## htsjdk.samtools.metrics.StringHeader
# CollectQualityYieldMetricsFlow INCLUDE_BQ_HISTOGRAM=true INPUT=/Ddata/Runs/441525/output/441525-20260312_0210/441525-L5399-Z0088-CTATCATCCAGTGAT/441525-L5399-Z0088-CTATCATCCAGTGAT_sample.sam.gz OUTPUT=/Ddata/Runs/441525/output/441525-20260312_0210/441525-L5399-Z0088-CTATCATCCAGTGAT/441525-L5399-Z0088-CTATCATCCAGTGAT_FlowQ.metric    INCLUDE_SECONDARY_ALIGNMENTS=false INCLUDE_SUPPLEMENTAL_ALIGNMENTS=false IGNORET0TAG=false FILLINGVALUE=0.0 KEEPBOUNDARYFLOWS=false ASSUME_SORTED=true STOP_AFTER=0 VERBOSITY=INFO QUIET=false VALIDATION_STRINGENCY=STRICT COMPRESSION_LEVEL=5 MAX_RECORDS_IN_RAM=500000 CREATE_INDEX=false CREATE_MD5_FILE=false USE_JDK_DEFLATER=false USE_JDK_INFLATER=false
## htsjdk.samtools.metrics.StringHeader
# Started on: Fri Mar 13 02:51:37 EDT 2026

## METRICS CLASS	picard.analysis.CollectQualityYieldMetricsFlow$QualityYieldMetricsFlow
TOTAL_READS	PF_READS	MEAN_READ_LENGTH_IN_FLOWS	PF_FLOWS	PF_Q20_FLOWS	PCT_PF_Q20_FLOWS	PF_Q30_FLOWS	PCT_PF_Q30_FLOWS	Q20_EQUIVALENT_YIELD	PF_Q20_EQUIVALENT_YIELD
1001238	1001238	198	198516482	165644100	0.83441	136595457	0.688081	292516599	292516599

## HISTOGRAM	java.lang.Integer
KEY	QUAL_COUNT	MEAN_CYCLE_QUAL	MEDIAN_CYCLE_QUAL	Q25_CYCLE_QUAL	Q75_CYCLE_QUAL
0	2032495	26.241583	26.25	26.25	26.25
1	196935	34.393333	35	34.25	35
2	3888	34.375078	35	34.25	35
3	1153936	34.037136	34.25	34.25	35
4	8437	32.07847	32.5	31	34.25
5	9307	34.778968	35	35	35
6	2840562	33.245473	34.25	32.5	35
7	10981	32.441887	33.75	31	35
8	14661	33.289528	33.75	32.5	35
9	4830664	33.349163	34.25	32.5	35
10	6566	34.210822	35	34.25	35
11	13953	32.600822	33.75	31.75	35
12	5875621	32.981391	35	32.5	35
13	34340	32.848676	35	32.5	35
14	597227	32.90532	35	32.5	35
15	4343328	32.85772	35	32.5	35
16	591906	32.920592	35	32.5	35
17	4970206	32.837563	35	32.5	35
18	98	32.824032	35	32.5	35
19	5337271	32.804773	35	32.5	35
20	260480	32.738539	35	32.5	35
21	283442	32.62372	35	31.75	35
22	3058363	32.410863	35	31.75	35
23	0	32.085119	34.25	31.75	35
24	30914	31.562823	34.25	31	35
25	24718620	30.789401	33.75	30	35
26	0	30.24376	33.75	29	35
27	14	29.553821	33.75	27.5	35
28	188588	28.856244	33.5	25	35
29	508222	28.203079	32.5	22.75	35
30	4943096	27.626788	32.5	20.25	35
31	0	27.126854	31.75	19	35
32	9161481	26.651175	31	18.25	35
33	0	26.249257	30	18	35
34	0	25.964869	28.5	17.75	35
35	122490880	25.654227	27	17.75	34.25
36	0	25.540807	26	17.75	34.25
37	0	25.248306	25	17.75	34.25
38	0	25.060064	25	17.75	34.25
39	0	24.87927	25	17.5	33.75
40	0	24.663927	25	17.25	33.75
41	0	24.550929	25	17.25	33.75
42	0	24.412562	25	17	33.75
43	0	24.341344	25	17	33.75
44	0	24.290653	25	17	33.75
45	0	24.25084	25	16.75	33.75
46	0	24.251777	25	16.75	33.75
47	0	24.325641	25	17	33.75
48	0	24.408824	25	17	33.75
49	0	24.413563	25	17	33.75
50	0	24.414941	25	17	33.75
51	0	24.347231	25	17	33.75
52	0	24.329001	25	17.25	33.75
53	0	24.167823	25	17	33.5
54	0	23.993084	24.75	17	33
55	0	23.784571	24	16.75	32.5
56	0	23.821669	24	17	32.5
57	0	23.763176	23.5	17	32.5
58	0	23.802065	24	17.25	32.5
59	0	23.694652	23.5	17.25	32.25
60	0	23.620496	23.5	17.25	31.75
61	0	23.597582	23.5	17.5	31.75
62	0	23.57127	23.5	17.75	31
63	0	23.571793	23.5	17.75	30.5
64	0	23.589795	24	18	30
65	0	23.678052	24.75	18.25	30
66	0	23.74204	25	18.5	30
67	0	23.7613	25	18.5	30
68	0	23.856692	25	18.75	30
69	0	23.95278	25	19	30
70	0	24.033387	25	19	30
71	0	24.057755	25	19.25	30
72	0	24.064115	25	19.5	30
73	0	24.076883	25	19.5	30
74	0	24.084954	25	19.5	30
75	0	23.941725	25	19	30
76	0	23.904753	25	19	30
77	0	23.736787	25	18.25	30
78	0	23.512727	25	17.75	30
79	0	23.530723	25	17.5	30
80	0	23.629083	25	18	30
81	0	23.606831	25	18	30
82	0	23.709317	25	18	30
83	0	23.738264	25	18.5	30
84	0	23.753372	25	18.5	30
85	0	23.68939	25	18	30
86	0	23.569952	25	17.75	30
87	0	23.471397	25	17.75	30
88	0	23.449973	25	17.5	30
89	0	23.378036	25	17.5	30
90	0	23.365582	24.75	17.5	30
91	0	23.374873	24.5	17.5	30
92	0	23.366674	24.5	17.75	30
93	0	23.548559	24.5	17.75	30
94	0	23.277206	24.25	17.25	30
95	0	23.201307	23.75	17.25	30
96	0	23.070097	23.5	17	30
97	0	22.876425	23.5	17	29.25
98	0	22.60444	23	16.666667	29.25
99	0	22.567111	23	16.5	29.25
100	0	22.368065	22.75	16.25	29.25
101	0	22.046625	22	16	28.5
102	0	21.476233	21.25	15.5	27.5
103	0	21.200245	21	15.25	26.75
104	0	20.769784	20.25	15.25	26.25
105	0	19.682778	19.25	14	25
106	0	18.868608	18	13.25	24.25
107	0	17.803582	17.5	12.75	22.5
108	0	10.123192	9.5	6	12.5