## htsjdk.samtools.metrics.StringHeader
# CollectQualityYieldMetricsFlow INCLUDE_BQ_HISTOGRAM=true INPUT=/Ddata/Runs/441525/output/441525-20260312_0210/441525-L5416-Z0113-CAGTTCATCTGTGAT/441525-L5416-Z0113-CAGTTCATCTGTGAT_sample.sam.gz OUTPUT=/Ddata/Runs/441525/output/441525-20260312_0210/441525-L5416-Z0113-CAGTTCATCTGTGAT/441525-L5416-Z0113-CAGTTCATCTGTGAT_FlowQ.metric    INCLUDE_SECONDARY_ALIGNMENTS=false INCLUDE_SUPPLEMENTAL_ALIGNMENTS=false IGNORET0TAG=false FILLINGVALUE=0.0 KEEPBOUNDARYFLOWS=false ASSUME_SORTED=true STOP_AFTER=0 VERBOSITY=INFO QUIET=false VALIDATION_STRINGENCY=STRICT COMPRESSION_LEVEL=5 MAX_RECORDS_IN_RAM=500000 CREATE_INDEX=false CREATE_MD5_FILE=false USE_JDK_DEFLATER=false USE_JDK_INFLATER=false
## htsjdk.samtools.metrics.StringHeader
# Started on: Fri Mar 13 02:52:29 EDT 2026

## METRICS CLASS	picard.analysis.CollectQualityYieldMetricsFlow$QualityYieldMetricsFlow
TOTAL_READS	PF_READS	MEAN_READ_LENGTH_IN_FLOWS	PF_FLOWS	PF_Q20_FLOWS	PCT_PF_Q20_FLOWS	PF_Q30_FLOWS	PCT_PF_Q30_FLOWS	Q20_EQUIVALENT_YIELD	PF_Q20_EQUIVALENT_YIELD
1001983	1001983	197	197803268	164704139	0.832666	135023250	0.682614	290724898	290724898

## HISTOGRAM	java.lang.Integer
KEY	QUAL_COUNT	MEAN_CYCLE_QUAL	MEDIAN_CYCLE_QUAL	Q25_CYCLE_QUAL	Q75_CYCLE_QUAL
0	2035969	26.243178	26.25	26.25	26.25
1	211762	34.38872	35	34.25	35
2	3904	34.596464	35	34.25	35
3	1194824	33.879983	34.25	32.5	35
4	7920	31.606885	31.75	30	33.5
5	9498	34.763512	35	35	35
6	2810439	33.227911	34.25	32.5	35
7	11566	32.337726	33.5	30.25	35
8	15419	33.297109	33.75	32.5	35
9	4863126	33.444689	34.25	32.5	35
10	6928	34.248995	35	34.25	35
11	15402	32.360665	33.75	31.75	35
12	5914447	32.799108	35	32.25	35
13	37051	32.710513	35	31.75	35
14	616560	32.807582	35	32.5	35
15	4374305	32.73819	35	31.75	35
16	608249	32.833586	35	32.5	35
17	4985778	32.750141	35	32.25	35
18	97	32.741005	35	32	35
19	5375885	32.707232	35	31.75	35
20	281283	32.650376	35	31.75	35
21	285011	32.527818	35	31.75	35
22	3161221	32.331471	34.25	31.75	35
23	0	32.004949	34.25	31.25	35
24	31957	31.527301	34.25	30.75	35
25	25223037	30.797917	33.75	29.75	35
26	0	30.308942	33.75	29	35
27	18	29.67525	33.75	27.5	35
28	187180	29.022681	33.5	25.5	35
29	511182	28.414035	33	23.5	35
30	4856498	27.860165	32.5	21	35
31	0	27.36207	31.75	19.75	35
32	8963985	26.880078	31.25	18.75	35
33	0	26.476459	30	18.5	35
34	0	26.165802	29.25	18.25	35
35	121202767	25.83367	27.5	18	35
36	0	25.701575	26.75	18	34.25
37	0	25.395106	25.25	17.75	34.25
38	0	25.16596	25	17.75	34.25
39	0	24.929801	25	17.75	33.75
40	0	24.626649	25	17.25	33.75
41	0	24.447707	25	17	33.75
42	0	24.233056	25	17	33.75
43	0	24.057953	25	16.75	33.5
44	0	23.925154	24.5	16.5	33.5
45	0	23.846865	24.5	16.5	33.5
46	0	23.760705	24	16.5	33
47	0	23.76791	23.75	16.5	33
48	0	23.77412	23.75	16.5	33
49	0	23.708446	23.5	16.666667	32.5
50	0	23.642501	23.5	16.666667	32.5
51	0	23.527667	23.5	16.75	32.5
52	0	23.487158	23.5	16.75	32.5
53	0	23.281978	23.333333	16.5	31.75
54	0	23.116611	23	16.5	31.75
55	0	22.920451	23	16.25	31.25
56	0	22.97097	23	16.5	31.25
57	0	22.915982	23	16.5	31
58	0	22.955928	23	16.75	30.25
59	0	22.875718	23	16.75	30
60	0	22.858079	23	17	30
61	0	22.875376	23	17	30
62	0	22.923791	23.5	17.25	30
63	0	23.020981	23.5	17.5	30
64	0	23.088867	23.5	17.75	29.75
65	0	23.232728	23.5	18	30
66	0	23.314399	24	18	30
67	0	23.363096	24.5	18.25	30
68	0	23.439986	25	18.25	30
69	0	23.610863	25	18.5	30
70	0	23.702531	25	18.5	30
71	0	23.73203	25	18.75	30
72	0	23.791093	25	19	30
73	0	23.882212	25	19.25	30
74	0	23.886639	25	19	30
75	0	23.788731	25	18.75	30
76	0	23.715572	25	18.5	30
77	0	23.595279	25	17.75	30
78	0	23.395589	25	17.5	30
79	0	23.369942	25	17	30
80	0	23.581568	25	17.75	30
81	0	23.72805	25	18	30
82	0	23.869986	25	18.25	30
83	0	24.000067	25	18.5	30
84	0	23.916857	25	18.5	30
85	0	23.951902	25	18.25	30
86	0	23.980465	25	18.5	30.25
87	0	23.844533	25	18	30.5
88	0	23.976553	25	18	31
89	0	23.948187	25	18.25	31
90	0	23.971851	25	18	31
91	0	24.094625	25	18.25	31
92	0	24.081439	25	18	31.75
93	0	24.324479	25.25	18.5	31.75
94	0	24.266215	25	18.25	31.75
95	0	24.137977	25	18	31.75
96	0	23.999044	25	17.75	31.75
97	0	23.889279	25	17.5	31.75
98	0	23.957153	25	17.75	31.75
99	0	23.766505	24.5	17.5	31.75
100	0	23.567501	24.25	17.25	31
101	0	23.279739	23.5	16.5	31
102	0	22.979704	23	16.5	30
103	0	22.828237	22.75	16.25	30
104	0	22.63512	22	16.25	29.75
105	0	21.820083	21	15.5	28.5
106	0	20.622729	19.75	14	26.75
107	0	19.16459	18.25	13	25
108	0	11.449704	10.333333	6	15.666667