FastQCFastQC Report
Mon 14 Aug 2023
stdin:025999-L475_D6_D10_sgRNA_737_CL09-UGAv3-172_trimmed

Summary

[OK]Basic Statistics

MeasureValue
Filename025999-L475_D6_D10_sgRNA_737_CL09-UGAv3-172_trimmed
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20454525
Sequences flagged as poor quality0
Sequence length9-383
%GC46

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTGTTTCGTCCTTTCCACAAGAGATCGGAAGAGCGTCGTGTAGGGAAA344630.16848594626372404Illumina Single End PCR Primer 1 (100% over 27bp)
CAAGCAGAAGACGGCATACGAGATAGATCGCAGTGGCCGATGTTTCGCAT242080.1183503405725628Illumina PCR Primer Index 2 (96% over 30bp)
CAAGCAGAAGACGGCATACGAGATACCACTGTGTGGCCGATGTTTCGCAT220510.10780499669388557TruSeq Adapter, Index 25 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATAGCAGGAAGTGGCCGATGTTTCGCAT216620.10590321701432813TruSeq Adapter, Index 25 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATGCGAGTAAGTGGCCGATGTTTCGCAT211360.10333165888721445RNA PCR Primer, Index 24 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATACACAGAAGTGGCCGATGTTTCGCAT210770.10304321415432525RNA PCR Primer, Index 23 (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)