#Chromosome	Start	End	Name	GUIDE-Seq Reads	Strand	Cells	Targetsite	Target_Sequence	Offtarget_Sequence	20 bp protospacer # mismatches	3 bp PAM # mismatches	Mismatch Total
chr15	65637530	65637553	VEGFA_site1_chr15_65637537_3237	3237	-	U2OS	VEGFA_site1	GGGTGGGGGGAGTTTGCTCCNGG	GGATGGAGGGAGTTTGCTCCTGG	2	0	2
chr6	43737290	43737313	VEGFA_site1_chr6_43737297_2685	2685	-	U2OS	VEGFA_site1	GGGTGGGGGGAGTTTGCTCCNGG	GGGTGGGGGGAGTTTGCTCCTGG	0	0	0
chr17	39796321	39796344	VEGFA_site1_chr17_39796328_1935	1935	-	U2OS	VEGFA_site1	GGGTGGGGGGAGTTTGCTCCNGG	TAGTGGAGGGAGCTTGCTCCTGG	4	0	4
chr1	99347644	99347667	VEGFA_site1_chr1_99347651_819	819	-	U2OS	VEGFA_site1	GGGTGGGGGGAGTTTGCTCCNGG	GGGGAGGGGAAGTTTGCTCCTGG	3	0	3
chr12	131690181	131690204	VEGFA_site1_chr12_131690198_597	597	+	U2OS	VEGFA_site1	GGGTGGGGGGAGTTTGCTCCNGG	GGGAGGGTGGAGTTTGCTCCTGG	2	0	2
chr12	1988059	1988082	VEGFA_site1_chr12_1988076_375	375	+	U2OS	VEGFA_site1	GGGTGGGGGGAGTTTGCTCCNGG	CGGGGGAGGGAGTTTGCTCCTGG	3	0	3
chr17	47317532	47317555	VEGFA_site1_chr17_47317539_324	324	-	U2OS	VEGFA_site1	GGGTGGGGGGAGTTTGCTCCNGG	CTGGTGGGGGAGCTTGCTCCAGG	5	0	5
chr22	37215258	37215281	VEGFA_site1_chr22_37215275_249	249	+	U2OS	VEGFA_site1	GGGTGGGGGGAGTTTGCTCCNGG	GGGTGGGGGGAGTTTGCCCCAGG	1	0	1
chr5	32945257	32945280	VEGFA_site1_chr5_32945275_246	246	+	U2OS	VEGFA_site1	GGGTGGGGGGAGTTTGCTCCNGG	GCGTGGGGGGTGTTTGCTCCCGG	2	0	2
chr6	14316366	14316389	VEGFA_site1_chr6_14316373_241	241	-	U2OS	VEGFA_site1	GGGTGGGGGGAGTTTGCTCCNGG	GTGGGGGTAGAGTTTGCTCCAGG	4	0	4
chr5	7067141	7067164	VEGFA_site1_chr5_7067158_115	115	+	U2OS	VEGFA_site1	GGGTGGGGGGAGTTTGCTCCNGG	GAGGGTGGGGAGTTTACTCCTGG	4	0	4
chr1	233157336	233157359	VEGFA_site1_chr1_233157353_58	58	+	U2OS	VEGFA_site1	GGGTGGGGGGAGTTTGCTCCNGG	GGAGGAGGGGAGTCTGCTCCAGG	4	0	4
chr5	56172072	56172095	VEGFA_site1_chr5_56172097_50	50	-	U2OS	VEGFA_site1	GGGTGGGGGGAGTTTGCTCCNGG	GGTGGGGGTGGGTTTGCTCCTGG	4	0	4
chr22	19698456	19698479	VEGFA_site1_chr22_19698463_47	47	-	U2OS	VEGFA_site1	GGGTGGGGGGAGTTTGCTCCNGG	GAGGGGGAGCAGTTTGCTCCAGG	4	0	4
chr4	8453785	8453808	VEGFA_site1_chr4_8453802_24	24	+	U2OS	VEGFA_site1	GGGTGGGGGGAGTTTGCTCCNGG	GAGTGGGTGGAGTTTGCTACAGG	3	0	3
chr3	128284314	128284337	VEGFA_site1_chr3_128284321_16	16	-	U2OS	VEGFA_site1	GGGTGGGGGGAGTTTGCTCCNGG	AGGTGGTGGGAGCTTGTTCCTGG	4	0	4
chr11	117481201	117481224	VEGFA_site1_chr11_117481208_14	14	-	U2OS	VEGFA_site1	GGGTGGGGGGAGTTTGCTCCNGG	GGGCAAGGGGAGGTTGCTCCTGG	4	0	4
chr3	195871257	195871280	VEGFA_site1_chr3_195871264_11	11	-	U2OS	VEGFA_site1	GGGTGGGGGGAGTTTGCTCCNGG	GGTGGGGGAGAGCTAGCTCCGGG	5	0	5
chr11	67574244	67574267	VEGFA_site1_chr11_67574261_7	7	+	U2OS	VEGFA_site1	GGGTGGGGGGAGTTTGCTCCNGG	AGGAAGGAGGAGTTAGCTCCTGG	5	0	5
chr13	26202794	26202817	VEGFA_site1_chr13_26202811_6	6	+	U2OS	VEGFA_site1	GGGTGGGGGGAGTTTGCTCCNGG	GGTTGAGGGGAGTCTGCTCCAGG	3	0	3
chr16	8763206	8763229	VEGFA_site1_chr16_8763213_5	5	-	U2OS	VEGFA_site1	GGGTGGGGGGAGTTTGCTCCNGG	AAGTAAGGGAAGTTTGCTCCTGG	5	0	5
chr3	125633985	125634008	VEGFA_site1_chr3_125633992_4	4	-	U2OS	VEGFA_site1	GGGTGGGGGGAGTTTGCTCCNGG	AGGAAGGAGGAGTTAGCTCCTGG	5	0	5