) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (450, 2094, 5076) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14328) [M::mem_pestat] mean and std.dev: (3048.77, 2888.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18954) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (103, 150, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (170.97, 92.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 599) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (656, 2613, 5753) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15947) [M::mem_pestat] mean and std.dev: (3404.93, 2990.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21044) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (424, 1923, 5279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14989) [M::mem_pestat] mean and std.dev: (3077.77, 3038.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19844) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 857.007 CPU sec, 53.974 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1242, 412028, 1052, 1253) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (505, 2354, 5496) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15478) [M::mem_pestat] mean and std.dev: (3201.23, 2921.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20469) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (103, 151, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (171.01, 92.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 599) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (942, 2731, 5520) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14676) [M::mem_pestat] mean and std.dev: (3466.04, 2892.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19254) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (425, 1965, 5368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15254) [M::mem_pestat] mean and std.dev: (3110.55, 3015.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20197) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 852.657 CPU sec, 53.700 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1293, 405630, 1062, 1235) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (512, 2028, 5152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14432) [M::mem_pestat] mean and std.dev: (3067.08, 2870.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19072) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (103, 152, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (173.88, 95.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 615) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (679, 2538, 5555) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15307) [M::mem_pestat] mean and std.dev: (3366.64, 3009.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20183) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (376, 1797, 4990) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14218) [M::mem_pestat] mean and std.dev: (2931.49, 2905.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18832) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 856.559 CPU sec, 54.016 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1248, 410892, 1024, 1295) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (523, 1905, 5374) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15076) [M::mem_pestat] mean and std.dev: (3015.88, 2925.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19927) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (103, 151, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (172.31, 93.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 607) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (844, 2780, 5983) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16261) [M::mem_pestat] mean and std.dev: (3514.03, 2980.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21400) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (473, 2090, 5387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15215) [M::mem_pestat] mean and std.dev: (3135.34, 3007.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20129) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 846.306 CPU sec, 53.307 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1251, 411187, 1044, 1292) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (428, 1851, 4920) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13904) [M::mem_pestat] mean and std.dev: (2952.26, 2909.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18396) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (103, 151, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (171.75, 93.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 603) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (767, 2671, 6079) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16703) [M::mem_pestat] mean and std.dev: (3527.01, 3027.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 22015) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (507, 1945, 5048) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14130) [M::mem_pestat] mean and std.dev: (3009.00, 2877.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18671) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 805.257 CPU sec, 50.757 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1179, 411635, 1009, 1220) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (437, 1980, 5137) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14537) [M::mem_pestat] mean and std.dev: (3083.44, 2977.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19237) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (103, 151, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (172.41, 93.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 607) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (806, 2837, 5728) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15572) [M::mem_pestat] mean and std.dev: (3551.62, 3002.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20494) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (394, 1993, 5071) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14425) [M::mem_pestat] mean and std.dev: (3045.14, 2959.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19102) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 818.954 CPU sec, 51.621 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1182, 413903, 981, 1253) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (481, 1947, 5088) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14302) [M::mem_pestat] mean and std.dev: (3024.43, 2930.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18909) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (103, 151, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (171.12, 92.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 599) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (748, 2730, 5473) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14923) [M::mem_pestat] mean and std.dev: (3433.68, 2935.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19648) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (418, 1941, 5287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15025) [M::mem_pestat] mean and std.dev: (3008.35, 2934.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19894) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 819.592 CPU sec, 51.994 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1186, 414068, 1038, 1243) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (495, 2099, 5251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14763) [M::mem_pestat] mean and std.dev: (3082.65, 2905.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19519) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (103, 150, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (170.41, 91.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 595) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (896, 2853, 6186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16766) [M::mem_pestat] mean and std.dev: (3659.41, 3036.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 22056) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (420, 1848, 5153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14619) [M::mem_pestat] mean and std.dev: (2992.83, 2927.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19352) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 801.967 CPU sec, 50.632 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1219, 414035, 1003, 1172) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (444, 1951, 5353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15171) [M::mem_pestat] mean and std.dev: (3037.36, 2945.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20080) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (103, 150, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (170.22, 91.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 595) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (832, 2628, 5716) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15484) [M::mem_pestat] mean and std.dev: (3417.66, 2895.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20368) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (398, 2036, 5115) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14549) [M::mem_pestat] mean and std.dev: (3040.86, 2981.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19266) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 847.353 CPU sec, 53.428 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1203, 415153, 1033, 1254) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (551, 2262, 5598) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15692) [M::mem_pestat] mean and std.dev: (3240.54, 3014.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20739) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (103, 150, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (170.56, 91.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 595) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (675, 2505, 5085) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13905) [M::mem_pestat] mean and std.dev: (3270.03, 2862.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18315) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (400, 2159, 5435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15505) [M::mem_pestat] mean and std.dev: (3136.61, 3020.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20540) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 836.390 CPU sec, 52.692 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1205, 411086, 1052, 1232) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (419, 1963, 5159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14639) [M::mem_pestat] mean and std.dev: (3015.98, 2939.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19379) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (103, 151, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (172.05, 93.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 603) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (657, 2664, 5791) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16059) [M::mem_pestat] mean and std.dev: (3479.50, 3022.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21193) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (464, 1866, 5164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14564) [M::mem_pestat] mean and std.dev: (3025.45, 2944.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19264) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 845.606 CPU sec, 53.333 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1206, 412474, 946, 1234) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (523, 2057, 5118) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14308) [M::mem_pestat] mean and std.dev: (3088.17, 2916.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18903) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (103, 151, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (171.61, 93.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 603) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (797, 2722, 5883) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16055) [M::mem_pestat] mean and std.dev: (3506.49, 2956.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21141) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (435, 2089, 5205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14745) [M::mem_pestat] mean and std.dev: (3035.05, 2928.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19515) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 840.383 CPU sec, 53.014 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1214, 414241, 1092, 1200) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (505, 2043, 4946) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13828) [M::mem_pestat] mean and std.dev: (2953.82, 2806.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18269) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (103, 150, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (170.41, 91.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 595) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (661, 2563, 5385) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14833) [M::mem_pestat] mean and std.dev: (3270.84, 2879.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19557) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (392, 1939, 4823) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13685) [M::mem_pestat] mean and std.dev: (2935.70, 2895.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18116) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 842.305 CPU sec, 53.061 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1231, 414754, 1047, 1173) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (432, 1914, 4934) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13938) [M::mem_pestat] mean and std.dev: (2928.88, 2885.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18440) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (103, 151, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (171.11, 92.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 599) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (850, 2938, 5937) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16111) [M::mem_pestat] mean and std.dev: (3576.27, 2996.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21198) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (451, 2047, 5402) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15304) [M::mem_pestat] mean and std.dev: (3128.35, 2966.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20255) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 818.657 CPU sec, 51.585 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1143, 415966, 1006, 1216) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (526, 2072, 5552) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15604) [M::mem_pestat] mean and std.dev: (3238.86, 3061.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20630) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (103, 150, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469) [M::mem_pestat] mean and std.dev: (169.68, 91.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 591) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (837, 2674, 5619) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15183) [M::mem_pestat] mean and std.dev: (3479.80, 2954.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19965) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (423, 1900, 4945) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13989) [M::mem_pestat] mean and std.dev: (3007.09, 2914.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18511) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 846.872 CPU sec, 53.325 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1221, 418339, 1026, 1275) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (418, 1833, 5017) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14215) [M::mem_pestat] mean and std.dev: (2989.87, 2932.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18814) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (103, 150, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469) [M::mem_pestat] mean and std.dev: (169.70, 90.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 591) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (710, 2386, 5352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14636) [M::mem_pestat] mean and std.dev: (3259.64, 2917.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19278) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (480, 1908, 5124) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14412) [M::mem_pestat] mean and std.dev: (3067.18, 2986.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19056) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 859.125 CPU sec, 54.121 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1078, 426845, 882, 1130) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (383, 2045, 5013) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14273) [M::mem_pestat] mean and std.dev: (2986.84, 2896.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18903) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (98, 142, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 431) [M::mem_pestat] mean and std.dev: (159.15, 82.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 542) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (671, 2440, 5439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14975) [M::mem_pestat] mean and std.dev: (3331.06, 2943.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19743) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (373, 1786, 5201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14857) [M::mem_pestat] mean and std.dev: (2973.23, 2946.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19685) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1107.406 CPU sec, 69.707 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1133, 417359, 938, 1187) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (521, 1909, 5113) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14297) [M::mem_pestat] mean and std.dev: (3036.64, 2909.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18889) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (100, 146, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 454) [M::mem_pestat] mean and std.dev: (164.46, 87.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 572) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (683, 2491, 5791) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16007) [M::mem_pestat] mean and std.dev: (3372.67, 2968.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21115) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (318, 1674, 4523) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12933) [M::mem_pestat] mean and std.dev: (2779.43, 2830.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17138) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 1030.365 CPU sec, 64.821 real sec [M::process] read 1080450 sequences (162067500 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1155, 416229, 980, 1181) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (428, 1767, 4997) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14135) [M::mem_pestat] mean and std.dev: (2915.77, 2908.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18704) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (101, 147, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 458) [M::mem_pestat] mean and std.dev: (165.94, 88.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 577) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (811, 2699, 6159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16855) [M::mem_pestat] mean and std.dev: (3568.82, 3041.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 22203) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (381, 1721, 4912) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13974) [M::mem_pestat] mean and std.dev: (2849.24, 2889.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18505) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 952.124 CPU sec, 59.870 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1006, 328496, 854, 1022) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (641, 2356, 5382) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14864) [M::mem_pestat] mean and std.dev: (3249.51, 2899.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19605) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (103, 152, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490) [M::mem_pestat] mean and std.dev: (174.35, 96.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 619) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (867, 2512, 5537) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14877) [M::mem_pestat] mean and std.dev: (3376.86, 2892.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19547) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (459, 1686, 5193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14661) [M::mem_pestat] mean and std.dev: (2991.70, 2958.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19395) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1080450 reads in 697.275 CPU sec, 43.866 real sec [main] Version: 0.7.17-r1188 [main] CMD: bwa mem -SP5M -t 20 /oak/stanford/groups/akundaje/marinovg/ENCODE4/datasets-gDNA/L2786-bdellovibrio_bacteriovorus-ICPB_3264_gDNA_cloudy/SPAdes-3.15.4-2023-04-19-B_b_gDNA_check.pass-V2/bwa-indexes/scaffolds.fa /oak/stanford/groups/akundaje/marinovg/ENCODE4/DNAzoo/2023-09-26-Novogene-NovaSeq/juicer-L2869+L2870+L2873+L2874_HEPES/splits/L2869+L2870+L2873+L2874_R1.fastq.gz /oak/stanford/groups/akundaje/marinovg/ENCODE4/DNAzoo/2023-09-26-Novogene-NovaSeq/juicer-L2869+L2870+L2873+L2874_HEPES/splits/L2869+L2870+L2873+L2874_R2.fastq.gz [main] Real time: 23701.563 sec; CPU: 375569.079 sec Picked up _JAVA_OPTIONS: -Xmx16384m Iterated 1000 times, returning estimate Picked up _JAVA_OPTIONS: -Xmx16384m Picked up _JAVA_OPTIONS: -Xmx16384m Picked up _JAVA_OPTIONS: -Xmx16384m Could not read hic file: Index: 0, Size: 0