## htsjdk.samtools.metrics.StringHeader
# CollectQualityYieldMetricsFlow INCLUDE_BQ_HISTOGRAM=true INPUT=/Ddata/Runs/441525/output/441525-20260312_0210/441525-L5417-Z0114-CAACATACATCAGAT/441525-L5417-Z0114-CAACATACATCAGAT_sample.sam.gz OUTPUT=/Ddata/Runs/441525/output/441525-20260312_0210/441525-L5417-Z0114-CAACATACATCAGAT/441525-L5417-Z0114-CAACATACATCAGAT_FlowQ.metric    INCLUDE_SECONDARY_ALIGNMENTS=false INCLUDE_SUPPLEMENTAL_ALIGNMENTS=false IGNORET0TAG=false FILLINGVALUE=0.0 KEEPBOUNDARYFLOWS=false ASSUME_SORTED=true STOP_AFTER=0 VERBOSITY=INFO QUIET=false VALIDATION_STRINGENCY=STRICT COMPRESSION_LEVEL=5 MAX_RECORDS_IN_RAM=500000 CREATE_INDEX=false CREATE_MD5_FILE=false USE_JDK_DEFLATER=false USE_JDK_INFLATER=false
## htsjdk.samtools.metrics.StringHeader
# Started on: Fri Mar 13 02:52:35 EDT 2026

## METRICS CLASS	picard.analysis.CollectQualityYieldMetricsFlow$QualityYieldMetricsFlow
TOTAL_READS	PF_READS	MEAN_READ_LENGTH_IN_FLOWS	PF_FLOWS	PF_Q20_FLOWS	PCT_PF_Q20_FLOWS	PF_Q30_FLOWS	PCT_PF_Q30_FLOWS	Q20_EQUIVALENT_YIELD	PF_Q20_EQUIVALENT_YIELD
1000148	1000148	174	174686141	142307483	0.814647	115077790	0.658769	252543655	252543655

## HISTOGRAM	java.lang.Integer
KEY	QUAL_COUNT	MEAN_CYCLE_QUAL	MEDIAN_CYCLE_QUAL	Q25_CYCLE_QUAL	Q75_CYCLE_QUAL
0	2029571	26.238202	26.25	26.25	26.25
1	223963	34.227151	34.25	34.25	35
2	2779	34.682662	35	34.25	35
3	1173262	34.012543	34.25	34.25	35
4	5305	31.759276	31.75	30	33.5
5	7176	34.761724	35	35	35
6	2817453	32.944412	34.25	32.5	35
7	9086	32.103656	32.5	30	35
8	12661	33.219787	33.75	32.5	35
9	4887215	33.47982	34.25	32.5	35
10	5918	34.197376	35	34.25	35
11	13277	32.102697	33.75	31.25	35
12	5830118	32.45342	35	31.75	35
13	33174	32.483793	34.25	31.75	35
14	592555	32.588564	35	31.75	35
15	4272157	32.543603	35	31.75	35
16	573257	32.613408	35	31.75	35
17	4817711	32.548132	35	31.75	35
18	93	32.518736	35	31.75	35
19	5071927	32.448448	34.25	31.75	35
20	256289	32.327054	34.25	31.75	35
21	219014	32.053845	34.25	31	35
22	2822654	31.611956	34.25	30.25	35
23	0	30.928886	33.75	29.25	35
24	26404	29.972262	33.75	27.75	35
25	23373712	28.628998	32.5	25	35
26	0	27.872369	32.5	22.5	35
27	7	26.95621	31.75	18.75	35
28	136910	26.11723	30.25	17	35
29	394703	25.457069	29	16.25	35
30	3968476	24.935779	26.75	16	34.25
31	0	24.548385	25	16	34.25
32	8121877	24.155039	25	16	33.75
33	0	23.846013	24.5	16.25	33
34	0	23.61068	23.5	16.25	32.5
35	102987437	23.283847	23.5	16.25	31.75
36	0	23.16129	23	16.25	31.75
37	0	22.835708	22.75	16.25	31
38	0	22.620326	22.5	16	30
39	0	22.441322	22.25	16	30
40	0	22.161843	22	15.5	29.75
41	0	22.01525	21.75	15.5	29.25
42	0	21.812492	21.5	15.25	29.25
43	0	21.672951	21.25	15.25	28.5
44	0	21.601664	21	15	28.5
45	0	21.538618	21	15	28.5
46	0	21.555279	21	15	28.25
47	0	21.657932	21.5	15.25	28
48	0	21.749296	21.5	15.5	28
49	0	21.747781	21.5	15.5	27.75
50	0	21.714883	21.5	15.5	27.5
51	0	21.641598	21.5	15.5	27.5
52	0	21.691003	21.5	15.75	27.5
53	0	21.62826	21.5	15.75	27.5
54	0	21.504992	21.25	15.75	27.5
55	0	21.449758	21	15.5	27.5
56	0	21.583053	21.5	15.75	27.5
57	0	21.631448	21.5	15.75	27.5
58	0	21.87778	22	16	27.5
59	0	21.967464	22	16.25	27.75
60	0	22.107026	22.5	16.5	28.25
61	0	22.22142	22.5	16.5	28.5
62	0	22.451256	23	16.75	29
63	0	22.727735	23.5	17	29.25
64	0	22.996762	23.5	17.5	30
65	0	23.227894	23.5	17.75	30
66	0	23.471361	25	18	30
67	0	23.670048	25	18.25	30
68	0	23.849059	25	18.25	30.25
69	0	24.027073	25	18.5	31
70	0	24.320839	25	19	31.25
71	0	24.632657	25	19.5	31.75
72	0	24.78077	25.25	19.75	31.75
73	0	24.967442	26	20.25	31.75
74	0	25.15517	26	20.5	31.75
75	0	25.243853	26	20.25	31.75
76	0	25.147144	26	20	31.75
77	0	25.177317	26.75	19.75	31.75
78	0	25.231397	26.75	20	32.5
79	0	25.148436	27	19.75	32.5
80	0	25.276778	27	20	32.5
81	0	25.572486	27.5	20.5	32.5
82	0	25.676541	27.5	21	32.5
83	0	25.77081	27.5	20.75	32.5
84	0	25.75091	27.5	21	32.5
85	0	25.69608	27.5	20.5	32.5
86	0	25.720033	27.5	20.5	32.5
87	0	25.449711	27	20	32.5
88	0	25.544807	27.5	20.25	32.5
89	0	25.563131	27.5	20	32.5
90	0	25.572885	27.5	20	32.5
91	0	25.365598	26.75	19.5	32.5
92	0	25.546788	27.5	19.75	32.5
93	0	25.568454	27.25	19.75	33
94	0	25.474131	26.75	19.5	33.75
95	0	25.447858	27	19.5	33.5
96	0	25.443748	26.75	19.5	33.75
97	0	25.335515	26.75	19.25	33.5
98	0	25.324463	26.75	19.5	33.75
99	0	25.086179	26.5	18.75	33.75
100	0	25.158697	26.25	18.75	34.25
101	0	24.687953	25.25	18	33.5
102	0	24.838734	26	18	34.25
103	0	24.788724	25.25	17.75	33.5
104	0	24.492114	25	17.5	33.5
105	0	23.399404	22.75	16.25	32.5
106	0	22.209954	21.75	14.5	31
107	0	20.619215	19.5	13.25	27.5
108	0	12.658087	11.333333	6	17.5