LOCUS       1_01              4560 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             complement(3577..4437)
                     /note="pLannotate"
                     /label="AmpR"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             2533..3327
                     /note="pLannotate"
                     /label="NeoR/KanR"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      1..589
                     /note="pLannotate"
                     /label="ori"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     CDS             1882..2184
                     /note="pLannotate"
                     /label="ccdB"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     promoter        complement(4438..4490)
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="73.61111111111111"
                     /fragment="True"
                     /other="promoter"
     promoter        3425..3484
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="58.82352941176471"
                     /fragment="True"
                     /other="promoter"
     promoter        913..943
                     /note="pLannotate"
                     /label="lac promoter"
                     /database="addgene"
                     /identity="96.875"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     misc_feature    3348..3404
                     /note="pLannotate"
                     /label="bom"
                     /database="addgene"
                     /identity="98.246"
                     /match_length="40.42553191489361"
                     /fragment="True"
                     /other="misc feature"
     protein_bind    complement(947..969)
                     /note="pLannotate"
                     /label="lac operator"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="95.83333333333334"
                     /fragment="False"
                     /other="protein bind"
     protein_bind    complement(877..898)
                     /note="pLannotate"
                     /label="CAP binding site"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein bind"
     misc_feature    complement(1203..1221)
                     /note="pLannotate"
                     /label="Tn5 ME"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc feature"
     misc_feature    1602..1620
                     /note="pLannotate"
                     /label="Tn5 ME"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc feature"
     promoter        complement(1703..1721)
                     /note="pLannotate"
                     /label="T7 promoter"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     promoter        1012..1030
                     /note="pLannotate"
                     /label="T3 promoter"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             771..864
                     /note="pLannotate"
                     /label="lacI"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="8.679593721144968"
                     /fragment="True"
                     /other="CDS"
     CDS             1724..1871
                     /note="pLannotate"
                     /label="lacZ"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="4.813008130081301"
                     /fragment="True"
                     /other="CDS"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctca gaattaaccc
     1021 tcactaaagg gactagtcct gcaggtttaa acgaattcgc ccttcaagca gaagacggca
     1081 tacgagatcg actggagtgg ccgatgtttc gcatcggcgt acgactattg gctcatccac
     1141 gtgcttgagc gcgctgcata cttggaacag gccccatgat cgtccgagtc tcgtgggctc
     1201 ggagatgtgt ataagagaca gccggctctc gttttttttt ttttttttgg gaggaattta
     1261 gtttctagtt ttaccttaaa gcaaagatga taatagccct tcccaaaact aaattgcctt
     1321 tgtaaaacta gtgaaaggtc acaaggttag gattatgaga ggggcctcaa ttctgataag
     1381 ataggcatag tttctacaat cccttaccgc tcaggagtca tgtggccaga ggtcacaaga
     1441 ttgctgactt ccccaattgc tcctatagat aacttcacta ttgtagaacc taagattggt
     1501 tttttgaaat atctttcaga ctaaccccat tcagactcgc tactcatgac tcaactgatc
     1561 ctgtggcccc acccagaggc agactcagtg cacaaggacc actgtctctt atacacatct
     1621 gacgctgcga cgaggcaacc tgtgtagatc tcggtggtcg ccgtatcaaa gggcgaattc
     1681 gcggccgcta aattcaattc gccctatagt gagtcgtatt acaattcact ggccgtcgtt
     1741 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
     1801 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
     1861 ttgcgcagcc tatacgtacg gcagtttaag gtttacacct ataaaagaga gagccgttat
     1921 cgtctgtttg tggatgtaca gagtgatatt attgacacgc cggggcgacg gatggtgatc
     1981 cccctggcca gtgcacgtct gctgtcagat aaagtctccc gtgaacttta cccggtggtg
     2041 catatcgggg atgaaagctg gcgcatgatg accaccgata tggccagtgt gccggtctcc
     2101 gttatcgggg aagaagtggc tgatctcagc caccgcgaaa atgacatcaa aaacgccatt
     2161 aacctgatgt tctggggaat ataaatgtca ggcatgagat tatcaaaaag gatcttcacc
     2221 tagatccttt tcacgtagaa agccagtccg cagaaacggt gctgaccccg gatgaatgtc
     2281 agctactggg ctatctggac aagggaaaac gcaagcgcaa agagaaagca ggtagcttgc
     2341 agtgggctta catggcgata gctagactgg gcggttttat ggacagcaag cgaaccggaa
     2401 ttgccagctg gggcgccctc tggtaaggtt gggaagccct gcaaagtaaa ctggatggct
     2461 ttcttgccgc caaggatctg atggcgcagg ggatcaagct ctgatcaaga gacaggatga
     2521 ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg
     2581 gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg
     2641 ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc
     2701 ctgaatgaac tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct
     2761 tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa
     2821 gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg
     2881 gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa
     2941 gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat
     3001 gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg
     3061 agcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc
     3121 atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac
     3181 cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg
     3241 gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc
     3301 tatcgccttc ttgacgagtt cttctgaatt attaacgctt acaatttcct gatgcggtat
     3361 tttctcctta cgcatctgtg cggtatttca caccgcatca ggtggcactt ttcggggaaa
     3421 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     3481 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     3541 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     3601 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     3661 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     3721 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     3781 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     3841 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     3901 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     3961 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     4021 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     4081 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     4141 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     4201 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     4261 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     4321 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     4381 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     4441 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga ccaaaatccc
     4501 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
//
