stdin:025999-L466_D6_D10_RNA_CL09-UGAv3-214

Basic Statistics

Measure	Value
Filename	025999-L466_D6_D10_RNA_CL09-UGAv3-214_trimmed
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	400402627
Sequences flagged as poor quality	0
Sequence length	8-434
%GC	50

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CAAGCAGAAGACGGCATACGAGATAGATCGCAGTGGCCGATGTTTCGCAT	980363	0.24484429768738755	Illumina PCR Primer Index 2 (96% over 30bp)
CAAGCAGAAGACGGCATACGAGATACAGCAGAGTGGCCGATGTTTCGCAT	911569	0.22766309173091415	Illumina PCR Primer Index 2 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATAACGTGATGTGGCCGATGTTTCGCAT	870974	0.21752454686067782	RNA PCR Primer, Index 31 (96% over 30bp)
CAAGCAGAAGACGGCATACGAGATCTCAATGAGTGGCCGATGTTTCGCAT	867963	0.21677255379246052	RNA PCR Primer, Index 17 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATACACAGAAGTGGCCGATGTTTCGCAT	861186	0.21508000745459643	RNA PCR Primer, Index 23 (96% over 28bp)
CCTTCATCAGGGTTTGCTGAAGATGGCGGTATATAGGCTGAGCAAGAGGT	856001	0.2137850609057068	No Hit
CTGCGCGTGTGTAGATCTCGGTGGTCGCCGTATCATTAGCTGAGACTGCCAAGGCACACAGGGGATA	822147	0.20533007142333257	Illumina RNA PCR Primer (96% over 30bp)
CAAGCAGAAGACGGCATACGAGATCATACCAAGTGGCCGATGTTTCGCAT	820272	0.20486179277739852	RNA PCR Primer, Index 22 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATACCTCCAAGTGGCCGATGTTTCGCAT	810982	0.20254162817967725	Illumina PCR Primer Index 2 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATACGCTCGAGTGGCCGATGTTTCGCAT	810964	0.20253713270467627	Illumina PCR Primer Index 10 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATAGTCACTAGTGGCCGATGTTTCGCAT	809185	0.20209282992541405	RNA PCR Primer, Index 37 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATACAAGCTAGTGGCCGATGTTTCGCAT	785740	0.19623747373665457	Illumina PCR Primer Index 8 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATAGATGTACGTGGCCGATGTTTCGCAT	778058	0.19431890490568635	Illumina PCR Primer Index 2 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATCGCATACAGTGGCCGATGTTTCGCAT	774706	0.19348174756106182	RNA PCR Primer, Index 33 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATAGCAGGAAGTGGCCGATGTTTCGCAT	754759	0.18850001201415695	TruSeq Adapter, Index 25 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATAAGGTACAGTGGCCGATGTTTCGCAT	750813	0.1875145039945005	Illumina PCR Primer Index 10 (96% over 30bp)
CAAGCAGAAGACGGCATACGAGATCAGCGTTAGTGGCCGATGTTTCGCAT	748024	0.1868179551179618	Illumina PCR Primer Index 10 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATCCAGTTCAGTGGCCGATGTTTCGCAT	745891	0.1862852413303472	RNA PCR Primer, Index 23 (96% over 29bp)
GTGTAGATCTCGGTGGTCGCCGTATCATTAGCTGAGACTGCCAAGGCACACAGGGGATAGGC	737219	0.1841194213743258	Illumina Single End PCR Primer 1 (100% over 29bp)
CAAGCAGAAGACGGCATACGAGATACGTATCAGTGGCCGATGTTTCGCAT	737208	0.18411667413960298	Illumina PCR Primer Index 2 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATACAGATTCGTGGCCGATGTTTCGCAT	724590	0.18096534616392515	Illumina PCR Primer Index 2 (96% over 27bp)
CAAGCAGAAGACGGCATACGAGATCCATCCTCGTGGCCGATGTTTCGCAT	724504	0.18094386778336496	Illumina PCR Primer Index 2 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATATCCTGTAGTGGCCGATGTTTCGCAT	720480	0.17993887937203767	RNA PCR Primer, Index 31 (96% over 30bp)
CAAGCAGAAGACGGCATACGAGATAACCGAGAGTGGCCGATGTTTCGCAT	710855	0.1775350489895762	Illumina PCR Primer Index 10 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATACACGACCGTGGCCGATGTTTCGCAT	708945	0.17705802914225136	RNA PCR Primer, Index 23 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATCACTTCGAGTGGCCGATGTTTCGCAT	701766	0.175265083862699	Illumina PCR Primer Index 5 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATCAGATCTGGTGGCCGATGTTTCGCAT	701407	0.17517542411129086	Illumina PCR Primer Index 9 (96% over 30bp)
CAAGCAGAAGACGGCATACGAGATCACCTTACGTGGCCGATGTTTCGCAT	682060	0.17034353773108488	RNA PCR Primer, Index 33 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATTGGTGGTAGTGGCCGATGTTTCGCAT	678162	0.16937001764476436	RNA PCR Primer, Index 36 (96% over 30bp)
CTGCGCGTGTGTAGATCTCGGTGGTCGCCGTATCATTAGCTGAGACTGCCAAGGCACACAGGGGATAGGC	676593	0.16897816207384675	Illumina RNA PCR Primer (96% over 30bp)
CAAGCAGAAGACGGCATACGAGATCTAAGGTCGTGGCCGATGTTTCGCAT	674316	0.16840948448622442	Illumina PCR Primer Index 9 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATATGCCTAAGTGGCCGATGTTTCGCAT	660369	0.1649262406063085	RNA PCR Primer, Index 37 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATCAAGGAGCGTGGCCGATGTTTCGCAT	657235	0.16414352845891794	RNA PCR Primer, Index 21 (96% over 27bp)
CAAGCAGAAGACGGCATACGAGATAGTACAAGGTGGCCGATGTTTCGCAT	653221	0.1631410375337023	RNA PCR Primer, Index 22 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATACATTGGCGTGGCCGATGTTTCGCAT	651915	0.16281486584752103	Illumina PCR Primer Index 2 (96% over 31bp)
CAAGCAGAAGACGGCATACGAGATTATCAGCAGTGGCCGATGTTTCGCAT	642361	0.16042876761645222	RNA PCR Primer, Index 19 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATCGCTGATCGTGGCCGATGTTTCGCAT	641523	0.16021947828029612	Illumina PCR Primer Index 5 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATCATCAAGTGTGGCCGATGTTTCGCAT	641033	0.16009710146082531	Illumina PCR Primer Index 7 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATCCGTGAGAGTGGCCGATGTTTCGCAT	640501	0.15996423519968564	Illumina Paired End PCR Primer 2 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATAACAACCAGTGGCCGATGTTTCGCAT	640006	0.15984060963715904	Illumina PCR Primer Index 12 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATAAACATCGGTGGCCGATGTTTCGCAT	638816	0.15954340878987291	Illumina PCR Primer Index 10 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATAGTGGTCAGTGGCCGATGTTTCGCAT	638607	0.15949121133013944	Illumina PCR Primer Index 6 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATGCTCGGTAGTGGCCGATGTTTCGCAT	638457	0.1594537490384647	Illumina PCR Primer Index 7 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATGCTAACGAGTGGCCGATGTTTCGCAT	638217	0.15939380937178516	RNA PCR Primer, Index 24 (96% over 31bp)
CAAGCAGAAGACGGCATACGAGATGTCGTAGAGTGGCCGATGTTTCGCAT	637245	0.15915105372173294	RNA PCR Primer, Index 41 (96% over 31bp)
CAAGCAGAAGACGGCATACGAGATCAACCACAGTGGCCGATGTTTCGCAT	636364	0.15893102519529675	RNA PCR Primer, Index 23 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATCGACTGGAGTGGCCGATGTTTCGCAT	635874	0.15880864837582595	RNA PCR Primer, Index 33 (96% over 31bp)
CAAGCAGAAGACGGCATACGAGATCTGAGCCAGTGGCCGATGTTTCGCAT	624902	0.15606840661412544	Illumina PCR Primer Index 9 (96% over 30bp)
CAAGCAGAAGACGGCATACGAGATGCGAGTAAGTGGCCGATGTTTCGCAT	624558	0.15598249309188472	RNA PCR Primer, Index 24 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATAACTCACCGTGGCCGATGTTTCGCAT	623194	0.1556418359862559	Illumina PCR Primer Index 5 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATTCCGTCTAGTGGCCGATGTTTCGCAT	615817	0.15379944048169295	RNA PCR Primer, Index 40 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATCCGAAGTAGTGGCCGATGTTTCGCAT	609379	0.15219155892301375	Illumina PCR Primer Index 9 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATGTCTGTCAGTGGCCGATGTTTCGCAT	601075	0.150117646455901	Illumina PCR Primer Index 3 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATTAGGATGAGTGGCCGATGTTTCGCAT	599235	0.1496581090113577	RNA PCR Primer, Index 46 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATTTCACGCAGTGGCCGATGTTTCGCAT	597026	0.14910641432929458	RNA PCR Primer, Index 13 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATATTGAGGAGTGGCCGATGTTTCGCAT	586773	0.14654574181902158	Illumina PCR Primer Index 6 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATGAATCTGAGTGGCCGATGTTTCGCAT	586169	0.14639489365787803	RNA PCR Primer, Index 39 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATCAAGACTAGTGGCCGATGTTTCGCAT	582182	0.14539914594516384	RNA PCR Primer, Index 21 (96% over 27bp)
CAAGCAGAAGACGGCATACGAGATCTGGCATAGTGGCCGATGTTTCGCAT	578377	0.14444885247968164	RNA PCR Primer, Index 30 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATCCTCCTGAGTGGCCGATGTTTCGCAT	574899	0.14358022681005037	RNA PCR Primer, Index 23 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATAACGCTTAGTGGCCGATGTTTCGCAT	572802	0.14305650397243772	RNA PCR Primer, Index 31 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATTGGAACAAGTGGCCGATGTTTCGCAT	554624	0.13851657371868342	RNA PCR Primer, Index 27 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATACCACTGTGTGGCCGATGTTTCGCAT	554314	0.13843915164922233	TruSeq Adapter, Index 25 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATAAGACGGAGTGGCCGATGTTTCGCAT	538217	0.13441894825530204	RNA PCR Primer, Index 27 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATGTGTTCTAGTGGCCGATGTTTCGCAT	537491	0.13423763076359638	RNA PCR Primer, Index 39 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATGGAGAACAGTGGCCGATGTTTCGCAT	532203	0.13291696010775672	Illumina PCR Primer Index 11 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATAGCACCTCGTGGCCGATGTTTCGCAT	531528	0.13274837979522047	TruSeq Adapter, Index 25 (96% over 28bp)
CTGCGCGTGTGTAGATCTCGGTGGTCGCCGTATCATTAGCTGAGACTGCCAAGGCACACAGGGGATAG	531065	0.13263274618825116	Illumina RNA PCR Primer (96% over 30bp)
CAAGCAGAAGACGGCATACGAGATGATAGACAGTGGCCGATGTTTCGCAT	527786	0.13181382049224166	RNA PCR Primer, Index 24 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATGCCACATAGTGGCCGATGTTTCGCAT	514284	0.12844171474429414	RNA PCR Primer, Index 24 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATACTATGCAGTGGCCGATGTTTCGCAT	511748	0.1278083522663801	RNA PCR Primer, Index 44 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATCTGTAGCCGTGGCCGATGTTTCGCAT	510612	0.12752463784409687	RNA PCR Primer, Index 17 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATTGGCTTCAGTGGCCGATGTTTCGCAT	508179	0.1269169994731328	RNA PCR Primer, Index 15 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATGACTAGTAGTGGCCGATGTTTCGCAT	503271	0.12569123328953585	Illumina PCR Primer Index 3 (96% over 29bp)
CTGCGCGTGTGTAGATCTCGGTGGTCGCCGTATCATTAGCTGAGACTGCCAGGCACACAGGGGATAGGC	502359	0.1254634625561535	Illumina RNA PCR Primer (96% over 30bp)
CAAGCAGAAGACGGCATACGAGATATCATTCCGTGGCCGATGTTTCGCAT	502299	0.1254484776394836	TruSeq Adapter, Index 25 (96% over 30bp)
CAAGCAGAAGACGGCATACGAGATATTGGCTCGTGGCCGATGTTTCGCAT	499930	0.12485682317963413	Illumina PCR Primer Index 6 (96% over 30bp)
CAAGCAGAAGACGGCATACGAGATGAGCTGAAGTGGCCGATGTTTCGCAT	499336	0.12470847250460222	Illumina PCR Primer Index 7 (96% over 30bp)
GTGTAGATCTCGGTGGTCGCCGTATCATTAGCTGAGACTGCCAGGCACACAGGGGATAGGC	491275	0.12269524895000253	Illumina Single End PCR Primer 1 (100% over 29bp)
CAAGCAGAAGACGGCATACGAGATTCTTCACAGTGGCCGATGTTTCGCAT	488667	0.12204390457208464	RNA PCR Primer, Index 36 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATAGAGTCAAGTGGCCGATGTTTCGCAT	478948	0.11961659782017366	RNA PCR Primer, Index 32 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATAATGTTGCGTGGCCGATGTTTCGCAT	475832	0.11883838114778403	Illumina PCR Primer Index 6 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATCCGACAACGTGGCCGATGTTTCGCAT	473042	0.11814158252263415	Illumina PCR Primer Index 9 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATTGAAGAGAGTGGCCGATGTTTCGCAT	467720	0.11681242041401492	Illumina PCR Primer Index 8 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATGAACAGGCGTGGCCGATGTTTCGCAT	465714	0.11631142470001828	Illumina PCR Primer Index 7 (96% over 28bp)
CTGCGCGTGTGTAGATCTCGGTGGTCGCCGTATCATTAGCTGAGACTGCCAGGCACACAGGGGATA	464876	0.11610213536386213	Illumina RNA PCR Primer (96% over 30bp)
CAAGCAGAAGACGGCATACGAGATGACAGTGCGTGGCCGATGTTTCGCAT	462333	0.11546702464566996	Illumina PCR Primer Index 12 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATGGTGCGAAGTGGCCGATGTTTCGCAT	460471	0.11500199273168105	Illumina PCR Primer Index 1 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATAAGGACACGTGGCCGATGTTTCGCAT	453358	0.1132255308604656	Illumina PCR Primer Index 10 (96% over 27bp)
GTGTAGATCTCGGTGGTCGCCGTATCATTAGCTGAGACTGCCAAGGCACACAGGGGATA	450902	0.11261214827144478	Illumina Single End PCR Primer 1 (100% over 29bp)
CAAGCAGAAGACGGCATACGAGATCGACACACGTGGCCGATGTTTCGCAT	446856	0.11160166539067187	RNA PCR Primer, Index 21 (96% over 30bp)
GTGTAGATCTCGGTGGTCGCCGTATCATTAGCTGAGACTGCCAAGGCACACAGGGGATAG	435088	0.10866262373448413	Illumina Single End PCR Primer 1 (100% over 29bp)
CAAGCAGAAGACGGCATACGAGATAAGAGATCGTGGCCGATGTTTCGCAT	427004	0.10664365596182765	RNA PCR Primer, Index 27 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATCGAACTTAGTGGCCGATGTTTCGCAT	421476	0.10526304563930845	RNA PCR Primer, Index 14 (96% over 30bp)
CAAGCAGAAGACGGCATACGAGATGTACGCAAGTGGCCGATGTTTCGCAT	419426	0.10475106098642055	RNA PCR Primer, Index 16 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATCCTAATCCGTGGCCGATGTTTCGCAT	414058	0.10341041044168774	RNA PCR Primer, Index 22 (96% over 28bp)

Adapter Content

Adapter graph

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content