LOCUS       4_04              4280 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             complement(3297..4157)
                     /note="pLannotate"
                     /label="AmpR"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             2253..3047
                     /note="pLannotate"
                     /label="NeoR/KanR"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      1..589
                     /note="pLannotate"
                     /label="ori"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     CDS             1602..1904
                     /note="pLannotate"
                     /label="ccdB"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     promoter        complement(4158..4210)
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="73.61111111111111"
                     /fragment="True"
                     /other="promoter"
     promoter        3145..3204
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="58.82352941176471"
                     /fragment="True"
                     /other="promoter"
     promoter        913..943
                     /note="pLannotate"
                     /label="lac promoter"
                     /database="addgene"
                     /identity="96.875"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     misc_feature    3068..3124
                     /note="pLannotate"
                     /label="bom"
                     /database="addgene"
                     /identity="98.246"
                     /match_length="40.42553191489361"
                     /fragment="True"
                     /other="misc feature"
     protein_bind    complement(947..969)
                     /note="pLannotate"
                     /label="lac operator"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="95.83333333333334"
                     /fragment="False"
                     /other="protein bind"
     protein_bind    complement(877..898)
                     /note="pLannotate"
                     /label="CAP binding site"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein bind"
     misc_feature    complement(1116..1134)
                     /note="pLannotate"
                     /label="Tn5 ME"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc feature"
     promoter        complement(1423..1441)
                     /note="pLannotate"
                     /label="T7 promoter"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     promoter        1012..1030
                     /note="pLannotate"
                     /label="T3 promoter"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             771..864
                     /note="pLannotate"
                     /label="lacI"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="8.679593721144968"
                     /fragment="True"
                     /other="CDS"
     CDS             1444..1591
                     /note="pLannotate"
                     /label="lacZ"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="4.813008130081301"
                     /fragment="True"
                     /other="CDS"
     rep_origin      3050..3067
                     /note="pLannotate"
                     /label="f1 ori"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="3.9735099337748347"
                     /fragment="True"
                     /other="rep_origin"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctca gaattaaccc
     1021 tcactaaagg gactagtcct gcaggtttaa acgaattcgc ccttaatgat acggcgacca
     1081 ccgagatcta cacaggttgc ctcgtcggca gcgtcagatg tgtataagag acagcaataa
     1141 actctatttt ccatgttctc agggcccctg ggtagacaga cacagcttga tttcagagca
     1201 gacatagaca aaaaaaaaaa aaatagcaat tccctttctc ttatacacat ctccgagccc
     1261 acgagactcg gacgatcatg ggtaccgacc aatgcagcgc gctcaagcac gtggattgac
     1321 agattcagtc gtacgccgat gcgaaacatc ggccactatg tggcatctcg tatgccgtct
     1381 tctgcttgaa gggcgaattc gcggccgcta aattcaattc gccctatagt gagtcgtatt
     1441 acaattcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac
     1501 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca
     1561 ccgatcgccc ttcccaacag ttgcgcagcc tatacgtacg gcagtttaag gtttacacct
     1621 ataaaagaga gagccgttat cgtctgtttg tggatgtaca gagtgatatt attgacacgc
     1681 cggggcgacg gatggtgatc cccctggcca gtgcacgtct gctgtcagat aaagtctccc
     1741 gtgaacttta cccggtggtg catatcgggg atgaaagctg gcgcatgatg accaccgata
     1801 tggccagtgt gccggtctcc gttatcgggg aagaagtggc tgatctcagc caccgcgaaa
     1861 atgacatcaa aaacgccatt aacctgatgt tctggggaat ataaatgtca ggcatgagat
     1921 tatcaaaaag gatcttcacc tagatccttt tcacgtagaa agccagtccg cagaaacggt
     1981 gctgaccccg gatgaatgtc agctactggg ctatctggac aagggaaaac gcaagcgcaa
     2041 agagaaagca ggtagcttgc agtgggctta catggcgata gctagactgg gcggttttat
     2101 ggacagcaag cgaaccggaa ttgccagctg gggcgccctc tggtaaggtt gggaagccct
     2161 gcaaagtaaa ctggatggct ttcttgccgc caaggatctg atggcgcagg ggatcaagct
     2221 ctgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag
     2281 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg
     2341 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca
     2401 agaccgacct gtccggtgcc ctgaatgaac tgcaagacga ggcagcgcgg ctatcgtggc
     2461 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg
     2521 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg
     2581 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta
     2641 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag
     2701 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac
     2761 tgttcgccag gctcaaggcg agcatgcccg acggcgagga tctcgtcgtg acccatggcg
     2821 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg
     2881 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg
     2941 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg
     3001 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgaatt attaacgctt
     3061 acaatttcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatca
     3121 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     3181 tcaaatatgt atccgctcat gagattatca aaaaggatct tcacctagat ccttttaaat
     3241 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     3301 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     3361 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     3421 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     3481 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     3541 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     3601 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     3661 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     3721 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     3781 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     3841 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     3901 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     3961 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     4021 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     4081 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     4141 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     4201 tgtctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     4261 gaaaagatca aaggatcttc
//
