[M::bwa_idx_load_from_disk] read 0 ALT contigs [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8796, 216751, 7773, 8536) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1190, 2964, 5445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13955) [M::mem_pestat] mean and std.dev: (3536.54, 2714.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18210) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (218, 296, 387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 725) [M::mem_pestat] mean and std.dev: (296.35, 121.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 894) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1457, 3177, 5747) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14327) [M::mem_pestat] mean and std.dev: (3754.59, 2740.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18617) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1369, 3026, 5533) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13861) [M::mem_pestat] mean and std.dev: (3645.45, 2681.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18025) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 433.704 CPU sec, 59.169 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8774, 219924, 7766, 8527) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1254, 3006, 5501) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13995) [M::mem_pestat] mean and std.dev: (3581.27, 2699.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18242) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (222, 299, 389) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723) [M::mem_pestat] mean and std.dev: (299.25, 120.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 890) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1510, 3273, 5694) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14062) [M::mem_pestat] mean and std.dev: (3770.90, 2684.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18246) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1326, 3027, 5575) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14073) [M::mem_pestat] mean and std.dev: (3636.10, 2704.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18322) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 354.916 CPU sec, 22.912 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8649, 220284, 7835, 8517) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1248, 2920, 5404) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13716) [M::mem_pestat] mean and std.dev: (3520.19, 2675.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17872) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (223, 300, 390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 724) [M::mem_pestat] mean and std.dev: (301.15, 120.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 891) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1495, 3244, 5677) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14041) [M::mem_pestat] mean and std.dev: (3768.30, 2707.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18223) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1302, 3052, 5644) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14328) [M::mem_pestat] mean and std.dev: (3659.21, 2714.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18670) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 356.872 CPU sec, 22.900 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8740, 218446, 7815, 8412) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1216, 2898, 5455) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13933) [M::mem_pestat] mean and std.dev: (3544.70, 2723.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18172) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (224, 300, 390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 722) [M::mem_pestat] mean and std.dev: (301.04, 119.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 888) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1528, 3302, 5717) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14095) [M::mem_pestat] mean and std.dev: (3811.31, 2691.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18284) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1344, 3031, 5537) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13923) [M::mem_pestat] mean and std.dev: (3644.67, 2690.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18116) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 366.209 CPU sec, 23.477 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8916, 217863, 7922, 8715) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1235, 2944, 5524) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14102) [M::mem_pestat] mean and std.dev: (3583.91, 2745.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18391) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (225, 301, 393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729) [M::mem_pestat] mean and std.dev: (303.30, 120.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 897) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1463, 3215, 5725) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14249) [M::mem_pestat] mean and std.dev: (3771.12, 2736.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18511) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1302, 2955, 5538) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14010) [M::mem_pestat] mean and std.dev: (3610.90, 2701.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18246) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1333334 sequences (200000100 bp)... [M::mem_process_seqs] Processed 1333334 reads in 354.463 CPU sec, 22.585 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8785, 216929, 7891, 8506) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1282, 3012, 5540) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14056) [M::mem_pestat] mean and std.dev: (3604.93, 2722.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18314) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (226, 303, 394) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 730) [M::mem_pestat] mean and std.dev: (304.43, 120.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 898) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1514, 3329, 5732) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14168) [M::mem_pestat] mean and std.dev: (3821.05, 2704.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18386) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1355, 2989, 5542) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13916) [M::mem_pestat] mean and std.dev: (3648.77, 2715.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18103) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 367.228 CPU sec, 23.433 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8554, 219171, 7778, 8549) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1279, 3081, 5536) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14050) [M::mem_pestat] mean and std.dev: (3619.79, 2712.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18307) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (227, 303, 394) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 728) [M::mem_pestat] mean and std.dev: (304.52, 120.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 895) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1542, 3325, 5769) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14223) [M::mem_pestat] mean and std.dev: (3835.66, 2708.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18450) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1379, 3131, 5619) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14099) [M::mem_pestat] mean and std.dev: (3713.43, 2731.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18339) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 344.965 CPU sec, 22.001 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8794, 217572, 7833, 8619) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1307, 3015, 5602) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14192) [M::mem_pestat] mean and std.dev: (3638.10, 2740.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18487) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (227, 304, 396) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 734) [M::mem_pestat] mean and std.dev: (306.37, 120.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 903) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1471, 3217, 5632) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13954) [M::mem_pestat] mean and std.dev: (3752.51, 2704.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18115) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1341, 3142, 5601) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14121) [M::mem_pestat] mean and std.dev: (3692.41, 2719.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18381) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 347.064 CPU sec, 22.236 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8759, 217032, 7756, 8500) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1219, 3011, 5524) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14134) [M::mem_pestat] mean and std.dev: (3596.21, 2734.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18439) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (229, 304, 396) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 730) [M::mem_pestat] mean and std.dev: (306.73, 120.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 897) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1507, 3317, 5785) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14341) [M::mem_pestat] mean and std.dev: (3825.85, 2723.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18619) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1342, 3098, 5606) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14134) [M::mem_pestat] mean and std.dev: (3667.65, 2708.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18398) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 359.896 CPU sec, 22.933 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8854, 217642, 7769, 8679) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1190, 2970, 5502) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14126) [M::mem_pestat] mean and std.dev: (3570.79, 2726.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18438) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (228, 304, 397) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735) [M::mem_pestat] mean and std.dev: (306.33, 121.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 904) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1509, 3272, 5796) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14370) [M::mem_pestat] mean and std.dev: (3813.74, 2723.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18657) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1429, 3155, 5640) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14062) [M::mem_pestat] mean and std.dev: (3731.37, 2705.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18273) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 356.212 CPU sec, 22.652 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8698, 217418, 7840, 8622) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1240, 3004, 5584) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14272) [M::mem_pestat] mean and std.dev: (3600.47, 2723.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18616) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (228, 305, 396) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732) [M::mem_pestat] mean and std.dev: (306.91, 120.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 900) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1527, 3355, 5791) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14319) [M::mem_pestat] mean and std.dev: (3835.70, 2703.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18583) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1408, 3141, 5514) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13726) [M::mem_pestat] mean and std.dev: (3680.68, 2685.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17832) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 360.452 CPU sec, 22.949 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8805, 217368, 7779, 8573) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1234, 2970, 5568) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14236) [M::mem_pestat] mean and std.dev: (3586.45, 2725.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18570) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (229, 306, 399) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739) [M::mem_pestat] mean and std.dev: (307.94, 121.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 909) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1464, 3368, 5944) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14904) [M::mem_pestat] mean and std.dev: (3876.30, 2757.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19384) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1355, 3187, 5500) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13790) [M::mem_pestat] mean and std.dev: (3658.16, 2657.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17935) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 345.295 CPU sec, 21.977 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8842, 218327, 7805, 8663) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1186, 2892, 5440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13948) [M::mem_pestat] mean and std.dev: (3530.50, 2724.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18202) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (228, 305, 399) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741) [M::mem_pestat] mean and std.dev: (307.19, 122.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 912) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1504, 3288, 5891) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14665) [M::mem_pestat] mean and std.dev: (3843.80, 2745.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19052) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1378, 3109, 5625) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14119) [M::mem_pestat] mean and std.dev: (3684.61, 2697.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18366) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 345.523 CPU sec, 22.111 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8823, 217689, 7818, 8473) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1223, 3009, 5525) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14129) [M::mem_pestat] mean and std.dev: (3605.63, 2727.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18431) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (228, 305, 399) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741) [M::mem_pestat] mean and std.dev: (307.93, 122.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 912) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1498, 3271, 5716) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14152) [M::mem_pestat] mean and std.dev: (3801.64, 2725.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18370) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1393, 3139, 5553) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13873) [M::mem_pestat] mean and std.dev: (3689.29, 2694.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18033) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 347.067 CPU sec, 22.103 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8761, 216684, 7906, 8675) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1254, 3092, 5598) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14286) [M::mem_pestat] mean and std.dev: (3636.01, 2750.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18630) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (229, 306, 399) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739) [M::mem_pestat] mean and std.dev: (308.27, 121.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 909) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1484, 3313, 5722) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14198) [M::mem_pestat] mean and std.dev: (3789.13, 2699.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18436) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1379, 3117, 5579) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13979) [M::mem_pestat] mean and std.dev: (3687.75, 2712.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18179) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 364.951 CPU sec, 23.234 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8775, 217557, 7873, 8604) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1252, 2958, 5466) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13894) [M::mem_pestat] mean and std.dev: (3561.98, 2703.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18108) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (229, 306, 400) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742) [M::mem_pestat] mean and std.dev: (308.45, 122.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 913) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1495, 3300, 5715) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14155) [M::mem_pestat] mean and std.dev: (3811.48, 2728.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18375) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1330, 3012, 5587) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14101) [M::mem_pestat] mean and std.dev: (3643.29, 2707.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18358) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 346.322 CPU sec, 22.041 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8880, 217624, 7939, 8464) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1276, 3024, 5447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13789) [M::mem_pestat] mean and std.dev: (3595.61, 2726.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17960) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (229, 307, 401) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745) [M::mem_pestat] mean and std.dev: (309.29, 122.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 917) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1502, 3285, 5761) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14279) [M::mem_pestat] mean and std.dev: (3828.95, 2739.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18538) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1339, 3126, 5630) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14212) [M::mem_pestat] mean and std.dev: (3683.30, 2706.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18503) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 345.633 CPU sec, 22.043 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8751, 217815, 7708, 8633) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1196, 2953, 5436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13916) [M::mem_pestat] mean and std.dev: (3556.07, 2735.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18156) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (222, 299, 391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729) [M::mem_pestat] mean and std.dev: (300.52, 122.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 898) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1520, 3254, 5724) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14132) [M::mem_pestat] mean and std.dev: (3803.65, 2708.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18336) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1423, 3097, 5598) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13948) [M::mem_pestat] mean and std.dev: (3691.35, 2688.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18123) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 362.233 CPU sec, 23.022 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8782, 218068, 7783, 8465) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1230, 2984, 5554) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14202) [M::mem_pestat] mean and std.dev: (3597.00, 2740.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18526) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (221, 298, 387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719) [M::mem_pestat] mean and std.dev: (298.58, 119.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 885) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1535, 3335, 5757) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14201) [M::mem_pestat] mean and std.dev: (3823.75, 2704.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18423) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1353, 3028, 5541) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13917) [M::mem_pestat] mean and std.dev: (3645.20, 2700.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18105) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 385.907 CPU sec, 24.609 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8768, 221197, 7742, 8608) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1203, 2915, 5505) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14109) [M::mem_pestat] mean and std.dev: (3568.69, 2735.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18411) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (223, 298, 388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 718) [M::mem_pestat] mean and std.dev: (299.80, 118.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 883) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1530, 3327, 5702) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14046) [M::mem_pestat] mean and std.dev: (3825.75, 2700.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18218) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1405, 3165, 5623) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14059) [M::mem_pestat] mean and std.dev: (3692.56, 2690.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18277) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 369.964 CPU sec, 23.527 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8714, 221235, 7706, 8534) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1250, 3018, 5636) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14408) [M::mem_pestat] mean and std.dev: (3622.29, 2732.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18794) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (224, 299, 388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716) [M::mem_pestat] mean and std.dev: (300.63, 118.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 880) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1540, 3370, 5831) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14413) [M::mem_pestat] mean and std.dev: (3868.26, 2721.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18704) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1365, 3087, 5588) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14034) [M::mem_pestat] mean and std.dev: (3658.05, 2678.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18257) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 364.285 CPU sec, 23.188 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8725, 218779, 7674, 8776) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1292, 3079, 5491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13889) [M::mem_pestat] mean and std.dev: (3615.15, 2702.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18088) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (225, 301, 391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723) [M::mem_pestat] mean and std.dev: (302.17, 118.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 889) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1524, 3348, 5883) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14601) [M::mem_pestat] mean and std.dev: (3859.58, 2725.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18960) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1405, 3155, 5635) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14095) [M::mem_pestat] mean and std.dev: (3718.98, 2704.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18325) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 366.412 CPU sec, 23.359 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8760, 217500, 7913, 8686) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1246, 2985, 5477) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13939) [M::mem_pestat] mean and std.dev: (3577.84, 2709.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18170) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (226, 301, 391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 721) [M::mem_pestat] mean and std.dev: (303.11, 118.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 886) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1484, 3234, 5683) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14081) [M::mem_pestat] mean and std.dev: (3770.94, 2693.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18280) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1360, 3081, 5527) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13861) [M::mem_pestat] mean and std.dev: (3660.05, 2693.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18028) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 378.168 CPU sec, 24.177 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8932, 220737, 7842, 8501) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1215, 2984, 5520) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14130) [M::mem_pestat] mean and std.dev: (3583.48, 2724.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18435) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (226, 302, 393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 727) [M::mem_pestat] mean and std.dev: (303.92, 119.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 894) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1526, 3279, 5715) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14093) [M::mem_pestat] mean and std.dev: (3805.94, 2704.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18282) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1294, 3016, 5531) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14005) [M::mem_pestat] mean and std.dev: (3613.55, 2707.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18242) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 350.024 CPU sec, 22.313 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8764, 218206, 7874, 8586) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1260, 3054, 5445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13815) [M::mem_pestat] mean and std.dev: (3592.28, 2719.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18000) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (227, 302, 393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 725) [M::mem_pestat] mean and std.dev: (304.47, 119.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 891) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1501, 3295, 5806) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14416) [M::mem_pestat] mean and std.dev: (3824.99, 2748.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18721) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1333, 3045, 5488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13798) [M::mem_pestat] mean and std.dev: (3638.68, 2692.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17953) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 363.162 CPU sec, 23.403 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8817, 219053, 7691, 8630) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1268, 3023, 5570) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14174) [M::mem_pestat] mean and std.dev: (3624.14, 2732.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18476) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (228, 302, 393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723) [M::mem_pestat] mean and std.dev: (304.65, 118.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 888) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1476, 3277, 5744) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14280) [M::mem_pestat] mean and std.dev: (3788.94, 2719.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18548) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1348, 3088, 5559) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13981) [M::mem_pestat] mean and std.dev: (3661.70, 2700.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18192) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 362.713 CPU sec, 23.092 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8775, 218631, 7793, 8714) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1208, 3001, 5571) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14297) [M::mem_pestat] mean and std.dev: (3586.62, 2736.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18660) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (228, 303, 394) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726) [M::mem_pestat] mean and std.dev: (305.18, 118.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 892) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1498, 3240, 5608) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13828) [M::mem_pestat] mean and std.dev: (3764.43, 2690.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17938) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1387, 3077, 5565) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13921) [M::mem_pestat] mean and std.dev: (3672.90, 2695.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18099) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 365.423 CPU sec, 23.329 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8912, 218104, 7801, 8551) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1276, 2966, 5453) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13807) [M::mem_pestat] mean and std.dev: (3565.98, 2709.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17984) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (229, 304, 395) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 727) [M::mem_pestat] mean and std.dev: (305.81, 119.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 893) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1507, 3277, 5708) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14110) [M::mem_pestat] mean and std.dev: (3794.27, 2691.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18311) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1395, 3090, 5641) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14133) [M::mem_pestat] mean and std.dev: (3692.69, 2713.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18379) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 365.757 CPU sec, 23.316 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8870, 219870, 7842, 8663) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1266, 2982, 5562) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14154) [M::mem_pestat] mean and std.dev: (3595.26, 2713.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18450) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (228, 304, 395) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729) [M::mem_pestat] mean and std.dev: (305.81, 119.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1505, 3340, 5817) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14441) [M::mem_pestat] mean and std.dev: (3811.30, 2712.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18753) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1337, 3046, 5462) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13712) [M::mem_pestat] mean and std.dev: (3625.40, 2678.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17837) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 352.469 CPU sec, 22.499 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8753, 219003, 7827, 8541) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1235, 3043, 5549) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14177) [M::mem_pestat] mean and std.dev: (3612.72, 2735.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18491) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (228, 304, 396) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732) [M::mem_pestat] mean and std.dev: (306.17, 119.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 900) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1492, 3246, 5612) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13852) [M::mem_pestat] mean and std.dev: (3766.48, 2701.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17972) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1367, 3208, 5619) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14123) [M::mem_pestat] mean and std.dev: (3732.26, 2722.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18375) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 352.374 CPU sec, 22.459 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8876, 218168, 7771, 8696) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1260, 3041, 5635) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14385) [M::mem_pestat] mean and std.dev: (3642.01, 2753.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18760) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (228, 304, 396) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732) [M::mem_pestat] mean and std.dev: (306.38, 120.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 900) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1512, 3318, 5746) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14214) [M::mem_pestat] mean and std.dev: (3818.16, 2718.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18448) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1330, 3064, 5535) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13945) [M::mem_pestat] mean and std.dev: (3647.07, 2689.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18150) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 342.694 CPU sec, 21.848 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8722, 218785, 7825, 8450) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1266, 2935, 5430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13758) [M::mem_pestat] mean and std.dev: (3552.97, 2707.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17922) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (228, 304, 396) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732) [M::mem_pestat] mean and std.dev: (306.78, 119.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 900) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1506, 3324, 5772) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14304) [M::mem_pestat] mean and std.dev: (3825.45, 2711.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18570) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1374, 3139, 5695) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14337) [M::mem_pestat] mean and std.dev: (3717.00, 2729.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18658) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 364.894 CPU sec, 23.230 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8688, 218980, 7736, 8453) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1240, 3002, 5565) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14215) [M::mem_pestat] mean and std.dev: (3607.53, 2741.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18540) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (228, 305, 398) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738) [M::mem_pestat] mean and std.dev: (307.28, 120.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 908) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1484, 3299, 5841) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14555) [M::mem_pestat] mean and std.dev: (3826.37, 2737.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18912) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1372, 3058, 5452) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13612) [M::mem_pestat] mean and std.dev: (3620.24, 2647.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17692) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 348.230 CPU sec, 22.137 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8632, 220126, 7905, 8605) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1247, 2987, 5515) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14051) [M::mem_pestat] mean and std.dev: (3588.30, 2725.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18319) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (229, 304, 397) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733) [M::mem_pestat] mean and std.dev: (306.98, 120.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 901) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1510, 3319, 5777) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14311) [M::mem_pestat] mean and std.dev: (3821.69, 2714.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18578) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1354, 3157, 5639) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14209) [M::mem_pestat] mean and std.dev: (3712.45, 2716.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18494) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 345.281 CPU sec, 21.951 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8724, 217151, 7692, 8589) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1257, 3013, 5540) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14106) [M::mem_pestat] mean and std.dev: (3611.85, 2731.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18389) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (224, 301, 393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 731) [M::mem_pestat] mean and std.dev: (302.29, 121.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 900) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1465, 3203, 5575) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13795) [M::mem_pestat] mean and std.dev: (3730.27, 2689.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17905) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1383, 3106, 5643) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14163) [M::mem_pestat] mean and std.dev: (3692.23, 2704.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18423) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 388.742 CPU sec, 24.869 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8784, 221295, 7703, 8679) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1252, 3012, 5473) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13915) [M::mem_pestat] mean and std.dev: (3579.32, 2722.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18136) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (225, 301, 393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729) [M::mem_pestat] mean and std.dev: (303.07, 121.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 897) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1487, 3292, 5809) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14453) [M::mem_pestat] mean and std.dev: (3801.39, 2718.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18775) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1366, 3042, 5556) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13936) [M::mem_pestat] mean and std.dev: (3654.11, 2695.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18126) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 348.722 CPU sec, 22.272 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8818, 218484, 7804, 8544) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1249, 3064, 5600) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14302) [M::mem_pestat] mean and std.dev: (3638.25, 2756.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18653) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (227, 303, 395) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 731) [M::mem_pestat] mean and std.dev: (305.17, 120.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 899) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1505, 3222, 5612) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13826) [M::mem_pestat] mean and std.dev: (3744.11, 2664.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17933) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1370, 3140, 5649) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14207) [M::mem_pestat] mean and std.dev: (3707.24, 2705.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18486) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 362.325 CPU sec, 23.004 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8862, 214903, 7918, 8676) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1152, 2972, 5484) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14148) [M::mem_pestat] mean and std.dev: (3551.06, 2756.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18480) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (228, 304, 396) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732) [M::mem_pestat] mean and std.dev: (306.00, 120.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 900) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1531, 3380, 5845) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14473) [M::mem_pestat] mean and std.dev: (3870.10, 2725.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18787) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1363, 3102, 5591) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14047) [M::mem_pestat] mean and std.dev: (3679.99, 2711.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18275) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 379.417 CPU sec, 24.283 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8692, 218637, 7742, 8710) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1252, 3032, 5533) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14095) [M::mem_pestat] mean and std.dev: (3612.54, 2735.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18376) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (229, 305, 397) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733) [M::mem_pestat] mean and std.dev: (307.09, 120.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 901) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1523, 3369, 5810) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14384) [M::mem_pestat] mean and std.dev: (3842.38, 2718.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18671) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1369, 3175, 5696) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14350) [M::mem_pestat] mean and std.dev: (3758.96, 2752.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18677) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 354.596 CPU sec, 22.629 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8711, 216308, 7776, 8649) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1209, 2902, 5497) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14073) [M::mem_pestat] mean and std.dev: (3559.01, 2744.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18361) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (229, 305, 398) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736) [M::mem_pestat] mean and std.dev: (307.73, 120.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 905) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1525, 3306, 5883) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14599) [M::mem_pestat] mean and std.dev: (3864.96, 2744.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18957) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1325, 3051, 5549) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13997) [M::mem_pestat] mean and std.dev: (3633.90, 2686.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18221) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 364.899 CPU sec, 23.280 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8772, 218669, 7854, 8572) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1241, 3040, 5548) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14162) [M::mem_pestat] mean and std.dev: (3596.89, 2717.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18469) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (229, 306, 399) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739) [M::mem_pestat] mean and std.dev: (308.48, 121.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 909) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1474, 3304, 5831) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14545) [M::mem_pestat] mean and std.dev: (3841.24, 2726.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18902) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1341, 3113, 5633) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14217) [M::mem_pestat] mean and std.dev: (3697.07, 2731.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18509) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 341.167 CPU sec, 21.786 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9027, 217869, 7803, 8647) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1262, 3036, 5509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14003) [M::mem_pestat] mean and std.dev: (3599.75, 2729.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18250) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (230, 306, 399) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 737) [M::mem_pestat] mean and std.dev: (308.91, 121.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 906) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1461, 3264, 5743) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14307) [M::mem_pestat] mean and std.dev: (3806.85, 2748.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18589) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1355, 3120, 5579) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14027) [M::mem_pestat] mean and std.dev: (3667.25, 2691.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18251) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 350.816 CPU sec, 22.382 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8729, 216476, 7818, 8516) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1208, 2965, 5479) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14021) [M::mem_pestat] mean and std.dev: (3564.75, 2710.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18292) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (231, 307, 400) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738) [M::mem_pestat] mean and std.dev: (309.89, 120.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 907) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1544, 3328, 5794) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14294) [M::mem_pestat] mean and std.dev: (3832.14, 2697.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18544) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1353, 3106, 5660) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14274) [M::mem_pestat] mean and std.dev: (3706.73, 2742.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18581) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 362.246 CPU sec, 23.006 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8738, 217131, 7690, 8471) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1231, 2984, 5465) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13933) [M::mem_pestat] mean and std.dev: (3588.84, 2738.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18167) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (231, 308, 401) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741) [M::mem_pestat] mean and std.dev: (310.32, 121.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 911) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1515, 3300, 5773) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14289) [M::mem_pestat] mean and std.dev: (3824.16, 2719.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18547) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1351, 3091, 5598) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14092) [M::mem_pestat] mean and std.dev: (3659.27, 2690.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18339) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 350.847 CPU sec, 22.347 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9014, 215412, 7920, 8641) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1163, 2850, 5434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13976) [M::mem_pestat] mean and std.dev: (3514.77, 2739.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18247) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (231, 307, 401) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741) [M::mem_pestat] mean and std.dev: (310.22, 121.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 911) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1450, 3246, 5787) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14461) [M::mem_pestat] mean and std.dev: (3793.06, 2741.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18798) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1356, 3088, 5613) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14127) [M::mem_pestat] mean and std.dev: (3684.00, 2701.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18384) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 361.889 CPU sec, 23.092 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8843, 217058, 7603, 8548) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1302, 3169, 5653) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14355) [M::mem_pestat] mean and std.dev: (3684.97, 2745.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18706) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (231, 308, 401) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741) [M::mem_pestat] mean and std.dev: (310.59, 121.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 911) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1514, 3308, 5870) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14582) [M::mem_pestat] mean and std.dev: (3861.78, 2754.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18938) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1360, 3107, 5530) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13870) [M::mem_pestat] mean and std.dev: (3664.84, 2685.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18040) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 348.127 CPU sec, 22.115 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8787, 218292, 7758, 8506) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1285, 3047, 5542) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14056) [M::mem_pestat] mean and std.dev: (3632.01, 2721.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18313) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (231, 308, 401) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741) [M::mem_pestat] mean and std.dev: (310.41, 121.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 911) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1542, 3317, 5766) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14214) [M::mem_pestat] mean and std.dev: (3827.48, 2698.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18438) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1313, 3082, 5602) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14180) [M::mem_pestat] mean and std.dev: (3680.35, 2724.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18469) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 337.999 CPU sec, 21.532 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8854, 215223, 7856, 8567) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1207, 3041, 5507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14107) [M::mem_pestat] mean and std.dev: (3591.94, 2712.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18407) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (232, 309, 403) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745) [M::mem_pestat] mean and std.dev: (311.45, 121.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 916) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1504, 3307, 5791) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14365) [M::mem_pestat] mean and std.dev: (3836.63, 2730.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18652) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1406, 3148, 5664) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14180) [M::mem_pestat] mean and std.dev: (3717.79, 2704.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18438) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 361.283 CPU sec, 23.003 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8820, 216556, 7680, 8516) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1193, 2895, 5377) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13745) [M::mem_pestat] mean and std.dev: (3513.21, 2697.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17929) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (231, 308, 403) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747) [M::mem_pestat] mean and std.dev: (311.17, 122.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 919) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1522, 3340, 5957) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14827) [M::mem_pestat] mean and std.dev: (3893.11, 2770.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19262) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1381, 3114, 5600) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14038) [M::mem_pestat] mean and std.dev: (3708.42, 2714.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18257) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 343.756 CPU sec, 21.856 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8690, 216705, 7895, 8575) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1183, 2902, 5418) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13888) [M::mem_pestat] mean and std.dev: (3530.29, 2711.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18123) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (231, 308, 402) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744) [M::mem_pestat] mean and std.dev: (311.04, 121.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 915) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1532, 3318, 5883) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14585) [M::mem_pestat] mean and std.dev: (3855.69, 2728.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18936) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1382, 3182, 5717) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14387) [M::mem_pestat] mean and std.dev: (3751.00, 2742.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18722) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 340.967 CPU sec, 21.676 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8777, 219573, 7654, 8432) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1246, 3022, 5499) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14005) [M::mem_pestat] mean and std.dev: (3584.73, 2710.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18258) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (226, 302, 395) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733) [M::mem_pestat] mean and std.dev: (304.31, 121.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 902) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1483, 3279, 5777) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14365) [M::mem_pestat] mean and std.dev: (3818.96, 2745.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18659) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1374, 3186, 5740) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14472) [M::mem_pestat] mean and std.dev: (3752.76, 2734.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18838) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 354.766 CPU sec, 22.568 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8893, 220774, 7639, 8532) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1225, 3031, 5574) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14272) [M::mem_pestat] mean and std.dev: (3617.10, 2752.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18621) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (225, 300, 391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723) [M::mem_pestat] mean and std.dev: (301.73, 119.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 889) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1543, 3320, 5785) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14269) [M::mem_pestat] mean and std.dev: (3834.45, 2719.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18511) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1344, 3124, 5667) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14313) [M::mem_pestat] mean and std.dev: (3690.56, 2707.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18636) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 377.945 CPU sec, 24.125 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8849, 221008, 7491, 8564) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1242, 2962, 5508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14040) [M::mem_pestat] mean and std.dev: (3573.73, 2730.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18306) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (226, 301, 391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 721) [M::mem_pestat] mean and std.dev: (303.10, 119.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 886) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1539, 3321, 5775) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14247) [M::mem_pestat] mean and std.dev: (3836.75, 2692.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18483) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1391, 3163, 5749) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14465) [M::mem_pestat] mean and std.dev: (3742.20, 2737.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18823) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 402.881 CPU sec, 25.662 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8888, 215601, 7766, 8539) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1201, 2975, 5460) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13978) [M::mem_pestat] mean and std.dev: (3549.92, 2727.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18237) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (227, 302, 393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 725) [M::mem_pestat] mean and std.dev: (304.40, 118.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 891) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1524, 3325, 5807) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14373) [M::mem_pestat] mean and std.dev: (3835.69, 2718.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18656) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1298, 3093, 5560) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14084) [M::mem_pestat] mean and std.dev: (3656.64, 2716.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18346) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 431.693 CPU sec, 27.703 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8876, 215661, 7859, 8480) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1206, 2941, 5440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13908) [M::mem_pestat] mean and std.dev: (3553.03, 2722.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18142) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (228, 303, 395) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729) [M::mem_pestat] mean and std.dev: (305.34, 119.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1512, 3294, 5730) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14166) [M::mem_pestat] mean and std.dev: (3806.04, 2686.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18384) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1350, 3062, 5507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13821) [M::mem_pestat] mean and std.dev: (3652.93, 2701.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17978) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 394.786 CPU sec, 25.162 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8619, 208910, 7606, 8452) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1181, 2951, 5494) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14120) [M::mem_pestat] mean and std.dev: (3570.19, 2734.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18433) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (228, 303, 394) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726) [M::mem_pestat] mean and std.dev: (305.35, 119.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 892) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1462, 3288, 5705) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14191) [M::mem_pestat] mean and std.dev: (3791.62, 2715.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18434) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1335, 3026, 5514) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13872) [M::mem_pestat] mean and std.dev: (3633.19, 2698.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18051) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 461.593 CPU sec, 29.869 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8656, 222200, 7647, 8336) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1207, 2969, 5494) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14068) [M::mem_pestat] mean and std.dev: (3567.65, 2726.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18355) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (228, 303, 395) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729) [M::mem_pestat] mean and std.dev: (306.44, 119.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1520, 3275, 5691) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14033) [M::mem_pestat] mean and std.dev: (3785.34, 2700.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18204) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1320, 3064, 5637) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14271) [M::mem_pestat] mean and std.dev: (3645.94, 2703.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18588) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 424.292 CPU sec, 27.024 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9159, 211936, 8076, 8711) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1216, 3008, 5507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14089) [M::mem_pestat] mean and std.dev: (3602.13, 2764.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18380) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (230, 305, 397) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 731) [M::mem_pestat] mean and std.dev: (307.69, 119.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 898) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1499, 3301, 5796) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14390) [M::mem_pestat] mean and std.dev: (3831.32, 2736.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18687) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1369, 3090, 5667) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14263) [M::mem_pestat] mean and std.dev: (3713.30, 2745.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18561) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 464.925 CPU sec, 29.723 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9149, 209527, 7924, 8671) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1158, 2853, 5431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13977) [M::mem_pestat] mean and std.dev: (3506.03, 2725.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18250) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (230, 306, 398) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 734) [M::mem_pestat] mean and std.dev: (308.28, 119.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 902) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1453, 3228, 5733) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14293) [M::mem_pestat] mean and std.dev: (3776.18, 2716.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18573) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1411, 3130, 5539) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13795) [M::mem_pestat] mean and std.dev: (3677.31, 2661.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17923) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 443.373 CPU sec, 28.683 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9161, 215469, 7829, 8696) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1226, 3072, 5579) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14285) [M::mem_pestat] mean and std.dev: (3629.05, 2756.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18638) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (230, 306, 399) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 737) [M::mem_pestat] mean and std.dev: (308.72, 120.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 906) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1492, 3292, 5823) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14485) [M::mem_pestat] mean and std.dev: (3825.86, 2728.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18816) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1365, 3084, 5530) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13860) [M::mem_pestat] mean and std.dev: (3659.99, 2674.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18025) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 435.926 CPU sec, 27.777 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8834, 217979, 7729, 8692) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1260, 3029, 5573) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14199) [M::mem_pestat] mean and std.dev: (3625.07, 2741.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18512) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (230, 306, 398) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 734) [M::mem_pestat] mean and std.dev: (308.44, 119.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 902) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1516, 3313, 5853) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14527) [M::mem_pestat] mean and std.dev: (3857.21, 2756.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18864) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1384, 3106, 5575) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13957) [M::mem_pestat] mean and std.dev: (3684.30, 2704.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18148) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 426.120 CPU sec, 27.088 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8757, 219077, 7774, 8643) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1226, 2928, 5521) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14111) [M::mem_pestat] mean and std.dev: (3563.67, 2721.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18406) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (230, 306, 398) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 734) [M::mem_pestat] mean and std.dev: (308.51, 120.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 902) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1470, 3232, 5642) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13986) [M::mem_pestat] mean and std.dev: (3769.41, 2699.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18158) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1404, 3047, 5580) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13932) [M::mem_pestat] mean and std.dev: (3659.56, 2706.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18108) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 400.536 CPU sec, 25.475 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8989, 218431, 7745, 8673) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1264, 3073, 5588) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14236) [M::mem_pestat] mean and std.dev: (3628.52, 2732.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18560) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (230, 306, 398) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 734) [M::mem_pestat] mean and std.dev: (308.80, 120.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 902) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1530, 3349, 5878) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14574) [M::mem_pestat] mean and std.dev: (3857.42, 2735.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18922) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1363, 3076, 5552) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13930) [M::mem_pestat] mean and std.dev: (3673.43, 2710.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18119) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 408.414 CPU sec, 25.942 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8901, 218240, 7851, 8373) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1275, 3031, 5570) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14160) [M::mem_pestat] mean and std.dev: (3609.63, 2714.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18455) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (230, 306, 399) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 737) [M::mem_pestat] mean and std.dev: (309.39, 120.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 906) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1572, 3338, 5895) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14541) [M::mem_pestat] mean and std.dev: (3893.42, 2738.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18864) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1397, 3206, 5758) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14480) [M::mem_pestat] mean and std.dev: (3743.33, 2699.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18841) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 414.916 CPU sec, 26.362 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8691, 219279, 7583, 8544) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1231, 2926, 5527) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14119) [M::mem_pestat] mean and std.dev: (3566.11, 2723.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18415) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (231, 307, 398) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732) [M::mem_pestat] mean and std.dev: (309.25, 119.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 899) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1448, 3191, 5654) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14066) [M::mem_pestat] mean and std.dev: (3730.15, 2697.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18272) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1392, 3118, 5629) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14103) [M::mem_pestat] mean and std.dev: (3715.79, 2713.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18340) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 418.596 CPU sec, 26.568 real sec [M::process] read 1261462 sequences (189219300 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8849, 220672, 7708, 8559) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1232, 3005, 5577) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14267) [M::mem_pestat] mean and std.dev: (3602.82, 2714.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18612) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (230, 307, 399) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 737) [M::mem_pestat] mean and std.dev: (309.25, 120.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 906) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1530, 3325, 5759) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14217) [M::mem_pestat] mean and std.dev: (3817.86, 2699.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18446) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1319, 3031, 5565) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14057) [M::mem_pestat] mean and std.dev: (3649.16, 2709.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18303) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 377.603 CPU sec, 24.098 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8241, 210193, 7362, 8018) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1212, 2939, 5509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14103) [M::mem_pestat] mean and std.dev: (3575.37, 2739.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18400) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (230, 306, 397) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 731) [M::mem_pestat] mean and std.dev: (308.26, 119.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 898) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1508, 3311, 5720) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14144) [M::mem_pestat] mean and std.dev: (3797.03, 2692.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18356) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1393, 3048, 5604) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14026) [M::mem_pestat] mean and std.dev: (3662.28, 2676.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18237) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1261462 reads in 371.950 CPU sec, 23.578 real sec [main] Version: 0.7.17-r1188 [main] CMD: bwa mem -SP5M -t 20 /oak/stanford/groups/akundaje/marinovg/genomes/Ciliates/Tetrahymena_thermophila_SB210-E-JCVI_TeTherMIC_3/bwa-indexes/Tetrahymena_thermophila_SB210-E-JCVI_TeTherMIC_3.fa /oak/stanford/groups/akundaje/marinovg/papers/2022_Tetrahymena-Hi-C/2022-05-24-figures/juicer-Cell_24_hpm_Hi-C_rep1-GSM4161197-MIC/splits/Reads_R1.fastq.gz /oak/stanford/groups/akundaje/marinovg/papers/2022_Tetrahymena-Hi-C/2022-05-24-figures/juicer-Cell_24_hpm_Hi-C_rep1-GSM4161197-MIC/splits/Reads_R2.fastq.gz [main] Real time: 1629.194 sec; CPU: 24950.785 sec Picked up _JAVA_OPTIONS: -Xmx16384m Picked up _JAVA_OPTIONS: -Xmx16384m Picked up _JAVA_OPTIONS: -Xmx16384m Picked up _JAVA_OPTIONS: -Xmx16384m