LOCUS       3_03              4354 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             complement(3371..4231)
                     /note="pLannotate"
                     /label="AmpR"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             2327..3121
                     /note="pLannotate"
                     /label="NeoR/KanR"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      1..589
                     /note="pLannotate"
                     /label="ori"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     CDS             1676..1978
                     /note="pLannotate"
                     /label="ccdB"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     promoter        complement(4232..4284)
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="73.61111111111111"
                     /fragment="True"
                     /other="promoter"
     promoter        3219..3278
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="58.82352941176471"
                     /fragment="True"
                     /other="promoter"
     promoter        913..943
                     /note="pLannotate"
                     /label="lac promoter"
                     /database="addgene"
                     /identity="96.875"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     misc_feature    3142..3198
                     /note="pLannotate"
                     /label="bom"
                     /database="addgene"
                     /identity="98.246"
                     /match_length="40.42553191489361"
                     /fragment="True"
                     /other="misc feature"
     protein_bind    complement(947..969)
                     /note="pLannotate"
                     /label="lac operator"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="95.83333333333334"
                     /fragment="False"
                     /other="protein bind"
     protein_bind    complement(877..898)
                     /note="pLannotate"
                     /label="CAP binding site"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein bind"
     misc_feature    1393..1411
                     /note="pLannotate"
                     /label="Tn5 ME"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc feature"
     misc_feature    complement(1203..1221)
                     /note="pLannotate"
                     /label="Tn5 ME"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc feature"
     promoter        1012..1030
                     /note="pLannotate"
                     /label="T3 promoter"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     promoter        complement(1497..1515)
                     /note="pLannotate"
                     /label="T7 promoter"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             771..864
                     /note="pLannotate"
                     /label="lacI"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="8.679593721144968"
                     /fragment="True"
                     /other="CDS"
     CDS             1518..1665
                     /note="pLannotate"
                     /label="lacZ"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="4.813008130081301"
                     /fragment="True"
                     /other="CDS"
     rep_origin      3124..3141
                     /note="pLannotate"
                     /label="f1 ori"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="3.9735099337748347"
                     /fragment="True"
                     /other="rep_origin"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctca gaattaaccc
     1021 tcactaaagg gactagtcct gcaggtttaa acgaattcgc ccttcaagca gaagacggca
     1081 tacgagatac acagaagtgg ccgatgtttc gcatcggcgt acgactgaca gtgcatccac
     1141 gtgcttgagc gcgctgcata cttgctaagg tccccatgat cgtccgagtc tcgtgggctc
     1201 ggagatgtgt ataagagaca gggggggggt ctgctacttg tggacatgga taagggttac
     1261 caacatggcc agtaagtgaa agtttactgg aagtttttgt gaaaaatgag tcatgaggtt
     1321 gatgccaggc aagttttcag gtcaaagaga agatgtggtt attgacatca ggtaagtgca
     1381 gggtcttagt agctgtctct tatacacatc tgacgctgcc gacgaggcaa cctgtgtaga
     1441 tctcggtggt cgccgtatca ttaagggcga attcgcggcc gctaaattca attcgcccta
     1501 tagtgagtcg tattacaatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc
     1561 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
     1621 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctatacg tacggcagtt
     1681 taaggtttac acctataaaa gagagagccg ttatcgtctg tttgtggatg tacagagtga
     1741 tattattgac acgccggggc gacggatggt gatccccctg gccagtgcac gtctgctgtc
     1801 agataaagtc tcccgtgaac tttacccggt ggtgcatatc ggggatgaaa gctggcgcat
     1861 gatgaccacc gatatggcca gtgtgccggt ctccgttatc ggggaagaag tggctgatct
     1921 cagccaccgc gaaaatgaca tcaaaaacgc cattaacctg atgttctggg gaatataaat
     1981 gtcaggcatg agattatcaa aaaggatctt cacctagatc cttttcacgt agaaagccag
     2041 tccgcagaaa cggtgctgac cccggatgaa tgtcagctac tgggctatct ggacaaggga
     2101 aaacgcaagc gcaaagagaa agcaggtagc ttgcagtggg cttacatggc gatagctaga
     2161 ctgggcggtt ttatggacag caagcgaacc ggaattgcca gctggggcgc cctctggtaa
     2221 ggttgggaag ccctgcaaag taaactggat ggctttcttg ccgccaagga tctgatggcg
     2281 caggggatca agctctgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga
     2341 tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc
     2401 acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc
     2461 ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcaag acgaggcagc
     2521 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac
     2581 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc
     2641 tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac
     2701 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg
     2761 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct
     2821 cgcgccagcc gaactgttcg ccaggctcaa ggcgagcatg cccgacggcg aggatctcgt
     2881 cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg
     2941 attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac
     3001 ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg
     3061 tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg
     3121 aattattaac gcttacaatt tcctgatgcg gtattttctc cttacgcatc tgtgcggtat
     3181 ttcacaccgc atcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     3241 ttttctaaat acattcaaat atgtatccgc tcatgagatt atcaaaaagg atcttcacct
     3301 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     3361 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     3421 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     3481 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     3541 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     3601 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     3661 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     3721 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     3781 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     3841 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     3901 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     3961 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     4021 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     4081 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     4141 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     4201 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     4261 tttatcaggg ttattgtctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     4321 cgtcagaccc cgtagaaaag atcaaaggat cttc
//
