stdin:025999-L471_D6_D10_RNA_CL14-UGAv3-162

Basic Statistics

Measure	Value
Filename	025999-L471_D6_D10_RNA_CL14-UGAv3-162_trimmed
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	343615645
Sequences flagged as poor quality	0
Sequence length	8-497
%GC	48

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CAAGCAGAAGACGGCATACGAGATCATACCAAGTGGCCGATGTTTCGCAT	760865	0.22142909121614646	RNA PCR Primer, Index 22 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATCACTTCGAGTGGCCGATGTTTCGCAT	747790	0.21762396761649197	Illumina PCR Primer Index 5 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATAGTCACTAGTGGCCGATGTTTCGCAT	730625	0.21262856061166832	RNA PCR Primer, Index 37 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATAGCAGGAAGTGGCCGATGTTTCGCAT	730048	0.21246064043445984	TruSeq Adapter, Index 25 (96% over 29bp)
CCTTCATCAGGGTTTGCTGAAGATGGCGGTATATAGGCTGAGCAAGAGGT	723286	0.21049274400762513	No Hit
CAAGCAGAAGACGGCATACGAGATAGTACAAGGTGGCCGATGTTTCGCAT	711138	0.2069573985782865	RNA PCR Primer, Index 22 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATACACAGAAGTGGCCGATGTTTCGCAT	705677	0.20536812286297385	RNA PCR Primer, Index 23 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATACAGCAGAGTGGCCGATGTTTCGCAT	701752	0.20422585822598388	Illumina PCR Primer Index 2 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATACCTCCAAGTGGCCGATGTTTCGCAT	697509	0.2029910483266849	Illumina PCR Primer Index 2 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATACGCTCGAGTGGCCGATGTTTCGCAT	694702	0.20217414722196367	Illumina PCR Primer Index 10 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATCCAGTTCAGTGGCCGATGTTTCGCAT	693073	0.20170007101975815	RNA PCR Primer, Index 23 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATCTCAATGAGTGGCCGATGTTTCGCAT	674057	0.19616598074281513	RNA PCR Primer, Index 17 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATAACGTGATGTGGCCGATGTTTCGCAT	672405	0.19568521101534825	RNA PCR Primer, Index 31 (96% over 30bp)
CAAGCAGAAGACGGCATACGAGATCGCATACAGTGGCCGATGTTTCGCAT	662248	0.19272929205537193	RNA PCR Primer, Index 33 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATAGTGGTCAGTGGCCGATGTTTCGCAT	662197	0.192714449890662	Illumina PCR Primer Index 6 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATACAAGCTAGTGGCCGATGTTTCGCAT	660247	0.19214695535763512	Illumina PCR Primer Index 8 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATAGATCGCAGTGGCCGATGTTTCGCAT	657787	0.19143103917750895	Illumina PCR Primer Index 2 (96% over 30bp)
CAAGCAGAAGACGGCATACGAGATAAGGTACAGTGGCCGATGTTTCGCAT	653457	0.19017091029135183	Illumina PCR Primer Index 10 (96% over 30bp)
CAAGCAGAAGACGGCATACGAGATCAGCGTTAGTGGCCGATGTTTCGCAT	650944	0.18943956990084082	Illumina PCR Primer Index 10 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATCGCTGATCGTGGCCGATGTTTCGCAT	649415	0.18899459598238025	Illumina PCR Primer Index 5 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATCCATCCTCGTGGCCGATGTTTCGCAT	633975	0.18450120337215728	Illumina PCR Primer Index 2 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATGCGAGTAAGTGGCCGATGTTTCGCAT	629242	0.18312379228250797	RNA PCR Primer, Index 24 (96% over 28bp)
GTGTAGATCTCGGTGGTCGCCGTATCATTAGCTGAGACTGCCAAGGCACACAGGG	619143	0.1801847526471037	Illumina Single End PCR Primer 1 (100% over 29bp)
CAAGCAGAAGACGGCATACGAGATACAGATTCGTGGCCGATGTTTCGCAT	617338	0.17965945642550704	Illumina PCR Primer Index 2 (96% over 27bp)
CAAGCAGAAGACGGCATACGAGATATCCTGTAGTGGCCGATGTTTCGCAT	608668	0.17713628842481838	RNA PCR Primer, Index 31 (96% over 30bp)
CAAGCAGAAGACGGCATACGAGATGCTAACGAGTGGCCGATGTTTCGCAT	607481	0.17679084431676562	RNA PCR Primer, Index 24 (96% over 31bp)
CAAGCAGAAGACGGCATACGAGATAACAACCAGTGGCCGATGTTTCGCAT	605799	0.17630134390417526	Illumina PCR Primer Index 12 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATTGGTGGTAGTGGCCGATGTTTCGCAT	597822	0.1739798547298392	RNA PCR Primer, Index 36 (96% over 30bp)
CAAGCAGAAGACGGCATACGAGATACACGACCGTGGCCGATGTTTCGCAT	593990	0.17286465521673205	RNA PCR Primer, Index 23 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATCTGAGCCAGTGGCCGATGTTTCGCAT	586407	0.17065782904035118	Illumina PCR Primer Index 9 (96% over 30bp)
CAAGCAGAAGACGGCATACGAGATCTGGCATAGTGGCCGATGTTTCGCAT	584229	0.1700239813003858	RNA PCR Primer, Index 30 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATAACCGAGAGTGGCCGATGTTTCGCAT	580620	0.16897367988002993	Illumina PCR Primer Index 10 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATTATCAGCAGTGGCCGATGTTTCGCAT	579691	0.16870331966403915	RNA PCR Primer, Index 19 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATCATCAAGTGTGGCCGATGTTTCGCAT	577180	0.16797256131920305	Illumina PCR Primer Index 7 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATCAACCACAGTGGCCGATGTTTCGCAT	576593	0.16780173091362008	RNA PCR Primer, Index 23 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATCAGATCTGGTGGCCGATGTTTCGCAT	575819	0.16757647923743402	Illumina PCR Primer Index 9 (96% over 30bp)
CAAGCAGAAGACGGCATACGAGATAAACATCGGTGGCCGATGTTTCGCAT	575136	0.1673777106394559	Illumina PCR Primer Index 10 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATACATTGGCGTGGCCGATGTTTCGCAT	566880	0.1649750260934714	Illumina PCR Primer Index 2 (96% over 31bp)
CAAGCAGAAGACGGCATACGAGATCCGAAGTAGTGGCCGATGTTTCGCAT	562799	0.16378736189383927	Illumina PCR Primer Index 9 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATTTCACGCAGTGGCCGATGTTTCGCAT	558890	0.16264975362224848	RNA PCR Primer, Index 13 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATCGACTGGAGTGGCCGATGTTTCGCAT	553074	0.16095716479964117	RNA PCR Primer, Index 33 (96% over 31bp)
CAAGCAGAAGACGGCATACGAGATCCGTGAGAGTGGCCGATGTTTCGCAT	545244	0.15867845598241023	Illumina Paired End PCR Primer 2 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATGTCTGTCAGTGGCCGATGTTTCGCAT	544464	0.15845145816919948	Illumina PCR Primer Index 3 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATACGTATCAGTGGCCGATGTTTCGCAT	538743	0.1567865165161499	Illumina PCR Primer Index 2 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATAACGCTTAGTGGCCGATGTTTCGCAT	537624	0.1564608619610437	RNA PCR Primer, Index 31 (96% over 28bp)
TTCTAAGTGTGTAGATCTCGGTGGTCGCCGTATCATTAGCTGAGACTGCCAAGGCACACAGGGGATAGGC	533679	0.15531277686730474	Illumina Single End PCR Primer 1 (96% over 33bp)
CAAGCAGAAGACGGCATACGAGATACCACTGTGTGGCCGATGTTTCGCAT	533206	0.15517512306519105	TruSeq Adapter, Index 25 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATCAAGACTAGTGGCCGATGTTTCGCAT	521218	0.15168634128984435	RNA PCR Primer, Index 21 (96% over 27bp)
CAAGCAGAAGACGGCATACGAGATTAGGATGAGTGGCCGATGTTTCGCAT	519447	0.151170939844721	RNA PCR Primer, Index 46 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATAGATGTACGTGGCCGATGTTTCGCAT	515990	0.15016487389565744	Illumina PCR Primer Index 2 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATAACTCACCGTGGCCGATGTTTCGCAT	508251	0.1479126481566344	Illumina PCR Primer Index 5 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATAGCACCTCGTGGCCGATGTTTCGCAT	506921	0.14752558778282637	TruSeq Adapter, Index 25 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATATTGAGGAGTGGCCGATGTTTCGCAT	500130	0.1455492516937056	Illumina PCR Primer Index 6 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATTCCGTCTAGTGGCCGATGTTTCGCAT	493254	0.14354817866340167	RNA PCR Primer, Index 40 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATCTAAGGTCGTGGCCGATGTTTCGCAT	487928	0.1419981910311447	Illumina PCR Primer Index 9 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATGAATCTGAGTGGCCGATGTTTCGCAT	486730	0.14164954567187998	RNA PCR Primer, Index 39 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATCAAGGAGCGTGGCCGATGTTTCGCAT	485796	0.141377730341702	RNA PCR Primer, Index 21 (96% over 27bp)
CAAGCAGAAGACGGCATACGAGATCCTCCTGAGTGGCCGATGTTTCGCAT	484525	0.14100784031530345	RNA PCR Primer, Index 23 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATGCTCGGTAGTGGCCGATGTTTCGCAT	477070	0.13883826506211613	Illumina PCR Primer Index 7 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATCTGTAGCCGTGGCCGATGTTTCGCAT	463811	0.1349795932603709	RNA PCR Primer, Index 17 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATGCCACATAGTGGCCGATGTTTCGCAT	461430	0.13428666788440322	RNA PCR Primer, Index 24 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATATGCCTAAGTGGCCGATGTTTCGCAT	460636	0.1340555957514682	RNA PCR Primer, Index 37 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATCACCTTACGTGGCCGATGTTTCGCAT	459004	0.13358064648075033	RNA PCR Primer, Index 33 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATGTCGTAGAGTGGCCGATGTTTCGCAT	457426	0.13312141244325473	RNA PCR Primer, Index 41 (96% over 31bp)
CAAGCAGAAGACGGCATACGAGATACTATGCAGTGGCCGATGTTTCGCAT	451570	0.13141718270714944	RNA PCR Primer, Index 44 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATTGGAACAAGTGGCCGATGTTTCGCAT	446830	0.13003773445763797	RNA PCR Primer, Index 27 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATGATAGACAGTGGCCGATGTTTCGCAT	445037	0.12951593051009072	RNA PCR Primer, Index 24 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATGACTAGTAGTGGCCGATGTTTCGCAT	442014	0.12863616847248036	Illumina PCR Primer Index 3 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATGTGTTCTAGTGGCCGATGTTTCGCAT	433502	0.12615898208010873	RNA PCR Primer, Index 39 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATGAGCTGAAGTGGCCGATGTTTCGCAT	414701	0.12068746171321738	Illumina PCR Primer Index 7 (96% over 30bp)
CAAGCAGAAGACGGCATACGAGATTGGCTTCAGTGGCCGATGTTTCGCAT	414143	0.12052507096992048	RNA PCR Primer, Index 15 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATAAGACGGAGTGGCCGATGTTTCGCAT	413225	0.12025791200514167	RNA PCR Primer, Index 27 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATAGAGTCAAGTGGCCGATGTTTCGCAT	411581	0.11977947046037442	RNA PCR Primer, Index 32 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATTGAAGAGAGTGGCCGATGTTTCGCAT	409770	0.11925242810175306	Illumina PCR Primer Index 8 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATGACAGTGCGTGGCCGATGTTTCGCAT	409616	0.11920761058478581	Illumina PCR Primer Index 12 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATGGTGCGAAGTGGCCGATGTTTCGCAT	409334	0.11912554214462498	Illumina PCR Primer Index 1 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATTCTTCACAGTGGCCGATGTTTCGCAT	403179	0.11733429658012223	RNA PCR Primer, Index 36 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATAATGTTGCGTGGCCGATGTTTCGCAT	400954	0.11668677076679672	Illumina PCR Primer Index 6 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATGGAGAACAGTGGCCGATGTTTCGCAT	398774	0.11605234098115644	Illumina PCR Primer Index 11 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATCCTAATCCGTGGCCGATGTTTCGCAT	391466	0.11392554608507421	RNA PCR Primer, Index 22 (96% over 28bp)
TTCTAAGTGTGTAGATCTCGGTGGTCGCCGTATCATTAGCTGAGACTGCCAAGGCACACAGGGGATAG	388606	0.11309322076996815	Illumina Single End PCR Primer 1 (96% over 33bp)
GTGTAGATCTCGGTGGTCGCCGTATCATTAGCTGAGACTGCCAAGGCACACAGGGGATAGGC	385614	0.11222248044031871	Illumina Single End PCR Primer 1 (100% over 29bp)
CAAGCAGAAGACGGCATACGAGATAAGAGATCGTGGCCGATGTTTCGCAT	383669	0.11165644102147909	RNA PCR Primer, Index 27 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATCGACACACGTGGCCGATGTTTCGCAT	383610	0.11163927067406956	RNA PCR Primer, Index 21 (96% over 30bp)
CAAGCAGAAGACGGCATACGAGATATTGGCTCGTGGCCGATGTTTCGCAT	381076	0.1109018188039721	Illumina PCR Primer Index 6 (96% over 30bp)
CAAGCAGAAGACGGCATACGAGATCCGACAACGTGGCCGATGTTTCGCAT	378466	0.11014224919822845	Illumina PCR Primer Index 9 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATGTACGCAAGTGGCCGATGTTTCGCAT	374819	0.10908088891004948	RNA PCR Primer, Index 16 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATATCATTCCGTGGCCGATGTTTCGCAT	372798	0.10849273175556368	TruSeq Adapter, Index 25 (96% over 30bp)
TTCTAAGTGTGTAGATCTCGGTGGTCGCCGTATCATTAGCTGAGACTGCCAAGGCACACAGGGGATA	371018	0.10797471110490327	Illumina Single End PCR Primer 1 (96% over 33bp)
CAAGCAGAAGACGGCATACGAGATCGAACTTAGTGGCCGATGTTTCGCAT	365779	0.10645004245950443	RNA PCR Primer, Index 14 (96% over 30bp)
CAAGCAGAAGACGGCATACGAGATCCTCTATCGTGGCCGATGTTTCGCAT	364963	0.10621256782414548	RNA PCR Primer, Index 23 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATAAGGACACGTGGCCGATGTTTCGCAT	361621	0.10523996950138868	Illumina PCR Primer Index 10 (96% over 27bp)
TTCTAAGTGTGTAGATCTCGGTGGTCGCCGTATCATTAGCTGAGACTGCC	359673	0.1046730570140367	Illumina Single End PCR Primer 1 (96% over 33bp)
CAAGCAGAAGACGGCATACGAGATGAACAGGCGTGGCCGATGTTTCGCAT	355446	0.10344290348013693	Illumina PCR Primer Index 7 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATCAATGGAAGTGGCCGATGTTTCGCAT	355281	0.10339488471195774	Illumina PCR Primer Index 5 (96% over 29bp)
TTCTAAGTGTGTAGATCTCGGTGGTCGCCGTATCATTAGCTGAGACTGCCAGGCACACAGGGGATAGGC	348269	0.10135423257576064	Illumina Single End PCR Primer 1 (96% over 33bp)
CAAGCAGAAGACGGCATACGAGATAGGCTAACGTGGCCGATGTTTCGCAT	345326	0.10049775236514624	Illumina PCR Primer Index 10 (96% over 30bp)

Adapter Content

Adapter graph

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content