PEAKXUS RESULTS

SUMMARY

Total number of peaks (no FDR-cutoff)

=

272868.0

Total number of reads

=

21515754.0

Total number of UMIs

=

21515754.0

Reads/UMIs

=

1.0

Quality control results from FastQC for the input library.

The best MEME-motif:

Motif 1Motif 1 rc

Full MEME-results

PEAK SIZE STATISTICS

Peak score histogram
Peak size histogram
Histogram of peak scores for all reported peaks.
Histogram of unique read 5'-end counts within peak borders (Reads pointing towards peak summit minus reads pointing away from peak summit). If UMIs were not used in the analysis, raw read counts are reported.

HISTOGRAM OF UMI-COUNTS

+ strand UMI-count histogram- strand UMI-count histogram
Histogram of UMI-counts per position on the sense strand.
Histogram of UMI-counts per position on the antisense strand.

BREAKDOWN OF TOP 1000 PEAKS

Top 1000 peaksTop 1000 peaks
Average read 5'-end count around the top 1000 peaks. Red curve corresponds to read 5'-end counts on the sense strand, and blue on the antisense strand, respectively.
Heatmap presentation of the same peaks shown on the left. Red color marks the sense strand read 5'-end count, and blue the antisense strand, respectively.

INPUT PARAMETERS FOR PEAKXUS

B2=None
N4=1000
N6=1000
S4=1
UMIs=None
adapters1=None
b3=0.05
blacklist=None
center6=0
chromnames='/oak/stanford/groups/akundaje/marinovg/genomes/dm6/dm6.chrom.sizes'
e4='PeakXus'
fastq='Dme_wts_SD_wingdisc_ChIP-nexus_1-GSM2545322/aligned.25mers.unique.bam'
genome2=None
k4=4
l3=5
l6='PeakXus'
l_l3=60
m4=10
m5=20
m6=100
matrixhits=None
maxw7=50
minw7=4
ml4=17
ms4=-8
n3=1
n4=1
o5=1
outdir='Dme_wts_SD_wingdisc_ChIP-nexus_1-GSM2545322/peakXus/'
p3=1.0
p7=1
q2=20
s3=1
skip0=0
t2=1
title5='PeakXus'
verbosity=1
w3=5
w4=100
wg7=None)Testing that input files exist...done!

If you use PeakXus in your work, please cite:
Hartonen, T., Sahu, B., Dave, K., Kivioja, T., & Taipale, J. (2016). PeakXus: comprehensive transcription factor binding site discovery from ChIP-Nexus and ChIP-Exo experiments. Bioinformatics, 32(17), i629-i638.