[M::bwa_idx_load_from_disk] read 0 ALT contigs [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7434, 405007, 6833, 7308) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1225, 3249, 6144) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15982) [M::mem_pestat] mean and std.dev: (3817.29, 2900.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20901) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (177, 212, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (35, 390) [M::mem_pestat] mean and std.dev: (210.97, 51.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 461) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1411, 3461, 6301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16081) [M::mem_pestat] mean and std.dev: (4010.79, 2855.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20971) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1439, 3464, 6276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15950) [M::mem_pestat] mean and std.dev: (3976.36, 2848.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20787) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (200000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 403.140 CPU sec, 55.142 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7650, 406353, 6641, 7223) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1242, 3183, 5996) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15504) [M::mem_pestat] mean and std.dev: (3757.12, 2868.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20258) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (177, 212, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (35, 390) [M::mem_pestat] mean and std.dev: (210.73, 51.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 461) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1466, 3529, 6244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15800) [M::mem_pestat] mean and std.dev: (4015.03, 2832.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20578) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1355, 3326, 6225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15965) [M::mem_pestat] mean and std.dev: (3908.30, 2861.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20835) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 360.458 CPU sec, 26.106 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7353, 405160, 6761, 7322) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1234, 3193, 6109) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15859) [M::mem_pestat] mean and std.dev: (3822.84, 2904.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20734) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (177, 212, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (35, 390) [M::mem_pestat] mean and std.dev: (210.88, 51.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 461) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1471, 3548, 6317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16009) [M::mem_pestat] mean and std.dev: (4034.27, 2843.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20855) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1334, 3297, 6216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15980) [M::mem_pestat] mean and std.dev: (3907.28, 2869.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20862) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (200000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 358.998 CPU sec, 24.849 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7529, 405904, 6901, 7140) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1252, 3243, 6151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15949) [M::mem_pestat] mean and std.dev: (3838.53, 2917.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20848) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (177, 212, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (35, 390) [M::mem_pestat] mean and std.dev: (210.79, 51.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 461) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1471, 3471, 6241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15781) [M::mem_pestat] mean and std.dev: (4001.36, 2823.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20551) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1391, 3394, 6360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16298) [M::mem_pestat] mean and std.dev: (3980.39, 2878.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21267) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (200000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 358.718 CPU sec, 24.191 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7496, 406341, 6944, 7231) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1309, 3299, 6145) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15817) [M::mem_pestat] mean and std.dev: (3868.21, 2884.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20653) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (177, 212, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (35, 390) [M::mem_pestat] mean and std.dev: (210.76, 51.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 461) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1458, 3484, 6268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15888) [M::mem_pestat] mean and std.dev: (3994.15, 2818.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20698) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1393, 3403, 6167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15715) [M::mem_pestat] mean and std.dev: (3932.24, 2836.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20489) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (200000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 362.413 CPU sec, 24.402 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7348, 406737, 6890, 7274) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1302, 3367, 6281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16239) [M::mem_pestat] mean and std.dev: (3896.83, 2890.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21218) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (177, 212, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (35, 390) [M::mem_pestat] mean and std.dev: (210.62, 51.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 461) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1470, 3455, 6222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15726) [M::mem_pestat] mean and std.dev: (4003.40, 2839.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20478) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1357, 3330, 6143) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15715) [M::mem_pestat] mean and std.dev: (3893.00, 2839.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20501) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (200000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 359.563 CPU sec, 23.499 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7436, 406936, 6884, 7187) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1230, 3255, 6125) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15915) [M::mem_pestat] mean and std.dev: (3824.51, 2894.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20810) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (177, 212, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (35, 390) [M::mem_pestat] mean and std.dev: (210.48, 51.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 461) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1476, 3529, 6315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15993) [M::mem_pestat] mean and std.dev: (4034.67, 2831.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20832) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1352, 3330, 6188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15860) [M::mem_pestat] mean and std.dev: (3916.50, 2868.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20696) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 361.090 CPU sec, 23.351 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7422, 406595, 7071, 7192) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1229, 3225, 6083) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15791) [M::mem_pestat] mean and std.dev: (3807.54, 2874.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20645) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (177, 212, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (35, 390) [M::mem_pestat] mean and std.dev: (210.52, 51.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 461) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1397, 3404, 6141) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15629) [M::mem_pestat] mean and std.dev: (3932.43, 2819.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20373) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1391, 3406, 6269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16025) [M::mem_pestat] mean and std.dev: (3952.93, 2873.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20903) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 358.984 CPU sec, 23.483 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7490, 406784, 6941, 7108) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1240, 3197, 6128) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15904) [M::mem_pestat] mean and std.dev: (3815.96, 2898.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20792) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (177, 211, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (35, 390) [M::mem_pestat] mean and std.dev: (210.40, 51.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 461) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1380, 3388, 6182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15786) [M::mem_pestat] mean and std.dev: (3932.64, 2851.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20588) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1375, 3388, 6165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15745) [M::mem_pestat] mean and std.dev: (3910.70, 2826.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20535) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (200000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 359.262 CPU sec, 23.293 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7437, 406963, 6955, 7342) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1228, 3183, 6147) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15985) [M::mem_pestat] mean and std.dev: (3807.99, 2897.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20904) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 211, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (32, 392) [M::mem_pestat] mean and std.dev: (210.37, 51.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 464) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1457, 3523, 6354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16148) [M::mem_pestat] mean and std.dev: (4029.88, 2848.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21045) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1393, 3351, 6155) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15679) [M::mem_pestat] mean and std.dev: (3903.70, 2832.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20441) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (200000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 355.327 CPU sec, 22.768 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7426, 407869, 6923, 7293) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1257, 3210, 6053) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15645) [M::mem_pestat] mean and std.dev: (3799.27, 2873.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20441) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 211, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (34, 389) [M::mem_pestat] mean and std.dev: (210.26, 51.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 460) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1397, 3424, 6197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15797) [M::mem_pestat] mean and std.dev: (3952.08, 2839.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20597) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1343, 3300, 6147) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15755) [M::mem_pestat] mean and std.dev: (3889.83, 2842.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20559) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 355.484 CPU sec, 23.374 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7447, 408226, 6839, 7128) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1231, 3205, 6194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16120) [M::mem_pestat] mean and std.dev: (3837.01, 2920.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21083) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 211, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (34, 389) [M::mem_pestat] mean and std.dev: (209.91, 51.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 460) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1472, 3514, 6373) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16175) [M::mem_pestat] mean and std.dev: (4038.55, 2855.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21076) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1369, 3458, 6272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16078) [M::mem_pestat] mean and std.dev: (3940.28, 2844.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20981) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (200000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 354.557 CPU sec, 22.829 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7285, 408487, 6968, 7249) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1265, 3319, 6115) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15815) [M::mem_pestat] mean and std.dev: (3840.28, 2877.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20665) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 211, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (34, 389) [M::mem_pestat] mean and std.dev: (209.94, 51.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 460) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1489, 3480, 6320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15982) [M::mem_pestat] mean and std.dev: (4028.97, 2842.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20813) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1416, 3425, 6355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16233) [M::mem_pestat] mean and std.dev: (3978.30, 2869.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21172) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 356.067 CPU sec, 23.033 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7435, 405766, 6790, 7348) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1228, 3124, 6095) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15829) [M::mem_pestat] mean and std.dev: (3765.06, 2884.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20696) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (177, 212, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (35, 390) [M::mem_pestat] mean and std.dev: (210.73, 51.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 461) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1458, 3411, 6203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15693) [M::mem_pestat] mean and std.dev: (3969.31, 2812.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20438) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1463, 3450, 6268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15878) [M::mem_pestat] mean and std.dev: (3983.95, 2844.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20683) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 359.860 CPU sec, 23.464 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7532, 405343, 6796, 7186) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1249, 3257, 6164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15994) [M::mem_pestat] mean and std.dev: (3831.76, 2894.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20909) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (177, 212, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (35, 390) [M::mem_pestat] mean and std.dev: (210.93, 51.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 461) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1446, 3479, 6225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15783) [M::mem_pestat] mean and std.dev: (3998.44, 2823.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20562) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1453, 3486, 6219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15751) [M::mem_pestat] mean and std.dev: (3972.24, 2834.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20517) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 361.380 CPU sec, 23.551 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7373, 405952, 7000, 7181) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1209, 3197, 6048) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15726) [M::mem_pestat] mean and std.dev: (3769.72, 2874.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20565) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (177, 212, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (35, 390) [M::mem_pestat] mean and std.dev: (210.99, 51.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 461) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1484, 3560, 6388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16196) [M::mem_pestat] mean and std.dev: (4071.63, 2843.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21100) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1422, 3396, 6246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15894) [M::mem_pestat] mean and std.dev: (3945.72, 2839.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20718) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 361.465 CPU sec, 23.213 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7360, 405582, 6812, 7161) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1245, 3321, 6229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16197) [M::mem_pestat] mean and std.dev: (3867.48, 2909.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21181) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (177, 212, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (35, 390) [M::mem_pestat] mean and std.dev: (210.82, 51.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 461) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1504, 3522, 6357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16063) [M::mem_pestat] mean and std.dev: (4054.27, 2847.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20916) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1396, 3438, 6197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15799) [M::mem_pestat] mean and std.dev: (3941.30, 2835.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20600) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (200000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 362.574 CPU sec, 23.185 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7546, 405373, 6916, 7240) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1258, 3224, 6173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16003) [M::mem_pestat] mean and std.dev: (3836.88, 2877.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20918) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (177, 212, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (35, 390) [M::mem_pestat] mean and std.dev: (210.95, 51.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 461) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1487, 3500, 6225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15701) [M::mem_pestat] mean and std.dev: (4018.08, 2831.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20439) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1372, 3415, 6246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15994) [M::mem_pestat] mean and std.dev: (3932.99, 2850.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20868) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (200000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 358.900 CPU sec, 23.212 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7461, 405310, 6871, 7203) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1230, 3251, 6206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16158) [M::mem_pestat] mean and std.dev: (3827.51, 2889.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21134) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (177, 212, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (35, 390) [M::mem_pestat] mean and std.dev: (210.66, 51.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 461) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1435, 3518, 6299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16027) [M::mem_pestat] mean and std.dev: (4030.93, 2866.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20891) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1341, 3348, 6102) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15624) [M::mem_pestat] mean and std.dev: (3890.04, 2833.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20385) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (200000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 359.280 CPU sec, 22.983 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7423, 405904, 6868, 7230) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1261, 3182, 6097) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15769) [M::mem_pestat] mean and std.dev: (3787.90, 2861.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20605) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (177, 212, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (35, 390) [M::mem_pestat] mean and std.dev: (210.77, 51.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 461) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1459, 3514, 6180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15622) [M::mem_pestat] mean and std.dev: (4000.01, 2813.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20343) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1417, 3363, 6271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15979) [M::mem_pestat] mean and std.dev: (3958.81, 2861.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20833) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (200000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 363.267 CPU sec, 23.378 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7430, 406446, 6954, 7110) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1237, 3195, 6140) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15946) [M::mem_pestat] mean and std.dev: (3802.25, 2874.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20849) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (177, 212, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (35, 390) [M::mem_pestat] mean and std.dev: (210.52, 51.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 461) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1429, 3437, 6234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15844) [M::mem_pestat] mean and std.dev: (3973.42, 2831.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20649) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1433, 3477, 6215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15779) [M::mem_pestat] mean and std.dev: (3993.90, 2863.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20561) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (200000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 362.708 CPU sec, 23.217 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7441, 405717, 6854, 7185) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1281, 3240, 6176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15966) [M::mem_pestat] mean and std.dev: (3830.72, 2886.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20861) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (177, 212, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (35, 390) [M::mem_pestat] mean and std.dev: (210.54, 51.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 461) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1518, 3524, 6264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15756) [M::mem_pestat] mean and std.dev: (4040.95, 2829.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20502) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1398, 3356, 6191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15777) [M::mem_pestat] mean and std.dev: (3919.28, 2844.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20570) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 363.335 CPU sec, 23.256 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7367, 407053, 6950, 7278) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1207, 3204, 5987) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15547) [M::mem_pestat] mean and std.dev: (3764.51, 2864.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20327) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 211, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (32, 392) [M::mem_pestat] mean and std.dev: (210.30, 51.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 464) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1461, 3449, 6248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15822) [M::mem_pestat] mean and std.dev: (4002.54, 2827.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20609) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1382, 3377, 6312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16172) [M::mem_pestat] mean and std.dev: (3946.09, 2849.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21102) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 358.908 CPU sec, 23.275 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7293, 406513, 6717, 7119) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1235, 3223, 6090) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15800) [M::mem_pestat] mean and std.dev: (3797.28, 2873.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20655) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 211, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (32, 392) [M::mem_pestat] mean and std.dev: (210.30, 51.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 464) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1504, 3468, 6240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15712) [M::mem_pestat] mean and std.dev: (4000.03, 2825.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20448) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1386, 3329, 6183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15777) [M::mem_pestat] mean and std.dev: (3909.57, 2844.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20574) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (200000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 358.899 CPU sec, 22.969 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7283, 406297, 6919, 7157) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1218, 3165, 6119) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15921) [M::mem_pestat] mean and std.dev: (3794.12, 2893.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20822) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 211, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (34, 389) [M::mem_pestat] mean and std.dev: (210.16, 51.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 460) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1484, 3505, 6255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15797) [M::mem_pestat] mean and std.dev: (4010.64, 2815.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20568) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1402, 3415, 6381) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16339) [M::mem_pestat] mean and std.dev: (3987.99, 2888.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21318) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (200000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 362.870 CPU sec, 23.237 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7445, 406468, 6832, 7151) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1253, 3200, 6072) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15710) [M::mem_pestat] mean and std.dev: (3805.82, 2865.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20529) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 211, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (34, 389) [M::mem_pestat] mean and std.dev: (210.15, 51.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 460) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1452, 3548, 6275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15921) [M::mem_pestat] mean and std.dev: (4015.01, 2836.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20744) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1352, 3315, 6236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16004) [M::mem_pestat] mean and std.dev: (3906.13, 2853.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20888) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (200000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 364.048 CPU sec, 23.387 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7354, 405888, 6968, 7185) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1222, 3250, 6218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16210) [M::mem_pestat] mean and std.dev: (3843.47, 2916.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21206) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (177, 212, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (35, 390) [M::mem_pestat] mean and std.dev: (210.56, 51.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 461) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1478, 3483, 6184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15596) [M::mem_pestat] mean and std.dev: (3994.08, 2809.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20302) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1354, 3350, 6194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15874) [M::mem_pestat] mean and std.dev: (3920.43, 2861.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20714) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (200000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 359.776 CPU sec, 23.109 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7396, 404883, 6853, 7223) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1250, 3240, 6053) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15659) [M::mem_pestat] mean and std.dev: (3792.55, 2869.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20462) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (177, 212, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (35, 390) [M::mem_pestat] mean and std.dev: (210.86, 51.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 461) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1466, 3374, 6227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15749) [M::mem_pestat] mean and std.dev: (3976.24, 2824.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20510) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1394, 3346, 6238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15926) [M::mem_pestat] mean and std.dev: (3945.67, 2845.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20770) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 360.366 CPU sec, 23.123 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7370, 406103, 6859, 7408) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1253, 3257, 6242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16220) [M::mem_pestat] mean and std.dev: (3862.88, 2914.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21209) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (177, 212, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (35, 390) [M::mem_pestat] mean and std.dev: (210.92, 51.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 461) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1493, 3450, 6146) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15452) [M::mem_pestat] mean and std.dev: (3954.14, 2784.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20105) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1398, 3442, 6258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15978) [M::mem_pestat] mean and std.dev: (3970.07, 2866.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20838) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (200000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 362.241 CPU sec, 23.239 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7374, 406096, 6736, 7333) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1253, 3275, 6208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16118) [M::mem_pestat] mean and std.dev: (3844.76, 2893.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21073) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (177, 212, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (35, 390) [M::mem_pestat] mean and std.dev: (210.85, 51.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 461) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1472, 3480, 6276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15884) [M::mem_pestat] mean and std.dev: (4007.58, 2828.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20688) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1392, 3335, 6156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15684) [M::mem_pestat] mean and std.dev: (3895.79, 2838.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20448) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (200000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 365.476 CPU sec, 23.467 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7412, 405938, 6778, 7086) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1205, 3214, 6067) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15791) [M::mem_pestat] mean and std.dev: (3784.98, 2894.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20653) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (177, 212, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (35, 390) [M::mem_pestat] mean and std.dev: (210.88, 51.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 461) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1450, 3471, 6359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16177) [M::mem_pestat] mean and std.dev: (4018.18, 2860.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21086) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1389, 3403, 6308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16146) [M::mem_pestat] mean and std.dev: (3975.63, 2889.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21065) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 359.717 CPU sec, 23.097 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7414, 405954, 6661, 7354) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1194, 3194, 6202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16218) [M::mem_pestat] mean and std.dev: (3806.21, 2909.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21226) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (177, 212, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (35, 390) [M::mem_pestat] mean and std.dev: (210.66, 51.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 461) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1447, 3467, 6191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15679) [M::mem_pestat] mean and std.dev: (3968.88, 2812.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20423) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1374, 3339, 6295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16137) [M::mem_pestat] mean and std.dev: (3939.01, 2872.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21058) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 361.053 CPU sec, 23.048 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7452, 406502, 6817, 7241) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1298, 3238, 6115) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15749) [M::mem_pestat] mean and std.dev: (3849.11, 2876.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20566) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (177, 212, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (35, 390) [M::mem_pestat] mean and std.dev: (210.77, 51.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 461) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1473, 3523, 6339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16071) [M::mem_pestat] mean and std.dev: (4038.09, 2873.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20937) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1418, 3362, 6293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16043) [M::mem_pestat] mean and std.dev: (3959.98, 2869.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20918) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 360.739 CPU sec, 23.221 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7457, 407142, 6936, 7132) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1230, 3158, 6193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16119) [M::mem_pestat] mean and std.dev: (3797.98, 2894.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21082) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (177, 212, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (35, 390) [M::mem_pestat] mean and std.dev: (210.63, 51.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 461) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1477, 3591, 6320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16006) [M::mem_pestat] mean and std.dev: (4048.52, 2840.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20849) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1320, 3280, 6200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15960) [M::mem_pestat] mean and std.dev: (3885.65, 2855.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20840) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 364.186 CPU sec, 23.200 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7317, 406826, 7021, 7198) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1237, 3246, 6099) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15823) [M::mem_pestat] mean and std.dev: (3799.70, 2883.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20685) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 211, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (32, 392) [M::mem_pestat] mean and std.dev: (210.33, 51.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 464) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1498, 3511, 6305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15919) [M::mem_pestat] mean and std.dev: (4032.05, 2836.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20726) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1402, 3376, 6214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15838) [M::mem_pestat] mean and std.dev: (3926.58, 2831.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20650) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (200000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 364.988 CPU sec, 23.480 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7612, 405794, 6884, 7258) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1269, 3275, 6189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16029) [M::mem_pestat] mean and std.dev: (3849.98, 2875.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20949) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 211, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (32, 392) [M::mem_pestat] mean and std.dev: (210.28, 51.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 464) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1466, 3441, 6281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15911) [M::mem_pestat] mean and std.dev: (3998.40, 2828.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20726) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1359, 3405, 6361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16365) [M::mem_pestat] mean and std.dev: (3965.41, 2896.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21367) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (200000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 360.959 CPU sec, 23.167 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7267, 406442, 6969, 7153) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1214, 3182, 6143) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16001) [M::mem_pestat] mean and std.dev: (3793.45, 2893.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20930) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 211, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (34, 389) [M::mem_pestat] mean and std.dev: (210.24, 51.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 460) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1454, 3484, 6233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15791) [M::mem_pestat] mean and std.dev: (4005.29, 2814.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20570) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1382, 3336, 6206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15854) [M::mem_pestat] mean and std.dev: (3926.25, 2865.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20678) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (200000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 360.284 CPU sec, 23.039 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7339, 406685, 6856, 7210) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1247, 3245, 6110) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15836) [M::mem_pestat] mean and std.dev: (3821.19, 2884.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20699) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 211, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (34, 389) [M::mem_pestat] mean and std.dev: (210.17, 51.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 460) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1514, 3504, 6368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16076) [M::mem_pestat] mean and std.dev: (4053.20, 2847.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20930) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1358, 3356, 6178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15818) [M::mem_pestat] mean and std.dev: (3894.82, 2832.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20638) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 361.578 CPU sec, 23.256 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7439, 406929, 6867, 7360) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1317, 3347, 6249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16113) [M::mem_pestat] mean and std.dev: (3893.64, 2876.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21045) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 211, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (34, 389) [M::mem_pestat] mean and std.dev: (210.02, 51.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 460) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1422, 3512, 6294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16038) [M::mem_pestat] mean and std.dev: (3997.48, 2841.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20910) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1343, 3312, 6145) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15749) [M::mem_pestat] mean and std.dev: (3880.79, 2843.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20551) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (200000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 361.149 CPU sec, 23.146 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7444, 406143, 6844, 7331) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1213, 3199, 6037) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15685) [M::mem_pestat] mean and std.dev: (3789.50, 2876.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20509) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 211, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (32, 392) [M::mem_pestat] mean and std.dev: (210.33, 51.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 464) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1487, 3518, 6312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15962) [M::mem_pestat] mean and std.dev: (4039.58, 2850.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20787) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1339, 3309, 6199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15919) [M::mem_pestat] mean and std.dev: (3873.90, 2831.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20779) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (200000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 365.197 CPU sec, 23.400 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7641, 404809, 6772, 7117) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1225, 3218, 6133) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15949) [M::mem_pestat] mean and std.dev: (3808.06, 2899.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20857) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (177, 212, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (35, 390) [M::mem_pestat] mean and std.dev: (210.88, 51.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 461) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1488, 3510, 6314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15966) [M::mem_pestat] mean and std.dev: (4013.82, 2827.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20792) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1395, 3374, 6174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15732) [M::mem_pestat] mean and std.dev: (3902.33, 2820.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20511) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 366.087 CPU sec, 23.652 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7468, 404926, 6824, 7135) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1231, 3184, 6102) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15844) [M::mem_pestat] mean and std.dev: (3766.63, 2859.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20715) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (177, 212, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (35, 390) [M::mem_pestat] mean and std.dev: (210.93, 51.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 461) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1449, 3460, 6253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15861) [M::mem_pestat] mean and std.dev: (3999.00, 2844.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20665) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1438, 3444, 6305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16039) [M::mem_pestat] mean and std.dev: (3971.90, 2842.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20906) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 362.321 CPU sec, 23.197 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7535, 404485, 6921, 7277) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1269, 3252, 6095) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15747) [M::mem_pestat] mean and std.dev: (3826.63, 2875.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20573) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (177, 212, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (35, 390) [M::mem_pestat] mean and std.dev: (210.92, 51.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 461) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1491, 3615, 6387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16179) [M::mem_pestat] mean and std.dev: (4069.66, 2836.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21075) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1373, 3261, 6182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15800) [M::mem_pestat] mean and std.dev: (3894.89, 2859.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20609) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 361.817 CPU sec, 23.534 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7525, 406027, 6847, 7269) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1234, 3255, 6134) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15934) [M::mem_pestat] mean and std.dev: (3829.20, 2886.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20834) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] (25, 50, 75) percentile: (177, 212, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (35, 390) [M::mem_pestat] mean and std.dev: (210.73, 51.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 461) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1484, 3522, 6367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16133) [M::mem_pestat] mean and std.dev: (4064.83, 2853.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21016) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1341, 3328, 6229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16005) [M::mem_pestat] mean and std.dev: (3909.64, 2858.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20893) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 362.678 CPU sec, 23.176 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7479, 406098, 7037, 7246) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1225, 3249, 6069) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15757) [M::mem_pestat] mean and std.dev: (3816.91, 2874.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20601) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (177, 212, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (35, 390) [M::mem_pestat] mean and std.dev: (210.77, 51.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 461) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1398, 3364, 6167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15705) [M::mem_pestat] mean and std.dev: (3934.45, 2837.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20474) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1363, 3349, 6168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15778) [M::mem_pestat] mean and std.dev: (3914.28, 2858.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20583) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 359.957 CPU sec, 23.184 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7521, 405903, 6762, 7178) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1257, 3236, 6105) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15801) [M::mem_pestat] mean and std.dev: (3824.07, 2890.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20649) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (177, 212, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (35, 390) [M::mem_pestat] mean and std.dev: (210.60, 51.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 461) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1462, 3445, 6235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15781) [M::mem_pestat] mean and std.dev: (3987.48, 2832.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20554) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1381, 3427, 6227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15919) [M::mem_pestat] mean and std.dev: (3937.15, 2826.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20765) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (200000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 357.361 CPU sec, 22.917 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7410, 406582, 6986, 7222) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1244, 3240, 6133) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15911) [M::mem_pestat] mean and std.dev: (3815.96, 2870.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20800) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (177, 212, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (35, 390) [M::mem_pestat] mean and std.dev: (210.68, 51.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 461) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1502, 3520, 6219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15653) [M::mem_pestat] mean and std.dev: (4012.92, 2811.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20370) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1387, 3348, 6241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15949) [M::mem_pestat] mean and std.dev: (3926.44, 2854.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20803) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (200000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 357.664 CPU sec, 22.886 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7365, 405995, 6566, 7396) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1284, 3296, 6193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16011) [M::mem_pestat] mean and std.dev: (3871.32, 2903.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20920) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (177, 212, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (35, 390) [M::mem_pestat] mean and std.dev: (210.62, 51.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 461) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1464, 3553, 6294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15954) [M::mem_pestat] mean and std.dev: (4039.61, 2840.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20784) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1394, 3372, 6241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15935) [M::mem_pestat] mean and std.dev: (3924.97, 2847.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20782) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (200000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 360.565 CPU sec, 23.161 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7319, 406180, 6863, 7145) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1221, 3303, 6146) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15996) [M::mem_pestat] mean and std.dev: (3844.39, 2893.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20921) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (177, 211, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (35, 390) [M::mem_pestat] mean and std.dev: (210.47, 51.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 461) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1389, 3416, 6331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16215) [M::mem_pestat] mean and std.dev: (3979.31, 2864.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21157) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1416, 3430, 6221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15831) [M::mem_pestat] mean and std.dev: (3954.50, 2841.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20636) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (200000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 358.351 CPU sec, 22.953 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7247, 407506, 6870, 7065) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1226, 3199, 6260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16328) [M::mem_pestat] mean and std.dev: (3845.48, 2923.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21362) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 211, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (32, 392) [M::mem_pestat] mean and std.dev: (210.43, 51.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 464) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1423, 3521, 6220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15814) [M::mem_pestat] mean and std.dev: (4003.54, 2834.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20611) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1324, 3345, 6179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15889) [M::mem_pestat] mean and std.dev: (3893.04, 2843.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20744) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (200000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 363.346 CPU sec, 23.355 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7342, 407413, 6823, 7148) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1238, 3151, 6112) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15860) [M::mem_pestat] mean and std.dev: (3789.14, 2870.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20734) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 211, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (32, 392) [M::mem_pestat] mean and std.dev: (210.28, 51.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 464) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1467, 3474, 6227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15747) [M::mem_pestat] mean and std.dev: (3982.06, 2820.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20507) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1348, 3389, 6171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15817) [M::mem_pestat] mean and std.dev: (3909.50, 2841.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20640) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 362.889 CPU sec, 23.469 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7264, 408141, 6778, 7226) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1246, 3175, 6037) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15619) [M::mem_pestat] mean and std.dev: (3761.21, 2850.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20410) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 211, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (34, 389) [M::mem_pestat] mean and std.dev: (210.14, 51.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 460) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1432, 3521, 6247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15877) [M::mem_pestat] mean and std.dev: (4007.65, 2853.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20692) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1350, 3384, 6162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15786) [M::mem_pestat] mean and std.dev: (3909.00, 2834.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20598) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 356.420 CPU sec, 22.762 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7489, 405323, 6901, 7207) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1187, 3178, 6071) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15839) [M::mem_pestat] mean and std.dev: (3774.09, 2898.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20723) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (177, 212, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (35, 390) [M::mem_pestat] mean and std.dev: (210.81, 51.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 461) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1435, 3479, 6264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15922) [M::mem_pestat] mean and std.dev: (3985.14, 2832.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20751) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1364, 3361, 6268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16076) [M::mem_pestat] mean and std.dev: (3938.78, 2861.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20980) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 367.319 CPU sec, 23.884 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7401, 404233, 6895, 7225) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1238, 3241, 6133) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15923) [M::mem_pestat] mean and std.dev: (3813.17, 2880.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20818) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (177, 212, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (35, 390) [M::mem_pestat] mean and std.dev: (210.88, 51.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 461) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1511, 3532, 6258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15752) [M::mem_pestat] mean and std.dev: (4014.43, 2819.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20499) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1368, 3316, 6192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15840) [M::mem_pestat] mean and std.dev: (3915.15, 2866.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20664) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 372.304 CPU sec, 23.967 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7481, 405244, 6915, 7086) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1217, 3180, 6071) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15779) [M::mem_pestat] mean and std.dev: (3763.94, 2883.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20633) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (177, 212, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (35, 390) [M::mem_pestat] mean and std.dev: (210.87, 51.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 461) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1445, 3523, 6403) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16319) [M::mem_pestat] mean and std.dev: (4044.05, 2863.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21277) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1390, 3411, 6294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16102) [M::mem_pestat] mean and std.dev: (3967.86, 2885.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21006) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 369.181 CPU sec, 23.611 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7375, 405341, 6863, 7294) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1224, 3259, 6164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16044) [M::mem_pestat] mean and std.dev: (3823.06, 2882.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20984) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (177, 212, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (35, 390) [M::mem_pestat] mean and std.dev: (210.90, 51.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 461) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1442, 3433, 6172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15632) [M::mem_pestat] mean and std.dev: (3962.94, 2807.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20362) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1389, 3429, 6286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16080) [M::mem_pestat] mean and std.dev: (3974.02, 2874.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20977) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 367.200 CPU sec, 23.748 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7362, 405932, 6849, 7242) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1218, 3183, 6140) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15984) [M::mem_pestat] mean and std.dev: (3788.39, 2890.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20906) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (177, 212, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (35, 390) [M::mem_pestat] mean and std.dev: (210.98, 51.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 461) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1524, 3547, 6251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15705) [M::mem_pestat] mean and std.dev: (4048.08, 2820.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20432) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1376, 3307, 6155) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15713) [M::mem_pestat] mean and std.dev: (3911.24, 2844.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20492) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (200000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 363.875 CPU sec, 23.214 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7467, 406264, 6878, 7162) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1253, 3237, 6171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16007) [M::mem_pestat] mean and std.dev: (3832.95, 2886.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20925) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (177, 212, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (35, 390) [M::mem_pestat] mean and std.dev: (210.66, 51.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 461) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1483, 3541, 6287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15895) [M::mem_pestat] mean and std.dev: (4029.81, 2818.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20699) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1417, 3465, 6292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16042) [M::mem_pestat] mean and std.dev: (3962.61, 2846.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20917) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 361.672 CPU sec, 23.197 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7352, 406392, 6860, 7320) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1267, 3281, 6138) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15880) [M::mem_pestat] mean and std.dev: (3836.42, 2880.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20751) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (177, 212, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (35, 390) [M::mem_pestat] mean and std.dev: (210.61, 51.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 461) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1467, 3524, 6256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15834) [M::mem_pestat] mean and std.dev: (4011.98, 2823.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20623) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1443, 3367, 6262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15900) [M::mem_pestat] mean and std.dev: (3947.79, 2837.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20719) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 363.317 CPU sec, 23.609 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7248, 405315, 6856, 7297) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1283, 3295, 6253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16193) [M::mem_pestat] mean and std.dev: (3891.61, 2907.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21163) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 212, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (32, 392) [M::mem_pestat] mean and std.dev: (210.58, 51.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 464) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1426, 3469, 6319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16105) [M::mem_pestat] mean and std.dev: (3999.98, 2847.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20998) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1425, 3412, 6327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16131) [M::mem_pestat] mean and std.dev: (3987.86, 2880.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21033) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (200000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 363.547 CPU sec, 23.157 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7489, 406337, 6792, 7190) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1219, 3161, 6076) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15790) [M::mem_pestat] mean and std.dev: (3765.44, 2872.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20647) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (177, 212, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (35, 390) [M::mem_pestat] mean and std.dev: (210.49, 51.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 461) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1448, 3497, 6329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16091) [M::mem_pestat] mean and std.dev: (4011.82, 2839.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20972) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1430, 3464, 6298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16034) [M::mem_pestat] mean and std.dev: (3966.58, 2840.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20902) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (200000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 361.995 CPU sec, 23.163 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7468, 406872, 6883, 7103) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1218, 3126, 6090) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15834) [M::mem_pestat] mean and std.dev: (3776.74, 2889.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20706) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 212, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (32, 392) [M::mem_pestat] mean and std.dev: (210.44, 51.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 464) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1494, 3453, 6231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15705) [M::mem_pestat] mean and std.dev: (4002.52, 2826.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20442) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1383, 3334, 6181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15777) [M::mem_pestat] mean and std.dev: (3909.86, 2836.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20575) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 363.779 CPU sec, 23.327 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7343, 406395, 6897, 7258) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1209, 3205, 6115) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15927) [M::mem_pestat] mean and std.dev: (3798.24, 2885.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20833) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 211, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (32, 392) [M::mem_pestat] mean and std.dev: (210.39, 51.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 464) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1488, 3514, 6243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15753) [M::mem_pestat] mean and std.dev: (4017.32, 2827.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20508) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1399, 3342, 6132) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15598) [M::mem_pestat] mean and std.dev: (3899.16, 2829.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20331) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 364.503 CPU sec, 23.555 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7581, 407324, 6772, 7134) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1259, 3299, 6102) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15788) [M::mem_pestat] mean and std.dev: (3825.69, 2873.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20631) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 211, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (34, 389) [M::mem_pestat] mean and std.dev: (210.18, 51.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 460) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1453, 3510, 6183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15643) [M::mem_pestat] mean and std.dev: (3969.15, 2816.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20373) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1357, 3417, 6219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15943) [M::mem_pestat] mean and std.dev: (3935.68, 2853.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20805) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 373.397 CPU sec, 23.872 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7307, 404723, 6898, 7313) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1277, 3145, 6096) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15734) [M::mem_pestat] mean and std.dev: (3808.73, 2860.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20553) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 211, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (34, 389) [M::mem_pestat] mean and std.dev: (210.27, 51.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 460) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1404, 3472, 6194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15774) [M::mem_pestat] mean and std.dev: (3964.39, 2828.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20564) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1370, 3335, 6197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15851) [M::mem_pestat] mean and std.dev: (3899.44, 2842.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20678) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (200000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 372.027 CPU sec, 23.773 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7344, 406009, 6831, 7253) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1214, 3278, 6143) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16001) [M::mem_pestat] mean and std.dev: (3826.79, 2898.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20930) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (177, 212, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (35, 390) [M::mem_pestat] mean and std.dev: (210.78, 51.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 461) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1464, 3452, 6265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15867) [M::mem_pestat] mean and std.dev: (4007.21, 2823.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20668) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1389, 3398, 6214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15864) [M::mem_pestat] mean and std.dev: (3939.84, 2849.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20689) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (200000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 371.066 CPU sec, 23.847 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7498, 404845, 6816, 7171) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1209, 3184, 6044) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15714) [M::mem_pestat] mean and std.dev: (3768.45, 2877.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20549) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (177, 212, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (35, 390) [M::mem_pestat] mean and std.dev: (210.87, 51.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 461) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1473, 3558, 6356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16122) [M::mem_pestat] mean and std.dev: (4036.49, 2844.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21005) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1363, 3475, 6242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16000) [M::mem_pestat] mean and std.dev: (3958.21, 2858.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20879) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 367.367 CPU sec, 23.822 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7369, 405308, 6983, 7354) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1218, 3200, 6100) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15864) [M::mem_pestat] mean and std.dev: (3784.57, 2899.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20746) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (177, 212, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (35, 390) [M::mem_pestat] mean and std.dev: (210.98, 51.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 461) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1434, 3421, 6311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16065) [M::mem_pestat] mean and std.dev: (3993.76, 2859.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20942) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1332, 3317, 6240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16056) [M::mem_pestat] mean and std.dev: (3900.42, 2865.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20964) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 365.349 CPU sec, 23.353 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7472, 405467, 6823, 7154) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1244, 3225, 6030) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15602) [M::mem_pestat] mean and std.dev: (3781.17, 2851.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20388) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (177, 212, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (35, 390) [M::mem_pestat] mean and std.dev: (210.88, 51.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 461) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1456, 3532, 6258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15862) [M::mem_pestat] mean and std.dev: (4013.05, 2826.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20664) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1386, 3379, 6244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15960) [M::mem_pestat] mean and std.dev: (3933.83, 2851.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20818) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (200000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 363.227 CPU sec, 23.216 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7623, 405888, 6915, 7204) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1174, 3069, 6047) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15793) [M::mem_pestat] mean and std.dev: (3730.19, 2885.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20666) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (177, 212, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (35, 390) [M::mem_pestat] mean and std.dev: (210.91, 51.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 461) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1457, 3404, 6188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15650) [M::mem_pestat] mean and std.dev: (3972.22, 2835.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20381) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1435, 3495, 6304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16042) [M::mem_pestat] mean and std.dev: (4000.00, 2855.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20911) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 364.884 CPU sec, 23.421 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7344, 405244, 6900, 7115) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1254, 3318, 6245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16227) [M::mem_pestat] mean and std.dev: (3872.68, 2898.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21218) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (177, 212, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (35, 390) [M::mem_pestat] mean and std.dev: (210.71, 51.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 461) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1450, 3492, 6256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15868) [M::mem_pestat] mean and std.dev: (3997.02, 2833.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20674) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1364, 3370, 6297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16163) [M::mem_pestat] mean and std.dev: (3945.17, 2870.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21096) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (200000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 363.645 CPU sec, 23.410 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7453, 405801, 6894, 7329) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1208, 3191, 6191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16157) [M::mem_pestat] mean and std.dev: (3802.02, 2904.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21140) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (177, 212, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (35, 390) [M::mem_pestat] mean and std.dev: (210.69, 51.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 461) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1477, 3441, 6252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15802) [M::mem_pestat] mean and std.dev: (4015.59, 2848.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20577) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1325, 3354, 6212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15986) [M::mem_pestat] mean and std.dev: (3912.65, 2859.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20873) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 362.660 CPU sec, 23.411 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7340, 405896, 6874, 7258) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1253, 3230, 6107) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15815) [M::mem_pestat] mean and std.dev: (3825.94, 2884.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20669) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (177, 212, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (35, 390) [M::mem_pestat] mean and std.dev: (210.57, 51.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 461) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1443, 3455, 6304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16026) [M::mem_pestat] mean and std.dev: (4003.85, 2846.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20887) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1378, 3344, 6298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16138) [M::mem_pestat] mean and std.dev: (3932.91, 2874.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21058) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 363.631 CPU sec, 23.519 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7477, 406573, 6830, 7059) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1278, 3229, 6156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15912) [M::mem_pestat] mean and std.dev: (3831.88, 2865.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20790) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 211, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (32, 392) [M::mem_pestat] mean and std.dev: (210.42, 51.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 464) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1468, 3557, 6376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16192) [M::mem_pestat] mean and std.dev: (4049.95, 2850.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21100) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1387, 3361, 6162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15712) [M::mem_pestat] mean and std.dev: (3919.57, 2839.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20487) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (200000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 362.701 CPU sec, 23.201 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7529, 406662, 6899, 7304) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1246, 3236, 6224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16180) [M::mem_pestat] mean and std.dev: (3830.44, 2896.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21158) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 212, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (32, 392) [M::mem_pestat] mean and std.dev: (210.34, 51.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 464) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1445, 3441, 6187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15671) [M::mem_pestat] mean and std.dev: (3971.43, 2827.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20413) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1399, 3333, 6339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16219) [M::mem_pestat] mean and std.dev: (3951.57, 2867.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21159) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 362.026 CPU sec, 23.189 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7391, 406659, 6875, 7290) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1209, 3142, 6136) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15990) [M::mem_pestat] mean and std.dev: (3790.73, 2896.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20917) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 211, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (32, 392) [M::mem_pestat] mean and std.dev: (210.30, 51.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 464) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1447, 3476, 6254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15868) [M::mem_pestat] mean and std.dev: (3989.41, 2828.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20675) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1371, 3399, 6321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16221) [M::mem_pestat] mean and std.dev: (3966.21, 2865.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21171) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 361.663 CPU sec, 23.267 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7287, 407371, 6959, 7205) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1287, 3267, 6191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15999) [M::mem_pestat] mean and std.dev: (3848.17, 2887.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20903) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 211, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (34, 389) [M::mem_pestat] mean and std.dev: (210.11, 51.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 460) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1422, 3391, 6193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15735) [M::mem_pestat] mean and std.dev: (3962.22, 2843.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20506) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1386, 3284, 6190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15798) [M::mem_pestat] mean and std.dev: (3903.11, 2834.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20602) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (200000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 363.006 CPU sec, 23.124 real sec [M::process] read 75060 sequences (9382500 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7667, 406070, 6861, 7221) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1227, 3190, 6147) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15987) [M::mem_pestat] mean and std.dev: (3783.82, 2886.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20907) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 211, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (34, 389) [M::mem_pestat] mean and std.dev: (210.08, 51.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 460) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1457, 3521, 6305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16001) [M::mem_pestat] mean and std.dev: (4011.44, 2848.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20849) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1381, 3283, 6146) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15676) [M::mem_pestat] mean and std.dev: (3879.43, 2825.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20441) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 359.470 CPU sec, 23.194 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (369, 19005, 319, 343) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1117, 3230, 6361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16849) [M::mem_pestat] mean and std.dev: (3855.98, 2952.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 22093) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 211, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (34, 389) [M::mem_pestat] mean and std.dev: (209.93, 51.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 460) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1524, 3528, 6242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15678) [M::mem_pestat] mean and std.dev: (3991.93, 2858.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20396) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1254, 3452, 6519) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 17049) [M::mem_pestat] mean and std.dev: (3972.06, 2932.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 22314) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 75060 reads in 18.254 CPU sec, 1.180 real sec [main] Version: 0.7.17-r1188 [main] CMD: bwa mem -SP5M -t 20 /oak/stanford/groups/akundaje/marinovg/genomes/Ciliates/Tetrahymena_thermophila_SB210-E-JCVI_TeTherMIC_3/bwa-indexes/Tetrahymena_thermophila_SB210-E-JCVI_TeTherMIC_3.fa /oak/stanford/groups/akundaje/marinovg/papers/2022_Tetrahymena-Hi-C/2022-05-24-figures/juicer-MAC_24_hpm_Hi-C-GSM4161207-MIC/splits/Reads_R1.fastq.gz /oak/stanford/groups/akundaje/marinovg/papers/2022_Tetrahymena-Hi-C/2022-05-24-figures/juicer-MAC_24_hpm_Hi-C-GSM4161207-MIC/splits/Reads_R2.fastq.gz [main] Real time: 1872.298 sec; CPU: 28334.382 sec Picked up _JAVA_OPTIONS: -Xmx16384m Picked up _JAVA_OPTIONS: -Xmx16384m Picked up _JAVA_OPTIONS: -Xmx16384m Picked up _JAVA_OPTIONS: -Xmx16384m