LOCUS 1_01 4105 bp DNA circular UNK 01-JAN-1980 DEFINITION . ACCESSION VERSION KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers CDS complement(3122..3982) /note="pLannotate" /label="AmpR" /database="addgene" /identity="100.0" /match_length="100.0" /fragment="False" /other="CDS" CDS 2078..2872 /note="pLannotate" /label="NeoR/KanR" /database="addgene" /identity="100.0" /match_length="100.0" /fragment="False" /other="CDS" rep_origin 1..589 /note="pLannotate" /label="ori" /database="addgene" /identity="100.0" /match_length="100.0" /fragment="False" /other="rep_origin" CDS 1427..1729 /note="pLannotate" /label="ccdB" /database="addgene" /identity="100.0" /match_length="100.0" /fragment="False" /other="CDS" promoter complement(3983..4035) /note="pLannotate" /label="AmpR promoter" /database="addgene" /identity="100.0" /match_length="73.61111111111111" /fragment="True" /other="promoter" promoter 2970..3029 /note="pLannotate" /label="AmpR promoter" /database="addgene" /identity="100.0" /match_length="58.82352941176471" /fragment="True" /other="promoter" promoter 913..943 /note="pLannotate" /label="lac promoter" /database="addgene" /identity="96.875" /match_length="100.0" /fragment="False" /other="promoter" misc_feature 2893..2949 /note="pLannotate" /label="bom" /database="addgene" /identity="98.246" /match_length="40.42553191489361" /fragment="True" /other="misc feature" protein_bind complement(947..969) /note="pLannotate" /label="lac operator" /database="addgene" /identity="100.0" /match_length="95.83333333333334" /fragment="False" /other="protein bind" protein_bind complement(877..898) /note="pLannotate" /label="CAP binding site" /database="addgene" /identity="100.0" /match_length="100.0" /fragment="False" /other="protein bind" promoter complement(1248..1266) /note="pLannotate" /label="T7 promoter" /database="addgene" /identity="100.0" /match_length="100.0" /fragment="False" /other="promoter" promoter 1012..1030 /note="pLannotate" /label="T3 promoter" /database="addgene" /identity="100.0" /match_length="100.0" /fragment="False" /other="promoter" CDS 771..864 /note="pLannotate" /label="lacI" /database="addgene" /identity="100.0" /match_length="8.679593721144968" /fragment="True" /other="CDS" CDS 1269..1416 /note="pLannotate" /label="lacZ" /database="addgene" /identity="100.0" /match_length="4.813008130081301" /fragment="True" /other="CDS" rep_origin 2875..2892 /note="pLannotate" /label="f1 ori" /database="addgene" /identity="100.0" /match_length="3.9735099337748347" /fragment="True" /other="rep_origin" ORIGIN 1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc 61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt 121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt 181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc 241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa 301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac 361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg 421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga 481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact 541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa 601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc 661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg 721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat 781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt 841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta 901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg 961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctca gaattaaccc 1021 tcactaaagg gactagtcct gcaggtttaa acgaattcgc ccttcaagca gaagacggca 1081 tacgagatcc gaagtagtgg ccgatgtttc gcatcggcgt acgactatgc ctaaatccac 1141 gtgctgtctc ttataaacat ctgacgctgc cgacgattgg tacggtgtag atctcggtgg 1201 tcgccgtatc attaagggcg aattcgcggc cgctaaattc aattcgccct atagtgagtc 1261 gtattacaat tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac 1321 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc 1381 ccgcaccgat cgcccttccc aacagttgcg cagcctatac gtacggcagt ttaaggttta 1441 cacctataaa agagagagcc gttatcgtct gtttgtggat gtacagagtg atattattga 1501 cacgccgggg cgacggatgg tgatccccct ggccagtgca cgtctgctgt cagataaagt 1561 ctcccgtgaa ctttacccgg tggtgcatat cggggatgaa agctggcgca tgatgaccac 1621 cgatatggcc agtgtgccgg tctccgttat cggggaagaa gtggctgatc tcagccaccg 1681 cgaaaatgac atcaaaaacg ccattaacct gatgttctgg ggaatataaa tgtcaggcat 1741 gagattatca aaaaggatct tcacctagat ccttttcacg tagaaagcca gtccgcagaa 1801 acggtgctga ccccggatga atgtcagcta ctgggctatc tggacaaggg aaaacgcaag 1861 cgcaaagaga aagcaggtag cttgcagtgg gcttacatgg cgatagctag actgggcggt 1921 tttatggaca gcaagcgaac cggaattgcc agctggggcg ccctctggta aggttgggaa 1981 gccctgcaaa gtaaactgga tggctttctt gccgccaagg atctgatggc gcaggggatc 2041 aagctctgat caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca 2101 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac 2161 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt 2221 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa gacgaggcag cgcggctatc 2281 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg 2341 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc 2401 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc 2461 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat 2521 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc 2581 cgaactgttc gccaggctca aggcgagcat gcccgacggc gaggatctcg tcgtgaccca 2641 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga 2701 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat 2761 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc 2821 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gaattattaa 2881 cgcttacaat ttcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg 2941 catcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa 3001 tacattcaaa tatgtatccg ctcatgagat tatcaaaaag gatcttcacc tagatccttt 3061 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca 3121 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca 3181 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc 3241 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa 3301 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc 3361 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca 3421 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat 3481 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag 3541 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac 3601 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt 3661 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt 3721 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc 3781 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat 3841 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca 3901 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga 3961 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg 4021 gttattgtct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc 4081 ccgtagaaaa gatcaaagga tcttc //