#Chromosome	Start	End	Name	GUIDE-Seq Reads	Strand	Cells	Targetsite	Target_Sequence	Offtarget_Sequence	20 bp protospacer # mismatches	3 bp PAM # mismatches	Mismatch Total
chr2	73160981	73161004	EMX1_chr2_73160998_4521	4521	+	U2OS	EMX1	GAGTCCGAGCAGAAGAAGAANGG	GAGTCCGAGCAGAAGAAGAAGGG	0	0	0
chr5	45359060	45359083	EMX1_chr5_45359067_3123	3123	-	U2OS	EMX1	GAGTCCGAGCAGAAGAAGAANGG	GAGTTAGAGCAGAAGAAGAAAGG	2	0	2
chr15	44109746	44109769	EMX1_chr15_44109763_1445	1445	+	U2OS	EMX1	GAGTCCGAGCAGAAGAAGAANGG	GAGTCTAAGCAGAAGAAGAAGAG	2	1	3
chr2	219845055	219845078	EMX1_chr2_219845072_700	700	+	U2OS	EMX1	GAGTCCGAGCAGAAGAAGAANGG	GAGGCCGAGCAGAAGAAAGACGG	3	0	3
chr8	128801241	128801264	EMX1_chr8_128801258_390	390	+	U2OS	EMX1	GAGTCCGAGCAGAAGAAGAANGG	GAGTCCTAGCAGGAGAAGAAGAG	2	1	3
chr5	9227145	9227168	EMX1_chr5_9227162_258	258	+	U2OS	EMX1	GAGTCCGAGCAGAAGAAGAANGG	AAGTCTGAGCACAAGAAGAATGG	3	0	3
chrX	53467704	53467727	EMX1_chrX_53467711_216	216	-	U2OS	EMX1	GAGTCCGAGCAGAAGAAGAANGG	GAGTCCGGGAAGGAGAAGAAAGG	3	0	3
chr5	146833183	146833206	EMX1_chr5_146833190_143	143	-	U2OS	EMX1	GAGTCCGAGCAGAAGAAGAANGG	GAGCCGGAGCAGAAGAAGGAGGG	3	0	3
chr1	23720611	23720634	EMX1_chr1_23720617_102	102	-	U2OS	EMX1	GAGTCCGAGCAGAAGAAGAANGG	AAGTCCGAGGAGAGGAAGAAAGG	3	0	3
chr3	5031597	5031620	EMX1_chr3_5031614_67	67	+	U2OS	EMX1	GAGTCCGAGCAGAAGAAGAANGG	GAATCCAAGCAGGAGAAGAAGGA	3	1	4
chr6	9118792	9118815	EMX1_chr6_9118799_38	38	-	U2OS	EMX1	GAGTCCGAGCAGAAGAAGAANGG	ACGTCTGAGCAGAAGAAGAATGG	3	0	3
chr13	27769640	27769663	EMX1_chr13_27769657_7	7	+	U2OS	EMX1	GAGTCCGAGCAGAAGAAGAANGG	GAGTAGGAGCAGGAGAAGAAGGA	3	1	4
chr15	100292461	100292484	EMX1_chr15_100292479_6	6	+	U2OS	EMX1	GAGTCCGAGCAGAAGAAGAANGG	AAGTCCCGGCAGAGGAAGAAGGG	4	0	4
chr3	95690179	95690202	EMX1_chr3_95690186_5	5	-	U2OS	EMX1	GAGTCCGAGCAGAAGAAGAANGG	TCATCCAAGCAGAAGAAGAAGAG	4	1	5
chr2	218378101	218378124	EMX1_chr2_218378108_2	2	-	U2OS	EMX1	GAGTCCGAGCAGAAGAAGAANGG	GAGTCTAAGCAGGAGAATAAAGG	4	0	4
chr10	58848711	58848734	EMX1_chr10_58848728_2	2	+	U2OS	EMX1	GAGTCCGAGCAGAAGAAGAANGG	GAGCACGAGCAAGAGAAGAAGGG	4	0	4