## htsjdk.samtools.metrics.StringHeader
# CollectQualityYieldMetricsFlow INCLUDE_BQ_HISTOGRAM=true INPUT=/Ddata/Runs/441525/output/441525-20260312_0210/441525-L5401-Z0090-CAATATGCACGTGAT/441525-L5401-Z0090-CAATATGCACGTGAT_sample.sam.gz OUTPUT=/Ddata/Runs/441525/output/441525-20260312_0210/441525-L5401-Z0090-CAATATGCACGTGAT/441525-L5401-Z0090-CAATATGCACGTGAT_FlowQ.metric    INCLUDE_SECONDARY_ALIGNMENTS=false INCLUDE_SUPPLEMENTAL_ALIGNMENTS=false IGNORET0TAG=false FILLINGVALUE=0.0 KEEPBOUNDARYFLOWS=false ASSUME_SORTED=true STOP_AFTER=0 VERBOSITY=INFO QUIET=false VALIDATION_STRINGENCY=STRICT COMPRESSION_LEVEL=5 MAX_RECORDS_IN_RAM=500000 CREATE_INDEX=false CREATE_MD5_FILE=false USE_JDK_DEFLATER=false USE_JDK_INFLATER=false
## htsjdk.samtools.metrics.StringHeader
# Started on: Fri Mar 13 02:51:02 EDT 2026

## METRICS CLASS	picard.analysis.CollectQualityYieldMetricsFlow$QualityYieldMetricsFlow
TOTAL_READS	PF_READS	MEAN_READ_LENGTH_IN_FLOWS	PF_FLOWS	PF_Q20_FLOWS	PCT_PF_Q20_FLOWS	PF_Q30_FLOWS	PCT_PF_Q30_FLOWS	Q20_EQUIVALENT_YIELD	PF_Q20_EQUIVALENT_YIELD
1001433	1001433	192	193133490	160848098	0.832834	131974917	0.683335	283871469	283871469

## HISTOGRAM	java.lang.Integer
KEY	QUAL_COUNT	MEAN_CYCLE_QUAL	MEDIAN_CYCLE_QUAL	Q25_CYCLE_QUAL	Q75_CYCLE_QUAL
0	2031767	26.24297	26.25	26.25	26.25
1	196484	34.513888	35	34.25	35
2	3345	34.451065	35	34.25	35
3	1123825	34.110615	34.25	34.25	35
4	6850	31.459049	31.75	30	33.5
5	8392	34.74037	35	35	35
6	2790500	33.062169	34.25	31.75	35
7	9794	32.233073	32.5	30	35
8	13306	33.319158	33.75	32.5	35
9	4769049	33.237513	34.25	32.5	35
10	5932	34.115691	35	34.25	35
11	13545	32.514992	33.75	31.75	35
12	5762666	32.896745	35	32.5	35
13	34677	32.786778	35	32.25	35
14	586222	32.865985	35	32.5	35
15	4253625	32.819325	35	32.5	35
16	581294	32.878523	35	32.5	35
17	4861329	32.810165	35	32.5	35
18	87	32.788185	35	32.5	35
19	5232703	32.76163	35	32.5	35
20	267831	32.691067	35	31.75	35
21	278790	32.565241	35	31.75	35
22	2979874	32.358232	34.25	31.75	35
23	0	32.014373	34.25	31.25	35
24	31136	31.482765	34.25	30.5	35
25	24615022	30.67433	33.75	29.75	35
26	0	30.160931	33.75	28.75	35
27	21	29.468235	33.75	27.25	35
28	186082	28.762177	33.5	25	35
29	514425	28.101702	32.5	22.5	35
30	4777548	27.494665	32.25	20	35
31	0	26.9584	31.75	18.75	35
32	9165891	26.431138	30.25	18	35
33	0	25.986691	29.25	17.75	35
34	0	25.65206	27.5	17.5	34.25
35	118031478	25.304861	26	17.25	34.25
36	0	25.17273	25	17.5	34.25
37	0	24.866746	25	17.25	33.75
38	0	24.657814	25	17.25	33.75
39	0	24.453442	25	17	33.75
40	0	24.189387	25	17	33.75
41	0	24.063972	25	16.75	33.5
42	0	23.867361	24.5	16.75	33.25
43	0	23.73293	24	16.5	33
44	0	23.621998	23.5	16.5	33
45	0	23.565453	23.5	16.25	33
46	0	23.519789	23.5	16.25	32.75
47	0	23.565285	23.5	16.25	32.75
48	0	23.617533	23.5	16.5	32.5
49	0	23.609923	23.5	16.5	32.5
50	0	23.613312	23.5	16.5	32.5
51	0	23.549075	23.5	16.75	32.5
52	0	23.557086	23.5	16.75	32.5
53	0	23.391708	23.5	16.75	31.75
54	0	23.233124	23.5	16.5	31.75
55	0	23.045341	23	16.5	31.75
56	0	23.109653	23.25	16.5	31.75
57	0	23.083562	23	16.75	31.25
58	0	23.143874	23.5	17	31
59	0	23.086125	23.5	17	30.75
60	0	23.026499	23.5	17	30
61	0	23.064076	23.5	17	30
62	0	23.13811	23.5	17.5	30
63	0	23.201992	23.5	17.75	30
64	0	23.271545	23.5	18	30
65	0	23.367857	24	18	30
66	0	23.478924	25	18.25	30
67	0	23.608803	25	18.5	30
68	0	23.644507	25	18.5	30
69	0	23.796699	25	18.75	30
70	0	23.901733	25	19	30
71	0	23.937073	25	19	30
72	0	24.000759	25	19.25	30
73	0	24.051311	25	19.5	30
74	0	24.053734	25	19.5	30
75	0	24.017745	25	19.25	30
76	0	23.875289	25	19	30
77	0	23.655125	25	18	30
78	0	23.552504	25	17.75	30
79	0	23.54036	25	17.5	30
80	0	23.578977	25	17.75	30
81	0	23.658716	25	18	30
82	0	23.656076	25	18.25	30
83	0	23.627758	25	18.25	30
84	0	23.648437	25	18.25	30
85	0	23.666362	25	18.25	30
86	0	23.513339	25	17.75	30
87	0	23.306931	25	17.25	30
88	0	23.222212	25	17.25	30
89	0	23.129815	24.5	17.25	30
90	0	23.326303	24.5	17.5	30
91	0	23.212875	24.5	17.75	30
92	0	23.10311	24.25	17.25	30
93	0	23.374326	24.25	17.75	30
94	0	23.230511	24	17.25	30
95	0	23.131664	24.25	17	30
96	0	22.770992	23.5	16.75	29.75
97	0	22.809519	23.5	16.75	29.25
98	0	22.681475	23	16.5	29.25
99	0	22.58955	22.75	16.5	29.25
100	0	22.276393	22.75	16.25	28.75
101	0	21.852908	22	15.75	28.5
102	0	21.523895	21.75	15.5	27.5
103	0	21.304067	21	15.5	27.25
104	0	20.79823	20.5	15	26
105	0	19.846878	19.5	14.5	25
106	0	18.887549	18.25	13.5	24.25
107	0	17.65618	17	12.75	22.25
108	0	9.994531	9.5	6	12.5