FastQCFastQC Report
Mon 14 Aug 2023
stdin:025999-L478_D6_D10_sgRNA_720_CL14-UGAv3-250_trimmed

Summary

[OK]Basic Statistics

MeasureValue
Filename025999-L478_D6_D10_sgRNA_720_CL14-UGAv3-250_trimmed
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15240769
Sequences flagged as poor quality0
Sequence length9-371
%GC47

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTGTTTCGTCCTTTCCACAAGAGATCGGAAGAGCGTCGTGTAGGGAAA588280.3859910218441078Illumina Single End PCR Primer 1 (100% over 27bp)
CAAGCAGAAGACGGCATACGAGATCTGTAGCCGTGGCCGGTGTTTCGTCC301400.1977590500846775RNA PCR Primer, Index 17 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATCAACCACAGTGGCCGATGTTTCGCAT223220.14646242587890415RNA PCR Primer, Index 23 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATAGTACAAGGTGGCCGATGTTTCGCAT210100.1378539363728956RNA PCR Primer, Index 22 (96% over 29bp)
GTGTTTCGTCCTTTCCACAAGAGATCGGAAGAGCGTCGTGTAGGGAAAGA173840.11406248595461292Illumina Single End PCR Primer 1 (100% over 29bp)
CAAGCAGAAGACGGCATACGAGATACAGCAGAGTGGCCGATGTTTCGCAT168660.11066370732342969Illumina PCR Primer Index 2 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATGTACGCAAGTGGCCGATGTTTCGGTC167800.11009943133446876RNA PCR Primer, Index 16 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)