[M::bwa_idx_load_from_disk] read 0 ALT contigs [M::process] read 5555556 sequences (200000016 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (63375, 1229506, 121294, 29436) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 196, 1382) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4004) [M::mem_pestat] mean and std.dev: (557.51, 883.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5315) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (156, 193, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (197.15, 64.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 492) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (250, 408, 741) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1723) [M::mem_pestat] mean and std.dev: (443.87, 303.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2214) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (562, 1690, 4449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12223) [M::mem_pestat] mean and std.dev: (2796.85, 2739.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16110) [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 154.395 CPU sec, 20.799 real sec [M::process] read 5555556 sequences (200000016 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (64457, 1231715, 121107, 29234) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 188, 1320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3818) [M::mem_pestat] mean and std.dev: (521.17, 822.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5067) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (156, 194, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (197.27, 64.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 492) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (252, 412, 743) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1725) [M::mem_pestat] mean and std.dev: (446.97, 306.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2216) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (557, 1670, 4458) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12260) [M::mem_pestat] mean and std.dev: (2789.41, 2742.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16161) [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 151.542 CPU sec, 17.875 real sec [M::process] read 5555556 sequences (200000016 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (59975, 1231626, 121000, 29334) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 217, 1548) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4494) [M::mem_pestat] mean and std.dev: (645.19, 1010.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5967) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (156, 194, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (197.41, 64.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 492) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (251, 410, 742) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1724) [M::mem_pestat] mean and std.dev: (445.30, 304.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2215) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (560, 1669, 4452) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12236) [M::mem_pestat] mean and std.dev: (2794.37, 2748.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16128) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 132.263 CPU sec, 9.470 real sec [M::process] read 5555556 sequences (200000016 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (63383, 1226230, 121294, 28874) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 200, 1397) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4045) [M::mem_pestat] mean and std.dev: (560.54, 882.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5369) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (157, 194, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409) [M::mem_pestat] mean and std.dev: (198.19, 64.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 493) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (250, 410, 738) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1714) [M::mem_pestat] mean and std.dev: (445.93, 304.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2202) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (560, 1674, 4386) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12038) [M::mem_pestat] mean and std.dev: (2766.11, 2708.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15864) [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 146.197 CPU sec, 16.334 real sec [M::process] read 5555556 sequences (200000016 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (64208, 1226957, 121219, 29223) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 194, 1358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3932) [M::mem_pestat] mean and std.dev: (543.03, 858.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5219) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (156, 194, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (197.46, 64.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 492) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (250, 410, 747) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1741) [M::mem_pestat] mean and std.dev: (447.66, 307.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2238) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (551, 1639, 4382) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12044) [M::mem_pestat] mean and std.dev: (2758.89, 2725.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15875) [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 133.824 CPU sec, 9.004 real sec [M::process] read 5555556 sequences (200000016 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (63889, 1224535, 120944, 29118) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 187, 1344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3892) [M::mem_pestat] mean and std.dev: (530.29, 846.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5166) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (156, 194, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (197.57, 63.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 492) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (252, 411, 741) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1719) [M::mem_pestat] mean and std.dev: (445.93, 303.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2208) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (552, 1656, 4404) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12108) [M::mem_pestat] mean and std.dev: (2757.30, 2708.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15960) [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 135.879 CPU sec, 9.525 real sec [M::process] read 5573386 sequences (200000014 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (64850, 1228177, 120932, 29124) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 180, 1269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3671) [M::mem_pestat] mean and std.dev: (492.61, 787.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4872) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (156, 193, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (197.00, 64.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 492) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (251, 410, 738) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1712) [M::mem_pestat] mean and std.dev: (445.53, 303.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2199) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (549, 1642, 4384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12054) [M::mem_pestat] mean and std.dev: (2766.24, 2725.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15889) [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5573386 reads in 132.212 CPU sec, 9.357 real sec [M::process] read 5714286 sequences (200000010 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55873, 1254871, 125464, 29962) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 287, 1851) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5385) [M::mem_pestat] mean and std.dev: (834.17, 1255.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7152) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (157, 195, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 412) [M::mem_pestat] mean and std.dev: (198.68, 64.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 497) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (250, 408, 738) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1714) [M::mem_pestat] mean and std.dev: (444.25, 304.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2202) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (558, 1646, 4412) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12120) [M::mem_pestat] mean and std.dev: (2766.29, 2726.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15974) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5714286 reads in 151.779 CPU sec, 17.590 real sec [M::process] read 5714286 sequences (200000010 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (57102, 1256101, 124559, 29748) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 266, 1833) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5337) [M::mem_pestat] mean and std.dev: (818.54, 1243.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7089) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (156, 194, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411) [M::mem_pestat] mean and std.dev: (197.93, 64.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 496) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (251, 411, 744) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1730) [M::mem_pestat] mean and std.dev: (446.43, 305.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2223) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (552, 1671, 4460) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12276) [M::mem_pestat] mean and std.dev: (2781.87, 2736.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16184) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5714286 reads in 135.394 CPU sec, 9.242 real sec [M::process] read 5714286 sequences (200000010 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (59824, 1249271, 124996, 29656) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 244, 1684) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4894) [M::mem_pestat] mean and std.dev: (727.99, 1123.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6499) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (157, 195, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 415) [M::mem_pestat] mean and std.dev: (199.10, 65.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (250, 409, 741) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1723) [M::mem_pestat] mean and std.dev: (445.09, 305.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2214) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (556, 1652, 4400) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12088) [M::mem_pestat] mean and std.dev: (2772.83, 2732.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15932) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5714286 reads in 141.659 CPU sec, 11.594 real sec [M::process] read 5714286 sequences (200000010 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (57980, 1251022, 124363, 29743) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 259, 1786) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5198) [M::mem_pestat] mean and std.dev: (790.64, 1209.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6904) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (157, 194, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409) [M::mem_pestat] mean and std.dev: (198.25, 64.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 493) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (250, 410, 741) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1723) [M::mem_pestat] mean and std.dev: (445.91, 306.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2214) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (552, 1651, 4437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12207) [M::mem_pestat] mean and std.dev: (2786.64, 2746.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16092) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5714286 reads in 135.246 CPU sec, 9.471 real sec [M::process] read 5714286 sequences (200000010 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55950, 1251131, 125475, 30064) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 288, 1878) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5462) [M::mem_pestat] mean and std.dev: (851.25, 1275.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7254) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (158, 195, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 413) [M::mem_pestat] mean and std.dev: (199.41, 64.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 498) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (253, 413, 745) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1729) [M::mem_pestat] mean and std.dev: (448.52, 306.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2221) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (557, 1658, 4402) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12092) [M::mem_pestat] mean and std.dev: (2768.74, 2717.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15937) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5714286 reads in 136.524 CPU sec, 10.499 real sec [M::process] read 5714286 sequences (200000010 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (56025, 1253331, 124423, 29846) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 270, 1854) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5396) [M::mem_pestat] mean and std.dev: (833.28, 1262.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7167) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (157, 195, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 412) [M::mem_pestat] mean and std.dev: (198.69, 64.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 497) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (251, 410, 748) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1742) [M::mem_pestat] mean and std.dev: (447.39, 307.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2239) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (568, 1688, 4412) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12100) [M::mem_pestat] mean and std.dev: (2775.42, 2708.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15944) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5714286 reads in 138.918 CPU sec, 9.763 real sec [M::process] read 5714286 sequences (200000010 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55542, 1246995, 125782, 29756) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 283, 1869) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5435) [M::mem_pestat] mean and std.dev: (842.25, 1269.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7218) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (158, 196, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 413) [M::mem_pestat] mean and std.dev: (199.66, 64.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 498) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (251, 409, 737) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1709) [M::mem_pestat] mean and std.dev: (444.92, 302.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2195) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (554, 1669, 4378) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12026) [M::mem_pestat] mean and std.dev: (2763.00, 2712.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15850) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5714286 reads in 137.910 CPU sec, 10.351 real sec [M::process] read 5700226 sequences (200000026 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (59217, 1247671, 123591, 29740) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 239, 1721) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5009) [M::mem_pestat] mean and std.dev: (742.29, 1149.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6653) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (156, 194, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411) [M::mem_pestat] mean and std.dev: (198.06, 64.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 496) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (251, 411, 739) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1715) [M::mem_pestat] mean and std.dev: (445.57, 303.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2203) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (562, 1657, 4385) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12031) [M::mem_pestat] mean and std.dev: (2770.51, 2722.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15854) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5700226 reads in 146.916 CPU sec, 14.896 real sec [M::process] read 5555556 sequences (200000016 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (60627, 1229505, 121274, 29203) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 219, 1526) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4428) [M::mem_pestat] mean and std.dev: (636.93, 989.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5879) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (156, 194, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (197.51, 64.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 492) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (251, 410, 742) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1724) [M::mem_pestat] mean and std.dev: (444.32, 304.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2215) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (564, 1644, 4412) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12108) [M::mem_pestat] mean and std.dev: (2773.43, 2736.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15956) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 130.682 CPU sec, 9.399 real sec [M::process] read 5555556 sequences (200000016 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (64302, 1232797, 119822, 29010) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 185, 1331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3857) [M::mem_pestat] mean and std.dev: (523.69, 837.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5120) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (155, 192, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 404) [M::mem_pestat] mean and std.dev: (195.86, 63.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 487) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (251, 413, 749) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1745) [M::mem_pestat] mean and std.dev: (449.41, 309.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2243) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (556, 1637, 4477) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12319) [M::mem_pestat] mean and std.dev: (2788.38, 2748.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16240) [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 143.117 CPU sec, 13.278 real sec [M::process] read 5555556 sequences (200000016 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (64196, 1225572, 121123, 29160) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 189, 1284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3712) [M::mem_pestat] mean and std.dev: (505.98, 798.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4926) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (156, 194, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (197.29, 64.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 492) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (252, 412, 746) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1734) [M::mem_pestat] mean and std.dev: (447.49, 306.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2228) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (559, 1658, 4380) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12022) [M::mem_pestat] mean and std.dev: (2758.95, 2714.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15843) [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 135.867 CPU sec, 10.047 real sec [M::process] read 5555556 sequences (200000016 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (64956, 1225420, 120583, 28978) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 185, 1328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3844) [M::mem_pestat] mean and std.dev: (525.02, 836.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5102) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (156, 193, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405) [M::mem_pestat] mean and std.dev: (196.69, 63.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 488) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (252, 413, 749) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1743) [M::mem_pestat] mean and std.dev: (448.90, 307.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2240) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (555, 1643, 4404) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12102) [M::mem_pestat] mean and std.dev: (2760.86, 2712.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15951) [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 147.706 CPU sec, 17.372 real sec [M::process] read 5555556 sequences (200000016 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (66044, 1226787, 120175, 29246) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 175, 1221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3529) [M::mem_pestat] mean and std.dev: (471.28, 753.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4683) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (155, 193, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 407) [M::mem_pestat] mean and std.dev: (196.20, 63.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 491) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (252, 412, 746) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1734) [M::mem_pestat] mean and std.dev: (447.89, 306.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2228) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (553, 1654, 4406) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12112) [M::mem_pestat] mean and std.dev: (2775.45, 2739.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15965) [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 133.742 CPU sec, 9.440 real sec [M::process] read 5555556 sequences (200000016 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (62681, 1226016, 121125, 29120) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 196, 1378) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3990) [M::mem_pestat] mean and std.dev: (551.19, 871.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5296) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (156, 193, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (196.99, 64.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 492) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (250, 413, 747) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1741) [M::mem_pestat] mean and std.dev: (447.47, 307.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2238) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (556, 1668, 4417) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12139) [M::mem_pestat] mean and std.dev: (2778.63, 2722.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16000) [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 148.878 CPU sec, 16.207 real sec [M::process] read 5555556 sequences (200000016 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (63196, 1230509, 120999, 29072) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 196, 1382) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4002) [M::mem_pestat] mean and std.dev: (554.25, 876.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5312) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (156, 193, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (197.23, 63.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 492) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (251, 412, 747) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1739) [M::mem_pestat] mean and std.dev: (448.26, 308.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2235) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (550, 1678, 4472) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12316) [M::mem_pestat] mean and std.dev: (2785.16, 2732.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16238) [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 133.295 CPU sec, 9.677 real sec [M::process] read 5555556 sequences (200000016 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (65278, 1227044, 121749, 29192) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 188, 1275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3681) [M::mem_pestat] mean and std.dev: (500.34, 790.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4884) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (157, 194, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409) [M::mem_pestat] mean and std.dev: (198.28, 64.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 493) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (251, 409, 739) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1715) [M::mem_pestat] mean and std.dev: (445.54, 303.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2203) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (571, 1683, 4386) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12016) [M::mem_pestat] mean and std.dev: (2786.21, 2727.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15831) [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 145.999 CPU sec, 14.879 real sec [M::process] read 5555556 sequences (200000016 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (63228, 1230892, 120866, 29053) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 194, 1392) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4034) [M::mem_pestat] mean and std.dev: (562.80, 888.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5355) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (155, 193, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 407) [M::mem_pestat] mean and std.dev: (196.45, 63.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 491) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (251, 409, 738) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1712) [M::mem_pestat] mean and std.dev: (443.92, 302.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2199) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (563, 1658, 4420) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12134) [M::mem_pestat] mean and std.dev: (2779.99, 2729.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15991) [M::mem_pestat] skip orientation RR [M::process] read 5555556 sequences (200000016 bp)... [M::mem_process_seqs] Processed 5555556 reads in 137.049 CPU sec, 10.181 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (63816, 1226759, 120904, 29286) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 194, 1381) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3999) [M::mem_pestat] mean and std.dev: (550.67, 874.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5308) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (156, 194, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (197.54, 63.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 492) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (251, 410, 743) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1727) [M::mem_pestat] mean and std.dev: (445.39, 304.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2219) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (558, 1665, 4429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12171) [M::mem_pestat] mean and std.dev: (2772.68, 2726.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16042) [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 5555556 reads in 147.844 CPU sec, 17.439 real sec [M::process] read 28884 sequences (1039824 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (301, 6407, 623, 130) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 286, 2074) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6022) [M::mem_pestat] mean and std.dev: (1033.88, 1536.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7996) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (158, 196, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416) [M::mem_pestat] mean and std.dev: (200.26, 64.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 502) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (250, 403, 765) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1795) [M::mem_pestat] mean and std.dev: (475.70, 349.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2310) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (677, 1892, 4953) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13505) [M::mem_pestat] mean and std.dev: (2986.98, 2650.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17781) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 28884 reads in 1.219 CPU sec, 0.100 real sec [main] Version: 0.7.17-r1188 [main] CMD: bwa mem -SP5M -t 20 /oak/stanford/groups/akundaje/marinovg/genomes/Archaea/Euryarchaeota/Haloferax_volcanii_DS2_uid46845/bwa-indexes/all.fa /oak/stanford/groups/akundaje/marinovg/papers/2021_Haloferax-chromatin/2021-09-14-Hi-C-rerun/juicer-CC375_H._volcanii_dOriC1-3_dOri-pHV4_HiC/splits/reads_R1.fastq.gz /oak/stanford/groups/akundaje/marinovg/papers/2021_Haloferax-chromatin/2021-09-14-Hi-C-rerun/juicer-CC375_H._volcanii_dOriC1-3_dOri-pHV4_HiC/splits/reads_R2.fastq.gz [main] Real time: 683.065 sec; CPU: 3729.029 sec slurmstepd: error: *** JOB 33559990 ON sh02-02n29 CANCELLED AT 2021-09-20T11:21:45 ***