## htsjdk.samtools.metrics.StringHeader
# CollectQualityYieldMetricsFlow INCLUDE_BQ_HISTOGRAM=true INPUT=/Ddata/Runs/441525/output/441525-20260312_0210/441525-L5414-Z0109-CAATCTGCATACGAT/441525-L5414-Z0109-CAATCTGCATACGAT_sample.sam.gz OUTPUT=/Ddata/Runs/441525/output/441525-20260312_0210/441525-L5414-Z0109-CAATCTGCATACGAT/441525-L5414-Z0109-CAATCTGCATACGAT_FlowQ.metric    INCLUDE_SECONDARY_ALIGNMENTS=false INCLUDE_SUPPLEMENTAL_ALIGNMENTS=false IGNORET0TAG=false FILLINGVALUE=0.0 KEEPBOUNDARYFLOWS=false ASSUME_SORTED=true STOP_AFTER=0 VERBOSITY=INFO QUIET=false VALIDATION_STRINGENCY=STRICT COMPRESSION_LEVEL=5 MAX_RECORDS_IN_RAM=500000 CREATE_INDEX=false CREATE_MD5_FILE=false USE_JDK_DEFLATER=false USE_JDK_INFLATER=false
## htsjdk.samtools.metrics.StringHeader
# Started on: Fri Mar 13 02:51:14 EDT 2026

## METRICS CLASS	picard.analysis.CollectQualityYieldMetricsFlow$QualityYieldMetricsFlow
TOTAL_READS	PF_READS	MEAN_READ_LENGTH_IN_FLOWS	PF_FLOWS	PF_Q20_FLOWS	PCT_PF_Q20_FLOWS	PF_Q30_FLOWS	PCT_PF_Q30_FLOWS	Q20_EQUIVALENT_YIELD	PF_Q20_EQUIVALENT_YIELD
999035	999035	200	199861229	166251222	0.831833	135183804	0.676388	292925396	292925396

## HISTOGRAM	java.lang.Integer
KEY	QUAL_COUNT	MEAN_CYCLE_QUAL	MEDIAN_CYCLE_QUAL	Q25_CYCLE_QUAL	Q75_CYCLE_QUAL
0	2030836	26.240286	26.25	26.25	26.25
1	213118	34.613498	35	34.25	35
2	4344	34.389047	35	34.25	35
3	1270253	33.807093	34.25	32.5	35
4	8244	31.241708	31.75	30	33.5
5	9974	34.686694	35	35	35
6	2892504	32.1757	32.5	30.25	35
7	11793	32.155784	32.5	30	35
8	15849	33.292576	33.75	32.5	35
9	4904462	33.274093	34.25	32.5	35
10	7436	34.112219	35	34.25	35
11	16058	32.353352	33.75	31.75	35
12	5963116	32.659655	35	31.75	35
13	39642	32.566364	34.25	31.75	35
14	624805	32.662566	35	31.75	35
15	4423105	32.633098	35	31.75	35
16	623351	32.695224	35	31.75	35
17	5050055	32.595263	35	31.75	35
18	110	32.597007	35	31.75	35
19	5500952	32.575244	35	31.75	35
20	295150	32.543171	35	31.75	35
21	301284	32.47131	34.25	31.75	35
22	3321211	32.323999	34.25	31.75	35
23	0	32.110674	34.25	31.25	35
24	34122	31.758505	34.25	31	35
25	26371527	31.167876	33.75	30	35
26	0	30.791237	33.75	29.75	35
27	26	30.224634	33.75	28.75	35
28	197948	29.611634	33.75	27.5	35
29	546150	28.977688	33.25	25.25	35
30	5026981	28.353119	32.5	23.333333	35
31	0	27.754601	32.5	21	35
32	9385918	27.156824	31.75	19.5	35
33	0	26.654183	30.5	18.5	35
34	0	26.266	29.75	18.25	35
35	120770905	25.894518	28	18	34.25
36	0	25.729485	27.25	18	34.25
37	0	25.411908	25.5	18	34.25
38	0	25.159599	25	17.75	33.75
39	0	24.88974	25	17.75	33.75
40	0	24.558588	25	17.25	33.75
41	0	24.319401	25	17.25	33.5
42	0	24.035786	25	17	33
43	0	23.819713	24.25	16.75	33
44	0	23.636571	23.5	16.5	32.5
45	0	23.508216	23.5	16.5	32.5
46	0	23.41902	23.5	16.25	32.5
47	0	23.416118	23.5	16.5	32.5
48	0	23.461208	23.5	16.5	32.5
49	0	23.425606	23.5	16.5	32.5
50	0	23.404513	23.5	16.5	32.25
51	0	23.313297	23.5	16.5	31.75
52	0	23.306895	23.5	16.75	31.75
53	0	23.14518	23	16.666667	31.75
54	0	22.983212	23	16.5	31.25
55	0	22.807517	23	16.25	31
56	0	22.907034	23	16.5	30.5
57	0	22.87922	23	16.75	30.25
58	0	22.985611	23.25	17	30
59	0	22.94271	23	17	30
60	0	22.929155	23.5	17	30
61	0	22.97328	23.5	17	30
62	0	23.037342	23.5	17.5	30
63	0	23.10812	23.5	17.75	30
64	0	23.189653	23.5	18	30
65	0	23.297753	24	18	30
66	0	23.399088	24.5	18.25	30
67	0	23.477065	25	18.25	30
68	0	23.587856	25	18.5	30
69	0	23.729586	25	18.5	30
70	0	23.797858	25	18.75	30
71	0	23.781718	25	18.75	30
72	0	23.82533	25	19	30
73	0	23.878129	25	19	30
74	0	23.897735	25	19	30
75	0	23.887501	25	19	30
76	0	23.693187	25	18.5	30
77	0	23.478461	25	17.75	30
78	0	23.305861	25	17.25	30
79	0	23.23655	25	17	30
80	0	23.429261	25	17.5	30
81	0	23.516619	25	17.75	30
82	0	23.519811	25	17.75	30
83	0	23.489969	25	17.75	30
84	0	23.58269	25	18	30
85	0	23.596931	25	18	30
86	0	23.45101	25	17.75	30
87	0	23.24307	25	17.25	30
88	0	23.181419	24.5	17	30
89	0	23.191152	24.5	17	30
90	0	23.166601	24.25	17.25	30
91	0	23.227546	24.25	17.25	30
92	0	23.267381	24.25	17.5	30
93	0	23.366728	24.5	17.25	30
94	0	23.261485	24	17.25	30
95	0	23.078171	23.75	17	30
96	0	23.033185	23.5	17	30
97	0	22.762592	23.5	16.5	29.75
98	0	22.71302	23.25	16.5	29.75
99	0	22.620631	23	16.75	29.25
100	0	22.289083	22.75	16.25	28.5
101	0	21.960695	22	16	27.75
102	0	21.426606	21.25	15.5	27.5
103	0	21.032434	21	15.25	26.75
104	0	20.699055	20.25	15	26
105	0	19.8424	19.5	14.25	25
106	0	18.810603	18.25	13.5	23.75
107	0	17.677161	17.25	12.5	22.75
108	0	10.164758	9.5	6	12.5