stdin:025999-L470_D6_D10_RNA_CL13-UGAv3-122

Basic Statistics

Measure	Value
Filename	025999-L470_D6_D10_RNA_CL13-UGAv3-122_trimmed
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	236513878
Sequences flagged as poor quality	0
Sequence length	9-579
%GC	49

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CAAGCAGAAGACGGCATACGAGATAGATCGCAGTGGCCGATGTTTCGCAT	554377	0.23439512500826698	Illumina PCR Primer Index 2 (96% over 30bp)
CAAGCAGAAGACGGCATACGAGATAGTCACTAGTGGCCGATGTTTCGCAT	550412	0.2327186906131572	RNA PCR Primer, Index 37 (96% over 28bp)
CCTTCATCAGGGTTTGCTGAAGATGGCGGTATATAGGCTGAGCAAGAGGT	531550	0.22474368290557561	No Hit
CAAGCAGAAGACGGCATACGAGATCTCAATGAGTGGCCGATGTTTCGCAT	495319	0.2094249200886216	RNA PCR Primer, Index 17 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATCCAGTTCAGTGGCCGATGTTTCGCAT	489079	0.20678659710615374	RNA PCR Primer, Index 23 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATACACAGAAGTGGCCGATGTTTCGCAT	477762	0.20200167704323888	RNA PCR Primer, Index 23 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATACAGCAGAGTGGCCGATGTTTCGCAT	470251	0.19882596487636128	Illumina PCR Primer Index 2 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATAACCGAGAGTGGCCGATGTTTCGCAT	464062	0.19620920511057705	Illumina PCR Primer Index 10 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATCACTTCGAGTGGCCGATGTTTCGCAT	457052	0.19324531983700338	Illumina PCR Primer Index 5 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATCCGTGAGAGTGGCCGATGTTTCGCAT	456221	0.19289396624751126	Illumina Paired End PCR Primer 2 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATCCGAAGTAGTGGCCGATGTTTCGCAT	446354	0.18872211803148398	Illumina PCR Primer Index 9 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATATCCTGTAGTGGCCGATGTTTCGCAT	441986	0.18687529194375646	RNA PCR Primer, Index 31 (96% over 30bp)
CAAGCAGAAGACGGCATACGAGATAGTACAAGGTGGCCGATGTTTCGCAT	439414	0.185787829329829	RNA PCR Primer, Index 22 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATCGCTGATCGTGGCCGATGTTTCGCAT	434886	0.18387335393485874	Illumina PCR Primer Index 5 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATCGCATACAGTGGCCGATGTTTCGCAT	429662	0.18166460405338244	RNA PCR Primer, Index 33 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATAGCAGGAAGTGGCCGATGTTTCGCAT	429320	0.18152000365915102	TruSeq Adapter, Index 25 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATACCTCCAAGTGGCCGATGTTTCGCAT	423374	0.17900598627874176	Illumina PCR Primer Index 2 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATAACGTGATGTGGCCGATGTTTCGCAT	422042	0.17844280579594574	RNA PCR Primer, Index 31 (96% over 30bp)
CAAGCAGAAGACGGCATACGAGATCAGCGTTAGTGGCCGATGTTTCGCAT	417501	0.17652283389476198	Illumina PCR Primer Index 10 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATCATACCAAGTGGCCGATGTTTCGCAT	415824	0.17581378459322372	RNA PCR Primer, Index 22 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATAAGGTACAGTGGCCGATGTTTCGCAT	403950	0.17079336037947002	Illumina PCR Primer Index 10 (96% over 30bp)
CAAGCAGAAGACGGCATACGAGATACACGACCGTGGCCGATGTTTCGCAT	399463	0.1688962201194807	RNA PCR Primer, Index 23 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATACAAGCTAGTGGCCGATGTTTCGCAT	396953	0.1678349716121098	Illumina PCR Primer Index 8 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATCAGATCTGGTGGCCGATGTTTCGCAT	393783	0.1664946697123625	Illumina PCR Primer Index 9 (96% over 30bp)
CAAGCAGAAGACGGCATACGAGATCAACCACAGTGGCCGATGTTTCGCAT	393475	0.1663644447959202	RNA PCR Primer, Index 23 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATCATCAAGTGTGGCCGATGTTTCGCAT	390033	0.16490913907386018	Illumina PCR Primer Index 7 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATGATAGACAGTGGCCGATGTTTCGCAT	380425	0.1608467981739321	RNA PCR Primer, Index 24 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATAACAACCAGTGGCCGATGTTTCGCAT	377399	0.1595673806507033	Illumina PCR Primer Index 12 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATAAACATCGGTGGCCGATGTTTCGCAT	375280	0.15867145013790693	Illumina PCR Primer Index 10 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATACGCTCGAGTGGCCGATGTTTCGCAT	375050	0.15857420425874544	Illumina PCR Primer Index 10 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATCACCTTACGTGGCCGATGTTTCGCAT	370878	0.15681024857239032	RNA PCR Primer, Index 33 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATGCGAGTAAGTGGCCGATGTTTCGCAT	366177	0.15482262736396382	RNA PCR Primer, Index 24 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATTGGTGGTAGTGGCCGATGTTTCGCAT	365697	0.15461967944223554	RNA PCR Primer, Index 36 (96% over 30bp)
CAAGCAGAAGACGGCATACGAGATCAAGACTAGTGGCCGATGTTTCGCAT	361974	0.15304556462433042	RNA PCR Primer, Index 21 (96% over 27bp)
CAAGCAGAAGACGGCATACGAGATCTGAGCCAGTGGCCGATGTTTCGCAT	359760	0.15210946733535863	Illumina PCR Primer Index 9 (96% over 30bp)
GTGTAGATCTCGGTGGTCGCCGTATCATTAGCTGAGACTGCCAAGGCACACAGGG	358668	0.1516477608134268	Illumina Single End PCR Primer 1 (100% over 29bp)
CAAGCAGAAGACGGCATACGAGATACGTATCAGTGGCCGATGTTTCGCAT	358148	0.15142790056488778	Illumina PCR Primer Index 2 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATCGACTGGAGTGGCCGATGTTTCGCAT	358045	0.15138435132335026	RNA PCR Primer, Index 33 (96% over 31bp)
CAAGCAGAAGACGGCATACGAGATAGTGGTCAGTGGCCGATGTTTCGCAT	352450	0.14901873961070478	Illumina PCR Primer Index 6 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATTCCGTCTAGTGGCCGATGTTTCGCAT	349590	0.14780950824374034	RNA PCR Primer, Index 40 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATACATTGGCGTGGCCGATGTTTCGCAT	349274	0.14767590086193588	Illumina PCR Primer Index 2 (96% over 31bp)
CAAGCAGAAGACGGCATACGAGATACAGATTCGTGGCCGATGTTTCGCAT	348049	0.14715796085335847	Illumina PCR Primer Index 2 (96% over 27bp)
CAAGCAGAAGACGGCATACGAGATAGCACCTCGTGGCCGATGTTTCGCAT	346895	0.14667004022487	TruSeq Adapter, Index 25 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATAGATGTACGTGGCCGATGTTTCGCAT	341717	0.14448073951922602	Illumina PCR Primer Index 2 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATAACTCACCGTGGCCGATGTTTCGCAT	338262	0.14301993729095253	Illumina PCR Primer Index 5 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATACCACTGTGTGGCCGATGTTTCGCAT	337220	0.14257937117753403	TruSeq Adapter, Index 25 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATGTCTGTCAGTGGCCGATGTTTCGCAT	336928	0.14245591119181597	Illumina PCR Primer Index 3 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATCCTCCTGAGTGGCCGATGTTTCGCAT	335154	0.14170584949776183	RNA PCR Primer, Index 23 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATGCTCGGTAGTGGCCGATGTTTCGCAT	332615	0.1406323395534532	Illumina PCR Primer Index 7 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATGCTAACGAGTGGCCGATGTTTCGCAT	332321	0.1405080339513946	RNA PCR Primer, Index 24 (96% over 31bp)
CAAGCAGAAGACGGCATACGAGATTATCAGCAGTGGCCGATGTTTCGCAT	331212	0.1400391396905682	RNA PCR Primer, Index 19 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATATGCCTAAGTGGCCGATGTTTCGCAT	324824	0.13733824109890075	RNA PCR Primer, Index 37 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATTTCACGCAGTGGCCGATGTTTCGCAT	324758	0.13731033575966312	RNA PCR Primer, Index 13 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATATTGAGGAGTGGCCGATGTTTCGCAT	323144	0.13662792337285173	Illumina PCR Primer Index 6 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATGTCGTAGAGTGGCCGATGTTTCGCAT	321740	0.13603430070179645	RNA PCR Primer, Index 41 (96% over 31bp)
CAAGCAGAAGACGGCATACGAGATTAGGATGAGTGGCCGATGTTTCGCAT	321620	0.1359835637213644	RNA PCR Primer, Index 46 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATGAATCTGAGTGGCCGATGTTTCGCAT	317497	0.13424032563535235	RNA PCR Primer, Index 39 (96% over 28bp)
CCCTATCGGTGTAGATCTCGGTGGTCGCCGTATCATTAGCTGAGACTGCCAAGGCACACAGGGGATAGGC	316914	0.13399382847208652	Illumina Single End PCR Primer 1 (96% over 30bp)
CAAGCAGAAGACGGCATACGAGATCTGGCATAGTGGCCGATGTTTCGCAT	316521	0.13382766486117148	RNA PCR Primer, Index 30 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATAACGCTTAGTGGCCGATGTTTCGCAT	312662	0.13219604813211003	RNA PCR Primer, Index 31 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATCAAGGAGCGTGGCCGATGTTTCGCAT	311648	0.13176732064745902	RNA PCR Primer, Index 21 (96% over 27bp)
CAAGCAGAAGACGGCATACGAGATCCATCCTCGTGGCCGATGTTTCGCAT	305830	0.12930742271284393	Illumina PCR Primer Index 2 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATGGAGAACAGTGGCCGATGTTTCGCAT	300683	0.12713122906047822	Illumina PCR Primer Index 11 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATGTGTTCTAGTGGCCGATGTTTCGCAT	298750	0.12631394086735157	RNA PCR Primer, Index 39 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATGACTAGTAGTGGCCGATGTTTCGCAT	296372	0.12530850303845595	Illumina PCR Primer Index 3 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATACTATGCAGTGGCCGATGTTTCGCAT	285285	0.12062082885470256	RNA PCR Primer, Index 44 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATATTGGCTCGTGGCCGATGTTTCGCAT	283904	0.12003693077156344	Illumina PCR Primer Index 6 (96% over 30bp)
CAAGCAGAAGACGGCATACGAGATTGGAACAAGTGGCCGATGTTTCGCAT	282609	0.1194893941910673	RNA PCR Primer, Index 27 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATAGAGTCAAGTGGCCGATGTTTCGCAT	279454	0.11815543441387401	RNA PCR Primer, Index 32 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATTGAAGAGAGTGGCCGATGTTTCGCAT	277306	0.11724724246413988	Illumina PCR Primer Index 8 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATTCTTCACAGTGGCCGATGTTTCGCAT	274919	0.11623799936171188	RNA PCR Primer, Index 36 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATGCCACATAGTGGCCGATGTTTCGCAT	271156	0.11464697221699607	RNA PCR Primer, Index 24 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATCTAAGGTCGTGGCCGATGTTTCGCAT	263857	0.11156089538221516	Illumina PCR Primer Index 9 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATATCATTCCGTGGCCGATGTTTCGCAT	262898	0.11115542234692882	TruSeq Adapter, Index 25 (96% over 30bp)
CAAGCAGAAGACGGCATACGAGATTGGCTTCAGTGGCCGATGTTTCGCAT	259529	0.10973098162129835	RNA PCR Primer, Index 15 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATAAGAGATCGTGGCCGATGTTTCGCAT	257689	0.10895301458800655	RNA PCR Primer, Index 27 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATCCTAATCCGTGGCCGATGTTTCGCAT	256838	0.10859320483510909	RNA PCR Primer, Index 22 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATCGAACTTAGTGGCCGATGTTTCGCAT	255331	0.10795603292251628	RNA PCR Primer, Index 14 (96% over 30bp)
CAAGCAGAAGACGGCATACGAGATCGACACACGTGGCCGATGTTTCGCAT	248240	0.10495790018715095	RNA PCR Primer, Index 21 (96% over 30bp)
CAAGCAGAAGACGGCATACGAGATGTACGCAAGTGGCCGATGTTTCGCAT	248068	0.10488517718186499	RNA PCR Primer, Index 16 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATGAACAGGCGTGGCCGATGTTTCGCAT	246950	0.10441247764750615	Illumina PCR Primer Index 7 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATAAGGACACGTGGCCGATGTTTCGCAT	246666	0.10429240012715027	Illumina PCR Primer Index 10 (96% over 27bp)
CAAGCAGAAGACGGCATACGAGATGGTGCGAAGTGGCCGATGTTTCGCAT	245675	0.10387339723041536	Illumina PCR Primer Index 1 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATGAGCTGAAGTGGCCGATGTTTCGCAT	243563	0.10298042637481086	Illumina PCR Primer Index 7 (96% over 30bp)
CAAGCAGAAGACGGCATACGAGATCTGTAGCCGTGGCCGATGTTTCGCAT	239276	0.10116784774887502	RNA PCR Primer, Index 17 (96% over 29bp)

Adapter Content

Adapter graph

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content