Genome-wide predicted enhancer activity in TF occupied cEnhancers. 
Functional assay testing of Enhancer regulatory activity was performed in C2C12 cells (Muscle); K562 (Myelogenous) and G1E-ER4  (Erythroid) for TF selected cEnhancers. Elements were subselected for stringent IDR (0.05) TF occupancy and presence of H3K27Ac+DHS. The number of elements scored for both a negative control set (NC) and experimental group (E) are represented above each plot individually. The proportion of elements active in the Experimental group is then used to extrapolate the predicted activity in similarly marked regions genome-wide. The negative control group was selected from genomic regions that show no TF occupancy or DHS; and in the cases of C2C12 and Erythroid required the presence of the TF occupancy motif. 

Genome-wide predicted enhancer activity in TF agnostic cEnhancers. 
Functional assay testing of Enhancer regulatory activity was performed in K562 cells (Myelogenous); HepG2 (Liver) and G1E-ER4  (Erythroid) for TF agnostic cEnhancers. Only elements that showed the presence of stringent (0.05) IDR H3K27Ac+DHS peak calls were selected. The proportion of elements active in the Experimental group is then used to extrapolate the predicted activity in similarly marked regions genome-wide. The negative control group was selected from genomic regions that show no DHS or H3K27Ac; for the Erythroid GATA1 conserved motif control group we required the presence of conserved TF occupancy motif with no biochemical marks.