## htsjdk.samtools.metrics.StringHeader
# CollectQualityYieldMetricsFlow INCLUDE_BQ_HISTOGRAM=true INPUT=/Ddata/Runs/441525/output/441525-20260312_0210/441525-L5404-Z0093-CAATGACAGCTCGAT/441525-L5404-Z0093-CAATGACAGCTCGAT_sample.sam.gz OUTPUT=/Ddata/Runs/441525/output/441525-20260312_0210/441525-L5404-Z0093-CAATGACAGCTCGAT/441525-L5404-Z0093-CAATGACAGCTCGAT_FlowQ.metric    INCLUDE_SECONDARY_ALIGNMENTS=false INCLUDE_SUPPLEMENTAL_ALIGNMENTS=false IGNORET0TAG=false FILLINGVALUE=0.0 KEEPBOUNDARYFLOWS=false ASSUME_SORTED=true STOP_AFTER=0 VERBOSITY=INFO QUIET=false VALIDATION_STRINGENCY=STRICT COMPRESSION_LEVEL=5 MAX_RECORDS_IN_RAM=500000 CREATE_INDEX=false CREATE_MD5_FILE=false USE_JDK_DEFLATER=false USE_JDK_INFLATER=false
## htsjdk.samtools.metrics.StringHeader
# Started on: Fri Mar 13 02:51:42 EDT 2026

## METRICS CLASS	picard.analysis.CollectQualityYieldMetricsFlow$QualityYieldMetricsFlow
TOTAL_READS	PF_READS	MEAN_READ_LENGTH_IN_FLOWS	PF_FLOWS	PF_Q20_FLOWS	PCT_PF_Q20_FLOWS	PF_Q30_FLOWS	PCT_PF_Q30_FLOWS	Q20_EQUIVALENT_YIELD	PF_Q20_EQUIVALENT_YIELD
999786	999786	191	191206593	158285782	0.827826	129096953	0.67517	279832461	279832461

## HISTOGRAM	java.lang.Integer
KEY	QUAL_COUNT	MEAN_CYCLE_QUAL	MEDIAN_CYCLE_QUAL	Q25_CYCLE_QUAL	Q75_CYCLE_QUAL
0	2027946	26.241838	26.25	26.25	26.25
1	203296	34.505463	35	34.25	35
2	2980	34.428244	35	34.25	35
3	1118255	33.550177	34.25	32.5	35
4	7244	31.41593	31.75	30	33.5
5	8009	34.696024	35	35	35
6	2789160	33.021726	34.25	32.5	35
7	10216	32.203204	32.5	30	35
8	13200	33.364003	33.75	32.5	35
9	4851274	33.46142	34.25	32.5	35
10	6139	34.147191	35	34.25	35
11	13213	32.479739	33.75	31.75	35
12	5888839	32.873634	35	32.5	35
13	32427	32.769021	35	32.25	35
14	589883	32.857657	35	32.5	35
15	4362258	32.798456	35	32.5	35
16	581781	32.889274	35	32.5	35
17	5014501	32.815366	35	32.5	35
18	111	32.815231	35	32.5	35
19	5400079	32.784683	35	32.5	35
20	252949	32.715389	35	32	35
21	256059	32.603996	35	31.75	35
22	2928635	32.354845	34.25	31.75	35
23	0	31.977953	34.25	31.25	35
24	29314	31.400393	34.25	30.5	35
25	25070341	30.543562	33.75	29.25	35
26	0	29.954821	33.75	28.5	35
27	16	29.170305	33.75	26.25	35
28	170433	28.388275	33	23.5	35
29	481082	27.665903	32.5	20.5	35
30	4561042	27.031062	31.75	18.75	35
31	0	26.490053	30.5	17.75	35
32	8780925	25.964449	29.25	17.25	35
33	0	25.544586	27.5	17	34.25
34	0	25.227842	25.5	17	34.25
35	115754986	24.898018	25	17	34.25
36	0	24.762257	25	17.25	33.75
37	0	24.437477	25	17	33.75
38	0	24.225342	25	17	33.5
39	0	24.023838	24.5	17	33
40	0	23.769146	24	16.75	32.5
41	0	23.622296	23.5	16.5	32.5
42	0	23.445963	23.5	16.5	32.5
43	0	23.338364	23.5	16.25	32.5
44	0	23.270053	23.5	16.25	32.5
45	0	23.233199	23.5	16.25	32.5
46	0	23.201726	23.5	16.25	32.5
47	0	23.26618	23.5	16.25	32.5
48	0	23.335172	23.5	16.25	32.5
49	0	23.306481	23.5	16.25	32.5
50	0	23.316533	23.5	16.5	32.5
51	0	23.269651	23.5	16.5	31.75
52	0	23.282997	23.5	16.5	31.75
53	0	23.140573	23	16.5	31.75
54	0	22.985735	23	16.5	31.75
55	0	22.823325	23	16.25	31
56	0	22.905543	23	16.5	31
57	0	22.872187	23	16.5	30.5
58	0	22.94896	23	16.75	30
59	0	22.903177	23	17	30
60	0	22.850177	23	17	30
61	0	22.855097	23	17	30
62	0	22.917487	23.5	17.25	30
63	0	22.95597	23.5	17.5	29.75
64	0	23.031269	23.5	17.75	29.25
65	0	23.135053	23.5	18	29.5
66	0	23.225317	24	18	30
67	0	23.336203	24.75	18.25	30
68	0	23.407065	25	18.5	30
69	0	23.505366	25	18.5	30
70	0	23.623899	25	18.5	30
71	0	23.644072	25	18.75	30
72	0	23.667633	25	19	30
73	0	23.713843	25	19	30
74	0	23.789229	25	19	30
75	0	23.66385	25	18.75	30
76	0	23.52214	25	18.5	30
77	0	23.264282	25	17.5	30
78	0	23.061949	25	17	30
79	0	22.931096	25	16.666667	30
80	0	23.115972	25	17	30
81	0	23.167717	25	17.25	30
82	0	23.262861	25	17.5	30
83	0	23.41023	25	18	30
84	0	23.347407	25	18	30
85	0	23.338009	25	17.75	30
86	0	23.066288	25	17.25	30
87	0	22.82948	24.5	16.666667	29.75
88	0	23.002628	24.5	16.75	30
89	0	22.861853	24.25	16.666667	30
90	0	22.79764	23.75	16.75	30
91	0	22.94509	24.25	16.75	30
92	0	23.220765	24.5	17	30
93	0	23.331202	24.25	17.5	30
94	0	23.073159	23.5	17	30
95	0	22.926804	23.5	17	29.25
96	0	22.712858	23.25	16.75	29.25
97	0	22.471762	23	16.25	29.25
98	0	22.451224	22.75	16.25	29.25
99	0	22.267454	22.75	16	29.25
100	0	21.919832	22	16	28.5
101	0	21.779111	22	15.5	28
102	0	21.404816	21.5	15.5	27.5
103	0	21.132021	21	15	27.25
104	0	20.571704	20.25	14.75	26
105	0	19.6655	19	14	25
106	0	18.749759	18	13	23.75
107	0	17.739739	17.25	12.5	22.5
108	0	9.939866	9.5	6	12.5