[M::bwa_idx_load_from_disk] read 0 ALT contigs [M::process] read 5555556 sequences (200000016 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27578, 945829, 25892, 27212) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1278, 3266, 6141) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15867) [M::mem_pestat] mean and std.dev: (3869.02, 2885.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20730) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (125, 173, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 482) [M::mem_pestat] mean and std.dev: (186.70, 84.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 601) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1542, 3545, 6328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15900) [M::mem_pestat] mean and std.dev: (4059.46, 2830.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20686) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1300, 3311, 6152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15856) [M::mem_pestat] mean and std.dev: (3880.71, 2871.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20708) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 363.617 CPU sec, 51.562 real sec [M::process] read 5555556 sequences (200000016 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27759, 945944, 26103, 27517) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1251, 3229, 6108) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15822) [M::mem_pestat] mean and std.dev: (3840.22, 2882.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20679) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (125, 173, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 482) [M::mem_pestat] mean and std.dev: (186.56, 84.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 601) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1536, 3519, 6313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15867) [M::mem_pestat] mean and std.dev: (4050.59, 2835.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20644) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1266, 3276, 6185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16023) [M::mem_pestat] mean and std.dev: (3871.15, 2882.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20942) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 338.166 CPU sec, 31.270 real sec [M::process] read 5555556 sequences (200000016 bp)... [M::process] read 5555556 sequences (200000016 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27396, 946863, 26350, 27208) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1290, 3289, 6176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15948) [M::mem_pestat] mean and std.dev: (3882.96, 2887.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20834) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (125, 173, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 479) [M::mem_pestat] mean and std.dev: (186.16, 84.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 597) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1507, 3481, 6321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15949) [M::mem_pestat] mean and std.dev: (4039.67, 2833.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20763) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1315, 3304, 6165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15865) [M::mem_pestat] mean and std.dev: (3883.61, 2866.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20715) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 320.888 CPU sec, 21.352 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27436, 944153, 26179, 27196) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1268, 3279, 6121) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15827) [M::mem_pestat] mean and std.dev: (3855.12, 2870.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20680) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (125, 172, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 479) [M::mem_pestat] mean and std.dev: (186.06, 84.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 597) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1570, 3517, 6334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15862) [M::mem_pestat] mean and std.dev: (4069.95, 2828.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20626) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1259, 3230, 6105) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15797) [M::mem_pestat] mean and std.dev: (3845.67, 2875.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20643) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 325.932 CPU sec, 28.209 real sec [M::process] read 5555556 sequences (200000016 bp)... [M::process] read 5555556 sequences (200000016 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27437, 945665, 26500, 27468) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1274, 3249, 6145) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15887) [M::mem_pestat] mean and std.dev: (3855.37, 2870.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20758) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (125, 173, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 482) [M::mem_pestat] mean and std.dev: (186.53, 84.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 601) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1509, 3491, 6296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15870) [M::mem_pestat] mean and std.dev: (4036.06, 2833.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20657) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1264, 3271, 6147) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15913) [M::mem_pestat] mean and std.dev: (3861.95, 2875.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20796) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 327.876 CPU sec, 32.654 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27467, 943657, 26519, 27308) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1247, 3209, 6138) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15920) [M::mem_pestat] mean and std.dev: (3845.30, 2899.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20811) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (125, 173, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 479) [M::mem_pestat] mean and std.dev: (186.48, 84.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 597) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1513, 3519, 6366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16072) [M::mem_pestat] mean and std.dev: (4056.44, 2845.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20925) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1296, 3271, 6209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16035) [M::mem_pestat] mean and std.dev: (3890.35, 2893.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20948) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 351.027 CPU sec, 40.165 real sec [M::process] read 5555556 sequences (200000016 bp)... [M::process] read 5555556 sequences (200000016 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27620, 945411, 26355, 27427) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1236, 3223, 6136) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15936) [M::mem_pestat] mean and std.dev: (3836.71, 2885.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20836) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (125, 173, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 479) [M::mem_pestat] mean and std.dev: (186.33, 84.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 597) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1537, 3489, 6290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15796) [M::mem_pestat] mean and std.dev: (4045.21, 2834.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20549) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1270, 3279, 6143) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15889) [M::mem_pestat] mean and std.dev: (3862.12, 2874.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20762) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 330.313 CPU sec, 32.520 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27482, 946236, 26467, 27415) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1244, 3216, 6170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16022) [M::mem_pestat] mean and std.dev: (3844.45, 2890.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20948) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (125, 172, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 476) [M::mem_pestat] mean and std.dev: (185.73, 84.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1534, 3499, 6318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15886) [M::mem_pestat] mean and std.dev: (4048.64, 2835.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20670) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1281, 3243, 6194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16020) [M::mem_pestat] mean and std.dev: (3868.62, 2890.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20933) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 351.413 CPU sec, 39.280 real sec [M::process] read 5555556 sequences (200000016 bp)... [M::process] read 5555556 sequences (200000016 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27428, 944792, 25938, 27032) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1243, 3210, 6119) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15871) [M::mem_pestat] mean and std.dev: (3830.08, 2881.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20747) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (125, 173, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 479) [M::mem_pestat] mean and std.dev: (186.15, 84.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 597) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1493, 3477, 6325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15989) [M::mem_pestat] mean and std.dev: (4036.64, 2850.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20821) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1291, 3261, 6162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15904) [M::mem_pestat] mean and std.dev: (3876.20, 2878.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20775) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 316.699 CPU sec, 20.777 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27412, 947098, 26408, 27147) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1270, 3224, 6083) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15709) [M::mem_pestat] mean and std.dev: (3832.60, 2864.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20522) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (125, 172, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 479) [M::mem_pestat] mean and std.dev: (186.14, 84.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 597) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1517, 3476, 6265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15761) [M::mem_pestat] mean and std.dev: (4032.12, 2834.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20509) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1301, 3276, 6181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15941) [M::mem_pestat] mean and std.dev: (3887.74, 2893.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20821) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 311.225 CPU sec, 20.602 real sec [M::process] read 5555556 sequences (200000016 bp)... [M::process] read 5555556 sequences (200000016 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27470, 946766, 26383, 27164) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1277, 3284, 6187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16007) [M::mem_pestat] mean and std.dev: (3885.77, 2883.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20917) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (125, 173, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 479) [M::mem_pestat] mean and std.dev: (186.26, 84.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 597) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1532, 3501, 6277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15767) [M::mem_pestat] mean and std.dev: (4033.43, 2823.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20512) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1272, 3285, 6182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16002) [M::mem_pestat] mean and std.dev: (3881.10, 2885.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20912) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 326.049 CPU sec, 30.910 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27362, 947071, 26326, 27458) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1240, 3172, 6140) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15940) [M::mem_pestat] mean and std.dev: (3827.77, 2889.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20840) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (125, 172, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 479) [M::mem_pestat] mean and std.dev: (185.91, 84.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 597) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1503, 3528, 6345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16029) [M::mem_pestat] mean and std.dev: (4059.93, 2857.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20871) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1295, 3254, 6237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16121) [M::mem_pestat] mean and std.dev: (3877.04, 2887.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21063) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 347.419 CPU sec, 38.677 real sec [M::process] read 5555556 sequences (200000016 bp)... [M::process] read 5555556 sequences (200000016 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27360, 947624, 26096, 27483) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1266, 3243, 6156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15936) [M::mem_pestat] mean and std.dev: (3859.64, 2881.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20826) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (125, 172, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 476) [M::mem_pestat] mean and std.dev: (185.49, 83.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1512, 3514, 6299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15873) [M::mem_pestat] mean and std.dev: (4050.73, 2838.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20660) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1253, 3234, 6156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15962) [M::mem_pestat] mean and std.dev: (3852.87, 2889.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20865) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 324.984 CPU sec, 31.861 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27782, 944881, 26370, 27557) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1253, 3216, 6131) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15887) [M::mem_pestat] mean and std.dev: (3841.02, 2887.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20765) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (125, 172, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 476) [M::mem_pestat] mean and std.dev: (185.64, 84.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1502, 3485, 6317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15947) [M::mem_pestat] mean and std.dev: (4043.15, 2850.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20762) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1272, 3239, 6179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15993) [M::mem_pestat] mean and std.dev: (3873.38, 2892.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20900) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 350.523 CPU sec, 39.233 real sec [M::process] read 5555556 sequences (200000016 bp)... [M::process] read 5555556 sequences (200000016 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27445, 945789, 26699, 27187) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1259, 3220, 6150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15932) [M::mem_pestat] mean and std.dev: (3848.40, 2888.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20823) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (125, 172, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 479) [M::mem_pestat] mean and std.dev: (186.09, 84.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 597) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1508, 3456, 6260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15764) [M::mem_pestat] mean and std.dev: (4008.51, 2815.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20516) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1297, 3317, 6216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16054) [M::mem_pestat] mean and std.dev: (3901.14, 2890.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20973) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 316.111 CPU sec, 20.767 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27404, 945791, 25965, 27397) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1273, 3236, 6152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15910) [M::mem_pestat] mean and std.dev: (3845.79, 2878.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20789) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (125, 172, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 479) [M::mem_pestat] mean and std.dev: (185.90, 84.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 597) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1531, 3523, 6345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15973) [M::mem_pestat] mean and std.dev: (4066.58, 2844.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20787) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1275, 3276, 6153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15909) [M::mem_pestat] mean and std.dev: (3880.47, 2892.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20787) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 313.989 CPU sec, 21.135 real sec [M::process] read 5555556 sequences (200000016 bp)... [M::process] read 5555556 sequences (200000016 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27517, 945986, 26398, 27472) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1303, 3307, 6211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16027) [M::mem_pestat] mean and std.dev: (3897.82, 2889.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20935) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (125, 172, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 476) [M::mem_pestat] mean and std.dev: (185.58, 83.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1526, 3495, 6281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15791) [M::mem_pestat] mean and std.dev: (4034.33, 2824.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20546) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1275, 3255, 6169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15957) [M::mem_pestat] mean and std.dev: (3868.51, 2887.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20851) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 327.123 CPU sec, 32.076 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27287, 945959, 26391, 27327) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1268, 3286, 6201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16067) [M::mem_pestat] mean and std.dev: (3870.33, 2883.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21000) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 172, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (185.48, 84.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 596) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1527, 3479, 6358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16020) [M::mem_pestat] mean and std.dev: (4045.89, 2847.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20851) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1274, 3260, 6165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15947) [M::mem_pestat] mean and std.dev: (3875.90, 2891.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20838) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 355.687 CPU sec, 40.882 real sec [M::process] read 5555556 sequences (200000016 bp)... [M::process] read 5555556 sequences (200000016 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27361, 945541, 26565, 27319) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1282, 3282, 6176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15964) [M::mem_pestat] mean and std.dev: (3870.81, 2880.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20858) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (126, 174, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 483) [M::mem_pestat] mean and std.dev: (187.75, 85.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1568, 3516, 6272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15680) [M::mem_pestat] mean and std.dev: (4049.79, 2815.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20384) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1277, 3252, 6153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15905) [M::mem_pestat] mean and std.dev: (3860.22, 2882.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20781) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 331.192 CPU sec, 31.659 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28029, 943075, 26186, 27180) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1269, 3290, 6236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16170) [M::mem_pestat] mean and std.dev: (3890.80, 2901.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21137) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (126, 174, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (188.27, 85.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1529, 3495, 6250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15692) [M::mem_pestat] mean and std.dev: (4030.16, 2815.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20413) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1307, 3302, 6169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15893) [M::mem_pestat] mean and std.dev: (3882.09, 2870.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20755) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 340.274 CPU sec, 33.698 real sec [M::process] read 5555556 sequences (200000016 bp)... [M::process] read 5555556 sequences (200000016 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27584, 946119, 26318, 27474) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1264, 3219, 6147) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15913) [M::mem_pestat] mean and std.dev: (3849.46, 2882.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20796) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (126, 174, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (188.13, 85.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1502, 3488, 6243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15725) [M::mem_pestat] mean and std.dev: (4016.98, 2822.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20466) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1262, 3251, 6176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16004) [M::mem_pestat] mean and std.dev: (3867.16, 2888.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20918) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 314.848 CPU sec, 20.856 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27722, 944887, 26255, 27710) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1279, 3242, 6151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15895) [M::mem_pestat] mean and std.dev: (3856.33, 2886.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20767) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (126, 174, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (188.18, 85.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1537, 3517, 6304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15838) [M::mem_pestat] mean and std.dev: (4051.50, 2826.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20605) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1285, 3244, 6179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15967) [M::mem_pestat] mean and std.dev: (3875.45, 2886.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20861) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 315.397 CPU sec, 22.416 real sec [M::process] read 5555556 sequences (200000016 bp)... [M::process] read 5555556 sequences (200000016 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27478, 944776, 26671, 27407) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1282, 3239, 6177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15967) [M::mem_pestat] mean and std.dev: (3865.68, 2890.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20862) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (126, 175, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (188.63, 85.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1526, 3513, 6365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16043) [M::mem_pestat] mean and std.dev: (4068.31, 2851.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20882) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1245, 3213, 6143) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15939) [M::mem_pestat] mean and std.dev: (3842.02, 2889.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20837) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 324.359 CPU sec, 31.584 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27435, 945093, 26154, 27421) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1273, 3237, 6150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15904) [M::mem_pestat] mean and std.dev: (3864.49, 2887.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20781) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (126, 174, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (188.20, 85.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1538, 3507, 6288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15788) [M::mem_pestat] mean and std.dev: (4041.82, 2832.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20538) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1291, 3286, 6180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15958) [M::mem_pestat] mean and std.dev: (3883.94, 2886.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20847) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 348.360 CPU sec, 39.326 real sec [M::process] read 5555556 sequences (200000016 bp)... [M::process] read 5555556 sequences (200000016 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27589, 946106, 26388, 27518) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1276, 3299, 6178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15982) [M::mem_pestat] mean and std.dev: (3877.21, 2878.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20884) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (126, 174, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (188.11, 85.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1522, 3473, 6296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15844) [M::mem_pestat] mean and std.dev: (4030.79, 2831.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20618) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1314, 3278, 6154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15834) [M::mem_pestat] mean and std.dev: (3884.82, 2865.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20674) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 327.543 CPU sec, 30.688 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27501, 947477, 26335, 27531) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1285, 3242, 6128) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15814) [M::mem_pestat] mean and std.dev: (3850.05, 2877.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20657) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (126, 174, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (188.00, 85.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1546, 3518, 6344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15940) [M::mem_pestat] mean and std.dev: (4063.42, 2835.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20738) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1289, 3262, 6215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16067) [M::mem_pestat] mean and std.dev: (3885.48, 2896.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20993) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 353.714 CPU sec, 38.448 real sec [M::process] read 5555556 sequences (200000016 bp)... [M::process] read 5555556 sequences (200000016 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27448, 946326, 26485, 27572) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1244, 3216, 6126) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15890) [M::mem_pestat] mean and std.dev: (3830.09, 2877.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20772) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (126, 174, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 483) [M::mem_pestat] mean and std.dev: (187.88, 85.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1572, 3556, 6362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15942) [M::mem_pestat] mean and std.dev: (4085.07, 2831.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20732) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1308, 3328, 6248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16128) [M::mem_pestat] mean and std.dev: (3910.48, 2888.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21068) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 326.005 CPU sec, 30.653 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27777, 946967, 26518, 27229) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1241, 3223, 6152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15974) [M::mem_pestat] mean and std.dev: (3846.12, 2888.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20885) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (126, 174, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 483) [M::mem_pestat] mean and std.dev: (187.75, 84.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1534, 3507, 6259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15709) [M::mem_pestat] mean and std.dev: (4039.72, 2825.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20434) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1265, 3229, 6159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15947) [M::mem_pestat] mean and std.dev: (3848.78, 2884.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20841) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 352.592 CPU sec, 39.842 real sec [M::process] read 5555556 sequences (200000016 bp)... [M::process] read 5555556 sequences (200000016 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27596, 944874, 26540, 27427) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1272, 3249, 6097) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15747) [M::mem_pestat] mean and std.dev: (3844.47, 2872.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20572) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (126, 174, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (188.21, 85.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1520, 3510, 6301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15863) [M::mem_pestat] mean and std.dev: (4044.80, 2836.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20644) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1311, 3292, 6208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16002) [M::mem_pestat] mean and std.dev: (3893.67, 2879.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20899) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 329.723 CPU sec, 31.969 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27629, 944299, 26517, 27585) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1272, 3225, 6135) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15861) [M::mem_pestat] mean and std.dev: (3852.22, 2885.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20724) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (126, 174, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (187.97, 85.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1538, 3522, 6293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15803) [M::mem_pestat] mean and std.dev: (4048.70, 2828.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20558) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1286, 3281, 6168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15932) [M::mem_pestat] mean and std.dev: (3877.88, 2885.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20814) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 354.154 CPU sec, 39.260 real sec [M::process] read 5555556 sequences (200000016 bp)... [M::process] read 5555556 sequences (200000016 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27489, 944610, 26562, 27363) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1246, 3243, 6109) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15835) [M::mem_pestat] mean and std.dev: (3839.07, 2878.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20698) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (126, 174, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 483) [M::mem_pestat] mean and std.dev: (187.58, 84.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1535, 3496, 6339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15947) [M::mem_pestat] mean and std.dev: (4060.03, 2845.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20751) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1284, 3343, 6218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16086) [M::mem_pestat] mean and std.dev: (3903.02, 2894.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21020) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 330.692 CPU sec, 31.279 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25432, 928282, 24137, 25027) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (851, 2751, 5788) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15662) [M::mem_pestat] mean and std.dev: (3511.64, 2932.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20599) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 147, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 374) [M::mem_pestat] mean and std.dev: (157.89, 62.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 461) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1001, 2930, 5860) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15578) [M::mem_pestat] mean and std.dev: (3614.33, 2917.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20437) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (849, 2687, 5721) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15465) [M::mem_pestat] mean and std.dev: (3484.47, 2931.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20337) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 388.061 CPU sec, 43.435 real sec [M::process] read 5555556 sequences (200000016 bp)... [M::process] read 5555556 sequences (200000016 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24683, 921005, 23660, 24386) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (736, 2485, 5626) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15406) [M::mem_pestat] mean and std.dev: (3372.64, 2948.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20296) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 141, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 335) [M::mem_pestat] mean and std.dev: (149.15, 54.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 410) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (841, 2710, 5724) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15490) [M::mem_pestat] mean and std.dev: (3479.72, 2937.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20373) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (738, 2506, 5590) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15294) [M::mem_pestat] mean and std.dev: (3367.18, 2939.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20146) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 350.651 CPU sec, 23.149 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24689, 924089, 23432, 24423) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (711, 2463, 5661) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15561) [M::mem_pestat] mean and std.dev: (3363.92, 2958.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20511) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 141, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 335) [M::mem_pestat] mean and std.dev: (148.99, 53.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 410) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (835, 2703, 5771) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15643) [M::mem_pestat] mean and std.dev: (3479.09, 2935.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20579) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (725, 2490, 5576) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15278) [M::mem_pestat] mean and std.dev: (3348.93, 2927.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20129) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 360.134 CPU sec, 26.242 real sec [M::process] read 5555556 sequences (200000016 bp)... [M::process] read 5555556 sequences (200000016 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24900, 922386, 23316, 24332) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (699, 2396, 5531) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15195) [M::mem_pestat] mean and std.dev: (3315.00, 2936.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20027) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 140, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 335) [M::mem_pestat] mean and std.dev: (148.93, 53.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 410) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (795, 2613, 5739) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15627) [M::mem_pestat] mean and std.dev: (3450.92, 2951.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20571) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (743, 2530, 5654) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15476) [M::mem_pestat] mean and std.dev: (3387.97, 2942.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20387) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 376.884 CPU sec, 40.179 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24375, 923107, 23572, 24225) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (720, 2432, 5514) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15102) [M::mem_pestat] mean and std.dev: (3323.20, 2927.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19896) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 140, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 335) [M::mem_pestat] mean and std.dev: (148.96, 53.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 410) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (824, 2690, 5780) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15692) [M::mem_pestat] mean and std.dev: (3481.69, 2953.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20648) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (747, 2532, 5582) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15252) [M::mem_pestat] mean and std.dev: (3366.26, 2920.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20087) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 378.416 CPU sec, 35.698 real sec [M::process] read 5555556 sequences (200000016 bp)... [M::process] read 5555556 sequences (200000016 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24337, 921558, 23620, 24612) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (731, 2479, 5574) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15260) [M::mem_pestat] mean and std.dev: (3345.13, 2936.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20103) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 141, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 335) [M::mem_pestat] mean and std.dev: (149.15, 54.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 410) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (819, 2630, 5710) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15492) [M::mem_pestat] mean and std.dev: (3453.09, 2942.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20383) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (748, 2562, 5635) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15409) [M::mem_pestat] mean and std.dev: (3394.50, 2938.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20296) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 354.640 CPU sec, 23.361 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24581, 925328, 23528, 24602) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (727, 2495, 5624) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15418) [M::mem_pestat] mean and std.dev: (3366.36, 2947.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20315) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 140, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 335) [M::mem_pestat] mean and std.dev: (148.94, 53.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 410) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (854, 2679, 5790) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15662) [M::mem_pestat] mean and std.dev: (3490.31, 2951.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20598) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (735, 2516, 5614) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15372) [M::mem_pestat] mean and std.dev: (3375.56, 2936.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20251) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 357.466 CPU sec, 26.189 real sec [M::process] read 5555556 sequences (200000016 bp)... [M::process] read 5555556 sequences (200000016 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24605, 923952, 23311, 24785) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (723, 2494, 5640) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15474) [M::mem_pestat] mean and std.dev: (3375.67, 2962.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20391) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 141, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 335) [M::mem_pestat] mean and std.dev: (149.00, 53.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 410) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (843, 2720, 5818) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15768) [M::mem_pestat] mean and std.dev: (3494.82, 2949.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20743) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (722, 2434, 5523) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15125) [M::mem_pestat] mean and std.dev: (3324.83, 2920.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19926) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 353.543 CPU sec, 23.405 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24583, 923661, 23516, 24332) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (719, 2422, 5503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15071) [M::mem_pestat] mean and std.dev: (3318.65, 2915.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19855) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 140, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 335) [M::mem_pestat] mean and std.dev: (149.00, 53.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 410) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (827, 2690, 5770) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15656) [M::mem_pestat] mean and std.dev: (3479.10, 2954.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20599) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (739, 2509, 5604) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15334) [M::mem_pestat] mean and std.dev: (3360.56, 2931.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20199) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 355.908 CPU sec, 24.372 real sec [M::process] read 5555556 sequences (200000016 bp)... [M::process] read 5555556 sequences (200000016 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24529, 923552, 23240, 24318) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (726, 2510, 5720) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15708) [M::mem_pestat] mean and std.dev: (3388.83, 2965.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20702) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 141, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 335) [M::mem_pestat] mean and std.dev: (149.00, 53.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 410) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (839, 2716, 5813) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15761) [M::mem_pestat] mean and std.dev: (3506.62, 2961.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20735) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (720, 2439, 5575) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15285) [M::mem_pestat] mean and std.dev: (3342.20, 2942.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20140) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 350.321 CPU sec, 23.196 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24564, 926059, 23513, 24229) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (706, 2436, 5510) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15118) [M::mem_pestat] mean and std.dev: (3313.46, 2922.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19922) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 140, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 335) [M::mem_pestat] mean and std.dev: (148.93, 53.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 410) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (855, 2726, 5743) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15519) [M::mem_pestat] mean and std.dev: (3483.71, 2929.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20407) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (753, 2508, 5563) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15183) [M::mem_pestat] mean and std.dev: (3364.90, 2929.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19993) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 354.164 CPU sec, 24.351 real sec [M::process] read 5555556 sequences (200000016 bp)... [M::process] read 5555556 sequences (200000016 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24253, 926073, 23377, 24711) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (739, 2501, 5548) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15166) [M::mem_pestat] mean and std.dev: (3350.64, 2916.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19975) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 140, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 335) [M::mem_pestat] mean and std.dev: (148.76, 53.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 410) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (791, 2625, 5749) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15665) [M::mem_pestat] mean and std.dev: (3446.26, 2956.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20623) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (747, 2480, 5544) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15138) [M::mem_pestat] mean and std.dev: (3346.64, 2916.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19935) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 380.400 CPU sec, 42.135 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24645, 925945, 23448, 24382) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (735, 2494, 5598) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15324) [M::mem_pestat] mean and std.dev: (3362.35, 2938.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20187) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 140, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 335) [M::mem_pestat] mean and std.dev: (148.87, 53.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 410) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (852, 2735, 5836) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15804) [M::mem_pestat] mean and std.dev: (3523.84, 2961.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20788) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (735, 2496, 5634) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15432) [M::mem_pestat] mean and std.dev: (3367.88, 2939.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20331) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 381.172 CPU sec, 36.733 real sec [M::process] read 5555556 sequences (200000016 bp)... [M::process] read 5555556 sequences (200000016 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24777, 924584, 23549, 24404) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (714, 2465, 5591) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15345) [M::mem_pestat] mean and std.dev: (3347.85, 2941.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20222) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 140, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 335) [M::mem_pestat] mean and std.dev: (148.90, 53.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 410) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (841, 2657, 5762) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15604) [M::mem_pestat] mean and std.dev: (3471.02, 2944.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20525) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (720, 2467, 5575) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15285) [M::mem_pestat] mean and std.dev: (3343.75, 2931.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20140) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 380.458 CPU sec, 42.253 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24719, 924837, 23609, 24457) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (725, 2502, 5581) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15293) [M::mem_pestat] mean and std.dev: (3352.89, 2934.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20149) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 140, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 335) [M::mem_pestat] mean and std.dev: (148.90, 53.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 410) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (831, 2682, 5792) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15714) [M::mem_pestat] mean and std.dev: (3484.64, 2948.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20675) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (732, 2529, 5588) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15300) [M::mem_pestat] mean and std.dev: (3362.59, 2932.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20156) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 383.428 CPU sec, 38.206 real sec [M::process] read 5555556 sequences (200000016 bp)... [M::process] read 5555556 sequences (200000016 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24667, 925973, 23334, 24112) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (720, 2507, 5582) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15306) [M::mem_pestat] mean and std.dev: (3359.13, 2940.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20168) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 140, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 335) [M::mem_pestat] mean and std.dev: (148.84, 53.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 410) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (835, 2733, 5806) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15748) [M::mem_pestat] mean and std.dev: (3504.04, 2957.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20719) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (749, 2527, 5656) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15470) [M::mem_pestat] mean and std.dev: (3387.74, 2944.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20377) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 380.116 CPU sec, 41.751 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24538, 922099, 23587, 24536) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (732, 2478, 5594) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15318) [M::mem_pestat] mean and std.dev: (3353.53, 2933.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20180) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 140, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 335) [M::mem_pestat] mean and std.dev: (148.85, 53.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 410) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (813, 2597, 5673) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15393) [M::mem_pestat] mean and std.dev: (3429.00, 2928.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20253) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (736, 2488, 5592) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15304) [M::mem_pestat] mean and std.dev: (3365.44, 2939.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20160) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 379.644 CPU sec, 36.447 real sec [M::process] read 5555556 sequences (200000016 bp)... [M::process] read 5555556 sequences (200000016 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24173, 921484, 23361, 24262) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (697, 2446, 5549) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15253) [M::mem_pestat] mean and std.dev: (3326.02, 2934.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20105) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 140, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 332) [M::mem_pestat] mean and std.dev: (148.56, 53.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 406) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (845, 2667, 5772) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15626) [M::mem_pestat] mean and std.dev: (3486.16, 2953.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20553) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (736, 2473, 5613) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15367) [M::mem_pestat] mean and std.dev: (3362.60, 2933.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20244) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 378.785 CPU sec, 41.669 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24220, 922402, 23197, 24238) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (722, 2467, 5497) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15047) [M::mem_pestat] mean and std.dev: (3325.79, 2915.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19822) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 140, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 335) [M::mem_pestat] mean and std.dev: (148.83, 53.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 410) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (827, 2663, 5695) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15431) [M::mem_pestat] mean and std.dev: (3443.14, 2921.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20299) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (758, 2522, 5624) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15356) [M::mem_pestat] mean and std.dev: (3381.27, 2941.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20222) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 380.272 CPU sec, 36.225 real sec [M::process] read 5555556 sequences (200000016 bp)... [M::process] read 5555556 sequences (200000016 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24232, 923735, 23182, 24491) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (735, 2539, 5622) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15396) [M::mem_pestat] mean and std.dev: (3377.00, 2935.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20283) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 140, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 332) [M::mem_pestat] mean and std.dev: (148.59, 53.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 406) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (799, 2660, 5769) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15709) [M::mem_pestat] mean and std.dev: (3467.01, 2955.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20679) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (715, 2474, 5609) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15397) [M::mem_pestat] mean and std.dev: (3350.36, 2938.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20291) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 376.412 CPU sec, 40.983 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24326, 926125, 23161, 24218) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (704, 2441, 5601) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15395) [M::mem_pestat] mean and std.dev: (3337.16, 2944.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20292) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 140, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 332) [M::mem_pestat] mean and std.dev: (148.25, 53.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 406) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (819, 2634, 5715) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15507) [M::mem_pestat] mean and std.dev: (3445.74, 2936.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20403) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (742, 2490, 5601) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15319) [M::mem_pestat] mean and std.dev: (3370.92, 2948.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20178) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 377.850 CPU sec, 35.415 real sec [M::process] read 5555556 sequences (200000016 bp)... [M::process] read 5555556 sequences (200000016 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24616, 922375, 23184, 24340) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (732, 2474, 5527) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15117) [M::mem_pestat] mean and std.dev: (3340.24, 2933.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19912) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 140, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 332) [M::mem_pestat] mean and std.dev: (148.46, 53.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 406) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (823, 2620, 5738) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15568) [M::mem_pestat] mean and std.dev: (3458.27, 2952.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20483) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (757, 2533, 5581) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15229) [M::mem_pestat] mean and std.dev: (3373.54, 2925.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20053) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 353.388 CPU sec, 23.410 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24228, 922303, 23050, 24512) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (706, 2457, 5536) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15196) [M::mem_pestat] mean and std.dev: (3326.03, 2931.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20026) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 140, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 332) [M::mem_pestat] mean and std.dev: (148.48, 53.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 406) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (810, 2655, 5708) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15504) [M::mem_pestat] mean and std.dev: (3450.81, 2937.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20402) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (752, 2488, 5579) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15233) [M::mem_pestat] mean and std.dev: (3370.08, 2936.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20060) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 357.536 CPU sec, 25.095 real sec [M::process] read 5555556 sequences (200000016 bp)... [M::process] read 5555556 sequences (200000016 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24419, 924188, 23146, 24398) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (725, 2471, 5543) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15179) [M::mem_pestat] mean and std.dev: (3335.97, 2918.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19997) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 140, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 332) [M::mem_pestat] mean and std.dev: (148.47, 53.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 406) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (816, 2672, 5795) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15753) [M::mem_pestat] mean and std.dev: (3475.64, 2949.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20732) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (724, 2488, 5506) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15070) [M::mem_pestat] mean and std.dev: (3337.09, 2927.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19852) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 355.407 CPU sec, 23.712 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24367, 924179, 23188, 24498) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (744, 2520, 5601) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15315) [M::mem_pestat] mean and std.dev: (3371.05, 2933.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20172) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 140, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 332) [M::mem_pestat] mean and std.dev: (148.48, 53.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 406) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (832, 2665, 5735) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15541) [M::mem_pestat] mean and std.dev: (3460.80, 2936.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20444) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (756, 2510, 5558) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15162) [M::mem_pestat] mean and std.dev: (3357.71, 2924.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19964) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 351.550 CPU sec, 24.523 real sec [M::process] read 5555556 sequences (200000016 bp)... [M::process] read 5555556 sequences (200000016 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24418, 921999, 23395, 24539) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (712, 2484, 5622) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15442) [M::mem_pestat] mean and std.dev: (3350.72, 2943.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20352) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 140, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 335) [M::mem_pestat] mean and std.dev: (148.75, 53.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 410) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (809, 2642, 5735) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15587) [M::mem_pestat] mean and std.dev: (3458.42, 2954.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20513) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (739, 2518, 5597) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15313) [M::mem_pestat] mean and std.dev: (3369.65, 2934.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20171) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 352.685 CPU sec, 24.947 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24197, 923489, 23530, 24770) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (713, 2487, 5593) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15353) [M::mem_pestat] mean and std.dev: (3349.60, 2941.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20233) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 140, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 332) [M::mem_pestat] mean and std.dev: (148.51, 53.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 406) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (842, 2748, 5800) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15716) [M::mem_pestat] mean and std.dev: (3499.22, 2941.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20674) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (745, 2515, 5668) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15514) [M::mem_pestat] mean and std.dev: (3389.44, 2944.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20437) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 353.367 CPU sec, 23.705 real sec [M::process] read 5555556 sequences (200000016 bp)... [M::process] read 5555556 sequences (200000016 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24446, 926397, 23080, 24399) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (718, 2447, 5578) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15298) [M::mem_pestat] mean and std.dev: (3342.37, 2940.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20158) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 140, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 332) [M::mem_pestat] mean and std.dev: (148.28, 53.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 406) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (813, 2626, 5723) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15543) [M::mem_pestat] mean and std.dev: (3446.82, 2944.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20453) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (744, 2511, 5529) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15099) [M::mem_pestat] mean and std.dev: (3350.19, 2914.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19884) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 375.284 CPU sec, 40.175 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24417, 924491, 23367, 24080) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (729, 2484, 5577) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15273) [M::mem_pestat] mean and std.dev: (3344.32, 2933.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20121) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 140, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 332) [M::mem_pestat] mean and std.dev: (148.51, 53.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 406) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (847, 2697, 5815) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15751) [M::mem_pestat] mean and std.dev: (3492.75, 2949.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20719) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (709, 2446, 5583) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15331) [M::mem_pestat] mean and std.dev: (3337.32, 2937.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20205) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 381.132 CPU sec, 36.281 real sec [M::process] read 5555556 sequences (200000016 bp)... [M::process] read 5555556 sequences (200000016 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24016, 923710, 23330, 24039) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (723, 2484, 5570) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15264) [M::mem_pestat] mean and std.dev: (3349.72, 2935.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20111) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 140, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 332) [M::mem_pestat] mean and std.dev: (148.46, 53.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 406) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (820, 2686, 5792) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15736) [M::mem_pestat] mean and std.dev: (3480.61, 2962.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20708) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (731, 2458, 5520) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15098) [M::mem_pestat] mean and std.dev: (3328.93, 2922.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19887) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 378.241 CPU sec, 39.891 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24288, 927161, 23270, 24307) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (725, 2465, 5596) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15338) [M::mem_pestat] mean and std.dev: (3348.49, 2932.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20209) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 140, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 332) [M::mem_pestat] mean and std.dev: (148.41, 53.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 406) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (841, 2699, 5770) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15628) [M::mem_pestat] mean and std.dev: (3474.23, 2937.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20557) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (760, 2552, 5601) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15283) [M::mem_pestat] mean and std.dev: (3380.77, 2927.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20124) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 380.629 CPU sec, 35.483 real sec [M::process] read 5555556 sequences (200000016 bp)... [M::process] read 5555556 sequences (200000016 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24071, 926185, 23396, 24253) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (734, 2520, 5629) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15419) [M::mem_pestat] mean and std.dev: (3371.45, 2941.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20314) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 140, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 332) [M::mem_pestat] mean and std.dev: (148.47, 53.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 406) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (819, 2628, 5651) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15315) [M::mem_pestat] mean and std.dev: (3431.59, 2918.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20147) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (754, 2481, 5576) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15220) [M::mem_pestat] mean and std.dev: (3357.10, 2921.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20042) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 352.348 CPU sec, 23.227 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24541, 927693, 23234, 24357) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (732, 2490, 5606) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15354) [M::mem_pestat] mean and std.dev: (3354.67, 2933.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20228) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 140, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 332) [M::mem_pestat] mean and std.dev: (148.49, 53.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 406) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (835, 2704, 5784) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15682) [M::mem_pestat] mean and std.dev: (3482.44, 2937.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20631) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (731, 2520, 5650) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15488) [M::mem_pestat] mean and std.dev: (3382.97, 2951.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20407) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 357.774 CPU sec, 26.359 real sec [M::process] read 5555556 sequences (200000016 bp)... [M::process] read 5555556 sequences (200000016 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24298, 925623, 23243, 24138) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (719, 2471, 5567) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15263) [M::mem_pestat] mean and std.dev: (3355.22, 2950.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20111) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 140, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 332) [M::mem_pestat] mean and std.dev: (148.39, 53.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 406) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (824, 2652, 5749) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15599) [M::mem_pestat] mean and std.dev: (3462.47, 2936.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20524) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (736, 2513, 5575) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15253) [M::mem_pestat] mean and std.dev: (3362.26, 2931.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20092) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 380.055 CPU sec, 41.601 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24686, 927651, 23111, 24376) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (697, 2448, 5548) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15250) [M::mem_pestat] mean and std.dev: (3331.11, 2937.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20101) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 140, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 332) [M::mem_pestat] mean and std.dev: (148.27, 53.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 406) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (810, 2636, 5695) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15465) [M::mem_pestat] mean and std.dev: (3442.98, 2930.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20350) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (740, 2497, 5577) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15251) [M::mem_pestat] mean and std.dev: (3357.65, 2928.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20088) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 378.197 CPU sec, 35.958 real sec [M::process] read 5555556 sequences (200000016 bp)... [M::process] read 5555556 sequences (200000016 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24376, 926423, 23585, 24246) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (717, 2482, 5567) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15267) [M::mem_pestat] mean and std.dev: (3343.23, 2934.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20117) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 140, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 332) [M::mem_pestat] mean and std.dev: (148.34, 53.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 406) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (821, 2625, 5725) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15533) [M::mem_pestat] mean and std.dev: (3449.30, 2947.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20437) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (771, 2604, 5637) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15369) [M::mem_pestat] mean and std.dev: (3412.60, 2940.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20235) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 356.579 CPU sec, 24.016 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24475, 924724, 23255, 24647) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (713, 2471, 5622) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15440) [M::mem_pestat] mean and std.dev: (3362.47, 2950.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20349) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 140, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 332) [M::mem_pestat] mean and std.dev: (148.43, 53.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 406) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (832, 2661, 5721) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15499) [M::mem_pestat] mean and std.dev: (3471.69, 2948.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20388) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (730, 2480, 5544) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15172) [M::mem_pestat] mean and std.dev: (3344.05, 2928.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19986) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 358.329 CPU sec, 27.967 real sec [M::process] read 5555556 sequences (200000016 bp)... [M::process] read 5555556 sequences (200000016 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24460, 925228, 23449, 24179) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (745, 2476, 5620) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15370) [M::mem_pestat] mean and std.dev: (3363.05, 2935.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20245) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 140, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 332) [M::mem_pestat] mean and std.dev: (148.48, 53.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 406) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (823, 2696, 5746) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15592) [M::mem_pestat] mean and std.dev: (3482.72, 2945.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20515) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (731, 2493, 5577) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15269) [M::mem_pestat] mean and std.dev: (3360.28, 2945.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20115) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 382.008 CPU sec, 41.056 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24511, 925358, 23422, 24288) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (704, 2452, 5611) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15425) [M::mem_pestat] mean and std.dev: (3342.26, 2947.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20332) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 140, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 332) [M::mem_pestat] mean and std.dev: (148.41, 53.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 406) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (828, 2694, 5747) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15585) [M::mem_pestat] mean and std.dev: (3478.54, 2943.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20504) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (746, 2462, 5639) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15425) [M::mem_pestat] mean and std.dev: (3369.45, 2942.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20318) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 381.818 CPU sec, 36.928 real sec [M::process] read 5555556 sequences (200000016 bp)... [M::process] read 5555556 sequences (200000016 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24117, 927244, 23205, 24319) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (726, 2477, 5612) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15384) [M::mem_pestat] mean and std.dev: (3358.33, 2947.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20270) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 140, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 332) [M::mem_pestat] mean and std.dev: (148.35, 53.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 406) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (823, 2580, 5729) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15541) [M::mem_pestat] mean and std.dev: (3441.82, 2943.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20447) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (732, 2480, 5548) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15180) [M::mem_pestat] mean and std.dev: (3344.14, 2923.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19996) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 376.954 CPU sec, 39.638 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24325, 927345, 23327, 24460) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (726, 2503, 5626) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15426) [M::mem_pestat] mean and std.dev: (3367.97, 2940.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20326) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 140, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 332) [M::mem_pestat] mean and std.dev: (148.36, 53.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 406) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (828, 2698, 5720) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15504) [M::mem_pestat] mean and std.dev: (3464.68, 2931.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20396) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (755, 2545, 5680) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15530) [M::mem_pestat] mean and std.dev: (3394.05, 2947.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20455) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 382.140 CPU sec, 37.501 real sec [M::process] read 5555556 sequences (200000016 bp)... [M::process] read 5555556 sequences (200000016 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24344, 926938, 23259, 24324) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (713, 2446, 5515) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15119) [M::mem_pestat] mean and std.dev: (3337.93, 2940.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19921) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 140, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 332) [M::mem_pestat] mean and std.dev: (148.38, 53.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 406) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (834, 2644, 5757) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15603) [M::mem_pestat] mean and std.dev: (3461.80, 2946.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20526) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (745, 2521, 5676) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15538) [M::mem_pestat] mean and std.dev: (3395.22, 2961.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20469) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 380.283 CPU sec, 40.822 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24648, 928766, 23128, 24244) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (710, 2443, 5542) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15206) [M::mem_pestat] mean and std.dev: (3320.58, 2932.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20038) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 140, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 332) [M::mem_pestat] mean and std.dev: (148.34, 53.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 406) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (845, 2653, 5719) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15467) [M::mem_pestat] mean and std.dev: (3463.77, 2937.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20341) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (754, 2544, 5583) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15241) [M::mem_pestat] mean and std.dev: (3380.01, 2932.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20070) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 378.891 CPU sec, 37.482 real sec [M::process] read 5555556 sequences (200000016 bp)... [M::process] read 5555556 sequences (200000016 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24535, 925506, 23633, 24419) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (720, 2483, 5559) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15237) [M::mem_pestat] mean and std.dev: (3342.91, 2930.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20076) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 140, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 332) [M::mem_pestat] mean and std.dev: (148.40, 53.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 406) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (789, 2611, 5662) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15408) [M::mem_pestat] mean and std.dev: (3434.79, 2943.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20281) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (743, 2511, 5599) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15311) [M::mem_pestat] mean and std.dev: (3375.36, 2939.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20167) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 358.431 CPU sec, 23.342 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24091, 935432, 23009, 23643) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (716, 2470, 5556) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15236) [M::mem_pestat] mean and std.dev: (3347.21, 2946.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20076) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 138, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 319) [M::mem_pestat] mean and std.dev: (145.26, 50.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 389) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (812, 2638, 5692) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15452) [M::mem_pestat] mean and std.dev: (3444.86, 2932.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20332) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (749, 2490, 5626) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15380) [M::mem_pestat] mean and std.dev: (3363.76, 2931.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20257) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 353.525 CPU sec, 24.870 real sec [M::process] read 5555556 sequences (200000016 bp)... [M::process] read 5555556 sequences (200000016 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23561, 935048, 22803, 23847) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (713, 2421, 5526) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15152) [M::mem_pestat] mean and std.dev: (3324.01, 2934.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19965) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 138, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 322) [M::mem_pestat] mean and std.dev: (145.53, 51.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 393) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (836, 2644, 5718) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15482) [M::mem_pestat] mean and std.dev: (3449.98, 2929.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20364) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (703, 2452, 5505) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15109) [M::mem_pestat] mean and std.dev: (3324.62, 2932.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19911) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 345.936 CPU sec, 22.914 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24190, 942181, 22757, 23818) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (697, 2430, 5590) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15376) [M::mem_pestat] mean and std.dev: (3332.69, 2950.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20269) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 138, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 319) [M::mem_pestat] mean and std.dev: (144.93, 50.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 389) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (825, 2637, 5773) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15669) [M::mem_pestat] mean and std.dev: (3461.53, 2942.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20617) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (733, 2520, 5646) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15472) [M::mem_pestat] mean and std.dev: (3381.46, 2949.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20385) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 347.842 CPU sec, 23.448 real sec [M::process] read 5555556 sequences (200000016 bp)... [M::process] read 5555556 sequences (200000016 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23838, 938495, 22858, 23876) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (705, 2473, 5544) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15222) [M::mem_pestat] mean and std.dev: (3335.19, 2931.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20061) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 138, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 319) [M::mem_pestat] mean and std.dev: (145.13, 50.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 389) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (804, 2597, 5669) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15399) [M::mem_pestat] mean and std.dev: (3428.70, 2937.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20264) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (735, 2492, 5526) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15108) [M::mem_pestat] mean and std.dev: (3339.23, 2920.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19899) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 348.955 CPU sec, 22.892 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24131, 930832, 22896, 23964) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (709, 2483, 5588) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15346) [M::mem_pestat] mean and std.dev: (3343.25, 2932.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20225) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 138, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 322) [M::mem_pestat] mean and std.dev: (145.71, 51.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 393) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (804, 2608, 5712) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15528) [M::mem_pestat] mean and std.dev: (3425.66, 2932.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20436) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (738, 2520, 5553) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15183) [M::mem_pestat] mean and std.dev: (3367.73, 2928.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19998) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 352.356 CPU sec, 24.729 real sec [M::process] read 5555556 sequences (200000016 bp)... [M::process] read 5555556 sequences (200000016 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23951, 937951, 23062, 23660) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (707, 2461, 5521) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15149) [M::mem_pestat] mean and std.dev: (3320.89, 2924.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19963) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 138, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 322) [M::mem_pestat] mean and std.dev: (145.43, 51.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 393) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (826, 2617, 5741) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15571) [M::mem_pestat] mean and std.dev: (3454.68, 2951.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20486) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (732, 2492, 5573) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15255) [M::mem_pestat] mean and std.dev: (3355.07, 2938.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20096) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 376.621 CPU sec, 41.161 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24097, 943267, 22565, 23525) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (702, 2447, 5524) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15168) [M::mem_pestat] mean and std.dev: (3319.39, 2926.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19990) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 138, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 319) [M::mem_pestat] mean and std.dev: (145.01, 50.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 389) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (811, 2635, 5695) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15463) [M::mem_pestat] mean and std.dev: (3437.93, 2932.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20347) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (725, 2467, 5548) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15194) [M::mem_pestat] mean and std.dev: (3334.58, 2927.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20017) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 377.765 CPU sec, 35.853 real sec [M::process] read 5555556 sequences (200000016 bp)... [M::process] read 5555556 sequences (200000016 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23939, 932254, 22663, 23780) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (718, 2443, 5586) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15322) [M::mem_pestat] mean and std.dev: (3335.32, 2934.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20190) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 138, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 322) [M::mem_pestat] mean and std.dev: (145.52, 50.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 393) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (829, 2670, 5714) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15484) [M::mem_pestat] mean and std.dev: (3453.94, 2920.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20369) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (737, 2487, 5585) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15281) [M::mem_pestat] mean and std.dev: (3349.90, 2923.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20129) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 378.234 CPU sec, 41.056 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23985, 935198, 22944, 23825) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (721, 2515, 5608) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15382) [M::mem_pestat] mean and std.dev: (3358.01, 2942.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20269) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 138, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 322) [M::mem_pestat] mean and std.dev: (145.53, 51.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 393) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (812, 2658, 5691) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15449) [M::mem_pestat] mean and std.dev: (3453.32, 2939.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20328) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (748, 2520, 5645) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15439) [M::mem_pestat] mean and std.dev: (3384.87, 2942.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20336) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 381.778 CPU sec, 38.198 real sec [M::process] read 5555556 sequences (200000016 bp)... [M::process] read 5555556 sequences (200000016 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23963, 944085, 22740, 23732) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (694, 2388, 5491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15085) [M::mem_pestat] mean and std.dev: (3297.24, 2927.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19882) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 138, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 319) [M::mem_pestat] mean and std.dev: (144.98, 50.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 389) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (800, 2633, 5766) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15698) [M::mem_pestat] mean and std.dev: (3455.49, 2954.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20664) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (713, 2467, 5509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15101) [M::mem_pestat] mean and std.dev: (3321.79, 2925.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19897) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 355.881 CPU sec, 23.319 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23587, 936131, 22638, 23716) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (695, 2433, 5503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15119) [M::mem_pestat] mean and std.dev: (3308.54, 2928.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19927) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 138, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 319) [M::mem_pestat] mean and std.dev: (144.99, 50.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 389) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (834, 2664, 5705) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15447) [M::mem_pestat] mean and std.dev: (3447.95, 2914.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20318) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (742, 2526, 5654) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15478) [M::mem_pestat] mean and std.dev: (3380.06, 2941.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20390) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 350.890 CPU sec, 23.759 real sec [M::process] read 5555556 sequences (200000016 bp)... [M::process] read 5555556 sequences (200000016 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24141, 936917, 22760, 23918) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (698, 2418, 5539) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15221) [M::mem_pestat] mean and std.dev: (3320.67, 2943.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20062) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 138, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 319) [M::mem_pestat] mean and std.dev: (145.20, 50.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 389) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (816, 2630, 5690) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15438) [M::mem_pestat] mean and std.dev: (3444.24, 2933.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20312) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (730, 2442, 5562) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15226) [M::mem_pestat] mean and std.dev: (3331.16, 2925.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20058) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 379.986 CPU sec, 40.813 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24112, 943112, 22967, 24039) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (691, 2430, 5537) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15229) [M::mem_pestat] mean and std.dev: (3316.48, 2937.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20075) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 138, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 319) [M::mem_pestat] mean and std.dev: (144.93, 50.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 389) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (813, 2684, 5720) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15534) [M::mem_pestat] mean and std.dev: (3461.53, 2943.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20441) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (742, 2517, 5664) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15508) [M::mem_pestat] mean and std.dev: (3385.50, 2952.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20430) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 382.753 CPU sec, 38.437 real sec [M::process] read 5555556 sequences (200000016 bp)... [M::process] read 5555556 sequences (200000016 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23886, 943483, 22794, 23714) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (732, 2508, 5558) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15210) [M::mem_pestat] mean and std.dev: (3348.02, 2924.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20036) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 138, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 319) [M::mem_pestat] mean and std.dev: (145.02, 50.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 389) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (812, 2636, 5729) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15563) [M::mem_pestat] mean and std.dev: (3450.91, 2936.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20480) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (745, 2519, 5578) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15244) [M::mem_pestat] mean and std.dev: (3368.49, 2934.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20077) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 374.823 CPU sec, 40.828 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24049, 934129, 22620, 23503) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (706, 2463, 5610) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15418) [M::mem_pestat] mean and std.dev: (3353.30, 2952.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20322) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 138, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 322) [M::mem_pestat] mean and std.dev: (145.57, 51.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 393) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (827, 2689, 5803) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15755) [M::mem_pestat] mean and std.dev: (3491.91, 2963.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20731) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (749, 2526, 5644) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15434) [M::mem_pestat] mean and std.dev: (3372.48, 2930.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20329) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 373.886 CPU sec, 34.936 real sec [M::process] read 5555556 sequences (200000016 bp)... [M::process] read 5555556 sequences (200000016 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23860, 939639, 22861, 23696) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (699, 2516, 5586) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15360) [M::mem_pestat] mean and std.dev: (3354.92, 2944.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20247) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 138, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 319) [M::mem_pestat] mean and std.dev: (145.10, 50.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 389) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (811, 2667, 5761) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15661) [M::mem_pestat] mean and std.dev: (3474.85, 2951.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20611) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (725, 2482, 5575) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15275) [M::mem_pestat] mean and std.dev: (3347.38, 2937.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20125) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 351.120 CPU sec, 23.635 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23794, 948478, 22691, 23695) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (686, 2390, 5504) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15140) [M::mem_pestat] mean and std.dev: (3297.39, 2925.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19958) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 137, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 316) [M::mem_pestat] mean and std.dev: (144.54, 50.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 385) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (819, 2669, 5788) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15726) [M::mem_pestat] mean and std.dev: (3477.71, 2949.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20695) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (739, 2521, 5663) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15511) [M::mem_pestat] mean and std.dev: (3387.16, 2942.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20435) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 357.384 CPU sec, 25.515 real sec [M::process] read 5555556 sequences (200000016 bp)... [M::process] read 5555556 sequences (200000016 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23720, 934372, 22693, 23590) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (701, 2419, 5541) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15221) [M::mem_pestat] mean and std.dev: (3314.60, 2935.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20061) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 138, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 319) [M::mem_pestat] mean and std.dev: (145.03, 50.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 389) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (802, 2604, 5670) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15406) [M::mem_pestat] mean and std.dev: (3431.51, 2946.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20274) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (729, 2464, 5562) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15228) [M::mem_pestat] mean and std.dev: (3341.74, 2932.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20061) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 353.526 CPU sec, 23.278 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24120, 940046, 22700, 23852) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (722, 2484, 5608) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15380) [M::mem_pestat] mean and std.dev: (3361.19, 2953.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20266) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 138, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 319) [M::mem_pestat] mean and std.dev: (145.07, 50.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 389) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (814, 2645, 5704) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15484) [M::mem_pestat] mean and std.dev: (3454.93, 2941.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20374) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (733, 2492, 5627) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15415) [M::mem_pestat] mean and std.dev: (3368.89, 2947.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20309) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 353.195 CPU sec, 24.540 real sec [M::process] read 5555556 sequences (200000016 bp)... [M::process] read 5555556 sequences (200000016 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24023, 947084, 22592, 23537) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (719, 2487, 5540) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15182) [M::mem_pestat] mean and std.dev: (3351.15, 2939.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20003) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 137, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 316) [M::mem_pestat] mean and std.dev: (144.60, 50.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 385) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (817, 2684, 5736) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15574) [M::mem_pestat] mean and std.dev: (3466.61, 2942.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20493) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (723, 2445, 5534) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15156) [M::mem_pestat] mean and std.dev: (3326.10, 2926.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19967) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 376.515 CPU sec, 42.847 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23861, 934022, 22730, 23909) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (712, 2439, 5509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15103) [M::mem_pestat] mean and std.dev: (3325.46, 2931.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19900) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 138, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 322) [M::mem_pestat] mean and std.dev: (145.49, 51.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 393) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (822, 2619, 5676) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15384) [M::mem_pestat] mean and std.dev: (3444.30, 2941.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20238) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (755, 2507, 5589) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15257) [M::mem_pestat] mean and std.dev: (3365.86, 2923.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20091) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 379.162 CPU sec, 37.459 real sec [M::process] read 5555556 sequences (200000016 bp)... [M::process] read 5555556 sequences (200000016 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24077, 934566, 22766, 23742) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (743, 2520, 5665) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15509) [M::mem_pestat] mean and std.dev: (3380.77, 2942.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20431) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 138, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 322) [M::mem_pestat] mean and std.dev: (145.53, 51.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 393) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (799, 2650, 5740) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15622) [M::mem_pestat] mean and std.dev: (3449.86, 2945.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20563) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (741, 2489, 5609) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15345) [M::mem_pestat] mean and std.dev: (3364.85, 2934.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20213) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 355.463 CPU sec, 23.441 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23798, 941381, 22866, 23914) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (710, 2422, 5504) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15092) [M::mem_pestat] mean and std.dev: (3314.98, 2927.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19886) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 137, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 319) [M::mem_pestat] mean and std.dev: (144.84, 50.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 389) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (796, 2640, 5705) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15523) [M::mem_pestat] mean and std.dev: (3442.73, 2945.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20432) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (744, 2466, 5616) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15360) [M::mem_pestat] mean and std.dev: (3372.09, 2947.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20232) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 353.579 CPU sec, 23.982 real sec [M::process] read 5555556 sequences (200000016 bp)... [M::process] read 5555556 sequences (200000016 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24165, 936700, 23000, 23868) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (726, 2456, 5594) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15330) [M::mem_pestat] mean and std.dev: (3354.70, 2948.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20198) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 138, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 322) [M::mem_pestat] mean and std.dev: (145.67, 51.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 393) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (823, 2668, 5732) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15550) [M::mem_pestat] mean and std.dev: (3470.20, 2951.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20459) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (736, 2472, 5591) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15301) [M::mem_pestat] mean and std.dev: (3354.55, 2933.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20156) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 374.963 CPU sec, 40.214 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23877, 933406, 22839, 24109) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (714, 2435, 5558) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15246) [M::mem_pestat] mean and std.dev: (3335.41, 2944.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20090) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 138, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 322) [M::mem_pestat] mean and std.dev: (145.90, 51.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 393) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (825, 2650, 5732) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15546) [M::mem_pestat] mean and std.dev: (3465.72, 2950.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20453) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (738, 2502, 5564) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15216) [M::mem_pestat] mean and std.dev: (3358.28, 2933.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20042) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 372.293 CPU sec, 34.877 real sec [M::process] read 5555556 sequences (200000016 bp)... [M::process] read 5555556 sequences (200000016 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23932, 937235, 22798, 23765) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (729, 2482, 5570) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15252) [M::mem_pestat] mean and std.dev: (3355.57, 2946.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20093) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 138, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 319) [M::mem_pestat] mean and std.dev: (145.23, 50.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 389) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (827, 2646, 5749) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15593) [M::mem_pestat] mean and std.dev: (3466.68, 2952.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20515) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (749, 2546, 5656) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15470) [M::mem_pestat] mean and std.dev: (3396.42, 2938.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20377) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 354.556 CPU sec, 23.502 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24098, 941997, 22733, 23840) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (699, 2483, 5614) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15444) [M::mem_pestat] mean and std.dev: (3357.24, 2951.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20359) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 138, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 319) [M::mem_pestat] mean and std.dev: (145.16, 50.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 389) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (805, 2625, 5681) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15433) [M::mem_pestat] mean and std.dev: (3429.68, 2932.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20309) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (732, 2466, 5511) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15069) [M::mem_pestat] mean and std.dev: (3321.72, 2909.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19848) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 352.646 CPU sec, 24.586 real sec [M::process] read 5555556 sequences (200000016 bp)... [M::process] read 5555556 sequences (200000016 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23784, 934256, 22980, 23761) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (734, 2499, 5603) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15341) [M::mem_pestat] mean and std.dev: (3368.90, 2945.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20210) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 138, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 322) [M::mem_pestat] mean and std.dev: (145.62, 51.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 393) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (828, 2670, 5738) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15558) [M::mem_pestat] mean and std.dev: (3466.08, 2943.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20468) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (751, 2503, 5547) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15139) [M::mem_pestat] mean and std.dev: (3358.87, 2927.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19935) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 362.832 CPU sec, 35.312 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23589, 935386, 23138, 23697) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (691, 2439, 5489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15085) [M::mem_pestat] mean and std.dev: (3306.16, 2919.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19883) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 138, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 322) [M::mem_pestat] mean and std.dev: (145.55, 51.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 393) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (811, 2658, 5656) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15346) [M::mem_pestat] mean and std.dev: (3440.67, 2929.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20191) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (732, 2469, 5590) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15306) [M::mem_pestat] mean and std.dev: (3348.31, 2934.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20164) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 365.687 CPU sec, 32.361 real sec [M::process] read 5555556 sequences (200000016 bp)... [M::process] read 5555556 sequences (200000016 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23875, 943819, 22921, 23937) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (716, 2409, 5516) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15116) [M::mem_pestat] mean and std.dev: (3315.12, 2927.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19916) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 138, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 319) [M::mem_pestat] mean and std.dev: (144.95, 50.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 389) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (815, 2670, 5769) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15677) [M::mem_pestat] mean and std.dev: (3470.66, 2951.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20631) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (755, 2512, 5592) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15266) [M::mem_pestat] mean and std.dev: (3366.87, 2927.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20103) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 348.581 CPU sec, 23.031 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23850, 937998, 22899, 23826) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (711, 2442, 5583) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15327) [M::mem_pestat] mean and std.dev: (3340.29, 2948.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20199) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 138, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 319) [M::mem_pestat] mean and std.dev: (145.16, 50.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 389) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (825, 2664, 5751) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15603) [M::mem_pestat] mean and std.dev: (3469.27, 2957.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20529) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (734, 2496, 5566) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15230) [M::mem_pestat] mean and std.dev: (3358.76, 2932.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20062) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 359.887 CPU sec, 26.974 real sec [M::process] read 5555556 sequences (200000016 bp)... [M::process] read 5555556 sequences (200000016 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23817, 937755, 22938, 23701) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (732, 2523, 5599) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15333) [M::mem_pestat] mean and std.dev: (3374.06, 2940.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20200) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 138, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 319) [M::mem_pestat] mean and std.dev: (145.20, 50.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 389) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (812, 2659, 5707) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15497) [M::mem_pestat] mean and std.dev: (3453.81, 2940.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20392) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (734, 2519, 5635) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15437) [M::mem_pestat] mean and std.dev: (3381.02, 2946.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20338) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 374.749 CPU sec, 40.203 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23736, 947204, 22789, 23671) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (729, 2467, 5540) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15162) [M::mem_pestat] mean and std.dev: (3342.77, 2930.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19973) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 137, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 316) [M::mem_pestat] mean and std.dev: (144.46, 50.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 385) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (837, 2742, 5822) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15792) [M::mem_pestat] mean and std.dev: (3512.50, 2957.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20777) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (733, 2495, 5630) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15424) [M::mem_pestat] mean and std.dev: (3367.23, 2939.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20321) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 378.156 CPU sec, 36.147 real sec [M::process] read 5555556 sequences (200000016 bp)... [M::process] read 5555556 sequences (200000016 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23999, 936634, 22997, 23786) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (704, 2421, 5560) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15272) [M::mem_pestat] mean and std.dev: (3326.93, 2946.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20128) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 138, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 322) [M::mem_pestat] mean and std.dev: (145.53, 51.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 393) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (825, 2658, 5794) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15732) [M::mem_pestat] mean and std.dev: (3475.36, 2946.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20701) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (731, 2517, 5566) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15236) [M::mem_pestat] mean and std.dev: (3359.49, 2932.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20071) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 376.272 CPU sec, 41.746 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24024, 935178, 23142, 23896) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (711, 2475, 5538) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15192) [M::mem_pestat] mean and std.dev: (3328.75, 2922.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20019) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 138, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 322) [M::mem_pestat] mean and std.dev: (145.69, 51.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 393) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (795, 2649, 5744) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15642) [M::mem_pestat] mean and std.dev: (3455.13, 2960.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20591) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (746, 2553, 5627) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15389) [M::mem_pestat] mean and std.dev: (3380.18, 2939.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20270) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 377.123 CPU sec, 36.735 real sec [M::process] read 5555556 sequences (200000016 bp)... [M::process] read 5555556 sequences (200000016 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24126, 943680, 22444, 23708) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (711, 2396, 5581) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15321) [M::mem_pestat] mean and std.dev: (3329.03, 2946.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20191) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 137, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 319) [M::mem_pestat] mean and std.dev: (144.94, 50.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 389) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (846, 2642, 5765) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15603) [M::mem_pestat] mean and std.dev: (3472.12, 2948.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20522) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (746, 2523, 5637) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15419) [M::mem_pestat] mean and std.dev: (3381.86, 2939.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20310) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 349.818 CPU sec, 23.123 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23908, 945228, 22972, 23912) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (722, 2479, 5581) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15299) [M::mem_pestat] mean and std.dev: (3345.41, 2937.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20158) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 138, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 319) [M::mem_pestat] mean and std.dev: (145.03, 50.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 389) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (834, 2693, 5776) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15660) [M::mem_pestat] mean and std.dev: (3482.40, 2944.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20602) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (762, 2546, 5631) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15369) [M::mem_pestat] mean and std.dev: (3395.24, 2939.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20238) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 352.648 CPU sec, 24.382 real sec [M::process] read 5555556 sequences (200000016 bp)... [M::process] read 5555556 sequences (200000016 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23947, 936363, 22820, 23822) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (715, 2475, 5507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15091) [M::mem_pestat] mean and std.dev: (3331.22, 2930.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19883) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 138, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 319) [M::mem_pestat] mean and std.dev: (145.19, 50.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 389) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (818, 2654, 5730) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15554) [M::mem_pestat] mean and std.dev: (3458.75, 2944.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20466) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (749, 2466, 5542) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15128) [M::mem_pestat] mean and std.dev: (3346.07, 2921.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19921) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 373.423 CPU sec, 38.818 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23964, 938885, 22957, 23593) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (714, 2455, 5547) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15213) [M::mem_pestat] mean and std.dev: (3334.37, 2936.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20046) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 138, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 319) [M::mem_pestat] mean and std.dev: (144.99, 50.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 389) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (835, 2639, 5660) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15310) [M::mem_pestat] mean and std.dev: (3441.41, 2924.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20135) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (764, 2501, 5567) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15173) [M::mem_pestat] mean and std.dev: (3369.17, 2931.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19976) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 377.126 CPU sec, 36.479 real sec [M::process] read 5555556 sequences (200000016 bp)... [M::process] read 5555556 sequences (200000016 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24096, 945061, 22748, 23533) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (719, 2461, 5540) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15182) [M::mem_pestat] mean and std.dev: (3328.78, 2929.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20003) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 137, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 316) [M::mem_pestat] mean and std.dev: (144.59, 50.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 385) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (810, 2657, 5638) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15294) [M::mem_pestat] mean and std.dev: (3434.81, 2929.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20122) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (718, 2487, 5542) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15190) [M::mem_pestat] mean and std.dev: (3341.07, 2930.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20014) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 376.731 CPU sec, 41.063 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23736, 942386, 22854, 23518) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (732, 2497, 5588) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15300) [M::mem_pestat] mean and std.dev: (3356.85, 2940.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20156) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 138, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 319) [M::mem_pestat] mean and std.dev: (145.10, 50.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 389) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (839, 2591, 5655) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15287) [M::mem_pestat] mean and std.dev: (3430.48, 2922.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20103) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (714, 2472, 5526) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15150) [M::mem_pestat] mean and std.dev: (3332.33, 2931.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19962) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 378.163 CPU sec, 37.592 real sec [M::process] read 5555556 sequences (200000016 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24077, 938396, 22924, 23660) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (691, 2423, 5568) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15322) [M::mem_pestat] mean and std.dev: (3328.18, 2948.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20199) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 138, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 322) [M::mem_pestat] mean and std.dev: (145.65, 51.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 393) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (811, 2635, 5705) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15493) [M::mem_pestat] mean and std.dev: (3444.47, 2939.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20387) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (741, 2511, 5566) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15216) [M::mem_pestat] mean and std.dev: (3357.38, 2924.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20041) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 350.095 CPU sec, 24.681 real sec [M::process] read 5555556 sequences (200000016 bp)... [M::process] read 5555556 sequences (200000016 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24070, 940840, 22891, 24038) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (712, 2465, 5519) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15133) [M::mem_pestat] mean and std.dev: (3319.24, 2924.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19940) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 138, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 319) [M::mem_pestat] mean and std.dev: (145.12, 50.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 389) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (815, 2652, 5751) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15623) [M::mem_pestat] mean and std.dev: (3459.03, 2942.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20559) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (740, 2520, 5483) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14969) [M::mem_pestat] mean and std.dev: (3348.74, 2914.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19712) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 351.171 CPU sec, 23.106 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23722, 943931, 22774, 23748) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (706, 2469, 5575) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15313) [M::mem_pestat] mean and std.dev: (3331.97, 2929.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20182) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 138, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 319) [M::mem_pestat] mean and std.dev: (144.91, 50.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 389) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (816, 2633, 5700) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15468) [M::mem_pestat] mean and std.dev: (3445.49, 2927.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20352) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (735, 2470, 5565) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15225) [M::mem_pestat] mean and std.dev: (3340.02, 2924.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20055) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 357.402 CPU sec, 25.876 real sec [M::process] read 5555556 sequences (200000016 bp)... [M::process] read 5555556 sequences (200000016 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24160, 935502, 23111, 23867) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (712, 2475, 5584) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15328) [M::mem_pestat] mean and std.dev: (3344.34, 2940.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20200) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 138, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 322) [M::mem_pestat] mean and std.dev: (145.75, 51.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 393) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (810, 2671, 5790) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15750) [M::mem_pestat] mean and std.dev: (3476.43, 2950.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20730) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (746, 2496, 5572) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15224) [M::mem_pestat] mean and std.dev: (3364.83, 2937.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20050) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 343.851 CPU sec, 22.639 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24131, 942713, 22687, 23732) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (704, 2445, 5534) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15194) [M::mem_pestat] mean and std.dev: (3330.66, 2943.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20024) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 138, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 319) [M::mem_pestat] mean and std.dev: (145.13, 50.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 389) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (823, 2662, 5650) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15304) [M::mem_pestat] mean and std.dev: (3436.20, 2924.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20131) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (751, 2533, 5611) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15331) [M::mem_pestat] mean and std.dev: (3383.42, 2934.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20191) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 350.912 CPU sec, 23.809 real sec [M::process] read 5555556 sequences (200000016 bp)... [M::process] read 5555556 sequences (200000016 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24036, 941100, 22726, 23854) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (716, 2477, 5608) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15392) [M::mem_pestat] mean and std.dev: (3350.09, 2946.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20284) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 138, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 319) [M::mem_pestat] mean and std.dev: (145.15, 50.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 389) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (834, 2699, 5720) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15492) [M::mem_pestat] mean and std.dev: (3468.17, 2937.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20378) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (732, 2488, 5557) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15207) [M::mem_pestat] mean and std.dev: (3347.34, 2924.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20032) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 376.819 CPU sec, 41.988 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23782, 940588, 22443, 23532) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (722, 2488, 5635) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15461) [M::mem_pestat] mean and std.dev: (3360.45, 2946.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20374) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 138, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 319) [M::mem_pestat] mean and std.dev: (145.14, 50.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 389) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (802, 2628, 5683) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15445) [M::mem_pestat] mean and std.dev: (3441.15, 2938.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20326) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (728, 2464, 5583) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15293) [M::mem_pestat] mean and std.dev: (3349.22, 2935.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20148) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 376.469 CPU sec, 36.485 real sec [M::process] read 5555556 sequences (200000016 bp)... [M::process] read 5555556 sequences (200000016 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24026, 939854, 23003, 23918) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (693, 2401, 5551) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15267) [M::mem_pestat] mean and std.dev: (3316.20, 2939.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20125) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 138, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 319) [M::mem_pestat] mean and std.dev: (145.26, 50.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 389) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (814, 2600, 5642) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15298) [M::mem_pestat] mean and std.dev: (3416.00, 2924.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20126) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (739, 2511, 5574) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15244) [M::mem_pestat] mean and std.dev: (3363.06, 2933.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20079) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 350.236 CPU sec, 22.978 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23943, 939343, 23012, 23764) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (690, 2498, 5600) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15420) [M::mem_pestat] mean and std.dev: (3350.34, 2946.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20330) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 138, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 319) [M::mem_pestat] mean and std.dev: (144.99, 50.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 389) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (798, 2614, 5716) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15552) [M::mem_pestat] mean and std.dev: (3441.06, 2942.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20470) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (741, 2480, 5622) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15384) [M::mem_pestat] mean and std.dev: (3372.99, 2943.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20265) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 350.186 CPU sec, 23.813 real sec [M::process] read 5555556 sequences (200000016 bp)... [M::process] read 5555556 sequences (200000016 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23888, 944526, 22888, 23599) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (726, 2502, 5563) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15237) [M::mem_pestat] mean and std.dev: (3346.77, 2932.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20074) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 137, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 319) [M::mem_pestat] mean and std.dev: (144.78, 50.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 389) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (809, 2612, 5646) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15320) [M::mem_pestat] mean and std.dev: (3427.67, 2922.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20157) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (740, 2549, 5686) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15578) [M::mem_pestat] mean and std.dev: (3405.43, 2959.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20524) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 372.731 CPU sec, 37.597 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24002, 942036, 22779, 23789) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (699, 2454, 5570) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15312) [M::mem_pestat] mean and std.dev: (3339.83, 2948.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20183) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 138, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 319) [M::mem_pestat] mean and std.dev: (145.17, 50.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 389) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (830, 2649, 5681) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15383) [M::mem_pestat] mean and std.dev: (3452.05, 2937.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20234) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (759, 2522, 5614) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15324) [M::mem_pestat] mean and std.dev: (3377.82, 2923.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20179) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 371.832 CPU sec, 33.961 real sec [M::process] read 5555556 sequences (200000016 bp)... [M::process] read 5555556 sequences (200000016 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24005, 941961, 22840, 23386) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (721, 2452, 5581) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15301) [M::mem_pestat] mean and std.dev: (3339.53, 2941.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20161) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 137, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 319) [M::mem_pestat] mean and std.dev: (144.89, 50.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 389) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (798, 2609, 5651) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15357) [M::mem_pestat] mean and std.dev: (3425.67, 2935.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20210) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (736, 2504, 5604) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15340) [M::mem_pestat] mean and std.dev: (3359.68, 2935.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20208) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 380.215 CPU sec, 42.694 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23722, 947122, 22681, 23543) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (702, 2429, 5596) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15384) [M::mem_pestat] mean and std.dev: (3334.80, 2947.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20278) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (108, 137, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 318) [M::mem_pestat] mean and std.dev: (144.56, 50.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 388) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (815, 2641, 5714) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15512) [M::mem_pestat] mean and std.dev: (3445.94, 2941.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20411) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (750, 2519, 5588) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15264) [M::mem_pestat] mean and std.dev: (3378.48, 2942.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20102) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 376.542 CPU sec, 36.351 real sec [M::process] read 5555556 sequences (200000016 bp)... [M::process] read 5555556 sequences (200000016 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23905, 937576, 22994, 23787) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (722, 2485, 5565) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15251) [M::mem_pestat] mean and std.dev: (3351.44, 2938.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20094) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 138, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 322) [M::mem_pestat] mean and std.dev: (145.45, 51.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 393) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (821, 2680, 5784) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15710) [M::mem_pestat] mean and std.dev: (3487.43, 2959.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20673) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (728, 2483, 5639) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15461) [M::mem_pestat] mean and std.dev: (3374.39, 2958.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20372) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 351.788 CPU sec, 23.318 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24044, 945554, 22665, 23554) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (712, 2450, 5547) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15217) [M::mem_pestat] mean and std.dev: (3332.69, 2936.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20052) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 138, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 319) [M::mem_pestat] mean and std.dev: (144.95, 50.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 389) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (819, 2608, 5770) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15672) [M::mem_pestat] mean and std.dev: (3450.60, 2953.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20623) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (733, 2461, 5626) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15412) [M::mem_pestat] mean and std.dev: (3357.91, 2943.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20305) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 351.941 CPU sec, 24.642 real sec [M::process] read 5555556 sequences (200000016 bp)... [M::process] read 5555556 sequences (200000016 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23794, 942530, 22903, 23731) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (690, 2412, 5531) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15213) [M::mem_pestat] mean and std.dev: (3308.94, 2936.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20054) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 137, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 316) [M::mem_pestat] mean and std.dev: (144.61, 50.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 385) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (807, 2661, 5769) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15693) [M::mem_pestat] mean and std.dev: (3472.29, 2957.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20655) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (745, 2549, 5693) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15589) [M::mem_pestat] mean and std.dev: (3397.98, 2944.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20537) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 346.713 CPU sec, 22.924 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24026, 939709, 22913, 23834) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (685, 2396, 5511) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15163) [M::mem_pestat] mean and std.dev: (3317.26, 2956.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19989) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 138, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 319) [M::mem_pestat] mean and std.dev: (145.00, 50.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 389) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (835, 2684, 5800) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15730) [M::mem_pestat] mean and std.dev: (3475.33, 2939.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20695) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (732, 2459, 5584) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15288) [M::mem_pestat] mean and std.dev: (3354.60, 2941.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20140) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 353.375 CPU sec, 24.517 real sec [M::process] read 5555556 sequences (200000016 bp)... [M::process] read 5555556 sequences (200000016 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23736, 941680, 23133, 23714) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (702, 2512, 5630) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15486) [M::mem_pestat] mean and std.dev: (3375.61, 2965.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20414) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 137, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 319) [M::mem_pestat] mean and std.dev: (144.85, 50.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 389) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (832, 2678, 5738) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15550) [M::mem_pestat] mean and std.dev: (3473.94, 2939.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20456) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (750, 2499, 5562) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15186) [M::mem_pestat] mean and std.dev: (3356.23, 2918.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19998) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 374.300 CPU sec, 40.782 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23763, 939777, 22787, 23716) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (706, 2464, 5552) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15244) [M::mem_pestat] mean and std.dev: (3333.15, 2931.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20090) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 137, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 319) [M::mem_pestat] mean and std.dev: (144.75, 50.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 389) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (828, 2628, 5665) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15339) [M::mem_pestat] mean and std.dev: (3441.62, 2933.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20176) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (748, 2540, 5632) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15400) [M::mem_pestat] mean and std.dev: (3387.49, 2939.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20284) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 368.873 CPU sec, 34.866 real sec [M::process] read 5555556 sequences (200000016 bp)... [M::process] read 5555556 sequences (200000016 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23853, 944900, 23049, 23843) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (723, 2490, 5557) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15225) [M::mem_pestat] mean and std.dev: (3345.98, 2939.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20059) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 138, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 319) [M::mem_pestat] mean and std.dev: (145.12, 50.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 389) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (814, 2647, 5685) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15427) [M::mem_pestat] mean and std.dev: (3450.36, 2937.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20298) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (744, 2496, 5589) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15279) [M::mem_pestat] mean and std.dev: (3363.91, 2939.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20124) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 374.408 CPU sec, 41.387 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23961, 936405, 22992, 23785) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (709, 2425, 5470) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14992) [M::mem_pestat] mean and std.dev: (3304.05, 2918.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19753) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 138, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 322) [M::mem_pestat] mean and std.dev: (145.70, 51.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 393) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (851, 2678, 5719) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15455) [M::mem_pestat] mean and std.dev: (3472.39, 2931.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20323) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (742, 2479, 5584) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15268) [M::mem_pestat] mean and std.dev: (3360.46, 2933.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20110) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 368.461 CPU sec, 35.216 real sec [M::process] read 5555556 sequences (200000016 bp)... [M::process] read 5555556 sequences (200000016 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23895, 940686, 22959, 23732) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (715, 2441, 5544) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15202) [M::mem_pestat] mean and std.dev: (3332.19, 2932.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20031) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 138, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 319) [M::mem_pestat] mean and std.dev: (145.16, 50.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 389) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (799, 2627, 5711) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15535) [M::mem_pestat] mean and std.dev: (3440.39, 2936.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20447) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (731, 2468, 5557) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15209) [M::mem_pestat] mean and std.dev: (3345.21, 2927.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20035) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 344.698 CPU sec, 22.993 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23958, 941977, 23016, 23896) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (703, 2465, 5539) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15211) [M::mem_pestat] mean and std.dev: (3331.55, 2932.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20047) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 138, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 319) [M::mem_pestat] mean and std.dev: (145.11, 50.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 389) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (825, 2691, 5732) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15546) [M::mem_pestat] mean and std.dev: (3467.25, 2944.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20453) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (739, 2443, 5552) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15178) [M::mem_pestat] mean and std.dev: (3335.32, 2921.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19991) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 349.067 CPU sec, 24.698 real sec [M::process] read 5555556 sequences (200000016 bp)... [M::process] read 5555556 sequences (200000016 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23898, 939222, 22969, 23949) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (713, 2469, 5577) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15305) [M::mem_pestat] mean and std.dev: (3351.31, 2945.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20169) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 138, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 322) [M::mem_pestat] mean and std.dev: (145.64, 51.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 393) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (807, 2691, 5760) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15666) [M::mem_pestat] mean and std.dev: (3477.77, 2957.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20619) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (739, 2492, 5582) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15268) [M::mem_pestat] mean and std.dev: (3367.26, 2942.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20111) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 377.047 CPU sec, 40.273 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23883, 938156, 22520, 23649) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (708, 2427, 5517) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15135) [M::mem_pestat] mean and std.dev: (3314.68, 2925.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19944) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 138, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 319) [M::mem_pestat] mean and std.dev: (145.29, 50.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 389) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (809, 2618, 5671) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15395) [M::mem_pestat] mean and std.dev: (3431.67, 2933.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20257) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (759, 2494, 5558) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15156) [M::mem_pestat] mean and std.dev: (3357.99, 2930.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19955) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 379.836 CPU sec, 39.116 real sec [M::process] read 5555556 sequences (200000016 bp)... [M::process] read 5555556 sequences (200000016 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23855, 939841, 22670, 23784) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (725, 2498, 5618) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15404) [M::mem_pestat] mean and std.dev: (3362.24, 2946.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20297) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 138, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 319) [M::mem_pestat] mean and std.dev: (145.18, 50.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 389) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (816, 2600, 5720) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15528) [M::mem_pestat] mean and std.dev: (3442.28, 2946.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20432) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (719, 2465, 5560) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15242) [M::mem_pestat] mean and std.dev: (3348.76, 2942.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20083) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 371.683 CPU sec, 39.186 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23607, 941550, 22719, 23940) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (718, 2455, 5492) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15040) [M::mem_pestat] mean and std.dev: (3322.23, 2920.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19814) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 138, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 319) [M::mem_pestat] mean and std.dev: (145.25, 50.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 389) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (824, 2670, 5729) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15539) [M::mem_pestat] mean and std.dev: (3456.42, 2935.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20444) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (762, 2533, 5526) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15054) [M::mem_pestat] mean and std.dev: (3359.66, 2911.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19818) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 371.328 CPU sec, 35.213 real sec [M::process] read 5555556 sequences (200000016 bp)... [M::process] read 5555556 sequences (200000016 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24244, 937163, 22886, 23597) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (729, 2475, 5548) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15186) [M::mem_pestat] mean and std.dev: (3339.59, 2927.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20005) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 138, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 322) [M::mem_pestat] mean and std.dev: (145.42, 51.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 393) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (835, 2702, 5746) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15568) [M::mem_pestat] mean and std.dev: (3468.54, 2933.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20479) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (725, 2497, 5526) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15128) [M::mem_pestat] mean and std.dev: (3343.00, 2919.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19929) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 376.449 CPU sec, 41.732 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23646, 941022, 22820, 23837) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (708, 2454, 5549) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15231) [M::mem_pestat] mean and std.dev: (3329.64, 2926.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20072) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 138, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 319) [M::mem_pestat] mean and std.dev: (145.03, 50.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 389) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (832, 2602, 5732) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15532) [M::mem_pestat] mean and std.dev: (3452.65, 2939.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20432) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (738, 2451, 5517) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15075) [M::mem_pestat] mean and std.dev: (3336.08, 2923.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19854) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 377.100 CPU sec, 37.163 real sec [M::process] read 5555556 sequences (200000016 bp)... [M::process] read 5555556 sequences (200000016 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24086, 946685, 23180, 23928) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (714, 2463, 5583) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15321) [M::mem_pestat] mean and std.dev: (3341.06, 2949.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20190) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 137, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 319) [M::mem_pestat] mean and std.dev: (144.83, 50.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 389) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (806, 2680, 5703) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15497) [M::mem_pestat] mean and std.dev: (3462.24, 2941.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20394) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (714, 2434, 5513) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15111) [M::mem_pestat] mean and std.dev: (3306.69, 2920.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19910) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 374.729 CPU sec, 39.752 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24073, 937937, 22746, 23665) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (708, 2448, 5571) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15297) [M::mem_pestat] mean and std.dev: (3328.97, 2932.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20160) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 138, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 322) [M::mem_pestat] mean and std.dev: (145.50, 51.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 393) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (808, 2667, 5714) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15526) [M::mem_pestat] mean and std.dev: (3457.87, 2942.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20432) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (724, 2475, 5553) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15211) [M::mem_pestat] mean and std.dev: (3346.67, 2940.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20040) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 378.474 CPU sec, 36.375 real sec [M::process] read 5555556 sequences (200000016 bp)... [M::process] read 5555556 sequences (200000016 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24061, 943054, 22499, 23810) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (721, 2506, 5632) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15454) [M::mem_pestat] mean and std.dev: (3373.73, 2956.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20365) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 138, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 319) [M::mem_pestat] mean and std.dev: (144.96, 50.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 389) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (837, 2674, 5681) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15369) [M::mem_pestat] mean and std.dev: (3452.69, 2921.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20213) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (763, 2535, 5600) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15274) [M::mem_pestat] mean and std.dev: (3378.57, 2930.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20111) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 372.246 CPU sec, 40.093 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23864, 946616, 23026, 23443) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (725, 2467, 5617) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15401) [M::mem_pestat] mean and std.dev: (3356.52, 2945.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20293) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 137, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 316) [M::mem_pestat] mean and std.dev: (144.52, 50.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 385) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (821, 2651, 5677) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15389) [M::mem_pestat] mean and std.dev: (3450.28, 2924.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20245) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (727, 2500, 5652) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15502) [M::mem_pestat] mean and std.dev: (3377.63, 2944.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20427) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 377.027 CPU sec, 35.538 real sec [M::process] read 5555556 sequences (200000016 bp)... [M::process] read 5555556 sequences (200000016 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23900, 941500, 22492, 23979) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (720, 2453, 5519) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15117) [M::mem_pestat] mean and std.dev: (3328.81, 2936.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19916) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 138, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 319) [M::mem_pestat] mean and std.dev: (145.21, 50.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 389) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (838, 2677, 5765) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15619) [M::mem_pestat] mean and std.dev: (3475.84, 2934.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20546) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (740, 2465, 5576) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15248) [M::mem_pestat] mean and std.dev: (3346.58, 2927.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20084) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 374.479 CPU sec, 38.972 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24166, 940569, 22805, 23920) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (719, 2454, 5597) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15353) [M::mem_pestat] mean and std.dev: (3349.30, 2945.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20231) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 137, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 319) [M::mem_pestat] mean and std.dev: (144.93, 50.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 389) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (834, 2670, 5753) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15591) [M::mem_pestat] mean and std.dev: (3469.17, 2938.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20510) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (731, 2485, 5693) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15617) [M::mem_pestat] mean and std.dev: (3387.58, 2961.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20579) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 371.864 CPU sec, 33.610 real sec [M::process] read 5555556 sequences (200000016 bp)... [M::process] read 5555556 sequences (200000016 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23822, 941928, 22794, 23758) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (702, 2409, 5584) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15348) [M::mem_pestat] mean and std.dev: (3329.36, 2941.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20230) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 137, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 319) [M::mem_pestat] mean and std.dev: (144.86, 50.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 389) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (807, 2617, 5735) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15591) [M::mem_pestat] mean and std.dev: (3447.71, 2953.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20519) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (748, 2551, 5628) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15388) [M::mem_pestat] mean and std.dev: (3394.51, 2950.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20268) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 352.045 CPU sec, 23.714 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23742, 942100, 22740, 23254) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (682, 2402, 5489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15103) [M::mem_pestat] mean and std.dev: (3298.21, 2935.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19910) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 137, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 319) [M::mem_pestat] mean and std.dev: (144.90, 50.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 389) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (797, 2639, 5766) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15704) [M::mem_pestat] mean and std.dev: (3456.52, 2943.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20673) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (710, 2431, 5582) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15326) [M::mem_pestat] mean and std.dev: (3323.21, 2929.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20198) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 357.810 CPU sec, 26.855 real sec [M::process] read 5555556 sequences (200000016 bp)... [M::process] read 5555556 sequences (200000016 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23761, 940532, 22877, 23683) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (726, 2476, 5608) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15372) [M::mem_pestat] mean and std.dev: (3356.99, 2950.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20254) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 138, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 319) [M::mem_pestat] mean and std.dev: (145.24, 50.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 389) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (832, 2669, 5743) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15565) [M::mem_pestat] mean and std.dev: (3466.85, 2941.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20476) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (753, 2556, 5610) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15324) [M::mem_pestat] mean and std.dev: (3382.28, 2936.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20181) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 381.792 CPU sec, 40.630 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20933, 1116558, 17847, 18893) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (385, 1965, 5113) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14569) [M::mem_pestat] mean and std.dev: (3013.70, 2950.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19297) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 163, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 456) [M::mem_pestat] mean and std.dev: (180.24, 80.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 568) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (813, 2587, 5667) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15375) [M::mem_pestat] mean and std.dev: (3427.79, 2932.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20229) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (708, 2462, 5504) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15096) [M::mem_pestat] mean and std.dev: (3327.99, 2943.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19892) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 382.379 CPU sec, 36.692 real sec [M::process] read 5555556 sequences (200000016 bp)... [M::process] read 5555556 sequences (200000016 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10727, 1714007, 1391, 1848) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 145, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 495) [M::mem_pestat] mean and std.dev: (153.70, 94.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 628) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (180, 238, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 549) [M::mem_pestat] mean and std.dev: (245.98, 88.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 672) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (456, 1982, 5545) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15723) [M::mem_pestat] mean and std.dev: (3127.87, 3012.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20812) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (123, 368, 2534) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7356) [M::mem_pestat] mean and std.dev: (1266.40, 1838.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9767) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 393.284 CPU sec, 44.537 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10695, 1715509, 1447, 1794) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 141, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 482) [M::mem_pestat] mean and std.dev: (150.03, 91.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 611) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (180, 238, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 549) [M::mem_pestat] mean and std.dev: (245.71, 88.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 672) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (453, 1911, 5363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15183) [M::mem_pestat] mean and std.dev: (3064.80, 2976.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20093) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (126, 386, 2436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7056) [M::mem_pestat] mean and std.dev: (1224.52, 1738.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9366) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 382.922 CPU sec, 34.559 real sec [M::process] read 5555556 sequences (200000016 bp)... [M::process] read 5555556 sequences (200000016 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10642, 1715677, 1367, 1896) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 142, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (151.59, 92.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 630) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (180, 238, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 549) [M::mem_pestat] mean and std.dev: (246.28, 89.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 672) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (496, 2056, 5592) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15784) [M::mem_pestat] mean and std.dev: (3110.91, 2944.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20880) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (125, 369, 2437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7061) [M::mem_pestat] mean and std.dev: (1214.35, 1763.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9373) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 389.274 CPU sec, 43.912 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10741, 1715263, 1374, 1856) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 143, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 489) [M::mem_pestat] mean and std.dev: (151.77, 92.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 620) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (180, 238, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 549) [M::mem_pestat] mean and std.dev: (245.83, 88.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 672) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (368, 1965, 5554) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15926) [M::mem_pestat] mean and std.dev: (3111.36, 3063.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21112) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (131, 366, 2264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6530) [M::mem_pestat] mean and std.dev: (1112.49, 1636.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8663) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 385.924 CPU sec, 36.413 real sec [M::process] read 5555556 sequences (200000016 bp)... [M::process] read 5555556 sequences (200000016 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10709, 1716331, 1416, 1746) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 142, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (150.37, 92.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 610) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (180, 237, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 549) [M::mem_pestat] mean and std.dev: (245.61, 88.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 672) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (385, 1768, 5098) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14524) [M::mem_pestat] mean and std.dev: (2944.20, 2991.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19237) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (118, 325, 1992) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5740) [M::mem_pestat] mean and std.dev: (927.48, 1386.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7614) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 389.970 CPU sec, 42.633 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10777, 1717410, 1432, 1788) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 141, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 479) [M::mem_pestat] mean and std.dev: (150.89, 90.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 607) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (179, 237, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 548) [M::mem_pestat] mean and std.dev: (245.12, 88.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 671) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (412, 1859, 5422) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15442) [M::mem_pestat] mean and std.dev: (3046.77, 2981.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20452) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (133, 413, 2466) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7132) [M::mem_pestat] mean and std.dev: (1239.83, 1784.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9465) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 381.589 CPU sec, 35.371 real sec [M::process] read 5555556 sequences (200000016 bp)... [M::process] read 5555556 sequences (200000016 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11012, 1714327, 1445, 1771) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 139, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 468) [M::mem_pestat] mean and std.dev: (147.71, 90.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (179, 237, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 548) [M::mem_pestat] mean and std.dev: (244.92, 88.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 671) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (452, 2067, 5319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15053) [M::mem_pestat] mean and std.dev: (3096.97, 2956.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19920) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (120, 368, 2480) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7200) [M::mem_pestat] mean and std.dev: (1261.12, 1821.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9560) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 391.109 CPU sec, 45.191 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10745, 1710676, 1337, 1839) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 142, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (150.62, 91.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (179, 237, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 551) [M::mem_pestat] mean and std.dev: (245.59, 89.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 675) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (421, 1854, 5095) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14443) [M::mem_pestat] mean and std.dev: (2951.51, 2928.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19117) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (130, 362, 2207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6361) [M::mem_pestat] mean and std.dev: (1065.31, 1568.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8438) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 383.111 CPU sec, 36.176 real sec [M::process] read 5555556 sequences (200000016 bp)... [M::process] read 5298184 sequences (200000048 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10933, 1711597, 1352, 1707) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 141, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 479) [M::mem_pestat] mean and std.dev: (150.05, 90.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 607) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (179, 237, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 548) [M::mem_pestat] mean and std.dev: (244.94, 88.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 671) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (436, 1913, 5502) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15634) [M::mem_pestat] mean and std.dev: (3116.04, 3043.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20700) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (124, 342, 2258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6526) [M::mem_pestat] mean and std.dev: (1110.47, 1620.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8660) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 395.246 CPU sec, 45.628 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8120, 1549852, 6249, 6800) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (414, 2044, 5070) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14382) [M::mem_pestat] mean and std.dev: (3014.26, 2900.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19038) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 198, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 548) [M::mem_pestat] mean and std.dev: (214.23, 97.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 684) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1593, 3557, 6358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15888) [M::mem_pestat] mean and std.dev: (4060.26, 2855.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20653) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1019, 2895, 5865) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15557) [M::mem_pestat] mean and std.dev: (3602.67, 2904.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20403) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5298184 reads in 296.965 CPU sec, 19.841 real sec [M::process] read 5263158 sequences (200000004 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7797, 1524439, 6932, 7563) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (981, 2868, 5815) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15483) [M::mem_pestat] mean and std.dev: (3570.80, 2893.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20317) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 191, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 540) [M::mem_pestat] mean and std.dev: (209.18, 97.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 675) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1551, 3524, 6290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15768) [M::mem_pestat] mean and std.dev: (4019.21, 2824.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20507) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1031, 2899, 5854) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15500) [M::mem_pestat] mean and std.dev: (3606.41, 2897.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20323) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5263158 reads in 286.682 CPU sec, 19.874 real sec [M::process] read 5263158 sequences (200000004 bp)... [M::process] read 5263158 sequences (200000004 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7645, 1524694, 6873, 7549) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1029, 2889, 5937) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15753) [M::mem_pestat] mean and std.dev: (3617.22, 2910.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20661) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 190, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 533) [M::mem_pestat] mean and std.dev: (207.34, 96.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 666) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1551, 3418, 6120) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15258) [M::mem_pestat] mean and std.dev: (3968.66, 2823.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19827) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1069, 2772, 5560) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14542) [M::mem_pestat] mean and std.dev: (3503.74, 2825.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19033) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5263158 reads in 292.264 CPU sec, 19.494 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7706, 1524951, 6833, 7544) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1005, 2855, 5722) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15156) [M::mem_pestat] mean and std.dev: (3560.36, 2876.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19873) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 190, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 536) [M::mem_pestat] mean and std.dev: (208.04, 96.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 670) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1533, 3480, 6277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15765) [M::mem_pestat] mean and std.dev: (4022.50, 2849.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20509) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1027, 2845, 5763) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15235) [M::mem_pestat] mean and std.dev: (3575.96, 2873.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19971) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5263158 reads in 287.854 CPU sec, 18.955 real sec [M::process] read 5263158 sequences (200000004 bp)... [M::process] read 5263158 sequences (200000004 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7521, 1522613, 6723, 7490) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (982, 2750, 5680) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15076) [M::mem_pestat] mean and std.dev: (3522.22, 2886.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19774) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 191, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 537) [M::mem_pestat] mean and std.dev: (208.79, 97.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 671) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1549, 3557, 6248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15646) [M::mem_pestat] mean and std.dev: (4029.64, 2834.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20345) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1015, 2846, 5791) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15343) [M::mem_pestat] mean and std.dev: (3588.46, 2911.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20119) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5263158 reads in 288.122 CPU sec, 25.692 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7610, 1521714, 6862, 7715) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (939, 2851, 5785) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15477) [M::mem_pestat] mean and std.dev: (3562.11, 2894.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20323) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 192, 271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 543) [M::mem_pestat] mean and std.dev: (210.05, 98.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 679) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1570, 3495, 6180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15400) [M::mem_pestat] mean and std.dev: (3994.39, 2833.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20010) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (981, 2928, 5798) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15432) [M::mem_pestat] mean and std.dev: (3594.90, 2900.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20249) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5263158 reads in 318.013 CPU sec, 37.133 real sec [M::process] read 5263158 sequences (200000004 bp)... [M::process] read 5263158 sequences (200000004 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7637, 1521985, 6963, 7498) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (983, 2748, 5850) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15584) [M::mem_pestat] mean and std.dev: (3559.86, 2930.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20451) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 191, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 540) [M::mem_pestat] mean and std.dev: (209.11, 97.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 675) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1469, 3472, 6229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15749) [M::mem_pestat] mean and std.dev: (3967.78, 2845.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20509) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1087, 2892, 5738) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15040) [M::mem_pestat] mean and std.dev: (3600.43, 2861.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19691) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5263158 reads in 288.093 CPU sec, 24.598 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7854, 1523524, 6783, 7431) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (970, 2745, 5686) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15118) [M::mem_pestat] mean and std.dev: (3511.71, 2876.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19834) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 191, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 537) [M::mem_pestat] mean and std.dev: (208.62, 97.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 671) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1589, 3535, 6309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15749) [M::mem_pestat] mean and std.dev: (4032.28, 2839.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20469) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1025, 2985, 5840) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15470) [M::mem_pestat] mean and std.dev: (3618.35, 2885.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20285) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5263158 reads in 319.021 CPU sec, 36.869 real sec [M::process] read 5263158 sequences (200000004 bp)... [M::process] read 5263158 sequences (200000004 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7636, 1523383, 6742, 7461) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (980, 2811, 5816) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15488) [M::mem_pestat] mean and std.dev: (3560.31, 2889.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20324) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 191, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 539) [M::mem_pestat] mean and std.dev: (208.59, 97.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 674) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1545, 3527, 6284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15762) [M::mem_pestat] mean and std.dev: (4012.69, 2830.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20501) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1002, 2816, 5836) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15504) [M::mem_pestat] mean and std.dev: (3585.10, 2892.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20338) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5263158 reads in 288.017 CPU sec, 25.488 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7514, 1526119, 6938, 7595) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1010, 2777, 5728) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15164) [M::mem_pestat] mean and std.dev: (3554.40, 2890.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19882) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 190, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 536) [M::mem_pestat] mean and std.dev: (208.02, 97.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 670) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1575, 3553, 6280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15690) [M::mem_pestat] mean and std.dev: (4042.17, 2835.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20395) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1063, 2841, 5881) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15517) [M::mem_pestat] mean and std.dev: (3620.19, 2906.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20335) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5263158 reads in 322.005 CPU sec, 37.523 real sec [M::process] read 5263158 sequences (200000004 bp)... [M::process] read 5263158 sequences (200000004 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7604, 1525544, 6904, 7551) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (930, 2688, 5664) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15132) [M::mem_pestat] mean and std.dev: (3476.71, 2866.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19866) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 190, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 536) [M::mem_pestat] mean and std.dev: (207.76, 96.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 670) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1608, 3480, 6278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15618) [M::mem_pestat] mean and std.dev: (4027.35, 2835.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20288) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1058, 2907, 5885) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15539) [M::mem_pestat] mean and std.dev: (3634.09, 2904.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20366) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5263158 reads in 296.103 CPU sec, 26.444 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7623, 1527534, 6755, 7627) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (976, 2903, 5873) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15667) [M::mem_pestat] mean and std.dev: (3610.99, 2925.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20564) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 190, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 533) [M::mem_pestat] mean and std.dev: (207.31, 96.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 666) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1527, 3527, 6333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15945) [M::mem_pestat] mean and std.dev: (4035.10, 2866.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20751) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1058, 2957, 5841) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15407) [M::mem_pestat] mean and std.dev: (3632.06, 2886.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20190) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5263158 reads in 326.084 CPU sec, 36.960 real sec [M::process] read 5263158 sequences (200000004 bp)... [M::process] read 5263158 sequences (200000004 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7606, 1524789, 6770, 7722) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (994, 2844, 5805) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15427) [M::mem_pestat] mean and std.dev: (3569.75, 2883.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20238) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 190, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 536) [M::mem_pestat] mean and std.dev: (207.71, 96.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 670) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1604, 3538, 6214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15434) [M::mem_pestat] mean and std.dev: (4016.67, 2802.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20044) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1009, 2786, 5711) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15115) [M::mem_pestat] mean and std.dev: (3532.14, 2864.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19817) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5263158 reads in 292.540 CPU sec, 24.619 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7693, 1522294, 6870, 7447) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (953, 2727, 5826) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15572) [M::mem_pestat] mean and std.dev: (3517.02, 2889.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20445) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 190, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 539) [M::mem_pestat] mean and std.dev: (208.27, 97.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 674) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1606, 3564, 6317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15739) [M::mem_pestat] mean and std.dev: (4054.76, 2838.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20450) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1053, 2925, 5829) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15381) [M::mem_pestat] mean and std.dev: (3623.67, 2889.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20157) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5263158 reads in 319.460 CPU sec, 33.784 real sec [M::process] read 5263158 sequences (200000004 bp)... [M::process] read 5263158 sequences (200000004 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7745, 1526460, 6866, 7623) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1013, 2795, 5683) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15023) [M::mem_pestat] mean and std.dev: (3538.56, 2876.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19693) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 190, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 536) [M::mem_pestat] mean and std.dev: (207.83, 96.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 670) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1519, 3460, 6220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15622) [M::mem_pestat] mean and std.dev: (3986.02, 2834.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20323) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1090, 2961, 5840) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15340) [M::mem_pestat] mean and std.dev: (3645.29, 2883.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20090) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5263158 reads in 295.165 CPU sec, 25.313 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7570, 1526017, 6867, 7392) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (970, 2837, 5783) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15409) [M::mem_pestat] mean and std.dev: (3569.34, 2899.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20222) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 190, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 536) [M::mem_pestat] mean and std.dev: (207.61, 96.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 670) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1586, 3429, 6155) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15293) [M::mem_pestat] mean and std.dev: (3980.54, 2820.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19862) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (995, 2847, 5904) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15722) [M::mem_pestat] mean and std.dev: (3595.90, 2907.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20631) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5263158 reads in 326.564 CPU sec, 36.945 real sec [M::process] read 5263158 sequences (200000004 bp)... [M::process] read 1614076 sequences (200000072 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7733, 1526288, 6903, 7698) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1029, 2859, 5706) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15060) [M::mem_pestat] mean and std.dev: (3565.55, 2865.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19737) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 190, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 536) [M::mem_pestat] mean and std.dev: (207.68, 96.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 670) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1564, 3507, 6244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15604) [M::mem_pestat] mean and std.dev: (4007.15, 2846.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20284) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1022, 2878, 5801) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15359) [M::mem_pestat] mean and std.dev: (3592.91, 2894.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20138) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5263158 reads in 293.496 CPU sec, 24.667 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1373, 534764, 1117, 1356) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (703, 2343, 5414) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14836) [M::mem_pestat] mean and std.dev: (3198.36, 2823.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19547) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 191, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 580) [M::mem_pestat] mean and std.dev: (212.38, 109.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 730) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1446, 3338, 6120) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15468) [M::mem_pestat] mean and std.dev: (3920.09, 2857.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20142) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (845, 2620, 5664) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15302) [M::mem_pestat] mean and std.dev: (3473.98, 2957.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20121) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1614076 reads in 218.589 CPU sec, 21.411 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (913, 458062, 757, 875) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (647, 2233, 5053) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13865) [M::mem_pestat] mean and std.dev: (3159.51, 2907.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18271) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 190, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 587) [M::mem_pestat] mean and std.dev: (212.49, 111.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 740) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1264, 3046, 6036) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15580) [M::mem_pestat] mean and std.dev: (3753.05, 2850.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20352) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (660, 2541, 5580) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15420) [M::mem_pestat] mean and std.dev: (3359.85, 2916.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20340) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 193.546 CPU sec, 12.564 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (918, 457942, 778, 875) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (691, 2631, 5652) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15574) [M::mem_pestat] mean and std.dev: (3371.60, 2913.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20535) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 189, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 584) [M::mem_pestat] mean and std.dev: (211.91, 111.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 736) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1052, 3019, 5836) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15404) [M::mem_pestat] mean and std.dev: (3658.13, 2920.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20188) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (843, 2417, 5637) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15225) [M::mem_pestat] mean and std.dev: (3310.30, 2826.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20019) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 193.164 CPU sec, 12.338 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (915, 457799, 840, 877) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (835, 2566, 5585) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15085) [M::mem_pestat] mean and std.dev: (3365.84, 2866.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19835) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 190, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 587) [M::mem_pestat] mean and std.dev: (212.50, 111.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 740) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (958, 3176, 5715) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15229) [M::mem_pestat] mean and std.dev: (3608.93, 2858.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19986) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (659, 2176, 5468) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15086) [M::mem_pestat] mean and std.dev: (3209.85, 2945.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19895) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 196.952 CPU sec, 12.819 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (928, 457236, 760, 892) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (668, 2334, 5718) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15818) [M::mem_pestat] mean and std.dev: (3312.68, 2976.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20868) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 190, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 587) [M::mem_pestat] mean and std.dev: (212.67, 111.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 740) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1072, 3349, 6493) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 17335) [M::mem_pestat] mean and std.dev: (3884.59, 2993.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 22756) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (671, 2381, 5530) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15248) [M::mem_pestat] mean and std.dev: (3295.20, 2893.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20107) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 195.912 CPU sec, 12.759 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (907, 457777, 743, 947) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (725, 2631, 5858) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16124) [M::mem_pestat] mean and std.dev: (3471.36, 3020.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21257) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 190, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588) [M::mem_pestat] mean and std.dev: (213.08, 111.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 741) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1377, 3264, 6128) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15630) [M::mem_pestat] mean and std.dev: (3846.31, 2856.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20381) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (695, 2500, 5497) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15101) [M::mem_pestat] mean and std.dev: (3270.71, 2838.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19903) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 195.443 CPU sec, 12.692 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (864, 457938, 818, 837) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (765, 2303, 5165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13965) [M::mem_pestat] mean and std.dev: (3176.74, 2770.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18365) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 190, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588) [M::mem_pestat] mean and std.dev: (213.18, 112.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 741) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (814, 2814, 5749) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15619) [M::mem_pestat] mean and std.dev: (3575.69, 2917.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20554) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (689, 2403, 5647) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15563) [M::mem_pestat] mean and std.dev: (3348.39, 2966.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20521) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 196.361 CPU sec, 12.940 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (974, 458251, 759, 870) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (644, 2381, 5278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14546) [M::mem_pestat] mean and std.dev: (3216.75, 2867.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19180) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 190, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588) [M::mem_pestat] mean and std.dev: (213.39, 112.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 741) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1217, 3188, 6480) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 17006) [M::mem_pestat] mean and std.dev: (3868.82, 3026.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 22269) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (699, 2645, 5472) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15018) [M::mem_pestat] mean and std.dev: (3343.66, 2860.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19791) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 195.757 CPU sec, 12.660 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (903, 458746, 744, 963) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (706, 2524, 5289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14455) [M::mem_pestat] mean and std.dev: (3311.15, 2874.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19038) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 191, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591) [M::mem_pestat] mean and std.dev: (213.79, 112.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 745) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1180, 3369, 6193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16219) [M::mem_pestat] mean and std.dev: (3853.63, 2883.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21232) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (679, 2116, 5536) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15250) [M::mem_pestat] mean and std.dev: (3215.02, 2901.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20107) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 193.825 CPU sec, 12.489 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (945, 458367, 786, 807) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (719, 2555, 5419) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14819) [M::mem_pestat] mean and std.dev: (3336.75, 2885.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19519) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 191, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594) [M::mem_pestat] mean and std.dev: (214.78, 113.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 749) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1042, 2961, 5624) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14788) [M::mem_pestat] mean and std.dev: (3570.66, 2816.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19370) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (720, 2545, 5432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14856) [M::mem_pestat] mean and std.dev: (3336.94, 2889.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19568) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 193.182 CPU sec, 12.541 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (951, 458058, 746, 927) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (769, 2406, 5612) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15298) [M::mem_pestat] mean and std.dev: (3330.41, 2938.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20141) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 192, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595) [M::mem_pestat] mean and std.dev: (215.36, 113.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 750) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1322, 3413, 6169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15863) [M::mem_pestat] mean and std.dev: (3824.42, 2833.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20710) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (776, 2611, 5183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13997) [M::mem_pestat] mean and std.dev: (3295.60, 2862.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18404) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 194.353 CPU sec, 12.788 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (949, 457897, 797, 919) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (816, 2575, 5441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14691) [M::mem_pestat] mean and std.dev: (3311.96, 2835.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19316) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 192, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594) [M::mem_pestat] mean and std.dev: (214.94, 113.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 749) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1072, 2949, 5758) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15130) [M::mem_pestat] mean and std.dev: (3600.35, 2856.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19816) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (715, 2618, 5294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14452) [M::mem_pestat] mean and std.dev: (3304.50, 2831.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19031) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 195.678 CPU sec, 12.710 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (925, 457830, 851, 929) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (742, 2471, 5533) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15115) [M::mem_pestat] mean and std.dev: (3331.43, 2866.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19906) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 191, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591) [M::mem_pestat] mean and std.dev: (214.32, 112.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 745) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1154, 2964, 5698) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14786) [M::mem_pestat] mean and std.dev: (3643.67, 2795.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19330) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (702, 2251, 5562) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15282) [M::mem_pestat] mean and std.dev: (3291.29, 2916.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20142) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 193.421 CPU sec, 12.893 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (985, 458937, 767, 876) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (814, 2442, 5657) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15343) [M::mem_pestat] mean and std.dev: (3378.22, 2930.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20186) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 190, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588) [M::mem_pestat] mean and std.dev: (213.20, 111.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 741) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1170, 3027, 5966) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15558) [M::mem_pestat] mean and std.dev: (3716.46, 2927.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20354) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (724, 2253, 5511) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15085) [M::mem_pestat] mean and std.dev: (3278.15, 2876.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19872) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 194.317 CPU sec, 12.617 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (798, 458522, 798, 851) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (696, 2697, 5853) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16167) [M::mem_pestat] mean and std.dev: (3502.52, 3016.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21324) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 190, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588) [M::mem_pestat] mean and std.dev: (213.18, 111.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 741) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1299, 3224, 5595) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14187) [M::mem_pestat] mean and std.dev: (3706.99, 2758.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18483) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (845, 2870, 5621) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15173) [M::mem_pestat] mean and std.dev: (3459.84, 2845.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19949) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 194.186 CPU sec, 12.349 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (924, 457692, 789, 896) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (763, 2519, 5324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14446) [M::mem_pestat] mean and std.dev: (3296.82, 2851.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19007) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 190, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588) [M::mem_pestat] mean and std.dev: (213.25, 111.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 741) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1376, 3422, 6525) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16823) [M::mem_pestat] mean and std.dev: (4014.39, 2983.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21972) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (797, 2723, 5608) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15230) [M::mem_pestat] mean and std.dev: (3453.43, 2908.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20041) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 193.953 CPU sec, 12.578 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (946, 457875, 762, 894) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (695, 2518, 5505) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15125) [M::mem_pestat] mean and std.dev: (3329.45, 2903.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19935) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 191, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591) [M::mem_pestat] mean and std.dev: (214.00, 112.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 745) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1318, 3151, 5782) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14710) [M::mem_pestat] mean and std.dev: (3715.91, 2777.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19174) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (725, 2404, 5614) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15392) [M::mem_pestat] mean and std.dev: (3324.91, 2909.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20281) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 193.529 CPU sec, 12.710 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (943, 457726, 803, 884) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (689, 2706, 5800) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16022) [M::mem_pestat] mean and std.dev: (3445.50, 3024.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21133) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 190, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588) [M::mem_pestat] mean and std.dev: (213.52, 112.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 741) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1265, 3050, 6034) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15572) [M::mem_pestat] mean and std.dev: (3721.33, 2828.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20341) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (746, 2488, 5549) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15155) [M::mem_pestat] mean and std.dev: (3354.24, 2901.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19958) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 196.080 CPU sec, 12.719 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (922, 458036, 810, 935) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (670, 2228, 5367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14761) [M::mem_pestat] mean and std.dev: (3229.21, 2924.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19458) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 191, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588) [M::mem_pestat] mean and std.dev: (213.31, 111.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 741) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1371, 3410, 5956) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15126) [M::mem_pestat] mean and std.dev: (3818.45, 2820.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19711) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (815, 2644, 5530) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14960) [M::mem_pestat] mean and std.dev: (3440.57, 2918.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19675) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 192.945 CPU sec, 12.255 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (885, 457476, 747, 862) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (612, 2133, 5422) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15042) [M::mem_pestat] mean and std.dev: (3217.27, 2933.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19852) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 191, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591) [M::mem_pestat] mean and std.dev: (213.92, 112.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 745) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1116, 3057, 5936) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15576) [M::mem_pestat] mean and std.dev: (3629.24, 2887.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20396) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (738, 2445, 5835) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16029) [M::mem_pestat] mean and std.dev: (3388.03, 2951.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21126) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 195.499 CPU sec, 12.804 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (870, 459072, 744, 867) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (676, 2492, 5867) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16249) [M::mem_pestat] mean and std.dev: (3376.37, 2970.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21440) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 190, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 590) [M::mem_pestat] mean and std.dev: (213.31, 112.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 744) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1307, 3222, 6195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15971) [M::mem_pestat] mean and std.dev: (3852.81, 2866.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20859) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (731, 2349, 5357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14609) [M::mem_pestat] mean and std.dev: (3258.28, 2896.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19235) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 193.388 CPU sec, 12.718 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (893, 458671, 730, 952) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (624, 2247, 5280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14592) [M::mem_pestat] mean and std.dev: (3172.93, 2874.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19248) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 190, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 587) [M::mem_pestat] mean and std.dev: (212.72, 111.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 740) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1222, 3506, 6182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16102) [M::mem_pestat] mean and std.dev: (3894.54, 2933.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21062) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (865, 2498, 5724) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15442) [M::mem_pestat] mean and std.dev: (3451.89, 2903.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20301) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 193.335 CPU sec, 12.505 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (849, 458626, 800, 804) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (648, 2427, 5282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14550) [M::mem_pestat] mean and std.dev: (3229.44, 2856.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19184) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 189, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 584) [M::mem_pestat] mean and std.dev: (211.75, 110.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 736) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1123, 3039, 5916) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15502) [M::mem_pestat] mean and std.dev: (3634.30, 2876.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20295) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (777, 2534, 5724) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15618) [M::mem_pestat] mean and std.dev: (3394.29, 2923.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20565) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 193.393 CPU sec, 12.307 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (865, 458033, 758, 932) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (646, 2546, 5768) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16012) [M::mem_pestat] mean and std.dev: (3390.71, 3008.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21134) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 188, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 581) [M::mem_pestat] mean and std.dev: (210.74, 110.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 732) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1165, 3240, 6033) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15769) [M::mem_pestat] mean and std.dev: (3780.85, 2920.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20637) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (617, 2327, 5133) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14165) [M::mem_pestat] mean and std.dev: (3201.62, 2862.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18681) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 196.796 CPU sec, 12.769 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (885, 457993, 744, 810) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (719, 2442, 5302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14468) [M::mem_pestat] mean and std.dev: (3298.79, 2873.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19051) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (127, 187, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 580) [M::mem_pestat] mean and std.dev: (210.34, 110.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 731) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1161, 3290, 6030) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15768) [M::mem_pestat] mean and std.dev: (3788.89, 2902.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20637) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (655, 2348, 5186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14248) [M::mem_pestat] mean and std.dev: (3228.83, 2890.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18779) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 194.602 CPU sec, 12.412 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (884, 458879, 752, 882) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (644, 2347, 5475) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15137) [M::mem_pestat] mean and std.dev: (3297.39, 2992.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19968) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (127, 188, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 580) [M::mem_pestat] mean and std.dev: (210.23, 110.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 731) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1149, 3027, 5944) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15534) [M::mem_pestat] mean and std.dev: (3739.09, 2983.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20329) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (817, 2761, 5949) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16213) [M::mem_pestat] mean and std.dev: (3602.35, 3020.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21345) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 194.856 CPU sec, 12.665 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (918, 458137, 716, 907) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (712, 2648, 5729) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15763) [M::mem_pestat] mean and std.dev: (3447.91, 3001.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20780) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (127, 188, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 580) [M::mem_pestat] mean and std.dev: (210.05, 109.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 731) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1157, 2980, 5973) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15605) [M::mem_pestat] mean and std.dev: (3752.98, 2952.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20421) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (830, 2679, 5493) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14819) [M::mem_pestat] mean and std.dev: (3383.70, 2870.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19482) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 194.684 CPU sec, 12.552 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (906, 458540, 789, 851) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (651, 2582, 5908) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16422) [M::mem_pestat] mean and std.dev: (3437.77, 2992.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21679) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 188, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 578) [M::mem_pestat] mean and std.dev: (210.44, 109.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 728) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1134, 3430, 6270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16542) [M::mem_pestat] mean and std.dev: (3872.99, 3015.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21678) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (669, 2445, 5560) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15342) [M::mem_pestat] mean and std.dev: (3324.27, 2887.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20233) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 197.950 CPU sec, 13.099 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (946, 458322, 835, 843) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (748, 2564, 5478) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14938) [M::mem_pestat] mean and std.dev: (3392.83, 2961.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19668) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 191, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591) [M::mem_pestat] mean and std.dev: (213.95, 112.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 745) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1313, 3380, 6035) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15479) [M::mem_pestat] mean and std.dev: (3843.02, 2880.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20201) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (632, 2439, 5417) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14987) [M::mem_pestat] mean and std.dev: (3236.16, 2852.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19772) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 193.866 CPU sec, 12.342 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (874, 457202, 774, 864) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (694, 2583, 5757) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15883) [M::mem_pestat] mean and std.dev: (3359.91, 2912.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20946) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 190, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 590) [M::mem_pestat] mean and std.dev: (213.16, 112.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 744) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1089, 3151, 5664) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14814) [M::mem_pestat] mean and std.dev: (3673.65, 2818.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19389) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (696, 2765, 5477) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15039) [M::mem_pestat] mean and std.dev: (3422.12, 2936.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19820) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 195.432 CPU sec, 12.649 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (943, 456849, 827, 869) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (615, 2366, 5329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14757) [M::mem_pestat] mean and std.dev: (3177.31, 2840.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19471) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 190, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588) [M::mem_pestat] mean and std.dev: (213.44, 112.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 741) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1137, 3181, 5860) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15306) [M::mem_pestat] mean and std.dev: (3782.18, 2874.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20029) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (636, 2492, 5464) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15120) [M::mem_pestat] mean and std.dev: (3331.72, 2922.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19948) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 194.256 CPU sec, 12.448 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (912, 457918, 715, 935) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (671, 2466, 5648) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15602) [M::mem_pestat] mean and std.dev: (3293.93, 2926.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20579) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 190, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588) [M::mem_pestat] mean and std.dev: (213.40, 112.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 741) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1266, 3039, 6206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16086) [M::mem_pestat] mean and std.dev: (3763.88, 2854.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21026) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (785, 2485, 5413) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14669) [M::mem_pestat] mean and std.dev: (3322.09, 2882.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19297) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 193.905 CPU sec, 12.776 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (886, 458744, 763, 907) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (647, 2357, 5136) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14114) [M::mem_pestat] mean and std.dev: (3144.33, 2790.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18603) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 190, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588) [M::mem_pestat] mean and std.dev: (213.00, 111.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 741) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1327, 3265, 6138) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15760) [M::mem_pestat] mean and std.dev: (3847.28, 2899.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20571) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (759, 2444, 5478) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14916) [M::mem_pestat] mean and std.dev: (3353.56, 2952.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19635) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 193.454 CPU sec, 12.792 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (869, 458205, 778, 935) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (650, 2087, 5267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14501) [M::mem_pestat] mean and std.dev: (3105.35, 2870.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19118) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 191, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591) [M::mem_pestat] mean and std.dev: (213.95, 112.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 745) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1096, 3031, 5793) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15187) [M::mem_pestat] mean and std.dev: (3627.89, 2808.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19884) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (777, 2472, 5586) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15204) [M::mem_pestat] mean and std.dev: (3367.81, 2902.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20013) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 194.455 CPU sec, 12.611 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (908, 458285, 770, 915) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (806, 2413, 5477) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14819) [M::mem_pestat] mean and std.dev: (3263.27, 2869.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19490) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 191, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594) [M::mem_pestat] mean and std.dev: (214.49, 112.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 749) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1441, 3244, 5876) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14746) [M::mem_pestat] mean and std.dev: (3787.08, 2782.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19181) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (754, 2519, 5440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14812) [M::mem_pestat] mean and std.dev: (3308.65, 2820.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19498) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 192.265 CPU sec, 12.780 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (895, 457739, 788, 851) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (702, 2465, 5405) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14811) [M::mem_pestat] mean and std.dev: (3286.50, 2874.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19514) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 191, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594) [M::mem_pestat] mean and std.dev: (214.67, 113.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 749) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1405, 3361, 6336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16198) [M::mem_pestat] mean and std.dev: (3961.26, 2940.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21129) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (762, 2392, 5275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14301) [M::mem_pestat] mean and std.dev: (3186.62, 2834.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18814) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 194.350 CPU sec, 12.692 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (924, 458371, 784, 898) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (566, 2156, 5346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14906) [M::mem_pestat] mean and std.dev: (3125.54, 2921.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19686) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 191, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594) [M::mem_pestat] mean and std.dev: (214.68, 113.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 749) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1138, 2983, 5889) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15391) [M::mem_pestat] mean and std.dev: (3703.37, 2908.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20142) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (804, 2524, 5301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14295) [M::mem_pestat] mean and std.dev: (3335.08, 2854.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18792) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 192.437 CPU sec, 12.209 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (964, 457623, 867, 884) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (726, 2354, 5223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14217) [M::mem_pestat] mean and std.dev: (3238.08, 2894.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18714) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 192, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 592) [M::mem_pestat] mean and std.dev: (214.67, 112.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 746) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1268, 3124, 5678) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14498) [M::mem_pestat] mean and std.dev: (3658.62, 2736.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18908) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (822, 2626, 5786) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15714) [M::mem_pestat] mean and std.dev: (3474.92, 2918.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20678) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 193.326 CPU sec, 12.663 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (902, 458284, 782, 888) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (749, 2317, 5211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14135) [M::mem_pestat] mean and std.dev: (3263.37, 2933.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18597) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 192, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595) [M::mem_pestat] mean and std.dev: (215.23, 113.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 750) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1010, 2749, 5643) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14909) [M::mem_pestat] mean and std.dev: (3526.48, 2879.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19542) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (708, 2325, 5555) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15249) [M::mem_pestat] mean and std.dev: (3272.68, 2936.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20096) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 193.605 CPU sec, 12.712 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (886, 458989, 796, 878) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (795, 2430, 5771) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15723) [M::mem_pestat] mean and std.dev: (3421.05, 2981.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20699) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 192, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597) [M::mem_pestat] mean and std.dev: (215.07, 113.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1083, 3226, 5868) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15438) [M::mem_pestat] mean and std.dev: (3674.89, 2837.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20223) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (795, 2736, 5487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14871) [M::mem_pestat] mean and std.dev: (3391.12, 2866.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19563) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 191.629 CPU sec, 12.456 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (925, 457782, 798, 911) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (638, 2366, 5590) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15494) [M::mem_pestat] mean and std.dev: (3344.14, 3014.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20446) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 191, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594) [M::mem_pestat] mean and std.dev: (214.82, 113.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 749) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1244, 3006, 6142) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15938) [M::mem_pestat] mean and std.dev: (3763.42, 2921.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20836) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (650, 2441, 5362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14786) [M::mem_pestat] mean and std.dev: (3255.94, 2857.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19498) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 191.752 CPU sec, 12.211 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (883, 458352, 771, 947) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (702, 2396, 5454) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14958) [M::mem_pestat] mean and std.dev: (3290.20, 2958.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19710) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 191, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591) [M::mem_pestat] mean and std.dev: (213.87, 112.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 745) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1207, 3008, 6116) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15934) [M::mem_pestat] mean and std.dev: (3701.12, 2891.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20843) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (707, 2644, 5269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14393) [M::mem_pestat] mean and std.dev: (3285.95, 2836.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18955) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 192.738 CPU sec, 12.494 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (919, 458148, 781, 813) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (709, 2465, 5367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14683) [M::mem_pestat] mean and std.dev: (3283.73, 2873.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19341) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 190, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588) [M::mem_pestat] mean and std.dev: (213.02, 111.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 741) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1110, 2810, 5358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13854) [M::mem_pestat] mean and std.dev: (3469.82, 2802.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18102) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (786, 2556, 6043) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16557) [M::mem_pestat] mean and std.dev: (3428.26, 2933.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21814) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 192.739 CPU sec, 12.859 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (877, 458886, 771, 812) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (771, 2439, 5785) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15813) [M::mem_pestat] mean and std.dev: (3372.23, 2938.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20827) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 190, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 590) [M::mem_pestat] mean and std.dev: (213.25, 112.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 744) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1151, 3057, 5718) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14852) [M::mem_pestat] mean and std.dev: (3627.97, 2832.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19419) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (667, 2399, 5172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14182) [M::mem_pestat] mean and std.dev: (3220.93, 2876.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18687) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 193.533 CPU sec, 12.570 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (978, 458460, 736, 884) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (800, 2547, 5538) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15014) [M::mem_pestat] mean and std.dev: (3407.70, 2955.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19752) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 191, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591) [M::mem_pestat] mean and std.dev: (213.89, 112.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 745) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1092, 2787, 5612) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14652) [M::mem_pestat] mean and std.dev: (3531.26, 2830.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19172) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (808, 2397, 5607) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15205) [M::mem_pestat] mean and std.dev: (3350.83, 2913.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20004) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 192.261 CPU sec, 12.587 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (915, 458745, 826, 941) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (681, 2449, 5180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14178) [M::mem_pestat] mean and std.dev: (3244.66, 2892.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18677) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 190, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588) [M::mem_pestat] mean and std.dev: (213.30, 111.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 741) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1198, 3184, 5956) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15472) [M::mem_pestat] mean and std.dev: (3774.23, 2945.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20230) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (694, 2420, 5285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14467) [M::mem_pestat] mean and std.dev: (3242.12, 2876.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19058) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 192.282 CPU sec, 12.476 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (875, 458825, 720, 855) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (700, 2524, 5596) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15388) [M::mem_pestat] mean and std.dev: (3334.68, 2930.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20284) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 190, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588) [M::mem_pestat] mean and std.dev: (212.89, 111.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 741) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1335, 3201, 6194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15912) [M::mem_pestat] mean and std.dev: (3816.62, 2885.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20771) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (715, 2449, 5485) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15025) [M::mem_pestat] mean and std.dev: (3326.58, 2929.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19795) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 192.089 CPU sec, 12.226 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (904, 457726, 778, 943) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (622, 2407, 5358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14830) [M::mem_pestat] mean and std.dev: (3259.53, 2930.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19566) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 190, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 585) [M::mem_pestat] mean and std.dev: (212.76, 111.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 737) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1200, 3052, 5762) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14886) [M::mem_pestat] mean and std.dev: (3682.00, 2824.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19448) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (565, 2335, 5274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14692) [M::mem_pestat] mean and std.dev: (3209.42, 2937.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19401) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 194.247 CPU sec, 12.582 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (865, 458849, 753, 876) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (760, 2652, 5537) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15091) [M::mem_pestat] mean and std.dev: (3379.07, 2909.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19868) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 189, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 584) [M::mem_pestat] mean and std.dev: (211.91, 110.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 736) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1288, 3175, 6096) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15712) [M::mem_pestat] mean and std.dev: (3815.74, 2894.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20520) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (702, 2624, 5528) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15180) [M::mem_pestat] mean and std.dev: (3331.32, 2896.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20006) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 195.335 CPU sec, 12.931 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (935, 458371, 786, 902) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (605, 2456, 5161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14273) [M::mem_pestat] mean and std.dev: (3203.74, 2827.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18829) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 189, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 584) [M::mem_pestat] mean and std.dev: (211.53, 110.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 736) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1322, 3618, 6185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15911) [M::mem_pestat] mean and std.dev: (3973.10, 2923.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20774) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (644, 2164, 5229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14399) [M::mem_pestat] mean and std.dev: (3124.27, 2797.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18984) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 193.989 CPU sec, 12.575 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (864, 458117, 758, 895) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (709, 2384, 5500) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15082) [M::mem_pestat] mean and std.dev: (3263.59, 2851.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19873) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 189, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 584) [M::mem_pestat] mean and std.dev: (211.65, 110.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 736) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1172, 2908, 5965) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15551) [M::mem_pestat] mean and std.dev: (3729.78, 2917.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20344) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (668, 2096, 4941) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13487) [M::mem_pestat] mean and std.dev: (3066.33, 2790.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17760) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 195.369 CPU sec, 13.019 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (875, 459278, 736, 876) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (590, 2302, 5663) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15809) [M::mem_pestat] mean and std.dev: (3302.28, 3011.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20882) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (127, 188, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 580) [M::mem_pestat] mean and std.dev: (210.23, 110.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 731) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1166, 2963, 6004) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15680) [M::mem_pestat] mean and std.dev: (3705.97, 2887.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20518) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (618, 2310, 5463) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15153) [M::mem_pestat] mean and std.dev: (3295.68, 2952.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19998) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 191.793 CPU sec, 12.481 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (880, 458442, 735, 932) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (580, 2066, 5399) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15037) [M::mem_pestat] mean and std.dev: (3110.59, 2883.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19856) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (127, 188, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 580) [M::mem_pestat] mean and std.dev: (210.18, 110.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 731) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1263, 3059, 5419) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13731) [M::mem_pestat] mean and std.dev: (3579.25, 2755.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17887) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (782, 2503, 5165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13931) [M::mem_pestat] mean and std.dev: (3263.37, 2857.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18314) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 194.886 CPU sec, 12.763 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (894, 458050, 776, 945) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (638, 2249, 5095) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14009) [M::mem_pestat] mean and std.dev: (3196.49, 2875.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18466) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (127, 187, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 577) [M::mem_pestat] mean and std.dev: (209.71, 109.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 727) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (948, 2640, 5556) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14772) [M::mem_pestat] mean and std.dev: (3399.81, 2840.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19380) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (687, 2532, 5597) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15417) [M::mem_pestat] mean and std.dev: (3333.99, 2952.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20327) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 194.833 CPU sec, 12.633 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (894, 457789, 764, 876) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (747, 2415, 5759) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15783) [M::mem_pestat] mean and std.dev: (3374.39, 2961.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20795) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (127, 187, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 577) [M::mem_pestat] mean and std.dev: (209.74, 109.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 727) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1509, 3533, 6116) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15330) [M::mem_pestat] mean and std.dev: (3947.76, 2813.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19937) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (589, 2114, 5063) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14011) [M::mem_pestat] mean and std.dev: (3134.03, 2875.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18485) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 193.598 CPU sec, 12.315 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (908, 457458, 726, 897) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (665, 2149, 4996) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13658) [M::mem_pestat] mean and std.dev: (3057.97, 2807.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17989) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 189, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 581) [M::mem_pestat] mean and std.dev: (211.01, 110.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 732) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1041, 3217, 5905) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15633) [M::mem_pestat] mean and std.dev: (3699.76, 2890.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20497) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (710, 2253, 5370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14690) [M::mem_pestat] mean and std.dev: (3173.61, 2831.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19350) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 197.585 CPU sec, 12.778 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (916, 458452, 748, 887) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (804, 2725, 5594) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15174) [M::mem_pestat] mean and std.dev: (3411.94, 2940.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19964) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 192, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597) [M::mem_pestat] mean and std.dev: (215.26, 113.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1106, 3222, 6714) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 17930) [M::mem_pestat] mean and std.dev: (3880.55, 3059.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 23538) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (752, 2456, 5466) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14894) [M::mem_pestat] mean and std.dev: (3269.10, 2847.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19608) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 195.508 CPU sec, 12.465 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (851, 458344, 769, 869) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (633, 2259, 5270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14544) [M::mem_pestat] mean and std.dev: (3205.27, 2868.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19181) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 190, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591) [M::mem_pestat] mean and std.dev: (213.49, 112.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 745) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1201, 2931, 6183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16147) [M::mem_pestat] mean and std.dev: (3787.59, 2933.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21129) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (711, 2492, 5500) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15078) [M::mem_pestat] mean and std.dev: (3339.35, 2914.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19867) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 196.252 CPU sec, 12.723 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (928, 458178, 846, 905) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (585, 2384, 5238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14544) [M::mem_pestat] mean and std.dev: (3200.45, 2892.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19197) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 191, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591) [M::mem_pestat] mean and std.dev: (213.97, 112.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 745) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1306, 3277, 6475) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16813) [M::mem_pestat] mean and std.dev: (3872.53, 2969.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21982) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (707, 2623, 5759) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15863) [M::mem_pestat] mean and std.dev: (3409.76, 2929.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20915) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 196.296 CPU sec, 12.505 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (871, 458412, 818, 902) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (608, 2290, 5057) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13955) [M::mem_pestat] mean and std.dev: (3113.86, 2787.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18404) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 191, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591) [M::mem_pestat] mean and std.dev: (214.32, 112.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 745) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1094, 2889, 5772) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15128) [M::mem_pestat] mean and std.dev: (3659.74, 2924.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19806) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (687, 2336, 5434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14928) [M::mem_pestat] mean and std.dev: (3254.68, 2891.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19675) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 194.874 CPU sec, 12.448 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (968, 458654, 825, 851) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (702, 2532, 5311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14529) [M::mem_pestat] mean and std.dev: (3293.19, 2826.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19138) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 191, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591) [M::mem_pestat] mean and std.dev: (214.01, 112.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 745) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1152, 3238, 5611) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14529) [M::mem_pestat] mean and std.dev: (3700.95, 2882.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18988) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (611, 2345, 5520) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15338) [M::mem_pestat] mean and std.dev: (3291.24, 2959.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20247) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 194.866 CPU sec, 12.506 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (965, 458243, 777, 898) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (654, 2219, 5115) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14037) [M::mem_pestat] mean and std.dev: (3147.33, 2854.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18498) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 191, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591) [M::mem_pestat] mean and std.dev: (214.05, 112.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 745) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1296, 3235, 5672) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14424) [M::mem_pestat] mean and std.dev: (3643.76, 2764.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18800) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (657, 2237, 5407) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14907) [M::mem_pestat] mean and std.dev: (3236.08, 2912.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19657) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 193.280 CPU sec, 12.394 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (952, 458150, 790, 921) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (660, 2298, 5280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14520) [M::mem_pestat] mean and std.dev: (3188.74, 2835.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19140) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 191, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591) [M::mem_pestat] mean and std.dev: (214.02, 112.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 745) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1355, 3204, 5899) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14987) [M::mem_pestat] mean and std.dev: (3739.87, 2829.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19531) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (715, 2485, 5254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14332) [M::mem_pestat] mean and std.dev: (3237.00, 2830.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18871) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 194.181 CPU sec, 12.411 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (942, 458546, 814, 907) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (764, 2521, 5670) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15482) [M::mem_pestat] mean and std.dev: (3409.27, 2956.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20388) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 191, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588) [M::mem_pestat] mean and std.dev: (213.36, 111.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 741) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1109, 2914, 5893) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15461) [M::mem_pestat] mean and std.dev: (3636.99, 2933.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20245) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (821, 2490, 5283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14207) [M::mem_pestat] mean and std.dev: (3274.25, 2832.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18669) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 193.532 CPU sec, 12.465 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (893, 458234, 802, 949) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (764, 2421, 5298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14366) [M::mem_pestat] mean and std.dev: (3281.79, 2884.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18900) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 191, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591) [M::mem_pestat] mean and std.dev: (213.94, 112.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 745) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (975, 2937, 5984) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16002) [M::mem_pestat] mean and std.dev: (3655.77, 2969.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21011) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (771, 2404, 5309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14385) [M::mem_pestat] mean and std.dev: (3322.58, 2899.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18923) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 193.887 CPU sec, 12.389 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (846, 458769, 809, 894) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (709, 2393, 5762) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15868) [M::mem_pestat] mean and std.dev: (3333.61, 2967.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20921) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 191, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594) [M::mem_pestat] mean and std.dev: (214.63, 113.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 749) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1047, 3188, 5884) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15558) [M::mem_pestat] mean and std.dev: (3724.21, 2854.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20395) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (731, 2415, 5700) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15638) [M::mem_pestat] mean and std.dev: (3353.59, 2953.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20607) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1333334 sequences (200000100 bp)... [M::mem_process_seqs] Processed 1333334 reads in 194.981 CPU sec, 12.465 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (862, 458881, 877, 847) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (750, 2542, 5577) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15231) [M::mem_pestat] mean and std.dev: (3399.05, 2929.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20058) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 191, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594) [M::mem_pestat] mean and std.dev: (214.73, 112.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 749) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1225, 3336, 6426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16828) [M::mem_pestat] mean and std.dev: (3924.54, 2977.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 22029) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (707, 2376, 5006) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13604) [M::mem_pestat] mean and std.dev: (3228.34, 2875.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17903) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 194.309 CPU sec, 12.502 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (921, 458658, 821, 993) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (650, 2169, 4767) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13001) [M::mem_pestat] mean and std.dev: (3055.31, 2797.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17118) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 192, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 592) [M::mem_pestat] mean and std.dev: (214.73, 112.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 746) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1207, 3217, 6231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16279) [M::mem_pestat] mean and std.dev: (3767.26, 2845.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21303) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (675, 2144, 4982) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13596) [M::mem_pestat] mean and std.dev: (3083.81, 2781.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17903) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 193.323 CPU sec, 12.670 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (908, 458833, 829, 980) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (696, 2279, 5282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14454) [M::mem_pestat] mean and std.dev: (3176.00, 2856.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19040) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 191, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591) [M::mem_pestat] mean and std.dev: (213.75, 112.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 745) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1207, 2969, 5807) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15007) [M::mem_pestat] mean and std.dev: (3583.75, 2815.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19607) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (717, 2190, 4981) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13509) [M::mem_pestat] mean and std.dev: (3167.17, 2847.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17773) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 192.797 CPU sec, 12.313 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (838, 458671, 837, 926) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (634, 2373, 5246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14470) [M::mem_pestat] mean and std.dev: (3196.57, 2830.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19082) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 190, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588) [M::mem_pestat] mean and std.dev: (213.14, 111.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 741) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1244, 3101, 5971) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15425) [M::mem_pestat] mean and std.dev: (3722.56, 2889.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20152) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (727, 2615, 5845) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16081) [M::mem_pestat] mean and std.dev: (3446.12, 2994.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21199) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 193.271 CPU sec, 12.565 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (886, 458025, 773, 918) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (727, 2529, 5346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14584) [M::mem_pestat] mean and std.dev: (3328.93, 2940.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19203) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 190, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588) [M::mem_pestat] mean and std.dev: (213.20, 111.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 741) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1173, 2861, 5814) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15096) [M::mem_pestat] mean and std.dev: (3635.21, 2865.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19737) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (633, 2465, 5543) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15363) [M::mem_pestat] mean and std.dev: (3258.42, 2911.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20273) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 194.566 CPU sec, 13.061 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (888, 459270, 771, 929) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (851, 2512, 5502) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14804) [M::mem_pestat] mean and std.dev: (3295.70, 2786.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19455) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 190, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 585) [M::mem_pestat] mean and std.dev: (212.66, 111.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 737) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1092, 2917, 5856) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15384) [M::mem_pestat] mean and std.dev: (3553.01, 2821.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20148) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (699, 2290, 5484) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15054) [M::mem_pestat] mean and std.dev: (3278.94, 2954.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19839) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 195.137 CPU sec, 12.677 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (938, 457945, 816, 929) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (612, 2227, 5118) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14130) [M::mem_pestat] mean and std.dev: (3203.56, 2982.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18636) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 190, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 585) [M::mem_pestat] mean and std.dev: (212.79, 111.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 737) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1321, 3582, 6569) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 17065) [M::mem_pestat] mean and std.dev: (4052.19, 3014.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 22313) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (765, 2628, 5526) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15048) [M::mem_pestat] mean and std.dev: (3397.79, 2881.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19809) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 194.595 CPU sec, 13.068 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (877, 458262, 812, 879) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (636, 2291, 5361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14811) [M::mem_pestat] mean and std.dev: (3239.97, 2916.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19536) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 190, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 585) [M::mem_pestat] mean and std.dev: (212.83, 111.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 737) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1155, 3367, 6166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16188) [M::mem_pestat] mean and std.dev: (3767.75, 2855.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21199) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (666, 2242, 5118) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14022) [M::mem_pestat] mean and std.dev: (3137.27, 2859.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18474) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 195.095 CPU sec, 12.691 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (937, 458311, 763, 836) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (752, 2608, 5319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14453) [M::mem_pestat] mean and std.dev: (3273.67, 2812.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19020) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 190, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 585) [M::mem_pestat] mean and std.dev: (212.82, 111.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 737) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (998, 2858, 5690) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15074) [M::mem_pestat] mean and std.dev: (3577.90, 2917.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19766) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (801, 2498, 6100) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16698) [M::mem_pestat] mean and std.dev: (3536.43, 3073.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21997) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 195.664 CPU sec, 12.555 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (950, 458222, 740, 868) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (725, 2455, 5527) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15131) [M::mem_pestat] mean and std.dev: (3334.53, 2925.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19933) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 190, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 585) [M::mem_pestat] mean and std.dev: (212.46, 111.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 737) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1023, 2998, 6196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16542) [M::mem_pestat] mean and std.dev: (3686.73, 2955.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21715) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (756, 2643, 5493) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14967) [M::mem_pestat] mean and std.dev: (3385.08, 2904.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19704) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 196.098 CPU sec, 12.743 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (903, 459067, 738, 894) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (771, 2654, 5544) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15090) [M::mem_pestat] mean and std.dev: (3354.47, 2852.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19863) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 189, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 584) [M::mem_pestat] mean and std.dev: (211.92, 111.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 736) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1380, 3336, 6202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15846) [M::mem_pestat] mean and std.dev: (3946.29, 2910.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20668) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (805, 2641, 5337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14401) [M::mem_pestat] mean and std.dev: (3340.31, 2801.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18933) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 193.779 CPU sec, 12.892 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (923, 459505, 755, 886) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (727, 2577, 5609) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15373) [M::mem_pestat] mean and std.dev: (3334.87, 2796.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20255) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 189, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 581) [M::mem_pestat] mean and std.dev: (211.38, 110.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 732) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1166, 3099, 5948) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15512) [M::mem_pestat] mean and std.dev: (3774.37, 2929.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20294) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (741, 2200, 5325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14493) [M::mem_pestat] mean and std.dev: (3191.24, 2835.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19077) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 193.306 CPU sec, 12.550 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (889, 458468, 770, 911) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (659, 2407, 5284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14534) [M::mem_pestat] mean and std.dev: (3229.85, 2876.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19159) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 188, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 578) [M::mem_pestat] mean and std.dev: (210.37, 109.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 728) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1170, 3114, 5623) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14529) [M::mem_pestat] mean and std.dev: (3616.71, 2819.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18982) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (788, 2633, 5446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14762) [M::mem_pestat] mean and std.dev: (3373.68, 2895.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19420) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 195.082 CPU sec, 12.864 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (874, 458263, 778, 838) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (701, 2433, 5470) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15008) [M::mem_pestat] mean and std.dev: (3333.19, 2960.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19777) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (127, 187, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 574) [M::mem_pestat] mean and std.dev: (209.21, 108.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 723) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1180, 3033, 5752) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14896) [M::mem_pestat] mean and std.dev: (3674.79, 2860.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19468) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (748, 2609, 5840) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16024) [M::mem_pestat] mean and std.dev: (3471.73, 2941.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21116) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 194.695 CPU sec, 12.641 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (871, 457829, 776, 828) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (626, 2341, 5212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14384) [M::mem_pestat] mean and std.dev: (3215.68, 2867.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18970) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (127, 188, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 580) [M::mem_pestat] mean and std.dev: (210.21, 109.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 731) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1036, 3137, 5955) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15793) [M::mem_pestat] mean and std.dev: (3709.95, 2891.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20712) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (647, 2257, 5362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14792) [M::mem_pestat] mean and std.dev: (3230.28, 2921.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19507) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 192.691 CPU sec, 12.576 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (896, 458375, 792, 831) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (736, 2346, 5109) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13855) [M::mem_pestat] mean and std.dev: (3204.54, 2816.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18228) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (127, 187, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 580) [M::mem_pestat] mean and std.dev: (210.00, 109.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 731) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1081, 3259, 6118) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16192) [M::mem_pestat] mean and std.dev: (3784.38, 2955.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21229) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (766, 2414, 5463) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14857) [M::mem_pestat] mean and std.dev: (3308.49, 2895.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19554) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 193.916 CPU sec, 12.586 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (898, 458474, 819, 855) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (705, 2911, 5583) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15339) [M::mem_pestat] mean and std.dev: (3456.53, 2954.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20217) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (127, 188, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 580) [M::mem_pestat] mean and std.dev: (210.13, 109.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 731) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1217, 3099, 5921) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15329) [M::mem_pestat] mean and std.dev: (3742.16, 2904.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20033) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (659, 2569, 5502) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15188) [M::mem_pestat] mean and std.dev: (3309.41, 2909.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20031) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 195.050 CPU sec, 13.258 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (938, 458130, 761, 823) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (680, 2287, 5265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14435) [M::mem_pestat] mean and std.dev: (3182.41, 2850.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19020) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 189, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 587) [M::mem_pestat] mean and std.dev: (212.31, 111.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 740) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1137, 3219, 6082) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15972) [M::mem_pestat] mean and std.dev: (3765.44, 2878.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20917) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (752, 2530, 5499) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14993) [M::mem_pestat] mean and std.dev: (3324.65, 2833.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19740) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 195.038 CPU sec, 12.689 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (936, 457657, 828, 913) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (729, 2409, 5112) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13878) [M::mem_pestat] mean and std.dev: (3160.37, 2748.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18261) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 191, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591) [M::mem_pestat] mean and std.dev: (214.26, 112.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 745) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1144, 3065, 6119) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16069) [M::mem_pestat] mean and std.dev: (3737.31, 2930.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21044) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (753, 2347, 5291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14367) [M::mem_pestat] mean and std.dev: (3291.42, 2859.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18905) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 195.700 CPU sec, 12.437 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (896, 457427, 807, 935) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (717, 2473, 5487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15027) [M::mem_pestat] mean and std.dev: (3325.04, 2934.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19797) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 190, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588) [M::mem_pestat] mean and std.dev: (213.58, 112.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 741) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1035, 3327, 5857) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15501) [M::mem_pestat] mean and std.dev: (3762.42, 2931.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20323) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (731, 2402, 5434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14840) [M::mem_pestat] mean and std.dev: (3262.66, 2867.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19543) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 196.266 CPU sec, 12.740 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (943, 456751, 796, 905) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (784, 2669, 5680) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15472) [M::mem_pestat] mean and std.dev: (3407.25, 2888.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20368) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 191, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591) [M::mem_pestat] mean and std.dev: (213.76, 112.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 745) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1091, 3058, 5960) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15698) [M::mem_pestat] mean and std.dev: (3688.56, 2918.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20567) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (903, 2612, 5790) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15564) [M::mem_pestat] mean and std.dev: (3477.03, 2902.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20451) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 195.105 CPU sec, 13.341 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (923, 457720, 731, 960) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (799, 2458, 5077) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13633) [M::mem_pestat] mean and std.dev: (3236.54, 2795.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17911) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 191, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591) [M::mem_pestat] mean and std.dev: (213.90, 112.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 745) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1091, 3163, 6207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16439) [M::mem_pestat] mean and std.dev: (3779.38, 3008.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21555) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (759, 2478, 5811) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15915) [M::mem_pestat] mean and std.dev: (3346.69, 2889.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20967) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 194.336 CPU sec, 12.650 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (877, 458453, 745, 910) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (734, 2656, 5616) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15380) [M::mem_pestat] mean and std.dev: (3446.35, 2995.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20262) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 191, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588) [M::mem_pestat] mean and std.dev: (213.54, 111.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 741) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1231, 3160, 6024) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15610) [M::mem_pestat] mean and std.dev: (3810.48, 2932.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20403) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (690, 2437, 5518) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15174) [M::mem_pestat] mean and std.dev: (3293.16, 2898.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20002) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 193.865 CPU sec, 12.348 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (887, 458031, 810, 975) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (847, 2599, 5674) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15328) [M::mem_pestat] mean and std.dev: (3522.77, 2995.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20155) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 191, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591) [M::mem_pestat] mean and std.dev: (213.89, 112.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 745) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (820, 3030, 5421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14623) [M::mem_pestat] mean and std.dev: (3493.67, 2851.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19224) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (891, 2469, 5679) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15255) [M::mem_pestat] mean and std.dev: (3375.59, 2888.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20043) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 194.785 CPU sec, 12.605 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (863, 458161, 782, 891) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (729, 2397, 5294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14424) [M::mem_pestat] mean and std.dev: (3224.08, 2831.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18989) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 191, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591) [M::mem_pestat] mean and std.dev: (213.83, 112.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 745) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1150, 3281, 6094) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15982) [M::mem_pestat] mean and std.dev: (3829.59, 2871.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20926) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (740, 2394, 5585) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15275) [M::mem_pestat] mean and std.dev: (3349.82, 2906.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20120) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 193.569 CPU sec, 13.010 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (875, 458533, 775, 940) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (744, 2484, 5619) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15369) [M::mem_pestat] mean and std.dev: (3342.77, 2913.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20244) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 191, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588) [M::mem_pestat] mean and std.dev: (213.78, 112.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 741) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1316, 3356, 6022) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15434) [M::mem_pestat] mean and std.dev: (3881.60, 2929.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20140) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (615, 2253, 5192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14346) [M::mem_pestat] mean and std.dev: (3168.80, 2848.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18923) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 193.942 CPU sec, 12.608 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (952, 458790, 777, 889) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (875, 2823, 5688) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15314) [M::mem_pestat] mean and std.dev: (3513.08, 2896.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20127) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 191, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591) [M::mem_pestat] mean and std.dev: (214.16, 112.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 745) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1368, 3382, 5999) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15261) [M::mem_pestat] mean and std.dev: (3854.49, 2856.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19892) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (719, 2441, 5329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14549) [M::mem_pestat] mean and std.dev: (3295.27, 2865.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19159) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 193.724 CPU sec, 12.467 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (953, 457761, 776, 903) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (757, 2770, 5386) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14644) [M::mem_pestat] mean and std.dev: (3401.68, 2865.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19273) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 191, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591) [M::mem_pestat] mean and std.dev: (214.04, 112.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 745) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1242, 3193, 6209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16143) [M::mem_pestat] mean and std.dev: (3771.47, 2880.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21110) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (839, 2772, 5696) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15410) [M::mem_pestat] mean and std.dev: (3471.04, 2900.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20267) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 196.736 CPU sec, 12.735 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (936, 457766, 803, 931) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (771, 2800, 5999) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16455) [M::mem_pestat] mean and std.dev: (3460.88, 2952.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21683) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 192, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595) [M::mem_pestat] mean and std.dev: (215.27, 113.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 750) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1140, 3249, 6138) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16134) [M::mem_pestat] mean and std.dev: (3736.63, 2866.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21132) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (784, 2303, 5156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13900) [M::mem_pestat] mean and std.dev: (3262.73, 2911.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18272) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 195.007 CPU sec, 12.444 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (960, 458796, 786, 850) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (649, 2412, 5296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14590) [M::mem_pestat] mean and std.dev: (3230.89, 2843.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19237) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 192, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594) [M::mem_pestat] mean and std.dev: (214.76, 113.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 749) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1353, 3481, 6569) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 17001) [M::mem_pestat] mean and std.dev: (3990.34, 2936.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 22217) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (891, 2705, 5825) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15693) [M::mem_pestat] mean and std.dev: (3490.45, 2926.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20627) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 195.469 CPU sec, 12.705 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (912, 458585, 763, 867) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (727, 2317, 5270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14356) [M::mem_pestat] mean and std.dev: (3236.60, 2902.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18899) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 191, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594) [M::mem_pestat] mean and std.dev: (214.14, 113.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 749) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1266, 3181, 6031) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15561) [M::mem_pestat] mean and std.dev: (3772.72, 2876.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20326) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (604, 2327, 5238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14506) [M::mem_pestat] mean and std.dev: (3196.97, 2857.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19140) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 194.226 CPU sec, 12.890 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (974, 458597, 787, 843) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (807, 2587, 5750) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15636) [M::mem_pestat] mean and std.dev: (3409.46, 2900.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20579) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 191, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591) [M::mem_pestat] mean and std.dev: (213.89, 112.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 745) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1179, 3040, 5915) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15387) [M::mem_pestat] mean and std.dev: (3686.88, 2827.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20123) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (751, 2249, 5368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14602) [M::mem_pestat] mean and std.dev: (3200.98, 2823.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19219) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 195.642 CPU sec, 12.730 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (894, 458320, 812, 873) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (728, 2505, 5334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14546) [M::mem_pestat] mean and std.dev: (3269.67, 2858.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19152) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 191, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588) [M::mem_pestat] mean and std.dev: (213.75, 112.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 741) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1444, 3293, 6025) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15187) [M::mem_pestat] mean and std.dev: (3870.55, 2877.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19768) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (695, 2104, 5051) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13763) [M::mem_pestat] mean and std.dev: (3130.24, 2809.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18119) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 193.479 CPU sec, 12.920 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (977, 458262, 752, 845) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (671, 2474, 5340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14678) [M::mem_pestat] mean and std.dev: (3254.21, 2897.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19347) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 191, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588) [M::mem_pestat] mean and std.dev: (213.52, 112.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 741) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1189, 3487, 6540) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 17242) [M::mem_pestat] mean and std.dev: (3914.51, 2955.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 22593) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (693, 2498, 5463) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15003) [M::mem_pestat] mean and std.dev: (3315.29, 2916.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19773) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 194.245 CPU sec, 12.620 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (916, 458047, 819, 900) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (774, 2201, 5681) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15495) [M::mem_pestat] mean and std.dev: (3350.22, 2974.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20402) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 191, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588) [M::mem_pestat] mean and std.dev: (213.28, 111.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 741) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1004, 3088, 5657) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14963) [M::mem_pestat] mean and std.dev: (3606.87, 2901.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19616) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (661, 2330, 5295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14563) [M::mem_pestat] mean and std.dev: (3235.25, 2903.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19197) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 193.352 CPU sec, 12.764 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (905, 458068, 756, 935) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (573, 2477, 5398) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15048) [M::mem_pestat] mean and std.dev: (3262.42, 2878.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19873) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 190, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 585) [M::mem_pestat] mean and std.dev: (212.76, 111.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 737) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1369, 3308, 6156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15730) [M::mem_pestat] mean and std.dev: (3874.02, 2840.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20517) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (728, 2225, 5011) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13577) [M::mem_pestat] mean and std.dev: (3104.30, 2762.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17860) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 194.261 CPU sec, 12.622 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (937, 459185, 818, 834) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (604, 2274, 5353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14851) [M::mem_pestat] mean and std.dev: (3285.52, 2958.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19600) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 190, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588) [M::mem_pestat] mean and std.dev: (213.42, 112.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 741) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1227, 3275, 6110) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15876) [M::mem_pestat] mean and std.dev: (3804.79, 2889.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20759) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (719, 2516, 5455) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14927) [M::mem_pestat] mean and std.dev: (3280.56, 2818.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19663) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 194.657 CPU sec, 13.038 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (948, 458007, 782, 862) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (659, 2441, 5535) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15287) [M::mem_pestat] mean and std.dev: (3328.40, 2955.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20163) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 190, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588) [M::mem_pestat] mean and std.dev: (213.19, 111.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 741) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1264, 3084, 5972) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15388) [M::mem_pestat] mean and std.dev: (3758.13, 2885.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20096) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (765, 2565, 5577) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15201) [M::mem_pestat] mean and std.dev: (3403.38, 2911.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20013) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 194.015 CPU sec, 12.594 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (898, 459195, 762, 817) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (719, 2382, 5731) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15755) [M::mem_pestat] mean and std.dev: (3382.54, 2988.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20767) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 190, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588) [M::mem_pestat] mean and std.dev: (213.26, 111.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 741) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1269, 3171, 5704) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14574) [M::mem_pestat] mean and std.dev: (3695.70, 2828.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19009) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (802, 2726, 5699) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15493) [M::mem_pestat] mean and std.dev: (3429.50, 2981.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20390) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 194.327 CPU sec, 12.983 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (924, 458312, 783, 887) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (676, 2222, 5670) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15658) [M::mem_pestat] mean and std.dev: (3297.26, 2928.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20652) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 190, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 587) [M::mem_pestat] mean and std.dev: (212.61, 111.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 740) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1197, 3147, 5896) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15294) [M::mem_pestat] mean and std.dev: (3738.30, 2886.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19993) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (823, 2304, 5655) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15319) [M::mem_pestat] mean and std.dev: (3326.35, 2939.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20151) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 195.091 CPU sec, 12.686 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (885, 457547, 766, 886) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (617, 2255, 5160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14246) [M::mem_pestat] mean and std.dev: (3170.12, 2854.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18789) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 189, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 584) [M::mem_pestat] mean and std.dev: (211.43, 110.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 736) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1214, 3264, 6136) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15980) [M::mem_pestat] mean and std.dev: (3803.69, 2864.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20902) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (718, 2390, 5466) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14962) [M::mem_pestat] mean and std.dev: (3298.22, 2918.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19710) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 197.006 CPU sec, 13.097 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (867, 459129, 757, 921) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (724, 2301, 5263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14341) [M::mem_pestat] mean and std.dev: (3202.13, 2809.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18880) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 188, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 581) [M::mem_pestat] mean and std.dev: (210.81, 110.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 732) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1158, 3257, 5975) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15609) [M::mem_pestat] mean and std.dev: (3763.00, 2923.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20426) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (726, 2590, 5663) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15537) [M::mem_pestat] mean and std.dev: (3395.23, 2972.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20474) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 195.353 CPU sec, 12.637 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (843, 457671, 791, 820) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (737, 2556, 5157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13997) [M::mem_pestat] mean and std.dev: (3236.71, 2796.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18417) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 188, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 581) [M::mem_pestat] mean and std.dev: (210.78, 110.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 732) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1331, 3132, 6135) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15743) [M::mem_pestat] mean and std.dev: (3803.20, 2909.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20547) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (801, 2620, 5173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13917) [M::mem_pestat] mean and std.dev: (3350.02, 2851.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18289) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 195.734 CPU sec, 12.503 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (850, 457840, 773, 838) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (714, 2440, 5407) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14793) [M::mem_pestat] mean and std.dev: (3270.32, 2866.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19486) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 188, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 581) [M::mem_pestat] mean and std.dev: (210.67, 110.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 732) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1350, 3483, 6080) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15540) [M::mem_pestat] mean and std.dev: (3879.35, 2781.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20270) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (579, 2411, 5410) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15072) [M::mem_pestat] mean and std.dev: (3268.55, 2938.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19903) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 194.368 CPU sec, 12.623 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (893, 457968, 775, 922) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (810, 2549, 5813) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15819) [M::mem_pestat] mean and std.dev: (3447.72, 2947.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20822) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (127, 188, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 583) [M::mem_pestat] mean and std.dev: (210.91, 110.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 735) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1067, 3091, 6125) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16241) [M::mem_pestat] mean and std.dev: (3755.35, 2956.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21299) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (712, 2497, 5211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14209) [M::mem_pestat] mean and std.dev: (3256.36, 2816.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18708) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 192.624 CPU sec, 12.852 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (918, 458290, 806, 949) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (595, 2110, 5109) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14137) [M::mem_pestat] mean and std.dev: (3126.90, 2901.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18651) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 190, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 587) [M::mem_pestat] mean and std.dev: (212.62, 111.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 740) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1102, 2868, 5741) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15019) [M::mem_pestat] mean and std.dev: (3598.62, 2894.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19658) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (734, 2620, 5522) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15098) [M::mem_pestat] mean and std.dev: (3374.49, 2949.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19886) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 192.742 CPU sec, 12.501 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (958, 458395, 775, 878) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (710, 2608, 5428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14864) [M::mem_pestat] mean and std.dev: (3278.81, 2822.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19582) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 191, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594) [M::mem_pestat] mean and std.dev: (214.93, 113.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 749) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (973, 2806, 5638) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14968) [M::mem_pestat] mean and std.dev: (3535.67, 2908.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19633) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (725, 2518, 5726) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15728) [M::mem_pestat] mean and std.dev: (3361.31, 2977.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20729) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 194.379 CPU sec, 12.759 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (903, 457696, 797, 900) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (742, 2592, 5396) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14704) [M::mem_pestat] mean and std.dev: (3331.38, 2911.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19358) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 191, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591) [M::mem_pestat] mean and std.dev: (214.26, 112.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 745) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1090, 3026, 5928) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15604) [M::mem_pestat] mean and std.dev: (3675.23, 2909.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20442) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (831, 2567, 5515) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14883) [M::mem_pestat] mean and std.dev: (3374.99, 2890.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19567) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 196.451 CPU sec, 12.713 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (921, 457987, 779, 895) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (614, 2408, 5613) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15611) [M::mem_pestat] mean and std.dev: (3247.69, 2902.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20610) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 191, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591) [M::mem_pestat] mean and std.dev: (214.11, 112.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 745) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1243, 3211, 6093) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15793) [M::mem_pestat] mean and std.dev: (3739.82, 2813.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20643) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (801, 2347, 5178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13932) [M::mem_pestat] mean and std.dev: (3221.38, 2776.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18309) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 194.598 CPU sec, 12.632 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (918, 457647, 809, 919) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (603, 2120, 5185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14349) [M::mem_pestat] mean and std.dev: (3135.28, 2856.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18931) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 191, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594) [M::mem_pestat] mean and std.dev: (214.54, 112.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 749) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1334, 3259, 5861) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14915) [M::mem_pestat] mean and std.dev: (3816.82, 2854.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19442) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (873, 2571, 5429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14541) [M::mem_pestat] mean and std.dev: (3393.65, 2896.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19097) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 197.524 CPU sec, 12.873 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (962, 457626, 779, 946) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (728, 2396, 5514) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15086) [M::mem_pestat] mean and std.dev: (3314.88, 2909.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19872) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 191, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591) [M::mem_pestat] mean and std.dev: (213.78, 112.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 745) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1263, 3035, 6113) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15813) [M::mem_pestat] mean and std.dev: (3694.08, 2866.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20663) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (787, 2530, 5379) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14563) [M::mem_pestat] mean and std.dev: (3342.26, 2893.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19155) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 193.118 CPU sec, 12.919 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (949, 458069, 752, 917) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (702, 2293, 5473) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15015) [M::mem_pestat] mean and std.dev: (3249.26, 2896.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19786) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 190, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588) [M::mem_pestat] mean and std.dev: (213.53, 112.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 741) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1194, 3154, 5925) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15387) [M::mem_pestat] mean and std.dev: (3699.43, 2866.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20118) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (757, 2584, 5667) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15487) [M::mem_pestat] mean and std.dev: (3396.37, 2936.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20397) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 193.773 CPU sec, 12.617 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (884, 458390, 802, 915) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (720, 2601, 5374) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14682) [M::mem_pestat] mean and std.dev: (3317.97, 2867.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19336) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 191, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588) [M::mem_pestat] mean and std.dev: (213.66, 112.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 741) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1223, 3229, 6257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16325) [M::mem_pestat] mean and std.dev: (3829.67, 2941.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21359) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (727, 2243, 5430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14836) [M::mem_pestat] mean and std.dev: (3243.99, 2928.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19539) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 194.295 CPU sec, 12.598 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (927, 458247, 754, 947) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (725, 2223, 5207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14171) [M::mem_pestat] mean and std.dev: (3199.94, 2876.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18653) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 191, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594) [M::mem_pestat] mean and std.dev: (214.59, 112.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 749) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1390, 3294, 5665) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14215) [M::mem_pestat] mean and std.dev: (3756.82, 2758.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18490) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (702, 2345, 5576) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15324) [M::mem_pestat] mean and std.dev: (3274.30, 2915.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20198) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 195.012 CPU sec, 12.698 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (868, 458013, 819, 945) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (678, 2268, 5424) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14916) [M::mem_pestat] mean and std.dev: (3212.37, 2911.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19662) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 191, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591) [M::mem_pestat] mean and std.dev: (214.09, 112.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 745) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1145, 3253, 5978) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15644) [M::mem_pestat] mean and std.dev: (3793.20, 2924.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20477) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (822, 2428, 5242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14082) [M::mem_pestat] mean and std.dev: (3294.28, 2855.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18502) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 195.770 CPU sec, 12.521 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (922, 458347, 816, 848) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (684, 2414, 5220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14292) [M::mem_pestat] mean and std.dev: (3285.42, 2927.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18828) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 192, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594) [M::mem_pestat] mean and std.dev: (214.90, 113.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 749) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1296, 3024, 6034) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15510) [M::mem_pestat] mean and std.dev: (3679.45, 2858.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20248) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (857, 2731, 5612) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15122) [M::mem_pestat] mean and std.dev: (3435.09, 2899.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19877) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 196.299 CPU sec, 12.569 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (944, 458469, 770, 948) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (609, 2491, 5112) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14118) [M::mem_pestat] mean and std.dev: (3208.69, 2801.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18621) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 192, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595) [M::mem_pestat] mean and std.dev: (215.23, 113.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 750) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1120, 3209, 5780) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15100) [M::mem_pestat] mean and std.dev: (3718.83, 2793.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19760) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (815, 2328, 5208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13994) [M::mem_pestat] mean and std.dev: (3258.30, 2846.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18387) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 195.652 CPU sec, 12.696 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (892, 458105, 822, 839) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (698, 2735, 6310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 17534) [M::mem_pestat] mean and std.dev: (3632.36, 3138.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 23146) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 192, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595) [M::mem_pestat] mean and std.dev: (215.58, 113.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 750) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1217, 3204, 5891) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15239) [M::mem_pestat] mean and std.dev: (3758.12, 2873.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19913) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (730, 2440, 5380) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14680) [M::mem_pestat] mean and std.dev: (3267.52, 2817.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19330) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 193.787 CPU sec, 12.679 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (981, 458134, 782, 820) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (632, 2050, 5032) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13832) [M::mem_pestat] mean and std.dev: (2996.17, 2766.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18232) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 192, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594) [M::mem_pestat] mean and std.dev: (214.78, 112.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 749) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1145, 2973, 5909) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15437) [M::mem_pestat] mean and std.dev: (3646.34, 2856.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20201) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (699, 2427, 5504) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15114) [M::mem_pestat] mean and std.dev: (3303.60, 2870.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19919) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 193.454 CPU sec, 12.582 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (849, 458677, 841, 945) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (623, 2386, 5150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14204) [M::mem_pestat] mean and std.dev: (3185.30, 2799.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18731) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 192, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594) [M::mem_pestat] mean and std.dev: (214.81, 113.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 749) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1105, 3116, 6171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16303) [M::mem_pestat] mean and std.dev: (3761.38, 2923.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21369) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (757, 2462, 5587) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15247) [M::mem_pestat] mean and std.dev: (3338.91, 2896.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20077) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 194.055 CPU sec, 12.401 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (887, 458065, 727, 889) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (702, 2349, 5572) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15312) [M::mem_pestat] mean and std.dev: (3322.53, 2958.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20182) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 191, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588) [M::mem_pestat] mean and std.dev: (213.50, 111.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 741) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1126, 3178, 5655) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14713) [M::mem_pestat] mean and std.dev: (3700.09, 2827.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19242) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (704, 2201, 5696) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15680) [M::mem_pestat] mean and std.dev: (3270.06, 2931.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20672) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 195.036 CPU sec, 12.784 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (944, 457986, 808, 913) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (621, 2357, 5614) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15600) [M::mem_pestat] mean and std.dev: (3284.63, 2960.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20593) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 192, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 592) [M::mem_pestat] mean and std.dev: (214.64, 112.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 746) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1133, 3129, 6316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16682) [M::mem_pestat] mean and std.dev: (3836.09, 3029.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21865) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (733, 2592, 5765) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15829) [M::mem_pestat] mean and std.dev: (3432.41, 2959.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20861) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 194.499 CPU sec, 12.520 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (894, 458165, 782, 938) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (630, 2413, 5696) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15828) [M::mem_pestat] mean and std.dev: (3312.20, 2946.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20894) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 192, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594) [M::mem_pestat] mean and std.dev: (215.01, 113.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 749) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1186, 3062, 5939) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15445) [M::mem_pestat] mean and std.dev: (3671.24, 2823.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20198) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (690, 2566, 5717) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15771) [M::mem_pestat] mean and std.dev: (3380.34, 2993.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20798) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 195.319 CPU sec, 12.747 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (915, 457403, 745, 905) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (788, 2343, 5354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14486) [M::mem_pestat] mean and std.dev: (3275.67, 2875.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19052) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 191, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591) [M::mem_pestat] mean and std.dev: (214.01, 112.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 745) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1163, 2968, 5984) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15626) [M::mem_pestat] mean and std.dev: (3738.00, 2930.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20447) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (830, 2541, 5538) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14954) [M::mem_pestat] mean and std.dev: (3407.60, 2889.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19662) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 195.814 CPU sec, 12.856 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (906, 458253, 801, 964) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (839, 2614, 5773) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15641) [M::mem_pestat] mean and std.dev: (3449.41, 2925.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20575) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 192, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594) [M::mem_pestat] mean and std.dev: (214.65, 112.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 749) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1055, 3104, 6036) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15998) [M::mem_pestat] mean and std.dev: (3711.94, 2926.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20979) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (745, 2242, 5355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14575) [M::mem_pestat] mean and std.dev: (3172.32, 2827.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19185) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 195.330 CPU sec, 12.874 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (934, 458431, 856, 909) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (735, 2559, 5529) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15117) [M::mem_pestat] mean and std.dev: (3359.83, 2898.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19911) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 191, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594) [M::mem_pestat] mean and std.dev: (214.68, 113.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 749) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1086, 3334, 6077) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16059) [M::mem_pestat] mean and std.dev: (3781.67, 2882.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21050) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (744, 2394, 5586) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15270) [M::mem_pestat] mean and std.dev: (3271.33, 2864.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20112) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 193.947 CPU sec, 12.534 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (913, 457942, 830, 900) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (608, 2150, 4951) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13637) [M::mem_pestat] mean and std.dev: (3129.12, 2896.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17980) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 191, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591) [M::mem_pestat] mean and std.dev: (213.90, 112.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 745) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1425, 3328, 5972) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15066) [M::mem_pestat] mean and std.dev: (3828.26, 2866.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19613) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (649, 2075, 5305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14617) [M::mem_pestat] mean and std.dev: (3083.11, 2799.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19273) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 193.938 CPU sec, 12.591 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (869, 457739, 782, 892) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (637, 2335, 5278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14560) [M::mem_pestat] mean and std.dev: (3170.26, 2847.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19201) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 190, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588) [M::mem_pestat] mean and std.dev: (213.01, 111.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 741) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1238, 3123, 5704) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14636) [M::mem_pestat] mean and std.dev: (3725.29, 2837.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19102) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (800, 2548, 5854) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15962) [M::mem_pestat] mean and std.dev: (3444.38, 2957.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21016) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 194.065 CPU sec, 12.540 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (873, 458366, 749, 917) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (666, 2405, 5505) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15183) [M::mem_pestat] mean and std.dev: (3285.75, 2879.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20022) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 189, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 584) [M::mem_pestat] mean and std.dev: (211.95, 110.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 736) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1114, 3131, 5941) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15595) [M::mem_pestat] mean and std.dev: (3676.85, 2924.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20422) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (647, 2258, 5189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14273) [M::mem_pestat] mean and std.dev: (3184.81, 2879.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18815) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 195.043 CPU sec, 12.725 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (951, 457630, 750, 915) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (604, 2388, 5056) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13960) [M::mem_pestat] mean and std.dev: (3092.65, 2782.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18412) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 189, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 581) [M::mem_pestat] mean and std.dev: (210.97, 110.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 732) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1040, 3070, 6119) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16277) [M::mem_pestat] mean and std.dev: (3695.16, 2946.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21356) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (731, 2291, 5193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14117) [M::mem_pestat] mean and std.dev: (3184.83, 2851.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18579) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 195.394 CPU sec, 12.408 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (906, 458576, 795, 876) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (701, 2296, 5664) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15590) [M::mem_pestat] mean and std.dev: (3288.45, 2979.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20553) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 189, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 581) [M::mem_pestat] mean and std.dev: (210.95, 110.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 732) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1264, 3139, 5967) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15373) [M::mem_pestat] mean and std.dev: (3737.03, 2821.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20076) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (664, 2497, 5175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14197) [M::mem_pestat] mean and std.dev: (3276.26, 2916.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18708) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 193.072 CPU sec, 12.537 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (949, 458692, 737, 901) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (732, 2641, 5705) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15651) [M::mem_pestat] mean and std.dev: (3400.64, 2965.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20624) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 188, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 581) [M::mem_pestat] mean and std.dev: (210.80, 110.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 732) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1270, 3088, 5683) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14509) [M::mem_pestat] mean and std.dev: (3697.18, 2838.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18922) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (677, 2473, 5726) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15824) [M::mem_pestat] mean and std.dev: (3330.71, 2917.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20873) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 193.638 CPU sec, 12.510 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (919, 457120, 824, 925) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (618, 2285, 5655) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15729) [M::mem_pestat] mean and std.dev: (3287.84, 3003.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20766) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 189, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 581) [M::mem_pestat] mean and std.dev: (211.19, 110.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 732) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1254, 3270, 6018) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15546) [M::mem_pestat] mean and std.dev: (3797.22, 2862.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20310) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (758, 2496, 5654) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15446) [M::mem_pestat] mean and std.dev: (3419.83, 2947.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20342) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 194.451 CPU sec, 12.620 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (940, 458302, 791, 854) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (591, 2271, 5674) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15840) [M::mem_pestat] mean and std.dev: (3314.62, 3003.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20923) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 191, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591) [M::mem_pestat] mean and std.dev: (213.75, 112.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 745) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1265, 3518, 6811) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 17903) [M::mem_pestat] mean and std.dev: (4132.38, 3104.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 23449) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (709, 2579, 5104) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13894) [M::mem_pestat] mean and std.dev: (3231.88, 2804.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18289) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 193.512 CPU sec, 12.494 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (853, 457842, 787, 867) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (696, 2398, 5533) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15207) [M::mem_pestat] mean and std.dev: (3282.76, 2881.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20044) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 191, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594) [M::mem_pestat] mean and std.dev: (214.95, 113.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 749) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1192, 2983, 6183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16165) [M::mem_pestat] mean and std.dev: (3782.87, 2908.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21156) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (752, 2573, 5276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14324) [M::mem_pestat] mean and std.dev: (3264.53, 2829.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18848) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 196.511 CPU sec, 12.887 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (921, 458294, 833, 914) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (722, 2647, 5530) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15146) [M::mem_pestat] mean and std.dev: (3360.27, 2882.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19954) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 191, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591) [M::mem_pestat] mean and std.dev: (214.27, 112.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 745) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1041, 3073, 6201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16521) [M::mem_pestat] mean and std.dev: (3748.16, 2999.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21681) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (670, 2066, 5154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14122) [M::mem_pestat] mean and std.dev: (3077.52, 2849.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18606) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 195.934 CPU sec, 12.457 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (879, 457596, 766, 879) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (702, 2369, 5140) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14016) [M::mem_pestat] mean and std.dev: (3197.96, 2856.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18454) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 191, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588) [M::mem_pestat] mean and std.dev: (213.64, 112.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 741) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1267, 3485, 5945) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15301) [M::mem_pestat] mean and std.dev: (3830.39, 2859.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19979) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (643, 2589, 5398) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14908) [M::mem_pestat] mean and std.dev: (3304.11, 2888.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19663) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 198.703 CPU sec, 13.117 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (897, 458226, 779, 920) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (630, 2751, 5746) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15978) [M::mem_pestat] mean and std.dev: (3440.48, 2984.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21094) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 191, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591) [M::mem_pestat] mean and std.dev: (213.78, 112.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 745) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1112, 3131, 5987) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15737) [M::mem_pestat] mean and std.dev: (3745.59, 2913.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20612) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (862, 2673, 5716) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15424) [M::mem_pestat] mean and std.dev: (3459.56, 2913.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20278) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 194.105 CPU sec, 12.341 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (951, 458300, 749, 874) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (753, 2584, 5644) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15426) [M::mem_pestat] mean and std.dev: (3371.07, 2912.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20317) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 191, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591) [M::mem_pestat] mean and std.dev: (214.13, 112.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 745) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1222, 2884, 5715) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14701) [M::mem_pestat] mean and std.dev: (3649.37, 2843.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19194) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (746, 2536, 5454) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14870) [M::mem_pestat] mean and std.dev: (3336.08, 2835.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19578) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 193.481 CPU sec, 12.515 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (940, 458257, 795, 869) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (733, 2371, 5300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14434) [M::mem_pestat] mean and std.dev: (3265.12, 2898.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19001) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 191, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594) [M::mem_pestat] mean and std.dev: (214.65, 113.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 749) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1171, 2961, 5645) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14593) [M::mem_pestat] mean and std.dev: (3647.01, 2826.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19067) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (718, 2464, 5379) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14701) [M::mem_pestat] mean and std.dev: (3308.85, 2890.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19362) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 191.821 CPU sec, 12.203 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1002, 457802, 821, 942) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (680, 2311, 5166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14138) [M::mem_pestat] mean and std.dev: (3217.42, 2878.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18624) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 191, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591) [M::mem_pestat] mean and std.dev: (214.24, 112.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 745) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1077, 3177, 5491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14319) [M::mem_pestat] mean and std.dev: (3595.55, 2834.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18733) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (813, 2638, 5391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14547) [M::mem_pestat] mean and std.dev: (3360.39, 2868.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19125) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 196.350 CPU sec, 12.700 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (900, 457422, 828, 923) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (749, 2315, 5309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14429) [M::mem_pestat] mean and std.dev: (3269.33, 2893.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18989) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 191, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591) [M::mem_pestat] mean and std.dev: (214.34, 112.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 745) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1250, 3146, 6040) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15620) [M::mem_pestat] mean and std.dev: (3808.99, 2913.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20410) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (821, 2506, 5672) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15374) [M::mem_pestat] mean and std.dev: (3437.83, 2960.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20225) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 193.631 CPU sec, 12.649 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (929, 458372, 795, 876) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (738, 2375, 5535) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15129) [M::mem_pestat] mean and std.dev: (3337.43, 2902.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19926) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 192, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 592) [M::mem_pestat] mean and std.dev: (214.75, 112.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 746) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1201, 3128, 5808) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15022) [M::mem_pestat] mean and std.dev: (3726.00, 2902.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19629) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (777, 2682, 5825) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15921) [M::mem_pestat] mean and std.dev: (3441.21, 2963.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20969) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 192.377 CPU sec, 12.558 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (927, 458737, 763, 892) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (755, 2660, 5740) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15710) [M::mem_pestat] mean and std.dev: (3463.02, 2976.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20695) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 192, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597) [M::mem_pestat] mean and std.dev: (215.63, 113.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1252, 2892, 5649) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14443) [M::mem_pestat] mean and std.dev: (3580.37, 2817.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18840) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (747, 2632, 5514) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15048) [M::mem_pestat] mean and std.dev: (3292.13, 2810.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19815) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 194.524 CPU sec, 12.441 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (978, 458147, 802, 931) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (645, 2179, 5115) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14055) [M::mem_pestat] mean and std.dev: (3184.49, 2935.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18525) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 192, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595) [M::mem_pestat] mean and std.dev: (215.60, 113.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 750) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1187, 3149, 5967) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15527) [M::mem_pestat] mean and std.dev: (3741.97, 2919.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20307) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (686, 2192, 5305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14543) [M::mem_pestat] mean and std.dev: (3164.18, 2853.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19162) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 196.838 CPU sec, 12.744 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (964, 458484, 751, 917) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (640, 2261, 5509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15247) [M::mem_pestat] mean and std.dev: (3242.31, 2894.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20116) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 193, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 598) [M::mem_pestat] mean and std.dev: (216.59, 114.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 754) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1247, 3108, 5995) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15491) [M::mem_pestat] mean and std.dev: (3818.06, 2965.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20239) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (893, 2742, 5699) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15311) [M::mem_pestat] mean and std.dev: (3462.10, 2870.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20117) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 192.503 CPU sec, 12.285 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (984, 458568, 831, 938) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (739, 2364, 5418) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14776) [M::mem_pestat] mean and std.dev: (3291.68, 2933.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19455) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 193, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 598) [M::mem_pestat] mean and std.dev: (216.14, 113.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 754) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1267, 3223, 6064) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15658) [M::mem_pestat] mean and std.dev: (3823.48, 2905.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20455) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (782, 2530, 5165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13931) [M::mem_pestat] mean and std.dev: (3254.04, 2803.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18314) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 194.386 CPU sec, 12.731 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (931, 458487, 859, 913) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (693, 2291, 5427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14895) [M::mem_pestat] mean and std.dev: (3206.76, 2873.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19629) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 192, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 600) [M::mem_pestat] mean and std.dev: (215.79, 114.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 757) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1294, 3351, 6217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16063) [M::mem_pestat] mean and std.dev: (3896.09, 2903.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20986) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (725, 2512, 5638) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15464) [M::mem_pestat] mean and std.dev: (3395.75, 2904.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20377) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 193.446 CPU sec, 12.400 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (867, 458013, 806, 896) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (633, 2462, 5494) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15216) [M::mem_pestat] mean and std.dev: (3293.57, 2898.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20077) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 191, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594) [M::mem_pestat] mean and std.dev: (214.80, 113.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 749) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1133, 3011, 5969) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15641) [M::mem_pestat] mean and std.dev: (3676.22, 2888.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20477) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (806, 2469, 5332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14384) [M::mem_pestat] mean and std.dev: (3292.34, 2843.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18910) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 192.743 CPU sec, 12.508 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (951, 457521, 769, 987) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (638, 2179, 5213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14363) [M::mem_pestat] mean and std.dev: (3177.15, 2865.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18938) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 192, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595) [M::mem_pestat] mean and std.dev: (215.48, 113.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 750) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1289, 3325, 5962) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15308) [M::mem_pestat] mean and std.dev: (3807.71, 2835.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19981) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (814, 2650, 5308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14296) [M::mem_pestat] mean and std.dev: (3308.76, 2775.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18790) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 192.628 CPU sec, 12.463 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (991, 458527, 769, 911) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (697, 2314, 5128) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13990) [M::mem_pestat] mean and std.dev: (3182.45, 2837.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18421) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 193, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595) [M::mem_pestat] mean and std.dev: (215.93, 113.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 750) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1285, 3142, 5884) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15082) [M::mem_pestat] mean and std.dev: (3679.06, 2804.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19681) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (798, 2592, 5711) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15537) [M::mem_pestat] mean and std.dev: (3377.86, 2872.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20450) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 191.885 CPU sec, 12.475 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (958, 458429, 790, 967) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (765, 2744, 5595) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15255) [M::mem_pestat] mean and std.dev: (3373.30, 2894.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20085) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 192, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595) [M::mem_pestat] mean and std.dev: (215.31, 113.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 750) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1135, 3158, 6322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16696) [M::mem_pestat] mean and std.dev: (3859.92, 2977.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21883) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (831, 2596, 6045) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16473) [M::mem_pestat] mean and std.dev: (3540.09, 3007.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21687) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 194.938 CPU sec, 12.730 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (962, 458447, 757, 914) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (738, 2373, 5557) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15195) [M::mem_pestat] mean and std.dev: (3301.10, 2881.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20014) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 192, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595) [M::mem_pestat] mean and std.dev: (215.40, 113.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 750) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1136, 3001, 5788) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15092) [M::mem_pestat] mean and std.dev: (3643.15, 2889.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19744) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (630, 2281, 5365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14835) [M::mem_pestat] mean and std.dev: (3208.74, 2905.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19570) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 194.062 CPU sec, 12.640 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (926, 458397, 814, 859) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (773, 2623, 5698) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15548) [M::mem_pestat] mean and std.dev: (3463.21, 2981.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20473) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 192, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595) [M::mem_pestat] mean and std.dev: (215.54, 113.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 750) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1182, 3159, 5658) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14610) [M::mem_pestat] mean and std.dev: (3651.38, 2792.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19086) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (688, 2170, 5439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14941) [M::mem_pestat] mean and std.dev: (3225.21, 2887.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19692) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 194.017 CPU sec, 12.493 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (925, 458184, 786, 868) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (768, 2774, 5611) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15297) [M::mem_pestat] mean and std.dev: (3404.31, 2874.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20140) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 192, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597) [M::mem_pestat] mean and std.dev: (215.41, 113.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1265, 3529, 6357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16541) [M::mem_pestat] mean and std.dev: (3963.68, 2973.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21633) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (700, 2330, 5676) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15628) [M::mem_pestat] mean and std.dev: (3300.16, 2861.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20604) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 191.866 CPU sec, 12.501 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (875, 458789, 781, 922) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (644, 2495, 5555) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15377) [M::mem_pestat] mean and std.dev: (3312.30, 2932.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20288) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 191, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591) [M::mem_pestat] mean and std.dev: (214.16, 112.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 745) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1160, 3056, 5874) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15302) [M::mem_pestat] mean and std.dev: (3714.22, 2890.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20016) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (707, 2678, 5506) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15104) [M::mem_pestat] mean and std.dev: (3343.62, 2839.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19903) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 192.473 CPU sec, 12.403 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (919, 458337, 820, 928) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (729, 2665, 5345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14577) [M::mem_pestat] mean and std.dev: (3352.31, 2902.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19193) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 190, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588) [M::mem_pestat] mean and std.dev: (212.90, 111.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 741) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1264, 3054, 5872) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15088) [M::mem_pestat] mean and std.dev: (3732.54, 2853.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19696) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (782, 2488, 5375) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14561) [M::mem_pestat] mean and std.dev: (3304.45, 2861.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19154) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 192.532 CPU sec, 12.495 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (929, 457862, 754, 903) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (614, 2173, 5194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14354) [M::mem_pestat] mean and std.dev: (3167.82, 2893.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18934) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 190, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 587) [M::mem_pestat] mean and std.dev: (212.48, 111.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 740) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1178, 3174, 6178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16178) [M::mem_pestat] mean and std.dev: (3796.63, 2884.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21178) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (784, 2697, 5281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14275) [M::mem_pestat] mean and std.dev: (3354.30, 2818.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18772) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 195.392 CPU sec, 12.593 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (925, 457846, 807, 891) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (619, 2388, 5093) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14041) [M::mem_pestat] mean and std.dev: (3160.87, 2857.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18515) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 189, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 587) [M::mem_pestat] mean and std.dev: (212.24, 111.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 740) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1211, 2916, 5912) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15314) [M::mem_pestat] mean and std.dev: (3660.77, 2921.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20015) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (688, 2661, 5322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14590) [M::mem_pestat] mean and std.dev: (3275.87, 2825.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19224) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 193.471 CPU sec, 12.502 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (896, 458279, 803, 904) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (762, 2467, 5411) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14709) [M::mem_pestat] mean and std.dev: (3382.98, 2904.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19358) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 189, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 587) [M::mem_pestat] mean and std.dev: (212.64, 111.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 740) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1143, 2878, 6129) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16101) [M::mem_pestat] mean and std.dev: (3683.54, 2881.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21087) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (548, 2240, 5067) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14105) [M::mem_pestat] mean and std.dev: (3165.51, 2943.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18624) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 191.151 CPU sec, 12.410 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (873, 457886, 784, 924) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (650, 2605, 5491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15173) [M::mem_pestat] mean and std.dev: (3349.48, 2959.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20014) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 189, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 587) [M::mem_pestat] mean and std.dev: (212.42, 111.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 740) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1211, 3056, 5772) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14894) [M::mem_pestat] mean and std.dev: (3675.08, 2844.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19455) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (516, 2656, 5496) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15456) [M::mem_pestat] mean and std.dev: (3317.73, 2934.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20436) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 194.681 CPU sec, 12.636 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (962, 457487, 803, 892) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (816, 2591, 5632) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15264) [M::mem_pestat] mean and std.dev: (3382.96, 2906.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20080) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 192, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595) [M::mem_pestat] mean and std.dev: (215.52, 113.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 750) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1120, 3229, 6219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16417) [M::mem_pestat] mean and std.dev: (3699.69, 2881.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21516) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (814, 2815, 5682) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15418) [M::mem_pestat] mean and std.dev: (3466.17, 2892.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20286) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 193.997 CPU sec, 12.574 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (892, 458038, 818, 940) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (802, 2627, 5404) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14608) [M::mem_pestat] mean and std.dev: (3397.42, 2931.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19210) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 192, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595) [M::mem_pestat] mean and std.dev: (215.56, 113.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 750) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1087, 3237, 5926) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15604) [M::mem_pestat] mean and std.dev: (3688.84, 2835.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20443) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (702, 2350, 5202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14202) [M::mem_pestat] mean and std.dev: (3185.59, 2887.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18702) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 192.833 CPU sec, 12.472 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (929, 457481, 821, 965) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (704, 2251, 5345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14627) [M::mem_pestat] mean and std.dev: (3210.70, 2849.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19268) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 192, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595) [M::mem_pestat] mean and std.dev: (215.63, 113.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 750) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1345, 3292, 6213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15949) [M::mem_pestat] mean and std.dev: (3884.31, 2971.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20817) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (819, 2518, 5506) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14880) [M::mem_pestat] mean and std.dev: (3362.49, 2921.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19567) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 193.166 CPU sec, 12.488 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (926, 458218, 804, 874) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (668, 2447, 5582) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15410) [M::mem_pestat] mean and std.dev: (3320.98, 2934.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20324) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 192, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594) [M::mem_pestat] mean and std.dev: (214.66, 113.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 749) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1149, 3226, 6042) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15828) [M::mem_pestat] mean and std.dev: (3777.86, 2882.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20721) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (848, 2368, 5436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14612) [M::mem_pestat] mean and std.dev: (3286.38, 2812.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19200) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 195.007 CPU sec, 12.648 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (942, 457862, 760, 915) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (739, 2762, 5565) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15217) [M::mem_pestat] mean and std.dev: (3390.91, 2876.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20043) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 192, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594) [M::mem_pestat] mean and std.dev: (214.96, 113.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 749) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1159, 3069, 6161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16165) [M::mem_pestat] mean and std.dev: (3787.94, 2970.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21167) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (721, 2304, 5550) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15208) [M::mem_pestat] mean and std.dev: (3320.85, 2954.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20037) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 195.592 CPU sec, 12.457 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (956, 457510, 801, 891) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (704, 2516, 5323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14561) [M::mem_pestat] mean and std.dev: (3279.16, 2848.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19180) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 192, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595) [M::mem_pestat] mean and std.dev: (215.34, 113.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 750) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1197, 3003, 5948) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15450) [M::mem_pestat] mean and std.dev: (3683.43, 2848.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20201) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (873, 2545, 5526) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14832) [M::mem_pestat] mean and std.dev: (3368.96, 2889.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19485) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 196.121 CPU sec, 12.543 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (897, 458570, 789, 957) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (617, 2300, 5443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15095) [M::mem_pestat] mean and std.dev: (3268.71, 2943.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19921) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 192, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595) [M::mem_pestat] mean and std.dev: (215.43, 113.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 750) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1096, 2643, 5647) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14749) [M::mem_pestat] mean and std.dev: (3485.42, 2845.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19300) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (866, 2813, 5345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14303) [M::mem_pestat] mean and std.dev: (3398.78, 2793.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18782) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 195.889 CPU sec, 12.691 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (955, 457142, 803, 936) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (624, 2326, 5377) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14883) [M::mem_pestat] mean and std.dev: (3211.81, 2945.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19636) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 192, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 592) [M::mem_pestat] mean and std.dev: (215.01, 113.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 746) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1114, 2969, 5614) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14614) [M::mem_pestat] mean and std.dev: (3592.98, 2849.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19114) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (734, 2698, 5959) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16409) [M::mem_pestat] mean and std.dev: (3467.57, 2988.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21634) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 194.879 CPU sec, 12.643 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (963, 458311, 862, 875) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (730, 2541, 5566) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15238) [M::mem_pestat] mean and std.dev: (3368.10, 2874.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20074) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 192, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595) [M::mem_pestat] mean and std.dev: (215.32, 113.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 750) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1000, 3180, 6101) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16303) [M::mem_pestat] mean and std.dev: (3729.18, 2916.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21404) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (845, 2718, 5710) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15440) [M::mem_pestat] mean and std.dev: (3475.82, 2936.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20305) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 191.880 CPU sec, 12.180 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (927, 458515, 826, 882) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (720, 2210, 5137) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13971) [M::mem_pestat] mean and std.dev: (3145.77, 2792.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18388) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 192, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595) [M::mem_pestat] mean and std.dev: (215.44, 113.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 750) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1046, 3029, 6236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16616) [M::mem_pestat] mean and std.dev: (3703.69, 2963.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21806) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (663, 2456, 5351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14727) [M::mem_pestat] mean and std.dev: (3225.14, 2810.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19415) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 193.817 CPU sec, 12.573 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (957, 457210, 810, 914) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (704, 2203, 5127) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13973) [M::mem_pestat] mean and std.dev: (3200.93, 2907.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18396) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 192, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595) [M::mem_pestat] mean and std.dev: (215.42, 113.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 750) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1198, 3060, 6154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16066) [M::mem_pestat] mean and std.dev: (3745.74, 2918.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21022) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (636, 2422, 5713) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15867) [M::mem_pestat] mean and std.dev: (3397.73, 3038.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20944) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 193.507 CPU sec, 12.451 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (942, 458267, 815, 906) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (684, 2384, 5290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14502) [M::mem_pestat] mean and std.dev: (3280.69, 2917.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19108) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 192, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595) [M::mem_pestat] mean and std.dev: (215.04, 113.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 750) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1378, 3484, 6423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16513) [M::mem_pestat] mean and std.dev: (3975.79, 2978.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21558) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (719, 2484, 5613) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15401) [M::mem_pestat] mean and std.dev: (3368.67, 2920.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20295) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 193.665 CPU sec, 12.545 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (910, 457521, 772, 933) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (725, 2629, 5568) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15254) [M::mem_pestat] mean and std.dev: (3418.43, 2955.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20097) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 192, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594) [M::mem_pestat] mean and std.dev: (214.70, 112.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 749) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1475, 3454, 6598) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16844) [M::mem_pestat] mean and std.dev: (4019.87, 2937.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21967) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (814, 2675, 5517) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14923) [M::mem_pestat] mean and std.dev: (3386.28, 2847.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19626) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 192.931 CPU sec, 12.516 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (921, 457990, 775, 891) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (747, 2137, 5280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14346) [M::mem_pestat] mean and std.dev: (3148.58, 2827.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18879) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 191, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591) [M::mem_pestat] mean and std.dev: (214.28, 112.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 745) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1129, 3134, 5816) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15190) [M::mem_pestat] mean and std.dev: (3686.67, 2875.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19877) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (642, 2428, 5618) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15570) [M::mem_pestat] mean and std.dev: (3319.70, 2959.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20546) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 193.399 CPU sec, 12.557 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (929, 457597, 851, 960) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (609, 2332, 5361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14865) [M::mem_pestat] mean and std.dev: (3227.38, 2911.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19617) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 192, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595) [M::mem_pestat] mean and std.dev: (215.33, 113.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 750) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1278, 3286, 5860) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15024) [M::mem_pestat] mean and std.dev: (3775.07, 2845.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19606) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (634, 2225, 5488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15196) [M::mem_pestat] mean and std.dev: (3186.68, 2881.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20050) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 193.455 CPU sec, 12.500 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (928, 458010, 773, 902) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (655, 2664, 5311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14623) [M::mem_pestat] mean and std.dev: (3317.01, 2857.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19279) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 191, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597) [M::mem_pestat] mean and std.dev: (214.96, 113.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1052, 3056, 5790) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15266) [M::mem_pestat] mean and std.dev: (3684.12, 2880.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20004) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (701, 2411, 5338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14612) [M::mem_pestat] mean and std.dev: (3271.16, 2900.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19249) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 192.804 CPU sec, 12.479 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (925, 458415, 797, 911) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (614, 2495, 5665) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15767) [M::mem_pestat] mean and std.dev: (3348.05, 2998.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20818) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 192, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 592) [M::mem_pestat] mean and std.dev: (215.05, 112.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 746) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1222, 3062, 5650) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14506) [M::mem_pestat] mean and std.dev: (3624.34, 2803.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18934) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (764, 2425, 5482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14918) [M::mem_pestat] mean and std.dev: (3305.62, 2824.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19636) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 193.478 CPU sec, 12.317 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (953, 458592, 839, 886) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (813, 2493, 5574) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15096) [M::mem_pestat] mean and std.dev: (3353.09, 2873.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19857) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 192, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 592) [M::mem_pestat] mean and std.dev: (214.90, 112.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 746) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1025, 2740, 5595) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14735) [M::mem_pestat] mean and std.dev: (3532.54, 2855.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19305) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (664, 2335, 5337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14683) [M::mem_pestat] mean and std.dev: (3195.45, 2884.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19356) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 195.302 CPU sec, 12.655 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (920, 457625, 753, 903) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (633, 2291, 5301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14637) [M::mem_pestat] mean and std.dev: (3211.81, 2916.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19305) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 191, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594) [M::mem_pestat] mean and std.dev: (214.61, 112.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 749) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1121, 2787, 5642) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14684) [M::mem_pestat] mean and std.dev: (3524.56, 2822.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19205) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (718, 2490, 5390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14734) [M::mem_pestat] mean and std.dev: (3324.56, 2856.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19406) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 194.865 CPU sec, 12.559 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (944, 457948, 801, 982) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (771, 2433, 5399) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14655) [M::mem_pestat] mean and std.dev: (3268.45, 2849.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19283) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 191, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594) [M::mem_pestat] mean and std.dev: (214.87, 113.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 749) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (886, 2936, 6273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 17047) [M::mem_pestat] mean and std.dev: (3659.23, 2985.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 22434) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (798, 2612, 5799) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15801) [M::mem_pestat] mean and std.dev: (3461.52, 2971.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20802) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 193.135 CPU sec, 12.509 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (920, 457688, 824, 811) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (747, 2513, 5706) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15624) [M::mem_pestat] mean and std.dev: (3359.55, 2918.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20583) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 191, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591) [M::mem_pestat] mean and std.dev: (213.76, 112.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 745) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1170, 3015, 5835) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15165) [M::mem_pestat] mean and std.dev: (3633.97, 2880.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19830) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (680, 2534, 5732) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15836) [M::mem_pestat] mean and std.dev: (3363.30, 2922.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20888) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 194.674 CPU sec, 12.468 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (916, 457921, 767, 948) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (732, 2555, 5334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14538) [M::mem_pestat] mean and std.dev: (3314.33, 2911.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19140) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 191, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588) [M::mem_pestat] mean and std.dev: (213.34, 111.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 741) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1042, 3128, 6027) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15997) [M::mem_pestat] mean and std.dev: (3739.96, 2977.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20982) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (721, 2413, 5494) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15040) [M::mem_pestat] mean and std.dev: (3317.62, 2858.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19813) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 196.083 CPU sec, 12.660 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (861, 457774, 757, 888) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (732, 2237, 5206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14154) [M::mem_pestat] mean and std.dev: (3180.59, 2850.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18628) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 190, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 585) [M::mem_pestat] mean and std.dev: (212.70, 111.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 737) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1297, 3257, 5972) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15322) [M::mem_pestat] mean and std.dev: (3840.08, 2904.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19997) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (725, 2600, 5381) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14693) [M::mem_pestat] mean and std.dev: (3338.70, 2882.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19349) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 193.243 CPU sec, 12.798 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (875, 457818, 839, 850) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (616, 2509, 5337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14779) [M::mem_pestat] mean and std.dev: (3277.85, 2941.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19500) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 190, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 587) [M::mem_pestat] mean and std.dev: (212.40, 111.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 740) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1071, 3020, 6163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16347) [M::mem_pestat] mean and std.dev: (3697.70, 2972.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21439) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (720, 2340, 5180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14100) [M::mem_pestat] mean and std.dev: (3191.42, 2798.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18560) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 193.954 CPU sec, 12.708 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (885, 457917, 837, 885) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (761, 2470, 5389) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14645) [M::mem_pestat] mean and std.dev: (3297.47, 2875.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19273) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 189, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 587) [M::mem_pestat] mean and std.dev: (212.38, 111.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 740) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1138, 3006, 6125) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16099) [M::mem_pestat] mean and std.dev: (3728.95, 2952.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21086) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (816, 2743, 5891) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16041) [M::mem_pestat] mean and std.dev: (3512.01, 2982.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21116) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 195.573 CPU sec, 12.524 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (852, 457286, 737, 845) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (656, 2317, 5543) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15317) [M::mem_pestat] mean and std.dev: (3219.94, 2887.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20204) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 190, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 587) [M::mem_pestat] mean and std.dev: (212.51, 111.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 740) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1484, 3151, 6059) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15209) [M::mem_pestat] mean and std.dev: (3811.40, 2826.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19784) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (759, 2574, 5675) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15507) [M::mem_pestat] mean and std.dev: (3403.52, 2943.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20423) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 195.598 CPU sec, 12.670 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (873, 458014, 787, 879) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (779, 2498, 5464) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14834) [M::mem_pestat] mean and std.dev: (3316.72, 2898.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19519) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 190, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 585) [M::mem_pestat] mean and std.dev: (212.44, 111.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 737) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1186, 3091, 5817) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15079) [M::mem_pestat] mean and std.dev: (3721.85, 2877.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19710) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (803, 2504, 5643) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15323) [M::mem_pestat] mean and std.dev: (3332.67, 2896.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20163) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 197.044 CPU sec, 12.593 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (880, 458133, 753, 911) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (725, 2355, 5402) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14756) [M::mem_pestat] mean and std.dev: (3274.91, 2906.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19433) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 191, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588) [M::mem_pestat] mean and std.dev: (213.30, 112.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 741) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1156, 3234, 6420) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16948) [M::mem_pestat] mean and std.dev: (3828.76, 2929.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 22212) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (724, 2400, 5403) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14761) [M::mem_pestat] mean and std.dev: (3284.54, 2882.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19440) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 194.447 CPU sec, 12.598 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (881, 457647, 804, 944) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (580, 1979, 5318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14794) [M::mem_pestat] mean and std.dev: (3166.81, 2929.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19532) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 193, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 598) [M::mem_pestat] mean and std.dev: (216.37, 114.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 754) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1325, 3438, 6112) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15686) [M::mem_pestat] mean and std.dev: (3944.57, 2934.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20473) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (849, 2777, 6069) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16509) [M::mem_pestat] mean and std.dev: (3579.75, 2993.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21729) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 194.549 CPU sec, 12.532 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (937, 457896, 855, 851) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (745, 2504, 5564) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15202) [M::mem_pestat] mean and std.dev: (3331.09, 2843.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20021) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 193, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 598) [M::mem_pestat] mean and std.dev: (216.48, 113.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 754) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1203, 3094, 5758) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14868) [M::mem_pestat] mean and std.dev: (3719.13, 2828.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19423) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (672, 2507, 5336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14664) [M::mem_pestat] mean and std.dev: (3294.15, 2894.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19328) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 191.849 CPU sec, 12.486 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (921, 457934, 852, 867) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (764, 2502, 5761) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15755) [M::mem_pestat] mean and std.dev: (3441.60, 2967.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20752) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 192, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 598) [M::mem_pestat] mean and std.dev: (215.97, 114.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 754) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1374, 3139, 6192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15828) [M::mem_pestat] mean and std.dev: (3858.39, 2885.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20646) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (787, 2289, 5280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14266) [M::mem_pestat] mean and std.dev: (3237.60, 2849.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18759) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 192.331 CPU sec, 12.488 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (929, 457898, 749, 910) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (686, 2538, 5401) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14831) [M::mem_pestat] mean and std.dev: (3287.84, 2849.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19546) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 192, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595) [M::mem_pestat] mean and std.dev: (215.45, 113.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 750) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1146, 3137, 5686) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14766) [M::mem_pestat] mean and std.dev: (3612.92, 2834.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19306) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (720, 2299, 4906) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13278) [M::mem_pestat] mean and std.dev: (3121.87, 2740.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17464) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 191.714 CPU sec, 12.464 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (912, 457826, 808, 953) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (610, 2387, 5527) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15361) [M::mem_pestat] mean and std.dev: (3291.07, 2958.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20278) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 192, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595) [M::mem_pestat] mean and std.dev: (215.34, 113.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 750) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (896, 2758, 5550) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14858) [M::mem_pestat] mean and std.dev: (3490.82, 2853.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19512) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (861, 2586, 6042) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16404) [M::mem_pestat] mean and std.dev: (3492.24, 2959.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21585) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 193.053 CPU sec, 12.598 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (933, 457708, 882, 927) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (669, 2262, 5405) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14877) [M::mem_pestat] mean and std.dev: (3295.62, 2955.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19613) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 192, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595) [M::mem_pestat] mean and std.dev: (215.76, 113.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 750) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1326, 3311, 6061) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15531) [M::mem_pestat] mean and std.dev: (3799.38, 2856.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20266) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (684, 2523, 5603) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15441) [M::mem_pestat] mean and std.dev: (3352.30, 2937.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20360) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 192.535 CPU sec, 12.455 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (890, 457791, 818, 897) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (583, 2221, 5033) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13933) [M::mem_pestat] mean and std.dev: (3084.16, 2832.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18383) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 192, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595) [M::mem_pestat] mean and std.dev: (215.33, 113.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 750) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1095, 3038, 6016) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15858) [M::mem_pestat] mean and std.dev: (3679.95, 2885.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20779) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (676, 2203, 5210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14278) [M::mem_pestat] mean and std.dev: (3181.70, 2868.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18812) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 193.624 CPU sec, 12.341 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (890, 458474, 809, 885) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (697, 2454, 5380) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14746) [M::mem_pestat] mean and std.dev: (3248.20, 2855.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19429) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 192, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 592) [M::mem_pestat] mean and std.dev: (215.03, 113.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 746) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1140, 3311, 6150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16170) [M::mem_pestat] mean and std.dev: (3825.11, 2939.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21180) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1006, 2531, 5338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14002) [M::mem_pestat] mean and std.dev: (3376.17, 2805.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18334) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 196.966 CPU sec, 12.817 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (921, 458166, 807, 893) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (572, 2585, 5307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14777) [M::mem_pestat] mean and std.dev: (3272.26, 2851.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19512) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 192, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595) [M::mem_pestat] mean and std.dev: (215.66, 113.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 750) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1265, 2975, 5966) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15368) [M::mem_pestat] mean and std.dev: (3756.09, 2865.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20069) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (787, 2537, 5750) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15676) [M::mem_pestat] mean and std.dev: (3399.04, 2932.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20639) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 193.914 CPU sec, 12.547 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (893, 457689, 771, 935) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (723, 2460, 5200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14154) [M::mem_pestat] mean and std.dev: (3227.11, 2791.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18631) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 192, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595) [M::mem_pestat] mean and std.dev: (215.48, 113.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 750) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1320, 3160, 5923) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15129) [M::mem_pestat] mean and std.dev: (3765.51, 2826.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19732) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (674, 2329, 5260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14432) [M::mem_pestat] mean and std.dev: (3195.63, 2826.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19018) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 196.447 CPU sec, 12.846 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (883, 457942, 862, 944) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (713, 2716, 5553) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15233) [M::mem_pestat] mean and std.dev: (3432.88, 2927.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20073) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 192, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 592) [M::mem_pestat] mean and std.dev: (214.97, 112.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 746) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1350, 3186, 6044) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15432) [M::mem_pestat] mean and std.dev: (3830.91, 2895.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20126) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (762, 2482, 5226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14154) [M::mem_pestat] mean and std.dev: (3292.20, 2853.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18618) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 195.391 CPU sec, 12.620 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (882, 458575, 796, 889) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (697, 2286, 5567) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15307) [M::mem_pestat] mean and std.dev: (3235.99, 2866.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20177) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 192, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 592) [M::mem_pestat] mean and std.dev: (215.08, 112.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 746) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1166, 3055, 6053) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15827) [M::mem_pestat] mean and std.dev: (3672.80, 2859.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20714) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (690, 2287, 5481) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15063) [M::mem_pestat] mean and std.dev: (3286.30, 2939.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19854) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 194.198 CPU sec, 12.673 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (910, 457636, 736, 949) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (662, 2326, 5268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14480) [M::mem_pestat] mean and std.dev: (3194.01, 2865.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19086) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 191, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591) [M::mem_pestat] mean and std.dev: (214.21, 112.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 745) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1105, 3108, 6104) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16102) [M::mem_pestat] mean and std.dev: (3732.33, 2906.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21101) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (700, 2548, 5722) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15766) [M::mem_pestat] mean and std.dev: (3426.94, 2984.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20788) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 194.804 CPU sec, 12.568 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (947, 458753, 788, 929) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (702, 2541, 5528) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15180) [M::mem_pestat] mean and std.dev: (3351.03, 2935.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20006) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 192, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 589) [M::mem_pestat] mean and std.dev: (214.37, 112.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 742) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1124, 2919, 5708) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14876) [M::mem_pestat] mean and std.dev: (3621.94, 2834.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19460) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (731, 2263, 5138) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13952) [M::mem_pestat] mean and std.dev: (3173.49, 2843.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18359) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 196.035 CPU sec, 12.702 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (924, 458245, 814, 884) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (672, 2434, 5565) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15351) [M::mem_pestat] mean and std.dev: (3298.65, 2907.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20244) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 191, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591) [M::mem_pestat] mean and std.dev: (214.29, 112.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 745) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1327, 3332, 6256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16114) [M::mem_pestat] mean and std.dev: (3862.03, 2883.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21043) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (874, 2899, 5738) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15466) [M::mem_pestat] mean and std.dev: (3521.70, 2894.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20330) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 195.868 CPU sec, 12.472 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (902, 458050, 775, 835) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (780, 2461, 5585) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15195) [M::mem_pestat] mean and std.dev: (3326.94, 2933.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20000) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 191, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 589) [M::mem_pestat] mean and std.dev: (214.45, 112.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 742) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1136, 3301, 6134) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16130) [M::mem_pestat] mean and std.dev: (3832.17, 2890.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21128) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (713, 2404, 5227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14255) [M::mem_pestat] mean and std.dev: (3209.11, 2839.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18769) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 198.883 CPU sec, 12.815 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (906, 458159, 758, 892) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (702, 2560, 5469) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15003) [M::mem_pestat] mean and std.dev: (3370.08, 2961.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19770) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 191, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 589) [M::mem_pestat] mean and std.dev: (214.30, 112.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 742) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1221, 3135, 5931) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15351) [M::mem_pestat] mean and std.dev: (3754.84, 2932.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20061) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (856, 2549, 5280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14128) [M::mem_pestat] mean and std.dev: (3311.78, 2790.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18552) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 194.397 CPU sec, 12.415 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (902, 458695, 887, 872) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (638, 2465, 5665) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15719) [M::mem_pestat] mean and std.dev: (3313.84, 2930.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20746) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 191, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 589) [M::mem_pestat] mean and std.dev: (213.99, 111.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 742) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1295, 3338, 6244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16142) [M::mem_pestat] mean and std.dev: (3934.95, 2928.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21091) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (761, 2177, 5421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14741) [M::mem_pestat] mean and std.dev: (3253.43, 2868.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19401) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 191.048 CPU sec, 12.376 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (919, 457126, 778, 908) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (621, 2043, 5539) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15375) [M::mem_pestat] mean and std.dev: (3208.35, 2946.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20293) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 190, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591) [M::mem_pestat] mean and std.dev: (213.65, 112.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 745) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1242, 3203, 6229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16203) [M::mem_pestat] mean and std.dev: (3879.16, 2890.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21190) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (702, 2362, 5380) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14736) [M::mem_pestat] mean and std.dev: (3259.98, 2889.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19414) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 192.641 CPU sec, 12.553 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (867, 457961, 816, 951) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (729, 2432, 5653) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15501) [M::mem_pestat] mean and std.dev: (3341.34, 2947.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20425) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 191, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588) [M::mem_pestat] mean and std.dev: (213.39, 111.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 741) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1214, 3276, 5625) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14447) [M::mem_pestat] mean and std.dev: (3718.18, 2789.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18858) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (702, 2316, 5126) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13974) [M::mem_pestat] mean and std.dev: (3219.03, 2869.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18398) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 192.939 CPU sec, 12.514 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (826, 458212, 789, 889) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (804, 2603, 5502) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14898) [M::mem_pestat] mean and std.dev: (3408.47, 2906.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19596) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 189, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 582) [M::mem_pestat] mean and std.dev: (211.91, 110.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 733) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1001, 2929, 5957) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15869) [M::mem_pestat] mean and std.dev: (3583.70, 2860.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20825) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (731, 1941, 5365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14633) [M::mem_pestat] mean and std.dev: (3179.36, 2917.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19267) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 196.232 CPU sec, 12.667 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (916, 457929, 790, 868) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (676, 2221, 5171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14161) [M::mem_pestat] mean and std.dev: (3137.06, 2804.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18656) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 189, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 584) [M::mem_pestat] mean and std.dev: (211.92, 110.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 736) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1284, 3429, 6450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16782) [M::mem_pestat] mean and std.dev: (3932.79, 2980.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21948) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (767, 2411, 5373) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14585) [M::mem_pestat] mean and std.dev: (3274.47, 2807.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19191) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 195.606 CPU sec, 12.730 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (867, 457750, 785, 893) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (648, 2263, 5237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14415) [M::mem_pestat] mean and std.dev: (3169.79, 2828.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19004) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 189, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 581) [M::mem_pestat] mean and std.dev: (211.24, 110.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 732) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1130, 2889, 5871) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15353) [M::mem_pestat] mean and std.dev: (3633.40, 2901.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20094) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (837, 2686, 5612) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15162) [M::mem_pestat] mean and std.dev: (3396.72, 2802.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19937) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 194.103 CPU sec, 12.393 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (849, 457571, 763, 892) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (661, 2458, 5432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14974) [M::mem_pestat] mean and std.dev: (3330.09, 2938.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19745) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 189, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 584) [M::mem_pestat] mean and std.dev: (211.76, 110.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 736) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1137, 3123, 5900) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15426) [M::mem_pestat] mean and std.dev: (3700.85, 2808.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20189) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (779, 2682, 5922) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16208) [M::mem_pestat] mean and std.dev: (3483.67, 2991.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21351) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 197.504 CPU sec, 12.794 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (929, 458198, 745, 900) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (826, 2702, 5555) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15013) [M::mem_pestat] mean and std.dev: (3400.53, 2839.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19742) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 189, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 584) [M::mem_pestat] mean and std.dev: (211.59, 110.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 736) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1005, 2879, 5670) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15000) [M::mem_pestat] mean and std.dev: (3556.38, 2884.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19665) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (806, 2510, 5824) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15860) [M::mem_pestat] mean and std.dev: (3394.89, 2922.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20878) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 195.531 CPU sec, 12.780 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (869, 457541, 803, 865) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (695, 2189, 5633) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15509) [M::mem_pestat] mean and std.dev: (3290.76, 3020.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20447) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 189, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 584) [M::mem_pestat] mean and std.dev: (211.73, 110.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 736) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1232, 3155, 5736) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14744) [M::mem_pestat] mean and std.dev: (3692.72, 2792.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19248) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (689, 2572, 5293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14501) [M::mem_pestat] mean and std.dev: (3295.44, 2865.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19105) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 196.974 CPU sec, 12.783 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (900, 457181, 800, 852) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (636, 2386, 5339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14745) [M::mem_pestat] mean and std.dev: (3199.41, 2875.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19448) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 189, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 584) [M::mem_pestat] mean and std.dev: (211.75, 110.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 736) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1184, 3310, 6135) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16037) [M::mem_pestat] mean and std.dev: (3813.73, 2897.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20988) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (721, 2432, 5229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14245) [M::mem_pestat] mean and std.dev: (3209.24, 2810.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18753) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 195.624 CPU sec, 12.595 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (909, 457443, 773, 927) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (617, 2291, 5105) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14081) [M::mem_pestat] mean and std.dev: (3158.79, 2875.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18569) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 192, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595) [M::mem_pestat] mean and std.dev: (215.74, 113.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 750) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1118, 3202, 6195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16349) [M::mem_pestat] mean and std.dev: (3831.14, 2974.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21426) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (667, 2175, 4999) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13663) [M::mem_pestat] mean and std.dev: (3058.61, 2782.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17995) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 195.576 CPU sec, 12.860 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (897, 458086, 786, 878) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (621, 2300, 4904) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13470) [M::mem_pestat] mean and std.dev: (3142.52, 2859.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17753) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 193, 287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 601) [M::mem_pestat] mean and std.dev: (216.71, 114.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 758) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1133, 3137, 6079) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15971) [M::mem_pestat] mean and std.dev: (3726.96, 2884.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20917) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (717, 2432, 5470) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14976) [M::mem_pestat] mean and std.dev: (3247.84, 2839.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19729) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 197.440 CPU sec, 12.803 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (944, 457854, 831, 964) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (657, 2451, 5729) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15873) [M::mem_pestat] mean and std.dev: (3356.10, 2952.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20945) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 193, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 598) [M::mem_pestat] mean and std.dev: (216.33, 113.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 754) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1165, 3072, 5912) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15406) [M::mem_pestat] mean and std.dev: (3692.34, 2888.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20153) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (724, 2583, 5681) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15595) [M::mem_pestat] mean and std.dev: (3382.02, 2931.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20552) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 194.876 CPU sec, 12.637 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (943, 458283, 787, 905) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (685, 2325, 5362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14716) [M::mem_pestat] mean and std.dev: (3281.36, 2893.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19393) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 193, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 598) [M::mem_pestat] mean and std.dev: (216.21, 113.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 754) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1032, 3176, 6104) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16248) [M::mem_pestat] mean and std.dev: (3703.82, 2910.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21320) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (777, 2579, 5944) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16278) [M::mem_pestat] mean and std.dev: (3498.63, 3004.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21445) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 194.829 CPU sec, 12.563 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (971, 457690, 823, 921) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (740, 2304, 5211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14153) [M::mem_pestat] mean and std.dev: (3258.81, 2902.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18624) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 193, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595) [M::mem_pestat] mean and std.dev: (215.95, 113.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 750) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1451, 3408, 6200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15698) [M::mem_pestat] mean and std.dev: (3952.62, 2879.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20447) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (722, 2308, 5407) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14777) [M::mem_pestat] mean and std.dev: (3200.63, 2827.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19462) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 196.163 CPU sec, 12.683 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (889, 457404, 775, 915) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (705, 2644, 5351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14643) [M::mem_pestat] mean and std.dev: (3331.98, 2827.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19289) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 192, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595) [M::mem_pestat] mean and std.dev: (215.41, 113.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 750) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1232, 3281, 6197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16127) [M::mem_pestat] mean and std.dev: (3875.44, 2949.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21092) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (627, 2441, 5591) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15519) [M::mem_pestat] mean and std.dev: (3330.08, 3011.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20483) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 196.266 CPU sec, 12.472 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (940, 457683, 859, 909) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (766, 2455, 5281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14311) [M::mem_pestat] mean and std.dev: (3271.51, 2859.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18826) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 192, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 592) [M::mem_pestat] mean and std.dev: (214.78, 112.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 746) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1086, 3165, 6028) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15912) [M::mem_pestat] mean and std.dev: (3708.98, 2925.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20854) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (758, 2338, 5647) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15425) [M::mem_pestat] mean and std.dev: (3315.16, 2916.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20314) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 193.851 CPU sec, 12.597 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (913, 457889, 800, 877) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (744, 2747, 5867) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16113) [M::mem_pestat] mean and std.dev: (3448.14, 2969.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21236) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 191, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 592) [M::mem_pestat] mean and std.dev: (214.50, 112.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 746) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1191, 2969, 5809) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15045) [M::mem_pestat] mean and std.dev: (3654.64, 2841.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19663) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (923, 2603, 5364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14246) [M::mem_pestat] mean and std.dev: (3413.40, 2874.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18687) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 195.769 CPU sec, 12.750 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (952, 458230, 816, 952) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (681, 2469, 5576) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15366) [M::mem_pestat] mean and std.dev: (3278.27, 2872.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20261) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 192, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594) [M::mem_pestat] mean and std.dev: (214.59, 112.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 749) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1374, 3323, 6463) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16641) [M::mem_pestat] mean and std.dev: (3960.73, 3013.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21730) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (787, 2614, 5376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14554) [M::mem_pestat] mean and std.dev: (3333.59, 2821.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19143) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 195.602 CPU sec, 12.759 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (968, 458276, 763, 912) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (587, 2267, 5229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14513) [M::mem_pestat] mean and std.dev: (3107.51, 2832.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19155) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 192, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595) [M::mem_pestat] mean and std.dev: (215.16, 113.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 750) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1254, 3125, 6432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16788) [M::mem_pestat] mean and std.dev: (3932.70, 3033.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21966) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (746, 2494, 5497) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14999) [M::mem_pestat] mean and std.dev: (3316.43, 2863.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19750) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 197.675 CPU sec, 12.733 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (948, 457812, 783, 980) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (721, 2403, 5215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14203) [M::mem_pestat] mean and std.dev: (3214.77, 2851.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18697) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 192, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595) [M::mem_pestat] mean and std.dev: (215.33, 113.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 750) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (900, 2851, 5791) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15573) [M::mem_pestat] mean and std.dev: (3509.31, 2866.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20464) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (685, 2237, 5567) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15331) [M::mem_pestat] mean and std.dev: (3242.25, 2898.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20213) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 202.094 CPU sec, 13.119 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (847, 459124, 766, 914) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (656, 2590, 5235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14393) [M::mem_pestat] mean and std.dev: (3314.07, 2902.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18972) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 192, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595) [M::mem_pestat] mean and std.dev: (215.35, 113.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 750) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1267, 3355, 6530) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 17056) [M::mem_pestat] mean and std.dev: (3965.19, 3014.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 22319) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (774, 2395, 5170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13962) [M::mem_pestat] mean and std.dev: (3233.45, 2820.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18358) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 202.828 CPU sec, 12.915 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (992, 458461, 867, 887) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (702, 2326, 5488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15060) [M::mem_pestat] mean and std.dev: (3288.77, 2943.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19846) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 191, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591) [M::mem_pestat] mean and std.dev: (214.34, 112.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 745) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1176, 3167, 6315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16593) [M::mem_pestat] mean and std.dev: (3799.07, 2955.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21732) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (707, 2195, 4819) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13043) [M::mem_pestat] mean and std.dev: (3062.95, 2765.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17155) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 204.354 CPU sec, 13.182 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (906, 458178, 743, 918) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (622, 2222, 5151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14209) [M::mem_pestat] mean and std.dev: (3097.93, 2783.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18738) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 191, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 585) [M::mem_pestat] mean and std.dev: (212.96, 111.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 737) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1289, 3212, 6386) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16580) [M::mem_pestat] mean and std.dev: (3940.35, 3012.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21677) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (695, 2318, 5568) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15314) [M::mem_pestat] mean and std.dev: (3240.76, 2934.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20187) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 203.278 CPU sec, 12.922 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (945, 458636, 740, 891) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (654, 2486, 5710) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15822) [M::mem_pestat] mean and std.dev: (3365.65, 2984.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20878) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 192, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591) [M::mem_pestat] mean and std.dev: (214.45, 112.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 745) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1202, 3041, 6332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16592) [M::mem_pestat] mean and std.dev: (3810.96, 2943.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21722) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (744, 2589, 5375) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14637) [M::mem_pestat] mean and std.dev: (3312.92, 2849.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19268) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 204.044 CPU sec, 13.182 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (939, 458768, 785, 899) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (679, 2504, 5269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14449) [M::mem_pestat] mean and std.dev: (3268.93, 2860.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19039) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 191, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594) [M::mem_pestat] mean and std.dev: (214.58, 112.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 749) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1032, 2738, 5749) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15183) [M::mem_pestat] mean and std.dev: (3523.26, 2899.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19900) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (840, 2784, 5828) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15804) [M::mem_pestat] mean and std.dev: (3507.36, 2884.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20792) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 203.254 CPU sec, 13.173 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (970, 458870, 774, 899) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (663, 2273, 5479) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15111) [M::mem_pestat] mean and std.dev: (3264.54, 2906.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19927) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 191, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591) [M::mem_pestat] mean and std.dev: (214.02, 112.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 745) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1211, 3073, 5779) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14915) [M::mem_pestat] mean and std.dev: (3648.92, 2912.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19483) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (768, 2618, 5358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14538) [M::mem_pestat] mean and std.dev: (3280.98, 2758.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19128) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 202.418 CPU sec, 13.067 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (956, 458084, 803, 898) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (690, 2648, 5654) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15582) [M::mem_pestat] mean and std.dev: (3416.07, 2952.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20546) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 191, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591) [M::mem_pestat] mean and std.dev: (214.23, 112.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 745) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1251, 3283, 5922) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15264) [M::mem_pestat] mean and std.dev: (3758.26, 2872.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19935) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (709, 2485, 5630) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15472) [M::mem_pestat] mean and std.dev: (3358.51, 2892.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20393) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 203.195 CPU sec, 12.910 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (907, 458300, 775, 866) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (689, 2509, 5398) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14816) [M::mem_pestat] mean and std.dev: (3337.46, 2933.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19525) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 190, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 585) [M::mem_pestat] mean and std.dev: (212.90, 111.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 737) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1193, 3251, 5908) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15338) [M::mem_pestat] mean and std.dev: (3780.20, 2898.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20053) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (691, 2336, 5785) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15973) [M::mem_pestat] mean and std.dev: (3324.26, 2976.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21067) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 203.401 CPU sec, 13.301 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (889, 458231, 798, 912) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (800, 2515, 5501) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14903) [M::mem_pestat] mean and std.dev: (3321.25, 2849.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19604) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 190, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 585) [M::mem_pestat] mean and std.dev: (212.76, 111.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 737) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1143, 2928, 5869) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15321) [M::mem_pestat] mean and std.dev: (3650.47, 2921.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20047) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (714, 2364, 5509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15099) [M::mem_pestat] mean and std.dev: (3329.13, 2875.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19894) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 203.024 CPU sec, 12.950 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (888, 458500, 749, 827) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (676, 2370, 5358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14722) [M::mem_pestat] mean and std.dev: (3267.90, 2876.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19404) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 190, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 587) [M::mem_pestat] mean and std.dev: (212.57, 111.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 740) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1168, 3350, 5918) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15418) [M::mem_pestat] mean and std.dev: (3766.06, 2838.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20168) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (550, 2043, 5320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14860) [M::mem_pestat] mean and std.dev: (3190.72, 2921.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19630) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 204.670 CPU sec, 13.258 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (856, 458921, 852, 804) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (732, 2296, 4775) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12861) [M::mem_pestat] mean and std.dev: (3063.82, 2705.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16904) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 189, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 585) [M::mem_pestat] mean and std.dev: (212.04, 110.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 737) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1189, 3054, 6110) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15952) [M::mem_pestat] mean and std.dev: (3757.62, 2928.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20873) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (858, 2480, 5506) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14802) [M::mem_pestat] mean and std.dev: (3443.40, 2946.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19450) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 202.414 CPU sec, 12.879 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (899, 458606, 775, 871) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (850, 2404, 5411) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14533) [M::mem_pestat] mean and std.dev: (3305.64, 2859.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19094) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 189, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 581) [M::mem_pestat] mean and std.dev: (211.22, 110.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 732) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1119, 2706, 5407) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13983) [M::mem_pestat] mean and std.dev: (3451.07, 2827.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18271) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (681, 2268, 5365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14733) [M::mem_pestat] mean and std.dev: (3243.28, 2908.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19417) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 204.667 CPU sec, 13.153 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (942, 458270, 722, 908) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (625, 2328, 5595) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15535) [M::mem_pestat] mean and std.dev: (3282.67, 2934.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20505) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 189, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 581) [M::mem_pestat] mean and std.dev: (211.01, 110.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 732) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1233, 3066, 6136) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15942) [M::mem_pestat] mean and std.dev: (3746.29, 2960.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20845) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (645, 2151, 5206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14328) [M::mem_pestat] mean and std.dev: (3130.22, 2870.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18889) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 202.094 CPU sec, 12.864 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (915, 457589, 781, 936) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (688, 2262, 5583) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15373) [M::mem_pestat] mean and std.dev: (3292.36, 2966.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20268) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 189, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 581) [M::mem_pestat] mean and std.dev: (211.46, 110.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 732) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1342, 3269, 6396) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16504) [M::mem_pestat] mean and std.dev: (3891.86, 2932.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21558) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (814, 2546, 5512) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14908) [M::mem_pestat] mean and std.dev: (3350.12, 2922.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19606) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 205.985 CPU sec, 13.306 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (925, 458233, 757, 908) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (847, 2731, 5652) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15262) [M::mem_pestat] mean and std.dev: (3432.89, 2870.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20067) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 189, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 584) [M::mem_pestat] mean and std.dev: (211.71, 110.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 736) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1022, 2832, 5725) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15131) [M::mem_pestat] mean and std.dev: (3568.55, 2855.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19834) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (851, 2644, 5798) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15692) [M::mem_pestat] mean and std.dev: (3507.62, 3006.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20639) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 202.163 CPU sec, 12.889 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (895, 458008, 793, 897) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (618, 2470, 5400) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14964) [M::mem_pestat] mean and std.dev: (3280.60, 2953.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19746) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 189, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 584) [M::mem_pestat] mean and std.dev: (211.93, 110.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 736) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1236, 3243, 6329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16515) [M::mem_pestat] mean and std.dev: (3871.07, 2988.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21608) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (681, 2242, 5379) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14775) [M::mem_pestat] mean and std.dev: (3267.43, 2950.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19473) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 202.104 CPU sec, 13.015 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (910, 457293, 792, 910) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (736, 2420, 5953) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16387) [M::mem_pestat] mean and std.dev: (3441.26, 3003.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21604) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 190, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 585) [M::mem_pestat] mean and std.dev: (212.84, 111.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 737) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1244, 3185, 6393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16691) [M::mem_pestat] mean and std.dev: (3909.15, 2954.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21840) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (728, 2725, 5912) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16280) [M::mem_pestat] mean and std.dev: (3487.06, 2998.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21464) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 202.712 CPU sec, 12.893 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (942, 457518, 868, 883) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (676, 2452, 5483) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15097) [M::mem_pestat] mean and std.dev: (3249.06, 2842.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19904) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 193, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 598) [M::mem_pestat] mean and std.dev: (216.16, 114.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 754) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1215, 3193, 5855) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15135) [M::mem_pestat] mean and std.dev: (3764.96, 2847.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19775) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (804, 2613, 5955) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16257) [M::mem_pestat] mean and std.dev: (3480.19, 2967.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21408) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 203.774 CPU sec, 13.141 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (861, 458387, 825, 908) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (614, 2371, 5265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14567) [M::mem_pestat] mean and std.dev: (3234.59, 2923.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19218) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 193, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 598) [M::mem_pestat] mean and std.dev: (216.35, 114.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 754) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1316, 3237, 5788) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14732) [M::mem_pestat] mean and std.dev: (3738.89, 2814.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19204) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (758, 2420, 5704) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15596) [M::mem_pestat] mean and std.dev: (3400.20, 2998.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20542) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 204.711 CPU sec, 13.182 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (919, 458144, 770, 920) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (670, 2436, 5643) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15589) [M::mem_pestat] mean and std.dev: (3385.47, 3011.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20562) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 193, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 598) [M::mem_pestat] mean and std.dev: (216.42, 113.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 754) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1082, 2864, 5421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14099) [M::mem_pestat] mean and std.dev: (3523.78, 2798.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18438) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (676, 2226, 5287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14509) [M::mem_pestat] mean and std.dev: (3160.47, 2849.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19120) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 203.284 CPU sec, 13.189 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (916, 458250, 759, 869) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (665, 2240, 5215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14315) [M::mem_pestat] mean and std.dev: (3250.70, 2946.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18865) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 193, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 598) [M::mem_pestat] mean and std.dev: (216.09, 113.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 754) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1155, 2955, 5492) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14166) [M::mem_pestat] mean and std.dev: (3525.75, 2692.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18503) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (755, 2474, 5510) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15020) [M::mem_pestat] mean and std.dev: (3331.89, 2894.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19775) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 202.874 CPU sec, 12.900 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (876, 457998, 805, 896) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (786, 2523, 5241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14151) [M::mem_pestat] mean and std.dev: (3254.64, 2817.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18606) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 192, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595) [M::mem_pestat] mean and std.dev: (215.50, 113.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 750) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1265, 2951, 6025) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15545) [M::mem_pestat] mean and std.dev: (3682.39, 2820.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20305) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (635, 2259, 5397) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14921) [M::mem_pestat] mean and std.dev: (3149.91, 2852.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19683) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 203.991 CPU sec, 13.166 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (924, 458416, 785, 905) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (679, 2523, 5418) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14896) [M::mem_pestat] mean and std.dev: (3325.31, 2948.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19635) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 192, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595) [M::mem_pestat] mean and std.dev: (215.36, 113.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 750) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1292, 3026, 6020) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15476) [M::mem_pestat] mean and std.dev: (3671.78, 2864.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20204) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (638, 2220, 5169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14231) [M::mem_pestat] mean and std.dev: (3195.98, 2937.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18762) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 202.912 CPU sec, 12.890 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (921, 458868, 780, 807) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (666, 2397, 5522) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15234) [M::mem_pestat] mean and std.dev: (3301.73, 2936.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20090) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 192, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595) [M::mem_pestat] mean and std.dev: (215.22, 113.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 750) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1225, 3525, 6255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16315) [M::mem_pestat] mean and std.dev: (3918.23, 2907.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21345) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (679, 2265, 4902) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13348) [M::mem_pestat] mean and std.dev: (3113.99, 2804.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17571) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 204.743 CPU sec, 13.235 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (843, 457799, 807, 952) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (764, 2495, 5330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14462) [M::mem_pestat] mean and std.dev: (3299.46, 2852.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19028) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 191, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591) [M::mem_pestat] mean and std.dev: (214.30, 112.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 745) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (997, 3053, 6090) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16276) [M::mem_pestat] mean and std.dev: (3720.65, 2976.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21369) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (776, 2488, 5624) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15320) [M::mem_pestat] mean and std.dev: (3363.62, 2963.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20168) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 204.309 CPU sec, 13.024 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (921, 457799, 763, 892) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (698, 2417, 5348) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14648) [M::mem_pestat] mean and std.dev: (3250.32, 2870.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19298) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 192, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595) [M::mem_pestat] mean and std.dev: (215.36, 113.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 750) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1207, 3009, 6200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16186) [M::mem_pestat] mean and std.dev: (3714.84, 2923.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21179) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (656, 2086, 5015) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13733) [M::mem_pestat] mean and std.dev: (3072.81, 2831.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18092) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 203.450 CPU sec, 13.158 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (981, 458246, 823, 969) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (777, 2353, 5474) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14868) [M::mem_pestat] mean and std.dev: (3288.53, 2870.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19565) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 192, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595) [M::mem_pestat] mean and std.dev: (215.52, 113.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 750) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1147, 3416, 6359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16783) [M::mem_pestat] mean and std.dev: (3899.78, 2923.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21995) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (894, 2862, 6042) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16338) [M::mem_pestat] mean and std.dev: (3576.59, 2960.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21486) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 204.742 CPU sec, 13.039 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (942, 458832, 792, 951) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (577, 2120, 5556) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15514) [M::mem_pestat] mean and std.dev: (3208.72, 2971.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20493) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 193, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 598) [M::mem_pestat] mean and std.dev: (216.12, 113.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 754) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1099, 3079, 6030) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15892) [M::mem_pestat] mean and std.dev: (3652.13, 2891.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20823) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (840, 2851, 5757) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15591) [M::mem_pestat] mean and std.dev: (3496.38, 2953.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20508) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 203.826 CPU sec, 13.012 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (940, 458725, 796, 925) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (695, 2506, 5477) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15041) [M::mem_pestat] mean and std.dev: (3253.00, 2854.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19823) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 192, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 592) [M::mem_pestat] mean and std.dev: (214.62, 112.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 746) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1312, 3132, 5932) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15172) [M::mem_pestat] mean and std.dev: (3733.52, 2822.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19792) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (617, 2425, 5350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14816) [M::mem_pestat] mean and std.dev: (3212.83, 2845.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19549) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 203.805 CPU sec, 13.024 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (977, 457926, 802, 877) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (726, 2786, 5715) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15693) [M::mem_pestat] mean and std.dev: (3486.19, 2972.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20682) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 191, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591) [M::mem_pestat] mean and std.dev: (214.35, 112.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 745) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1281, 3273, 6265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16233) [M::mem_pestat] mean and std.dev: (3847.22, 2907.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21217) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (748, 2731, 5750) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15754) [M::mem_pestat] mean and std.dev: (3454.44, 2964.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20756) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 203.638 CPU sec, 13.116 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (919, 458367, 810, 888) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (661, 2521, 5566) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15376) [M::mem_pestat] mean and std.dev: (3319.60, 2917.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20281) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 192, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594) [M::mem_pestat] mean and std.dev: (214.63, 112.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 749) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1106, 3067, 5935) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15593) [M::mem_pestat] mean and std.dev: (3728.92, 2927.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20422) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (732, 2544, 5500) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15036) [M::mem_pestat] mean and std.dev: (3376.39, 2968.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19804) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 202.822 CPU sec, 12.905 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (957, 458004, 818, 984) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (748, 2382, 5663) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15493) [M::mem_pestat] mean and std.dev: (3347.37, 2949.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20408) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 192, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 592) [M::mem_pestat] mean and std.dev: (215.05, 113.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 746) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1275, 3380, 5793) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14829) [M::mem_pestat] mean and std.dev: (3818.16, 2854.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19347) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (785, 2405, 5410) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14660) [M::mem_pestat] mean and std.dev: (3344.76, 2951.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19285) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 202.946 CPU sec, 13.271 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (981, 458579, 799, 870) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (671, 2631, 5579) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15395) [M::mem_pestat] mean and std.dev: (3394.12, 2933.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20303) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 192, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 592) [M::mem_pestat] mean and std.dev: (214.97, 112.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 746) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1173, 3164, 6188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16218) [M::mem_pestat] mean and std.dev: (3833.20, 2993.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21233) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (716, 2382, 5076) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13796) [M::mem_pestat] mean and std.dev: (3200.47, 2778.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18156) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 203.845 CPU sec, 12.961 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (868, 457281, 850, 896) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (665, 2283, 5789) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16037) [M::mem_pestat] mean and std.dev: (3347.00, 2978.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21161) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 192, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 592) [M::mem_pestat] mean and std.dev: (214.88, 112.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 746) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1188, 3391, 5907) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15345) [M::mem_pestat] mean and std.dev: (3797.12, 2899.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20064) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (813, 2786, 5706) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15492) [M::mem_pestat] mean and std.dev: (3501.89, 2953.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20385) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 204.306 CPU sec, 13.216 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (898, 458027, 815, 852) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (803, 2762, 5825) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15869) [M::mem_pestat] mean and std.dev: (3488.63, 2966.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20891) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 191, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588) [M::mem_pestat] mean and std.dev: (213.35, 111.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 741) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1246, 2842, 5861) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15091) [M::mem_pestat] mean and std.dev: (3621.26, 2839.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19706) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (822, 2393, 5576) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15084) [M::mem_pestat] mean and std.dev: (3345.94, 2912.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19838) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 202.695 CPU sec, 12.904 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (940, 458205, 832, 914) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (787, 2751, 5615) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15271) [M::mem_pestat] mean and std.dev: (3432.79, 2910.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20099) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 191, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588) [M::mem_pestat] mean and std.dev: (213.87, 112.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 741) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1207, 3189, 6073) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15805) [M::mem_pestat] mean and std.dev: (3795.48, 2951.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20671) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (754, 2595, 5465) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14887) [M::mem_pestat] mean and std.dev: (3335.52, 2871.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19598) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 203.304 CPU sec, 13.122 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (948, 457798, 795, 863) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (730, 2417, 6011) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16573) [M::mem_pestat] mean and std.dev: (3441.06, 3023.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21854) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 190, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588) [M::mem_pestat] mean and std.dev: (213.18, 111.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 741) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1522, 3647, 6126) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15334) [M::mem_pestat] mean and std.dev: (3917.40, 2812.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19938) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (720, 2258, 5421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14823) [M::mem_pestat] mean and std.dev: (3213.65, 2813.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19524) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 203.579 CPU sec, 12.940 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (810, 458461, 847, 911) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (610, 2379, 5376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14908) [M::mem_pestat] mean and std.dev: (3224.16, 2876.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19674) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 190, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588) [M::mem_pestat] mean and std.dev: (213.01, 111.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 741) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1054, 3277, 6380) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 17032) [M::mem_pestat] mean and std.dev: (3832.09, 2975.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 22358) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (801, 2373, 5139) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13815) [M::mem_pestat] mean and std.dev: (3213.53, 2764.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18153) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1333334 sequences (200000100 bp)... [M::mem_process_seqs] Processed 1333334 reads in 204.433 CPU sec, 13.058 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (924, 458921, 809, 915) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (712, 2390, 5392) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14752) [M::mem_pestat] mean and std.dev: (3277.35, 2861.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19432) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 190, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 585) [M::mem_pestat] mean and std.dev: (212.11, 110.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 737) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1037, 2991, 5563) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14615) [M::mem_pestat] mean and std.dev: (3544.76, 2806.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19141) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (667, 2329, 5436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14974) [M::mem_pestat] mean and std.dev: (3249.96, 2928.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19743) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 203.274 CPU sec, 13.046 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (879, 457415, 767, 891) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (671, 2104, 5244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14390) [M::mem_pestat] mean and std.dev: (3178.08, 2883.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18963) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 189, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 581) [M::mem_pestat] mean and std.dev: (211.60, 110.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 732) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1237, 3201, 6288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16390) [M::mem_pestat] mean and std.dev: (3868.08, 2990.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21441) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (788, 2562, 5405) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14639) [M::mem_pestat] mean and std.dev: (3393.98, 2904.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19256) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 199.139 CPU sec, 12.830 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (884, 458653, 745, 899) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (612, 2151, 5113) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14115) [M::mem_pestat] mean and std.dev: (3114.17, 2832.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18616) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 189, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 584) [M::mem_pestat] mean and std.dev: (211.50, 110.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 736) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (957, 3110, 6268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16890) [M::mem_pestat] mean and std.dev: (3751.76, 3008.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 22201) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (814, 2741, 5866) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15970) [M::mem_pestat] mean and std.dev: (3460.93, 2915.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21022) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 196.005 CPU sec, 12.844 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (905, 458401, 791, 880) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (650, 2133, 5207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14321) [M::mem_pestat] mean and std.dev: (3155.80, 2889.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18878) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 189, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 587) [M::mem_pestat] mean and std.dev: (212.44, 111.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 740) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (936, 2784, 5753) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15387) [M::mem_pestat] mean and std.dev: (3566.15, 2912.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20204) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (774, 2608, 5573) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15171) [M::mem_pestat] mean and std.dev: (3391.96, 2864.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19970) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 195.716 CPU sec, 12.664 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (884, 458078, 811, 860) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (659, 2258, 5181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14225) [M::mem_pestat] mean and std.dev: (3188.20, 2866.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18747) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 189, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 587) [M::mem_pestat] mean and std.dev: (212.33, 111.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 740) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1122, 3142, 6322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16722) [M::mem_pestat] mean and std.dev: (3798.87, 2984.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21922) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (760, 2649, 5634) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15382) [M::mem_pestat] mean and std.dev: (3450.19, 2964.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20256) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 195.805 CPU sec, 12.488 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (875, 458802, 757, 938) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (732, 2417, 5345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14571) [M::mem_pestat] mean and std.dev: (3295.45, 2921.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19184) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 189, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 584) [M::mem_pestat] mean and std.dev: (212.18, 110.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 736) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1013, 3330, 6074) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16196) [M::mem_pestat] mean and std.dev: (3809.48, 2972.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21257) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (800, 2441, 5474) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14822) [M::mem_pestat] mean and std.dev: (3332.43, 2895.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19496) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 196.683 CPU sec, 12.745 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (967, 457843, 791, 884) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (767, 2515, 5579) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15203) [M::mem_pestat] mean and std.dev: (3408.34, 2971.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20015) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 192, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595) [M::mem_pestat] mean and std.dev: (215.41, 113.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 750) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1084, 3356, 6367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16933) [M::mem_pestat] mean and std.dev: (3884.78, 3041.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 22216) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (799, 2537, 4952) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13258) [M::mem_pestat] mean and std.dev: (3209.11, 2739.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17411) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 194.334 CPU sec, 12.418 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (957, 458206, 837, 926) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (624, 2319, 5401) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14955) [M::mem_pestat] mean and std.dev: (3202.45, 2853.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19732) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 193, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 598) [M::mem_pestat] mean and std.dev: (216.44, 113.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 754) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1276, 3195, 5964) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15340) [M::mem_pestat] mean and std.dev: (3726.66, 2841.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20028) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (617, 2255, 5786) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16124) [M::mem_pestat] mean and std.dev: (3303.92, 3027.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21293) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 195.601 CPU sec, 12.682 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (988, 457820, 879, 904) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (702, 2341, 5290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14466) [M::mem_pestat] mean and std.dev: (3233.36, 2877.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19054) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 193, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 598) [M::mem_pestat] mean and std.dev: (216.23, 113.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 754) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1343, 3403, 6199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15911) [M::mem_pestat] mean and std.dev: (3950.16, 2957.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20767) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (810, 2401, 6028) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16464) [M::mem_pestat] mean and std.dev: (3442.62, 3006.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21682) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 196.873 CPU sec, 12.648 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (952, 457851, 770, 892) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (868, 2574, 5951) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16117) [M::mem_pestat] mean and std.dev: (3500.32, 2965.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21200) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 193, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 598) [M::mem_pestat] mean and std.dev: (216.27, 113.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 754) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1584, 3500, 6046) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14970) [M::mem_pestat] mean and std.dev: (3930.99, 2794.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19432) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (774, 2647, 5463) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14841) [M::mem_pestat] mean and std.dev: (3340.31, 2835.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19530) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 195.077 CPU sec, 12.658 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (932, 457175, 824, 945) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (757, 2440, 5682) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15532) [M::mem_pestat] mean and std.dev: (3389.64, 2977.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20457) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 193, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 598) [M::mem_pestat] mean and std.dev: (216.53, 114.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 754) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1259, 3016, 6023) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15551) [M::mem_pestat] mean and std.dev: (3736.60, 2933.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20315) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (768, 2492, 5652) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15420) [M::mem_pestat] mean and std.dev: (3315.45, 2861.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20304) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 196.096 CPU sec, 12.649 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (905, 458266, 816, 923) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (726, 2247, 4949) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13395) [M::mem_pestat] mean and std.dev: (3132.15, 2796.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17618) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 193, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 598) [M::mem_pestat] mean and std.dev: (216.05, 113.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 754) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1385, 3826, 6582) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16976) [M::mem_pestat] mean and std.dev: (4131.07, 2987.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 22173) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (643, 2178, 5168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14218) [M::mem_pestat] mean and std.dev: (3130.04, 2832.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18743) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 196.847 CPU sec, 12.759 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (917, 457605, 901, 937) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (747, 2587, 5244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14238) [M::mem_pestat] mean and std.dev: (3273.60, 2812.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18735) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 192, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595) [M::mem_pestat] mean and std.dev: (215.36, 113.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 750) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1031, 3136, 6226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16616) [M::mem_pestat] mean and std.dev: (3791.86, 2998.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21811) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (631, 2252, 5598) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15532) [M::mem_pestat] mean and std.dev: (3277.78, 3006.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20499) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 195.124 CPU sec, 12.513 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (980, 457256, 811, 865) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (701, 2338, 5223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14267) [M::mem_pestat] mean and std.dev: (3181.90, 2774.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18789) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 192, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594) [M::mem_pestat] mean and std.dev: (214.66, 112.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 749) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1107, 2930, 5762) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15072) [M::mem_pestat] mean and std.dev: (3530.90, 2812.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19727) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (763, 2558, 5471) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14887) [M::mem_pestat] mean and std.dev: (3288.75, 2826.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19595) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 193.529 CPU sec, 12.595 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (850, 458543, 824, 900) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (702, 2176, 4980) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13536) [M::mem_pestat] mean and std.dev: (3081.77, 2820.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17814) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 192, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 592) [M::mem_pestat] mean and std.dev: (214.98, 112.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 746) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1282, 2995, 5794) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14818) [M::mem_pestat] mean and std.dev: (3654.44, 2790.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19330) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (720, 2482, 5341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14583) [M::mem_pestat] mean and std.dev: (3292.24, 2836.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19204) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 192.766 CPU sec, 12.500 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (938, 458575, 769, 896) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (739, 2341, 5611) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15355) [M::mem_pestat] mean and std.dev: (3306.29, 2890.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20227) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 192, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595) [M::mem_pestat] mean and std.dev: (215.29, 113.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 750) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1129, 3249, 6093) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16021) [M::mem_pestat] mean and std.dev: (3802.24, 2886.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20985) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (642, 2089, 5005) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13731) [M::mem_pestat] mean and std.dev: (3085.54, 2791.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18094) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 193.889 CPU sec, 12.595 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (935, 458513, 741, 890) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (757, 2425, 5837) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15997) [M::mem_pestat] mean and std.dev: (3396.73, 3007.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21077) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 193, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 598) [M::mem_pestat] mean and std.dev: (216.19, 113.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 754) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1269, 3465, 6154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15924) [M::mem_pestat] mean and std.dev: (3888.95, 2911.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20809) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (741, 2647, 5557) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15189) [M::mem_pestat] mean and std.dev: (3358.20, 2868.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20005) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 195.079 CPU sec, 12.411 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (946, 458018, 833, 859) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (738, 2496, 5510) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15054) [M::mem_pestat] mean and std.dev: (3317.32, 2879.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19826) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 193, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 598) [M::mem_pestat] mean and std.dev: (216.46, 114.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 754) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1136, 3264, 6205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16343) [M::mem_pestat] mean and std.dev: (3854.42, 2960.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21412) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (702, 2543, 5380) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14736) [M::mem_pestat] mean and std.dev: (3362.01, 2931.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19414) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 193.148 CPU sec, 12.528 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (935, 458246, 834, 891) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (799, 2830, 5775) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15727) [M::mem_pestat] mean and std.dev: (3528.94, 2932.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20703) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 192, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595) [M::mem_pestat] mean and std.dev: (215.38, 113.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 750) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1194, 3241, 6394) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16794) [M::mem_pestat] mean and std.dev: (3844.42, 2960.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21994) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (649, 2457, 5409) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14929) [M::mem_pestat] mean and std.dev: (3280.77, 2894.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19689) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 192.166 CPU sec, 12.401 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (957, 457718, 795, 940) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (649, 2251, 5083) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13951) [M::mem_pestat] mean and std.dev: (3118.67, 2783.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18385) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 192, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594) [M::mem_pestat] mean and std.dev: (214.85, 113.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 749) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1165, 3304, 6083) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15919) [M::mem_pestat] mean and std.dev: (3795.45, 2934.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20837) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (788, 2470, 5432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14720) [M::mem_pestat] mean and std.dev: (3270.47, 2798.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19364) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 195.772 CPU sec, 12.714 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (897, 457746, 832, 920) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (641, 2145, 5291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14591) [M::mem_pestat] mean and std.dev: (3232.94, 2917.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19241) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 193, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 598) [M::mem_pestat] mean and std.dev: (216.15, 113.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 754) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1250, 3156, 6214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16142) [M::mem_pestat] mean and std.dev: (3817.89, 2942.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21106) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (777, 2572, 5331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14439) [M::mem_pestat] mean and std.dev: (3366.35, 2895.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18993) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 195.579 CPU sec, 12.673 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (987, 457780, 800, 950) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (745, 2640, 5698) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15604) [M::mem_pestat] mean and std.dev: (3438.98, 2996.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20557) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 192, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595) [M::mem_pestat] mean and std.dev: (215.69, 113.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 750) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1177, 3082, 5852) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15202) [M::mem_pestat] mean and std.dev: (3740.49, 2891.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19877) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (679, 2096, 4962) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13528) [M::mem_pestat] mean and std.dev: (3045.54, 2802.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17811) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 194.350 CPU sec, 12.569 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (966, 458663, 834, 959) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (687, 2363, 5279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14463) [M::mem_pestat] mean and std.dev: (3206.12, 2895.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19055) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 193, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595) [M::mem_pestat] mean and std.dev: (215.65, 113.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 750) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1121, 3010, 5776) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15086) [M::mem_pestat] mean and std.dev: (3599.82, 2761.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19741) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (650, 2294, 5436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15008) [M::mem_pestat] mean and std.dev: (3285.72, 2958.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19794) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 195.830 CPU sec, 12.461 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (963, 457254, 813, 863) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (750, 2435, 5498) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14994) [M::mem_pestat] mean and std.dev: (3290.09, 2906.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19742) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 192, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595) [M::mem_pestat] mean and std.dev: (215.53, 113.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 750) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1317, 3237, 6333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16365) [M::mem_pestat] mean and std.dev: (3837.88, 2867.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21381) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (746, 2485, 5546) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15146) [M::mem_pestat] mean and std.dev: (3339.05, 2911.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19946) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 193.807 CPU sec, 12.558 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (951, 458102, 831, 952) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (743, 2067, 4684) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12566) [M::mem_pestat] mean and std.dev: (2965.64, 2691.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16507) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 191, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 589) [M::mem_pestat] mean and std.dev: (214.05, 112.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 742) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1116, 3017, 5739) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14985) [M::mem_pestat] mean and std.dev: (3662.73, 2852.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19608) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (711, 2417, 5183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14127) [M::mem_pestat] mean and std.dev: (3293.21, 2853.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18599) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 192.596 CPU sec, 12.396 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (925, 457823, 802, 931) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (616, 2303, 5446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15106) [M::mem_pestat] mean and std.dev: (3194.25, 2838.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19936) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 191, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591) [M::mem_pestat] mean and std.dev: (213.90, 112.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 745) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1138, 3105, 5832) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15220) [M::mem_pestat] mean and std.dev: (3738.60, 2897.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19914) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (676, 2397, 5399) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14845) [M::mem_pestat] mean and std.dev: (3238.20, 2871.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19568) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 193.798 CPU sec, 12.562 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (902, 457753, 883, 877) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (831, 2629, 5852) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15894) [M::mem_pestat] mean and std.dev: (3486.94, 2998.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20915) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 191, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591) [M::mem_pestat] mean and std.dev: (214.08, 112.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 745) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1230, 2902, 5755) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14805) [M::mem_pestat] mean and std.dev: (3581.54, 2757.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19330) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (681, 2506, 5834) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16140) [M::mem_pestat] mean and std.dev: (3376.05, 2950.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21293) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 194.426 CPU sec, 12.653 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (884, 457800, 810, 861) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (629, 2212, 5309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14669) [M::mem_pestat] mean and std.dev: (3170.67, 2867.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19349) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 190, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588) [M::mem_pestat] mean and std.dev: (213.40, 111.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 741) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1062, 2689, 5610) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14706) [M::mem_pestat] mean and std.dev: (3509.83, 2860.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19254) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (700, 2458, 5325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14575) [M::mem_pestat] mean and std.dev: (3279.80, 2863.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19200) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 192.079 CPU sec, 12.473 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (849, 458197, 741, 855) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (668, 2355, 5211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14297) [M::mem_pestat] mean and std.dev: (3148.01, 2805.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18840) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 190, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 585) [M::mem_pestat] mean and std.dev: (212.33, 111.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 737) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1260, 3024, 6113) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15819) [M::mem_pestat] mean and std.dev: (3716.06, 2887.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20672) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (689, 2427, 5089) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13889) [M::mem_pestat] mean and std.dev: (3230.71, 2836.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18289) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 193.138 CPU sec, 12.485 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (895, 457243, 791, 872) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (762, 2786, 5691) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15549) [M::mem_pestat] mean and std.dev: (3421.72, 2884.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20478) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 189, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 584) [M::mem_pestat] mean and std.dev: (211.99, 110.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 736) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1200, 3184, 6185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16155) [M::mem_pestat] mean and std.dev: (3817.37, 2903.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21140) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (637, 2459, 5380) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14866) [M::mem_pestat] mean and std.dev: (3293.26, 2920.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19609) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 193.467 CPU sec, 12.538 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (936, 458220, 833, 896) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (722, 2498, 5312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14492) [M::mem_pestat] mean and std.dev: (3275.44, 2872.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19082) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 190, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 584) [M::mem_pestat] mean and std.dev: (212.08, 110.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 736) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1155, 3094, 6141) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16113) [M::mem_pestat] mean and std.dev: (3723.09, 2902.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21099) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (738, 2579, 5203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14133) [M::mem_pestat] mean and std.dev: (3211.99, 2764.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18598) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 193.522 CPU sec, 12.493 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (948, 458689, 771, 966) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (765, 2495, 5402) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14676) [M::mem_pestat] mean and std.dev: (3270.63, 2832.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19313) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 189, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 585) [M::mem_pestat] mean and std.dev: (212.26, 110.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 737) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1124, 3101, 5967) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15653) [M::mem_pestat] mean and std.dev: (3658.73, 2842.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20496) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (761, 2216, 5099) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13775) [M::mem_pestat] mean and std.dev: (3256.65, 2897.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18113) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 195.054 CPU sec, 12.763 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (946, 458227, 805, 858) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (651, 2406, 5522) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15264) [M::mem_pestat] mean and std.dev: (3272.60, 2887.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20135) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 190, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 585) [M::mem_pestat] mean and std.dev: (212.82, 111.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 737) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1038, 3029, 6038) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16038) [M::mem_pestat] mean and std.dev: (3698.44, 2907.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21038) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (606, 2687, 5806) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16206) [M::mem_pestat] mean and std.dev: (3386.90, 3002.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21406) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 193.854 CPU sec, 12.333 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (953, 457944, 787, 915) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (643, 2386, 5217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14365) [M::mem_pestat] mean and std.dev: (3164.29, 2802.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18939) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 190, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 585) [M::mem_pestat] mean and std.dev: (212.41, 111.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 737) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1118, 3137, 5810) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15194) [M::mem_pestat] mean and std.dev: (3628.52, 2866.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19886) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (762, 2594, 5733) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15675) [M::mem_pestat] mean and std.dev: (3395.85, 2900.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20646) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 194.357 CPU sec, 12.655 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (971, 457548, 813, 975) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (694, 2558, 5897) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16303) [M::mem_pestat] mean and std.dev: (3420.85, 2943.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21506) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 193, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 596) [M::mem_pestat] mean and std.dev: (216.48, 113.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 751) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1038, 2690, 5769) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15231) [M::mem_pestat] mean and std.dev: (3610.42, 2970.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19962) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (703, 2394, 5266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14392) [M::mem_pestat] mean and std.dev: (3269.33, 2919.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18955) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 207.233 CPU sec, 13.468 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (950, 457979, 811, 968) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (867, 2837, 5576) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14994) [M::mem_pestat] mean and std.dev: (3497.00, 2897.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19703) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 193, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 598) [M::mem_pestat] mean and std.dev: (216.39, 114.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 754) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1112, 3175, 5984) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15728) [M::mem_pestat] mean and std.dev: (3792.53, 2921.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20600) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (714, 2286, 5017) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13623) [M::mem_pestat] mean and std.dev: (3140.57, 2801.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17926) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 195.702 CPU sec, 12.692 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (886, 457647, 795, 867) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (732, 2391, 5303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14445) [M::mem_pestat] mean and std.dev: (3163.21, 2789.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19016) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 193, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 598) [M::mem_pestat] mean and std.dev: (216.50, 114.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 754) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1250, 3309, 6242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16226) [M::mem_pestat] mean and std.dev: (3851.06, 2936.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21218) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (763, 2544, 5765) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15769) [M::mem_pestat] mean and std.dev: (3427.30, 2977.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20771) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 196.254 CPU sec, 12.490 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (906, 458278, 857, 958) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (698, 2741, 5814) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16046) [M::mem_pestat] mean and std.dev: (3490.11, 3005.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21162) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 193, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595) [M::mem_pestat] mean and std.dev: (215.80, 113.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 750) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1223, 3131, 6039) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15671) [M::mem_pestat] mean and std.dev: (3788.13, 2859.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20487) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (771, 2602, 5439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14775) [M::mem_pestat] mean and std.dev: (3393.77, 2886.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19443) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 194.413 CPU sec, 12.577 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (869, 458361, 797, 871) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (573, 2071, 5308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14778) [M::mem_pestat] mean and std.dev: (3080.80, 2829.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19513) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 193, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 598) [M::mem_pestat] mean and std.dev: (216.26, 113.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 754) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1094, 3158, 6174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16334) [M::mem_pestat] mean and std.dev: (3754.37, 2933.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21414) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (831, 2625, 5455) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14703) [M::mem_pestat] mean and std.dev: (3371.92, 2887.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19327) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 196.695 CPU sec, 12.864 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (951, 457355, 814, 934) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (753, 2481, 5490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14964) [M::mem_pestat] mean and std.dev: (3333.73, 2925.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19701) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 193, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 598) [M::mem_pestat] mean and std.dev: (216.33, 113.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 754) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1141, 3199, 6136) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16126) [M::mem_pestat] mean and std.dev: (3769.12, 2907.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21121) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (799, 2727, 5618) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15256) [M::mem_pestat] mean and std.dev: (3465.93, 2880.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20075) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 195.220 CPU sec, 12.650 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (923, 458056, 795, 932) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (663, 2334, 5297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14565) [M::mem_pestat] mean and std.dev: (3202.00, 2852.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19199) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 193, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 598) [M::mem_pestat] mean and std.dev: (216.20, 113.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 754) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1263, 3369, 6398) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16668) [M::mem_pestat] mean and std.dev: (3885.90, 2926.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21803) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (718, 2509, 5515) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15109) [M::mem_pestat] mean and std.dev: (3325.61, 2887.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19906) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 194.956 CPU sec, 12.393 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (987, 458295, 861, 969) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (749, 2641, 5572) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15218) [M::mem_pestat] mean and std.dev: (3360.99, 2887.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20041) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 192, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595) [M::mem_pestat] mean and std.dev: (215.34, 113.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 750) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1034, 3351, 6247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16673) [M::mem_pestat] mean and std.dev: (3840.45, 3015.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21886) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (716, 2363, 5190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14138) [M::mem_pestat] mean and std.dev: (3278.76, 2906.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18612) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 192.797 CPU sec, 12.500 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (842, 457479, 774, 924) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (667, 2353, 5364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14758) [M::mem_pestat] mean and std.dev: (3221.92, 2886.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19455) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 192, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595) [M::mem_pestat] mean and std.dev: (215.75, 113.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 750) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1078, 3006, 5810) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15274) [M::mem_pestat] mean and std.dev: (3651.32, 2925.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20006) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (654, 2478, 5579) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15429) [M::mem_pestat] mean and std.dev: (3326.51, 2927.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20354) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 194.942 CPU sec, 12.548 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (901, 458869, 758, 892) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (652, 2216, 5175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14221) [M::mem_pestat] mean and std.dev: (3197.68, 2908.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18744) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 193, 287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 601) [M::mem_pestat] mean and std.dev: (216.78, 114.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 758) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1324, 3239, 5793) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14731) [M::mem_pestat] mean and std.dev: (3782.36, 2886.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19200) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (791, 2856, 6009) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16445) [M::mem_pestat] mean and std.dev: (3588.93, 3014.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21663) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 194.969 CPU sec, 12.620 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (944, 459114, 789, 950) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (735, 2590, 5417) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14781) [M::mem_pestat] mean and std.dev: (3358.67, 2922.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19463) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 193, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595) [M::mem_pestat] mean and std.dev: (215.79, 113.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 750) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1117, 3188, 5965) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15661) [M::mem_pestat] mean and std.dev: (3744.88, 2934.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20509) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (647, 1969, 5196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14294) [M::mem_pestat] mean and std.dev: (3115.49, 2916.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18843) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 194.366 CPU sec, 12.647 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (939, 457835, 826, 938) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (741, 2603, 6095) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16803) [M::mem_pestat] mean and std.dev: (3482.46, 3018.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 22157) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 193, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 598) [M::mem_pestat] mean and std.dev: (216.67, 114.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 754) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1272, 3214, 6098) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15750) [M::mem_pestat] mean and std.dev: (3814.47, 2855.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20576) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (649, 2339, 5315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14647) [M::mem_pestat] mean and std.dev: (3204.28, 2852.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19313) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 195.122 CPU sec, 12.650 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (920, 458129, 836, 907) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (684, 2577, 5618) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15486) [M::mem_pestat] mean and std.dev: (3393.15, 2995.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20420) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 193, 287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 601) [M::mem_pestat] mean and std.dev: (216.93, 114.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 758) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1222, 3348, 6249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16303) [M::mem_pestat] mean and std.dev: (3911.11, 2969.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21330) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (731, 2473, 5801) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15941) [M::mem_pestat] mean and std.dev: (3375.95, 2955.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21011) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 193.902 CPU sec, 12.665 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (934, 458494, 787, 932) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (684, 2393, 5334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14634) [M::mem_pestat] mean and std.dev: (3267.38, 2934.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19284) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 193, 287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 601) [M::mem_pestat] mean and std.dev: (216.92, 114.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 758) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1129, 3212, 6199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16339) [M::mem_pestat] mean and std.dev: (3851.29, 2962.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21409) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (828, 2579, 5424) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14616) [M::mem_pestat] mean and std.dev: (3376.90, 2853.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19212) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 193.935 CPU sec, 12.576 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (896, 458122, 818, 885) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (667, 2217, 5319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14623) [M::mem_pestat] mean and std.dev: (3250.91, 2925.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19275) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 193, 287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 601) [M::mem_pestat] mean and std.dev: (217.07, 114.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 758) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1041, 2829, 5864) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15510) [M::mem_pestat] mean and std.dev: (3579.44, 2903.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20333) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (769, 2558, 5546) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15100) [M::mem_pestat] mean and std.dev: (3422.09, 2925.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19877) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 194.450 CPU sec, 12.393 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (927, 458107, 817, 941) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (812, 2581, 5560) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15056) [M::mem_pestat] mean and std.dev: (3456.10, 2974.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19804) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 193, 287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 601) [M::mem_pestat] mean and std.dev: (217.00, 114.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 758) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1199, 3362, 6027) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15683) [M::mem_pestat] mean and std.dev: (3832.21, 2912.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20511) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (628, 2268, 5189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14311) [M::mem_pestat] mean and std.dev: (3169.42, 2831.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18872) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 197.251 CPU sec, 12.753 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (993, 458496, 796, 962) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (690, 2331, 5563) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15309) [M::mem_pestat] mean and std.dev: (3306.05, 2951.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20182) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 193, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 598) [M::mem_pestat] mean and std.dev: (216.46, 114.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 754) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1304, 3361, 6214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16034) [M::mem_pestat] mean and std.dev: (3836.35, 2853.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20944) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (893, 2755, 5527) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14795) [M::mem_pestat] mean and std.dev: (3473.50, 2859.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19429) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 195.985 CPU sec, 12.665 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (945, 457839, 785, 922) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (582, 2265, 5269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14643) [M::mem_pestat] mean and std.dev: (3180.88, 2911.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19330) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 193, 287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 601) [M::mem_pestat] mean and std.dev: (216.78, 114.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 758) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1090, 3139, 6044) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15952) [M::mem_pestat] mean and std.dev: (3716.07, 2902.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20906) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (686, 2298, 5508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15152) [M::mem_pestat] mean and std.dev: (3263.25, 2932.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19974) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 194.432 CPU sec, 12.584 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (996, 457514, 814, 958) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (779, 2545, 5803) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15851) [M::mem_pestat] mean and std.dev: (3451.14, 2969.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20875) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 192, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595) [M::mem_pestat] mean and std.dev: (215.41, 113.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 750) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1139, 3002, 5766) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15020) [M::mem_pestat] mean and std.dev: (3699.37, 2889.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19647) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (757, 2378, 5867) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16087) [M::mem_pestat] mean and std.dev: (3424.07, 3025.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21197) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 194.059 CPU sec, 12.614 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (874, 458020, 786, 912) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (676, 2248, 5388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14812) [M::mem_pestat] mean and std.dev: (3213.61, 2893.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19524) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 192, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595) [M::mem_pestat] mean and std.dev: (215.41, 113.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 750) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1136, 2938, 6051) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15881) [M::mem_pestat] mean and std.dev: (3672.48, 2881.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20796) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (899, 2616, 5380) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14342) [M::mem_pestat] mean and std.dev: (3381.17, 2795.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18823) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 194.305 CPU sec, 12.595 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (880, 458019, 812, 875) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (717, 2746, 5658) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15540) [M::mem_pestat] mean and std.dev: (3408.18, 2965.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20481) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 192, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594) [M::mem_pestat] mean and std.dev: (214.82, 112.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 749) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1032, 2699, 5637) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14847) [M::mem_pestat] mean and std.dev: (3466.84, 2837.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19452) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (694, 2616, 5728) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15796) [M::mem_pestat] mean and std.dev: (3404.64, 2974.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20830) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 192.080 CPU sec, 12.409 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (860, 458393, 768, 987) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (698, 2428, 5395) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14789) [M::mem_pestat] mean and std.dev: (3304.60, 2927.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19486) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 191, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591) [M::mem_pestat] mean and std.dev: (214.23, 112.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 745) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1128, 3099, 6005) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15759) [M::mem_pestat] mean and std.dev: (3733.90, 2852.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20636) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (814, 2346, 5539) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14989) [M::mem_pestat] mean and std.dev: (3356.01, 2922.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19714) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 194.347 CPU sec, 12.577 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (872, 458000, 832, 900) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (834, 2471, 5565) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15027) [M::mem_pestat] mean and std.dev: (3398.11, 2929.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19758) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 191, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591) [M::mem_pestat] mean and std.dev: (213.80, 112.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 745) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1243, 3205, 6022) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15580) [M::mem_pestat] mean and std.dev: (3803.81, 2853.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20359) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (671, 2343, 5290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14528) [M::mem_pestat] mean and std.dev: (3205.50, 2806.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19147) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 194.657 CPU sec, 12.547 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (943, 457933, 826, 917) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (693, 2257, 4968) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13518) [M::mem_pestat] mean and std.dev: (3141.80, 2811.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17793) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 190, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588) [M::mem_pestat] mean and std.dev: (213.20, 111.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 741) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1054, 3255, 6007) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15913) [M::mem_pestat] mean and std.dev: (3728.96, 2906.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20866) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (797, 2635, 5815) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15851) [M::mem_pestat] mean and std.dev: (3436.91, 2924.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20869) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 196.632 CPU sec, 12.763 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (898, 458725, 809, 903) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (736, 2356, 5349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14575) [M::mem_pestat] mean and std.dev: (3244.18, 2877.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19188) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 191, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591) [M::mem_pestat] mean and std.dev: (213.93, 112.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 745) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1159, 3139, 5755) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14947) [M::mem_pestat] mean and std.dev: (3719.21, 2860.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19543) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (707, 2636, 5682) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15632) [M::mem_pestat] mean and std.dev: (3383.53, 2894.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20607) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 194.776 CPU sec, 12.392 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (922, 457573, 812, 980) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (612, 2307, 5335) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14781) [M::mem_pestat] mean and std.dev: (3261.66, 2966.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19504) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 191, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591) [M::mem_pestat] mean and std.dev: (213.95, 112.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 745) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1097, 3008, 6250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16556) [M::mem_pestat] mean and std.dev: (3738.08, 2921.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21709) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (730, 2396, 5379) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14677) [M::mem_pestat] mean and std.dev: (3288.04, 2941.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19326) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 195.815 CPU sec, 12.686 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (927, 458400, 755, 882) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (734, 2447, 5026) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13610) [M::mem_pestat] mean and std.dev: (3162.65, 2765.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17902) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 190, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588) [M::mem_pestat] mean and std.dev: (213.47, 112.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 741) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1047, 2867, 5443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14235) [M::mem_pestat] mean and std.dev: (3495.29, 2773.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18631) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (664, 1974, 5232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14368) [M::mem_pestat] mean and std.dev: (3076.51, 2865.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18936) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 195.592 CPU sec, 12.432 real sec [M::process] read 688510 sequences (103276500 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (449, 236428, 411, 469) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (594, 2215, 5661) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15795) [M::mem_pestat] mean and std.dev: (3226.96, 2964.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20862) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 191, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 589) [M::mem_pestat] mean and std.dev: (214.09, 111.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 742) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1491, 3189, 6027) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15099) [M::mem_pestat] mean and std.dev: (3815.97, 2841.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19635) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (759, 2346, 5757) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15753) [M::mem_pestat] mean and std.dev: (3443.05, 2979.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20751) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 688510 reads in 100.982 CPU sec, 6.585 real sec [main] Version: 0.7.17-r1188 [main] CMD: bwa mem -SP5M -t 20 /oak/stanford/groups/akundaje/marinovg/ENCODE4/datasets-Hi-C/L1027-Tetrahymena_thermophila_Hi-C_MluCl/juicer-L1027-Tetrahymena_thermophila_Hi-C_MluCl-all-reads-3D_DNA/final-post-review/bwa-indexes/Tetrahymena_thermophila.JCVI-TTA1-2.2.20.FINAL.fasta /oak/stanford/groups/akundaje/marinovg/papers/2022_Tetrahymena-Hi-C/2022-05-24-figures/juicer-L1026-L1027-L1216-L1624-MAC_final_post_review/splits/L1026-L1027-L1216-L1624_R1.fastq.gz /oak/stanford/groups/akundaje/marinovg/papers/2022_Tetrahymena-Hi-C/2022-05-24-figures/juicer-L1026-L1027-L1216-L1624-MAC_final_post_review/splits/L1026-L1027-L1216-L1624_R2.fastq.gz [main] Real time: 11142.318 sec; CPU: 131394.090 sec Picked up _JAVA_OPTIONS: -Xmx16384m Iterated 1000 times, returning estimate Picked up _JAVA_OPTIONS: -Xmx16384m Picked up _JAVA_OPTIONS: -Xmx16384m Picked up _JAVA_OPTIONS: -Xmx16384m