FastQCFastQC Report
Mon 14 Aug 2023
stdin:025999-L476_D6_D10_sgRNA_738_CL10-UGAv3-37_trimmed

Summary

[OK]Basic Statistics

MeasureValue
Filename025999-L476_D6_D10_sgRNA_738_CL10-UGAv3-37_trimmed
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17423958
Sequences flagged as poor quality0
Sequence length11-370
%GC46

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTTCGTCCTTTCCACAAGAGATCGGAAGAGCGTCGTGTAGGGAAAGA338430.19423256185534885Illumina Single End PCR Primer 1 (100% over 29bp)
CAAGCAGAAGACGGCATACGAGATCGCATACAGTGGCCGATGTTTCGCAT253420.1454434176207266RNA PCR Primer, Index 33 (96% over 29bp)
CGGTGTTTCGTCCTTTCCACAAGAGATCGGAAGAGCGTCGTGTAGGGAAA251820.14452514176170533Illumina Single End PCR Primer 1 (100% over 27bp)
CAAGCAGAAGACGGCATACGAGATAGTGGTCAGTGGCCGATGTTTCGCAT208520.11967430132694305Illumina PCR Primer Index 6 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATACACGACCGTGGCCGATGTTTCGCAT187140.10740384016077174RNA PCR Primer, Index 23 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATATCCTGTAGTGGCCGATGTTTCGCAT185350.10637651904349174RNA PCR Primer, Index 31 (96% over 30bp)
CAAGCAGAAGACGGCATACGAGATCAACCACAGTGGCCGATGTTTCGCAT180870.10380534663823225RNA PCR Primer, Index 23 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATCTGAGCCAGTGGCCGATGTTTCGCAT180580.10363890913878465Illumina PCR Primer Index 9 (96% over 30bp)
CAAGCAGAAGACGGCATACGAGATACCACTGTGTGGCCGATGTTTCGCAT179630.10309368284749079TruSeq Adapter, Index 25 (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)