#Chromosome	Start	End	Name	GUIDE-Seq Reads	Strand	Cells	Targetsite	Target_Sequence	Offtarget_Sequence	20 bp protospacer # mismatches	3 bp PAM # mismatches	Mismatch Total
chr17	39796321	39796342	tru_VEGFA_site1_chr17_39796328_4434	4434	-	U2OS	tru_VEGFA_site1	GTGGGGGGAGTTTGCTCCNGG	GTGGAGGGAGCTTGCTCCTGG	2	0	2
chr6	43737290	43737311	tru_VEGFA_site1_chr6_43737297_3729	3729	-	U2OS	tru_VEGFA_site1	GTGGGGGGAGTTTGCTCCNGG	GTGGGGGGAGTTTGCTCCTGG	0	0	0
chr15	65637530	65637551	tru_VEGFA_site1_chr15_65637537_1947	1947	-	U2OS	tru_VEGFA_site1	GTGGGGGGAGTTTGCTCCNGG	ATGGAGGGAGTTTGCTCCTGG	2	0	2
chr22	37215260	37215281	tru_VEGFA_site1_chr22_37215275_995	995	+	U2OS	tru_VEGFA_site1	GTGGGGGGAGTTTGCTCCNGG	GTGGGGGGAGTTTGCCCCAGG	1	0	1
chr5	32945259	32945280	tru_VEGFA_site1_chr5_32945274_672	672	+	U2OS	tru_VEGFA_site1	GTGGGGGGAGTTTGCTCCNGG	GTGGGGGGTGTTTGCTCCCGG	1	0	1
chr12	1988061	1988082	tru_VEGFA_site1_chr12_1988076_224	224	+	U2OS	tru_VEGFA_site1	GTGGGGGGAGTTTGCTCCNGG	GGGGAGGGAGTTTGCTCCTGG	2	0	2
chr5	139263008	139263029	tru_VEGFA_site1_chr5_139263023_183	183	+	U2OS	tru_VEGFA_site1	GTGGGGGGAGTTTGCTCCNGG	GGGGGGGCAGTTTGCTCCTGG	2	0	2
chr6	50485666	50485687	tru_VEGFA_site1_chr6_50485681_69	69	+	U2OS	tru_VEGFA_site1	GTGGGGGGAGTTTGCTCCNGG	GTGTGGGGAATTTGCTCCAGG	2	0	2
chr4	8453787	8453808	tru_VEGFA_site1_chr4_8453802_23	23	+	U2OS	tru_VEGFA_site1	GTGGGGGGAGTTTGCTCCNGG	GTGGGTGGAGTTTGCTACAGG	2	0	2
chr17	47317532	47317553	tru_VEGFA_site1_chr17_47317539_6	6	-	U2OS	tru_VEGFA_site1	GTGGGGGGAGTTTGCTCCNGG	GGTGGGGGAGCTTGCTCCAGG	3	0	3