LOCUS       2_02              5053 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             complement(4070..4930)
                     /note="pLannotate"
                     /label="AmpR"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             3026..3820
                     /note="pLannotate"
                     /label="NeoR/KanR"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      1..589
                     /note="pLannotate"
                     /label="ori"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     CDS             2375..2677
                     /note="pLannotate"
                     /label="ccdB"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     promoter        complement(4931..4983)
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="73.61111111111111"
                     /fragment="True"
                     /other="promoter"
     promoter        3918..3977
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="58.82352941176471"
                     /fragment="True"
                     /other="promoter"
     promoter        913..943
                     /note="pLannotate"
                     /label="lac promoter"
                     /database="addgene"
                     /identity="96.875"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     misc_feature    3841..3897
                     /note="pLannotate"
                     /label="bom"
                     /database="addgene"
                     /identity="98.246"
                     /match_length="40.42553191489361"
                     /fragment="True"
                     /other="misc feature"
     protein_bind    complement(947..969)
                     /note="pLannotate"
                     /label="lac operator"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="95.83333333333334"
                     /fragment="False"
                     /other="protein bind"
     protein_bind    complement(877..898)
                     /note="pLannotate"
                     /label="CAP binding site"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein bind"
     misc_feature    complement(1203..1221)
                     /note="pLannotate"
                     /label="Tn5 ME"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc feature"
     misc_feature    2092..2110
                     /note="pLannotate"
                     /label="Tn5 ME"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc feature"
     promoter        complement(2196..2214)
                     /note="pLannotate"
                     /label="T7 promoter"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     promoter        1012..1030
                     /note="pLannotate"
                     /label="T3 promoter"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             771..864
                     /note="pLannotate"
                     /label="lacI"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="8.679593721144968"
                     /fragment="True"
                     /other="CDS"
     CDS             2217..2364
                     /note="pLannotate"
                     /label="lacZ"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="4.813008130081301"
                     /fragment="True"
                     /other="CDS"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctca gaattaaccc
     1021 tcactaaagg gactagtcct gcaggtttaa acgaattcgc ccttcaagca gaagacggca
     1081 tacgagatga ctagtagtgg ccgatgtttc gcatcggcgt acgactgtcg tagaatccac
     1141 gtgcttgagc gcgctgcata cttgcgaact tacccatgat cgtccgagtc tcgtgggctc
     1201 ggagatgtgt ataagagaca ggatccactc tctaacatca gcttagattt tttttttttt
     1261 tttttttgag acggaatctc actctgctgc ccaggcggga gtgccgtggc gcaatctcag
     1321 ctcactacaa cctccgcctc ccgggttcaa gggattctcc tgcctcagcc tcctgagtag
     1381 gtgggattac aggcacccgc caccaggggc agctaatttt tttttttttt ttgagacagg
     1441 gtcttgctct gttgcccagg ctggagtgca gtggcacgat ctcggctcac tgcaagctcc
     1501 acctcccggg ttcacgccac tctcctgcct tagcctccca agtagctggg actacaggca
     1561 cccgccacca cagccggcta atttttttgt atttttagtg gagacggggt ttcaccgtgg
     1621 tagtcaggat ggtctcgatc tcctgacctc gtgatctgcc tgcctcggcc tcccaaagtg
     1681 ctgggattac aggcttgagc caccgcacct gccctaattt ttttattttt agtagcgaca
     1741 ggggtctcac cacgttggcc aggctggtct tgaactcctg aactcaagtg atccgcccgc
     1801 ctcggcctcc caaaatgctg ggattacagg cgtgagccac tgcgccggcc taacttagat
     1861 ttttacaagg agagagagag atggcagagc aaaaatgccc atgtaactta gtacaaaaca
     1921 gtactaagaa aaatactaat ttattcttaa tctccaccta atagtaagga aaagaggaga
     1981 ggaggactta acaataagtt tgtaagtagc acaaattatg aggggtctag ggattgcatt
     2041 tcagggaagg aatgacatta ctatgttcct tccatattag aaatcttaag gctgtctctt
     2101 atacacatct gacgctgccg acgaatagag aggtgtagat ctcggtggtc gccgtatcat
     2161 taagggcgaa ttcgcggccg ctaaattcaa ttcgccctat agtgagtcgt attacaattc
     2221 actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg
     2281 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg
     2341 cccttcccaa cagttgcgca gcctatacgt acggcagttt aaggtttaca cctataaaag
     2401 agagagccgt tatcgtctgt ttgtggatgt acagagtgat attattgaca cgccggggcg
     2461 acggatggtg atccccctgg ccagtgcacg tctgctgtca gataaagtct cccgtgaact
     2521 ttacccggtg gtgcatatcg gggatgaaag ctggcgcatg atgaccaccg atatggccag
     2581 tgtgccggtc tccgttatcg gggaagaagt ggctgatctc agccaccgcg aaaatgacat
     2641 caaaaacgcc attaacctga tgttctgggg aatataaatg tcaggcatga gattatcaaa
     2701 aaggatcttc acctagatcc ttttcacgta gaaagccagt ccgcagaaac ggtgctgacc
     2761 ccggatgaat gtcagctact gggctatctg gacaagggaa aacgcaagcg caaagagaaa
     2821 gcaggtagct tgcagtgggc ttacatggcg atagctagac tgggcggttt tatggacagc
     2881 aagcgaaccg gaattgccag ctggggcgcc ctctggtaag gttgggaagc cctgcaaagt
     2941 aaactggatg gctttcttgc cgccaaggat ctgatggcgc aggggatcaa gctctgatca
     3001 agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc
     3061 ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc
     3121 tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga
     3181 cctgtccggt gccctgaatg aactgcaaga cgaggcagcg cggctatcgt ggctggccac
     3241 gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct
     3301 gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa
     3361 agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc
     3421 attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct
     3481 tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc
     3541 caggctcaag gcgagcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg
     3601 cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct
     3661 gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct
     3721 tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca
     3781 gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga attattaacg cttacaattt
     3841 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tcaggtggca
     3901 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     3961 tgtatccgct catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     4021 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     4081 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     4141 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     4201 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     4261 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     4321 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     4381 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     4441 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     4501 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     4561 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     4621 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     4681 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     4741 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     4801 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     4861 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     4921 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     4981 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     5041 tcaaaggatc ttc
//
