LOCUS       barcode_recov_expected   477 bp ds-DNA     linear       22-OCT-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    1..29
                     /label="illumina P5"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    30..37
                     /label="i5"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    38..70
                     /label="TruSeq R1"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    38..100
                     /label="oBL026"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    71..100
                     /label="oBL011' minus 1"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    115..133
                     /label="variant barcode"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    185..280
                     /label="bGH polyA partial"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    complement(186..205)
                     /label="oBL012"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    281..310
                     /label="polyA"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    311..320
                     /label="UMI"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    321..354
                     /label="Nextera R2"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    355..453
                     /label="cell barcode"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    complement(454..477)
                     /label="illumina P7"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
ORIGIN
        1 AATGATACGG CGACCACCGA GATCTACACT ATAGCCTACA CTCTTTCCCT ACACGACGCT
       61 CTTCCGATCT ggtaccaagc ttaAGTTTAA ACCGTGACGA TCGATCGATC GATGBNSWNS
      121 KMSVNBSWNM KYNgtggtgt taattaacct agagggcccg tttaaacccg ctgatcagcc
      181 tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg
      241 accctggaag gtgccactcc cactgtcctt tcctaataaa AAAAAAAAAA AAAAAAAAAA
      301 AAAAAAAAAA NNNNNNNNNN CTGTCTCTTA TACACATCTC CGAGCCCACG AGACTCGGAC
      361 GATCATGGGN NNNNNNNCAA GTATGCAGCG CGCTCAAGCA CGTGGATNNN NNNNNAGTCG
      421 TACGCCGATG CGAAACATCG GCCACNNNNN NNNATCTCGT ATGCCGTCTT CTGCTTG
//