## htsjdk.samtools.metrics.StringHeader
# CollectQualityYieldMetricsFlow INCLUDE_BQ_HISTOGRAM=true INPUT=/Ddata/Runs/441525/output/441525-20260312_0210/441525-L5407-Z0097-CAGTCAGTTGCAGAT/441525-L5407-Z0097-CAGTCAGTTGCAGAT_sample.sam.gz OUTPUT=/Ddata/Runs/441525/output/441525-20260312_0210/441525-L5407-Z0097-CAGTCAGTTGCAGAT/441525-L5407-Z0097-CAGTCAGTTGCAGAT_FlowQ.metric    INCLUDE_SECONDARY_ALIGNMENTS=false INCLUDE_SUPPLEMENTAL_ALIGNMENTS=false IGNORET0TAG=false FILLINGVALUE=0.0 KEEPBOUNDARYFLOWS=false ASSUME_SORTED=true STOP_AFTER=0 VERBOSITY=INFO QUIET=false VALIDATION_STRINGENCY=STRICT COMPRESSION_LEVEL=5 MAX_RECORDS_IN_RAM=500000 CREATE_INDEX=false CREATE_MD5_FILE=false USE_JDK_DEFLATER=false USE_JDK_INFLATER=false
## htsjdk.samtools.metrics.StringHeader
# Started on: Fri Mar 13 02:52:23 EDT 2026

## METRICS CLASS	picard.analysis.CollectQualityYieldMetricsFlow$QualityYieldMetricsFlow
TOTAL_READS	PF_READS	MEAN_READ_LENGTH_IN_FLOWS	PF_FLOWS	PF_Q20_FLOWS	PCT_PF_Q20_FLOWS	PF_Q30_FLOWS	PCT_PF_Q30_FLOWS	Q20_EQUIVALENT_YIELD	PF_Q20_EQUIVALENT_YIELD
1000566	1000566	202	202602541	169510058	0.836663	139293217	0.68752	298757796	298757796

## HISTOGRAM	java.lang.Integer
KEY	QUAL_COUNT	MEAN_CYCLE_QUAL	MEDIAN_CYCLE_QUAL	Q25_CYCLE_QUAL	Q75_CYCLE_QUAL
0	2033105	26.24111	26.25	26.25	26.25
1	203277	34.254952	35	34.25	35
2	3968	34.480008	35	34.25	35
3	1201495	33.905151	34.25	32.5	35
4	8404	31.697175	31.75	30	33.5
5	9531	34.706131	35	35	35
6	2829362	33.082382	34.25	32.5	35
7	11312	32.38335	33.75	30.5	35
8	15219	33.148315	33.75	32.5	35
9	4833524	33.376854	34.25	32.5	35
10	6977	34.187738	35	34.25	35
11	15148	32.479568	33.75	31.75	35
12	5912178	32.855174	35	32.5	35
13	37191	32.712504	35	31.75	35
14	610832	32.814084	35	32.5	35
15	4381747	32.772524	35	32.5	35
16	603299	32.847679	35	32.5	35
17	4991047	32.765006	35	32.5	35
18	150	32.768558	35	32.5	35
19	5384717	32.750916	35	32.5	35
20	281946	32.686191	35	31.75	35
21	301371	32.611299	35	31.75	35
22	3226770	32.435759	35	31.75	35
23	0	32.207056	34.25	31.75	35
24	33720	31.810553	34.25	31.25	35
25	25620101	31.191583	34.25	30.25	35
26	0	30.821612	34.25	30	35
27	14	30.255906	33.75	29	35
28	206666	29.639036	33.75	27.5	35
29	546253	29.036499	33.5	25.25	35
30	5123317	28.450389	33	23.5	35
31	0	27.865552	32.5	21.25	35
32	9239825	27.303314	31.75	19.5	35
33	0	26.814783	31	18.75	35
34	0	26.448309	30	18.25	35
35	124930075	26.106166	29.25	18	35
36	0	25.945091	27.75	18	35
37	0	25.661722	26.75	18	34.25
38	0	25.486732	25.75	18	34.25
39	0	25.263828	25	17.75	34.25
40	0	24.994813	25	17.75	33.75
41	0	24.82285	25	17.5	33.75
42	0	24.615799	25	17.25	33.75
43	0	24.452962	25	17	33.75
44	0	24.346844	25	17	33.75
45	0	24.285461	25	17	33.75
46	0	24.232797	25	16.75	33.75
47	0	24.291922	25	17	33.75
48	0	24.365858	25	17	33.75
49	0	24.346001	25	17	33.75
50	0	24.345595	25	17	33.75
51	0	24.270722	25	17	33.75
52	0	24.249131	25	17.25	33.5
53	0	24.080465	25	17	33
54	0	23.909556	24.25	17	33
55	0	23.715617	23.5	16.75	32.5
56	0	23.740532	23.5	17	32.5
57	0	23.667372	23.5	17	32.5
58	0	23.68241	23.5	17.25	32.25
59	0	23.582139	23.5	17.25	31.75
60	0	23.495774	23.5	17.25	31.75
61	0	23.444237	23.5	17.25	31.25
62	0	23.436928	23.5	17.5	30.25
63	0	23.463973	23.5	17.75	30
64	0	23.503505	23.5	18	30
65	0	23.552852	24.25	18.25	30
66	0	23.61992	25	18.5	30
67	0	23.630782	25	18.5	30
68	0	23.689666	25	18.5	30
69	0	23.800809	25	18.75	30
70	0	23.852071	25	19	30
71	0	23.866021	25	19	30
72	0	23.852716	25	19	30
73	0	23.826891	25	19.25	30
74	0	23.840521	25	19.25	30
75	0	23.70307	25	18.75	30
76	0	23.589515	25	18.5	30
77	0	23.408835	25	17.75	30
78	0	23.150074	25	17.25	30
79	0	23.098968	25	17	30
80	0	23.183341	25	17	30
81	0	23.260038	25	17.5	30
82	0	23.36411	25	17.75	30
83	0	23.457015	25	18	30
84	0	23.426418	25	17.75	30
85	0	23.44378	25	17.75	30
86	0	23.167372	25	17.25	30
87	0	23.050876	24.5	17	30
88	0	23.045183	24.75	17	30
89	0	23.085189	24.5	17	30
90	0	23.058879	24.25	17	30
91	0	23.146302	24.5	17	30
92	0	23.320037	24.25	17.5	30
93	0	23.575627	25	17.75	30
94	0	23.470085	24.5	17.5	30
95	0	23.368871	24.25	17.5	30
96	0	23.281521	24.25	17.25	30
97	0	23.143082	23.75	17	30
98	0	23.029138	23.5	16.75	30
99	0	22.943776	23.5	17	29.25
100	0	22.630314	23	16.75	29.25
101	0	22.06791	22	16.25	28.5
102	0	21.633634	21.5	15.5	27.5
103	0	21.473186	21.25	15.5	27.5
104	0	21.069592	20.75	15.25	26.75
105	0	20.14691	19.5	14.5	25.25
106	0	19.203403	18.75	13.75	24.5
107	0	17.858651	17.5	12.75	22.75
108	0	10.134491	9.5	6	12.5