## htsjdk.samtools.metrics.StringHeader
# CollectQualityYieldMetricsFlow INCLUDE_BQ_HISTOGRAM=true INPUT=/Ddata/Runs/441525/output/441525-20260312_0210/441525-L5398-Z0086-CAACTATCTGCAGAT/441525-L5398-Z0086-CAACTATCTGCAGAT_sample.sam.gz OUTPUT=/Ddata/Runs/441525/output/441525-20260312_0210/441525-L5398-Z0086-CAACTATCTGCAGAT/441525-L5398-Z0086-CAACTATCTGCAGAT_FlowQ.metric    INCLUDE_SECONDARY_ALIGNMENTS=false INCLUDE_SUPPLEMENTAL_ALIGNMENTS=false IGNORET0TAG=false FILLINGVALUE=0.0 KEEPBOUNDARYFLOWS=false ASSUME_SORTED=true STOP_AFTER=0 VERBOSITY=INFO QUIET=false VALIDATION_STRINGENCY=STRICT COMPRESSION_LEVEL=5 MAX_RECORDS_IN_RAM=500000 CREATE_INDEX=false CREATE_MD5_FILE=false USE_JDK_DEFLATER=false USE_JDK_INFLATER=false
## htsjdk.samtools.metrics.StringHeader
# Started on: Fri Mar 13 02:50:23 EDT 2026

## METRICS CLASS	picard.analysis.CollectQualityYieldMetricsFlow$QualityYieldMetricsFlow
TOTAL_READS	PF_READS	MEAN_READ_LENGTH_IN_FLOWS	PF_FLOWS	PF_Q20_FLOWS	PCT_PF_Q20_FLOWS	PF_Q30_FLOWS	PCT_PF_Q30_FLOWS	Q20_EQUIVALENT_YIELD	PF_Q20_EQUIVALENT_YIELD
1000302	1000302	196	196247641	163370945	0.832473	133842665	0.682009	288410361	288410361

## HISTOGRAM	java.lang.Integer
KEY	QUAL_COUNT	MEAN_CYCLE_QUAL	MEDIAN_CYCLE_QUAL	Q25_CYCLE_QUAL	Q75_CYCLE_QUAL
0	2030269	26.24056	26.25	26.25	26.25
1	198785	34.040298	34.25	34.25	35
2	3469	34.622156	35	34.25	35
3	1152260	33.903174	34.25	32.5	35
4	7421	31.442432	31.75	30	33.5
5	8397	34.765595	35	35	35
6	2845001	32.964436	34.25	32.5	35
7	10190	32.338047	33.75	30.25	35
8	13476	33.296909	33.75	32.5	35
9	4827072	33.478885	34.25	32.5	35
10	6101	34.229792	35	34.25	35
11	13230	32.640478	33.75	31.75	35
12	5837728	32.972153	35	32.5	35
13	34020	32.826879	35	32.5	35
14	587482	32.918928	35	32.5	35
15	4356610	32.857617	35	32.5	35
16	590353	32.920721	35	32.5	35
17	4983593	32.835781	35	32.5	35
18	80	32.817059	35	32.5	35
19	5371159	32.791499	35	32.5	35
20	257991	32.724675	35	32.25	35
21	276648	32.609816	35	31.75	35
22	3041511	32.376406	35	31.75	35
23	0	32.019604	34.25	31.5	35
24	30461	31.459796	34.25	30.75	35
25	25234262	30.633014	33.75	29.75	35
26	0	30.118171	33.75	28.75	35
27	21	29.41779	33.75	27	35
28	186848	28.731187	33.5	25	35
29	500538	28.108857	32.5	22	35
30	4802622	27.554867	32.5	20.25	35
31	0	27.081285	31.75	19	35
32	8804576	26.602529	30.5	18.5	35
33	0	26.204519	29.25	18	35
34	0	25.901368	27.75	18	35
35	120235467	25.569879	26.75	17.75	34.25
36	0	25.423751	25.25	17.75	34.25
37	0	25.112885	25	17.75	34.25
38	0	24.922203	25	17.5	33.75
39	0	24.726985	25	17.5	33.75
40	0	24.491014	25	17.25	33.75
41	0	24.340598	25	17	33.75
42	0	24.160302	25	17	33.75
43	0	24.043302	25	16.75	33.5
44	0	23.933385	25	16.75	33.5
45	0	23.862917	24.75	16.5	33.5
46	0	23.821087	24.5	16.5	33.5
47	0	23.848317	24.5	16.5	33.25
48	0	23.875316	24.5	16.5	33
49	0	23.856643	24.5	16.75	33
50	0	23.842824	24.5	16.75	33
51	0	23.746968	23.75	16.75	32.5
52	0	23.744011	23.5	17	32.5
53	0	23.58243	23.5	16.75	32.5
54	0	23.417282	23.5	16.666667	32.25
55	0	23.241988	23.5	16.5	31.75
56	0	23.299899	23.5	16.75	31.75
57	0	23.239341	23.5	16.75	31.75
58	0	23.293549	23.5	17	31.75
59	0	23.229324	23.5	17	31.25
60	0	23.18727	23.5	17	30.5
61	0	23.195188	23.5	17.25	30
62	0	23.238242	23.5	17.5	30
63	0	23.287275	23.5	17.75	30
64	0	23.388278	23.5	18	30
65	0	23.490319	24.25	18.25	30
66	0	23.591025	25	18.5	30
67	0	23.661392	25	18.5	30
68	0	23.744787	25	18.5	30
69	0	23.81855	25	18.75	30
70	0	23.928264	25	19	30
71	0	23.981315	25	19	30
72	0	24.031988	25	19.25	30
73	0	24.07179	25	19.5	30
74	0	24.110273	25	19.5	30
75	0	23.978946	25	19	30
76	0	23.86255	25	18.75	30
77	0	23.666416	25	18	30
78	0	23.466711	25	17.5	30
79	0	23.404124	25	17.25	30
80	0	23.542174	25	17.5	30
81	0	23.653765	25	18	30
82	0	23.762448	25	18.5	30
83	0	23.793191	25	18.5	30
84	0	23.833743	25	18.5	30
85	0	23.836283	25	18.5	30
86	0	23.628857	25	18	30
87	0	23.425161	25	17.5	30
88	0	23.512371	25	17.75	30
89	0	23.439737	25	17.5	30
90	0	23.528131	25	17.75	30
91	0	23.539361	25	17.75	30
92	0	23.45806	25	17.75	30
93	0	23.632353	25	18	30
94	0	23.464008	24.5	17.75	30
95	0	23.276322	24	17.25	30
96	0	23.129216	23.5	17	30
97	0	22.994323	23.5	17	30
98	0	22.955848	23.5	17	30
99	0	22.813301	23.25	17	29.25
100	0	22.54013	22.75	16.5	29.25
101	0	22.208432	22.5	16	28.75
102	0	21.892978	22	16	27.75
103	0	21.4171	21	15.5	27.25
104	0	21.063481	20.5	15.25	26.75
105	0	20.173387	19.75	14.5	25.25
106	0	19.099281	18.5	13.5	24.25
107	0	17.892935	17.5	12.75	22.75
108	0	10.129545	9.5	6	12.5