#Chromosome	Start	End	Name	GUIDE-Seq Reads	Strand	Cells	Targetsite	Target_Sequence	Offtarget_Sequence	20 bp protospacer # mismatches	3 bp PAM # mismatches	Mismatch Total
chr6	43737456	43737476	tru_VEGFA_site3_chr6_43737470_3140	3140	+	U2OS	tru_VEGFA_site3	GAGTGAGTGTGTGCGTGNGG	GAGTGAGTGTGTGCGTGTGG	0	0	0
chr14	65569152	65569172	tru_VEGFA_site3_chr14_65569159_2698	2698	-	U2OS	tru_VEGFA_site3	GAGTGAGTGTGTGCGTGNGG	GAGTGAGTGTGTGTGTGGGG	1	0	1
chr22	37662817	37662837	tru_VEGFA_site3_chr22_37662824_1334	1334	-	U2OS	tru_VEGFA_site3	GAGTGAGTGTGTGCGTGNGG	GAGTGAGTGTATGCGTGTGG	1	0	1
chr19	40561860	40561880	tru_VEGFA_site3_chr19_40561867_859	859	-	U2OS	tru_VEGFA_site3	GAGTGAGTGTGTGCGTGNGG	GTGTGAGTGTGTGCGTGAGG	1	0	1
chr5	89440962	89440982	tru_VEGFA_site3_chr5_89440969_826	826	-	U2OS	tru_VEGFA_site3	GAGTGAGTGTGTGCGTGNGG	GAGTGAGTGTGTGCATGAGG	1	0	1
chr19	47732485	47732505	tru_VEGFA_site3_chr19_47732493_406	406	-	U2OS	tru_VEGFA_site3	GAGTGAGTGTGTGCGTGNGG	GAGTGAGTGTGTGTGTGTGG	1	0	1
chr10	98760581	98760601	tru_VEGFA_site3_chr10_98760588_319	319	-	U2OS	tru_VEGFA_site3	GAGTGAGTGTGTGCGTGNGG	GAGTGAATGTGTGCGTGAGG	1	0	1
chr5	115434661	115434681	tru_VEGFA_site3_chr5_115434677_215	215	+	U2OS	tru_VEGFA_site3	GAGTGAGTGTGTGCGTGNGG	GGGTGAGTGTGTGCGTGAGG	1	0	1
chr10	105307458	105307478	tru_VEGFA_site3_chr10_105307472_183	183	+	U2OS	tru_VEGFA_site3	GAGTGAGTGTGTGCGTGNGG	GTGTGAGTGTGTGCGTGGGG	1	0	1
chr8	48997791	48997811	tru_VEGFA_site3_chr8_48997805_105	105	+	U2OS	tru_VEGFA_site3	GAGTGAGTGTGTGCGTGNGG	GAGTGAGTGTGTGTGTGTGG	1	0	1
chr14	44199328	44199348	tru_VEGFA_site3_chr14_44199342_75	75	+	U2OS	tru_VEGFA_site3	GAGTGAGTGTGTGCGTGNGG	GAGTGAGTGTGTGTGTGGTG	1	1	2
chr16	80016316	80016336	tru_VEGFA_site3_chr16_80016330_50	50	+	U2OS	tru_VEGFA_site3	GAGTGAGTGTGTGCGTGNGG	GAGTGAGTGTGTGCGTGTGA	0	1	1
chr3	71632802	71632822	tru_VEGFA_site3_chr3_71632809_30	30	-	U2OS	tru_VEGFA_site3	GAGTGAGTGTGTGCGTGNGG	GAGTGAGTGTGTGCGCGGGG	1	0	1
chr12	57612402	57612422	tru_VEGFA_site3_chr12_57612416_7	7	+	U2OS	tru_VEGFA_site3	GAGTGAGTGTGTGCGTGNGG	GAGTGAGAGTGAGCGTGAGG	2	0	2
chr5	177239504	177239524	tru_VEGFA_site3_chr5_177239512_2	2	-	U2OS	tru_VEGFA_site3	GAGTGAGTGTGTGCGTGNGG	GGGTGAGGGTGTGCGGGTGA	3	1	4
chr6	44149310	44149330	tru_VEGFA_site3_chr6_44149312_2	2	-	U2OS	tru_VEGFA_site3	GAGTGAGTGTGTGCGTGNGG	ATGTGAGTGTGTATGTGTGG	4	0	4