FastQCFastQC Report
Mon 14 Aug 2023
stdin:025999-L474_D6_D10_sgRNA_727_CL03-UGAv3-200_trimmed

Summary

[OK]Basic Statistics

MeasureValue
Filename025999-L474_D6_D10_sgRNA_727_CL03-UGAv3-200_trimmed
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23113338
Sequences flagged as poor quality0
Sequence length9-366
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATTGAAGCCAGTGACTGGAGTTCAGACG4655872.0143650389225476Illumina PCR Primer Index 8 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATTGGTCCGTGTGACTGGAGTTCAGACG344440.14902217931481815Illumina PCR Primer Index 4 (96% over 33bp)
GTGTTTCGTCCTTTCCACAAGAGATCGGAAGAGCGTCGTGTAGGGAAAGA247580.10711564032854103Illumina Single End PCR Primer 1 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)