FastQCFastQC Report
Mon 14 Aug 2023
stdin:025999-L472_D6_D10_sgRNA_724_CL01-UGAv3-67_trimmed

Summary

[OK]Basic Statistics

MeasureValue
Filename025999-L472_D6_D10_sgRNA_724_CL01-UGAv3-67_trimmed
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35097845
Sequences flagged as poor quality0
Sequence length10-371
%GC46

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTTCGTCCTTTCCACAAGAGATCGGAAGAGCGTCGTGTAGGGAAAGA573010.16326073580870848Illumina Single End PCR Primer 1 (100% over 29bp)
CAAGCAGAAGACGGCATACGAGATGCTCGGTAGTGGCCGATGTTTCGCAT540000.15385559996632273Illumina PCR Primer Index 7 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATACACAGAAGTGGCCGATGTTTCGCAT499830.1424104528354946RNA PCR Primer, Index 23 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATTGAAGCCAGTGACTGGAGTTCAGACG498820.14212268588000204Illumina PCR Primer Index 8 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATACACGACCGTGGCCGATGTTTCGCAT387990.11054524857580286RNA PCR Primer, Index 23 (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)