silkworm_tRNA.gff is the annotation result for silkworm tRNA. All on one line, for example here (with descriptions): ------ nscaf1071 tRNAscan tRNA 1242603 1242675 76.04 + . GenePrediction BGIBMTRNAA000001 aa:Val codon:GTA structure:>>>>>>>..>>>..........<<<.>>>>>.......<<<<<.....>>>>>.......<<<<<<<<<<<<. ------ Where: nscaf1071: is the id of the genome sequence. tRNAscan: is the method tRNA: is the kind of gene. 1242603: is the start of the tRNA gene. 1242675: is the end of the tRNA gene. 76.04: is the score of prediction. +: is the strand of gene. GIBMTRNAA000001: is the id of the tRNA gene. aa:Val: is the kind of tRNA. codon:GTA: is the codon of tRNA gene. structure:>>>>>>>..>>>..........<<<.>>>>>.......<<<<<.....>>>>>.......<<<<<<<<<<<<.: is the structure of tRNA gene.